Research Topics
Species | Harold RobertsSummaryAffiliation: University of North Carolina Country: USA Publications
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Publications
Clinical experience with activated factor VII: focus on safety aspectsH R Roberts
University of North Carolina School of Medicine, Division of Hematology Oncology, Chapel Hill 27599 7035, USA
Blood Coagul Fibrinolysis 9:S115-8. 1998..Analysis of these events on a case-by-case basis suggests that rFVIIa is a very safe product with very few side effects. In particular, thromboembolic complications have occurred rarely, if at all...
The use of recombinant factor VIIa in the treatment of bleeding disordersHarold R Roberts
Division of Hematology Oncology, Department of Medicine and The Carolina Cardiovascular Biology Center, 932 Mary Ellen Jones Bldg, Chapel Hill, NC 27599 7035, USA
Blood 104:3858-64. 2004..Several questions pertaining to the use of factor VIIa require further investigation, including the mechanism of action; the optimal dose; definitive indications; ultimate safety; and laboratory tests for monitoring therapy...
Current concepts of hemostasis: implications for therapyHarold R Roberts
Division of Hematology Oncology, Carolina Cardiovascular Biology Center, University of North Carolina School of Medicine, Chapel Hill 27599 7035, USA
Anesthesiology 100:722-30. 2004..Finally, recombinant factor VIIa has been used off-label as a hemostatic agent in some surgical situations associated with excessive bleeding that is not responsive to conventional therapy...
A cell-based model of thrombin generationHarold R Roberts
School of Medicine, Division of Hematology Oncology, Carolina Cardiovascular Biology Center, University of North Carolina, Chapel Hill, North Carolina 27599 7035, USA
Semin Thromb Hemost 32:32-8. 2006..We have also shown that activated protein C does not inactivate Va on the platelet surface but rather on endothelial cell surfaces...
Recombinant factor VIIa: a general hemostatic agent? YesH R Roberts
Division of Hematology Oncology, Department of Medicine and The Carolina Cardiovascular Biology Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7035, USA
J Thromb Haemost 2:1691-4. 2004
Contributions to the evolution of knowledge about hereditary hemorrhagic disordersH R Roberts
University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599-7035, USA
Cell Mol Life Sci 64:517-21. 2007
Manipulation of prothrombin concentration improves response to high-dose factor VIIa in a cell-based model of haemophiliaGeoffrey A Allen
Department of Pediatrics, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA
Br J Haematol 134:314-9. 2006..Furthermore, these results suggest that increasing plasma prothrombin concentration prior to rFVIIa administration may offer advantages over the use of rFVIIa alone in the treatment of haemophilic bleeding...
Safety profile of recombinant factor VIIaHarold R Roberts
Division of Hematology, Center for Thrombosis and Hemostasis, University of North Carolina at Chapel Hill, USA
Semin Hematol 41:101-8. 2004..Finally, those situations in which rFVIIa therapy may not be safe, such as disseminated intravascular coagulation (DIC) and sepsis, will also be discussed...
Perivascular tissue factor is down-regulated following cutaneous wounding: implications for bleeding in hemophiliaAnna G McDonald
Duke University, Durham, NC, USA
Blood 111:2046-8. 2008..Thus, perivascular TF expression is down-regulated during wound healing. This may prevent thrombosis of neovessels during angiogenesis but renders hemophiliacs vulnerable to hemorrhage during healing...
Replacing the first epidermal growth factor-like domain of factor IX with that of factor VII enhances activity in vitro and in canine hemophilia BJ Y Chang
Center for Thrombosis and Hemostasis, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7035, USA
J Clin Invest 100:886-92. 1997....
Cloning, expression, and characterization of mouse tissue factor pathway inhibitor (TFPI)J Y Chang
Center for Thrombosis and Hemostasis, University of North Carolina at Chapel Hill, 27599 7035, USA
Thromb Haemost 79:306-9. 1998..Cloning and expression of the mouse TFPI gene will offer useful information and material for coagulation studies performed in a mouse model system...
Impact of procoagulant concentration on rate, peak and total thrombin generation in a model systemG A Allen
Department of Pediatrics Department of Pathology and Laboratory Medicine Department of Medicine Carolina Cardiovascular Biology Center, University of North Carolina, Chapel Hill, NC, USA
J Thromb Haemost 2:402-13. 2004....
