Research Topics
| S James RemingtonSummaryAffiliation: University of Oregon Country: USA Publications
Research Grants
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Detail Information
Publications
Fluorescent proteins: maturation, photochemistry and photophysicsS James Remington
Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, OR 97403, USA
Curr Opin Struct Biol 16:714-21. 2006....- zFP538, a yellow-fluorescent protein from Zoanthus, contains a novel three-ring chromophoreS James Remington
Institute of Molecular Biology, Department of Physics, University of Oregon, Eugene, Oregon 97403 1229, USA
Biochemistry 44:202-12. 2005..The diverse and unexpected roles of the side chain at position 66 give new insight into the chemistry of chromophore maturation in the extended family of GFP-like proteins... - Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicatorsGeorge T Hanson
Department of Biology, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403-1229, USA
J Biol Chem 279:13044-53. 2004..T. Dooley, T. M. Dore, G. Hanson, W. C. Jackson, S. J. Remington, and R. Y. Tsien, submitted for publication), it is shown that the cytosol of HeLa cells is also unusually reducing but somewhat less so than the mitochondrial matrix... 
Structure and mechanism of the photoactivatable green fluorescent proteinJ Nathan Henderson
Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, Oregon 97403 1229, USA
J Am Chem Soc 131:4176-7. 2009..Additionally, the structures provide insights into the spectroscopic differences between WT and PA-GFP steady-state fluorescence maxima and excited-state proton transfer dynamics...
Unique interactions between the chromophore and glutamate 16 lead to far-red emission in a red fluorescent proteinXiaokun Shu
Department of Physics, Institute of Molecular Biology, University of Oregon, Eugene, 97403, USA
Protein Sci 18:460-6. 2009..Our findings suggest that significant red shifts might be achieved in other fluorescent proteins using the strategy that led to the discovery of mPlum...- Re-engineering redox-sensitive green fluorescent protein for improved response rateMark B Cannon
Department of Chemistry, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403-1229, USA
Protein Sci 15:45-57. 2006..roGFP1-R12 is most suitable for use in live cells, due to significantly increased reaction rate and increased pI... - Energy substrate modulates mitochondrial structure and oxidative capacity in cancer cellsRodrigue Rossignol
Institute of Molecular Biology, University of Oregon, Eugene, Oregon, USA
Cancer Res 64:985-93. 2004..We compared our finding on HeLa cells with those for nontransformed fibroblasts to help distinguish the regulatory pathways... - Systematic replacement of lysine with glutamine and alanine in Escherichia coli malate synthase G: effect on crystallizationDavid M Anstrom
Institute of Molecular Biology, University of Oregon, USA
Acta Crystallogr Sect F Struct Biol Cryst Commun 61:1069-74. 2005..Glutamine substitutions were found to be more effective than alanine in producing crystals, in support of proposal II. Secondary structure at the site of mutation does not appear to play a major role in determining the rate of success... - Kindling fluorescent protein from Anemonia sulcata: dark-state structure at 1.38 A resolutionMichael L Quillin
Department of Physics, Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403, USA
Biochemistry 44:5774-87. 2005.... - Atomic resolution structures of Escherichia coli and Bacillus anthracis malate synthase A: comparison with isoform G and implications for structure-based drug discoveryJeremy R Lohman
Department of Physics, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
Protein Sci 17:1935-45. 2008..coli are nearly identical. Considering that inhibitors bind with very similar affinities to both isoforms, MSA is as an excellent platform for high-resolution structural studies and drug discovery efforts... - Excited state proton transfer in the red fluorescent protein mKeimaJ Nathan Henderson
Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, Oregon 97403 1229, USA
J Am Chem Soc 131:13212-3. 2009..Thus, excited state proton transfer (ESPT) explains the large Stokes shift. This work unambiguously characterizes green emission from the protonated acylimine chromophore of red fluorescent proteins... - An alternative excited-state proton transfer pathway in green fluorescent protein variant S205VXiaokun Shu
Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, Oregon 97403 1229, USA
