W S Reznikoff

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. pmc Defining characteristics of Tn5 Transposase non-specific DNA binding
    Mindy Steiniger
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Nucleic Acids Res 34:2820-32. 2006
  2. pmc Comparative sequence analysis of IS50/Tn5 transposase
    William S Reznikoff
    Department of Biochemistry, University of Wisconsin, 433 Babcock Dr, Madison, WI 53706, USA
    J Bacteriol 186:8240-7. 2004
  3. ncbi request reprint Tn5 transposition: a molecular tool for studying protein structure-function
    W S Reznikoff
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Biochem Soc Trans 34:320-3. 2006
  4. ncbi request reprint Tn5: A molecular window on transposition
    W S Reznikoff
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin, 53706, USA
    Biochem Biophys Res Commun 266:729-34. 1999
  5. ncbi request reprint Tn5 as a model for understanding DNA transposition
    William S Reznikoff
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Mol Microbiol 47:1199-206. 2003
  6. ncbi request reprint Three-dimensional structure of the Tn5 synaptic complex transposition intermediate
    D R Davies
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Science 289:77-85. 2000
  7. ncbi request reprint The three-dimensional structure of a Tn5 transposase-related protein determined to 2.9-A resolution
    D R Davies
    Institute for Enzyme Research and Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53705, USA
    J Biol Chem 274:11904-13. 1999
  8. ncbi request reprint Functional characterization of arginine 30, lysine 40, and arginine 62 in Tn5 transposase
    S S Twining
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53705, USA
    J Biol Chem 276:23135-43. 2001
  9. ncbi request reprint Tn5 transposase loops DNA in the absence of Tn5 transposon end sequences
    Christian D Adams
    Department of Biochemistry, University of Wisconsin at Madison, 433 Babcock Drive, WI 53706, USA
    Mol Microbiol 62:1558-68. 2006
  10. ncbi request reprint Dissecting Tn5 transposition using HIV-1 integrase diketoacid inhibitors
    Agata Czyz
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin 53706, USA
    Biochemistry 46:10776-89. 2007

Collaborators

  • S S Twining
  • John F Marko
  • R J Noel
  • Mindy Steiniger-White
  • Dunja Skoko
  • Brandon Ason
  • Todd A Naumann
  • Christian D Adams
  • Igor Y Goryshin
  • Richard J Gradman
  • Kelly M Winterberg
  • Mindy Steiniger
  • Agata Czyz
  • D R Davies
  • Vadim A Klenchin
  • Soheila Vaezeslami
  • Bernhard Schnurr
  • I Rayment
  • Jerod L Ptacin
  • Archna Bhasin
  • Richard Gradman
  • Scott Lovell
  • H Yigit
  • Ivan Rayment
  • Kara A Stillmock
  • Daria J Hazuda
  • Rachel Sterling
  • Jeremy Metzler
  • Daniel J Knauss
  • Allison M Balke
  • Anna Marie Skalka
  • George Merkel
  • Amanda S Bruegl
  • John Luecke
  • D Czarniecki
  • Jennifer Apodaca
  • I Y Goryshin
  • L Mahnke Braam
  • R A Jilk
  • L Borchardt
  • J C Makris

