Camillo Ricordi

Summary

Affiliation: University of Miami
Country: USA

Publications

  1. ncbi request reprint Clinical islet transplantation: advances and immunological challenges
    Camillo Ricordi
    University of Miami School of Medicine, Diabetes Research Institute, Miami, Florida 33136, USA
    Nat Rev Immunol 4:259-68. 2004
  2. doi request reprint Regeneration of pancreatic beta-cell mass for the treatment of diabetes
    Juan Dominguez-Bendala
    University of Miami Leonard M Miller School of Medicine, Diabetes Research Institute, Miami, FL, USA
    Expert Opin Biol Ther 12:731-41. 2012
  3. doi request reprint Impact of pancreatic cold preservation on rat islet recovery and function
    Antonello Pileggi
    Cell Transplant Center, Diabetes Research Institute, University of Miami Leonard M Miller School of Medicine, Miami, FL 33136, USA
    Transplantation 87:1442-50. 2009
  4. pmc Prolactin supplementation to culture medium improves beta-cell survival
    Toshiyuki Yamamoto
    Cell Transplant Center, Diabetes Research Institute, University of Miami Leonard M Miller School of Medicine, Miami, FL, USA
    Transplantation 89:1328-35. 2010
  5. pmc Islet transplantation with alemtuzumab induction and calcineurin-free maintenance immunosuppression results in improved short- and long-term outcomes
    Tatiana Froud
    Clinical Islet Transplant Program, Diabetes Research Institute, Miller School of Medicine, University of Miami, Miami, FL, USA
    Transplantation 86:1695-701. 2008
  6. doi request reprint Long-term metabolic and hormonal effects of exenatide on islet transplant recipients with allograft dysfunction
    Raquel N Faradji
    Clinical Islet Transplant Program, Diabetes Research Institute, University of Miami Leonard M Miller School of Medicine, Miami, FL 33136, USA
    Cell Transplant 18:1247-59. 2009
  7. pmc The use of exenatide in islet transplant recipients with chronic allograft dysfunction: safety, efficacy, and metabolic effects
    Tatiana Froud
    Diabetes Research Institute, University of Miami Leonard M Miller School of Medicine, Miami, FL, USA
    Transplantation 86:36-45. 2008
  8. ncbi request reprint Islet transplantation in type 1 diabetes mellitus using cultured islets and steroid-free immunosuppression: Miami experience
    Tatiana Froud
    Diabetes Research Institute and Departments of Surgery and Radiology, University of Miami, Miami, FL, USA
    Am J Transplant 5:2037-46. 2005
  9. pmc Long-term insulin independence and improvement in insulin secretion after supplemental islet infusion under exenatide and etanercept
    Raquel N Faradji
    Clinical Islet Transplant Program, Diabetes Research Institute, University of Miami Leonard M Miller School of Medicine, Miami, FL, USA
    Transplantation 86:1658-65. 2008
  10. ncbi request reprint Antigen presentation and immune regulatory capacity of immature and mature-enriched antigen presenting (dendritic) cells derived from human bone marrow
    Yide Jin
    Department of Surgery, University of Miami School of Medicine, Miami, FL 33101, USA
    Hum Immunol 65:93-103. 2004

Detail Information

Publications110 found, 100 shown here

  1. ncbi request reprint Clinical islet transplantation: advances and immunological challenges
    Camillo Ricordi
    University of Miami School of Medicine, Diabetes Research Institute, Miami, Florida 33136, USA
    Nat Rev Immunol 4:259-68. 2004
  2. doi request reprint Regeneration of pancreatic beta-cell mass for the treatment of diabetes
    Juan Dominguez-Bendala
    University of Miami Leonard M Miller School of Medicine, Diabetes Research Institute, Miami, FL, USA
    Expert Opin Biol Ther 12:731-41. 2012
    ....
  3. doi request reprint Impact of pancreatic cold preservation on rat islet recovery and function
    Antonello Pileggi
    Cell Transplant Center, Diabetes Research Institute, University of Miami Leonard M Miller School of Medicine, Miami, FL 33136, USA
    Transplantation 87:1442-50. 2009
    ..Islet transplantation success depends on the number and quality of islets transplanted. This study aimed at exploring the molecular mechanisms associated with cold pancreas preservation and their impact on islet cell survival and function...
  4. pmc Prolactin supplementation to culture medium improves beta-cell survival
    Toshiyuki Yamamoto
    Cell Transplant Center, Diabetes Research Institute, University of Miami Leonard M Miller School of Medicine, Miami, FL, USA
    Transplantation 89:1328-35. 2010
    ..We examined the effect of human recombinant PRL (rhPRL) supplementation to the culture media to determine its potential use in the context of clinical islet transplantation...
  5. pmc Islet transplantation with alemtuzumab induction and calcineurin-free maintenance immunosuppression results in improved short- and long-term outcomes
    Tatiana Froud
    Clinical Islet Transplant Program, Diabetes Research Institute, Miller School of Medicine, University of Miami, Miami, FL, USA
    Transplantation 86:1695-701. 2008
    ..Only a minority of islet transplant recipients maintain insulin independence at 5 years under the Edmonton protocol of immunosuppression. New immunosuppressive strategies are required to improve long-term outcomes...
  6. doi request reprint Long-term metabolic and hormonal effects of exenatide on islet transplant recipients with allograft dysfunction
    Raquel N Faradji
    Clinical Islet Transplant Program, Diabetes Research Institute, University of Miami Leonard M Miller School of Medicine, Miami, FL 33136, USA
    Cell Transplant 18:1247-59. 2009
    ..Changes in amylin and proinsulin secretion may reflect more efficient insulin processing. Different degrees of responsiveness to exenatide were identified. These may help guide the clinical management of ITx recipients...
  7. pmc The use of exenatide in islet transplant recipients with chronic allograft dysfunction: safety, efficacy, and metabolic effects
    Tatiana Froud
    Diabetes Research Institute, University of Miami Leonard M Miller School of Medicine, Miami, FL, USA
    Transplantation 86:36-45. 2008
    ..The glucagon-like peptide-1 receptor agonist, exenatide (Byetta, Amylin Pharmaceuticals, CA) has properties that could improve existing islet function, prevent further loss of islet mass and possibly even stimulate islet regeneration...
