Research Topics
Species | JOSE RIZO REYSummaryAffiliation: University of Texas Southwestern Medical Center Country: USA Publications
Research Grants
| Collaborators
|
Detail Information
Publications
Phosphatidylinositol phosphates as co-activators of Ca2+ binding to C2 domains of synaptotagmin 1LiYi Li
Department of Neuroscience, Baylor College of Medicine, Houston, Texas 77030, USA
J Biol Chem 281:15845-52. 2006....
Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switchJun Lu
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas, USA
PLoS Biol 4:e192. 2006....
Enlightening molecular mechanisms through study of protein interactionsJosep Rizo
Department of Biophysics, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA
J Mol Cell Biol 4:270-83. 2012..Overall, this research underlines the complexities involved in elucidating molecular mechanisms and how these mechanisms can depend critically on an interplay between strong and weak protein interactions...
The Membrane Fusion Enigma: SNAREs, Sec1/Munc18 Proteins, and Their Accomplices-Guilty as Charged?Josep Rizo
Departments of Biophysics, Biochemistry and Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 email
Annu Rev Cell Dev Biol 28:279-308. 2012..MUN domain activity is likely modulated in diverse presynaptic plasticity processes that depend on Ca(2+) and RIM proteins, among others...- Unraveling the mechanisms of synaptotagmin and SNARE function in neurotransmitter releaseJosep Rizo
Departments of Biochemistry and Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
Trends Cell Biol 16:339-50. 2006..Understanding the functions of these proteins will be crucial to reach a faithful description of the mechanisms of membrane fusion and neurotransmitter release... - Snares and Munc18 in synaptic vesicle fusionJosep Rizo
Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
Nat Rev Neurosci 3:641-53. 2002 - Illuminating membrane fusionJosep Rizo
Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
Proc Natl Acad Sci U S A 103:19611-2. 2006 - Synaptic vesicle fusionJosep Rizo
Department of Biochemistry, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, Texas 75390, USA
Nat Struct Mol Biol 15:665-74. 2008..Elucidation of the release mechanism will require a full understanding of the network of interactions among all these proteins and the membranes... - Are neuronal SNARE proteins Ca2+ sensors?Xiaocheng Chen
Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
J Mol Biol 347:145-58. 2005..These results suggest that the SNAREs do not act directly as Ca2+ receptors but SNARE complex assembly is coupled tightly to Ca2+-sensing during neurotransmitter release... - Three-dimensional structure of the rSly1 N-terminal domain reveals a conformational change induced by binding to syntaxin 5Demet Arac
Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
J Mol Biol 346:589-601. 2005.... - A minimal domain responsible for Munc13 activityJayeeta Basu
Department of Neuroscience, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
Nat Struct Mol Biol 12:1017-8. 2005..Here we identify a large alpha-helical domain of mammalian Munc13-1 that is autonomously folded and is sufficient to rescue the total arrest in neurotransmitter release observed in hippocampal neurons lacking Munc13s... - Crystal structure of the RIM2 C2A-domain at 1.4 A resolutionHan Dai
Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
Biochemistry 44:13533-42. 2005.... - A quaternary SNARE-synaptotagmin-Ca2+-phospholipid complex in neurotransmitter releaseHan Dai
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
J Mol Biol 367:848-63. 2007..We propose a model whereby the highly positive electrostatic potential at the tip of the SSCAP complex helps to induce membrane fusion during release... - Close membrane-membrane proximity induced by Ca(2+)-dependent multivalent binding of synaptotagmin-1 to phospholipidsDemet Arac
Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
Nat Struct Mol Biol 13:209-17. 2006..We propose a model wherein synaptotagmin cooperates with the SNAREs in bringing the synaptic vesicle and plasma membranes together and accelerates membrane fusion through the highly positive electrostatic potential of its C(2)B domain... - Rabphilin regulates SNARE-dependent re-priming of synaptic vesicles for fusionFerenc Deak
Center for Basic Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390 9111, USA
EMBO J 25:2856-66. 2006.... - Conformational switch of syntaxin-1 controls synaptic vesicle fusionStefan H Gerber
Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390 9111, USA
Science 321:1507-10. 2008..Thus, the closed conformation of syntaxin-1 gates the initiation of the synaptic vesicle fusion reaction, which is then mediated by SNARE-complex/Munc18-1 assemblies... - Genetic analysis of synaptotagmin-7 function in synaptic vesicle exocytosisAnton Maximov