TFPIbeta, a second product from the mouse tissue factor pathway inhibitor (TFPI) geneJ Y Chang
Center for Thrombosis and Hemostasis, University of North Carolina, Chapel Hill, USA
Thromb Haemost 81:45-9. 1999..In addition, heparin does not enhance the inhibition of factor Xa by mouse TFPIbeta although it does accelerate factor Xa inhibition by TFPI...
A possible mechanism of action of activated factor VII independent of tissue factorD M Monroe
Department of Medicine, University of North Carolina, Chapel Hill 27599 7035, USA
Blood Coagul Fibrinolysis 9:S15-20. 1998..We speculate that this platelet-localized thrombin generation may, in part, account for the clinical efficacy of high-dose FVIIa...
Activated factor VII activates factors IX and X on the surface of activated platelets: thoughts on the mechanism of action of high-dose activated factor VIIM Hoffman
Department of Pathology, Durham Veterans Affairs Medical Center, NC 27705, USA
Blood Coagul Fibrinolysis 9:S61-5. 1998..Activation of FIX and FX on platelet surfaces in thrombocytopenia would result in more thrombin generation per platelet, possibly leading to formation of a stable fibrin network even in the absence of an optimal initial platelet plug...
Recombinant human factor VIIa (rFVIIa) can activate factor FIX on activated plateletsD A Gabriel
Division of Hematology Oncology, The Bone Marrow Transplant Program and The University of North Carolina School of Medicine, Carolina Center for Cardiovascular Biology, Chapel Hill, NC 27599, USA
J Thromb Haemost 2:1816-22. 2004....
The factor VII-platelet interplay: effectiveness of recombinant factor VIIa in the treatment of bleeding in severe thrombocytopathiaD M Monroe
Center for Thrombosis and Hemostasis, UNC School of Medicine, Chapel Hill, North Carolina, USA
Semin Thromb Hemost 26:373-7. 2000..Finally, enhanced thrombin generation on platelets would mean that fewer platelets are required for hemostasis...
Rationale for the use of high dose rFVIIa in a high-titre inhibitor patient with haemophilia B during major orthopaedic proceduresH A Cooper
Department of Paediatrics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7220
Haemophilia 7:517-22. 2001....
Recombinant factor VIIa (Novoseven) and the safety of treatmentH R Roberts
University of North Carolina, Chapel Hill, NC, USA
Semin Hematol 38:48-50. 2001..Moreover, in the majority of cases, thrombotic events appear to be caused by improvements in the hemostatic system, rather than a direct effect of the agent itself...
Elevated prothrombin results in clots with an altered fiber structure: a possible mechanism of the increased thrombotic riskAlisa S Wolberg
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, USA
Blood 101:3008-13. 2003..We hypothesize that this alteration in fibrin clot structure is an important determinant of the risk of thrombosis...
Recombinant factor VIIa: how safe is the stuff?Harold R Roberts
Can J Anaesth 52:8-11. 2005
Platelet heterogeneity: variation in coagulation complexes on platelet subpopulationsChristine L Kempton
University of North Carolina, Chapel Hill, NC 27599-7035, USA
Arterioscler Thromb Vasc Biol 25:861-6. 2005..Convulxin-induced increases in thrombin generation are regulated by the proportion of platelets with increased coagulation factor binding...
Fathers of modern coagulationDougald M Monroe
UNC Medical School--Hematology/Oncology, 932 Mary Ellen Jones, CB#7035 Chapel Hill, North Carolina 27599-7035, USA
Thromb Haemost 98:3-5. 2007
High dose factor VIIa improves clot structure and stability in a model of haemophilia BAlisa S Wolberg
Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, NC 27599 7525, USA
Br J Haematol 131:645-55. 2005....
Novel therapeutic agents in the management of hemorrhage and thrombosisChristine L Kempton
Aflac Cancer Center and Blood Disorders Service and Winship Cancer Institute, Emory University, Atlanta, GA, USA
Cardiovasc Hematol Agents Med Chem 4:319-34. 2006..These agents and others will be discussed in detail with respect to mechanism of action, clinical efficacy and safety...
Cutaneous wound healing is impaired in hemophilia BMaureane Hoffman
Laboratory Service 113, Durham Veterans Affairs Medical Center, 508 Fulton St, Durham, NC 27705, USA
Blood 108:3053-60. 2006..Our findings have significant implications for treatment of patients with hemophilia, and also highlight the importance of rapidly establishing hemostasis following trauma or surgery...
Activated protein C cleaves factor Va more efficiently on endothelium than on platelet surfacesJulie A Oliver
Duke University and the Durham VA Medical Hospital, Durham, NC, USA
Blood 100:539-46. 2002..From the studies reported here, we conclude that in vivo, despite the important role of the protein C system in regulating thrombosis, activated protein C does not serve as a primary regulator of platelet-dependent thrombin generation...