Protein Sci 16:2703-10. 2007..The results have implications for the detailed mechanism of ESPT and the photocycle of wt-GFP, in particular for the structures of spectroscopically identified intermediates in the cycle... - Structure and proposed mechanism for the pH-sensing Helicobacter pylori chemoreceptor TlpBEmily Goers Sweeney
Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA
Structure 20:1177-88. 2012..pylori to sense acid. Our signaling model predicts that protonation events at Asp114, affected by changes in pH, dictate the stability of TlpB through urea binding... - Ultrafast excited-state dynamics in the green fluorescent protein variant S65T/H148D. 1. Mutagenesis and structural studiesXiaokun Shu
Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, Oregon 97403 1229, USA
Biochemistry 46:12005-13. 2007..4 A), and possibly low-barrier, hydrogen bond between the chromophore hydroxyl and introduced Asp148... - Development of a family of redox-sensitive green fluorescent protein indicators for use in relatively oxidizing subcellular environmentsJeremy R Lohman
Institute of Molecular Biology and Departments of Chemistry and Physics, University of Oregon, Eugene, Oregon 97403, USA
Biochemistry 47:8678-88. 2008.... - Green fluorescent protein variants as ratiometric dual emission pH sensors. 1. Structural characterization and preliminary applicationGeorge T Hanson
Department of Chemistry, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403-1229, USA
Biochemistry 41:15477-88. 2002..Given their favorable optical characteristics, suitable pK(a)'s for the physiological pH range, and suitability for ratiometric measurements, dual emission GFPs should make excellent probes for studying pH in vivo... - The kindling fluorescent protein: a transient photoswitchable markerJ Nathan Henderson
Department of Chemistry, University of Oregon, Eugene, Oregon, USA
Physiology (Bethesda) 21:162-70. 2006..The "kindling fluorescent protein" is a photoactivatable marker with a novel twist: it turns itself off after a selectable period... - The product complex of M. tuberculosis malate synthase revisitedDavid M Anstrom
Institute of Molecular Biology, Department of Chemistry, University of Oregon, Eugene, 97403, USA
Protein Sci 15:2002-7. 2006..The results should be useful in the design of malate synthase inhibitors... - Novel chromophores and buried charges control color in mFruitsXiaokun Shu
Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, Oregon 97403, USA
Biochemistry 45:9639-47. 2006..pH-dependent spectral shifts of mCherry and mStrawberry appear to result from the titration of Glu 215, although, for mStrawberry, partial cyclization of Thr 66 may contribute at high pH... - Structural basis for reversible photobleaching of a green fluorescent protein homologueJ Nathan Henderson
Departments of Chemistry and Physics, and Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA
Proc Natl Acad Sci U S A 104:6672-7. 2007..The fundamental mechanism appears to be common to all of the photoactivatable and reversibly photoswitchable FPs reported to date... - Structure of the Escherichia coli malate synthase G:pyruvate:acetyl-coenzyme A abortive ternary complex at 1.95 A resolutionDavid M Anstrom
Departments of Chemistry and Physics, University of Oregon, Eugene, Oregon 97403, USA
Protein Sci 12:1822-32. 2003..The results described in this study add insight into the mechanism of catalysis and may be useful for the design of inhibitory compounds as possible antimicrobial agents... - Green fluorescent protein: a perspectiveS James Remington
Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, Oregon 97403 1229, USA
Protein Sci 20:1509-19. 2011..Structure-function relationships in photoswitchable fluorescent proteins and nonfluorescent chromoproteins are also briefly covered... - Crystal structures and mutational analysis of amFP486, a cyan fluorescent protein from Anemonia majanoJ Nathan Henderson
Department of Physics and Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA
Proc Natl Acad Sci U S A 102:12712-7. 2005.... - A replicating module as the unit of mitochondrial structure and functioningRoderick A Capaldi
Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
Biochim Biophys Acta 1555:192-5. 2002..This leads us to propose that a replicating module is the repeating unit of mitochondrial structure. Studies to examine heterogeneity of functioning within the organelle mass are briefly reviewed... - Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imagingHui-wang Ai
Department of Chemistry, University of Alberta, Edmonton, AB, Canada T6G 2G2
Biochem J 400:531-40. 2006..85) makes it particularly suitable as a replacement for ECFP (enhanced CFP) or Cerulean as a FRET (fluorescence resonance energy transfer) donor to either a yellow or orange FP acceptor... - Green fluorescent protein variants as ratiometric dual emission pH sensors. 2. Excited-state dynamicsTim B McAnaney