Departments of Neuroscience, Molecular Genetics, Pharmacology, and Biochemistry and Howard Hughes Medical Institute, UT Southwestern Medical Center, Dallas, TX 75390 9111, USA
Proc Natl Acad Sci U S A 105:3986-91. 2008.... - Dual modes of Munc18-1/SNARE interactions are coupled by functionally critical binding to syntaxin-1 N terminusMikhail Khvotchev
Department of Neuroscience, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
J Neurosci 27:12147-55. 2007..Viewed together, our data indicate that binding of Munc18 to the syntaxin N terminus unites different modes of Munc18/SNARE interactions and is essential for exocytic membrane fusion... - Distinct domains of complexin I differentially regulate neurotransmitter releaseMingshan Xue
Department of Neuroscience, Baylor College of Medicine, Houston, Texas 77030, USA
Nat Struct Mol Biol 14:949-58. 2007.... - Crystal structure of the RIM1alpha C2B domain at 1.7 A resolutionRong Guan
Department of Biochemistry, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, Texas 75390, USA
Biochemistry 46:8988-98. 2007..Our results provide a structural basis to understand the function of RIM C2B domains and suggest that dimerization may be a crucial aspect of RIM function... - Complexin/synaptotagmin interplay controls acrosomal exocytosisCarlos M Roggero
Laboratorio de Biologia Celular y Molecular, Instituto de Histologia y Embriologia IHEM CONICET, Facultad de Ciencias Medicas, Universidad Nacional de Cuyo, Mendoza 5500, Argentina
J Biol Chem 282:26335-43. 2007..Our results show that the functional interplay between complexin and synaptotagmin has a central role in a physiological secretion event, and that this interplay can be modulated by phosphorylation of the C2B domain... - Structural analysis of conserved oligomeric Golgi complex subunit 2Lorraine F Cavanaugh
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
J Biol Chem 282:23418-26. 2007..These structures may represent platforms for interaction with other trafficking proteins including SNAREs (soluble N-ethylmaleimide factor attachment protein receptors) and Rabs... - A complexin/synaptotagmin 1 switch controls fast synaptic vesicle exocytosisJiong Tang
The Center for Basic Neuroscience, UT Southwestern Medical Center, Dallas, TX 75390, USA
Cell 126:1175-87. 2006.... - Structural basis for the evolutionary inactivation of Ca2+ binding to synaptotagmin 4Han Dai
Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
Nat Struct Mol Biol 11:844-9. 2004.... - The N-terminal domains of syntaxin 7 and vti1b form three-helix bundles that differ in their ability to regulate SNARE complex assemblyWolfram Antonin
Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
J Biol Chem 277:36449-56. 2002..However, they differ in their ability to adopt closed conformations and thus to regulate the assembly of SNARE complexes... - Facile detection of protein-protein interactions by one-dimensional NMR spectroscopyDemet Arac
Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390 9038, USA
Biochemistry 42:2774-80. 2003..A study showing that phospholipids compete with the neuronal core complex for Ca2+-dependent binding to the presynaptic Ca2+-sensor synaptotagmin 1 illustrates the usefulness of the SMRC method in studying multicomponent systems... - Convergence and divergence in the mechanism of SNARE binding by Sec1/Munc18-like proteinsIrina Dulubova
Department of Biochemistry, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas 75390, USA
Proc Natl Acad Sci U S A 100:32-7. 2003.... - Structure/function analysis of Ca2+ binding to the C2A domain of synaptotagmin 1Rafael Fernandez-Chacon
Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, Dallas, Texas 75390 9111, USA
J Neurosci 22:8438-46. 2002..Our data also demonstrate that subtle changes in the biochemical properties of synaptotagmin 1 can result in significant alterations in synaptic responses... - Sly1 binds to Golgi and ER syntaxins via a conserved N-terminal peptide motifTomohiro Yamaguchi
Center for Basic Neuroscience, Department of Molecular Genetics, Howard Hughes Medical Institute, UT Southwestern Medical Center, Dallas, TX 75390, USA
Dev Cell 2:295-305. 2002..These data suggest a potentially general mechanism by which SM proteins could interact with peptides in target proteins independent of core complex assembly and suggest that munc18 binding to syntaxin is an exception... - How Tlg2p/syntaxin 16 'snares' Vps45Irina Dulubova
Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
EMBO J 21:3620-31. 2002..Thus, this mechanism represents the most widespread mode of coupling between syntaxins and SM proteins... - Synaptotagmin function in dense core vesicle exocytosis studied in cracked PC12 cellsOk-Ho Shin
