S James Remington

Summary

Affiliation: University of Oregon
Country: USA

Publications

  1. ncbi request reprint Fluorescent proteins: maturation, photochemistry and photophysics
    S James Remington
    Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, OR 97403, USA
    Curr Opin Struct Biol 16:714-21. 2006
  2. ncbi request reprint zFP538, a yellow-fluorescent protein from Zoanthus, contains a novel three-ring chromophore
    S James Remington
    Institute of Molecular Biology, Department of Physics, University of Oregon, Eugene, Oregon 97403 1229, USA
    Biochemistry 44:202-12. 2005
  3. ncbi request reprint Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators
    George T Hanson
    Department of Biology, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    J Biol Chem 279:13044-53. 2004
  4. doi request reprint Structure and mechanism of the photoactivatable green fluorescent protein
    J Nathan Henderson
    Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, Oregon 97403 1229, USA
    J Am Chem Soc 131:4176-7. 2009
  5. pmc Unique interactions between the chromophore and glutamate 16 lead to far-red emission in a red fluorescent protein
    Xiaokun Shu
    Department of Physics, Institute of Molecular Biology, University of Oregon, Eugene, 97403, USA
    Protein Sci 18:460-6. 2009
  6. pmc Re-engineering redox-sensitive green fluorescent protein for improved response rate
    Mark B Cannon
    Department of Chemistry, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    Protein Sci 15:45-57. 2006
  7. ncbi request reprint Energy substrate modulates mitochondrial structure and oxidative capacity in cancer cells
    Rodrigue Rossignol
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon, USA
    Cancer Res 64:985-93. 2004
  8. pmc Systematic replacement of lysine with glutamine and alanine in Escherichia coli malate synthase G: effect on crystallization
    David M Anstrom
    Institute of Molecular Biology, University of Oregon, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:1069-74. 2005
  9. ncbi request reprint Kindling fluorescent protein from Anemonia sulcata: dark-state structure at 1.38 A resolution
    Michael L Quillin
    Department of Physics, Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403, USA
    Biochemistry 44:5774-87. 2005
  10. pmc Atomic resolution structures of Escherichia coli and Bacillus anthracis malate synthase A: comparison with isoform G and implications for structure-based drug discovery
    Jeremy R Lohman
    Department of Physics, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    Protein Sci 17:1935-45. 2008

Research Grants

  1. Development of Fluorescent Protein Biosensors
    S James Remington; Fiscal Year: 2007
  2. Development of Fluorescent Protein Biosensors
    S James Remington; Fiscal Year: 2006
  3. Development of Fluorescent Protein Biosensors
    S James Remington; Fiscal Year: 2005
  4. BIOSENSORS AND DYNAMICS OF GREEN FLUORESCENT PROTEIN
    S James Remington; Fiscal Year: 2004
  5. BIOSENSORS AND DYNAMICS OF GREEN FLUORESCENT PROTEIN
    S James Remington; Fiscal Year: 2003
  6. BIOSENSORS AND DYNAMICS OF GREEN FLUORESCENT PROTEIN
    S James Remington; Fiscal Year: 2002
  7. BIOSENSORS AND DYNAMICS OF GREEN FLUORESCENT PROTEIN
    S James Remington; Fiscal Year: 2001
  8. BIOSENSORS AND DYNAMICS OF GREEN FLUORESCENT PROTEIN
    S James Remington; Fiscal Year: 2000
  9. STRUCTURE, MECHANISM AND REGULATION OF GLYCEROL KINASE
    S James Remington; Fiscal Year: 1993
  10. STRUCTURE, MECHANISM AND REGULATION OF GLYCEROL KINASE
    S James Remington; Fiscal Year: 1992

