Josep Rizo

Summary

Affiliation: University of Texas Southwestern Medical Center
Country: USA

Publications

  1. ncbi request reprint Phosphatidylinositol phosphates as co-activators of Ca2+ binding to C2 domains of synaptotagmin 1
    LiYi Li
    Department of Neuroscience, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 281:15845-52. 2006
  2. ncbi request reprint Unraveling the mechanisms of synaptotagmin and SNARE function in neurotransmitter release
    Josep Rizo
    Departments of Biochemistry and Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Trends Cell Biol 16:339-50. 2006
  3. pmc Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch
    Jun Lu
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    PLoS Biol 4:e192. 2006
  4. pmc Munc13 mediates the transition from the closed syntaxin-Munc18 complex to the SNARE complex
    Cong Ma
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    Nat Struct Mol Biol 18:542-9. 2011
  5. pmc Membrane bridging and hemifusion by denaturated Munc18
    Yi Xu
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS ONE 6:e22012. 2011
  6. pmc Munc13 C2B domain is an activity-dependent Ca2+ regulator of synaptic exocytosis
    Ok Ho Shin
    Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    Nat Struct Mol Biol 17:280-8. 2010
  7. doi request reprint Synaptic vesicle fusion without SNARE transmembrane regions
    Josep Rizo
    Departments of Biophysics, Biochemistry, and Pharmacology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA Electronic address
    Dev Cell 27:124-6. 2013
  8. pmc Enlightening molecular mechanisms through study of protein interactions
    Josep Rizo
    Department of Biophysics, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA
    J Mol Cell Biol 4:270-83. 2012
  9. doi request reprint The membrane fusion enigma: SNAREs, Sec1/Munc18 proteins, and their accomplices--guilty as charged?
    Josep Rizo
    Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Annu Rev Cell Dev Biol 28:279-308. 2012
  10. ncbi request reprint Snares and Munc18 in synaptic vesicle fusion
    Josep Rizo
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Nat Rev Neurosci 3:641-53. 2002

Research Grants

Collaborators

Detail Information

Publications76

  1. ncbi request reprint Phosphatidylinositol phosphates as co-activators of Ca2+ binding to C2 domains of synaptotagmin 1
    LiYi Li
    Department of Neuroscience, Baylor College of Medicine, Houston, Texas 77030, USA
    J Biol Chem 281:15845-52. 2006
    ....
  2. ncbi request reprint Unraveling the mechanisms of synaptotagmin and SNARE function in neurotransmitter release
    Josep Rizo
    Departments of Biochemistry and Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Trends Cell Biol 16:339-50. 2006
    ..Understanding the functions of these proteins will be crucial to reach a faithful description of the mechanisms of membrane fusion and neurotransmitter release...
  3. pmc Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch
    Jun Lu
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    PLoS Biol 4:e192. 2006
    ....
  4. pmc Munc13 mediates the transition from the closed syntaxin-Munc18 complex to the SNARE complex
    Cong Ma
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    Nat Struct Mol Biol 18:542-9. 2011
    ....
  5. pmc Membrane bridging and hemifusion by denaturated Munc18
    Yi Xu
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS ONE 6:e22012. 2011
    ..In addition, our data suggest a novel mechanism of membrane hemifusion induced by amphipathic macromolecules that does not involve formation of a stalk intermediate...
  6. pmc Munc13 C2B domain is an activity-dependent Ca2+ regulator of synaptic exocytosis
    Ok Ho Shin
    Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    Nat Struct Mol Biol 17:280-8. 2010
    ....
  7. doi request reprint Synaptic vesicle fusion without SNARE transmembrane regions
    Josep Rizo
    Departments of Biophysics, Biochemistry, and Pharmacology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA Electronic address
    Dev Cell 27:124-6. 2013
    ..Reporting in Neuron, Zhou et al. (2013) show that lipid-anchored SNAREs lacking TMRs can support neurotransmitter release, suggesting that SNAREs function primarily as power engines that force membranes together. ..
  8. pmc Enlightening molecular mechanisms through study of protein interactions
    Josep Rizo
    Department of Biophysics, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA
    J Mol Cell Biol 4:270-83. 2012
    ..Overall, this research underlines the complexities involved in elucidating molecular mechanisms and how these mechanisms can depend critically on an interplay between strong and weak protein interactions...
