OSVALDO A REY

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Negative cross-talk between calcium-sensing receptor and β-catenin signaling systems in colonic epithelium
    Osvaldo Rey
    Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, Department of Medicine, CURE Digestive Diseases Research Center, David Geffen School of Medicine, UCLA, Los Angeles, California 90095, USA
    J Biol Chem 287:1158-67. 2012
  2. pmc Extracellular calcium sensing receptor stimulation in human colonic epithelial cells induces intracellular calcium oscillations and proliferation inhibition
    Osvaldo Rey
    Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, Department of Medicine, CURE, Digestive Diseases Research Center, Molecular Biology Institute and Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, University of California at Los Angeles, California 90095 1786, USA
    J Cell Physiol 225:73-83. 2010
  3. ncbi Requirement of the TRPC1 cation channel in the generation of transient Ca2+ oscillations by the calcium-sensing receptor
    Osvaldo Rey
    Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, 900 Veteran Avenue, Los Angeles, CA 90095, USA
    J Biol Chem 281:38730-7. 2006
  4. ncbi The nuclear import of protein kinase D3 requires its catalytic activity
    Osvaldo Rey
    Division of Digestive Diseases, Department of Medicine, CURE Digestive Diseases Research Center and Molecular Biology Institute, David Geffen School of Medicine, University of California, Los Angeles, California 90095 1786, USA
    J Biol Chem 281:5149-57. 2006
  5. ncbi Amino acid-stimulated Ca2+ oscillations produced by the Ca2+-sensing receptor are mediated by a phospholipase C/inositol 1,4,5-trisphosphate-independent pathway that requires G12, Rho, filamin-A, and the actin cytoskeleton
    Osvaldo Rey
    Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, Department of Medicine, UCLA CURE Digestive Diseases Research Center, David Geffen School of Medicine, University of California at Los Angeles, 90095, USA
    J Biol Chem 280:22875-82. 2005
  6. ncbi G protein-coupled receptor-mediated phosphorylation of the activation loop of protein kinase D: dependence on plasma membrane translocation and protein kinase Cepsilon
    Osvaldo Rey
    Department of Medicine, UCLA CURE Digestive Diseases Research Center and Molecular Biology Institute, David Geffen School of Medicine, University of California Los Angeles, CA 90095, USA
    J Biol Chem 279:34361-72. 2004
  7. ncbi Protein kinase D3 activation and phosphorylation by signaling through G alpha q
    Jingzhen Yuan
    Department of Medicine, David Geffen School of Medicine and Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
    Biochem Biophys Res Commun 335:270-6. 2005
  8. ncbi Activation of protein kinase D3 by signaling through Rac and the alpha subunits of the heterotrimeric G proteins G12 and G13
    Jingzhen Yuan
    Department of Medicine, David Geffen School of Medicine and Molecular Biology Institute, University of California, 900 Veteran Ave, Warren Hall, Rm 11 124, Los Angeles, CA 90095 1786, USA
    Cell Signal 18:1051-62. 2006
  9. ncbi Protein kinase C nu/protein kinase D3 nuclear localization, catalytic activation, and intracellular redistribution in response to G protein-coupled receptor agonists
    Osvaldo Rey
    Department of Medicine, UCLA CURE Digestive Diseases Research Center and Molecular Biology Institute, University of California, Los Angeles, California 90095 1786, USA
    J Biol Chem 278:23773-85. 2003
  10. pmc Sequential protein kinase C (PKC)-dependent and PKC-independent protein kinase D catalytic activation via Gq-coupled receptors: differential regulation of activation loop Ser(744) and Ser(748) phosphorylation
    Rodrigo Jacamo
    Department of Medicine, David Geffen School of Medicine, UCLA, Los Angeles, California 90095, USA
    J Biol Chem 283:12877-87. 2008