  8. ncbi request reprint Islet transplantation in type 1 diabetes mellitus using cultured islets and steroid-free immunosuppression: Miami experience
    Tatiana Froud
    Diabetes Research Institute and Departments of Surgery and Radiology, University of Miami, Miami, FL, USA
    Am J Transplant 5:2037-46. 2005
    ....
  9. pmc Long-term insulin independence and improvement in insulin secretion after supplemental islet infusion under exenatide and etanercept
    Raquel N Faradji
    Clinical Islet Transplant Program, Diabetes Research Institute, University of Miami Leonard M Miller School of Medicine, Miami, FL, USA
    Transplantation 86:1658-65. 2008
    ..To improve the engraftment and long-term graft function of SI, exenatide (EXN) and etanercept treatment at islet infusion, and long-term EXN treatment were tested in a non-randomized pilot clinical trial...
  10. ncbi request reprint Antigen presentation and immune regulatory capacity of immature and mature-enriched antigen presenting (dendritic) cells derived from human bone marrow
    Yide Jin
    Department of Surgery, University of Miami School of Medicine, Miami, FL 33101, USA
    Hum Immunol 65:93-103. 2004
    ..The observed in vitro deficiency of this function by immature bone marrow cells may therefore contribute to the immune downregulatory capacity seen in the BMC compartment...
  11. pmc Influence of in vitro and in vivo oxygen modulation on β cell differentiation from human embryonic stem cells
    Sirlene Cechin
    Diabetes Research Institute, Department of Surgery, Department of Microbiology and Immunology, Department of Biomedical Engineering, Department of Medicine, and Department of Cell Biology and Anatomy, Miller School of Medicine, University of Miami, Miami, Florida, USA
    Stem Cells Transl Med 3:277-89. 2014
    ..Our studies confirm the importance of oxygen modulation as a key variable to consider in the design of β-cell differentiation protocols and open the door to future strategies for the transplantation of fully mature β cells. ..
  12. ncbi request reprint Long-term islet allograft survival in nonobese diabetic mice treated with tacrolimus, rapamycin, and anti-interleukin-2 antibody
    R Damaris Molano
    Cell Transplant Center, Diabetes Research Institute, Miami, FL 33136, USA
    Transplantation 75:1812-9. 2003
    ....
  13. ncbi request reprint MicroRNA miR-7 is preferentially expressed in endocrine cells of the developing and adult human pancreas
    Mayrin Correa-Medina
    Cell Transplant Center, Diabetes Research Institute University of Miami Leonard M Miller School of Medicine, 1450 NW 10th Avenue, Miami, FL 33136, USA
    Gene Expr Patterns 9:193-9. 2009
    ....
  14. ncbi request reprint Campath-1H does not alter bone marrow cell regulatory function
    Yide Jin
    Lillian Jean Kaplan Renal Transplant Center of the Division of Transplantation of the Department of Surgery, at the University of Miami, Leonard M Miller School of Medicine, Miami, FL 33101, USA
    Hum Immunol 66:637-43. 2005
    ..These in vitro studies suggest that C1H does not abrogate BMC immunoregulation and thus may allow its lympho-depleting effect to be synergistic...
  15. ncbi request reprint Delivery of TAT/PTD-fused proteins/peptides to islets via pancreatic duct
    Dagmar Klein
    Diabetes Research Institute, University of Miami School of Medicine, 1450 NW 10th Avenue, Miami, FL 33136, USA
    Cell Transplant 14:241-8. 2005
    ..These findings suggest that the transduction of chimeric TAT/PTD proteins can represent an efficient tool of molecular delivery independent of the size, to enhance or modify a specific phenotype at the nuclei or cytoplasmic level...
  16. pmc Early metabolic markers of islet allograft dysfunction
    David A Baidal
    Diabetes Research Institute, Miller School of Medicine, University of Miami, Miami, FL 33136, USA
    Transplantation 87:689-97. 2009
    ..Identification of predictor(s) of islet allograft dysfunction (IGD) might allow for early intervention(s) to preserve functional islet mass...
  17. ncbi request reprint Rescue purification maximizes the use of human islet preparations for transplantation
    Hirohito Ichii
    Diabetes Research Institute, University of Miami School of Medicine, Miami, Florida, USA
    Am J Transplant 5:21-30. 2005
    ..Taken together, our data suggest that RGP islets are comparable in terms of viability and potency to CGP islets. RGP may be of assistance in maximizing the number of islet preparations successfully used in transplant protocols...
  18. ncbi request reprint Riboflavin inhibits IL-6 expression and p38 activation in islet cells
    Lorenzo Cobianchi
    Cell Transplant Center, Diabetes Research Institute, Leonard M Miller School of Medicine, University of Miami, Miami, FL 33136, USA
    Cell Transplant 17:559-66. 2008
    ..This observation, together with the safety profile of riboflavin in the clinical setting, makes it an appealing agent for islet cytoprotection in islet transplantation protocols...
  19. pmc A randomized pilot study of donor stem cell infusion in living-related kidney transplant recipients receiving alemtuzumab
    Gaetano Ciancio
    1 The Lillian Jean Kaplan Renal Transplant Center of the Division of Transplantation, Department of Surgery, University of Miami Leonard M Miller School of Medicine, Miami, FL 2 Division of Nephrology and Hypertension, Department of Medicine, University of Miami Leonard M Miller School of Medicine, Miami, FL 3 Diabetes Research Institute, University of Miami Leonard M Miller School of Medicine, Miami, FL 4 Address correspondence to Dr Gaetano Ciancio, 1801 NW 9th Avenue, Miami, FL 33136
    Transplantation 96:800-6. 2013
    ..Transplant tolerance would remove the need for maintenance immunosuppression while improving survival and quality of life...
  20. pmc Anti-proinflammatory effects of sirolimus on human islet preparations
    Atsuyoshi Mita
    Cell Transplant Center, Diabetes Research Institute, Miami, FL, USA
    Transplantation 86:46-53. 2008
    ..In this study, we assessed the effects of sirolimus supplementation in culture media on human islet preparations, focusing on the anti-proinflammatory aspects...