  9. doi request reprint The membrane fusion enigma: SNAREs, Sec1/Munc18 proteins, and their accomplices--guilty as charged?
    Josep Rizo
    Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Annu Rev Cell Dev Biol 28:279-308. 2012
    ..MUN domain activity is likely modulated in diverse presynaptic plasticity processes that depend on Ca(2+) and RIM proteins, among others...
  10. ncbi request reprint Snares and Munc18 in synaptic vesicle fusion
    Josep Rizo
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Nat Rev Neurosci 3:641-53. 2002
  11. pmc Synaptic vesicle fusion
    Josep Rizo
    Department of Biochemistry, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Nat Struct Mol Biol 15:665-74. 2008
    ..Elucidation of the release mechanism will require a full understanding of the network of interactions among all these proteins and the membranes...
  12. pmc Illuminating membrane fusion
    Josep Rizo
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 103:19611-2. 2006
  13. pmc Crystal structure of the RIM1alpha C2B domain at 1.7 A resolution
    Rong Guan
    Department of Biochemistry, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Biochemistry 46:8988-98. 2007
    ..Our results provide a structural basis to understand the function of RIM C2B domains and suggest that dimerization may be a crucial aspect of RIM function...
  14. pmc Munc18-1 binding to the neuronal SNARE complex controls synaptic vesicle priming
    Ferenc Deak
    Howard Hughes Medical Institute, Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Cell Biol 184:751-64. 2009
    ....
  15. pmc Insights into mad2 regulation in the spindle checkpoint revealed by the crystal structure of the symmetric mad2 dimer
    Maojun Yang
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS Biol 6:e50. 2008
    ..Collectively, our results establish the existence of a symmetric Mad2 dimer and provide insights into Mad1-assisted conformational activation of Mad2 in the spindle checkpoint...
  16. ncbi request reprint Distinct domains of complexin I differentially regulate neurotransmitter release
    Mingshan Xue
    Department of Neuroscience, Baylor College of Medicine, Houston, Texas 77030, USA
    Nat Struct Mol Biol 14:949-58. 2007
    ....
  17. ncbi request reprint Structural basis for the evolutionary inactivation of Ca2+ binding to synaptotagmin 4
    Han Dai
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Nat Struct Mol Biol 11:844-9. 2004
    ....
  18. pmc Convergence and divergence in the mechanism of SNARE binding by Sec1/Munc18-like proteins
    Irina Dulubova
    Department of Biochemistry, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas 75390, USA
    Proc Natl Acad Sci U S A 100:32-7. 2003
    ....
  19. ncbi request reprint Structure/function analysis of Ca2+ binding to the C2A domain of synaptotagmin 1
    Rafael Fernandez-Chacon
    Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, Dallas, Texas 75390 9111, USA
    J Neurosci 22:8438-46. 2002
    ..Our data also demonstrate that subtle changes in the biochemical properties of synaptotagmin 1 can result in significant alterations in synaptic responses...
  20. pmc p31comet blocks Mad2 activation through structural mimicry
    Maojun Yang
    Department of Pharmacology, The University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
    Cell 131:744-55. 2007
    ..p31(comet) adopts a fold strikingly similar to that of Mad2 and binds at the dimerization interface of Mad2. Thus, p31(comet) exploits the two-state behavior of Mad2 to block its activation by acting as an "anti-Mad2."..
  21. ncbi request reprint A complexin/synaptotagmin 1 switch controls fast synaptic vesicle exocytosis
    Jiong Tang
    The Center for Basic Neuroscience, UT Southwestern Medical Center, Dallas, TX 75390, USA
    Cell 126:1175-87. 2006
    ....
  22. pmc Conformation-specific binding of p31(comet) antagonizes the function of Mad2 in the spindle checkpoint
    Guohong Xia
    Department of Pharmacology, The University of Texas, Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    EMBO J 23:3133-43. 2004
    ..Therefore, our results suggest that p31(comet) counteracts the function of Mad2 and is required for the silencing of the spindle checkpoint...
  23. ncbi request reprint A minimal domain responsible for Munc13 activity
    Jayeeta Basu
    Department of Neuroscience, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Nat Struct Mol Biol 12:1017-8. 2005
    ..Here we identify a large alpha-helical domain of mammalian Munc13-1 that is autonomously folded and is sufficient to rescue the total arrest in neurotransmitter release observed in hippocampal neurons lacking Munc13s...