Collaborators

Detail Information

Publications24

  1. pmc Negative cross-talk between calcium-sensing receptor and β-catenin signaling systems in colonic epithelium
    Osvaldo Rey
    Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, Department of Medicine, CURE Digestive Diseases Research Center, David Geffen School of Medicine, UCLA, Los Angeles, California 90095, USA
    J Biol Chem 287:1158-67. 2012
    ..These studies demonstrate that signaling pathways emanating from the CaSR control colonic epithelial cell proliferation in vivo and suggest that the mechanism involves regulation of β-catenin phosphorylation...
  2. pmc Extracellular calcium sensing receptor stimulation in human colonic epithelial cells induces intracellular calcium oscillations and proliferation inhibition
    Osvaldo Rey
    Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, Department of Medicine, CURE, Digestive Diseases Research Center, Molecular Biology Institute and Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, University of California at Los Angeles, California 90095 1786, USA
    J Cell Physiol 225:73-83. 2010
    ..Thus, these results indicate that the CaR can function in two signaling modes in human colonic epithelial cells offering a potential link between gastrointestinal responses and food/nutrients uptake and metabolism...
  3. ncbi Requirement of the TRPC1 cation channel in the generation of transient Ca2+ oscillations by the calcium-sensing receptor
    Osvaldo Rey
    Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, 900 Veteran Avenue, Los Angeles, CA 90095, USA
    J Biol Chem 281:38730-7. 2006
    ..We propose a model for the generation of CaR-mediated transient [Ca(2+)](i) oscillations that integrates its stimulation by aromatic amino acids with TRPC1 regulation by PKC and calmodulin...
  4. ncbi The nuclear import of protein kinase D3 requires its catalytic activity
    Osvaldo Rey
    Division of Digestive Diseases, Department of Medicine, CURE Digestive Diseases Research Center and Molecular Biology Institute, David Geffen School of Medicine, University of California, Los Angeles, California 90095 1786, USA
    J Biol Chem 281:5149-57. 2006
    ....
  5. ncbi Amino acid-stimulated Ca2+ oscillations produced by the Ca2+-sensing receptor are mediated by a phospholipase C/inositol 1,4,5-trisphosphate-independent pathway that requires G12, Rho, filamin-A, and the actin cytoskeleton
    Osvaldo Rey
    Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, Department of Medicine, UCLA CURE Digestive Diseases Research Center, David Geffen School of Medicine, University of California at Los Angeles, 90095, USA
    J Biol Chem 280:22875-82. 2005
    ..Our model envisages that Ca(2+) or amino acids stabilize unique CaR conformations that favor coupling to different G proteins and subsequent activation of distinct downstream signaling pathways...
  6. ncbi G protein-coupled receptor-mediated phosphorylation of the activation loop of protein kinase D: dependence on plasma membrane translocation and protein kinase Cepsilon
    Osvaldo Rey
    Department of Medicine, UCLA CURE Digestive Diseases Research Center and Molecular Biology Institute, David Geffen School of Medicine, University of California Los Angeles, CA 90095, USA
    J Biol Chem 279:34361-72. 2004
    ..Based on these and previous results, we propose a model of GPCR-mediated PKD regulation that integrates its changes in distribution, catalytic activity, and multisite phosphorylation...
  7. ncbi Protein kinase D3 activation and phosphorylation by signaling through G alpha q
    Jingzhen Yuan
    Department of Medicine, David Geffen School of Medicine and Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
    Biochem Biophys Res Commun 335:270-6. 2005
    ..Our results indicate that G alpha q activation is sufficient to stimulate sustained PKD3 activation and show that the endogenous G alpha q is a major component in the signaling pathway that mediates bombesin-induced PKD3 activation...
  8. ncbi Activation of protein kinase D3 by signaling through Rac and the alpha subunits of the heterotrimeric G proteins G12 and G13
    Jingzhen Yuan
