Mariusz Ratajczak

Summary

Affiliation: University of Louisville
Country: USA

Publications

  1. doi request reprint Phenotypic and functional characterization of hematopoietic stem cells
    Mariusz Z Ratajczak
    Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky 40202, USA
    Curr Opin Hematol 15:293-300. 2008
  2. pmc Cells expressing early cardiac markers reside in the bone marrow and are mobilized into the peripheral blood after myocardial infarction
    Magda Kucia
    Stem Cell Biology Program, University of Louisville, Louisville, Ken 40202, USA
    Circ Res 95:1191-9. 2004
  3. doi request reprint The use of chemokine receptor agonists in stem cell mobilization
    Mariusz Z Ratajczak
    University of Louisville, Stem Cell Institute at James Graham Brown Cancer Center, 500 S Floyd Street, Room 107, Louisville, KY 40202, USA
    Expert Opin Biol Ther 12:287-97. 2012
  4. doi request reprint The role of sphingosine-1 phosphate and ceramide-1 phosphate in trafficking of normal stem cells and cancer cells
    Mariusz Z Ratajczak
    University of Louisville, Stem Cell Institute, James Graham Brown Cancer Center, 500 S Floyd Street, Rm 107, Louisville, KY 40202, USA 1 502 852 1788 1 502 852 3032
    Expert Opin Ther Targets 18:95-107. 2014
  5. pmc Morphology of ovaries in laron dwarf mice, with low circulating plasma levels of insulin-like growth factor-1 (IGF-1), and in bovine GH-transgenic mice, with high circulating plasma levels of IGF-1
    Sylwia Słuczanowska-Głąbowska
    Department of Physiology, Pomeranian Medical University, Powstancow Wielkopolskich 72, 70 111, Szczecin, Poland
    J Ovarian Res 5:18. 2012
  6. pmc Bioactive lipids S1P and C1P are prometastatic factors in human rhabdomyosarcoma, and their tissue levels increase in response to radio/chemotherapy
    Gabriela Schneider
    Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, 500 S Floyd Street, Louisville, KY 40202, USA
    Mol Cancer Res 11:793-807. 2013
  7. doi request reprint An emerging link in stem cell mobilization between activation of the complement cascade and the chemotactic gradient of sphingosine-1-phosphate
    Mariusz Z Ratajczak
    Stem Cell Biology Program at James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Prostaglandins Other Lipid Mediat 104:122-9. 2013
  8. pmc Innate immunity derived factors as external modulators of the CXCL12-CXCR4 axis and their role in stem cell homing and mobilization
    Mariusz Z Ratajczak
    Stem Cell Biology Program at the James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky, USA
    Theranostics 3:3-10. 2013
  9. ncbi request reprint Innate immunity as orchestrator of bone marrow homing for hematopoietic stem/progenitor cells
    Mariusz Z Ratajczak
    Stem Cell Biology Program at the James Graham Brown Cancer Center, University of Louisville, 500 S Floyd Street, Rm 107, Louisville, KY 40202, USA
    Adv Exp Med Biol 735:219-32. 2013
  10. doi request reprint CD133 Expression Strongly Correlates with the Phenotype of Very Small Embryonic-/Epiblast-Like Stem Cells
    Mariusz Z Ratajczak
    Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, 500 S Floyd Street, 40202 Rm 107, Louisville, KY, USA
    Adv Exp Med Biol 777:125-41. 2013

Research Grants

  1. The CXCR4-SDF-1 Axis in Metastatic Rhabdomyosarcoma
    Mariusz Ratajczak; Fiscal Year: 2009
  2. Novel hematopoietic effects of C3 cleavage fragments
    Mariusz Z Ratajczak; Fiscal Year: 2010
  3. Novel hematopoietic effects of C3 cleavage fragments
    Mariusz Ratajczak; Fiscal Year: 2007
  4. CELLULAR AND MOLECULAR BASIS OF HIV BASED THROMBOCYTOPEN
    Mariusz Ratajczak; Fiscal Year: 2002

Collaborators

Detail Information

Publications108 found, 100 shown here

  1. doi request reprint Phenotypic and functional characterization of hematopoietic stem cells
    Mariusz Z Ratajczak
    Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky 40202, USA
    Curr Opin Hematol 15:293-300. 2008
    ..This article summarizes recent progress in understanding the developmental hierarchy of the stem cell compartment in hematopoietic organs and discusses assays and phenotypic markers that allow their identification...
  2. pmc Cells expressing early cardiac markers reside in the bone marrow and are mobilized into the peripheral blood after myocardial infarction
    Magda Kucia
    Stem Cell Biology Program, University of Louisville, Louisville, Ken 40202, USA
    Circ Res 95:1191-9. 2004
    ..The present findings provide a novel paradigm that could reconcile current controversies and a rationale for investigating the use of BM-derived cardiac progenitors for myocardial regeneration...
  3. doi request reprint The use of chemokine receptor agonists in stem cell mobilization
    Mariusz Z Ratajczak
    University of Louisville, Stem Cell Institute at James Graham Brown Cancer Center, 500 S Floyd Street, Room 107, Louisville, KY 40202, USA
    Expert Opin Biol Ther 12:287-97. 2012
    ....
  4. doi request reprint The role of sphingosine-1 phosphate and ceramide-1 phosphate in trafficking of normal stem cells and cancer cells
    Mariusz Z Ratajczak
    University of Louisville, Stem Cell Institute, James Graham Brown Cancer Center, 500 S Floyd Street, Rm 107, Louisville, KY 40202, USA 1 502 852 1788 1 502 852 3032
    Expert Opin Ther Targets 18:95-107. 2014
    ....