  24. ncbi request reprint Solution structure of the Vam7p PX domain
    Jun Lu
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Biochemistry 41:5956-62. 2002
    ....
  25. pmc Remote homology between Munc13 MUN domain and vesicle tethering complexes
    Jimin Pei
    Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Mol Biol 391:509-17. 2009
    ....
  26. pmc Conformational switch of syntaxin-1 controls synaptic vesicle fusion
    Stefan H Gerber
    Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390 9111, USA
    Science 321:1507-10. 2008
    ..Thus, the closed conformation of syntaxin-1 gates the initiation of the synaptic vesicle fusion reaction, which is then mediated by SNARE-complex/Munc18-1 assemblies...
  27. ncbi request reprint Close membrane-membrane proximity induced by Ca(2+)-dependent multivalent binding of synaptotagmin-1 to phospholipids
    Demet Arac
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Nat Struct Mol Biol 13:209-17. 2006
    ..We propose a model wherein synaptotagmin cooperates with the SNAREs in bringing the synaptic vesicle and plasma membranes together and accelerates membrane fusion through the highly positive electrostatic potential of its C(2)B domain...
  28. pmc How Tlg2p/syntaxin 16 'snares' Vps45
    Irina Dulubova
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    EMBO J 21:3620-31. 2002
    ..Thus, this mechanism represents the most widespread mode of coupling between syntaxins and SM proteins...
  29. ncbi request reprint Synaptotagmin function in dense core vesicle exocytosis studied in cracked PC12 cells
    Ok Ho Shin
    Center for Basic Neuroscience, Department of Molecular Genetics and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 6000 Harry Hines Blvd, Dallas, Texas 75390, USA
    Nat Neurosci 5:649-56. 2002
    ....
  30. ncbi request reprint The Mad2 spindle checkpoint protein has two distinct natively folded states
    Xuelian Luo
    Department of Pharmacology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Nat Struct Mol Biol 11:338-45. 2004
    ..Our results suggest that the unusual two-state behavior of Mad2 is critical for spindle checkpoint signaling...
  31. pmc The Janus-faced nature of the C(2)B domain is fundamental for synaptotagmin-1 function
    Mingshan Xue
    Department of Neuroscience, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Nat Struct Mol Biol 15:1160-8. 2008
    ....
  32. pmc Binding of the complexin N terminus to the SNARE complex potentiates synaptic-vesicle fusogenicity
    Mingshan Xue
    Department of Neuroscience, Baylor College of Medicine, Houston, Texas, USA
    Nat Struct Mol Biol 17:568-75. 2010
    ....
  33. pmc The crystal structure of a Munc13 C-terminal module exhibits a remarkable similarity to vesicle tethering factors
    Wei Li
    Department of Biochemistry, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA
    Structure 19:1443-55. 2011
    ..We propose a model whereby the MUN-CD module is central for Munc13 function but full activity requires adjacent sequences...
  34. ncbi request reprint Three-dimensional structure of the complexin/SNARE complex
    Xiaocheng Chen
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Neuron 33:397-409. 2002
    ..These results suggest that complexin stabilizes the fully assembled SNARE complex as a key step that enables the exquisitely high speed of Ca(2+)-evoked neurotransmitter release...
  35. ncbi request reprint Are neuronal SNARE proteins Ca2+ sensors?
    Xiaocheng Chen
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    J Mol Biol 347:145-58. 2005
    ..These results suggest that the SNAREs do not act directly as Ca2+ receptors but SNARE complex assembly is coupled tightly to Ca2+-sensing during neurotransmitter release...
  36. pmc Genetic analysis of synaptotagmin-7 function in synaptic vesicle exocytosis
    Anton Maximov
    Departments of Neuroscience, Molecular Genetics, Pharmacology, and Biochemistry and Howard Hughes Medical Institute, UT Southwestern Medical Center, Dallas, TX 75390 9111, USA
    Proc Natl Acad Sci U S A 105:3986-91. 2008
    ....
  37. pmc A quaternary SNARE-synaptotagmin-Ca2+-phospholipid complex in neurotransmitter release
    Han Dai
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Mol Biol 367:848-63. 2007
    ..We propose a model whereby the highly positive electrostatic potential at the tip of the SSCAP complex helps to induce membrane fusion during release...