Collaborators

Detail Information

Publications36

  1. ncbi request reprint Fluorescent proteins: maturation, photochemistry and photophysics
    S James Remington
    Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, OR 97403, USA
    Curr Opin Struct Biol 16:714-21. 2006
    ....
  2. ncbi request reprint zFP538, a yellow-fluorescent protein from Zoanthus, contains a novel three-ring chromophore
    S James Remington
    Institute of Molecular Biology, Department of Physics, University of Oregon, Eugene, Oregon 97403 1229, USA
    Biochemistry 44:202-12. 2005
    ..The diverse and unexpected roles of the side chain at position 66 give new insight into the chemistry of chromophore maturation in the extended family of GFP-like proteins...
  3. ncbi request reprint Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators
    George T Hanson
    Department of Biology, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    J Biol Chem 279:13044-53. 2004
    ..T. Dooley, T. M. Dore, G. Hanson, W. C. Jackson, S. J. Remington, and R. Y. Tsien, submitted for publication), it is shown that the cytosol of HeLa cells is also unusually reducing but somewhat less so than the mitochondrial matrix...
  4. doi request reprint Structure and mechanism of the photoactivatable green fluorescent protein
    J Nathan Henderson
    Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, Oregon 97403 1229, USA
    J Am Chem Soc 131:4176-7. 2009
    ..Additionally, the structures provide insights into the spectroscopic differences between WT and PA-GFP steady-state fluorescence maxima and excited-state proton transfer dynamics...
  5. pmc Unique interactions between the chromophore and glutamate 16 lead to far-red emission in a red fluorescent protein
    Xiaokun Shu
    Department of Physics, Institute of Molecular Biology, University of Oregon, Eugene, 97403, USA
    Protein Sci 18:460-6. 2009
    ..Our findings suggest that significant red shifts might be achieved in other fluorescent proteins using the strategy that led to the discovery of mPlum...
  6. pmc Re-engineering redox-sensitive green fluorescent protein for improved response rate
    Mark B Cannon
    Department of Chemistry, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    Protein Sci 15:45-57. 2006
    ..roGFP1-R12 is most suitable for use in live cells, due to significantly increased reaction rate and increased pI...
  7. ncbi request reprint Energy substrate modulates mitochondrial structure and oxidative capacity in cancer cells
    Rodrigue Rossignol
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon, USA
    Cancer Res 64:985-93. 2004
    ..We compared our finding on HeLa cells with those for nontransformed fibroblasts to help distinguish the regulatory pathways...
  8. pmc Systematic replacement of lysine with glutamine and alanine in Escherichia coli malate synthase G: effect on crystallization
    David M Anstrom
    Institute of Molecular Biology, University of Oregon, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:1069-74. 2005
    ..Glutamine substitutions were found to be more effective than alanine in producing crystals, in support of proposal II. Secondary structure at the site of mutation does not appear to play a major role in determining the rate of success...
  9. ncbi request reprint Kindling fluorescent protein from Anemonia sulcata: dark-state structure at 1.38 A resolution
    Michael L Quillin
    Department of Physics, Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403, USA
    Biochemistry 44:5774-87. 2005
    ....
  10. pmc Atomic resolution structures of Escherichia coli and Bacillus anthracis malate synthase A: comparison with isoform G and implications for structure-based drug discovery
    Jeremy R Lohman
    Department of Physics, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    Protein Sci 17:1935-45. 2008
    ..coli are nearly identical. Considering that inhibitors bind with very similar affinities to both isoforms, MSA is as an excellent platform for high-resolution structural studies and drug discovery efforts...
  11. pmc Excited state proton transfer in the red fluorescent protein mKeima
    J Nathan Henderson
    Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, Oregon 97403 1229, USA
    J Am Chem Soc 131:13212-3. 2009
    ..Thus, excited state proton transfer (ESPT) explains the large Stokes shift. This work unambiguously characterizes green emission from the protonated acylimine chromophore of red fluorescent proteins...
  12. pmc An alternative excited-state proton transfer pathway in green fluorescent protein variant S205V
    Xiaokun Shu
    Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, Oregon 97403 1229, USA
    Protein Sci 16:2703-10. 2007
    ..The results have implications for the detailed mechanism of ESPT and the photocycle of wt-GFP, in particular for the structures of spectroscopically identified intermediates in the cycle...
  13. pmc Structure and proposed mechanism for the pH-sensing Helicobacter pylori chemoreceptor TlpB
    Emily Goers Sweeney
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA
    Structure 20:1177-88. 2012
    ..pylori to sense acid. Our signaling model predicts that protonation events at Asp114, affected by changes in pH, dictate the stability of TlpB through urea binding...
  14. pmc Ultrafast excited-state dynamics in the green fluorescent protein variant S65T/H148D. 1. Mutagenesis and structural studies
    Xiaokun Shu
    Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, Oregon 97403 1229, USA
    Biochemistry 46:12005-13. 2007
    ..4 A), and possibly low-barrier, hydrogen bond between the chromophore hydroxyl and introduced Asp148...
  15. doi request reprint Development of a family of redox-sensitive green fluorescent protein indicators for use in relatively oxidizing subcellular environments
    Jeremy R Lohman
    Institute of Molecular Biology and Departments of Chemistry and Physics, University of Oregon, Eugene, Oregon 97403, USA
    Biochemistry 47:8678-88. 2008
    ....
  16. ncbi request reprint Green fluorescent protein variants as ratiometric dual emission pH sensors. 1. Structural characterization and preliminary application
    George T Hanson
    Department of Chemistry, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    Biochemistry 41:15477-88. 2002
    ..Given their favorable optical characteristics, suitable pK(a)'s for the physiological pH range, and suitability for ratiometric measurements, dual emission GFPs should make excellent probes for studying pH in vivo...
  17. ncbi request reprint The kindling fluorescent protein: a transient photoswitchable marker
    J Nathan Henderson
    Department of Chemistry, University of Oregon, Eugene, Oregon, USA
    Physiology (Bethesda) 21:162-70. 2006
    ..The "kindling fluorescent protein" is a photoactivatable marker with a novel twist: it turns itself off after a selectable period...
  18. pmc The product complex of M. tuberculosis malate synthase revisited
    David M Anstrom
    Institute of Molecular Biology, Department of Chemistry, University of Oregon, Eugene, 97403, USA