    Department of Medicine, David Geffen School of Medicine and Molecular Biology Institute, University of California, 900 Veteran Ave, Warren Hall, Rm 11 124, Los Angeles, CA 90095 1786, USA
    Cell Signal 18:1051-62. 2006
    ..Our results indicate that Galpha12/13 and Rac are important components in the signal transduction pathways that mediate bombesin receptor-induced PKD3 activation...
  9. ncbi Protein kinase C nu/protein kinase D3 nuclear localization, catalytic activation, and intracellular redistribution in response to G protein-coupled receptor agonists
    Osvaldo Rey
    Department of Medicine, UCLA CURE Digestive Diseases Research Center and Molecular Biology Institute, University of California, Los Angeles, California 90095 1786, USA
    J Biol Chem 278:23773-85. 2003
    ..Thus, this study demonstrates that the intracellular distribution of PKD isoenzymes are distinct, and suggests that their signaling properties are regulated by differential localization...
  10. pmc Sequential protein kinase C (PKC)-dependent and PKC-independent protein kinase D catalytic activation via Gq-coupled receptors: differential regulation of activation loop Ser(744) and Ser(748) phosphorylation
    Rodrigo Jacamo
    Department of Medicine, David Geffen School of Medicine, UCLA, Los Angeles, California 90095, USA
    J Biol Chem 283:12877-87. 2008
    ..The present studies identify a novel mechanism induced by GPCR activation that leads to late, PKC-independent PKD activation...
  11. ncbi Insulin potentiates Ca2+ signaling and phosphatidylinositol 4,5-bisphosphate hydrolysis induced by Gq protein-coupled receptor agonists through an mTOR-dependent pathway
    Krisztina Kisfalvi
    Unit of Signal Transduction and Gastrointestinal Cancer, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles Center for Ulcer Research and Education, Los Angeles, California 90095 1786, USA
    Endocrinology 148:3246-57. 2007
    ....
  12. doi Protein kinase D isozymes activation and localization during mitosis
    Romeo Papazyan
    Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, 90095 1786, USA
    Exp Cell Res 314:3057-68. 2008
    ..Thus, this study reveals a connection between the PKD isozymes and cell division, suggesting a novel role for this family of serine/threonine kinases...
  13. pmc Amino acid sensing by enteroendocrine STC-1 cells: role of the Na+-coupled neutral amino acid transporter 2
    Steven H Young
    Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90095 1786, USA
    Am J Physiol Cell Physiol 298:C1401-13. 2010
    ....
  14. ncbi Human immunodeficiency virus nucleocapsid protein polymorphisms modulate the infectivity of RNA packaging mutants
    Paul Krogstad
    Department of Pediatrics, University of California, Los Angeles School of Medicine, Los Angeles, California 90095 1732, USA
    Virology 294:282-8. 2002
    ..Conservative amino acid differences between HXB2 and NL4-3 NCs were sufficient to explain the difference in infectivity of viruses carrying the R10A and K11A mutations...
  15. ncbi Neurotensin induces protein kinase C-dependent protein kinase D activation and DNA synthesis in human pancreatic carcinoma cell line PANC-1
    Sushovan Guha
    Department of Medicine, Division of Digestive Diseases, School of Medicine and Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
    Cancer Res 62:1632-40. 2002
    ..Collectively our results demonstrate, for the first time, the existence of a functional PKC/PKD signaling pathway in human ductal pancreatic carcinoma cells and suggest that PKCs mediate the mitogenic signaling process initiated by NT...
  16. ncbi Protein kinase D signaling
    Enrique Rozengurt
    Department of Medicine, UCLA CURE Digestive Diseases Research Center and Molecular Biology Institute, David Geffen School of Medicine, University of California, Los Angeles, California 90095, USA
    J Biol Chem 280:13205-8. 2005
  17. ncbi Oxidative stress induces protein kinase C-mediated activation loop phosphorylation and nuclear redistribution of protein kinase D
    Richard T Waldron
    Division of Digestive Diseases, Department of Medicine, UCLA David Geffen School of Medicine, UCLA CURE Digestive Diseases Research Center and Molecular Biology Institute, UCLA, Los Angeles, California 90095 1786, USA
