Paul D Robbins

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. ncbi request reprint Dendritic cells transduced to express interleukin-4 prevent diabetes in nonobese diabetic mice with advanced insulitis
    Maryam Feili-Hariri
    Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Hum Gene Ther 14:13-23. 2003
  2. ncbi request reprint Prevention of diabetes in NOD mice by administration of dendritic cells deficient in nuclear transcription factor-kappaB activity
    Linlin Ma
    Thomas E Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Diabetes 52:1976-85. 2003
  3. ncbi request reprint Effective treatment of inflammatory disease models with exosomes derived from dendritic cells genetically modified to express IL-4
    Seon Hee Kim
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261
    J Immunol 179:2242-9. 2007
  4. pmc B7-1/2, but not PD-L1/2 molecules, are required on IL-10-treated tolerogenic DC and DC-derived exosomes for in vivo function
    MELANIE A RUFFNER
    Department of Microbiology and Molecular Genetics, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Eur J Immunol 39:3084-90. 2009
  5. pmc SIRT1 negatively regulates the mammalian target of rapamycin
    Hiyaa Singhee Ghosh
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 5:e9199. 2010
  6. pmc Dendritic cells transduced to express interleukin 4 reduce diabetes onset in both normoglycemic and prediabetic nonobese diabetic mice
    MELANIE A RUFFNER
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 5:e11848. 2010
  7. pmc Identification of a cardiac specific protein transduction domain by in vivo biopanning using a M13 phage peptide display library in mice
    Maliha Zahid
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 5:e12252. 2010
  8. pmc Exosomes released from Mycoplasma infected tumor cells activate inhibitory B cells
    Chenjie Yang
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    PLoS ONE 7:e36138. 2012
  9. pmc Adverse effects of adenovirus-mediated gene transfer of human transforming growth factor beta 1 into rabbit knees
    Zhibao Mi
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Arthritis Res Ther 5:R132-9. 2003
  10. pmc Adenoviral-mediated, intratumor gene transfer of interleukin 23 induces a therapeutic antitumor response
    J Reay
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Cancer Gene Ther 16:776-85. 2009

Research Grants

  1. LMP and Bone Healing
    Paul Robbins; Fiscal Year: 2009
  2. Inhibition of NF-KB to Facilitate Islet Transplantation
    Paul Robbins; Fiscal Year: 2003
  3. REGULATION OF TRANSCRIPTION BY THE RB ANTIONCOGENE
    Paul Robbins; Fiscal Year: 2002
  4. REGULATION OF TRANSCRIPTION BY THE RB ANTIONCOGENE
    Paul Robbins; Fiscal Year: 2001
  5. REGULATION OF TRANSCRIPTION BY THE RB ANTIONCOGENE
    Paul Robbins; Fiscal Year: 2000
  6. REGULATION OF TRANSCRIPTION-RETINOBLASTOMA ANTI-ONCOGENE
    Paul Robbins; Fiscal Year: 1993
  7. REGULATION OF TRANSCRIPTION-RETINOBLASTOMA ANTI-ONCOGENE
    Paul Robbins; Fiscal Year: 1992
  8. REGULATION OF TRANSCRIPTION-RETINOBLASTOMA ANTI-ONCOGENE
    Paul Robbins; Fiscal Year: 1991
  9. REGULATION OF TRANSCRIPTION BY THE RB ANTIONCOGENE
    Paul Robbins; Fiscal Year: 1999
  10. LMP and Bone Healing
    Paul D Robbins; Fiscal Year: 2010

Collaborators

Detail Information

Publications103 found, 100 shown here

  1. ncbi request reprint Dendritic cells transduced to express interleukin-4 prevent diabetes in nonobese diabetic mice with advanced insulitis
    Maryam Feili-Hariri
    Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Hum Gene Ther 14:13-23. 2003
    ..IL-4 DC, and these mice were significantly protected from disease. These results demonstrate, for the first time, that genetically modified DC can prevent diabetes in the context of advanced insulitis...
  2. ncbi request reprint Prevention of diabetes in NOD mice by administration of dendritic cells deficient in nuclear transcription factor-kappaB activity
    Linlin Ma
    Thomas E Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Diabetes 52:1976-85. 2003
    ..These findings provide novel insights into the mechanisms of autoimmune diabetes and may lead to development of novel preventive strategies...
  3. ncbi request reprint Effective treatment of inflammatory disease models with exosomes derived from dendritic cells genetically modified to express IL-4
    Seon Hee Kim
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261
    J Immunol 179:2242-9. 2007
    ..Thus, APC-derived exosomes could be used therapeutically for the treatment of autoimmune disease and inflammatory disorders...
  4. pmc B7-1/2, but not PD-L1/2 molecules, are required on IL-10-treated tolerogenic DC and DC-derived exosomes for in vivo function
    MELANIE A RUFFNER
    Department of Microbiology and Molecular Genetics, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Eur J Immunol 39:3084-90. 2009
    ..We conclude that B7-1 and B7-2, but not PD-L1 and PD-L2, on IL-10-treated DC and DC-derived exosomes play a critical role in immunosuppressive functions of both DC and exosomes...
  5. pmc SIRT1 negatively regulates the mammalian target of rapamycin
    Hiyaa Singhee Ghosh
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 5:e9199. 2010
    ..These results demonstrate that SIRT1 negatively regulates mTOR signaling potentially through the TSC1/2 complex...
  6. pmc Dendritic cells transduced to express interleukin 4 reduce diabetes onset in both normoglycemic and prediabetic nonobese diabetic mice
    MELANIE A RUFFNER
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 5:e11848. 2010
    ..However, the mechanism(s) through which DC expressing IL-4 function to prevent autoimmune diabetes and whether this treatment can reverse disease in pre-diabetic NOD mice are unknown...
