Bruce Richardson

Summary

Affiliation: University of Michigan
Country: USA

Publications

  1. pmc Stronger inflammatory/cytotoxic T-cell response in women identified by microarray analysis
    A Hewagama
    Department of Internal Medicine, Rheumatology Division, The University of Michigan, Ann Arbor, MI 48109 2200, USA
    Genes Immun 10:509-16. 2009
  2. doi request reprint Murine models of lupus induced by hypomethylated T cells (DNA hypomethylation and lupus…)
    Bruce Richardson
    University of Michigan and the Ann Arbor Veteran s Affairs Hospital, Ann Arbor, MI, USA
    Methods Mol Biol 900:169-80. 2012
  3. ncbi request reprint Role of DNA methylation in the regulation of cell function: autoimmunity, aging and cancer
    Bruce C Richardson
    Department of Medicine, University of Michigan and the Veterans Affairs Hospital, Ann Arbor 48109 0940, USA
    J Nutr 132:2401S-2405S. 2002
  4. ncbi request reprint Primer: epigenetics of autoimmunity
    Bruce Richardson
    University of Michigan and Ann Arbor Veterans Affairs Hospital, Ann Arbor, MI, USA
    Nat Clin Pract Rheumatol 3:521-7. 2007
  5. ncbi request reprint DNA methylation and autoimmune disease
    Bruce Richardson
    Department of Medicine at the University of Michigan and the Ann Arbor VA Hospital, Ann Arbor, MI 48109 0940, USA
    Clin Immunol 109:72-9. 2003
  6. ncbi request reprint Impact of aging on DNA methylation
    Bruce Richardson
    Department of Medicine, University of Michigan, 5310 Cancer Center and Geriatrics Center Building, Ann Arbor, MI 48109 0940, USA
    Ageing Res Rev 2:245-61. 2003
  7. doi request reprint Epigenetics and autoimmunity. Overview
    Bruce C Richardson
    The Department of Medicine, University of Michigan, 3018 BSRB, 109 Zina Pitcher Pl, Ann Arbor, MI 48109 2200, USA
    Autoimmunity 41:243-4. 2008
  8. ncbi request reprint Overexpression of CD70 and overstimulation of IgG synthesis by lupus T cells and T cells treated with DNA methylation inhibitors
    Kurt Oelke
    University of Michigan, Ann Arbor, 48109, USA
    Arthritis Rheum 50:1850-60. 2004
  9. ncbi request reprint Murine models of lupus induced by hypomethylated T cells
    Bruce Richardson
    Department of Internal Medicine, University of Michigan and the Ann Arbor Veteran s Affairs Hospital, Ann Arbor, MI, USA
    Methods Mol Med 102:285-94. 2004
  10. ncbi request reprint Demethylation of ITGAL (CD11a) regulatory sequences in systemic lupus erythematosus
    Qianjin Lu
    Cancer Center and Geriatric Center, University of Michigan, Ann Arbor, MI 48109, USA
    Arthritis Rheum 46:1282-91. 2002

Research Grants

  1. Environmental effects on lupus T cell DNA methylation and gene expression
    Bruce Richardson; Fiscal Year: 2009
  2. Role of T-cells in the induction of lupus
    Bruce C Richardson; Fiscal Year: 2010
  3. Aberrant gene expression in CD4+CD28-T cells: mechanisms
    Bruce Richardson; Fiscal Year: 2007
  4. Role of T-cells in the induction of lupus
    Bruce Richardson; Fiscal Year: 2007
  5. Environmental effects on lupus T cell DNA methylation and gene expression
    Bruce Richardson; Fiscal Year: 2007
  6. Aberrant gene expression in CD4+CD28-T cells: mechanisms
    Bruce C Richardson; Fiscal Year: 2010
  7. GENDER-SPECIFIC T CELL HOMING AND AUTOIMMUNITY
    Bruce Richardson; Fiscal Year: 2003
  8. SIGNIFICANCE OF AGE DEPENDENT CHANGES IN DNA METHYLATION
    Bruce Richardson; Fiscal Year: 2002
  9. T CELLS AND THE INDUCTION OF LUPUS
    Bruce Richardson; Fiscal Year: 2001