  21. ncbi request reprint Assessment of cytotoxic lymphocyte gene expression in the peripheral blood of human islet allograft recipients: elevation precedes clinical evidence of rejection
    Dongmei Han
    Diabetes Research Institute, University of Miami School of Medicine, 1450 NW 10th Avenue R 134, Miami, FL 33136, USA
    Diabetes 53:2281-90. 2004
    ..The results suggest that, when taken into consideration with other clinical parameters, elevated CL gene levels may enable prediction of islet allograft loss...
  22. ncbi request reprint Beneficial effects of ischemic preconditioning on pancreas cold preservation
    Anthony R Hogan
    Diabetes Research Institute, University of Miami, Miami, FL 33136, USA
    Cell Transplant 21:1349-60. 2012
    ..The use of IPC of the pancreas may contribute to increasing the quality of donor pancreas for transplantation and to improving organ utilization...
  23. doi request reprint Nutritional status and behavior in subjects with type 1 diabetes, before and after islet transplantation
    Raffaella Poggioli
    Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA
    Transplantation 85:501-6. 2008
    ..To investigate whether changes of nutritional status and behavior are associated with islet transplantation (ITx) and to assess their possible mechanisms...
  24. ncbi request reprint FoxP3 mRNA transcripts and regulatory cells in renal transplant recipients 10 years after donor marrow infusion
    Robert E Cirocco
    Department of Surgery, The Lillian Jean Kaplan Renal Transplant Center of the Division of Transplantation, University of Miami Leonard M Miller School of Medicine, Miami, FL 33101, USA
    Transplantation 83:1611-9. 2007
    ..We questioned if this was associated with putatively regulatory FoxP3 mRNA and cell phenotypes (CD4+CD25+high percentages and high DC2:DC1 ratios) in DBMC-i vs. noninfused controls...
  25. ncbi request reprint The effect of simultaneous CD154 and LFA-1 blockade on the survival of allogeneic islet grafts in nonobese diabetic mice
    Thierry Berney
    Cell Transplant Center, Diabetes Research Institute, University of Miami School of Medicine, 1450 NW 10th Avenue, Miami, FL 33136, USA
    Transplantation 76:1669-74. 2003
    ..In this study, the authors have explored the effect of simultaneous blockade of CD40/CD154 and intercellular adhesion molecule (ICAM)/lymphocyte function-associated antigen (LFA)-1 interactions...
  26. pmc Characterization of pancreatic ductal cells in human islet preparations
    Hirohito Ichii
    Cell Transplant Center, Diabetes Research Institute, University of Miami Leonard M Miller School of Medicine, Miami, FL 33136, USA
    Lab Invest 88:1167-77. 2008
    ....
  27. pmc Nephrin is expressed on the surface of insulin vesicles and facilitates glucose-stimulated insulin release
    Alessia Fornoni
    Diabetes Research Institute, University of Miami L Miller School of Medicine, Miami, Florida, USA
    Diabetes 59:190-9. 2010
    ..The aim of this study was to investigate whether diabetes modulates nephrin expression in human pancreatic islets and to explore the role of nephrin in beta-cell function...
  28. ncbi request reprint Immunosuppression and procedure-related complications in 26 patients with type 1 diabetes mellitus receiving allogeneic islet cell transplantation
    Muhammad M Hafiz
    Diabetes Research Institute, Miller School of Medicine, University of Miami, Miami, FL 33136, USA
    Transplantation 80:1718-28. 2005
    ..The long-term safety and tolerability of this steroid-free immunosuppressive protocol for allogeneic islet transplantation has yet to be determined...
  29. ncbi request reprint Toward maximizing the success rates of human islet isolation: influence of donor and isolation factors
    Gaston M Ponte
    Cell Transplant Center and Clinical Islet Transplant Center, Diabetes Research Institute, University of Miami Leonard M Miller School of Medicine, Miami, FL 33136, USA
    Cell Transplant 16:595-607. 2007
    ..Our data suggest that a sequential, integrated approach is highly desirable to improve the success rate of islet cell processing...
  30. doi request reprint Macroporous three-dimensional PDMS scaffolds for extrahepatic islet transplantation
    EILEEN PEDRAZA
    Diabetes Research Institute, University of Miami, Miami, FL 33136, USA
    Cell Transplant 22:1123-35. 2013
    ..Collectively, these results are promising in that they indicate the potential for transplanting islets into a clinically relevant, extrahepatic site that provides spatial distribution of islets as well as intradevice vascularization...
  31. doi request reprint Remote processing of pancreas can restore normal glucose homeostasis in autologous islet transplantation after traumatic whipple pancreatectomy: technical considerations
    Aisha Khan
    Diabetes Research Institute and Cell Transplant Center, University of Miami, Miami, FL, USA
    Cell Transplant 21:1261-7. 2012
    ....
  32. ncbi request reprint Prolonged allogeneic islet graft survival by protoporphyrins
    Antonello Pileggi
    Cell Transplant Center, Diabetes Research Institute, University of Miami School of Medicine, 1450 NW 10th Avenue R 134, Miami, FL 33136, USA
    Cell Transplant 14:85-96. 2005
    ..All these mechanisms may have contributed to the induction of donor-specific hyporesponsiveness in a proportion of the protoporphyrin-treated animals...
  33. pmc Improved long-term health-related quality of life after islet transplantation
    Thipaporn Tharavanij
    Diabetes Research Institute, University of Miami, Miami, FL 33136, USA
    Transplantation 86:1161-7. 2008
    ..This study aimed to evaluate long-term effects of islet transplantation and to outline possible influential factors...
  34. doi request reprint Nonalbumin proteinuria in islet transplant recipients
    Cristiane B Leitao
    Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA
    Cell Transplant 19:119-25. 2010
    ..Prospective evaluation of proteinuria, including tubular function markers, should be performed to elucidate the mechanisms of kidney damage in this population...
  35. ncbi request reprint A physiological pattern of oxygenation using perfluorocarbon-based culture devices maximizes pancreatic islet viability and enhances β-cell function
    Chris A Fraker
    Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL, USA
    Cell Transplant 22:1723-33. 2013
    ....
  36. doi request reprint Rapamycin impairs in vivo proliferation of islet beta-cells
    Elsie Zahr
    Diabetes Research Institute, University of Miami Leonard Miller School of Medicine, Miami, FL 33136, USA
    Transplantation 84:1576-83. 2007
    ..Progressive graft dysfunction is commonly observed in recipients of islet allografts treated with high doses of rapamycin. This study aimed at evaluating the effect of rapamycin on pancreatic islet cell proliferation in vivo...
