May Reed

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. ncbi Impaired migration, integrin function, and actin cytoskeletal organization in dermal fibroblasts from a subset of aged human donors
    M J Reed
    Division of Gerontology and Geriatric Medicine, Box 359755, Department of Medicine, University of Washington, Seattle, WA 98104, USA
    Mech Ageing Dev 122:1203-20. 2001
  2. ncbi Nitric oxide effects on the function of aged cells ex vivo and in vivo
    May J Reed
    Department of Medicine, University of Washington, Harborview Medical Center, Seattle, WA 98104, USA
    In Vivo 22:673-9. 2008
  3. ncbi Endothelial precursor cells
    May J Reed
    Department of Medicine, University of Washington, Harborview Medical Center, 300 Ninth Avenue, Seattle, WA, USA
    Stem Cell Rev 3:218-25. 2007
  4. ncbi Culture of murine aortic explants in 3-dimensional extracellular matrix: a novel, miniaturized assay of angiogenesis in vitro
    May J Reed
    Department of Medicine, University of Washington, Harborview Medical Center, Box 359625, 300 Ninth Avenue, Seattle, WA 98104, USA
    Microvasc Res 73:248-52. 2007
  5. ncbi Age-related differences in repair of dermal wounds and myocardial infarcts attenuate during the later stages of healing
    May J Reed
    Department of Medicine, University of Washington, Harborview Medical Center, 300 Ninth Avenue, Seattle, WA 98104, USA
    In Vivo 20:801-6. 2006
  6. ncbi The effects of aging on tumor growth and angiogenesis are tumor-cell dependent
    May J Reed
    Department of Medicine, University of Washington, Harborview Medical Center, Seattle, WA 98104, USA
    Int J Cancer 120:753-60. 2007
  7. ncbi Enhanced angiogenesis characteristic of SPARC-null mice disappears with age
    May J Reed
    Department of Medicine, The University of Washington, Seattle, WA 98104, USA
    J Cell Physiol 204:800-7. 2005
  8. ncbi Miniaturized assays of angiogenesis in vitro
    May J Reed
    Department of Medicine, University of Washington, Harborview Medical Center, Seattle, WA, USA
    Methods Mol Biol 843:87-98. 2012
  9. ncbi Impaired angiogenesis in the aged
    May J Reed
    Department of Medicine, Division of Gerontology and Geriatric Medicine, University of Washington, Seattle, WA 98104, USA
    Sci Aging Knowledge Environ 2004:pe7. 2004
  10. ncbi Impaired angiogenesis in aging is associated with alterations in vessel density, matrix composition, inflammatory response, and growth factor expression
    Eman Sadoun
    Division of Gerontology and Geriatric Medicine, Department of Medicine, University of Washington, Seattle, Washington 98104, USA
    J Histochem Cytochem 51:1119-30. 2003

Research Grants

  1. Aging and the Microvasculature
    May Reed; Fiscal Year: 2006
  2. AGING AND ENDOTHELIAL CELL FUNCTION
    May Reed; Fiscal Year: 2003
  3. AGING AND ENDOTHELIAL CELL FUNCTION
    May Reed; Fiscal Year: 2009