    J Biol Chem 279:27482-93. 2004
    ..Taken together, these results indicate that PKC-mediated PKD Ser(744) and Ser(748) phosphorylation induced by oxidative stress integrates PKD activation with redistribution to the nucleus...
  18. ncbi Effects of altered expression and localization of cyclophilin A on differentiation of p19 embryonic carcinoma cells
    Robert Chiu
    Dental Research Institute, UCLA School of Dentistry, Los Angeles, California 90095 1668, USA
    Cell Mol Neurobiol 23:929-43. 2003
    ..5. The evidence of enriched CypA and colocalization of RB with CypA in the nucleus of primary adult sensory neurons substantiated the important event of RB-mediated neuronal differentiation of p19 EC cells...
  19. ncbi The C-terminal tail of protein kinase D2 and protein kinase D3 regulates their intracellular distribution
    Romeo Papazyan
    Division of Digestive Diseases, Department of Medicine, CURE Digestive Diseases Research Center and Molecular Biology Institute, David Geffen School of Medicine, University of California at Los Angeles, USA
    Biochem Biophys Res Commun 342:685-9. 2006
    ..Collectively, these data demonstrate that the short C-terminal tail of these kinases plays a critical role in determining their cytoplasmic/nuclear localization...
  20. pmc Protein kinase D mediates mitogenic signaling by Gq-coupled receptors through protein kinase C-independent regulation of activation loop Ser744 and Ser748 phosphorylation
    James Sinnett-Smith
    Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, CURE Digestive Diseases Research Center and Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
    J Biol Chem 284:13434-45. 2009
    ..Thus, our results identify a novel mechanism of G(q)-coupled receptor-induced mitogenesis mediated by sustained PKD activation through a PKC-independent pathway...
  21. ncbi Intracellular redistribution of protein kinase D2 in response to G-protein-coupled receptor agonists
    Osvaldo Rey
    Division of Digestive Diseases, Department of Medicine, UCLA CURE Digestive Diseases Research Center and Molecular Biology Institute, David Geffen School of Medicine, University of California, Los Angeles, 90095 1786, USA
    Biochem Biophys Res Commun 302:817-24. 2003
    ....
  22. pmc CID755673 enhances mitogenic signaling by phorbol esters, bombesin and EGF through a protein kinase D-independent pathway
    Eugenia Torres-Marquez
    Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, CURE, Digestive Diseases Research Center and Molecular Biology Institute, University of California, Los Angeles, CA 90095 1786, USA
    Biochem Biophys Res Commun 391:63-8. 2010
    ....
  23. ncbi Protein kinase D2 potentiates MEK/ERK/RSK signaling, c-Fos accumulation and DNA synthesis induced by bombesin in Swiss 3T3 cells
    James Sinnett-Smith
    Division of Digestive Diseases, Department of Medicine, School of Medicine, CURE Digestive Diseases Research Center and Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
    J Cell Physiol 211:781-90. 2007
    ....
  24. ncbi Vasopressin-induced intracellular redistribution of protein kinase D in intestinal epithelial cells
    Osvaldo Rey
    Division of Digestive Diseases, Department of Medicine, UCLA CURE Digestive Diseases Research Center and Molecular Biology Institute, David Geffen School of Medicine, University of California at Los Angeles, California, USA
    J Cell Physiol 196:483-92. 2003
    ..These findings provide evidence for a mechanism by which PKC coordinates in intestinal epithelial cells the translocation and activation of PKD in response to vasopressin-induced GPCR activation...

Research Grants3

  1. SPATIAL AND TEMPORAL REGULATION OF PROTEIN KINASE D
    Osvaldo Rey; Fiscal Year: 2007
    ..Our hypothesis is that the kinase activity of PKC regulates PKD by coordinating its catalytic activation and intracellular localization. ..
  2. Calcium Sensing Receptor Signaling in the Gastrointestinal Tract
    Osvaldo Rey; Fiscal Year: 2007
    ..We also anticipate that these mechanistic studies will be extended to normal colon epithelial cells and thus provide a robust base for the formulation of an R01 award application. ..