George H Reed

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. pmc Radical reactions of thiamin pyrophosphate in 2-oxoacid oxidoreductases
    George H Reed
    Department of Biochemistry, University of Wisconsin Madison, 1710 University Avenue, Madison, WI 53726, USA
    Biochim Biophys Acta 1824:1291-8. 2012
  2. pmc Identification of the substrate radical intermediate derived from ethanolamine during catalysis by ethanolamine ammonia-lyase
    Gunes Bender
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53726, USA
    Biochemistry 47:11360-6. 2008
  3. pmc Analysis of the Cob(II)alamin-5'-deoxy-3',4'-anhydroadenosyl radical triplet spin system in the active site of diol dehydrase
    Steven O Mansoorabadi
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53726 4087, USA
    Biochemistry 45:14362-70. 2006
  4. pmc Characterization of a succinyl-CoA radical-cob(II)alamin spin triplet intermediate in the reaction catalyzed by adenosylcobalamin-dependent methylmalonyl-CoA mutase
    Steven O Mansoorabadi
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53726 4087, USA
    Biochemistry 44:3153-8. 2005
  5. pmc Reaction of AdoMet with ThiC generates a backbone free radical
    N Cecilia Martinez-Gomez
    Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biochemistry 48:217-9. 2009
  6. pmc Radical triplets and suicide inhibition in reactions of 4-thia-D- and 4-thia-L-lysine with lysine 5,6-aminomutase
    Kuo Hsiang Tang
    Department of Biochemistry, University of Wisconsin Madison, 1710 University Avenue, Madison, Wisconsin 53726, USA
    Biochemistry 48:8151-60. 2009
  7. pmc Probing interactions from solvent-exchangeable protons and monovalent cations with the 1,2-propanediol-1-yl radical intermediate in the reaction of dioldehydrase
    Phillip A Schwartz
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53726, USA
    Protein Sci 16:1157-64. 2007
  8. ncbi request reprint Free radical mechanisms in enzymology
    Perry A Frey
    Department of Biochemistry, University of Wisconsin Madison, 1710 University Avenue, Madison, Wisconsin 53726, USA
    Chem Rev 106:3302-16. 2006
  9. pmc EPR spectroscopic and computational characterization of the hydroxyethylidene-thiamine pyrophosphate radical intermediate of pyruvate:ferredoxin oxidoreductase
    Steven O Mansoorabadi
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53726 4087, USA
    Biochemistry 45:7122-31. 2006
  10. ncbi request reprint The ubiquity of iron
    Perry A Frey
    Department of Biochemistry, University of Wisconsin Madison, 1710 University Avenue, Madison, WI 53726, USA
    ACS Chem Biol 7:1477-81. 2012

Collaborators

  • Stephen W Ragsdale
  • Ruma Banerjee
  • Adrian D Hegeman
  • Vahe Bandarian
  • Perry A Frey
  • Steven O Mansoorabadi
  • Russell R Poyner
  • Kuo Hsiang Tang
  • N Cecilia Martinez-Gomez
  • Gunes Bender
  • Phillip A Schwartz
  • Paul A Sims
  • Diana M Downs
  • Russell Lobrutto
  • Vladimir Krymov
  • Cristina Furdui
  • W Wallace Cleland
  • Ann L Menefee
  • Gary J Gerfen
  • Tadhg P Begley
  • Olafur Th Magnusson
  • Jonathan Melnick
  • Todd M Larsen
  • Javier Seravalli
  • Mark A Anderson
  • Nisso Fazliddinova
  • Monica Vlasie
  • Rugmini Padmakumar