  37. doi request reprint Improved metabolic control and quality of life in seven patients with type 1 diabetes following islet after kidney transplantation
    Pablo Cure
    Clinical Islet Transplant Program and Cell Transplant Center, Diabetes Research Institute, Leonard M Miller School of Medicine, University of Miami, Miami, FL 33136, USA
    Transplantation 85:801-12. 2008
    ..The beneficial effects of glycemic control on both survival and function of transplanted kidneys in patients with type 1 diabetes mellitus (T1DM) and end-stage renal disease (ESRD) have been recognized...
  38. ncbi request reprint Protection of human pancreatic islets using a lentiviral vector expressing two genes: cFLIP and GFP
    Elizabeth S Fenjves
    Diabetes Research Institute, University of Miami School of Medicine, Miami, FL, USA
    Cell Transplant 17:793-802. 2008
    ..Furthermore, the engineered islets are resistant to a variety of apoptotic stimuli, suggesting the potential of this approach in enhancing the viability of transplanted cells...
  39. pmc Type 2 diabetes mellitus phenotype and graft survival after islet transplantation
    Cristiane B Leitao
    Diabetes Research Institute, Miami, FL, USA
    Transplantation 88:57-61. 2009
    ..The aim of this study was to evaluate whether insulin resistance, characterized by risk factors for type 2 DM, can predict islet graft survival in type 1 DM islet transplant (ITx) recipients...
  40. ncbi request reprint Systemic overexpression of interleukin-10 fails to protect allogeneic islet transplants in nonobese diabetic mice
    Y Clare Zhang
    Department of Pediatrics, Children s Research Institute, University of South Florida, St Petersburg, FL, USA
    Transplantation 80:530-3. 2005
    ..Thus, IL-10 appears insufficient in protecting transplanted islet cells from allogeneic rejection and suggests important mechanistic variances between alloreactivity and autoimmunity in terms islet graft loss...
  41. pmc Stable renal function after islet transplantation: importance of patient selection and aggressive clinical management
    Cristiane B Leitao
    Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA
    Transplantation 87:681-8. 2009
    ..The aim of this study was to determine clinical, laboratory, and immunosuppressant-related factors associated with kidney dysfunction in islet transplant recipients...
  42. ncbi request reprint Resolution of severe atopic dermatitis after tacrolimus withdrawal
    Gaston M Ponte
    Diabetes Research Institute, University of Miami Leonard M Miller School of Medicine, Miami, FL 33136, USA
    Cell Transplant 16:23-30. 2007
    ..Islet graft function remained stable after modification of the therapeutic regimen (stable glycemic control and unchanged C-peptide)...
  43. ncbi request reprint Resolution of neurotoxicity and beta-cell toxicity in an islet transplant recipient following substitution of tacrolimus with MMF
    Tatiana Froud
    Diabetes Research Institute, University of Miami School of Medicine, Miami, FL 33136, USA
    Cell Transplant 15:613-20. 2006
    ..At 18 months post-"switch," 30 months posttransplant, the patient remains insulin independent with good glycemic control. The goal to remove calcineurin inhibitors from regimens of islet transplantation is a worthy one...
  44. ncbi request reprint Immune responses and their regulation by donor bone marrow cells in clinical organ transplantation
    James M Mathew
    Department of Surgery, Division of Transplantation R 440, University of Miami School of Medicine, 1600 N W 10th Ave, Miami, FL 33136, USA
    Transpl Immunol 11:307-21. 2003
    ..These studies all suggested a strong inhibitory property of a number of DBMC sub-populations in vitro and in vivo with the notion that they promote unresponsiveness...
  45. doi request reprint Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells
    Kamalaveni R Prabakar
    Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA
    Cell Transplant 21:1321-39. 2012
    ..These data suggest that human cord blood may be a promising source for regenerative medicine approaches for the treatment of diabetes mellitus...
  46. ncbi request reprint Dendritic cells in human thymus and periphery display a proinsulin epitope in a transcription-dependent, capture-independent fashion
    Carlos A Garcia
    Immunogenetics Program and Cell Transplant Center, Diabetes Research Institute, Miller School of Medicine, University of Miami, FL 33136, USA
    J Immunol 175:2111-22. 2005
    ..These cells might play a role in promoting self-tolerance, not only in the thymus but also in the periphery...
  47. doi request reprint Clinical use of fructosamine in islet transplantation
    Thipaporn Tharavanij
    Diabetes Research Institute, University of Miami, Miami, FL, USA Department of Medicine, Thammasat University, Pratumthani, Thailand
    Cell Transplant 18:453-8. 2009
    ..Fructosamine can be used as an alternative to HbA1c for glycemic assessment in islet transplant recipients in situations with HbA1c assay interference. Neither HbA1c nor fructosamine are good predictors of islet graft dysfunction...
  48. ncbi request reprint Delivery of Bcl-XL or its BH4 domain by protein transduction inhibits apoptosis in human islets
    Dagmar Klein
    Diabetes Research Institute, University of Miami School of Medicine, Miami, FL, USA
    Biochem Biophys Res Commun 323:473-8. 2004
    ..We conclude that both TAT proteins are biologically active after transduction and could be an asset in the improvement of islet viability...
  49. pmc Lipotoxicity and decreased islet graft survival
    Cristiane B Leitao
    Diabetes Research Institute, Miami, Florida, USA
    Diabetes Care 33:658-60. 2010
    ..Prospective clinical trials should address whether it is directly caused by lipotoxicity and if strategies focusing on lowering serum lipids may prolong islet graft survival...
  50. ncbi request reprint Adenoviral gene transfer of erythropoietin confers cytoprotection to isolated pancreatic islets
    Elizabeth S Fenjves
    Diabetes Research Institute, University of Miami School of Medicine, Miami, FL 33136, USA Efenjves miami edu
    Transplantation 77:13-8. 2004
    ..Because EPO is a cytokine that promotes survival, we examined whether adenovirus-mediated gene transfer of EPO would result in cytoprotection of human pancreatic islets in culture and in the transplant setting...