  5. pmc Morphology of ovaries in laron dwarf mice, with low circulating plasma levels of insulin-like growth factor-1 (IGF-1), and in bovine GH-transgenic mice, with high circulating plasma levels of IGF-1
    Sylwia Słuczanowska-Głąbowska
    Department of Physiology, Pomeranian Medical University, Powstancow Wielkopolskich 72, 70 111, Szczecin, Poland
    J Ovarian Res 5:18. 2012
    ..abstract:..
  6. pmc Bioactive lipids S1P and C1P are prometastatic factors in human rhabdomyosarcoma, and their tissue levels increase in response to radio/chemotherapy
    Gabriela Schneider
    Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, 500 S Floyd Street, Louisville, KY 40202, USA
    Mol Cancer Res 11:793-807. 2013
    ..These data indicate that bioactive lipids play a vital role in dissemination of RMS and contribute to the unwanted side effects of radio/chemotherapy by creating a prometastatic microenvironment...
  7. doi request reprint An emerging link in stem cell mobilization between activation of the complement cascade and the chemotactic gradient of sphingosine-1-phosphate
    Mariusz Z Ratajczak
    Stem Cell Biology Program at James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Prostaglandins Other Lipid Mediat 104:122-9. 2013
    ....
  8. pmc Innate immunity derived factors as external modulators of the CXCL12-CXCR4 axis and their role in stem cell homing and mobilization
    Mariusz Z Ratajczak
    Stem Cell Biology Program at the James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky, USA
    Theranostics 3:3-10. 2013
    ....
  9. ncbi request reprint Innate immunity as orchestrator of bone marrow homing for hematopoietic stem/progenitor cells
    Mariusz Z Ratajczak
    Stem Cell Biology Program at the James Graham Brown Cancer Center, University of Louisville, 500 S Floyd Street, Rm 107, Louisville, KY 40202, USA
    Adv Exp Med Biol 735:219-32. 2013
    ..We also propose modulation of CC as a novel strategy to optimize/accelerate homing of HSPCs...
  10. doi request reprint CD133 Expression Strongly Correlates with the Phenotype of Very Small Embryonic-/Epiblast-Like Stem Cells
    Mariusz Z Ratajczak
    Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, 500 S Floyd Street, 40202 Rm 107, Louisville, KY, USA
    Adv Exp Med Biol 777:125-41. 2013
    ..However, still more labor is needed to characterize better at a molecular level these rare cells...
  11. ncbi request reprint Innate immunity as orchestrator of bone marrow homing for hematopoietic stem/progenitor cells
    Mariusz Z Ratajczak
    Stem Cell Biology Program at the James Graham Brown Cancer Center, University of Louisville, 500 S Floyd Street, Rm 107, Louisville, KY, 40202, USA
    Adv Exp Med Biol 734:219-32. 2013
    ..We also propose modulation of CC as a novel strategy to optimize/accelerate homing of HSPCs...
  12. doi request reprint Pluripotent and multipotent stem cells in adult tissues
    M Z Ratajczak
    Stem Cell Biology Program, James Graham Brown Cancer Center, University of Louisville, Kentucky, USA
    Adv Med Sci 57:1-17. 2012
    ..These different populations of stem cells will be reviewed in this paper...
  13. pmc The expanding family of bone marrow homing factors for hematopoietic stem cells: stromal derived factor 1 is not the only player in the game
    Mariusz Z Ratajczak
    Stem Cell Biology Program at the James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    ScientificWorldJournal 2012:758512. 2012
    ....
  14. ncbi request reprint Igf2-H19, an imprinted tandem gene, is an important regulator of embryonic development, a guardian of proliferation of adult pluripotent stem cells, a regulator of longevity, and a 'passkey' to cancerogenesis
    Mariusz Z Ratajczak
    Stem Cell Biology Program at the James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky 40202, USA
    Folia Histochem Cytobiol 50:171-9. 2012
    ..In this review, we will discuss the biological consequences of changes in Igf2-H19 expression...
  15. pmc Very small embryonic/epiblast-like stem cells (VSELs) and their potential role in aging and organ rejuvenation--an update and comparison to other primitive small stem cells isolated from adult tissues
    Mariusz Z Ratajczak
    Stem Cell Biology Program at the James Graham Brown Cancer Center, University of Louisville, Louisville, KT, USA
    Aging (Albany NY) 4:235-46. 2012
    ..Finally, while murine BM-derived VSELs have been extensively characterized more work is needed to better characterize these small cells at the molecular level in humans...
  16. ncbi request reprint Umbilical cord blood-derived very small embryonic like stem cells (VSELs) as a source of pluripotent stem cells for regenerative medicine
    Mariusz Z Ratajczak
    Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, 500 S Floyd Street, Rm 107 Louisville, KY 40202, USA
    Pediatr Endocrinol Rev 9:639-43. 2012
    ..We propose that UCB-VSELs may eventually be employed as a source of pluripotent stem cells in regenerative medicine...
  17. doi request reprint A novel perspective on stem cell homing and mobilization: review on bioactive lipids as potent chemoattractants and cationic peptides as underappreciated modulators of responsiveness to SDF-1 gradients
    M Z Ratajczak
    Stem Cell Institute, James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Leukemia 26:63-72. 2012
    ..Together, these phenomena support a picture in which the SDF-1-CXCR4 axis modulates homing, BM retention and mobilization of HSPCs in a more complex way than previously envisioned...
  18. ncbi request reprint The pleiotropic effects of the SDF-1-CXCR4 axis in organogenesis, regeneration and tumorigenesis
    M Z Ratajczak
    Stem Cell Biology Program at James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Leukemia 20:1915-24. 2006
    ....
  19. doi request reprint Evidence that very small embryonic-like stem cells are mobilized into peripheral blood
    Magda J Kucia
    Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, 500 South Floyd Street, Room 107, Louisville, Kentucky 40202, USA
    Stem Cells 26:2083-92. 2008
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  20. pmc Very small embryonic-like stem cells: characterization, developmental origin, and biological significance
    Mariusz Z Ratajczak
    Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Exp Hematol 36:742-51. 2008
    ..Finally, we envision that in pathological situations VSELs are involved in development of some malignancies (e.g., teratomas, germinal tumors)...