  7. pmc Identification of a cardiac specific protein transduction domain by in vivo biopanning using a M13 phage peptide display library in mice
    Maliha Zahid
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 5:e12252. 2010
    ..In order to identify peptide(s) able to transduce heart tissue, biopanning was performed in cell culture and in vivo with a M13 phage peptide display library...
  8. pmc Exosomes released from Mycoplasma infected tumor cells activate inhibitory B cells
    Chenjie Yang
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    PLoS ONE 7:e36138. 2012
    ..These results suggest that mycoplasmas infecting tumor cells can exploit the exosome pathway to disseminate their own components and modulate the activity of immune cells, in particular, activate B cells with inhibitory activity...
  9. pmc Adverse effects of adenovirus-mediated gene transfer of human transforming growth factor beta 1 into rabbit knees
    Zhibao Mi
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Arthritis Res Ther 5:R132-9. 2003
    ..Gene transfer of TGF-beta1 to the synovium is thus not suitable for treating intra-articular pathologies...
  10. pmc Adenoviral-mediated, intratumor gene transfer of interleukin 23 induces a therapeutic antitumor response
    J Reay
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Cancer Gene Ther 16:776-85. 2009
    ..These results demonstrate that direct intratumoral injection of adenovirus expressing IL-23 results in enhanced survival, tumor eradication and generation of protective immunity by generation of a Th1-type immune response...
  11. pmc The roles of tumor-derived exosomes in cancer pathogenesis
    Chenjie Yang
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA
    Clin Dev Immunol 2011:842849. 2011
    ..These different effects of tumor-derived exosomes contribute to the pathogenesis of cancer. This review will discuss the roles of tumor-derived exosomes in cancer pathogenesis, therapy, and diagnostics...
  12. pmc SIRT1 associates with eIF2-alpha and regulates the cellular stress response
    Hiyaa Singhee Ghosh
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA Department of Microbiology and Immunology, Columbia University Medical Center, New York, NY 10032, USA
    Sci Rep 1:150. 2011
    ..Finally, SIRT1 co-immunoprecipitates with mediators of eIF2α dephosphorylation, GADD34 and CreP, suggesting a role for SIRT1 in the negative feedback regulation of eIF2α phosphorylation...
  13. pmc Immunosuppressive exosomes: a new approach for treating arthritis
    Chenjie Yang
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA
    Int J Rheumatol 2012:573528. 2012
    ..This paper discusses the current knowledge on the production, efficacy, mechanism of action, and potential therapeutic use of immunosuppressive exosomes for arthritis therapy...
  14. pmc Dedifferentiation rescues senescence of progeria cells but only while pluripotent
    Laura J Niedernhofer
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, 523 Bridgeside Point II, 450 Technology Drive, Pittsburgh, PA 15219, USA
    Stem Cell Res Ther 2:28. 2011
    ..These new HGPS iPSCs are valuable for characterizing the role of progerin in driving HGPS and aging and for screening therapeutic strategies to prevent or delay cell senescence...
  15. pmc Gene therapy of the rheumatic diseases: 1998 to 2008
    Christopher H Evans
    Center for Advanced Orthopaedic Studies, Harvard Medical School, BIDMC RN115, 330 Brookline Avenue, Boston, MA 02215, USA
    Arthritis Res Ther 11:209. 2009
    ..The translational research necessary to turn these advances into effective genetic medicines requires sustained funding and continuity of effort...
  16. pmc Arthritis gene therapy's first death
    Christopher H Evans
    Center for Molecular Orthopaedics, Harvard Medical School, 221 Longwood Avenue, BLI 152, Boston, MA 02115, USA
    Arthritis Res Ther 10:110. 2008
    ..In the present article we collate the information we were able to obtain about this clinical trial and discuss it in the wider context of arthritis gene therapy...
  17. pmc Gene combination transfer to block autoimmune damage in transplanted islets of Langerhans
    Suzanne Bertera
    Division of Immunogenetics, Department of Pediatrics, University of Pittsburgh School of Medicine, Rangos Research Center, Children s Hospital of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Exp Diabesity Res 5:201-10. 2004
    ..Although the results of these cotransfections gave little evidence of a synergistic relationship, they were useful to show that gene combinations can be used to more efficiently protect islet grafts from diabetogenic T cells...
  18. ncbi request reprint Immunomodulation by adenoviral-mediated SCD40-Ig gene therapy for mouse allogeneic islet transplantation
    Khaja K Rehman
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine Pittsburgh, PA 15261, USA
    Transplantation 84:301-7. 2007
    ..Local expression of an immunosuppressive agent at the site of islet transplant could promote long-term engraftment without associated systemic side effects...
  19. ncbi request reprint The insulin secretory granule is the major site of K(ATP) channels of the endocrine pancreas
    Xuehui Geng
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, 3500 Terrace Street, Pittsburgh, PA 15261, USA
    Diabetes 52:767-76. 2003
    ..Relatively little or no labeling of other structures, including the plasma membrane, was found. Our results demonstrate that the insulin secretory granule is the major site of K(ATP) channels of the endocrine pancreas...
  20. ncbi request reprint Marked prolongation of cardiac allograft survival by dendritic cells genetically engineered with NF-kappa B oligodeoxyribonucleotide decoys and adenoviral vectors encoding CTLA4-Ig
    C Andrew Bonham
    Department of Surgery and Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Immunol 169:3382-91. 2002
    ..Use of NF-kappaB antisense decoys in conjunction with rAd encoding a potent costimulation blocking agent offers promise for therapy of allograft rejection or autoimmune disease with minimization of systemic immunosuppression...
  21. pmc AAV8-mediated gene transfer of interleukin-4 to endogenous beta-cells prevents the onset of diabetes in NOD mice
    Khaja K Rehman
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Mol Ther 16:1409-16. 2008
    ..Taken together, these results demonstrate that local expression of mIL-4 in islets prevents islet destruction and blocks autoimmunity, partly through regulation of T-cell function...