  38. ncbi request reprint A conformational switch in the Piccolo C2A domain regulated by alternative splicing
    Jesus Garcia
    Department of Biochemistry and Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Nat Struct Mol Biol 11:45-53. 2004
    ..These results reveal a novel mechanism of action of C2 domains and uncover a structural principle that may underlie the alteration of protein function by short alternatively spliced sequences...
  39. pmc Rabphilin regulates SNARE-dependent re-priming of synaptic vesicles for fusion
    Ferenc Deak
    Center for Basic Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390 9111, USA
    EMBO J 25:2856-66. 2006
    ....
  40. pmc A Munc13/RIM/Rab3 tripartite complex: from priming to plasticity?
    Irina Dulubova
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390 8816, USA
    EMBO J 24:2839-50. 2005
    ..The modular architecture of alpha-RIMs, with nested binding sites for Rab3 and other targets, may be a general feature of Rab effectors that share homology with the alpha-RIM N-terminal sequence...
  41. ncbi request reprint Three-dimensional structure of an independently folded extracellular domain of human amyloid-beta precursor protein
    Irina Dulubova
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA Irina Dulubova UTSouthwestern edu
    Biochemistry 43:9583-8. 2004
    ..The identification of this domain and the availability of its atomic structure will facilitate analysis of APP function and of the role of the extracellular region in the regulation of APP cleavage...
  42. pmc Differential but convergent functions of Ca2+ binding to synaptotagmin-1 C2 domains mediate neurotransmitter release
    Ok Ho Shin
    Departments of Neuroscience, Molecular Genetics, Biochemistry, and Pharmacology, and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390 9111, USA
    Proc Natl Acad Sci U S A 106:16469-74. 2009
    ....
  43. pmc Genetic analysis of synaptotagmin 2 in spontaneous and Ca2+-triggered neurotransmitter release
    Zhiping P Pang
    Department of Molecular Genetics, Center for Basic Neuroscience, Howard Hughes Medical Institute, UT Southwestern Medical Center, Dallas, TX 75390 9111, USA
    EMBO J 25:2039-50. 2006
    ....
  44. ncbi request reprint Solution structure of the RIM1alpha PDZ domain in complex with an ELKS1b C-terminal peptide
    Jun Lu
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390, USA
    J Mol Biol 352:455-66. 2005
    ..This groove is formed, in part, by a set of side-chains that is conserved selectively in RIM PDZ domains and that hence determines, at least in part, their unique specificity...
  45. ncbi request reprint Crystal structure of the RIM2 C2A-domain at 1.4 A resolution
    Han Dai
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Biochemistry 44:13533-42. 2005
    ....
  46. ncbi request reprint Three-dimensional structure of the rSly1 N-terminal domain reveals a conformational change induced by binding to syntaxin 5
    Demet Arac
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    J Mol Biol 346:589-601. 2005
    ....
  47. pmc Unexpected Ca2+-binding properties of synaptotagmin 9
    Ok Ho Shin
    Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, Dallas, TX 75390 9111, USA
    Proc Natl Acad Sci U S A 101:2554-9. 2004
    ..Nevertheless, the C2A domain of Syt 9 functions as a Ca2+-binding module, suggesting that Syts 1 and 9 are Ca2+ sensors with similar Ca2+-binding sequences but distinct properties that indicate nonoverlapping functions...
  48. pmc Reluctance to membrane binding enables accessibility of the synaptobrevin SNARE motif for SNARE complex formation
    Kyle D Brewer
    Departments of Biochemistry and Pharmacology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 108:12723-8. 2011
    ..We propose that the charge and hydrophobicity of SNARE motifs is optimized to enable formation of highly stable SNARE complexes while at the same time avoiding membrane binding, which could hinder SNARE complex assembly...
  49. ncbi request reprint Facile detection of protein-protein interactions by one-dimensional NMR spectroscopy
    Demet Arac
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390 9038, USA
    Biochemistry 42:2774-80. 2003
    ..A study showing that phospholipids compete with the neuronal core complex for Ca2+-dependent binding to the presynaptic Ca2+-sensor synaptotagmin 1 illustrates the usefulness of the SMRC method in studying multicomponent systems...