Collaborators

Detail Information

Publications45

  1. pmc Stronger inflammatory/cytotoxic T-cell response in women identified by microarray analysis
    A Hewagama
    Department of Internal Medicine, Rheumatology Division, The University of Michigan, Ann Arbor, MI 48109 2200, USA
    Genes Immun 10:509-16. 2009
    ..The differential expression of inflammatory/cytotoxic effector molecules in restimulated female T cells may contribute to the differences in autoimmune diseases between women and men...
  2. doi request reprint Murine models of lupus induced by hypomethylated T cells (DNA hypomethylation and lupus…)
    Bruce Richardson
    University of Michigan and the Ann Arbor Veteran s Affairs Hospital, Ann Arbor, MI, USA
    Methods Mol Biol 900:169-80. 2012
    ..This chapter describes the protocols for inducing autoreactivity in murine T cells in vitro and for inducing autoimmunity in vivo using an adoptive transfer approach or transgenic animal models...
  3. ncbi request reprint Role of DNA methylation in the regulation of cell function: autoimmunity, aging and cancer
    Bruce C Richardson
    Department of Medicine, University of Michigan and the Veterans Affairs Hospital, Ann Arbor 48109 0940, USA
    J Nutr 132:2401S-2405S. 2002
    ..Other mechanisms are likely to be identified as well...
  4. ncbi request reprint Primer: epigenetics of autoimmunity
    Bruce Richardson
    University of Michigan and Ann Arbor Veterans Affairs Hospital, Ann Arbor, MI, USA
    Nat Clin Pract Rheumatol 3:521-7. 2007
    ..Similar mechanisms might also contribute to the pathogenesis of other poorly understood human diseases...
  5. ncbi request reprint DNA methylation and autoimmune disease
    Bruce Richardson
    Department of Medicine at the University of Michigan and the Ann Arbor VA Hospital, Ann Arbor, MI 48109 0940, USA
    Clin Immunol 109:72-9. 2003
    ..T cells from patients with active lupus have evidence for most if not all of the same methylation abnormalities, suggesting that abnormal DNA methylation plays a role in idiopathic human lupus as well...
  6. ncbi request reprint Impact of aging on DNA methylation
    Bruce Richardson
    Department of Medicine, University of Michigan, 5310 Cancer Center and Geriatrics Center Building, Ann Arbor, MI 48109 0940, USA
    Ageing Res Rev 2:245-61. 2003
    ..As this field is still developing, it is anticipated that new knowledge will continue to accumulate rapidly...
  7. doi request reprint Epigenetics and autoimmunity. Overview
    Bruce C Richardson
    The Department of Medicine, University of Michigan, 3018 BSRB, 109 Zina Pitcher Pl, Ann Arbor, MI 48109 2200, USA
    Autoimmunity 41:243-4. 2008
  8. ncbi request reprint Overexpression of CD70 and overstimulation of IgG synthesis by lupus T cells and T cells treated with DNA methylation inhibitors
    Kurt Oelke
    University of Michigan, Ann Arbor, 48109, USA
    Arthritis Rheum 50:1850-60. 2004
    ..We then determined whether CD70 is overexpressed on T cells treated with other DNA methylation inhibitors and on SLE T cells, and determined its functional significance...
  9. ncbi request reprint Murine models of lupus induced by hypomethylated T cells
    Bruce Richardson
    Department of Internal Medicine, University of Michigan and the Ann Arbor Veteran s Affairs Hospital, Ann Arbor, MI, USA
    Methods Mol Med 102:285-94. 2004
    ..This chapter describes the protocols for inducing autoreactivity in murine T cells in vitro and using the cells to induce autoimmunity in vivo...
  10. ncbi request reprint Demethylation of ITGAL (CD11a) regulatory sequences in systemic lupus erythematosus
    Qianjin Lu
    Cancer Center and Geriatric Center, University of Michigan, Ann Arbor, MI 48109, USA
    Arthritis Rheum 46:1282-91. 2002
    ..The present study investigated the nature of the methylation change that affects LFA-1 expression in vitro and in human lupus...
  11. ncbi request reprint Demethylation of promoter regulatory elements contributes to perforin overexpression in CD4+ lupus T cells
    Mariana J Kaplan
    Department of Medicine, University of Michigan, Ann Arbor, MI 48109, USA
    J Immunol 172:3652-61. 2004
    ..Our results also suggest that aberrant perforin expression in CD4+ lupus T cells may contribute to monocyte killing...
  12. pmc DNA methylation inhibition increases T cell KIR expression through effects on both promoter methylation and transcription factors
