Research Topics
| ROBERT RAWSONSummaryAffiliation: University of Texas Southwestern Medical Center Country: USA Publications
Research Grants
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Publications
Intriguing parasites and intramembrane proteasesRobert B Rawson
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Genes Dev 22:1561-6. 2008..EhROM1 is found at the base of the cap rather than in the cap proper, suggesting a role in receptor shedding and implying that EhROM1 is crucial for amoebal infection...
The SREBP pathway--insights from Insigs and insectsRobert B Rawson
Department of Molecular Genetics, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
Nat Rev Mol Cell Biol 4:631-40. 2003....- The SREBP pathway in Drosophila: regulation by palmitate, not sterolsAdam C Seegmiller
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Dev Cell 2:229-38. 2002..Instead, dSREBP processing is blocked by palmitic acid. These findings suggest that the ancestral SREBP pathway functions to maintain membrane integrity rather than to control cholesterol homeostasis... - Isolation of mutant cells lacking Insig-1 through selection with SR-12813, an agent that stimulates degradation of 3-hydroxy-3-methylglutaryl-coenzyme A reductaseNavdar Sever
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046, USA
J Biol Chem 279:43136-47. 2004..These results provide formal genetic proof for the essential role of Insig-1 in feedback control of lipid synthesis in cultured cells... - Reconstitution of sterol-regulated endoplasmic reticulum-to-Golgi transport of SREBP-2 in insect cells by co-expression of mammalian SCAP and InsigsIrina Y Dobrosotskaya
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046
J Biol Chem 278:35837-43. 2003..They indicate that insect cells can respond to sterols when proper regulatory proteins are expressed... - Three mutations in sterol-sensing domain of SCAP block interaction with insig and render SREBP cleavage insensitive to sterolsDaisuke Yabe
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Proc Natl Acad Sci U S A 99:16672-7. 2002..Thus, three different point mutations, each within the sterol-sensing domain of SCAP, prevent sterol-induced binding of SCAP to insig proteins and abolish feedback regulation of SREBP processing by sterols... - Mutant mammalian cells as tools to delineate the sterol regulatory element-binding protein pathway for feedback regulation of lipid synthesisJoseph L Goldstein
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046, USA
Arch Biochem Biophys 397:139-48. 2002..Their cDNAs and genes were isolated by genetic complementation or by expression cloning. The somatic cell genetic approach described in this article should prove useful for unraveling other complex biochemical pathways in animal cells... - Regulated intramembrane proteolysis: from the endoplasmic reticulum to the nucleusRobert B Rawson
Department of Molecular Genetics, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9046, USA
Essays Biochem 38:155-68. 2002.... 
Rhomboid proteases: familiar features in unfamiliar phasesBilal Amarneh
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, 75390 9046, USA
Mol Cell 36:922-3. 2009..2009) identify a sequence motif underlying cleavage site specificity for the rhomboid proteases. This sheds light on potential mechanisms by which intramembrane-cleaving proteases cleave their substrates...- Alternative processing of sterol regulatory element binding protein during larval development in Drosophila melanogasterKrista A Matthews
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046, USA
Genetics 181:119-28. 2009..Despite loss of dS2P, dSREBP is processed in mutant larvae. Therefore, larvae have an alternative cleavage mechanism for producing transcriptionally active dSREBP, and this permits survival of dS2P mutants... - An ARC light on lipid metabolismRobert B Rawson
Department of Molecular Genetics, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
Cell Metab 4:181-3. 2006..present a comprehensive series of experiments, spanning a wide range of disciplines, that identify ARC105 as a component of the ARC complex that interacts directly with SREBP and is necessary for SREBP function (Yang et al., 2006)... - Fatty acid auxotrophy in Drosophila larvae lacking SREBPAmit S Kunte
Department of Molecular Genetics, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, Texas 75390, USA
Cell Metab 3:439-48. 2006..The role, if any, of dSREBP in adults is not yet apparent. These data indicate that dSREBP deficiency renders Drosophila larvae auxotrophic for fatty acids... - Activation of sterol regulatory element-binding protein by the caspase Drice in Drosophila larvaeBilal Amarneh
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046, USA
J Biol Chem 284:9674-82. 2009..Drice-dependent activation of dSREBP explains why flies lacking dS2P are viable, and flies lacking dSREBP itself are not... - Dual roles for cholesterol in mammalian cellsFang Xu
Center for Human Nutrition, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
Proc Natl Acad Sci U S A 102:14551-6. 2005.... - Activation of sterol regulatory element binding proteins in the absence of Scap in Drosophila melanogasterKrista A Matthews
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
Genetics 185:189-98. 2010..Thus, dScap and dS2P, essential components of the SREBP activation machinery in mammalian cells, are dispensable in Drosophila owing to different compensatory mechanisms... 
COPI activity coupled with fatty acid biosynthesis is required for viral replicationSara Cherry
University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America
PLoS Pathog 2:e102. 2006..Additionally, because these pathways are also limiting in flies and in human cells infected with the related RNA virus poliovirus, they may represent novel targets for antiviral therapies...
Research Grants
- Regulation of Lipid and Membrane Synthesis in DrosophilaROBERT RAWSON; Fiscal Year: 2007..These hypotheses will be easier to test in the simpler fly model, thereby complementing rather than duplicating studies of SREBPs ongoing in other laboratories. ..
- Regulation of Lipid and Membrane Synthesis in DrosophilaROBERT RAWSON; Fiscal Year: 2009..These hypotheses will be easier to test in the simpler fly model, thereby complementing rather than duplicating studies of SREBPs ongoing in other laboratories. ..