    Protein Sci 15:2002-7. 2006
    ..The results should be useful in the design of malate synthase inhibitors...
  19. ncbi request reprint Novel chromophores and buried charges control color in mFruits
    Xiaokun Shu
    Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, Oregon 97403, USA
    Biochemistry 45:9639-47. 2006
    ..pH-dependent spectral shifts of mCherry and mStrawberry appear to result from the titration of Glu 215, although, for mStrawberry, partial cyclization of Thr 66 may contribute at high pH...
  20. pmc Structural basis for reversible photobleaching of a green fluorescent protein homologue
    J Nathan Henderson
    Departments of Chemistry and Physics, and Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA
    Proc Natl Acad Sci U S A 104:6672-7. 2007
    ..The fundamental mechanism appears to be common to all of the photoactivatable and reversibly photoswitchable FPs reported to date...
  21. pmc Structure of the Escherichia coli malate synthase G:pyruvate:acetyl-coenzyme A abortive ternary complex at 1.95 A resolution
    David M Anstrom
    Departments of Chemistry and Physics, University of Oregon, Eugene, Oregon 97403, USA
    Protein Sci 12:1822-32. 2003
    ..The results described in this study add insight into the mechanism of catalysis and may be useful for the design of inhibitory compounds as possible antimicrobial agents...
  22. pmc Green fluorescent protein: a perspective
    S James Remington
    Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, Oregon 97403 1229, USA
    Protein Sci 20:1509-19. 2011
    ..Structure-function relationships in photoswitchable fluorescent proteins and nonfluorescent chromoproteins are also briefly covered...
  23. pmc Crystal structures and mutational analysis of amFP486, a cyan fluorescent protein from Anemonia majano
    J Nathan Henderson
    Department of Physics and Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA
    Proc Natl Acad Sci U S A 102:12712-7. 2005
    ....
  24. ncbi request reprint A replicating module as the unit of mitochondrial structure and functioning
    Roderick A Capaldi
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    Biochim Biophys Acta 1555:192-5. 2002
    ..This leads us to propose that a replicating module is the repeating unit of mitochondrial structure. Studies to examine heterogeneity of functioning within the organelle mass are briefly reviewed...
  25. pmc Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging
    Hui Wang Ai
    Department of Chemistry, University of Alberta, Edmonton, AB, Canada T6G 2G2
    Biochem J 400:531-40. 2006
    ..85) makes it particularly suitable as a replacement for ECFP (enhanced CFP) or Cerulean as a FRET (fluorescence resonance energy transfer) donor to either a yellow or orange FP acceptor...
  26. ncbi request reprint Green fluorescent protein variants as ratiometric dual emission pH sensors. 2. Excited-state dynamics
    Tim B McAnaney
    Department of Chemistry, Stanford University, Stanford, CA 94305 5080, USA
    Biochemistry 41:15489-94. 2002
    ..At low pH, excited-state proton transfer is slowed to the point where it is no longer rate limiting...
  27. pmc Expression and characterization of a redox-sensing green fluorescent protein (reduction-oxidation-sensitive green fluorescent protein) in Arabidopsis
    Keni Jiang
    Department of Plant and Microbial Biology, University of California, Berkeley, 94720, USA
    Plant Physiol 141:397-403. 2006
    ..The data show that roGFP is redox sensitive in plant cells and that this sensor makes it possible to monitor, in real time, dynamic changes in redox in vivo...
  28. ncbi request reprint Imaging dynamic redox changes in mammalian cells with green fluorescent protein indicators
    Colette T Dooley
    Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, California 92093 0647, USA
    J Biol Chem 279:22284-93. 2004
    ..g. epidermal growth factor and lysophosphatidic acid...
  29. pmc Organelle redox of CF and CFTR-corrected airway epithelia
    Christian Schwarzer
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720 3200, USA
    Free Radic Biol Med 43:300-16. 2007
    ..These quantitative estimates of organelle redox potentials combined with apical and cell measurements using small-molecule couples confirmed there were no differences in the redox properties of CF and CFTR-corrected cells...
  30. ncbi request reprint The permeability transition pore signals apoptosis by directing Bax translocation and multimerization
    FrancesaA De Giorgi
    European Institute of Chemistry and Biology, and INSERM E 9929, Victor Segalen Bordeaux 2 University, 33076 Bordeaux Cedex, France
    FASEB J 16:607-9. 2002
    ..We conclude that the PTP is not itself a component of the Cyt.c release machinery, but that it acts indirectly by signaling Bax translocation and multimerization...
  31. ncbi request reprint Synthesis and properties of the chromophore of the asFP595 chromoprotein from Anemonia sulcata
    Ilia V Yampolsky
    Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho Maklaya 16 10, 117997 Moscow, Russia
    Biochemistry 44:5788-93. 2005
    ....
  32. ncbi request reprint Green fluorescent protein variants as ratiometric dual emission pH sensors. 3. Temperature dependence of proton transfer
    Tim B McAnaney
    Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
    Biochemistry 44:8701-11. 2005
    ....
  33. ncbi request reprint Effects of mutations and truncations on the kinetic behavior of IIAGlc, a phosphocarrier and regulatory protein of the phosphoenolpyruvate phosphotransferase system of Escherichia coli
    Norman D Meadow
    Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218, USA
    J Biol Chem 281:11450-5. 2006
    ..The results support the hypothesis (Wang, G., Peterkofsky, A., and Clore, G. M. (2000) J. Biol. Chem. 275, 39811-39814) that the N-terminal 18-residue domain "docks" IIAGlc to the lipid bilayer of membranes containing IICBGlc...
  34. ncbi request reprint Ultrafast excited-state dynamics in the green fluorescent protein variant S65T/H148D. 3. Short- and long-time dynamics of the excited-state proton transfer
    Pavel Leiderman
    Raymond and Beverly Sackler Faculty of Exact Sciences, School of Chemistry, Tel Aviv University, Tel Aviv 69978, Israel
    Biochemistry 46:12026-36. 2007
    ..The spectroscopic results are discussed on the basis of the detailed X-ray structure of the mutant published in the preceding paper (Shu et al.)...
  35. pmc Ultrafast excited-state dynamics in the green fluorescent protein variant S65T/H148D. 2. Unusual photophysical properties
    Xinghua Shi
    Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
    Biochemistry 46:12014-25. 2007
    ..It is speculated that two different orientations of the Asp introduced at position 148, not distinguishable by chromatography, mass spectrometry, or X-ray crystallography, give rise to the two functionally distinct populations...
  36. ncbi request reprint Negotiating the speed bumps to fluorescence
    S James Remington
    Nat Biotechnol 20:28-9. 2002