  21. doi request reprint Very small embryonic-like (VSEL) stem cells: purification from adult organs, characterization, and biological significance
    Mariusz Z Ratajczak
    Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, 500 South Floyd Street, Louisville, KY 40202, USA
    Stem Cell Rev 4:89-99. 2008
    ..We named these primitive cells very small embryonic-like (VSEL) SCs. Our data indicate that VSELs may differentiate into cells from all three germ layers...
  22. pmc Hunt for pluripotent stem cell -- regenerative medicine search for almighty cell
    Mariusz Z Ratajczak
    Stem Cell Institute, James Graham Brown Cancer Center, University of Louisville, 500 South Floyd Street, Louisville, KY 40202, USA
    J Autoimmun 30:151-62. 2008
    ..This review will summarize current approaches and limitations to isolate PSC from adult tissues or, alternatively, to generate it by nuclear reprogramming from already differentiated somatic cells...
  23. ncbi request reprint A pivotal role of activation of complement cascade (CC) in mobilization of hematopoietic stem/progenitor cells (HSPC)
    Mariusz Z Ratajczak
    James Graham Brown Cancer Center, Stem Cell Institute, University of Louisville, Louisville, KY 40202, USA
    Adv Exp Med Biol 632:47-60. 2008
    ..Hence modulation of CC activation could allow for the development of more efficient mobilization strategies in patients who are poor mobilizers of HSPC...
  24. ncbi request reprint Morphological characterization of very small embryonic-like stem cells (VSELs) by ImageStream system analysis
    Ewa K Zuba-Surma
    Stem Cell Biology Institute, University of Louisville, Louisville, KY 40202, USA
    J Cell Mol Med 12:292-303. 2008
    ..Thus, FACS-based sorting strategies should consider that adult tissues harbour small primitive cells that are larger than platelets and smaller than erythrocytes...
  25. ncbi request reprint Bone-marrow-derived stem cells--our key to longevity?
    Mariusz Z Ratajczak
    Stem Cell Institute, University of Louisville, 500 South Floyd Street, Louisville, KY 40202, USA
    J Appl Genet 48:307-19. 2007
    ....
  26. ncbi request reprint A hypothesis for an embryonic origin of pluripotent Oct-4(+) stem cells in adult bone marrow and other tissues
    M Z Ratajczak
    Stem Cell Biology Program at James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Leukemia 21:860-7. 2007
    ..The germ lineage in order to pass genes on to the next generations creates soma and thus becomes a 'mother lineage' for all somatic cell lineages present in the adult body...
  27. pmc Very small embryonic-like (VSEL) stem cells in adult organs and their potential role in rejuvenation of tissues and longevity
    Mariusz Z Ratajczak
    Stem Cell Institute at James Graham Brown Cancer Center, Hoenig Endowed Chair in Cancer Biology, University of Louisville, Louisville, KY 40202, USA
    Exp Gerontol 43:1009-17. 2008
    ..Thus, these cells are potential SCs candidates for regenerative medicine and we envision that the regenerative potential of these cells could be harnessed to decelerate the aging processes...
  28. pmc Very small embryonic/epiblast-like stem cells: a missing link to support the germ line hypothesis of cancer development?
    Mariusz Z Ratajczak
    or Magda Kucia, Ph D, Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, 500 S Floyd Street, Rm 107, Louisville, KY 40202, USA
    Am J Pathol 174:1985-92. 2009
    ....
  29. doi request reprint The role of innate immunity in trafficking of hematopoietic stem cells-an emerging link between activation of complement cascade and chemotactic gradients of bioactive sphingolipids
    Mariusz Z Ratajczak
    Stem Cell Biology Program at the James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA
    Adv Exp Med Biol 946:37-54. 2012
    ..Finally, the chemotactic activity of all chemoattractants for HSPCs including SDF-1, S1P and C1P is enhanced in presence of CC cleavage fragments (e.g., C3a) and MAC that is a final product of CC activation...
  30. pmc RasGrf1: genomic imprinting, VSELs, and aging
    Mariusz Z Ratajczak
    Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, KY 40202, USA
    Aging (Albany NY) 3:692-7. 2011
    ..All this in toto supports a concept that the sperm genome may have a detrimental effect on longevity in mammals. We will discuss a role of RasGrf1 on life span in context of genomic imprinting and VSELs...
  31. pmc Hematopoietic differentiation of umbilical cord blood-derived very small embryonic/epiblast-like stem cells
    J Ratajczak
    Stem Cell Institute at the James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Leukemia 25:1278-85. 2011
    ..Overall, our data suggest that UCB-VSELs correspond to the most primitive population of HSPCs in UCB...
  32. ncbi request reprint Stem cells for neural regeneration--a potential application of very small embryonic-like stem cells
    J Ratajczak
    Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville Kentucky 40202, USA
    J Physiol Pharmacol 62:3-12. 2011
    ..In conclusion human VSELs could be potentially harnessed in regenerative medicine as a source of stem cells for neurogenesis...
  33. pmc A novel insight into aging: are there pluripotent very small embryonic-like stem cells (VSELs) in adult tissues overtime depleted in an Igf-1-dependent manner?
    Mariusz Z Ratajczak
    Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Aging (Albany NY) 2:875-83. 2010
    ..Our preliminary data in long-living Igf-1-signaling-deficient mice show that these animals possess a 3-4 times higher number of VSELs deposited in adult BM, lending support to this novel hypothesis...
  34. doi request reprint Innate immunity as orchestrator of stem cell mobilization
    M Z Ratajczak
    Stem Cell Biology Program at the James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Leukemia 24:1667-75. 2010
    ..In this review we will present mounting evidence that innate immunity orchestrates this evolutionarily conserved mechanism of HSPC mobilization...
