Markus Walter Ribbe


Affiliation: University of California
Country: USA


  1. Sickerman N, Hu Y, Ribbe M. Nitrogenases. Methods Mol Biol. 2019;1876:3-24 pubmed publisher
    ..This chapter discusses the structure and function of nitrogenase, covers methods that have proven useful in the elucidation of enzyme properties, and provides an overview of the three known nitrogenase variants. ..
  2. Lee C, Ribbe M, Hu Y. Purification of Nitrogenase Proteins. Methods Mol Biol. 2019;1876:111-124 pubmed publisher
    ..In addition, purification and Fe-S reconstitution strategies are also outlined for the heterologously expressed nitrogenase proteins in Escherichia coli. ..
  3. Jasniewski A, Hu Y, Ribbe M. Electron Paramagnetic Resonance Spectroscopy of Metalloproteins. Methods Mol Biol. 2019;1876:197-211 pubmed publisher
    ..Here, a basic introduction to the theory of EPR and the instrumentation is presented along with procedures for obtaining EPR spectra of sensitive metalloprotein species. ..
  4. Rettberg L, Tanifuji K, Jasniewski A, Ribbe M, Hu Y. Radical S-Adenosyl-l-Methionine (SAM) Enzyme Involved in the Maturation of the Nitrogenase Cluster. Methods Enzymol. 2018;606:341-361 pubmed publisher
    ..This chapter details the procedures necessary to prepare an active NifB protein. The methods used for the biochemical characterization of the SAM-dependent carbide insertion reactions are also described. ..
  5. Lee C, Hu Y, Ribbe M. Insights into hydrocarbon formation by nitrogenase cofactor homologs. MBio. 2015;6: pubmed publisher
    ..The reported assay system provides a useful tool for mechanistic investigations of this reaction while suggesting the possibility of designing bioinspired catalysts based on nitrogenase cofactors. ..
  6. Ribbe M. Insights into the Mechanism of Carbon Monoxide Dehydrogenase at Atomic Resolution. Angew Chem Int Ed Engl. 2015;54:8337-9 pubmed publisher
  7. Tanifuji K, Lee C, Ohki Y, Tatsumi K, Hu Y, Ribbe M. Combining a Nitrogenase Scaffold and a Synthetic Compound into an Artificial Enzyme. Angew Chem Int Ed Engl. 2015;54:14022-5 pubmed publisher
    ..The successful outcome of this work provides the proof of concept and underlying principles for continued search of novel enzymatic activities based on this approach. ..
  8. Rebelein J, Hu Y, Ribbe M. Widening the Product Profile of Carbon Dioxide Reduction by Vanadium Nitrogenase. Chembiochem. 2015;16:1993-6 pubmed publisher
    ..These observations afford initial insights into the characteristics of this unique reaction and provide a potential template for future design of catalysts to recycle the greenhouse gas CO2 into useful products. ..
  9. Tanifuji K, Lee C, Sickerman N, Tatsumi K, Ohki Y, Hu Y, et al. Tracing the 'ninth sulfur' of the nitrogenase cofactor via a semi-synthetic approach. Nat Chem. 2018;10:568-572 pubmed publisher
    ..This work provides a semi-synthetic tool for protein reconstitution that could be widely applicable for the functional analysis of other FeS systems. ..


More Information


  1. Lee C, Sickerman N, Hu Y, Ribbe M. YedY: A Mononuclear Molybdenum Enzyme with a Redox-Active Ligand?. Chembiochem. 2016;17:453-5 pubmed publisher
    ..A recent electrochemical investigation suggests that the mononuclear molybdenum enzyme YdeY utilizes redox-active ligands during catalysis. ..
  2. Hu Y, Ribbe M. Nitrogenases-A Tale of Carbon Atom(s). Angew Chem Int Ed Engl. 2016;55:8216-26 pubmed publisher
  3. Hu Y, Ribbe M. Maturation of nitrogenase cofactor-the role of a class E radical SAM methyltransferase NifB. Curr Opin Chem Biol. 2016;31:188-94 pubmed publisher
  4. Sickerman N, Hu Y, Ribbe M. Nitrogenase Assembly: Strategies and Procedures. Methods Enzymol. 2017;595:261-302 pubmed publisher
    ..Furthermore, protocols for nitrogenase protein purification and substrate reduction activity assays are described. These methods provide a solid framework for the assessment of nitrogenase assembly and catalysis. ..