Center for Basic Neuroscience, Department of Molecular Genetics and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 6000 Harry Hines Blvd, Dallas, Texas 75390, USA
Nat Neurosci 5:649-56. 2002.... - Solution structure of the Vam7p PX domainJun Lu
Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
Biochemistry 41:5956-62. 2002.... - NMR measurement of the off rate from the first calcium-binding site of the synaptotagmin I C2A domainOscar Millet
, Universitat de Barcelona, , 08028, Barcelona, Spain
FEBS Lett 516:93-6. 2002..These results are consistent with the proposed role of synaptotagmin I as a calcium sensor in release, but suggest that additional factors may help to accelerate the diffusion of Ca2+ to the sensor... - Ca(2+)-binding mode of the C2A-domain of synaptotagminJosep Rizo
Departments of Biochemistry and Pharmacology, University of Texas Southwestern Medical Centre, Dallas, TX, USA
Methods Mol Biol 172:305-16. 2002 - SNARE function revisitedJosep Rizo
Nat Struct Biol 10:417-9. 2003 - Role of electrostatic and hydrophobic interactions in Ca(2+)-dependent phospholipid binding by the C(2)A-domain from synaptotagmin IStefan H Gerber
Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, Dallas, Texas, USA
Diabetes 51:S12-8. 2002..The complex phospholipid binding mode of synaptotagmins may be important for its role in regulated exocytosis of secretory granules and synaptic vesicles... - Three-dimensional structure of the complexin/SNARE complexXiaocheng Chen
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Neuron 33:397-409. 2002..These results suggest that complexin stabilizes the fully assembled SNARE complex as a key step that enables the exquisitely high speed of Ca(2+)-evoked neurotransmitter release... - A broken alpha -helix in folded alpha -SynucleinSreeganga Chandra
Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9111, USA
J Biol Chem 278:15313-8. 2003..The structural organization of the alpha-helices of alpha-synuclein was not anticipated by sequence analyses and may be important for its pathogenic role... - Evidence for SNARE zippering during Ca2+-triggered exocytosis in PC12 cellsMaria F Matos
Center for Basic Neuroscience, Department of Molecular Genetics and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA
Neuropharmacology 45:777-86. 2003.... - A conformational switch in the Piccolo C2A domain regulated by alternative splicingJesus Garcia
Department of Biochemistry and Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
Nat Struct Mol Biol 11:45-53. 2004..These results reveal a novel mechanism of action of C2 domains and uncover a structural principle that may underlie the alteration of protein function by short alternatively spliced sequences... - Conformation-specific binding of p31(comet) antagonizes the function of Mad2 in the spindle checkpointGuohong Xia
Department of Pharmacology, The University of Texas, Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
EMBO J 23:3133-43. 2004..Therefore, our results suggest that p31(comet) counteracts the function of Mad2 and is required for the silencing of the spindle checkpoint... - Genetic analysis of synaptotagmin 2 in spontaneous and Ca2+-triggered neurotransmitter releaseZhiping P Pang
Department of Molecular Genetics, Center for Basic Neuroscience, Howard Hughes Medical Institute, UT Southwestern Medical Center, Dallas, TX 75390 9111, USA
EMBO J 25:2039-50. 2006.... - Unexpected Ca2+-binding properties of synaptotagmin 9Ok Ho Shin
Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, Dallas, TX 75390 9111, USA
Proc Natl Acad Sci U S A 101:2554-9. 2004..Nevertheless, the C2A domain of Syt 9 functions as a Ca2+-binding module, suggesting that Syts 1 and 9 are Ca2+ sensors with similar Ca2+-binding sequences but distinct properties that indicate nonoverlapping functions... - Insights into mad2 regulation in the spindle checkpoint revealed by the crystal structure of the symmetric mad2 dimerMaojun Yang
Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
PLoS Biol 6:e50. 2008..Collectively, our results establish the existence of a symmetric Mad2 dimer and provide insights into Mad1-assisted conformational activation of Mad2 in the spindle checkpoint... - A Munc13/RIM/Rab3 tripartite complex: from priming to plasticity?Irina Dulubova
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390 8816, USA
EMBO J 24:2839-50. 2005..The modular architecture of alpha-RIMs, with nested binding sites for Rab3 and other targets, may be a general feature of Rab effectors that share homology with the alpha-RIM N-terminal sequence... - Binding of the Munc13-1 MUN domain to membrane-anchored SNARE complexesRong Guan
Department of Biochemistry, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, Texas 75390, USA
Biochemistry 47:1474-81. 2008.... - How much can SNAREs flex their muscles?Josep Rizo