Research Grants16

  1. Development of Fluorescent Protein Biosensors
    S James Remington; Fiscal Year: 2007
    ..abstract_text> ..
  2. Development of Fluorescent Protein Biosensors
    S James Remington; Fiscal Year: 2006
    ..abstract_text> ..
  3. Development of Fluorescent Protein Biosensors
    S James Remington; Fiscal Year: 2005
    ..abstract_text> ..
  4. BIOSENSORS AND DYNAMICS OF GREEN FLUORESCENT PROTEIN
    S James Remington; Fiscal Year: 2004
    ..The structural basis for these four transformations will be determined on flash-frozen crystals by crystallographic techniques for potential biological and/or commercial uses. ..
  5. BIOSENSORS AND DYNAMICS OF GREEN FLUORESCENT PROTEIN
    S James Remington; Fiscal Year: 2003
    ..The structural basis for these four transformations will be determined on flash-frozen crystals by crystallographic techniques for potential biological and/or commercial uses. ..
  6. BIOSENSORS AND DYNAMICS OF GREEN FLUORESCENT PROTEIN
    S James Remington; Fiscal Year: 2002
    ..The structural basis for these four transformations will be determined on flash-frozen crystals by crystallographic techniques for potential biological and/or commercial uses. ..
  7. BIOSENSORS AND DYNAMICS OF GREEN FLUORESCENT PROTEIN
    S James Remington; Fiscal Year: 2001
    ..The structural basis for these four transformations will be determined on flash-frozen crystals by crystallographic techniques for potential biological and/or commercial uses. ..
  8. BIOSENSORS AND DYNAMICS OF GREEN FLUORESCENT PROTEIN
    S James Remington; Fiscal Year: 2000
    ..The structural basis for these four transformations will be determined on flash-frozen crystals by crystallographic techniques for potential biological and/or commercial uses. ..
  9. STRUCTURE, MECHANISM AND REGULATION OF GLYCEROL KINASE
    S James Remington; Fiscal Year: 1993
    ..effectors of the activity modulate the structure in similar or different ways? What is the nature of a phosphorylation dependent protein-protein interaction? Does glycerol kinase have structural features in common with other ATPases?..
  10. STRUCTURE, MECHANISM AND REGULATION OF GLYCEROL KINASE
    S James Remington; Fiscal Year: 1992
    ..effectors of the activity modulate the structure in similar or different ways? What is the nature of a phosphorylation dependent protein-protein interaction? Does glycerol kinase have structural features in common with other ATPases?..