  51. ncbi request reprint A novel method for the assessment of cellular composition and beta-cell viability in human islet preparations
    Hirohito Ichii
    Diabetes Research Institute, University of Miami, Leonard M Miller School of Medicine, Miami, FL, USA
    Am J Transplant 5:1635-45. 2005
    ....
  52. ncbi request reprint Interference with tissue factor prolongs intrahepatic islet allograft survival in a nonhuman primate marginal mass model
    Dora M Berman
    Diabetes Research Institute, Miller School of Medicine, University of Miami, Miami, FL 33136, USA
    Transplantation 84:308-15. 2007
    ..We tested whether peritransplant protection of islets from IBMIR with a monoclonal anti-TF antibody (CNTO859) would enhance engraftment in our nonhuman primate marginal mass model...
  53. ncbi request reprint Quantitative differential expression analysis reveals miR-7 as major islet microRNA
    Valia Bravo-Egana
    Diabetes Research Institute, University of Miami Leonard M Miller School of Medicine, 1450 NW 10th Avenue, Miami, FL 33136, USA
    Biochem Biophys Res Commun 366:922-6. 2008
    ..Therefore, we conclude that miR-7 is the most abundant endocrine miRNA in islets while miR-375 is the most abundant intra-islet miRNA. Our results may offer new insights into regulatory pathways of islet gene expression...
  54. ncbi request reprint Induction of auto-reactive regulatory T cells by stimulation with immature autologous dendritic cells
    Yide Jin
    The Lillian Jean Kaplan Renal Transplant Center of the Division of Transplantation of the Department of Surgery, University of Miami, Leonard M Miller School of Medicine, Miami, Florida 33101, USA
    Immunol Invest 36:213-32. 2007
    ..This regulation appeared to require cell-to-cell contact...
  55. ncbi request reprint Prolonged islet allograft survival in diabetic NOD mice by targeting CD45RB and CD154
    R Damaris Molano
    Cell Transplant Center, Diabetes Research Institute, University of Miami School of Medicine, Miami, Florida, USA
    Diabetes 52:957-64. 2003
    ..These data show that nondiabetogenic regimens of immunomodulation can lead to prolonged islet allograft survival in the challenging NOD mouse model...
  56. ncbi request reprint TAT-mediated neurogenin 3 protein transduction stimulates pancreatic endocrine differentiation in vitro
    Juan Dominguez-Bendala
    Diabetes Research Institute, University of Miami School of Medicine, 1450 NW 10th Ave, Miami, FL 33136, USA
    Diabetes 54:720-6. 2005
    ..Our results raise the possibility of sequentially specifying stem/progenitor cells toward the beta-cell lineage, by using the appropriate sequence and combination of TAT-fused transcription factors...
  57. ncbi request reprint Regulation of alloimmune responses (GvH reactions) in vitro by autologous donor bone marrow cell preparation used in clinical organ transplantation
    James M Mathew
    Department of Surgery, University of Miami School of Medicine, Florida 33136, USA
    Transplantation 74:846-55. 2002
    ..Therefore, we questioned whether an intrinsic immunoregulatory function of (subpopulations of) DBMC might play a role in this observation...
  58. doi request reprint Antisense miR-7 impairs insulin expression in developing pancreas and in cultured pancreatic buds
    Margarita Nieto
    Diabetes Research Institute, University of Miami, Miller School of Medicine, Miami, FL 33136, USA
    Cell Transplant 21:1761-74. 2012
    ..These findings suggest that modulation of miR-7 expression could be utilized in the development of stem cell therapies to cure diabetes...
  59. pmc Quantitative in situ analysis of FoxP3+ T regulatory cells on transplant tissue using laser scanning cytometry
    Hidenori Takahashi
    Miami Transplant Institute, University of Miami Leonard M Miller School of Medicine, Miami, FL, USA
    Cell Transplant 21:113-25. 2012
    ..This method was also applicable for liver, small intestine, kidney, pancreas, and heart transplant biopsy sections and provide an objective quantification of Tregs within the grafts...
  60. pmc Noninvasive in vivo model demonstrating the effects of autonomic innervation on pancreatic islet function
    Rayner Rodriguez-Diaz
    Diabetes Research Institute, Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, University of Miami Miller School of Medicine, Miami, FL 33136, USA
    Proc Natl Acad Sci U S A 109:21456-61. 2012
    ..This study directly demonstrates that autonomic axons innervating the islet modulate glucose homeostasis...
  61. pmc Restoration of hypoglycemia awareness after islet transplantation
    Cristiane B Leitao
    Diabetes Research Institute, Miami, Florida, USA
    Diabetes Care 31:2113-5. 2008
    ..To determine the impact of islet transplantation (ITx) on hypoglycemia awareness in patients with unstable type 1 diabetes and its relation to islet function...
  62. ncbi request reprint Protecting pancreatic beta-cells
    Antonello Pileggi
    Diabetes Research Institute, University of Miami School of Medicine, Miami, Florida 33136, USA
    IUBMB Life 56:387-94. 2004
    ..In this review we present description of chemical and molecular biology based strategies to confer cytoprotection to beta-cells...
  63. pmc Automated, high-throughput assays for evaluation of human pancreatic islet function
    Over Cabrera
    Diabetes Research Institute, Miller School of Medicine, University of Miami, Miami, FL 33136, USA
    Cell Transplant 16:1039-48. 2008
    ..These methodologies will enable functional characterization of human islet preparations before transplantation and thereby provide the basis for the establishment of predictive tests for beta-cell potency...
  64. ncbi request reprint The use of the BD oxygen biosensor system to assess isolated human islets of langerhans: oxygen consumption as a potential measure of islet potency
    Chris Fraker
    Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA
    Cell Transplant 15:745-58. 2006
    ..27 took significantly longer, often failing to reverse at all over a 35-day time period. These results suggest that the OBS could be a useful tool for the pretransplant assessment of islet cell potency...
  65. ncbi request reprint Human donor bone marrow cells induce in vitro "suppressor T cells" that functionally suppress autologous B cells
    Manuel R Carreno
    Departments of Surgery, Division of Transplantation, University of Miami School of Medicine, Miami, FL 33101, USA
    Hum Immunol 64:21-30. 2003
    ..These in vitro findings support the notion that (allogeneic) DBMC infusions can induce a T4 suppressor (regulatory) influence and thereby indirectly affect B-cell function...