Detail Information

Publications33

  1. pmc Defining characteristics of Tn5 Transposase non-specific DNA binding
    Mindy Steiniger
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Nucleic Acids Res 34:2820-32. 2006
    ....
  2. pmc Comparative sequence analysis of IS50/Tn5 transposase
    William S Reznikoff
    Department of Biochemistry, University of Wisconsin, 433 Babcock Dr, Madison, WI 53706, USA
    J Bacteriol 186:8240-7. 2004
    ..The results were also used to compare the IS50 transposase with the more distantly related transposase encoded by IS10...
  3. ncbi request reprint Tn5 transposition: a molecular tool for studying protein structure-function
    W S Reznikoff
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Biochem Soc Trans 34:320-3. 2006
    ..The success of these forward genetic technologies requires that the transposition system be efficient and manifest near-random target sequence selection...
  4. ncbi request reprint Tn5: A molecular window on transposition
    W S Reznikoff
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin, 53706, USA
    Biochem Biophys Res Commun 266:729-34. 1999
    ..However, most of the insights should be of general interest because of similarities to other transposition-like systems such as HIV-1 DNA integration into the host genome...
  5. ncbi request reprint Tn5 as a model for understanding DNA transposition
    William S Reznikoff
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Mol Microbiol 47:1199-206. 2003
    ..Transposase is a very inactive protein. An inactive transposase protein ensures the survival of the host and thus the survival of Tn5...
  6. ncbi request reprint Three-dimensional structure of the Tn5 synaptic complex transposition intermediate
    D R Davies
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Science 289:77-85. 2000
    ....
  7. ncbi request reprint The three-dimensional structure of a Tn5 transposase-related protein determined to 2.9-A resolution
    D R Davies
    Institute for Enzyme Research and Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53705, USA
    J Biol Chem 274:11904-13. 1999
    ..A novel C-terminal domain forms a dimer interface across a crystallographic 2-fold axis. Although this dimer represents the structure of the inhibited complex, it provides insight into the structure of the synaptic complex...
  8. ncbi request reprint Functional characterization of arginine 30, lysine 40, and arginine 62 in Tn5 transposase
    S S Twining
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53705, USA
    J Biol Chem 276:23135-43. 2001
    ..These results suggest that lysine 40 is important for a step in the transposition mechanism that is bypassed in the in vitro assay system, such as the removal of the transposase molecule from DNA following strand transfer...
  9. ncbi request reprint Tn5 transposase loops DNA in the absence of Tn5 transposon end sequences
    Christian D Adams
    Department of Biochemistry, University of Wisconsin at Madison, 433 Babcock Drive, WI 53706, USA
    Mol Microbiol 62:1558-68. 2006
    ..Assembly of end sequence-independent nucleoprotein complexes likely plays a role in the in vivo downregulation of transposition and the cis-transposition bias of many bacterial transposases...
  10. ncbi request reprint Dissecting Tn5 transposition using HIV-1 integrase diketoacid inhibitors
    Agata Czyz
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin 53706, USA
    Biochemistry 46:10776-89. 2007
    ..Thus, DKA inhibitors will provide an important set of tools to investigate the mechanism of action of transposases and integrases...
  11. pmc Escherichia coli DNA topoisomerase I copurifies with Tn5 transposase, and Tn5 transposase inhibits topoisomerase I
    H Yigit
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Bacteriol 181:3185-92. 1999
    ..For RNA polymerase, reduced copurification with Tnp is observed in extracts from a topA mutant strain, suggesting that RNA polymerase interacts with Topo I and not Tnp...
  12. pmc Implications of Tn5-associated adjacent deletions
    R A Jilk
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison 53706
    J Bacteriol 175:1264-71. 1993
    ....
  13. ncbi request reprint Pulling apart catalytically active Tn5 synaptic complexes using magnetic tweezers
    Christian D Adams
    Department of Biochemistry, University of Wisconsin at Madison, WI 53706, USA
    J Mol Biol 367:319-27. 2007
    ..These results have implications for the in vivo down regulation of transposition and the cis-transposition bias of transposase...
  14. ncbi request reprint Structure/function insights into Tn5 transposition
    Mindy Steiniger-White
    University of Wisconsin Madison, Department of Biochemistry, 433 Babcock Drive, Madison, WI 53706, USA
    Curr Opin Struct Biol 14:50-7. 2004
    ..Protein-protein contacts required for synapsis are also seen. Finally, the two divalent metals in the active site of the transposase support a 'two-metal-ion' mechanism for Tn5 transposition...
  15. ncbi request reprint Evidence for "unseen" transposase--DNA contacts
    Mindy Steiniger-White
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    J Mol Biol 322:971-82. 2002
    ..A recently determined co-crystal structure of Tn5 Tnp with the ME is discussed and a model explaining possible roles for the base-pair at position 4 is explored...
  16. pmc A high-throughput assay for Tn5 Tnp-induced DNA cleavage
    Brandon Ason
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    Nucleic Acids Res 32:e83. 2004
    ..Furthermore, since Tn5 Tnp shares a similar active site architecture to other transposase/integrase superfamily members, this strategy and any effectors identified using this method will be readily applicable to these other systems...
  17. pmc The -45 region of the Escherichia coli lac promoter: CAP-dependent and CAP-independent transcription
    D Czarniecki
    Department of Biochemistry, University of Wisconsin Madison, 53706, USA
    J Bacteriol 179:423-9. 1997