Platelets and thrombin generationDougald M Monroe
Center for Thrombosis and Hemostasis, University of North Carolina, Chapel Hill 27599 7035, USA
Arterioscler Thromb Vasc Biol 22:1381-9. 2002..Individual differences in platelet thrombin generation might be accounted for by differences in platelet-binding proteins...
Mechanism of action of high-dose factor VIIa: points of agreement and disagreementDougald M Monroe
Arterioscler Thromb Vasc Biol 23:8-9; discussion 10. 2003
Recombinant activated factor VII: its mechanism of action and role in the control of hemorrhageGeoffrey A Allen
Center for Thrombosis and Hemostasis and Pediatric Hematology Oncology, University of North Carolina School of Medicine, Chapel Hill 27599 7220, USA
Can J Anaesth 49:S7-14. 2002..We have attempted to reconcile the various proposed mechanisms of action of rFVIIa with its apparent efficacy in such diverse clinical settings...
Monitoring coagulation and the clinical effects of recombinant factor VIIaDon A Gabriel
Division of Hematology, University of North Carolina, Chapel Hill, MC, USA
Semin Hematol 41:20-4. 2004..Using clinical examples, the utility of these technologies is assessed and conclusions drawn about the most useful diagnostic measures for monitoring hemostasis...
Circulating and binding characteristics of wild-type factor IX and certain Gla domain mutants in vivoTong Gui
Department of Biology, University of North Carolina at Chapel Hill, 27599 3280, USA
Blood 100:153-8. 2002..Our results suggest that factor IX Gla-domain mediated binding to endothelial cells/collagen IV plays a role in controlling factor IX concentration in the blood...
Oscar Ratnoff: his contributions to the golden era of coagulation researchHarold R Roberts
Center for Thrombosis and Hemostasis, University of North Carolina, 932 Mary Ellen Jones Building/Campus Box 7035, Chapel Hill, NC, USA
Br J Haematol 122:180-92. 2003
Platelet-dependent action of high-dose factor VIIaMaureane Hoffman
Blood 100:364-5; author reply 365. 2002
Thrombin generation in vascular tissueA Pathak
Division of Cardiology, Hematology and Cardiothoracic Surgery and Carolina Cardiovascular Biology Center, University of North Carolina, Chapel Hill, NC 27599 7075, USA
J Thromb Haemost 4:60-7. 2006..Classically, it is thought that the vast majority of thrombin is generated on the surface of platelets, however, thrombotic events occur in patients despite treatment with potent antiplatelet agents...
Hemophilia care in the 21st centuryChristine L Kempton
Division of Hematology/Oncology at the University of North Carolina, Chapell Hill, NC 27599-7035, USA
Clin Adv Hematol Oncol 2:733-40. 2004
Functional consequences of an arginine180 to glutamine mutation in factor IX HiloD M Monroe
Department of Medicine, University of North Carolina, Chapel Hill
Blood 73:1540-4. 1989..Further, our data suggest that the mutation results in a molecule that interacts with components of the extrinsic pathway to give a prolonged ox brain prothrombin time...
Overview of anticoagulant drugs for the futureStephan Moll
Department of Medicine, Division of Hematology-Oncology, Center for Thrombosis and Hemostasis, University of North Carolina School of Medicine, Chapel Hill, NC 27599-7035, USA
Semin Hematol 39:145-57. 2002....
More information on patients with factor XI deficiencyHarold R Roberts
Anesthesiology 101:1253-4. 2004
Vitamin K supplementation during oral anticoagulation: concernsDaniel W Stafford
Blood 109:3607; author reply 3607-8. 2007
A study of the pharmacokinetics and safety of recombinant activated factor VII in healthy Caucasian and Japanese subjectsMarianne J Fridberg
Novo Nordisk A S, Krogshoejvej 55, DK 2880 Bagsvaerd, Denmark
Blood Coagul Fibrinolysis 16:259-66. 2005..In conclusion, the pharmacokinetics of rFVIIa in Caucasian and Japanese subjects are similar, and no safety issues were identified...
Research Grants
- CELL MEDIATED HEMOSTASISHarold Roberts; Fiscal Year: 1999....
- CELL MEDIATED HEMOSTASISHarold Roberts; Fiscal Year: 1993....
- CELL MEDIATED HEMOSTASISHarold Roberts; Fiscal Year: 2004....