Detail Information

Publications15

  1. pmc Radical reactions of thiamin pyrophosphate in 2-oxoacid oxidoreductases
    George H Reed
    Department of Biochemistry, University of Wisconsin Madison, 1710 University Avenue, Madison, WI 53726, USA
    Biochim Biophys Acta 1824:1291-8. 2012
    ..This article is part of a Special Issue entitled: Radical SAM enzymes and Radical Enzymology...
  2. pmc Identification of the substrate radical intermediate derived from ethanolamine during catalysis by ethanolamine ammonia-lyase
    Gunes Bender
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53726, USA
    Biochemistry 47:11360-6. 2008
    ....
  3. pmc Analysis of the Cob(II)alamin-5'-deoxy-3',4'-anhydroadenosyl radical triplet spin system in the active site of diol dehydrase
    Steven O Mansoorabadi
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53726 4087, USA
    Biochemistry 45:14362-70. 2006
    ..Homolytic cleavage of the cobalt-carbon bond of the analogue in the absence of the substrate indicates that, in diol dehydrase, binding of the coenzyme to the protein weakens the bond prior to binding of the substrate...
  4. pmc Characterization of a succinyl-CoA radical-cob(II)alamin spin triplet intermediate in the reaction catalyzed by adenosylcobalamin-dependent methylmalonyl-CoA mutase
    Steven O Mansoorabadi
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53726 4087, USA
    Biochemistry 44:3153-8. 2005
    ..The interspin distance and the Euler angles were used to position this product radical into the active site of the enzyme...
  5. pmc Reaction of AdoMet with ThiC generates a backbone free radical
    N Cecilia Martinez-Gomez
    Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biochemistry 48:217-9. 2009
    ..Regardless of whether the backbone radical is part of the catalytic machinery, its presence provides evidence that ThiC employs free radical chemistry as expected for radical SAM enzymes...
  6. pmc Radical triplets and suicide inhibition in reactions of 4-thia-D- and 4-thia-L-lysine with lysine 5,6-aminomutase
    Kuo Hsiang Tang
    Department of Biochemistry, University of Wisconsin Madison, 1710 University Avenue, Madison, Wisconsin 53726, USA
    Biochemistry 48:8151-60. 2009
    ..The observation of the transient radical supports the hypothetical isomerization mechanism...
  7. pmc Probing interactions from solvent-exchangeable protons and monovalent cations with the 1,2-propanediol-1-yl radical intermediate in the reaction of dioldehydrase
    Phillip A Schwartz
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53726, USA
    Protein Sci 16:1157-64. 2007
    ..The spectroscopic evidence did not implicate a direct coordination of the activating cation and the substrate derived radical intermediate...
  8. ncbi request reprint Free radical mechanisms in enzymology
    Perry A Frey
    Department of Biochemistry, University of Wisconsin Madison, 1710 University Avenue, Madison, Wisconsin 53726, USA
    Chem Rev 106:3302-16. 2006
  9. pmc EPR spectroscopic and computational characterization of the hydroxyethylidene-thiamine pyrophosphate radical intermediate of pyruvate:ferredoxin oxidoreductase
    Steven O Mansoorabadi
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53726 4087, USA
    Biochemistry 45:7122-31. 2006
    ..The hyperfine splitting constants and g-values are not compatible with those predicted for a nonplanar, sigma/n-type cation radical...
  10. ncbi request reprint The ubiquity of iron
    Perry A Frey
    Department of Biochemistry, University of Wisconsin Madison, 1710 University Avenue, Madison, WI 53726, USA
    ACS Chem Biol 7:1477-81. 2012
    ..It seems likely that other solid planets, formed by the same processes as Earth, would also foster the evolution of life and that iron would be similarly important to life on those planets as it is on Earth...
  11. pmc Probing nitrogen-sensitive steps in the free-radical-mediated deamination of amino alcohols by ethanolamine ammonia-lyase
    Russell R Poyner
    Department of Biochemistry, Enzyme Institute, University of Wisconsin, 1710 University Avenue, Madison, Wisconsin 53726, USA
    J Am Chem Soc 128:7120-1. 2006
    ..15N-sensitive steps are significant contributors to V/K with (S)-2-aminopropanol...
  12. ncbi request reprint Radical mechanisms in adenosylcobalamin-dependent enzymes
    George H Reed
    Department of Biochemistry, University of Wisconsin Madison, 1710 University Avenue, Madison, Wisconsin 53726 4087, USA
    Curr Opin Chem Biol 8:477-83. 2004
    ..Analogs of substrates and of adenosylcobalamin have been used to explore the fidelity of the enzyme active sites and the radical pathways...
  13. doi request reprint Pyridoxal-5'-phosphate as the catalyst for radical isomerization in reactions of PLP-dependent aminomutases
    Perry A Frey
    Department of Biochemistry, University of Wisconsin Madison, 1710 University Avenue, Madison, WI 53726, USA
    Biochim Biophys Acta 1814:1548-57. 2011
    ..This article is part of a Special Issue entitled: Pyridoxal Phospate Enzymology...
  14. pmc Structure and catalytic properties of an engineered heterodimer of enolase composed of one active and one inactive subunit
    Paul A Sims
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI, 53726, USA
    J Mol Biol 355:422-31. 2006
    ..A comparison of enolase structures obtained from crystals belonging to different space groups suggests that asymmetric dimers can be a consequence of the asymmetric positioning of the subunits within the crystal lattice...
  15. pmc The positions of radical intermediates in the active sites of adenosylcobalamin-dependent enzymes
    George H Reed
    Department of Biochemistry, University of Wisconsin Madison, 1710 University Avenue, Madison, Wisconsin 53726, USA
    Curr Opin Struct Biol 13:716-21. 2003
    ..Interspin distances greater than 5A require motion of one or more of the participating species. EPR spectroscopy provides a convenient means to determine the structures of these transient intermediates...