Collaborators

Detail Information

Publications23

  1. ncbi Impaired migration, integrin function, and actin cytoskeletal organization in dermal fibroblasts from a subset of aged human donors
    M J Reed
    Division of Gerontology and Geriatric Medicine, Box 359755, Department of Medicine, University of Washington, Seattle, WA 98104, USA
    Mech Ageing Dev 122:1203-20. 2001
    ....
  2. ncbi Nitric oxide effects on the function of aged cells ex vivo and in vivo
    May J Reed
    Department of Medicine, University of Washington, Harborview Medical Center, Seattle, WA 98104, USA
    In Vivo 22:673-9. 2008
    ..Angiogenesis is impaired in most aged tissues. Accordingly, there is great interest in interventions that improve the ability of aged cells to undergo blood vessel formation and subsequent tissue repair...
  3. ncbi Endothelial precursor cells
    May J Reed
    Department of Medicine, University of Washington, Harborview Medical Center, 300 Ninth Avenue, Seattle, WA, USA
    Stem Cell Rev 3:218-25. 2007
    ..We then review the limited data on features of EPCs that are known to be altered in aging and might better define their clinical utility in older persons...
  4. ncbi Culture of murine aortic explants in 3-dimensional extracellular matrix: a novel, miniaturized assay of angiogenesis in vitro
    May J Reed
    Department of Medicine, University of Washington, Harborview Medical Center, Box 359625, 300 Ninth Avenue, Seattle, WA 98104, USA
    Microvasc Res 73:248-52. 2007
    ..The MRSG assay, which combines low volume, physically robust gels in conjunction with mouse aortic segments, may prove to be a highly useful tool in studies of the process and control of vascular growth...
  5. ncbi Age-related differences in repair of dermal wounds and myocardial infarcts attenuate during the later stages of healing
    May J Reed
    Department of Medicine, University of Washington, Harborview Medical Center, 300 Ninth Avenue, Seattle, WA 98104, USA
    In Vivo 20:801-6. 2006
    ..Late stage healing of dermal wounds and myocardial infarcts in rodents was examined to determine if aged animals "catch up" to their younger counterparts...
  6. ncbi The effects of aging on tumor growth and angiogenesis are tumor-cell dependent
    May J Reed
    Department of Medicine, University of Washington, Harborview Medical Center, Seattle, WA 98104, USA
    Int J Cancer 120:753-60. 2007
    ..These data demonstrate that tumor-cell specific features determine the effect of aging on tumor growth and angiogenesis...
  7. ncbi Enhanced angiogenesis characteristic of SPARC-null mice disappears with age
    May J Reed
    Department of Medicine, The University of Washington, Seattle, WA 98104, USA
    J Cell Physiol 204:800-7. 2005
    ..However, the influence of aging on these processes may be reversed, in part, by growth factor stimulation...
  8. ncbi Miniaturized assays of angiogenesis in vitro
    May J Reed
    Department of Medicine, University of Washington, Harborview Medical Center, Seattle, WA, USA
    Methods Mol Biol 843:87-98. 2012
    ..The chapter focuses on explants derived from mice and use of a miniaturized format that permits efficient utilization of reagents, ease of processing, rapid analysis, and conventional imaging...
  9. ncbi Impaired angiogenesis in the aged
    May J Reed
    Department of Medicine, Division of Gerontology and Geriatric Medicine, University of Washington, Seattle, WA 98104, USA
    Sci Aging Knowledge Environ 2004:pe7. 2004
    ..This Perspective describes many of the myriad components of the angiogenic response that are altered with age. In addition, the impact of impaired angiogenesis on wound healing, vascular disease, and cancer in the aged is discussed...
  10. ncbi Impaired angiogenesis in aging is associated with alterations in vessel density, matrix composition, inflammatory response, and growth factor expression
    Eman Sadoun
    Division of Gerontology and Geriatric Medicine, Department of Medicine, University of Washington, Seattle, Washington 98104, USA
    J Histochem Cytochem 51:1119-30. 2003
    ..6 x 10(3) +/- 7.3 x 10(3)) to 19d (34.9 x 10(3) +/- 17 x 10(3)). We conclude that angiogenesis in aging is not merely delayed, but is altered due to multiple impairments...
  11. ncbi Inhibition of TIMP1 enhances angiogenesis in vivo and cell migration in vitro
    May J Reed
    Division of Gerontology and Geriatric Medicine and Department of Medicine, University of Washington, Seattle, WA 98104, USA
    Microvasc Res 65:9-17. 2003
    ..We propose that blocking TIMP1 improves angiogenesis by increasing cell motility during fibrovascular invasion...
  12. ncbi Matricellular homologs in the foreign body response: hevin suppresses inflammation, but hevin and SPARC together diminish angiogenesis
    Thomas H Barker
    Hope Heart Program, Benaroya Research Institute at Virginia Mason, 1201 Ninth Ave, Seattle, WA 98101, USA
    Am J Pathol 166:923-33. 2005
    ..These results provide important information for further development of implant technology...
  13. ncbi Cleavage of the matricellular protein SPARC by matrix metalloproteinase 3 produces polypeptides that influence angiogenesis
    E Helene Sage
    Department of Vascular Biology, The Hope Heart Institute, Seattle, Washington 98104, USA