Department of Chemistry, Stanford University, Stanford, CA 94305-5080, USA
Biochemistry 41:15489-94. 2002..At low pH, excited-state proton transfer is slowed to the point where it is no longer rate limiting... - Expression and characterization of a redox-sensing green fluorescent protein (reduction-oxidation-sensitive green fluorescent protein) in ArabidopsisKeni Jiang
Department of Plant and Microbial Biology, University of California, Berkeley, 94720, USA
Plant Physiol 141:397-403. 2006..The data show that roGFP is redox sensitive in plant cells and that this sensor makes it possible to monitor, in real time, dynamic changes in redox in vivo... - Imaging dynamic redox changes in mammalian cells with green fluorescent protein indicatorsColette T Dooley
Department of Chemistry and Biochemistry, University of California-San Diego, La Jolla, California 92093-0647, USA
J Biol Chem 279:22284-93. 2004..g. epidermal growth factor and lysophosphatidic acid... - Organelle redox of CF and CFTR-corrected airway epitheliaChristian Schwarzer
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720 3200, USA
Free Radic Biol Med 43:300-16. 2007..These quantitative estimates of organelle redox potentials combined with apical and cell measurements using small-molecule couples confirmed there were no differences in the redox properties of CF and CFTR-corrected cells... - The permeability transition pore signals apoptosis by directing Bax translocation and multimerizationFrancesaA De Giorgi
European Institute of Chemistry and Biology, and INSERM E.9929, Victor Segalen-Bordeaux 2 University, 33076 Bordeaux Cedex, France
FASEB J 16:607-9. 2002..We conclude that the PTP is not itself a component of the Cyt.c release machinery, but that it acts indirectly by signaling Bax translocation and multimerization... - Synthesis and properties of the chromophore of the asFP595 chromoprotein from Anemonia sulcataIlia V Yampolsky
Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia
Biochemistry 44:5788-93. 2005.... - Green fluorescent protein variants as ratiometric dual emission pH sensors. 3. Temperature dependence of proton transferTim B McAnaney
Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA
Biochemistry 44:8701-11. 2005.... - Effects of mutations and truncations on the kinetic behavior of IIAGlc, a phosphocarrier and regulatory protein of the phosphoenolpyruvate phosphotransferase system of Escherichia coliNorman D Meadow
Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218, USA
J Biol Chem 281:11450-5. 2006..The results support the hypothesis (Wang, G., Peterkofsky, A., and Clore, G. M. (2000) J. Biol. Chem. 275, 39811-39814) that the N-terminal 18-residue domain "docks" IIAGlc to the lipid bilayer of membranes containing IICBGlc... - Ultrafast excited-state dynamics in the green fluorescent protein variant S65T/H148D. 3. Short- and long-time dynamics of the excited-state proton transferPavel Leiderman
Raymond and Beverly Sackler Faculty of Exact Sciences, School of Chemistry, Tel Aviv University, Tel Aviv 69978, Israel
Biochemistry 46:12026-36. 2007..The spectroscopic results are discussed on the basis of the detailed X-ray structure of the mutant published in the preceding paper (Shu et al.)... - Ultrafast excited-state dynamics in the green fluorescent protein variant S65T/H148D. 2. Unusual photophysical propertiesXinghua Shi
Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
Biochemistry 46:12014-25. 2007..It is speculated that two different orientations of the Asp introduced at position 148, not distinguishable by chromatography, mass spectrometry, or X-ray crystallography, give rise to the two functionally distinct populations... - Negotiating the speed bumps to fluorescenceS James Remington
Nat Biotechnol 20:28-9. 2002
Research Grants
- Development of Fluorescent Protein BiosensorsS James Remington; Fiscal Year: 2007..abstract_text> ..
- BIOSENSORS AND DYNAMICS OF GREEN FLUORESCENT PROTEINS James Remington; Fiscal Year: 2004..The structural basis for these four transformations will be determined on flash-frozen crystals by crystallographic techniques for potential biological and/or commercial uses. ..
- STRUCTURE, MECHANISM AND REGULATION OF GLYCEROL KINASES James Remington; Fiscal Year: 1993..effectors of the activity modulate the structure in similar or different ways? What is the nature of a phosphorylation dependent protein-protein interaction? Does glycerol kinase have structural features in common with other ATPases?..