  66. ncbi request reprint CD40-CD40 ligand interaction activates proinflammatory pathways in pancreatic islets
    Florencia M Barbé-Tuana
    Diabetes Research Institute, University of Miami Leonard M Miller School of Medicine, Miami, FL 33136, USA
    Diabetes 55:2437-45. 2006
    ..Our results in vitro indicate that the CD40 receptor expressed by beta-cells could be activated in vivo, inducing proinflammatory responses contributing to early islet graft loss after transplantation...
  67. ncbi request reprint A novel micro-cell-mediated lympholytic assay for the evaluation of regulatory cells in human alloreactive CTL responses
    James M Mathew
    Division of Transplantation, Department of Surgery, University of Miami School of Medicine, R 440, 1600 N W 10th Ave, FL 33136, USA
    J Immunol Methods 272:67-80. 2003
    ....
  68. pmc ATP-gated P2X3 receptors constitute a positive autocrine signal for insulin release in the human pancreatic beta cell
    M Caroline Jacques-Silva
    Diabetes Research Institute, Miller School of Medicine, University of Miami, Miami, FL 33136, USA
    Proc Natl Acad Sci U S A 107:6465-70. 2010
    ..This may explain how the human pancreatic beta cell can respond so effectively to relatively modest changes in glucose concentration under physiological conditions in vivo...
  69. ncbi request reprint The l-isoform but not d-isoforms of a JNK inhibitory peptide protects pancreatic beta-cells
    Alessia Fornoni
    Diabetes Research Institute, University of Miami Miller School of Medicine, 1450 NW 10th Avenue, Miami, FL 33136, USA
    Biochem Biophys Res Commun 354:227-33. 2007
    ..In conclusion, L-JNKI directly affects beta-cells and ameliorates islet viability and function, while RI-JNKI has toxic effects, limiting its biological application to islet cell biology...
  70. ncbi request reprint Retrovirally transferred genes inhibit apoptosis in an insulin-secreting cell line: implications for islet transplantation
    Elizabeth S Fenjves
    Diabetes Research Institute, University of Miami, School of Medicine, Miami, FL 33136, USA
    Cell Transplant 13:489-96. 2004
    ..These genes may be ideal candidates to augment islet survival following transplantation...
  71. ncbi request reprint Targeted bone marrow radioablation with 153Samarium-lexidronam promotes allogeneic hematopoietic chimerism and donor-specific immunologic hyporesponsiveness
    Luca Inverardi
    Diabetes Research Institute, University of Miami School of Medicine, Miami, Florida, USA
    Transplantation 77:647-55. 2004
    ..We studied whether targeted delivery of radiation to bone marrow could allow for bone marrow cell (BMC) engraftment, chimerism, and donor-specific tolerance in the absence of the side effects associated with external irradiation...
  72. pmc MicroRNA signature of the human developing pancreas
    Samuel Rosero
    Diabetes Research Institute, University of Miami, Miller School of Medicine, Miami, FL 33136, USA
    BMC Genomics 11:509. 2010
    ....
  73. ncbi request reprint Nonlethal conditioning for the induction of allogeneic chimerism and tolerance to islet allografts
    Hua Li
    Cell Transplant Center, Diabetes Research Institute, University of Miami School of Medicine, 1450 NW 10th Avenue R 134, Miami, FL 33136, USA
    Transplantation 75:966-70. 2003
    ..In the present study, we evaluated the outcome of islet allografts implanted either simultaneously or after donor bone marrow cell (BMC) infusion when nonlethal conditioning was used...
  74. ncbi request reprint The use of multiparametric monitoring during islet cell isolation and culture: a potential tool for in-process corrections of critical physiological factors
    Chris Fraker
    Diabetes Research Institute, Cell Transplant Center, University of Miami School of Medicine, Miami, FL 33136, USA
    Cell Transplant 13:497-502. 2004
    ....
  75. ncbi request reprint Peripheral blood cytotoxic lymphocyte gene transcript levels differ in patients with long-term type 1 diabetes compared to normal controls
    Dongmei Han
    Diabetes Research Institute, University of Miami School of Medicine, 1450 N W 10th Avenue, Miami, FL 33136, USA
    Cell Transplant 14:403-9. 2005
    ..05). The decreased expression of P and FasL in patients with long-term type 1 diabetes might contribute to the inability to maintain normal levels of peripheral tolerance, which is essential for protection from autoimmune disease...
  76. ncbi request reprint Heme oxygenase-1 fused to a TAT peptide transduces and protects pancreatic beta-cells
    Melina M Ribeiro
    Diabetes Research Institute, University of Miami School of Medicine, 1450 NW 10th Avenue R 134, Miami, FL 33136, USA
    Biochem Biophys Res Commun 305:876-81. 2003
    ..Finally, we report that transduction of HO-1 fusion protein into islets improves islet viability in culture. This approach might have a positive impact on the availability of islets for transplantation...
  77. ncbi request reprint Overcoming the challenges now limiting islet transplantation: a sequential, integrated approach
    Antonello Pileggi
    Cell Transplant Center, Diabetes Research Institute, Miller School of Medicine, University of Miami, 1450 NW 10th Avenue R 134, Miami, FL 33136, USA
    Ann N Y Acad Sci 1079:383-98. 2006
    ..In order to overcome the current hurdles to the widespread application of islet transplantation there is a need for implementation of integrated, sequential therapeutic approaches...
  78. ncbi request reprint Deterioration and variability of highly purified collagenase blends used in clinical islet isolation
    Toshiyuki Yamamoto
    Cell Transplant Center, Diabetes Research Institute, University of Miami Leonard M Miller School of Medicine, Miami, FL 33136, USA
    Transplantation 84:997-1002. 2007
    ..This study examines the interlot and intralot variability of Liberase HI and the over-time deterioration of enzyme quality based on the analysis of islet isolation outcomes...
  79. ncbi request reprint Islet xenotransplantation
    Raffaella Poggioli
    Diabetes Research Institute, University of Miami School of Medicine, FL 33136, USA
    Cell Transplant 11:89-94. 2002
  80. ncbi request reprint Suramin inhibits the CD40-CD154 costimulatory interaction: a possible mechanism for immunosuppressive effects
    Emilio Margolles-Clark
    Diabetes Research Institute, Miller School of Medicine, University of Miami, FL, USA
    Biochem Pharmacol 77:1236-45. 2009
    ....