    Ying Liu
    Department of Medicine, University of Michigan, USA
    Clin Immunol 130:213-24. 2009
    ..This was confirmed for Sp1. These results indicate that KIR genes are suppressed by DNA methylation in most T cells, and DNA demethylation promotes their expression through effects on both chromatin structure and transcription factors...
  13. ncbi request reprint Impaired T cell protein kinase C delta activation decreases ERK pathway signaling in idiopathic and hydralazine-induced lupus
    Gabriela Gorelik
    Department of Medicine, University of Michigan, Ann Arbor, MI 48109, USA
    J Immunol 179:5553-63. 2007
    ..These results suggest that defective T cell PKCdelta activation may contribute to the development of idiopathic and hydralazine-induced lupus through effects on T cell DNA methylation...
  14. ncbi request reprint Effect of DNA methylation and chromatin structure on ITGAL expression
    Qianjin Lu
    Department of Medicine, University of Michigan and the Ann Arbor VA Hospital, 48109 0940, USA
    Blood 99:4503-8. 2002
    ..The results thus indicate that methylation and chromatin structure may contribute to the tissue-specific expression of CD11a...
  15. pmc Aberrant T cell ERK pathway signaling and chromatin structure in lupus
    Gabriela Gorelik
    Department of Medicine, University of Michigan, Ann Arbor, MI 48109 2200, USA
    Autoimmun Rev 8:196-8. 2009
    ....
  16. pmc Age-dependent decreases in DNA methyltransferase levels and low transmethylation micronutrient levels synergize to promote overexpression of genes implicated in autoimmunity and acute coronary syndromes
    Yepeng Li
    Dept of Medicine, University of Michigan, Ann Arbor, 48109, USA
    Exp Gerontol 45:312-22. 2010
    ..Thus, attention to proper nutrition may be particularly important in the elderly...
  17. pmc Overexpression of X-linked genes in T cells from women with lupus
    Anura Hewagama
    Department of Internal Medicine, Division of Rheumatology, University of Michigan, Ann Arbor, MI 48109 2200, USA
    J Autoimmun 41:60-71. 2013
    ..Together these results further support a role for X chromosome demethylation in the female predisposition to lupus...
  18. ncbi request reprint Aging in heterozygous Dnmt1-deficient mice: effects on survival, the DNA methylation genes, and the development of amyloidosis
    Donna Ray
    Department of Medicine, University of Michigan, Ann Arbor, USA
    J Gerontol A Biol Sci Med Sci 61:115-24. 2006
    ..This model thus provides potential mechanisms for DNA methylation changes of aging, and suggests that changes in DNA methylation may contribute to some forms of amyloidosis that develop with aging...
  19. ncbi request reprint Decreased T cell ERK pathway signaling may contribute to the development of lupus through effects on DNA methylation and gene expression
    Kurt Oelke
    Department of Medicine, University of Michigan, and the Ann Arbor Veterans Affairs Hospital, Ann Arbor, Michigan
    Int Rev Immunol 23:315-31. 2004
    ..This mechanism could contribute to idiopathic and drug-induced lupus...
  20. ncbi request reprint DNA methylation and chromatin structure regulate T cell perforin gene expression
    Qianjin Lu
    University of Michigan, Ann Arbor, MI 48109, USA
    J Immunol 170:5124-32. 2003
    ..These results indicate that DNA methylation and chromatin structure participate in the regulation of perforin expression in T cells...
  21. pmc Key role of ERK pathway signaling in lupus
    Gabriela Gorelik
    Department of Medicine, University of Michigan, Ann Arbor, MI 48109 2200, USA
    Autoimmunity 43:17-22. 2010
    ..Understanding the mechanism causing decreased ERK pathway signaling in lupus may shed light on mechanisms contributing to disease development in genetically predisposed people...
  22. pmc The genetics and epigenetics of autoimmune diseases
    Anura Hewagama
    Department of Medicine, University of Michigan, Ann Arbor, MI 48109 2200, USA
    J Autoimmun 33:3-11. 2009
    ..Other autoimmune diseases may have a similar foundation...
  23. pmc Sex-specific differences in the relationship between genetic susceptibility, T cell DNA demethylation and lupus flare severity
    Amr H Sawalha
    Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    J Autoimmun 38:J216-22. 2012
    ....
