M Z Ratajczak


Affiliation: University of Louisville
Country: USA


  1. Sellers Z, Bolkun L, Kloczko J, Wojtaszewska M, Lewandowski K, Moniuszko M, et al. Increased methylation upstream of the MEG3 promotor is observed in acute myeloid leukemia patients with better overall survival. Clin Epigenetics. 2019;11:50 pubmed publisher
    ..These data provide a foundation for future studies into the role of higher-order chromatin structure at DLK1-MEG3 in AML. ..
  2. Ratajczak M, Adamiak M, Thapa A, Bujko K, Brzeźniakiewicz Janus K, Lenkiewicz A. NLRP3 inflammasome couples purinergic signaling with activation of the complement cascade for the optimal release of cells from bone marrow. Leukemia. 2019;33:815-825 pubmed publisher
    ..These data are important to optimize the pharmacological mobilization of HSPCs. ..
  3. Ratajczak M, Bujko K, Mack A, Kucia M, Ratajczak J. Cancer from the perspective of stem cells and misappropriated tissue regeneration mechanisms. Leukemia. 2018;32:2519-2526 pubmed publisher
  4. Abdelbaset Ismail A, Cymer M, Borkowska Rzeszotek S, Brzeźniakiewicz Janus K, Rameshwar P, Kakar S, et al. Bioactive Phospholipids Enhance Migration and Adhesion of Human Leukemic Cells by Inhibiting Heme Oxygenase 1 (HO-1) and Inducible Nitric Oxygenase Synthase (iNOS) in a p38 MAPK-Dependent Manner. Stem Cell Rev. 2019;15:139-154 pubmed publisher
  5. Ratajczak M, Marycz K, Poniewierska Baran A, Fiedorowicz K, Zbucka Kretowska M, Moniuszko M. Very small embryonic-like stem cells as a novel developmental concept and the hierarchy of the stem cell compartment. Adv Med Sci. 2014;59:273-80 pubmed publisher
  6. Sellers Z, Schneider G, Maj M, Ratajczak M. Analysis of the Paternally-Imprinted DLK1-MEG3 and IGF2-H19 Tandem Gene Loci in NT2 Embryonal Carcinoma Cells Identifies DLK1 as a Potential Therapeutic Target. Stem Cell Rev. 2018;14:823-836 pubmed publisher
    ..These results suggest that the DLK1-MEG3 locus, and not the IGF2-H19 locus, drives the tumorigenicity of NT2 cells. Based on these results, we identified DLK1 as a novel treatment target for EC that could be downregulated by 5-azaD. ..
  7. Adamiak M, Bujko K, Cymer M, Plonka M, Glaser T, Kucia M, et al. Novel evidence that extracellular nucleotides and purinergic signaling induce innate immunity-mediated mobilization of hematopoietic stem/progenitor cells. Leukemia. 2018;32:1920-1931 pubmed publisher
    ..Thus, administration of ATP together with G-CSF or AMD3100 or inhibition of CD73 by small molecule antagonists may provide the basis for more efficient mobilization strategies. ..
  8. Ratajczak M, Adamiak M, Plonka M, Abdel Latif A, Ratajczak J. Mobilization of hematopoietic stem cells as a result of innate immunity-mediated sterile inflammation in the bone marrow microenvironment-the involvement of extracellular nucleotides and purinergic signaling. Leukemia. 2018;32:1116-1123 pubmed publisher
    ..In particular, a new finding by our laboratory is that, while extracellular ATP promotes mobilization of HSPCs, its derivative, adenosine, has the opposite (inhibitory) effect. ..
  9. Ratajczak M, Zuba Surma E, Machalinski B, Ratajczak J, Kucia M. Very small embryonic-like (VSEL) stem cells: purification from adult organs, characterization, and biological significance. Stem Cell Rev. 2008;4:89-99 pubmed publisher
    ..We named these primitive cells very small embryonic-like (VSEL) SCs. Our data indicate that VSELs may differentiate into cells from all three germ layers. ..