  35. ncbi request reprint Mechanisms of cancer metastasis: involvement of cancer stem cells?
    M Ratajczak
    Stem Cell Institute at the James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Minerva Med 101:179-91. 2010
    ..As we hypothesize, these cells could: 1) give rise to some primitive types of tumors; and 2) may have a direct role in cancer expansion by being involved in tumor angiogenesis and formation of tumor stroma...
  36. pmc Novel insight into stem cell mobilization-plasma sphingosine-1-phosphate is a major chemoattractant that directs the egress of hematopoietic stem progenitor cells from the bone marrow and its level in peripheral blood increases during mobilization due to
    M Z Ratajczak
    Stem Cell Biology Program at the James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Leukemia 24:976-85. 2010
    ..We conclude and propose a new paradigm that S1P is a crucial chemoattractant for BM-residing HSPCs and that CC through MAC induces the release of S1P from erythrocytes for optimal egress/mobilization of HSPCs...
  37. pmc Epiblast/germ line hypothesis of cancer development revisited: lesson from the presence of Oct-4+ cells in adult tissues
    Mariusz Z Ratajczak
    Stem Cell Institute, James Graham Brown Cancer Center, University of Louisville, 500 S Floyd Street, Louisville, KY 40202, USA
    Stem Cell Rev 6:307-16. 2010
    ..However, we are aware that this working hypothesis requires further direct experimental confirmation...
  38. ncbi request reprint [Physiological and pathological consequences of a presence of germ line stem cells in adult tissues]
    Mariusz Z Ratajczak
    Zakład Fizjologii Katedry Fizjopatologii Pomorskiej Akademii Medycznej, Szczecin
    Ginekol Pol 80:935-41. 2009
    ..These cells can become an alternative and more ethical source of the stem cells for therapy when compared to those isolated from the developing embryos...
  39. ncbi request reprint Modulation of the SDF-1-CXCR4 axis by the third complement component (C3)--implications for trafficking of CXCR4+ stem cells
    Marius Z Ratajczak
    Stem Cell Biology Program, James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Exp Hematol 34:986-95. 2006
    ..In this review we will focus on interactions between the SDF-1-CXCR4 axis and the C3 cleavage fragments in a model of mobilization, trafficking, and homing of hematopoietic stem/progenitor cells (HSPC)...
  40. ncbi request reprint Transplantation studies in C3-deficient animals reveal a novel role of the third complement component (C3) in engraftment of bone marrow cells
    M Z Ratajczak
    Stem Cell Biology Program at the James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Leukemia 18:1482-90. 2004
    ..In conclusion, a novel mechanism for HSC engraftment was identified, which involves complement activation and specific C3 fragments that promote conditioning for transplantation and enhance HSPC engraftment...
  41. ncbi request reprint Heterogeneous populations of bone marrow stem cells--are we spotting on the same cells from the different angles?
    Mariusz Z Ratajczak
    European Stem Cell Therapeutic Excellence Center, Medical College, Jagiellonian University, Cracow, Poland
    Folia Histochem Cytobiol 42:139-46. 2004
    ....
  42. ncbi request reprint Expression of functional CXCR4 by muscle satellite cells and secretion of SDF-1 by muscle-derived fibroblasts is associated with the presence of both muscle progenitors in bone marrow and hematopoietic stem/progenitor cells in muscles
    Mariusz Z Ratajczak
    Stem Cell Biology Program at James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky 40202, USA
    Stem Cells 21:363-71. 2003
    ....
  43. ncbi request reprint Cancer stem cells--normal stem cells "Jedi" that went over to the "dark side"
    Mariusz Z Ratajczak
    Stem Cell Biology Program, James Graham Brown Cancer Center, University of Louisville, KY 40202, USA
    Folia Histochem Cytobiol 43:175-81. 2005
    ..Therefore, looking both at the origin and behavioral aspects we can envision cancer SC being normal SC "Jedi" that went over to the "dark side"...
  44. ncbi request reprint Membrane-derived microvesicles: important and underappreciated mediators of cell-to-cell communication
    J Ratajczak
    The Stem Cell Biology Program, James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Leukemia 20:1487-95. 2006
    ....
  45. ncbi request reprint Quiescent CD34+ early erythroid progenitors are resistant to several erythropoietic 'inhibitory' cytokines; role of FLIP
    Janina Ratajczak
    Stem Cell Biology Program at James Graham Brown Cancer Center, University of Louisville, KY 40202, USA
    Br J Haematol 123:160-9. 2003
    ..Thus, we hypothesize that cycling cells may become more sensitive to proapoptotic stimuli (e.g. chemotherapy, inhibitory cytokines) than quiescent ones because of the downregulation of protective FLIP...
  46. ncbi request reprint Mobilization studies in mice deficient in either C3 or C3a receptor (C3aR) reveal a novel role for complement in retention of hematopoietic stem/progenitor cells in bone marrow
    Janina Ratajczak
    Stem Cell Biology Program, James Graham Brown Cancer Center, University of Louisville, 529 South Jackson St, KY 40202, USA
    Blood 103:2071-8. 2004
    ..The C3a-C3aR axis may prevent an uncontrolled release of HSPCs into peripheral blood. These data further suggest that the C3aR antagonist SB 290157 could be developed as a drug to mobilize HSPCs for transplantation...
  47. pmc Bone marrow-derived pluripotent very small embryonic-like stem cells (VSELs) are mobilized after acute myocardial infarction
    Ewa K Zuba-Surma
    Stem Cell Institute, University of Louisville, Louisville, KY 40202, USA
    J Mol Cell Cardiol 44:865-73. 2008
    ..This is the first demonstration that Oct-4+ pluripotent stem cells (VSELs) are mobilized from the BM into the PB after acute MI. This phenomenon may have pathophysiological and therapeutic implications for repair of infarcted myocardium...