  22. ncbi request reprint Intratumoral delivery of dendritic cells engineered to secrete both interleukin (IL)-12 and IL-18 effectively treats local and distant disease in association with broadly reactive Tc1-type immunity
    Tomohide Tatsumi
    Department of Surgery, University of Pittsburgh School of Medicine, The Hillman Cancer Center, 5117 Center Avenue, Pittsburgh, PA 15213, USA
    Cancer Res 63:6378-86. 2003
    ....
  23. pmc NF-κB inhibition delays DNA damage-induced senescence and aging in mice
    Jeremy S Tilstra
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    J Clin Invest 122:2601-12. 2012
    ..IKK/NF-κB inhibitors are sufficient to attenuate this damage and could provide clinical benefit for degenerative changes associated with accelerated aging disorders and normal aging...
  24. ncbi request reprint Protection of islets by in situ peptide-mediated transduction of the Ikappa B kinase inhibitor Nemo-binding domain peptide
    Khaja K Rehman
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 278:9862-8. 2003
    ..These results demonstrate the feasibility of using PTD-mediated delivery to transiently modify islets in situ to improve their viability and function during isolation, prior to transplantation...
  25. ncbi request reprint Widespread and stable pancreatic gene transfer by adeno-associated virus vectors via different routes
    Zhong Wang
    Department of Orthopedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Diabetes 55:875-84. 2006
    ..These effective new methods should provide useful tools to study diabetes pathogenesis and gene therapy...
  26. ncbi request reprint Exosomes as a short-range mechanism to spread alloantigen between dendritic cells during T cell allorecognition
    Angela Montecalvo
    Thomas E Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA 15213 2582, USA
    J Immunol 180:3081-90. 2008
    ....
  27. ncbi request reprint Identification of a synovial fibroblast-specific protein transduction domain for delivery of apoptotic agents to hyperplastic synovium
    Zhibao Mi
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    Mol Ther 8:295-305. 2003
    ..Moreover, the results demonstrate the feasibility of identifying tissue-specific transduction peptides capable of mediating efficient transduction in vivo...
  28. ncbi request reprint Gene transfer of manganese superoxide dismutase extends islet graft function in a mouse model of autoimmune diabetes
    Suzanne Bertera
    Division of Immunogenetics, Department of Pediatrics, University of Pittsburgh School of Medicine, Pennsylvania, USA
    Diabetes 52:387-93. 2003
    ..This significant prolongation of graft function suggests that the antioxidant activity of MnSOD is beneficial to transplanted islet survival and may be used in combination with other strategies aimed at islet graft protection...
  29. ncbi request reprint Local administration of IL-12-transfected dendritic cells induces antitumor immune responses to colon adenocarcinoma in the liver in mice
    Yuji Satoh
    Biologic Therapeutics Program, University of Pittsburgh Cancer Institute, 5725 CHP MT, 200 Lothrop Street, Pittsburgh, PA 15213, USA
    J Exp Ther Oncol 2:337-49. 2002
    ..Simultaneous local gene therapy with IL-12 will further improve clinical efficacy without placing the patient at risk for systemic toxicity...
  30. pmc Mitochondrial-derived reactive oxygen species (ROS) play a causal role in aging-related intervertebral disc degeneration
    Luigi A Nasto
    Ferguson Laboratory for Orthopaedic Research, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Orthop Res 31:1150-7. 2013
    ..Collectively, these data provide strong experimental evidence that mitochondrial-derived ROS play a causal role in driving changes linked to aging-related IDD and a potentially important role for radical scavengers in preventing IDD...
  31. ncbi request reprint Comparative analysis of antitumor activity of CD40L, RANKL, and 4-1BBL in vivo following intratumoral administration of viral vectors or transduced dendritic cells
    Zoya R Yurkovetsky
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    J Gene Med 8:129-37. 2006
    ..Taken together, these results demonstrate that adenoviral gene transfer of CD40L, RANKL or 4-1BBL elicit a significant antitumor effect in two different tumor models, with CD40L gene transfer inducing the strongest antitumor effect...
  32. ncbi request reprint Exosomes derived from IL-10-treated dendritic cells can suppress inflammation and collagen-induced arthritis
    Seon Hee Kim
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    J Immunol 174:6440-8. 2005
    ..Thus DC-derived exosomes may represent a novel, cell-free therapy for the treatment of autoimmune diseases...
  33. ncbi request reprint MHC class II+ exosomes in plasma suppress inflammation in an antigen-specific and Fas ligand/Fas-dependent manner
    Seon Hee Kim
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    J Immunol 179:2235-41. 2007
    ..These results suggest that exosomes in the plasma, produced by MHC class II(+) and CD11b(+) cells, have the ability to suppress the immune response in an Ag-specific manner in part through a Fas/FasL-dependent manner...
  34. pmc In vivo expression of HGF/NK1 and GLP-1 From dsAAV vectors enhances pancreatic ß-cell proliferation and improves pathology in the db/db mouse model of diabetes
    Daniel F Gaddy
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Diabetes 59:3108-16. 2010
    ..We compare the abilities of these gene products to induce islet proliferation in vitro and in vivo and characterize their abilities to regulate diabetes after AAV-mediated delivery to endogenous islets of db/db mice...
  35. pmc Therapeutic effect of exosomes from indoleamine 2,3-dioxygenase-positive dendritic cells in collagen-induced arthritis and delayed-type hypersensitivity disease models
    Nicole R Bianco
    University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania
    Arthritis Rheum 60:380-9. 2009
    ..This study was undertaken to examine the immunosuppressive effects of bone marrow (BM)-derived DCs genetically modified to express IDO, and of exosomes derived from IDO-positive DCs...