  50. ncbi request reprint Dual modes of Munc18-1/SNARE interactions are coupled by functionally critical binding to syntaxin-1 N terminus
    Mikhail Khvotchev
    Department of Neuroscience, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Neurosci 27:12147-55. 2007
    ..Viewed together, our data indicate that binding of Munc18 to the syntaxin N terminus unites different modes of Munc18/SNARE interactions and is essential for exocytic membrane fusion...
  51. doi request reprint Binding of the Munc13-1 MUN domain to membrane-anchored SNARE complexes
    Rong Guan
    Department of Biochemistry, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Biochemistry 47:1474-81. 2008
    ....
  52. ncbi request reprint Evidence for SNARE zippering during Ca2+-triggered exocytosis in PC12 cells
    Maria F Matos
    Center for Basic Neuroscience, Department of Molecular Genetics and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA
    Neuropharmacology 45:777-86. 2003
    ....
  53. ncbi request reprint Intramolecular occlusion of the diacylglycerol-binding site in the C1 domain of munc13-1
    Nan Shen
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Biochemistry 44:1089-96. 2005
    ....
  54. ncbi request reprint A broken alpha -helix in folded alpha -Synuclein
    Sreeganga Chandra
    Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9111, USA
    J Biol Chem 278:15313-8. 2003
    ..The structural organization of the alpha-helices of alpha-synuclein was not anticipated by sequence analyses and may be important for its pathogenic role...
  55. pmc Munc18-1 binds directly to the neuronal SNARE complex
    Irina Dulubova
    Department of Biochemistry and Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 104:2697-702. 2007
    ....
  56. ncbi request reprint Role of electrostatic and hydrophobic interactions in Ca(2+)-dependent phospholipid binding by the C(2)A-domain from synaptotagmin I
    Stefan H Gerber
    Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, Dallas, Texas, USA
    Diabetes 51:S12-8. 2002
    ..The complex phospholipid binding mode of synaptotagmins may be important for its role in regulated exocytosis of secretory granules and synaptic vesicles...
  57. ncbi request reprint Ca(2+)-binding mode of the C2A-domain of synaptotagmin
    Josep Rizo
    Departments of Biochemistry and Pharmacology, University of Texas Southwestern Medical Centre, Dallas, TX, USA
    Methods Mol Biol 172:305-16. 2002
  58. pmc Binding of Munc18-1 to synaptobrevin and to the SNARE four-helix bundle
    Yi Xu
    Department of Biochemistry, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Biochemistry 49:1568-76. 2010
    ....
  59. pmc Prevalent mechanism of membrane bridging by synaptotagmin-1
    Alpay B Seven
    Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 110:E3243-52. 2013
    ....
  60. pmc Subtle Interplay between synaptotagmin and complexin binding to the SNARE complex
    Junjie Xu
    Department of Biophysics, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA
    J Mol Biol 425:3461-75. 2013
    ....
  61. ncbi request reprint NMR structures of the selenoproteins Sep15 and SelM reveal redox activity of a new thioredoxin-like family
    Andrew D Ferguson
    Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Biol Chem 281:3536-43. 2006
    ..A physiological role for Sep15 and SelM as thiol-disulfide oxidoreductases and their contribution to the quality control pathways of the endoplasmic reticulum are discussed...
  62. ncbi request reprint Sly1 binds to Golgi and ER syntaxins via a conserved N-terminal peptide motif
    Tomohiro Yamaguchi
    Center for Basic Neuroscience, Department of Molecular Genetics, Howard Hughes Medical Institute, UT Southwestern Medical Center, Dallas, TX 75390, USA
    Dev Cell 2:295-305. 2002
    ..These data suggest a potentially general mechanism by which SM proteins could interact with peptides in target proteins independent of core complex assembly and suggest that munc18 binding to syntaxin is an exception...
  63. pmc SNARE-mediated lipid mixing depends on the physical state of the vesicles
    Xiaocheng Chen
    Department of Biochemistry and Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Biophys J 90:2062-74. 2006
    ....
  64. pmc NMR analysis of the closed conformation of syntaxin-1
    Xiaocheng Chen
    Department of Biochemistry, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA
    J Biomol NMR 41:43-54. 2008
    ....