  48. ncbi request reprint Adult marrow-derived very small embryonic-like stem cells and tissue engineering
    Magda Kucia
    University of Louisville, Stem Cell Institute at James Graham Brown Cancer Center, 500 South Floyd Street, Louisville, KY 40202, USA
    Expert Opin Biol Ther 7:1499-514. 2007
    ..The authors envision that VSEL-SC are epiblast-derived pluripotent stem cells and could potentially become a less-controversial source of stem cells for regeneration...
  49. ncbi request reprint Functional receptor for C3a anaphylatoxin is expressed by normal hematopoietic stem/progenitor cells, and C3a enhances their homing-related responses to SDF-1
    Ryan Reca
    Stem Cell Biology Program, University of Louisville, Louisville, KY 40202, USA
    Blood 101:3784-93. 2003
    ..C3a is the first positive regulator of this axis to be identified...
  50. doi request reprint A multi-instrumental approach to identify and purify very small embryonic like stem cells (VSELs) from adult tissues
    Mariusz Z Ratajczak
    University of Louisville, 500 South Floyd Street, Louisville, KY 40202, USA
    Micron 40:386-93. 2009
    ..We believe that VSELs could be harnessed as a source of pluripotent stem cells for regenerative medicine...
  51. doi request reprint Fetal liver very small embryonic/epiblast like stem cells follow developmental migratory pathway of hematopoietic stem cells
    Ewa K Zuba-Surma
    Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky 40202, USA
    Ann N Y Acad Sci 1176:205-18. 2009
    ..Thus, our data support the hypothesis that VSELs are a mobile pool of primitive stem cells that respond to similar chemotactic gradients as HSCs and follow their developmental migratory route...
  52. ncbi request reprint The migration of bone marrow-derived non-hematopoietic tissue-committed stem cells is regulated in an SDF-1-, HGF-, and LIF-dependent manner
    Magda Kucia
    Stem Cell Biology Program at the James Graham Brown Cancer Center, University of Louisville, 529 South Jackson Street, Louisville, KY 40202, USA
    Arch Immunol Ther Exp (Warsz) 54:121-35. 2006
    ..In this study we evaluated whether TCSCs respond to other motomorphogens, such as hepatocyte growth factor (HGF) and leukemia inhibitory factor (LIF)...
  53. ncbi request reprint Trafficking of normal stem cells and metastasis of cancer stem cells involve similar mechanisms: pivotal role of the SDF-1-CXCR4 axis
    Magda Kucia
    Stem Cell Biology Program, James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky 40202, USA
    Stem Cells 23:879-94. 2005
    ....
  54. pmc Bone marrow transplantation temporarily improves pancreatic function in streptozotocin-induced diabetes: potential involvement of very small embryonic-like cells
    Yiming Huang
    Institute for Cellular Therapeutics, James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA
    Transplantation 89:677-85. 2010
    ..The role of bone marrow (BM)-derived cells in pancreatic beta-cell regeneration remains unresolved. We examined whether BM-derived cells are recruited to the site of moderate pancreatic injury and contribute to beta-cell regeneration...
  55. doi request reprint Mobilization of hematopoietic progenitor cells by yeast-derived beta-glucan requires activation of matrix metalloproteinase-9
    Daniel E Cramer
    Tumor Immunobiology Program, Department of Medicine, James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky 40202, USA
    Stem Cells 26:1231-40. 2008
    ..Thus, PGG beta-glucan is an agent that enhances HPC mobilization and may improve the outcome of clinical stem cell transplantation...
  56. doi request reprint Optimization of isolation and further characterization of umbilical-cord-blood-derived very small embryonic/ epiblast-like stem cells (VSELs)
    Ewa K Zuba-Surma
    Stem Cell Biology Institute, University of Louisville, KY 40202, USA
    Eur J Haematol 84:34-46. 2010
    ..The whole isolation procedure takes approximately 2-3 h per UCB unit and isolated cells are highly enriched for an Oct-4(+) and SSEA-4(+) population of small Lin(-)/CD45(-)/CD133(+) cells...
  57. ncbi request reprint Flt3-ligand-mobilized peripheral blood, but not Flt3-ligand-expanded bone marrow, facilitating cells promote establishment of chimerism and tolerance
    Yiming Huang
    Institute for Cellular Therapeutics, University of Louisville, 570 S Preston Street, Suite 404, Louisville, Kentucky 40202 1760, USA
    Stem Cells 24:936-48. 2006
    ..These findings may have implications in development of strategies for retaining function of ex vivo manipulated FCs and HSCs...
  58. doi request reprint Innate immunity: a key player in the mobilization of hematopoietic stem/progenitor cells
    HakMo Lee
    James Graham Brown Cancer Center, Stem Cell Institute, University of Louisville, 500 Floyd St, Louisville, KY 40202, USA
    Arch Immunol Ther Exp (Warsz) 57:269-78. 2009
    ....
  59. doi request reprint Clinical evidence that very small embryonic-like stem cells are mobilized into peripheral blood in patients after stroke
    Edyta Paczkowska
    Department of Physiopathology, Pomeranian Medical University, Szczecin, Poland
    Stroke 40:1237-44. 2009
    ..This raised the question of whether a similar population of cells is mobilized in human stroke patients...
  60. ncbi request reprint Tissue-specific muscle, neural and liver stem/progenitor cells reside in the bone marrow, respond to an SDF-1 gradient and are mobilized into peripheral blood during stress and tissue injury
    Magda Kucia
    Stem Cell Biology Program at James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202 3270, USA
    Blood Cells Mol Dis 32:52-7. 2004
    ....