  24. pmc Decreased DNA methyltransferase levels contribute to abnormal gene expression in "senescent" CD4(+)CD28(-) T cells
    Ying Liu
    Department of Medicine, University of Michigan, USA
    Clin Immunol 132:257-65. 2009
    ..We conclude that decreased Dnmt1 and Dnmt3a cause demethylation and overexpression of these and perhaps other genes in CD4(+)CD28(-) cells, potentially contributing to pathologic functions by this subset...
  25. ncbi request reprint Infrequent occurrence of age-dependent changes in CpG island methylation as detected by restriction landmark genome scanning
    John Tra
    Department of Pediatrics and Infectious Diseases, University of Michigan, Ann Arbor, MI 48109, USA
    Mech Ageing Dev 123:1487-503. 2002
    ..These results indicate that the methylation status of the majority of CpG islands in both mice and humans is tightly controlled during aging, and that changes are infrequent and in humans confined to a specific subset of genes...
  26. pmc Hypermethylation of PTGER2 confers prostaglandin E2 resistance in fibrotic fibroblasts from humans and mice
    Steven K Huang
    Department of Internal Medicine, University of Michigan Medical School, 6301 MSRB III, 1150 West Medical Center Drive, Ann Arbor, MI 48109, USA
    Am J Pathol 177:2245-55. 2010
    ....
  27. ncbi request reprint The apoptotic ligands TRAIL, TWEAK, and Fas ligand mediate monocyte death induced by autologous lupus T cells
    Mariana J Kaplan
    Department of Internal Medicine, University of Michigan, 1150 West Medical Center Drive, 5220 Medical Science Research Building I, Ann Arbor, MI 48109, USA
    J Immunol 169:6020-9. 2002
    ..We propose that this mechanism could provide a source of potentially antigenic material for the autoimmune response and interfere with normal clearing mechanisms...
  28. pmc Epigenetics in human autoimmunity. Epigenetics in autoimmunity - DNA methylation in systemic lupus erythematosus and beyond
    Faith M Strickland
    The Department of Medicine, University of Michigan, Ann Arbor, MI 48109 2200, USA
    Autoimmunity 41:278-86. 2008
    ..Herein we review how disruption of epigenetic mechanisms can alter immune function using lupus as an example, and summarize how similar mechanisms may contribute to other human autoimmune rheumatic and skin diseases...
  29. ncbi request reprint Tissue-specific effect of estradiol on endothelial cell-dependent lymphocyte recruitment
    Hedwig S Murphy
    Department of Pathology, University of Michigan, Ann Arbor, Michigan 48109, USA
    Microvasc Res 68:273-85. 2004
    ..The gender-specific increase in lymphocyte homing to spleen may be attributable, at least in part, to tissue-specific estrogen-mediated effects on microvascular endothelial cells...
  30. ncbi request reprint DNaseI hypersensitivity analysis of chromatin structure
    Qianjin Lu
    Cancer Center, University of Michigan, Ann Arbor, USA
    Methods Mol Biol 287:77-86. 2004
    ..This chapter describes the protocols necessary to perform and analyze DNaseI hypersensitivity assays, a technique becoming increasingly important given the rapid advances in our understanding of the chromatin remodeling processes...
  31. ncbi request reprint Age-dependent DNA methylation changes in the ITGAL (CD11a) promoter
    Zhiyong Zhang
    Department of Medicine, Veterans Affairs Hospital, 5310 Cancer Center and Geriatrics Center Building, University of Michigan, Ann Arbor, MI 48109 0940, USA
    Mech Ageing Dev 123:1257-68. 2002
    ....
  32. pmc Stimulatory and inhibitory killer Ig-like receptor molecules are expressed and functional on lupus T cells
    Dhiman Basu
    Department of Medicine, University of Michigan, Ann Arbor, MI 48109, USA
    J Immunol 183:3481-7. 2009
    ..These results indicate that aberrant T cell KIR expression may contribute to IFN overproduction and macrophage killing in human lupus, and they suggest that Abs to inhibitory KIR may be a treatment for this disease...
  33. pmc Decreased ERK and JNK signaling contribute to gene overexpression in "senescent" CD4+CD28- T cells through epigenetic mechanisms
    Yingxuan Chen
    Department of Medicine, University of Michigan, Ann Arbor, MI 48109 2200, USA
    J Leukoc Biol 87:137-45. 2010
    ..We conclude that decreased ERK and JNK signaling in the CD4+CD28- subset, arising with replicative stress, can lead to the overexpression of normally suppressed genes through effects on Dnmts and consequently, chromatin structure...