  65. ncbi request reprint Antibacterial membrane attack by a pore-forming intestinal C-type lectin
    Sohini Mukherjee
    Department of Immunology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Nature 505:103-7. 2014
    ..Our findings identify C-type lectins as mediators of membrane attack in the mucosal immune system, and provide detailed insight into an antibacterial mechanism that promotes mutualism with the resident microbiota. ..
  66. pmc Structure and ca(2+)-binding properties of the tandem c2 domains of e-syt2
    Junjie Xu
    Department of Biophysics, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA Department of Biochemistry, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA Department of Pharmacology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA
    Structure 22:269-80. 2014
    ..These results suggest that E-Syt2 performs an as yet unidentified Ca(2+)-dependent function through its C2A domain and uncover fundamental differences between the properties of the tandem C2 domains of E-Syts and synaptotagmins. ..
  67. pmc Analysis of SNARE complex/synaptotagmin-1 interactions by one-dimensional NMR spectroscopy
    Amy Zhou
    Departments of Biophysics, Biochemistry and Pharmacology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, Texas 75390, United States
    Biochemistry 52:3446-56. 2013
    ....
  68. ncbi request reprint A protein sequence that can encode native structure by disfavoring alternate conformations
    W Christian Wigley
    Department of Physiology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390 9040, USA
    Nat Struct Biol 9:381-8. 2002
    ....
  69. ncbi request reprint Structural analysis of conserved oligomeric Golgi complex subunit 2
    Lorraine F Cavanaugh
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    J Biol Chem 282:23418-26. 2007
    ..These structures may represent platforms for interaction with other trafficking proteins including SNAREs (soluble N-ethylmaleimide factor attachment protein receptors) and Rabs...
  70. ncbi request reprint Complexin/synaptotagmin interplay controls acrosomal exocytosis
    Carlos M Roggero
    Laboratorio de Biologia Celular y Molecular, Instituto de Histologia y Embriologia IHEM CONICET, Facultad de Ciencias Medicas, Universidad Nacional de Cuyo, Mendoza 5500, Argentina
    J Biol Chem 282:26335-43. 2007
    ..Our results show that the functional interplay between complexin and synaptotagmin has a central role in a physiological secretion event, and that this interplay can be modulated by phosphorylation of the C2B domain...
  71. ncbi request reprint NMR measurement of the off rate from the first calcium-binding site of the synaptotagmin I C2A domain
    Oscar Millet
    Departament de Quimica Organica, Universitat de Barcelona, Marti i Franques 1 11, 08028, Barcelona, Spain
    FEBS Lett 516:93-6. 2002
    ..These results are consistent with the proposed role of synaptotagmin I as a calcium sensor in release, but suggest that additional factors may help to accelerate the diffusion of Ca2+ to the sensor...
  72. ncbi request reprint SNARE function revisited
    Josep Rizo
    Nat Struct Biol 10:417-9. 2003
  73. ncbi request reprint The N-terminal domains of syntaxin 7 and vti1b form three-helix bundles that differ in their ability to regulate SNARE complex assembly
    Wolfram Antonin
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    J Biol Chem 277:36449-56. 2002
    ..However, they differ in their ability to adopt closed conformations and thus to regulate the assembly of SNARE complexes...
  74. ncbi request reprint The Mad2 spindle checkpoint protein undergoes similar major conformational changes upon binding to either Mad1 or Cdc20
    Xuelian Luo
    Department of Biochemistry, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Mol Cell 9:59-71. 2002
    ..Our data suggest that, upon checkpoint activation, Mad1 recruits Mad2 to unattached kinetochores and may promote binding of Mad2 to Cdc20...
  75. ncbi request reprint How much can SNAREs flex their muscles?
    Josep Rizo
    Nat Struct Mol Biol 14:880-2. 2007
  76. ncbi request reprint Endocytosis of synaptotagmin 1 is mediated by a novel, tryptophan-containing motif
    Nadine Jarousse
    University of California San Francisco, Genentech Hall, 600 16th St, San Francisco, California 94143 2140, USA
    Traffic 4:468-78. 2003
    ..We conclude that endocytosis of synaptotagmin 1 requires a novel type of internalization signal that is subject to regulation by the rest of the C2B domain...

Research Grants25

  1. Neurotransmitter release machinery: structure and function
    JOSE RIZO REY; Fiscal Year: 2010
    ....