    ..The mutant promoters also displayed other characteristics, such as the activation of nascent promoter-like activities overlapping lac P1 and, in one case, replicon-dependent changes in promoter activity...
  18. ncbi request reprint Tn5 transposase active site mutants
    Todd A Naumann
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 277:17623-9. 2002
    ..Mutations at Lys-333 reduce the ability of Tnps to form protein-DNA complexes, whereas mutations at the less strongly conserved Lys-330 have less of an effect on both synaptic complex formation and catalytic activity...
  19. pmc Phosphate coordination and movement of DNA in the Tn5 synaptic complex: role of the (R)YREK motif
    Vadim A Klenchin
    Department of Biochemistry, University of Wisconsin at Madison, 433 Babcock Drive, Madison, WI 53706, USA
    Nucleic Acids Res 36:5855-62. 2008
    ..It is likely that this role for the newly identified RYR triad is utilized by other transposase-related proteins...
  20. pmc Tn5 synaptic complex formation: role of transposase residue W450
    Richard J Gradman
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Bacteriol 190:1484-7. 2008
    ..This study demonstrates that W450 is involved in hydrophobic (and possibly aromatic) contacts within the Tnp monomer that negatively regulate synaptic complex formation...
  21. ncbi request reprint Tn5 as a molecular genetics tool: In vitro transposition and the coupling of in vitro technologies with in vivo transposition
    William S Reznikoff
    Department of Biochemistry, University of Wisconsin, Madison, USA
    Methods Mol Biol 260:83-96. 2004
    ..We will also describe a unique in vitro/in vivo technology in which Tn5 inserts can be generated in a wide spectrum of bacterial species through the electroporation of preformed tranposase-transposon DNA complexes...
  22. pmc Mutation of Tn5 transposase beta-loop residues affects all steps of Tn5 transposition: the role of conformational changes in Tn5 transposition
    Mindy Steiniger
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53562, USA
    Biochemistry 45:15552-62. 2006
    ....
  23. pmc Tn5 transposase with an altered specificity for transposon ends
    Todd A Naumann
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Bacteriol 184:233-40. 2002
    ..This protein contains a critical glutamate-to-valine mutation at amino acid 58 that is responsible for this change in end specificity...
  24. pmc A bifunctional DNA binding region in Tn5 transposase
    Richard J Gradman
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Mol Microbiol 67:528-40. 2008
    ....
  25. ncbi request reprint DNA sequence bias during Tn5 transposition
    Brandon Ason
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1544, USA
    J Mol Biol 335:1213-25. 2004
    ..In addition, the fact that several donor DNA sequences, which favor synapsis, appear to serve as hot spots for transposon insertion suggest that similar criteria may exist for Tnp-donor DNA and Tnp-target DNA interactions...
  26. pmc Targeting Tn5 transposase identifies human immunodeficiency virus type 1 inhibitors
    Brandon Ason
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1544, USA
    Antimicrob Agents Chemother 49:2035-43. 2005
    ....
  27. pmc Phenotypic screening of Escherichia coli K-12 Tn5 insertion libraries, using whole-genome oligonucleotide microarrays
    Kelly M Winterberg
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Dr, Madison, WI 53706 1544, USA
    Appl Environ Microbiol 71:451-9. 2005
    ..coli chromosome were identified. Tn5 insertions were detected in 15 genes for which no previous insertions have been reported. Other applications of this method are discussed...
  28. pmc Production of combinatorial libraries of fused genes by sequential transposition reactions
    Todd A Naumann
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
    Nucleic Acids Res 30:e119. 2002
    ..Comparative structural analysis suggests that both sides of the linker are inserted into disordered regions in functional proteins...
  29. pmc Chromosomal deletion formation system based on Tn5 double transposition: use for making minimal genomes and essential gene analysis
    Igor Y Goryshin
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    Genome Res 13:644-53. 2003
    ..This methodology was used to analyze 15 chromosomal regions and more than 100 open reading frames (ORFs). This provides a robust technology for identifying essential and dispensable genes...
  30. ncbi request reprint Mutational analysis of the base flipping event found in Tn5 transposition
    Brandon Ason
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 277:11284-91. 2002
    ..In addition, our results suggested that T2-Tnp interactions facilitate hairpin formation and hairpin resolution primarily through base stacking and that T2 plays a role in the alignment of the transposon DNA for strand transfer...
  31. pmc Site-directed mutagenesis studies of tn5 transposase residues involved in synaptic complex formation
    Soheila Vaezeslami
    Bay Paul Center, Marine Biological Laboratory, Woods Hole, MA 02543, USA
    J Bacteriol 189:7436-41. 2007
    ..By using genetic and biochemical assays, we showed that in all cases except one, each of these residues plays an important role in synaptic complex formation, as predicted by the cocrystal structure...
  32. ncbi request reprint Screening transposon mutant libraries using full-genome oligonucleotide microarrays
    Kelly M Winterberg
    Department of Biology, University of Utah, Salt Lake City, UT, USA
    Methods Enzymol 421:110-25. 2007
    ..Details for the in vivo Tn5 mutagenesis procedure, mutant library construction and competitive outgrowth, T7 in vitro transcription/labeling, and microarray data analysis will be provided...
  33. doi request reprint Transposon-based strategies for the identification of essential bacterial genes
    William S Reznikoff
    Marine Biological Laboratory, Bay Paul Center for Comparative Molecular Biology and Evolution, Woods Hole, MA, USA
    Methods Mol Biol 416:13-26. 2008
    ..This latter approach has the potential to experimentally define the minimal required genome for a given organism...