Nat Struct Mol Biol 14:880-2. 2007 - A protein sequence that can encode native structure by disfavoring alternate conformationsW Christian Wigley
Department of Physiology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390-9040, USA
Nat Struct Biol 9:381-8. 2002.... - Intramolecular occlusion of the diacylglycerol-binding site in the C1 domain of munc13-1Nan Shen
Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
Biochemistry 44:1089-96. 2005.... - Solution structure of the RIM1alpha PDZ domain in complex with an ELKS1b C-terminal peptideJun Lu
Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390, USA
J Mol Biol 352:455-66. 2005..This groove is formed, in part, by a set of side-chains that is conserved selectively in RIM PDZ domains and that hence determines, at least in part, their unique specificity... - SNARE-mediated lipid mixing depends on the physical state of the vesiclesXiaocheng Chen
Department of Biochemistry and Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
Biophys J 90:2062-74. 2006.... - Endocytosis of synaptotagmin 1 is mediated by a novel, tryptophan-containing motifNadine Jarousse
University of California San Francisco, Genentech Hall, 600 16th St, San Francisco, California 94143 2140, USA
Traffic 4:468-78. 2003..We conclude that endocytosis of synaptotagmin 1 requires a novel type of internalization signal that is subject to regulation by the rest of the C2B domain... - The Mad2 spindle checkpoint protein has two distinct natively folded statesXuelian Luo
Department of Pharmacology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
Nat Struct Mol Biol 11:338-45. 2004..Our results suggest that the unusual two-state behavior of Mad2 is critical for spindle checkpoint signaling... - The Mad2 spindle checkpoint protein undergoes similar major conformational changes upon binding to either Mad1 or Cdc20Xuelian Luo
Department of Biochemistry, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
Mol Cell 9:59-71. 2002..Our data suggest that, upon checkpoint activation, Mad1 recruits Mad2 to unattached kinetochores and may promote binding of Mad2 to Cdc20... - Three-dimensional structure of an independently folded extracellular domain of human amyloid-beta precursor proteinIrina Dulubova
Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA Irina Dulubova UTSouthwestern edu
Biochemistry 43:9583-8. 2004..The identification of this domain and the availability of its atomic structure will facilitate analysis of APP function and of the role of the extracellular region in the regulation of APP cleavage... - Munc18-1 binds directly to the neuronal SNARE complexIrina Dulubova
Department of Biochemistry and Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA
Proc Natl Acad Sci U S A 104:2697-702. 2007.... - p31comet blocks Mad2 activation through structural mimicryMaojun Yang
Department of Pharmacology, The University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390, USA
Cell 131:744-55. 2007..p31(comet) adopts a fold strikingly similar to that of Mad2 and binds at the dimerization interface of Mad2. Thus, p31(comet) exploits the two-state behavior of Mad2 to block its activation by acting as an "anti-Mad2."..
Research Grants
- Neurotransmitter release machinery: structure and functionJOSE RIZO REY; Fiscal Year: 2010....
- Structure and Function of Syntaxin 1JOSE RIZO REY; Fiscal Year: 2006....
- Synaptotagmin and C2-Domains: Structure and FunctionJOSE RIZO REY; Fiscal Year: 2007....
- Neurotransmitter release machinery: structure and functionJOSE RIZO REY; Fiscal Year: 2007....
- Neurotransmitter release machinery: structure and functionJOSE RIZO REY; Fiscal Year: 2009....
- Synaptotagmin and C2-Domains: Structure and FunctionJOSE RIZO REY; Fiscal Year: 2009..Moreover, since many neurological disorders are treated with drugs that alter synaptic transmission, this research is expected to provide crucial clues for the development of novel strategies to understand and treat these disorders. ..
- Synaptotagmin and C2-Domains: Structure and FunctionJOSE RIZO REY; Fiscal Year: 2010..Moreover, since many neurological disorders are treated with drugs that alter synaptic transmission, this research is expected to provide crucial clues for the development of novel strategies to understand and treat these disorders. ..
- SYNAPTOTAGMIN AND C2 DOMAINS--STRUCTURE AND FUNCTIONJOSE RIZO REY; Fiscal Year: 2004..Characterization of these properties will not only yield insights into the functions of C2-domains in neurotransmitter release but will also facilitate future studies that will further clarify these functions. ..
- STRUCTURE/FUNCTION OF SYNTAXIN 1JOSE RIZO REY; Fiscal Year: 2001..Finally, the results will aid in the design of therapies for diseases of the nervous system arising from defects in synaptic transmission. ..
- 800 MHZ NMR SPECTROMETERJOSE RIZO REY; Fiscal Year: 2002..abstract_text> ..