  36. pmc Genotoxic stress accelerates age-associated degenerative changes in intervertebral discs
    Luigi A Nasto
    Ferguson Laboratory for Orthopaedic Research, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Mech Ageing Dev 134:35-42. 2013
    ..Local irradiation of the vertebra in Wt mice elicited a similar reduction in PG. These data demonstrate that genotoxic stress drives degenerative changes associated with IDD...
  37. ncbi request reprint Ex vivo gene delivery of IL-1Ra and soluble TNF receptor confers a distal synergistic therapeutic effect in antigen-induced arthritis
    Seon Hee Kim
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    Mol Ther 6:591-600. 2002
    ..Moreover, the results demonstrate that local, ex vivo gene therapy for arthritis could be effective in blocking pathologies within untreated, distant arthritic joints...
  38. ncbi request reprint Innate immunity mediated by the cytokine IL-1 homologue 4 (IL-1H4/IL-1F7) induces IL-12-dependent adaptive and profound antitumor immunity
    Wentao Gao
    Department of Molecular Genetics, University of Pittsburgh, PA 15219, USA
    J Immunol 170:107-13. 2003
    ..These results suggest that IL-1H4 could play an important role in the link between innate and adaptive immunity and may be useful for tumor immunotherapy...
  39. pmc Human, viral or mutant human IL-10 expressed after local adenovirus-mediated gene transfer are equally effective in ameliorating disease pathology in a rabbit knee model of antigen-induced arthritis
    Annahita Keravala
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh, School of Medicine, Pittsburgh, PA 15261, USA
    Arthritis Res Ther 8:R91. 2006
    ..Our results suggest that hIL-10, vIL-10, and mut.hIL-10 are all equally therapeutic in the rabbit AIA model for treating disease pathology...
  40. ncbi request reprint Hyperactive Himar1 transposase mediates transposition in cell culture and enhances gene expression in vivo
    Annahita Keravala
    Department of Molecular Genetics and Biochemistry, School of Medicine, University of Pittsburgh, PA 15261, USA
    Hum Gene Ther 17:1006-18. 2006
    ..These data suggest that vectors based on the Himar1 transposable element, in conjunction with the hyperactive mutant transposase C9, may be suitable vectors for gene therapy applications...
  41. ncbi request reprint Advantages and limitations of particle-mediated transfection (gene gun) in cancer immuno-gene therapy using IL-10, IL-12 or B7-1 in murine tumor models
    Toru Kitagawa
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    J Gene Med 5:958-65. 2003
    ..We have examined the advantages and limitations of PMT in cancer immuno-gene therapy using IL-10, IL-12 or B7-1, all of which have been shown to be effective in murine tumor models using retroviral vectors...
  42. pmc Safety assessment of intradiscal gene therapy II: effect of dosing and vector choice
    Eric A Levicoff
    Department of Orthopaedic Surgery, Ferguson Laboratory for Orthopaedic Research, University of Pittsburgh Medical Center, Pittsburgh, PA, USA
    Spine (Phila Pa 1976) 33:1509-16; discussion 1517. 2008
    ....
  43. pmc NF-κB negatively impacts the myogenic potential of muscle-derived stem cells
    Aiping Lu
    Stem Cell Research Center, School of Medicine and Department of Orthopedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, USA
    Mol Ther 20:661-8. 2012
    ..Moreover, our results suggest that the improved muscle regeneration observed following inhibition of IKK/NF-κB, is mediated, at least in part, through enhanced stem cell proliferation and myogenic potential...
  44. ncbi request reprint Effective treatment of established mouse collagen-induced arthritis by systemic administration of dendritic cells genetically modified to express FasL
    Seon Hee Kim
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    Mol Ther 6:584-90. 2002
    ..These results demonstrate that systemic administration of DC/FasL is able to suppress collagen-reactive T cells, resulting in effective and sustained treatment of established CIA...
  45. ncbi request reprint Natural killer, but not natural killer T, cells play a necessary role in the promotion of an innate antitumor response induced by IL-18
    Wataru Hashimoto
    Department of Surgery, University of Pittsburgh Cancer Institute, Pittsburgh, PA, USA
    Int J Cancer 103:508-13. 2003
    ..These results are consistent with the notion that NK cells, but not NKT cells, are the major effectors in IL-18-induced innate immunity...
  46. pmc Protection of mice and poultry from lethal H5N1 avian influenza virus through adenovirus-based immunization
    Wentao Gao
    Department of Surgery and Medicine, University of Pittsburgh School of Medicine, Molecular Medicine Institute, Suite 412, 300 Technology Drive, Pittsburgh, PA 15219, USA
    J Virol 80:1959-64. 2006
    ....
  47. ncbi request reprint Comparative analysis of dendritic cells transduced with different anti-apoptotic molecules: sensitivity to tumor-induced apoptosis
    Levent Balkir
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Gene Med 6:537-44. 2004
    ..The aim of this study was to identify extrinsic and intrinsic tumor-induced apoptotic pathways in DC by targeting different anti-apoptotic molecules, including FLIP, XIAP/hILP, dominant-negative procaspase-9 and HSP70...
  48. pmc Amelioration of chronic murine colitis by peptide-mediated transduction of the IkappaB kinase inhibitor NEMO binding domain peptide
    SHAIVAL H DAVE
    Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    J Immunol 179:7852-9. 2007
    ..These results demonstrate that inhibitors of IKK, in particular a PTD-NBD peptide, may be therapeutic in the treatment of inflammatory bowel disease...
  49. pmc ISSLS prize winner: inhibition of NF-κB activity ameliorates age-associated disc degeneration in a mouse model of accelerated aging
    Luigi A Nasto
    Ferguson Laboratory for Orthopaedic Research, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Spine (Phila Pa 1976) 37:1819-25. 2012
    ..NF-κB activity was pharmacologically and genetically blocked in an accelerated aging mouse model to mitigate age-related disc degenerative changes...