More Information


  1. Ratajczak M, Suszynska M. Emerging Strategies to Enhance Homing and Engraftment of Hematopoietic Stem Cells. Stem Cell Rev. 2016;12:121-8 pubmed publisher
  2. Kucia M, Wysoczynski M, Wu W, Zuba Surma E, Ratajczak J, Ratajczak M. Evidence that very small embryonic-like stem cells are mobilized into peripheral blood. Stem Cells. 2008;26:2083-92 pubmed publisher
    ..Disclosure of potential conflicts of interest is found at the end of this article. ..
  3. Schneider G, Glaser T, Lameu C, Abdelbaset Ismail A, Sellers Z, Moniuszko M, et al. Extracellular nucleotides as novel, underappreciated pro-metastatic factors that stimulate purinergic signaling in human lung cancer cells. Mol Cancer. 2015;14:201 pubmed publisher
  4. Ratajczak M, Kim C, Wojakowski W, Janowska Wieczorek A, Kucia M, Ratajczak J. Innate immunity as orchestrator of stem cell mobilization. Leukemia. 2010;24:1667-75 pubmed publisher
    ..In this review we will present mounting evidence that innate immunity orchestrates this evolutionarily conserved mechanism of HSPC mobilization. ..
  5. Gunjal P, Schneider G, Ismail A, Kakar S, Kucia M, Ratajczak M. Evidence for induction of a tumor metastasis-receptive microenvironment for ovarian cancer cells in bone marrow and other organs as an unwanted and underestimated side effect of chemotherapy/radiotherapy. J Ovarian Res. 2015;8:20 pubmed publisher
  6. Ratajczak M, Ratajczak J. Horizontal transfer of RNA and proteins between cells by extracellular microvesicles: 14 years later. Clin Transl Med. 2016;5:7 pubmed publisher
    ..In this review we will summarize the impressive progress in this field-14 years after initial report. ..
  7. Ratajczak M, Ciechanowicz A, Kucharska Mazur J, Samochowiec J. Stem cells and their potential clinical applications in psychiatric disorders. Prog Neuropsychopharmacol Biol Psychiatry. 2018;80:3-9 pubmed publisher
    ..However, the main question remains: Will it be possible in the future to modulate the stem cell compartment to reverse psychiatric problems? ..
  8. Ratajczak M, Ratajczak J, Suszynska M, Miller D, Kucia M, Shin D. A Novel View of the Adult Stem Cell Compartment From the Perspective of a Quiescent Population of Very Small Embryonic-Like Stem Cells. Circ Res. 2017;120:166-178 pubmed publisher
  9. Adamiak M, Abdelbaset Ismail A, Moore J, Zhao J, Abdel Latif A, Wysoczynski M, et al. Inducible Nitric Oxide Synthase (iNOS) Is a Novel Negative Regulator of Hematopoietic Stem/Progenitor Cell Trafficking. Stem Cell Rev. 2017;13:92-103 pubmed publisher
    ..Since HO-1 is a negative regulator of cell migration, the inhibitory effects of iNOS identified by us can be at least partially explained by its enhancing the HO-1 level in BM cells. ..
  10. Ratajczak M, Shin D, Ratajczak J, Kucia M, Bartke A. A novel insight into aging: are there pluripotent very small embryonic-like stem cells (VSELs) in adult tissues overtime depleted in an Igf-1-dependent manner?. Aging (Albany NY). 2010;2:875-83 pubmed
    ..Our preliminary data in long-living Igf-1-signaling-deficient mice show that these animals possess a 3-4 times higher number of VSELs deposited in adult BM, lending support to this novel hypothesis. ..