  61. ncbi request reprint A novel role of complement in mobilization: immunodeficient mice are poor granulocyte-colony stimulating factor mobilizers because they lack complement-activating immunoglobulins
    Ryan Reca
    Stem Cell Institute, James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky 40202, USA
    Stem Cells 25:3093-100. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  62. doi request reprint Fms-related tyrosine kinase 3 expression discriminates hematopoietic stem cells subpopulations with differing engraftment-potential: identifying the most potent combination
    Yiming Huang
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
    Transplantation 85:1175-84. 2008
    ..FL significantly mobilized c-Kit(hi) and c-Kit(lo) Sca-1(+)Lin(-) cells into peripheral blood (PB). Here, we evaluated the influence of Flt3 expression on long-term repopulating ability of HSC subpopulations...
  63. ncbi request reprint Incorporation of CXCR4 into membrane lipid rafts primes homing-related responses of hematopoietic stem/progenitor cells to an SDF-1 gradient
    Marcin Wysoczynski
    Stem Cell Biology Program, James Graham Brown Cancer Center, University of Louisville, KY 40202, USA
    Blood 105:40-8. 2005
    ....
  64. ncbi request reprint The role of third complement component (C3) in homing of hematopoietic stem/progenitor cells into bone marrow
    Ryan Reca
    Stem Cell Biology Program, University of Louisville, Baxter II, STE 122, 580 S Preston Street, Louisville, KY 40202, USA
    Adv Exp Med Biol 586:35-51. 2006
  65. ncbi request reprint Sca-1 expression is associated with decreased cardiomyogenic differentiation potential of skeletal muscle-derived adult primitive cells
    Ewa K Zuba-Surma
    Institute of Molecular Cardiology, University of Louisville, Louisville, KY 40292, USA
    J Mol Cell Cardiol 41:650-60. 2006
    ....
  66. ncbi request reprint Bone marrow as a source of circulating CXCR4+ tissue-committed stem cells
    Magda Kucia
    Stem Cell Biology Program at James Graham Brown Cancer Center, University of Louisville, KY 40202, USA
    Biol Cell 97:133-46. 2005
    ....
  67. pmc "Small stem cells" in adult tissues: very small embryonic-like stem cells stand up!
    Ewa K Zuba-Surma
    Stem Cell Biology Institute, University of Louisville, Louisville, Kentucky 40202, USA
    Cytometry A 75:4-13. 2009
    ..We will also present different populations of very "small SCs" that have been recently described in the literature (e.g., spore-like cells and Lin(-)/ALDH(high) long-term repopulating hematopoietic SCs)...
  68. ncbi request reprint TNF-alpha is critical to facilitate hemopoietic stem cell engraftment and function
    Francine Rezzoug
    Institute for Cellular Therapeutics, University of Louisville, Louisville, KY 40202 1760, USA
    J Immunol 180:49-57. 2008
    ..These data demonstrate a critical role for TNF-alpha in mediating FC function. FC may have a significant impact upon the safe use of chimerism to establish tolerance to transplanted organs and tissue...
  69. ncbi request reprint CXCR4-SDF-1 signaling is active in rhabdomyosarcoma cells and regulates locomotion, chemotaxis, and adhesion
    Jolanta Libura
    Stem Cell Biology Program at the James Graham Brown Cancer Center, University of Louisville, KY 40202, USA
    Blood 100:2597-606. 2002
    ....
  70. doi request reprint CXCR7: a new SDF-1-binding receptor in contrast to normal CD34(+) progenitors is functional and is expressed at higher level in human malignant hematopoietic cells
    Maciej Tarnowski
    Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Eur J Haematol 85:472-83. 2010
    ....
  71. doi request reprint Identification of small Sca-1(+), Lin(-), CD45(-) multipotential cells in the neonatal murine retina
    Yongqing Liu
    Department of Ophthalmology and Visual Sciences, University of Louisville Health Sciences Center, Louisville, KY, USA
    Exp Hematol 37:1096-107, 1107.e1. 2009
    ..The differentiation potential of some adult stem cells is organ-restricted, but other populations appear to retain multipotential capacity...
  72. ncbi request reprint Platelet-derived microparticles stimulate proliferation, survival, adhesion, and chemotaxis of hematopoietic cells
    Monika Baj-Krzyworzeka
    Stem Cell Biology Program, James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Exp Hematol 30:450-9. 2002
    ..Peripheral blood platelet-derived microparticles (PMPs) circulate in blood and may interact directly with target cells affecting their various biological functions...
  73. pmc Very small embryonic-like stem cells are present in adult murine organs: ImageStream-based morphological analysis and distribution studies
    Ewa K Zuba-Surma
    Stem Cell Biology Institute, University of Louisville, Louisville, Kentucky 40202, USA
    Cytometry A 73:1116-27. 2008
    ..Further studies are needed to determine whether these cells, after being isolated from various adult human organs similarly to their murine BM-derived counterparts, are endowed with pluripotent stem cell properties...
  74. ncbi request reprint CXCR4-SDF-1 signalling, locomotion, chemotaxis and adhesion
    Magda Kucia
    Stem Cell Biology Program, James Graham Brown Cancer Center, University of Louisville, KY 40202, USA
    J Mol Histol 35:233-45. 2004
    ..In this review, we focus on a role of the SDF-1-CXCR4 axis in regulating the metastatic behaviour of tumour cells and discuss the molecular mechanisms that are essential to this process...
  75. ncbi request reprint Thrombopoietin, but not cytokines binding to gp130 protein-coupled receptors, activates MAPKp42/44, AKT, and STAT proteins in normal human CD34+ cells, megakaryocytes, and platelets
    Marcin Majka
    Stem Cell Biology Program at James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Exp Hematol 30:751-60. 2002
    ..We attempted to identify signal transduction pathways activated by these factors in normal human megakaryocytes...
  76. pmc Transplantation of bone marrow-derived very small embryonic-like stem cells attenuates left ventricular dysfunction and remodeling after myocardial infarction
    Buddhadeb Dawn
    Institute of Molecular Cardiology, University of Louisville, Louisville, Kentucky 40292, USA
    Stem Cells 26:1646-55. 2008
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  77. ncbi request reprint The ImageStream System: a key step to a new era in imaging
    Ewa K Zuba-Surma
    Stem Cell Biology Program, University of Louisville, Louisville, KY 40202, USA
    Folia Histochem Cytobiol 45:279-90. 2007
    ..While this article summarizes basic information about the features of ImageStream and its applications based on the available literature and it also describes our own experience...