  50. pmc An overview of underlying causes and animal models for the study of age-related degenerative disorders of the spine and synovial joints
    Nam Vo
    Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, 200 Lothrop Street, E1641 Biomedical Science Tower, Pittsburgh, Pennsylvania 15261, USA
    J Orthop Res 31:831-7. 2013
    ....
  51. ncbi request reprint Broad cellular immunity with robust memory responses to simian immunodeficiency virus following serial vaccination with adenovirus 5- and 35-based vectors
    Simon M Barratt-Boyes
    Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, PA 15261, USA
    J Gen Virol 87:139-49. 2006
    ..These findings support the further development of Ad35 as a vaccine vector, and promote vaccine regimens that utilize serial administration of heterologous adenoviruses...
  52. pmc Gene transfer to human joints: progress toward a gene therapy of arthritis
    Christopher H Evans
    Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Proc Natl Acad Sci U S A 102:8698-703. 2005
    ..This conclusion justifies additional efficacy studies and encourages further development of genetic approaches to the treatment of arthritis and related disorders...
  53. ncbi request reprint UpGene: Application of a web-based DNA codon optimization algorithm
    Wentao Gao
    Department of Molecular Genetics and Biochemistry and Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Biotechnol Prog 20:443-8. 2004
    ..Moreover, it can be used to optimize any other genes of interest and is freely available online at http://www.vectorcore.pitt.edu/upgene.html...
  54. pmc Isolation of muscle-derived stem/progenitor cells based on adhesion characteristics to collagen-coated surfaces
    Mitra Lavasani
    Department of Orthopaedic Surgery, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA
    Methods Mol Biol 976:53-65. 2013
    ..Here we detail methods for isolation and characterization of MDSPCs...
  55. pmc Muscle-derived stem/progenitor cell dysfunction limits healthspan and lifespan in a murine progeria model
    Mitra Lavasani
    Stem Cell Research Center, 206 Bridgeside Point II, 450 Technology Drive, Pittsburgh, Pennsylvania 15219, USA
    Nat Commun 3:608. 2012
    ..These results establish that adult stem/progenitor cell dysfunction contributes to ageing-related degeneration and suggests a therapeutic potential of post-natal stem cells to extend health...
  56. pmc Intra-articular injection of recombinant TRAIL induces synovial apoptosis and reduces inflammation in a rabbit knee model of arthritis
    Qingping Yao
    Department of Molecular Genetics and Biochemistry, 200 Lothrop Street, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Arthritis Res Ther 8:R16. 2006
    ..These results demonstrate that intra-articular injection of rTRAIL could be therapeutic for treating pathologies associated with rheumatoid arthritis...
  57. ncbi request reprint Evaluation of peptide-mediated transduction in human CD34+ cells
    Hongmei Shen
    University of Pittsburgh Cancer Institute, Department of Radiation Oncology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261
    Hum Gene Ther 15:415-9. 2004
    ..Together, these data suggest the potential usefulness of arginine homopolymers in hematopoietic stem and progenitor cell manipulations...
  58. pmc Chaperone displacement from mutant cystic fibrosis transmembrane conductance regulator restores its function in human airway epithelia
    Fei Sun
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    FASEB J 22:3255-63. 2008
    ..Thus, PTD-mediated DeltaFRD fragment delivery may provide a therapy for CF...
  59. pmc Adenoviral-mediated transfer of TGF-beta1 but not IGF-1 induces chondrogenic differentiation of human mesenchymal stem cells in pellet cultures
    Koichiro Kawamura
    Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA, USA
    Exp Hematol 33:865-72. 2005
    ..The growth factor genes evaluated in the present study were transforming growth factor 1 (TGF-beta1) and insulin-like growth factor 1 (IGF-1)...
  60. ncbi request reprint Cationic and tissue-specific protein transduction domains identification, characterization, and therapeutic application
    Maliha Zahid
    Department of Microbiology and Molecular Genetics, 450 Technology Drive, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Adv Genet 69:83-95. 2010
    ..In particular, we describe the use of screening peptide phage display libraries to identify tissue-specific transduction peptides...
  61. pmc Premature aging-related peripheral neuropathy in a mouse model of progeria
    James R Goss
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, PA 15213 1863, USA
    Mech Ageing Dev 132:437-42. 2011
    ..This provides strong evidence that DNA damage can drive peripheral neuropathy and offers a rapid and novel model to test therapies...
  62. ncbi request reprint Modulation of the immune response using dendritic cell-derived exosomes
    Nicole R Bianco
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Methods Mol Biol 380:443-55. 2007
    ..Finally, we describe methods to assess the function of exosomes in vitro, using the mixed leukocytes reaction, and in vivo by means of DTH and an experimental model of CIA...
  63. ncbi request reprint Altered trafficking of Fas and subsequent resistance to Fas-mediated apoptosis occurs by a wild-type p53 independent mechanism in esophageal adenocarcinoma
    Raja S Mahidhara
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    J Surg Res 123:302-11. 2005
    ..Because p53 is known to increase transcription of Fas and also may induce trafficking of the protein to the plasma membrane, we investigated whether the loss of wild-type (wt)-p53 function accounts for our previous findings...
  64. pmc DNA damage drives accelerated bone aging via an NF-κB-dependent mechanism
    Qian Chen
    Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    J Bone Miner Res 28:1214-28. 2013
    ..These results demonstrate that DNA damage drives osteoporosis through an NF-κB-dependent mechanism. Therefore, the NF-κB pathway represents a novel therapeutic target to treat aging-related bone disease...
  65. ncbi request reprint Immunosuppressive effects of glucosamine
    Linlin Ma
    Department of Surgery, T E Starzl Transplantation Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 277:39343-9. 2002
    ..We conclude that, despite its documented effects on insulin sensitivity, glucosamine possesses immunosuppressive activity and could be beneficial as an immunosuppressive agent...