  11. Ratajczak M, Kucia M, Liu R, Shin D, Bryndza E, Masternak M, et al. RasGrf1: genomic imprinting, VSELs, and aging. Aging (Albany NY). 2011;3:692-7 pubmed
    ..All this in toto supports a concept that the sperm genome may have a detrimental effect on longevity in mammals. We will discuss a role of RasGrf1 on life span in context of genomic imprinting and VSELs. ..
  12. Abdelbaset Ismail A, Pędziwiatr D, Suszyńska E, Sluczanowska Glabowska S, Schneider G, Kakar S, et al. Vitamin D3 stimulates embryonic stem cells but inhibits migration and growth of ovarian cancer and teratocarcinoma cell lines. J Ovarian Res. 2016;9:26 pubmed publisher
    ..We postulate that while Vitamin D3 as anticancer drug inhibits proliferation of malignant cells, it may protect normal stem cells that play an important role in development and tissue/organ regeneration. ..
  13. Ratajczak M, Suszynska M, Kucia M. Does it make sense to target one tumor cell chemotactic factor or its receptor when several chemotactic axes are involved in metastasis of the same cancer?. Clin Transl Med. 2016;5:28 pubmed publisher
  14. Ratajczak M, Bartke A, Darzynkiewicz Z. Prolonged Growth Hormone/Insulin/Insulin-like Growth Factor Nutrient Response Signaling Pathway as a Silent Killer of Stem Cells and a Culprit in Aging. Stem Cell Rev. 2017;13:443-453 pubmed publisher
    ..In this context, we can envision nutrient response GH/INS/IGF signaling pathway as a lethal factor for these most primitive stem cells and an important culprit in aging. ..
  15. Ratajczak M, Shin D, Liu R, Mierzejewska K, Ratajczak J, Kucia M, et al. Very small embryonic/epiblast-like stem cells (VSELs) and their potential role in aging and organ rejuvenation--an update and comparison to other primitive small stem cells isolated from adult tissues. Aging (Albany NY). 2012;4:235-46 pubmed
    ..Finally, while murine BM-derived VSELs have been extensively characterized more work is needed to better characterize these small cells at the molecular level in humans. ..
  16. request reprint
    Ratajczak M, Ratajczak D, Pędziwiatr D. Extracellular Microvesicles (ExMVs) in Cell to Cell Communication: A Role of Telocytes. Adv Exp Med Biol. 2016;913:41-49 pubmed
    ..Mounting evidence accumulates that telocytes mediate several of their biological effects in several organs by releasing ExMVs enriched in mRNA, miRNA, proteins, and several biological mediators to the target cells. ..
  17. request reprint
    Ratajczak M. Igf2-H19, an imprinted tandem gene, is an important regulator of embryonic development, a guardian of proliferation of adult pluripotent stem cells, a regulator of longevity, and a 'passkey' to cancerogenesis. Folia Histochem Cytobiol. 2012;50:171-9 pubmed
    ..In this review, we will discuss the biological consequences of changes in Igf2-H19 expression. ..
  18. Słuczanowska Głąbowska S, Laszczyńska M, Piotrowska K, Grabowska M, Grymuła K, Ratajczak M. Caloric restriction increases ratio of estrogen to androgen receptors expression in murine ovaries--potential therapeutic implications. J Ovarian Res. 2015;8:57 pubmed publisher
    ..Thus our observation shed more light on a role of beneficial effect of calorie restriction on female reproduction. ..
  19. Ratajczak M, Zuba Surma E, Kucia M, Poniewierska A, Suszynska M, Ratajczak J. Pluripotent and multipotent stem cells in adult tissues. Adv Med Sci. 2012;57:1-17 pubmed publisher
    ..These different populations of stem cells will be reviewed in this paper...
  20. request reprint
    Ratajczak M, Reca R, Wysoczynski M, Yan J, Ratajczak J. Modulation of the SDF-1-CXCR4 axis by the third complement component (C3)--implications for trafficking of CXCR4+ stem cells. Exp Hematol. 2006;34:986-95 pubmed
    ..In this review we will focus on interactions between the SDF-1-CXCR4 axis and the C3 cleavage fragments in a model of mobilization, trafficking, and homing of hematopoietic stem/progenitor cells (HSPC). ..