  34. ncbi request reprint Pathogenesis of lupus
    Kurt Oelke
    University of Michigan, and Ann Arbor Veterans Affairs Hospital, Ann Arbor, Michigan USA
    Arthritis Rheum 47:343-5. 2002
  35. ncbi request reprint Relative quantitation of DNA methyltransferase mRNA by real-time RT-PCR assay
    John Attwood
    Cancer Center, University of Michigan, Ann Arbor, USA
    Methods Mol Biol 287:273-83. 2004
    ..This technique is both sensitive and specific and allows for the rapid assessment of Dnmt mRNA transcript levels as well transcripts for other genes that may be involved in DNA methylation...
  36. ncbi request reprint Demethylation of the same promoter sequence increases CD70 expression in lupus T cells and T cells treated with lupus-inducing drugs
    Qianjin Lu
    Department of Medicine, University of Michigan, Ann Arbor, MI 48109, USA
    J Immunol 174:6212-9. 2005
    ....
  37. pmc Epigenetic mechanisms in lupus
    Dipak R Patel
    University of Michigan, Ann Arbor Veterans Affairs Hospital, Ann Arbor, Michigan, USA
    Curr Opin Rheumatol 22:478-82. 2010
    ..We first focus on the roles of DNA demethylation and DNA methyltransferase enzyme dysregulation, and we then review recent and important advances in this field...
  38. ncbi request reprint Endothelial cell apoptosis in systemic lupus erythematosus: a common pathway for abnormal vascular function and thrombosis propensity
    Sanjay Rajagopalan
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109 0680, USA
    Blood 103:3677-83. 2004
    ..Heightened endothelial apoptosis may represent an important mechanism for development of atherothrombosis in SLE...
  39. ncbi request reprint Accelerated macrophage apoptosis induces autoantibody formation and organ damage in systemic lupus erythematosus
    Michael F Denny
    Department of Internal Medicine, University of Michigan, Ann Arbor, 48109, USA
    J Immunol 176:2095-104. 2006
    ..This study suggests that mechanisms that induce scavenger cell apoptosis, such as death induced by autoreactive cytotoxic T cells observed in SLE, could play a pathogenic role and contribute to the severity of the disease...
  40. ncbi request reprint Estrogen regulates CCR gene expression and function in T lymphocytes
    RuRan Mo
    Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, USA
    J Immunol 174:6023-9. 2005
    ..Our results indicate an important role of estrogen in determining T cell chemokine response that may help explain the increased susceptibility and severity of autoimmune diseases in females...
  41. ncbi request reprint Attenuation of autoimmune disease in Fas-deficient mice by treatment with a cytotoxic benzodiazepine
    Jeffrey J Bednarski
    University of Michigan, Ann Arbor, 48109, USA
    Arthritis Rheum 48:757-66. 2003
    ..We investigated the Fas-dependence of its cytotoxic actions, its therapeutic potential in mice deficient in Fas, and its therapeutic mechanism of action...
  42. ncbi request reprint Age-related changes in mature CD4+ T cells: cell cycle analysis
    Timothy J Hale
    Department of Epidemiology, University of Michigan, Ann Arbor, MI 48109 0940, USA
    Cell Immunol 220:51-62. 2002
    ..This was associated with a significant decline in cyclin D2 mRNA and protein. We propose that decreases in cyclin D2 are at least partially responsible for the proliferative decline found in aged CD4(+) T cells...
  43. pmc Benzodiazepine-induced superoxide signals B cell apoptosis: mechanistic insight and potential therapeutic utility
    Neal B Blatt
    Department of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, Michigan 48109 1055, USA
    J Clin Invest 110:1123-32. 2002
    ....
  44. ncbi request reprint Abnormal T cell signal transduction in systemic lupus erythematosus
    Gary M Kammer
    Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
    Arthritis Rheum 46:1139-54. 2002
  45. ncbi request reprint The epigenetic face of systemic lupus erythematosus
    Esteban Ballestar
    Cancer Epigenetics Laboratory, Molecular Pathology Programme, Spanish National Cancer Centre CNIO, Madrid, Spain
    J Immunol 176:7143-7. 2006
    ..In the present study, we discuss the implications of epigenetic alterations in the development and progression of SLE and how epigenetic drugs constitute a promising source of therapy to treat this disease...