  66. pmc Plasma-derived MHC class II+ exosomes from tumor-bearing mice suppress tumor antigen-specific immune responses
    Chenjie Yang
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA
    Eur J Immunol 42:1778-84. 2012
    ..These results demonstrate that circulating host-derived, MHC class II(+) exosomes in tumor-bearing hosts are able to suppress the immune response specific to tumor antigens...
  67. ncbi request reprint Gene transfer to the tendon-bone insertion site
    Christian Lattermann
    Department of Orthopaedic Surgery, University of Pittsburgh Medical Center, 3471 Fifth Avenue, Pittsburgh, PA 15213, USA
    Knee Surg Sports Traumatol Arthrosc 12:510-5. 2004
    ..This study has shown the feasibility of gene delivery to the tendon-bone interface and provides the basis for the application adenoviral delivery of growth factor genes to the tendon-bone insertion site...
  68. pmc Prevalence of neutralizing antibodies to adenoviral serotypes 5 and 35 in the adult populations of The Gambia, South Africa, and the United States
    Edward Nwanegbo
    Department of Surgery, University of Pittsburgh School of Medicine, Pennsylvania 15219, USA
    Clin Diagn Lab Immunol 11:351-7. 2004
    ..Because of the ability of adenoviruses to elicit systemic and mucosal immune responses, Ad35 with its low NAb prevalence appears to be an attractive candidate vector for gene therapy applications...
  69. pmc PTD-mediated loading of tumor-seeking lymphocytes with prodrug-activating enzymes
    Qin Yang
    Department of Immunology, University of Pittsburgh School of Medicine, University of Pittsburgh Cancer Institute, University of Pittsburgh, 5150 Center Avenue, Suite 1A 106, Pittsburgh, Pennsylvania 15232, USA
    AAPS J 10:614-21. 2008
    ..Ongoing studies in our laboratory are evaluating the antitumor efficacy of cellular-dependent enzyme prodrug therapy...
  70. ncbi request reprint Protein transduction domains: applications for molecular medicine
    Maliha Zahid
    Research Associate, Dept of Developmental Biology, University of Pittsburgh, PA, USA
    Curr Gene Ther 12:374-80. 2012
    ..Here we review the potential applications of protein transduction domains...
  71. ncbi request reprint Effects of a SARS-associated coronavirus vaccine in monkeys
    Wentao Gao
    Department of Surgery Molecular, Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA
    Lancet 362:1895-6. 2003
    ..These results show that an adenoviral-based vaccine can induce strong SARS-CoV-specific immune responses in the monkey, and hold promise for development of a protective vaccine against the SARS causal agent...
  72. ncbi request reprint Gene therapy strategies to prevent autoimmune disorders
    Massimo Trucco
    Department of Pediatrics, Division of Immunogenetics, Children s Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Curr Gene Ther 2:341-54. 2002
    ..The potential utility of gene therapy strategies promoting tolerance in two model autoimmune disorders, type I diabetes mellitus and rheumatoid arthritis are discussed in this review...
  73. ncbi request reprint Interleukin 18 gene transfer expands the repertoire of antitumor Th1-type immunity elicited by dendritic cell-based vaccines in association with enhanced therapeutic efficacy
    Tomohide Tatsumi
    Department of Surgery, University of Pittsburgh School of Medicine, Pennsylvania 15261, USA
    Cancer Res 62:5853-8. 2002
    ..These results support the ability of IL-18 gene transfer to enhance the capacity of DCs to drive broadly reactive Th1-type therapeutic immunity prompted by single peptide epitope-based vaccines (i.e., epitope spreading)...
  74. ncbi request reprint Peroxidation and externalization of phosphatidylserine associated with release of cytochrome c from mitochondria
    Jianfei Jiang
    Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Free Radic Biol Med 35:814-25. 2003
    ....
  75. pmc Targeted inhibition of Stat3 with a decoy oligonucleotide abrogates head and neck cancer cell growth
    Paul L Leong
    Department of Otolaryngology, University of Pittsburgh School of Medicine and University of Pittsburgh Cancer Institute, Pittsburgh, PA 15260, USA
    Proc Natl Acad Sci U S A 100:4138-43. 2003
    ..Thus, disruption of activated Stat3 by using a transcription factor decoy approach may serve as a novel therapeutic strategy for cancers characterized by constitutive Stat3 activation...
  76. ncbi request reprint Gene and cell therapies for diabetes mellitus: strategies and clinical potential
    Nick Giannoukakis
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    BioDrugs 16:149-73. 2002
    ..In the end, progress in vector design, elucidation of antigen-specific immunity and insulin sensitivity will provide the framework for gene drug use in the treatment of type 1 and type 2 diabetes...
  77. ncbi request reprint Expression of a soluble transforming growth factor-beta (TGFbeta) receptor reduces tumorigenicity by regulating natural killer (NK) cell activity against 9L gliosarcoma in vivo
    Timothy F Witham
    Brain Tumor Center, University of Pittsburgh Cancer Institute, Pittsburgh, PA 15213 1863, USA
    J Neurooncol 64:63-9. 2003
    ..Finally, we determined that elimination of CD161+ cells resulted in comparable growth of 9L-neo and 9L-TGFbeta sr in vivo, indicating that NK or NK-like cells were responsible for the anti-tumor effects in this model...
  78. pmc Accelerated aging of intervertebral discs in a mouse model of progeria
    Nam Vo
    Ferguson Laboratory for Orthopaedic Research, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Orthop Res 28:1600-7. 2010
    ..These results demonstrate that Ercc1(-/Δ) mice represent an accurate and rapid model of disc aging and provide novel evidence that DNA damage negatively impacts PG synthesis...
  79. ncbi request reprint Approaches for skeletal gene therapy
    Christopher Niyibizi
    Department of Orthopaedic Surgery, Ferguson Laboratories for Orthopaedic Research, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Crit Rev Eukaryot Gene Expr 12:163-73. 2002
    ..The challenges that the investigators face in their quest for the skeletal gene therapy are also discussed...