  2. Synaptotagmin and C2-Domains: Structure and Function
    JOSE RIZO REY; Fiscal Year: 2005
    ....
  3. Structure and Function of Syntaxin 1
    JOSE RIZO REY; Fiscal Year: 2005
    ....
  4. Synaptotagmin and C2-Domains: Structure and Function
    JOSE RIZO REY; Fiscal Year: 2006
    ....
  5. Structure and Function of Syntaxin 1
    JOSE RIZO REY; Fiscal Year: 2006
    ....
  6. Synaptotagmin and C2-Domains: Structure and Function
    JOSE RIZO REY; Fiscal Year: 2007
    ....
  7. Neurotransmitter release machinery: structure and function
    JOSE RIZO REY; Fiscal Year: 2007
    ....
  8. Synaptotagmin and C2-Domains: Structure and Function
    JOSE RIZO REY; Fiscal Year: 2009
    ..Moreover, since many neurological disorders are treated with drugs that alter synaptic transmission, this research is expected to provide crucial clues for the development of novel strategies to understand and treat these disorders. ..
  9. Synaptotagmin and C2-Domains: Structure and Function
    JOSE RIZO REY; Fiscal Year: 2010
    ..Moreover, since many neurological disorders are treated with drugs that alter synaptic transmission, this research is expected to provide crucial clues for the development of novel strategies to understand and treat these disorders. ..
  10. Structure and Function of Syntaxin 1
    JOSE RIZO REY; Fiscal Year: 2004
    ....
  11. SYNAPTOTAGMIN AND C2 DOMAINS--STRUCTURE AND FUNCTION
    JOSE RIZO REY; Fiscal Year: 2004
    ..Characterization of these properties will not only yield insights into the functions of C2-domains in neurotransmitter release but will also facilitate future studies that will further clarify these functions. ..
  12. SYNAPTOTAGMIN AND C2 DOMAINS--STRUCTURE AND FUNCTION
    JOSE RIZO REY; Fiscal Year: 2003
    ..Characterization of these properties will not only yield insights into the functions of C2-domains in neurotransmitter release but will also facilitate future studies that will further clarify these functions. ..
  13. STRUCTURE/FUNCTION OF SYNTAXIN 1
    JOSE RIZO REY; Fiscal Year: 1999
    ..Finally, the results will aid in the design of therapies for diseases of the nervous system arising from defects in synaptic transmission. ..
  14. SYNAPTOTAGMIN AND C2 DOMAINS--STRUCTURE AND FUNCTION
    JOSE RIZO REY; Fiscal Year: 2002
    ..Characterization of these properties will not only yield insights into the functions of C2-domains in neurotransmitter release but will also facilitate future studies that will further clarify these functions. ..
  15. STRUCTURE/FUNCTION OF SYNTAXIN 1
    JOSE RIZO REY; Fiscal Year: 2001
    ..Finally, the results will aid in the design of therapies for diseases of the nervous system arising from defects in synaptic transmission. ..
  16. STRUCTURE/FUNCTION OF SYNTAXIN 1
    JOSE RIZO REY; Fiscal Year: 1999
    ..Finally, the results will aid in the design of therapies for diseases of the nervous system arising from defects in synaptic transmission. ..
  17. STRUCTURE/FUNCTION OF SYNTAXIN 1
    JOSE RIZO REY; Fiscal Year: 2000
    ..Finally, the results will aid in the design of therapies for diseases of the nervous system arising from defects in synaptic transmission. ..
  18. Neurotransmitter release machinery: structure and function
    JOSE RIZO REY; Fiscal Year: 2009
    ....
  19. SYNAPTOTAGMIN AND C2 DOMAINS--STRUCTURE AND FUNCTION
    JOSE RIZO REY; Fiscal Year: 2001
    ..Characterization of these properties will not only yield insights into the functions of C2-domains in neurotransmitter release but will also facilitate future studies that will further clarify these functions. ..
  20. Structure and Function of Syntaxin 1
    JOSE RIZO REY; Fiscal Year: 2002
    ....
  21. Structure and Function of Syntaxin 1
    JOSE RIZO REY; Fiscal Year: 2003
    ....
  22. 800 MHZ NMR SPECTROMETER
    JOSE RIZO REY; Fiscal Year: 2002
    ..abstract_text> ..