    J Biol Chem 278:37849-57. 2003
    ..One of these peptides containing KHGK also demonstrated a concentration-dependent effect on angiogenesis...
  14. ncbi Compromised production of extracellular matrix in mice lacking secreted protein, acidic and rich in cysteine (SPARC) leads to a reduced foreign body reaction to implanted biomaterials
    Pauli Puolakkainen
    Department of Vascular Biology, The Hope Heart Institute, University of Washington, Seattle, Washington 98104, USA
    Am J Pathol 162:627-35. 2003
    ..The altered ECM resulting in decreased capsular thickness, indicative of an altered foreign body reaction in SPARC-null mice, implicates SPARC as an important modulator of the encapsulation of implanted biomaterials...
  15. ncbi Collagen extracts derived from young and aged mice demonstrate different structural properties and cellular effects in three-dimensional gels
    Mamatha Damodarasamy
    Department of Medicine, Harborview Medical Center, University of Washington, Box 359625, 325 Ninth Avenue, Seattle, WA 98104, USA
    J Gerontol A Biol Sci Med Sci 65:209-18. 2010
    ..In summary, collagen extracted from young and aged mice is an effective means to examine the influence of aging on functional properties of ECM that are relevant in vivo...
  16. ncbi Inhibited angiogenesis in aging: a role for TIMP-2
    Teruhiko Koike
    Department of Medicine, University of Washington, Seattle, Washington 98104, USA
    J Gerontol A Biol Sci Med Sci 58:B798-805. 2003
    ..Collectively, our studies indicate that elevated levels of TIMP-2 modulate decreased angiogenesis in aged tissues, most likely via TIMP-2-mediated inhibition of MMP-2 and MT1-MMP...
  17. ncbi SPARC-null mice exhibit accelerated cutaneous wound closure
    Amy D Bradshaw
    Department of Vascular Biology, The Hope Heart Institute, Seattle, Washington 98104, USA
    J Histochem Cytochem 50:1-10. 2002
    ..We propose that the accelerated wound closure seen in SPARC-null dermis results from its decreased collagen content, a condition contributing to enhanced contractibility...
  18. ncbi MT1-MMP, but not secreted MMPs, influences the migration of human microvascular endothelial cells in 3-dimensional collagen gels
    Teruhiko Koike
    Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan
    J Cell Biochem 86:748-58. 2002
    ..Collectively, our data indicate that MT1-MMP contributes significantly to the movement of hmECs through 3D collagen, in contrast to secretory-type MMPs-1, -2, -9, and -13, which are not critical for this movement...
  19. ncbi Wound repair in aging. A review
    May J Reed
    Division of Geriatric Medicine, Department of Medicine, University of Washington, Seattle, WA, USA
    Methods Mol Med 78:217-37. 2003
  20. ncbi Elongation and secretion of tissue inhibitor of metalloproteinases 1 by human microvascular endothelial cells cultured in collagen gels is stimulated by mitomycin c
    Margaret A Hamner
    Division of Gerontology and Geriatric Medicine, Department of Medicine, University of Washington, Seattle, WA 98104-2499, USA
    Endothelium 12:97-101. 2005
    ..These results illustrate that antiproliferative compounds should be used with caution in studies of MMP regulation by ECs...
  21. ncbi SPARC-thrombospondin-2-double-null mice exhibit enhanced cutaneous wound healing and increased fibrovascular invasion of subcutaneous polyvinyl alcohol sponges
    Pauli A Puolakkainen
    Hope Heart Program, Benaroya Research Institute at Virginia Mason, 1201 9th Ave, Seattle, WA 98101, USA
    J Histochem Cytochem 53:571-81. 2005
    ..SPARC and TSP-2 therefore perform similar functions in the regulation of cutaneous wound healing, but fine-tuning with respect to ECM production and remodeling could account for the enhanced response seen in ST-double-null mice...
  22. ncbi Aging and angiogenesis
    Jay M Edelberg
    Department of Medicine, Weill Medical College of Cornell University, New York, NY 10021, USA
    Front Biosci 8:s1199-209. 2003
    ....
  23. ncbi Defects in activation of nitric oxide synthases occur during delayed angiogenesis in aging
    Mary H M Bach
    Department of Medicine, Division of Gerontology and Geriatric Medicine, Harborview Medical Center Research and Training Building, School of Medicine, University of Washington, Box 359755, 325 9th Ave, Seattle, WA 98104-2499, USA
    Mech Ageing Dev 126:467-73. 2005
    ..We propose that the subsequent lack of NO contributes to impaired angiogenesis in aging...

Research Grants10

  1. Aging and the Microvasculature
    May Reed; Fiscal Year: 2006
    ..The results will define the key points of deficient microvascular growth in the aged and also develop the microvessel sprout model into a highly useful tool for studies of vascular growth in general. ..
  2. AGING AND ENDOTHELIAL CELL FUNCTION
    May Reed; Fiscal Year: 2003
    ..4. Determine the effects of age and VEGF on: expression and function of MMP1/TIMP1 and alpha2beta1, and the migration of microvascular EC during angiogenesis in vivo. ..
  3. AGING AND ENDOTHELIAL CELL FUNCTION
    May Reed; Fiscal Year: 2009
    ..The elucidation of mechanisms that regulate MMP activity in aged cells and tissues will assist the development of therapies to improve wound repair and revascularization of ischemic organs. ..