  78. ncbi request reprint The developmental deposition of epiblast/germ cell-line derived cells in various organs as a hypothetical explanation of stem cell plasticity?
    Magda Kucia
    Stem Cell Biology Program at James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Acta Neurobiol Exp (Wars) 66:331-41. 2006
    ..In pathological situations, however they may undergo malignant transformation and give rise to tumors...
  79. ncbi request reprint SDF-1 alone and in co-operation with HGF regulates biology of human cervical carcinoma cells
    Marcin Majka
    Department of Transplantation, Polish American Institute of Pediatrics, Jagiellonian University Medical College, Cracow, Poland
    Folia Histochem Cytobiol 44:155-64. 2006
    ..Thus, our data show that the SDF-1-CXCR4 axis affects biology of HCC cells. Furthermore, we postulate that this axis might become a potential target to prevent progression of cervical cancer...
  80. doi request reprint Erythrocyte-derived microvesicles may transfer phosphatidylserine to the surface of nucleated cells and falsely 'mark' them as apoptotic
    Rui Liu
    Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Eur J Haematol 83:220-9. 2009
    ..This observation should be considered whenever Ann-V binding viability assays are employed to evaluate the quality of NCs depleted from erythrocytes via hypotonic lysis...
  81. ncbi request reprint Circulating CXCR4-positive stem/progenitor cells compete for SDF-1-positive niches in bone marrow, muscle and neural tissues: an alternative hypothesis to stem cell plasticity
    Anna Pituch-Noworolska
    Polish American Children s Hospital, Medical College, Jagiellonian University, Cracow, Poland
    Folia Histochem Cytobiol 41:13-21. 2003
    ....
  82. pmc Adult murine bone marrow-derived very small embryonic-like stem cells differentiate into the hematopoietic lineage after coculture over OP9 stromal cells
    Janina Ratajczak
    Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, 500 S Floyd Street, Louisville, KY 40202, USA
    Exp Hematol 39:225-37. 2011
    ..g., Oct-4 and SSEA-4) that we named "very small embryonic-like stem cells" (VSELs). In this report, we test the hypothesis that VSELs can differentiate along the hemato/lymphopoietic lineage...
  83. ncbi request reprint Biological role of the CXCR4-SDF-1 axis in normal human hematopoietic cells
    Marcin Majka
    James Graham Brown Cancer Center, University of Louisville, KY, USA
    Methods Mol Biol 332:103-14. 2006
    ..These cells can be used routinely for studying the role of the CXCR4-SDF-1 axis in normal human hematopoiesis...
  84. ncbi request reprint Both hepatocyte growth factor (HGF) and stromal-derived factor-1 regulate the metastatic behavior of human rhabdomyosarcoma cells, but only HGF enhances their resistance to radiochemotherapy
    Kacper Jankowski
    Stem Cell Biology Program, James Graham Brown Cancer Center, University of Louisville, 529 South Jackson Street, Louisville, KY 40202, USA
    Cancer Res 63:7926-35. 2003
    ..Signaling from the c-MET receptor may also contribute to the resistance of RMS cells to conventional treatment modalities...
  85. doi request reprint Selective upregulation of interleukin-8 by human rhabdomyosarcomas in response to hypoxia: therapeutic implications
    Marcin Wysoczynski
    James Graham Brown Cancer Center, University of Louisville, KY 40202, USA
    Int J Cancer 126:371-81. 2010
    ..We conclude that IL-8 is a pivotal proangiogenic factor released by human RMS cells in hypoxic conditions and that the targeting of IL-8 may prove to be a novel and efficient strategy for inhibiting RMS growth...
  86. pmc Beta-glucan enhances complement-mediated hematopoietic recovery after bone marrow injury
    Daniel E Cramer
    Tumor Immunobiology Program, James Graham Brown Cancer Center, University of Louisville, 580 South Preston St, Louisville, KY 40202, USA
    Blood 107:835-40. 2006
    ..Taken together, these observations suggest a novel role for C, CR3, and beta-glucan in the restoration of hematopoiesis following injury...
  87. ncbi request reprint Are bone marrow stem cells plastic or heterogenous--that is the question
    Magda Kucia
    Stem Cell Biology Program at James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Exp Hematol 33:613-23. 2005
    ..In this review, we will discuss this alternative explanation of plasticity of HSC, providing data from others and our laboratory that supports the concept that BM-derived stem cells are heterogeneous...
  88. pmc Mouse fibroblasts lacking RB1 function form spheres and undergo reprogramming to a cancer stem cell phenotype
    Yongqing Liu
    Molecular Targets Program, Brown Cancer Center, University of Louisville Health Sciences Center, Louisville, KY 40202, USA
    Cell Stem Cell 4:336-47. 2009
    ....
  89. doi request reprint Overview of very small embryonic-like stem cells (VSELs) and methodology of their identification and isolation by flow cytometric methods
    Ewa K Zuba-Surma
    Stem Cell Biology Institute, University of Louisville, Louisville, Kentucky
    Curr Protoc Cytom . 2010
    ..In the last part of this unit, we also discuss a novel flow cytometric tool, ImageStream, an imaging flow cytometer, which allows better identification and morphological analysis of sorted cells...
  90. ncbi request reprint Leukemia inhibitory factor: a newly identified metastatic factor in rhabdomyosarcomas
    Marcin Wysoczynski
    James Graham Brown Cancer Center, University of Louisville, 580 South Preston Street, Louisville, KY 40202, USA
    Cancer Res 67:2131-40. 2007
    ..Further, because we showed that the in vivo metastasis of RH30 cells is inhibited by small interfering RNA against LIF-R, molecular targeting of this axis could become a new strategy to control the metastasis of rhabdomyosarcoma...