  81. doi request reprint Isolation and expansion of human natural T regulatory cells for cellular therapy
    Rajendra Pahwa
    Diabetes Research Institute, University of Miami, Leonard M Miller School of Medicine, Miami, FL 33136, USA
    J Immunol Methods 363:67-79. 2010
    ....
  82. ncbi request reprint Neonatal porcine pancreatic cell clusters as a potential source for transplantation in humans: characterization of proliferation, apoptosis, xenoantigen expression and gene delivery with recombinant AAV
    Caterina Vizzardelli
    Diabetes Research Institute, Cell Transplant Center, University of Miami School of Medicine, FL 33136, USA
    Xenotransplantation 9:14-24. 2002
    ..This characterization might be helpful in the definition of the potential use of neonatal porcine islets for human transplantation...
  83. ncbi request reprint Allogeneic islet transplantation
    Simona Marzorati
    University of Miami Miller School of Medicine, Cell Transplant Center and Clinical Islet Transplant Program, Diabetes Research Institute, 1450 NW, 10th Avenue R 134, Miami, FL 33136, USA
    Expert Opin Biol Ther 7:1627-45. 2007
    ..The progress in beta-cell replacement treatment by islet transplantation as well as those of emerging immune interventions for the restoration of self tolerance justify great optimism for years to come...
  84. ncbi request reprint Islet transplantation: immunological perspectives
    Luca Inverardi
    Diabetes Research Institute R 134, University of Miami School of Medicine, 1450 NW 10th Avenue, Miami, FL 33136, USA
    Curr Opin Immunol 15:507-11. 2003
    ..If the use of xenografts is successful, it might represent a solution to the shortage of human organs...
  85. ncbi request reprint Functional and phenotypic properties of peripheral T cells anergized by autologous CD3(+) depleted bone marrow cells
    Yide Jin
    Department of Surgery, University of Miami School of Medicine, Miami, FL 33136, USA
    Hum Immunol 63:567-75. 2002
    ..These in vitro observations may provide a mechanism by which infused donor BMC serve to downregulate T-cell immunity...
  86. doi request reprint Minimization and withdrawal of steroids in pancreas and islet transplantation
    Davide Mineo
    Diabetes Research Institute, University of Miami, FL 33136, USA
    Transpl Int 22:20-37. 2009
    ..Main limitations are allo- and autoimmunity, immunosuppression-related islet and systemic toxicity and transplant site unsuitability, which tolerogenic protocols and biotechnological solutions may solve...
  87. ncbi request reprint Delivery of proteins and peptides into live cells by means of protein transduction domains: potential application to organ and cell transplantation
    Ricardo L Pastori
    Diabetes Research Institute, University of Miami School of Medicine, Miami, FL 33136, USA
    Transplantation 77:1627-31. 2004
  88. ncbi request reprint Allosensitization of islet allograft recipients
    Roberta Cardani
    Clinical Islet Transplant Program, Cell Transplant Center, Diabetes Research Institute, Leonard M Miller School of Medicine, University of Miami, Miami, FL, USA
    Transplantation 84:1413-27. 2007
    ..A negative association of PRA+ with allogeneic solid organ graft survival has been recognized, but scattered data is available for islet transplantation...
  89. pmc Recurrence of type 1 diabetes after simultaneous pancreas-kidney transplantation, despite immunosuppression, is associated with autoantibodies and pathogenic autoreactive CD4 T-cells
    Francesco Vendrame
    Diabetes Research Institute, Leonard Miller School of Medicine, University of Miami, Miami, Florida, USA
    Diabetes 59:947-57. 2010
    ..To investigate if recurrent autoimmunity explained hyperglycemia and C-peptide loss in three immunosuppressed simultaneous pancreas-kidney (SPK) transplant recipients...
  90. pmc Current status of islet cell transplantation
    Hirohito Ichii
    Cell Transplant Center, Diabetes Research Institute, University of Miami Leonard M Miller School of Medicine, Miami, FL 33136, USA
    J Hepatobiliary Pancreat Surg 16:101-12. 2009
    ....
  91. ncbi request reprint Use of D-STAT to prevent bleeding following percutaneous transhepatic intraportal islet transplantation
    Tatiana Froud
    University of Miami, Cell Transplant Division of Surgery, Miami, FL 33136, USA
    Cell Transplant 13:55-9. 2004
    ..This new method may simplify tract closure and reduce bleeding complications in islet transplantation...
  92. ncbi request reprint Human, nonhuman primate, and rat pancreatic islets express erythropoietin receptors
    Elizabeth S Fenjves
    Diabetes Research Institute, University of Miami, School of Medicine, Miami, FL 33136, USA
    Transplantation 75:1356-60. 2003
    ..The authors examined pancreatic islets for the presence of EPO-R to determine whether these cells are protected by EPO from cytokine-induced apoptosis...
  93. ncbi request reprint Protein transduction: a novel approach to induce in vitro pancreatic differentiation
    Juan Dominguez-Bendala
    Pancreatic Development and Stem Cell Laboratory, Diabetes Research Institute, University of Miami Leonard M Miller School of Medicine, 1450 NW 10th Ave, Miami, FL 33136, USA
    Cell Transplant 15:S85-90. 2006
    ..Here we discuss this novel application of the flourishing PT technology, which may revolutionize the way we direct stem cells along any specific lineage...
  94. doi request reprint Quantitative assessment of islet cell products: estimating the accuracy of the existing protocol and accounting for islet size distribution
    Peter Buchwald
    Diabetes Research Institute, University of Miami Leonard M Miller School of Medicine, Miami, FL 33136, USA
    Cell Transplant 18:1223-35. 2009
    ..Compared to the current calculation method, these factors introduce a 4-8% downward correction of the total IEQ estimate, but they reflect a statistically more accurate contribution of differently sized islets...
  95. ncbi request reprint Requirements for success in clinical islet transplantation
    Camillo Ricordi
    The Diabetes Research Institute at University of Miami School of Medicine, Miami, Florida 33136, USA
    Transplantation 79:1298-300. 2005
    ....