Research Grants36

  1. Environmental effects on lupus T cell DNA methylation and gene expression
    Bruce Richardson; Fiscal Year: 2009
    ..Evidence that dietary modification can ameliorate aberrant gene expression in vitro and disease severity in the murine model will lead to studies extending these results to lupus patients. ..
  2. Role of T-cells in the induction of lupus
    Bruce C Richardson; Fiscal Year: 2010
    ..The studies described in this application will figure out how and why it is damaged, and confirm that the damage causes autoimmunity. Understanding how this happens will suggest ways to fix the problem. ..
  3. Aberrant gene expression in CD4+CD28-T cells: mechanisms
    Bruce Richardson; Fiscal Year: 2007
    ..These studies will identify mechanisms modifying gene expression in this pathologic subset, and suggest ways to restore normal gene expression in these cells. ..
  4. Role of T-cells in the induction of lupus
    Bruce Richardson; Fiscal Year: 2007
    ..We anticipate that these studies will provide new insights into mechanisms causing lupus-like illnesses, and identify new approaches to the treatment of this sometimes fatal disease. ..
  5. Environmental effects on lupus T cell DNA methylation and gene expression
    Bruce Richardson; Fiscal Year: 2007
    ..Evidence that dietary modification can ameliorate aberrant gene expression in vitro and disease severity in the murine model will lead to studies extending these results to lupus patients. ..
  6. Aberrant gene expression in CD4+CD28-T cells: mechanisms
    Bruce C Richardson; Fiscal Year: 2010
    ..These studies will identify mechanisms modifying gene expression in this pathologic subset, and suggest ways to restore normal gene expression in these cells. ..
  7. GENDER-SPECIFIC T CELL HOMING AND AUTOIMMUNITY
    Bruce Richardson; Fiscal Year: 2003
    ..Dr. Richardson anticipates that these studies will identify novel and important mechanisms contributing to the increased incidence and severity of autoimmune disease in women. ..
  8. SIGNIFICANCE OF AGE DEPENDENT CHANGES IN DNA METHYLATION
    Bruce Richardson; Fiscal Year: 2002
    ..This approach may also prove useful in characterizing genes that have altered expression with aging in lymphocytes and other cell types, and that may contribute to the development of autoimmunity and malignancies in the elderly. ..
  9. T CELLS AND THE INDUCTION OF LUPUS
    Bruce Richardson; Fiscal Year: 2001
    ..The principal investigator anticipates that these studies will clarify mechanisms contributing to the development of human lupus. ..