  80. pmc Tumor-derived exosomes confer antigen-specific immunosuppression in a murine delayed-type hypersensitivity model
    Chenjie Yang
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 6:e22517. 2011
    ..Taken together, our results suggest a role for tumor-derived exosomes in inducing tumor antigen-specific immunosuppression, possibly by modulating the function of APCs...
  81. pmc Signaling mechanisms involved in the response to genotoxic stress and regulating lifespan
    Laura J Niedernhofer
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, University of Pittsburgh Cancer Institute, PA 15213, USA
    Int J Biochem Cell Biol 40:176-80. 2008
    ..This review highlights the significant overlap in signaling mechanisms implicated in both the cellular response to genotoxic stress and regulation of organism lifespan...
  82. doi request reprint Identification and characterization of tissue-specific protein transduction domains using peptide phage display
    Maliha Zahid
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Biomedical Science Tower, Pittsburgh, PA, USA
    Methods Mol Biol 683:277-89. 2011
    ..In this chapter, we described methods for screening peptide phage display libraries for cell and tissue-specific transduction peptides both in cell culture and in vivo and for functional analysis of transduction...
  83. ncbi request reprint Expression and activity of the CDK inhibitor p57Kip2 in chondrocytes undergoing hypertrophic differentiation
    Matthew C Stewart
    Department of Veterinary Clinical Medicine, University of Illinois at Urbana Champaign, Urbana, Illinois 61802, USA
    J Bone Miner Res 19:123-32. 2004
    ..The phenotypic consequences of p57 expression were assessed in an in vitro model of hypertrophic differentiation. Adenoviral p57 expression was not sufficient for CollX expression but did augment induction of CollX by BMP-2...
  84. ncbi request reprint Human periprosthetic tissues implanted in severe combined immunodeficient mice respond to gene transfer of a cytokine inhibitor
    Shang You Yang
    Department of Orthopaedic Surgery, Wayne State University, University Health Center 7C, 4201 St Antoine Boulevard, Detroit, Michigan 48201, USA
    J Bone Joint Surg Am 87:1088-97. 2005
    ..This study evaluated a retrovirus-mediated gene therapy with use of a novel xenograft-based animal model...
  85. ncbi request reprint Current status of gene therapy for rheumatoid arthritis
    Daniel F Gaddy
    Curr Rheumatol Rep 10:398-404. 2008
    ..In this review, we explore the advances in gene therapy for RA and summarize the recent preclinical and clinical data. In addition, we provide an overview of vectors and targets for RA gene therapy...
  86. ncbi request reprint Increased function and survival of IL-15-transduced human dendritic cells are mediated by up-regulation of IL-15Ralpha and Bcl-2
    Irina L Tourkova
    Department of Pathology, University of Pittsburgh Medical Center and University of Pittsburgh Cancer Institute, Pennsylvania 15213, USA
    J Leukoc Biol 72:1037-45. 2002
    ..In summary, these results suggest that transduction of DC with the IL-15 gene markedly stimulates DC function and protects them from tumor-induced apoptosis...
  87. ncbi request reprint Gene therapy for the treatment of musculoskeletal diseases
    Christopher H Evans
    Center for Molecular Orthopaedics, Department of Orthopaedic Surgery, Harvard Medical School, Boston, MA 02115, USA
    J Am Acad Orthop Surg 13:230-42. 2005
    ..These developments have the potential to transform many areas of musculoskeletal care, leading to treatments that are less invasive, more effective, and less expensive than existing modalities...
  88. ncbi request reprint IL-1ra/IGF-1 gene therapy modulates repair of microfractured chondral defects
    Sophie Morisset
    Orthopaedic Research Center, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80524, USA
    Clin Orthop Relat Res 462:221-8. 2007
    ..These data suggest in vivo gene therapy can improve biologic processes associated with chondral defect repair...
  89. ncbi request reprint Indoleamine 2,3-dioxygenase expression in transplanted NOD Islets prolongs graft survival after adoptive transfer of diabetogenic splenocytes
    Angela M Alexander
    Division of Immunogenetics, Department of Pediatrics, Rangos Research Center, Children s Hospital of Pittsburgh, University of Pittsburgh, School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Diabetes 51:356-65. 2002
    ..These results suggest that local modulation of tryptophan catabolism may be a means of facilitating islet transplantation as a therapy for type 1 diabetes...
  90. ncbi request reprint PhiC31 integrase mediates integration in cultured synovial cells and enhances gene expression in rabbit joints
    Annahita Keravala
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305 5120, USA
    J Gene Med 8:1008-17. 2006
    ..We report a novel approach for non-viral gene therapy to joints that utilizes phage phiC31 integrase to bring about unidirectional genomic integration...
  91. ncbi request reprint Stroma formation and angiogenesis by overexpression of growth factors, cytokines, and proteolytic enzymes in human skin grafted to SCID mice
    Claus J Gruss
    The Wistar Institute, Philadelphia, PA Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute, Leuven, Belgium
    J Invest Dermatol 120:683-92. 2003
    ..The overall results suggest that adenoviral vectors can effectively remodel the architecture of human skin for studies in morphogenesis, inflammatory skin disorders, wound healing, and cancer development...
  92. ncbi request reprint Safety assessment of intradiscal gene transfer: a pilot study
    Corey J Wallach
    Ferguson Laboratory for Orthopaedic Research, Department of Orthopaedic Surgery, University of Pittsburgh Medical Center, 200 Lothrop Street, C313 PUH, PA 15213, USA
    Spine J 6:107-12. 2006
    ....