  96. ncbi request reprint Effect of living-related donor bone marrow infusion on chimerism and in vitro immunoregulatory activity in kidney transplant recipients
    Gaetano Ciancio
    Department of Surgery, Division of Transplantation, University of Miami School of Medicine, Miami, FL 33101, USA E mail
    Transplantation 74:488-96. 2002
    ..4% in the iliac crest bone marrow compartment now at 6 years). Prompted by this, we embarked on a study of DBMC infusion in living-related donor (LRD) kidney transplant recipients...
  97. ncbi request reprint Efficient transduction of pancreatic islets by feline immunodeficiency virus vectors1
    Michael A Curran
    Department of Microbiology and Immunology, Stanford University Medical Center, California, USA
    Transplantation 74:299-306. 2002
    ..Genetic engineering of islets before transplantation with protective genes may enhance their posttransplantation survival. Accomplishing this goal requires the development of a safe, efficient vector for islet gene delivery...
  98. ncbi request reprint Improved human islet isolation outcome from marginal donors following addition of oxygenated perfluorocarbon to the cold-storage solution
    Camillo Ricordi
    Diabetes Research Institute, University of Miami School of Medicine, Florida 33136, USA
    Transplantation 75:1524-7. 2003
    ..To this end, the two-layer method may help clinicians overcome the problem of organ underuse...
  99. ncbi request reprint Adeno-associated virus-mediated IL-10 gene therapy inhibits diabetes recurrence in syngeneic islet cell transplantation of NOD mice
    Y Clare Zhang
    Department of Pathology, University of Florida, 1600 SW Archer Road, Gainesville, FL 32610 0275, USA
    Diabetes 52:708-16. 2003
    ..These studies support the utility of immunoregulatory cytokine gene therapy delivered by rAAV for preventing autoimmune disease recurrence in transplant-based therapies for type 1 diabetes...
  100. ncbi request reprint Compaction of islets is detrimental to transplant outcome in mice
    Shaheed Merani
    Department of Surgery, Faculty of Medicine, University of Alberta, Edmonton AB, Canada
    Transplantation 82:1472-6. 2006
    ..We herein investigate the potential detrimental impact of maintaining islets in a pelleted state for periods preceding implantation. We hypothesize that periods of islet compaction lead to impairment if islet function in vivo...

Research Grants23

  1. Human Pancreatic Islet Cell Resource to Catalyze Collaborative Diabetes Research
    Camillo Ricordi; Fiscal Year: 2006
    ..Continue making human islets of high quality available to ICR-approved centers, for clinical and research applications. ..
  2. ISLET CELL RESOURCE FOR DIABETES RESEARCH AND TREATMENT
    Camillo Ricordi; Fiscal Year: 2006
    ..4. Develop isolation, preservation and shipment procedures that result in maximal islet cell function upon transplantation. ..
  3. Strategies to Improve Long Term Islet Graft Survival
    Camillo Ricordi; Fiscal Year: 2007
    ..abstract_text> ..
  4. Human Pancreatic Islet Cell Resource to Catalyze Collaborative Diabetes Research
    Camillo Ricordi; Fiscal Year: 2007
    ..Continue making human islets of high quality available to ICR-approved centers, for clinical and research applications. ..
  5. PATHWAYS TO TOLERANCE IN HUMAN ISLET TRANSPLANTATION
    Camillo Ricordi; Fiscal Year: 2004
    ..Specific Aim #2: To assess metabolic function of successful intrahepatic islet allografts. Specific Aim #3: To study immune alterations that lead to tolerance in islet allograft recipients. ..
  6. PANCREATIC TRANSPLANTATION AND DIABETES MELLITUS
    Camillo Ricordi; Fiscal Year: 1999
    ..The application of this information should, as well, be generally applicable to the field of organ transplantation. ..
  7. PATHWAYS TO TOLERANCE IN HUMAN ISLET TRANSPLANTATION
    Camillo Ricordi; Fiscal Year: 2000
    ..Specific Aim #2: To assess metabolic function of successful intrahepatic islet allografts. Specific Aim #3: To study immune alterations that lead to tolerance in islet allograft recipients. ..
  8. PATHWAYS TO TOLERANCE IN HUMAN ISLET TRANSPLANTATION
    Camillo Ricordi; Fiscal Year: 2001
    ..Specific Aim #2: To assess metabolic function of successful intrahepatic islet allografts. Specific Aim #3: To study immune alterations that lead to tolerance in islet allograft recipients. ..
  9. ISLET CELL RESOURCE FOR DIABETES RESEARCH AND TREATMENT
    Camillo Ricordi; Fiscal Year: 2001
    ..4. Develop isolation, preservation and shipment procedures that result in maximal islet cell function upon transplantation. ..
  10. PATHWAYS TO TOLERANCE IN HUMAN ISLET TRANSPLANTATION
    Camillo Ricordi; Fiscal Year: 2002
    ..Specific Aim #2: To assess metabolic function of successful intrahepatic islet allografts. Specific Aim #3: To study immune alterations that lead to tolerance in islet allograft recipients. ..
  11. ISLET CELL RESOURCE FOR DIABETES RESEARCH AND TREATMENT
    Camillo Ricordi; Fiscal Year: 2002
    ..4. Develop isolation, preservation and shipment procedures that result in maximal islet cell function upon transplantation. ..
  12. PANCREATIC TRANSPLANTATION AND DIABETIC MELLITUS
    Camillo Ricordi; Fiscal Year: 2002
    ..Achievement of the proposed goals will allow us to extend islet transplantation as a therapeutic option to a large cohort of patients with Type 1 diabetes ..
  13. PATHWAYS TO TOLERANCE IN HUMAN ISLET TRANSPLANTATION
    Camillo Ricordi; Fiscal Year: 2003
    ..Specific Aim #2: To assess metabolic function of successful intrahepatic islet allografts. Specific Aim #3: To study immune alterations that lead to tolerance in islet allograft recipients. ..
  14. ISLET CELL RESOURCE FOR DIABETES RESEARCH AND TREATMENT
    Camillo Ricordi; Fiscal Year: 2003
    ..4. Develop isolation, preservation and shipment procedures that result in maximal islet cell function upon transplantation. ..
  15. Human Pancreatic Islet Cell Resource to Catalyze Collaborative Diabetes Research
    Camillo Ricordi; Fiscal Year: 2009
    ..Continue making human islets of high quality available to ICR-approved centers, for clinical and research applications. ..