  91. pmc Overlapping and distinct role of CXCR7-SDF-1/ITAC and CXCR4-SDF-1 axes in regulating metastatic behavior of human rhabdomyosarcomas
    Katarzyna Grymula
    Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Int J Cancer 127:2554-68. 2010
    ....
  92. pmc Detection of protein tyrosine-kinase (PTK) gene expression pattern in normal and malignant T lymphocytes by combined PTK-specific polymerase chain reaction and parallel denaturing gradient gel electrophoresis
    Zhi Yong Wang
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Mol Diagn 5:113-20. 2003
    ....
  93. ncbi request reprint Enhancing effect of platelet-derived microvesicles on the invasive potential of breast cancer cells
    Anna Janowska-Wieczorek
    Department of Medicine, University of Alberta, Edmonton, Alberta, Canada
    Transfusion 46:1199-209. 2006
    ..In this work, it was hypothesized that PMVs interact with breast cancer cells, increasing their invasiveness...
  94. ncbi request reprint Evidence that platelet-derived microvesicles may transfer platelet-specific immunoreactive antigens to the surface of endothelial cells and CD34+ hematopoietic stem/ progenitor cells--implication for the pathogenesis of immune thrombocytopenias
    Marcin Majka
    Dept of Transplantation, Polish American Institute of Pediatrics, Wielicka 265, 30 663 Krak6w, Poland
    Folia Histochem Cytobiol 45:27-32. 2007
    ..g. damage of endothelium, cytopenias)...
  95. ncbi request reprint CCR5-binding chemokines modulate CXCL12 (SDF-1)-induced responses of progenitor B cells in human bone marrow through heterologous desensitization of the CXCR4 chemokine receptor
    Marek Honczarenko
    Joint Program in Transfusion Medicine, Harvard Medical School, Children s Hospital Boston, MA, USA
    Blood 100:2321-9. 2002
    ..Thus, by modulation of CXCR4 function, signaling through CCR5 may influence B lymphopoiesis by affecting the migration and maturation of B-cell progenitors in the bone marrow microenvironment...
  96. ncbi request reprint The third complement component as modulator of platelet production
    Marcin Wysoczynski
    James Graham Brown Cancer Center University of Louisville, Stem Cell Biology Program, Louisville, KY 40202, USA
    Adv Exp Med Biol 598:226-39. 2007
  97. ncbi request reprint Hematopoietic stem cells from NOD mice exhibit autonomous behavior and a competitive advantage in allogeneic recipients
    Paula M Chilton
    Institute for Cellular Therapeutics, University of Louisville, 570 South Preston St, Suite 404, Louisville, KY 40202 1760, USA
    Blood 105:2189-97. 2005
    ..These findings may have important implications to understand the evolution of autoimmune disease and impact on potential strategies for cure...
  98. ncbi request reprint Mobilization of CD34/CXCR4+, CD34/CD117+, c-met+ stem cells, and mononuclear cells expressing early cardiac, muscle, and endothelial markers into peripheral blood in patients with acute myocardial infarction
    Wojciech Wojakowski
    Third Division of Cardiology, Silesian School of Medicine, 45 47 Ziołowa St, 40 635 Katowice, Poland
    Circulation 110:3213-20. 2004
    ....
  99. ncbi request reprint Microvesicles derived from activated platelets induce metastasis and angiogenesis in lung cancer
    Anna Janowska-Wieczorek
    Department of Medicine and Oncology, University of Alberta, Edmonton, Canada
    Int J Cancer 113:752-60. 2005
    ..Based on these findings, we suggest that PMV play an important role in tumor progression/metastasis and angiogenesis in lung cancer...
  100. ncbi request reprint Mobilization of CD34(+), CD117(+), CXCR4(+), c-met(+) stem cells is correlated with left ventricular ejection fraction and plasma NT-proBNP levels in patients with acute myocardial infarction
    Wojciech Wojakowski
    3rd Division of Cardiology, Silesian School of Medicine, 45 47 Ziołowa Street, 40 635 Katowice, Poland
    Eur Heart J 27:283-9. 2006
    ....
  101. ncbi request reprint Tumour-derived microvesicles carry several surface determinants and mRNA of tumour cells and transfer some of these determinants to monocytes
    Monika Baj-Krzyworzeka
    Department of Clinical Immunology, Polish American Institute of Paediatrics, Jagiellonian University Medical College, Wielicka 265 Str, 30 663 Cracow, Poland
    Cancer Immunol Immunother 55:808-18. 2006
    ..This implicates the novel mechanism by which tumour infiltrating macrophages may be affected by tumour cells not only by a direct cell to cell contact, soluble factors but also by TMV...

Research Grants12

  1. The CXCR4-SDF-1 Axis in Metastatic Rhabdomyosarcoma
    Mariusz Ratajczak; Fiscal Year: 2009
    ..This work will be of relevance for designing strategies to block the SDF-1-CXCR4 axis to treat CXCR4-positive cancers. ..
  2. Novel hematopoietic effects of C3 cleavage fragments
    Mariusz Z Ratajczak; Fiscal Year: 2010
    ..We will test the hypothesis that this defect is related to the lack of naturally occurring C cascade-activating IgM antibodies. ..
  3. Novel hematopoietic effects of C3 cleavage fragments
    Mariusz Ratajczak; Fiscal Year: 2007
    ..We will test the hypothesis that this defect is related to the lack of naturally occurring C cascade-activating IgM antibodies. ..
  4. CELLULAR AND MOLECULAR BASIS OF HIV BASED THROMBOCYTOPEN
    Mariusz Ratajczak; Fiscal Year: 2002
    ..In toto, the studies proposed in this grant will increase the knowledge about pathogenesis of AIDS associated thrombocytopenia and may lead to development of new strategies for its treatment of prevention. (End of Abstract) ..