  93. ncbi request reprint Differential effects of IL-12 on the generation of alloreactive CTL mediated by murine and human dendritic cells: a critical role for nitric oxide
    Yasuhiko Nishioka
    Third Department of Internal Medicine, University of Tokushima School of Medicine, Japan
    J Leukoc Biol 73:621-9. 2003
    ..These results suggest that NO produced by DCs might play an important role in IL-12-mediated immune suppression in mice but not in humans...
  94. pmc The 3rd International Meeting on Gene Therapy in Rheumatology and Orthopaedics
    Christopher H Evans
    Center for Molecular Orthopaedics, Harvard Medical School, Boston, Massachusetts, USA
    Arthritis Res Ther 7:273-8. 2005
    ..Applications in mesenchymal stem cell biology, tissue repair, and regenerative medicine were also addressed. The field has advanced considerably since the previous meeting in this series, and further clinical trials seem likely...
  95. ncbi request reprint The fate of mesenchymal stem cells transplanted into immunocompetent neonatal mice: implications for skeletal gene therapy via stem cells
    Christopher Niyibizi
    Department of Orthopaedics and Rehabilitation, Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA
    Mol Ther 9:955-63. 2004
    ..These data demonstrate that MSCs persist in immunocompetent neonatal mice, maintain a high level of gene expression, and may participate in skeletal growth and development of the recipient animals...
  96. ncbi request reprint Dual transduction of insulin-like growth factor-I and interleukin-1 receptor antagonist protein controls cartilage degradation in an osteoarthritic culture model
    Jennifer L Haupt
    Comparative Orthopaedics Laboratory, College of Veterinary Medicine, Cornell University, C3 176 Veterinary Medical Center, Ithaca, NY 14853, USA
    J Orthop Res 23:118-26. 2005
    ....
  97. ncbi request reprint The adeno associated viral vector as a strategy for intradiscal gene transfer in immune competent and pre-exposed rabbits
    Christian Lattermann
    Department of Orthopaedic Surgery, University of Pittsburgh Medical Center, PA, USA
    Spine (Phila Pa 1976) 30:497-504. 2005
    ..Experimental animal study...
  98. ncbi request reprint Perspectives on the use of gene therapy for chronic joint diseases
    Steven C Ghivizzani
    Gene Therapy Laboratory, Department of Orthopaedics and Rehabilitation, University of Florida College of Medicine, Gainesville FL 32610, USA
    Curr Gene Ther 8:273-86. 2008
    ..This review attempts to condense these studies and to discuss the relative benefits and limitations of the methods proposed and to discuss the challenges toward translating these technologies into clinical realities...
  99. ncbi request reprint Interleukin-17 promotes angiogenesis and tumor growth
    Muneo Numasaki
    Department of Surgery, School of Medicine, University of Pittsburgh Cancer Institute, University of Pittsburgh, PA, USA
    Blood 101:2620-7. 2003
    ..Furthermore, they suggest that inhibition of biologic action of IL-17 may have therapeutic benefits when applied to angiogenesis-related disorders...
  100. ncbi request reprint Development of gene-based therapies for cartilage repair
    Glyn Palmer
    Center for Molecular Orthopaedics, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Crit Rev Eukaryot Gene Expr 12:259-73. 2002
    ..This article reviews the current status of gene delivery for cartilage healing and presents some of the remaining challenges...

Research Grants17

  1. LMP and Bone Healing
    Paul Robbins; Fiscal Year: 2009
    ....
  2. Inhibition of NF-KB to Facilitate Islet Transplantation
    Paul Robbins; Fiscal Year: 2003
    ..The successful completion of the proposed studies should determine if NF-kB inhibition in islets by peptide and gene mediated transduction is able to improve islet viability prior to and post-transplantation. ..
  3. REGULATION OF TRANSCRIPTION BY THE RB ANTIONCOGENE
    Paul Robbins; Fiscal Year: 2002
    ..The experiments described in the proposal are expected to provide important insights into the mechanism of how RB and cyclin D1 function to regulate transcription directly, cell differentiation, and tumorigenesis. ..
  4. REGULATION OF TRANSCRIPTION BY THE RB ANTIONCOGENE
    Paul Robbins; Fiscal Year: 2001
    ..The experiments described in the proposal are expected to provide important insights into the mechanism of how RB and cyclin D1 function to regulate transcription directly, cell differentiation, and tumorigenesis. ..
  5. REGULATION OF TRANSCRIPTION BY THE RB ANTIONCOGENE
    Paul Robbins; Fiscal Year: 2000
    ..The experiments described in the proposal are expected to provide important insights into the mechanism of how RB and cyclin D1 function to regulate transcription directly, cell differentiation, and tumorigenesis. ..
  6. REGULATION OF TRANSCRIPTION-RETINOBLASTOMA ANTI-ONCOGENE
    Paul Robbins; Fiscal Year: 1993
    ..The successful completion of the project should significantly increase our understanding of the mechanism of action of an anti-oncogene and could potentially lead to a biological treatment for certain cancers...
  7. REGULATION OF TRANSCRIPTION-RETINOBLASTOMA ANTI-ONCOGENE
    Paul Robbins; Fiscal Year: 1992
    ..The successful completion of the project should significantly increase our understanding of the mechanism of action of an anti-oncogene and could potentially lead to a biological treatment for certain cancers...
  8. REGULATION OF TRANSCRIPTION-RETINOBLASTOMA ANTI-ONCOGENE
    Paul Robbins; Fiscal Year: 1991
    ..The successful completion of the project should significantly increase our understanding of the mechanism of action of an anti-oncogene and could potentially lead to a biological treatment for certain cancers...
  9. REGULATION OF TRANSCRIPTION BY THE RB ANTIONCOGENE
    Paul Robbins; Fiscal Year: 1999
    ..The experiments described in the proposal are expected to provide important insights into the mechanism of how RB and cyclin D1 function to regulate transcription directly, cell differentiation, and tumorigenesis. ..
  10. LMP and Bone Healing
    Paul D Robbins; Fiscal Year: 2010
    ....