  21. Ratajczak M, Zuba Surma E, Wysoczynski M, Wan W, Ratajczak J, Wojakowski W, et al. Hunt for pluripotent stem cell -- regenerative medicine search for almighty cell. J Autoimmun. 2008;30:151-62 pubmed publisher
    ..This review will summarize current approaches and limitations to isolate PSC from adult tissues or, alternatively, to generate it by nuclear reprogramming from already differentiated somatic cells...
  22. Ratajczak M, Shin D, Liu R, Marlicz W, Tarnowski M, Ratajczak J, et al. Epiblast/germ line hypothesis of cancer development revisited: lesson from the presence of Oct-4+ cells in adult tissues. Stem Cell Rev. 2010;6:307-16 pubmed publisher
    ..However, we are aware that this working hypothesis requires further direct experimental confirmation...
  23. Ratajczak M, Lee H, Wysoczynski M, Wan W, Marlicz W, Laughlin M, et al. Novel insight into stem cell mobilization-plasma sphingosine-1-phosphate is a major chemoattractant that directs the egress of hematopoietic stem progenitor cells from the bone marrow and its level in peripheral blood increases during mobilization du. Leukemia. 2010;24:976-85 pubmed publisher
    ..We conclude and propose a new paradigm that S1P is a crucial chemoattractant for BM-residing HSPCs and that CC through MAC induces the release of S1P from erythrocytes for optimal egress/mobilization of HSPCs. ..
  24. Ratajczak M, Ratajczak J. Extracellular Microvesicles as Game Changers in Better Understanding the Complexity of Cellular Interactions-From Bench to Clinical Applications. Am J Med Sci. 2017;354:449-452 pubmed publisher
    ..At the same time, there are ongoing attempts to employ them as delivery vehicles for drugs as well as therapeutics in regenerative medicine, oncology and immunotherapy. ..
  25. Bujko K, Rzeszotek S, Hoehlig K, Yan J, Vater A, Ratajczak M. Signaling of the Complement Cleavage Product Anaphylatoxin C5a Through C5aR (CD88) Contributes to Pharmacological Hematopoietic Stem Cell Mobilization. Stem Cell Rev. 2017;13:793-800 pubmed publisher
  26. Sielatycka K, Poniewierska Baran A, Nurek K, Torbe A, Ratajczak M. Novel View on Umbilical Cord Blood and Maternal Peripheral Blood-an Evidence for an Increase in the Number of Circulating Stem Cells on Both Sides of the Fetal-Maternal Circulation Barrier. Stem Cell Rev. 2017;13:774-780 pubmed publisher
    ..Based on this observation, we propose that delivery stress is associated with an increase in the number of circulating stem cells, not only on the fetal side but also on the maternal side of the fetal-maternal circulatory barrier. ..
  27. Ratajczak M, Suszynska M, Borkowska S, Ratajczak J, Schneider G. The role of sphingosine-1 phosphate and ceramide-1 phosphate in trafficking of normal stem cells and cancer cells. Expert Opin Ther Targets. 2014;18:95-107 pubmed publisher
    ..However, additional evidence has accumulated that, in addition to these peptide-based chemoattractants, an important role in cell migration is played by bioactive lipids...
  28. request reprint
    Ratajczak J, Reca R, Kucia M, Majka M, Allendorf D, Baran J, et al. Mobilization studies in mice deficient in either C3 or C3a receptor (C3aR) reveal a novel role for complement in retention of hematopoietic stem/progenitor cells in bone marrow. Blood. 2004;103:2071-8 pubmed
    ..The C3a-C3aR axis may prevent an uncontrolled release of HSPCs into peripheral blood. These data further suggest that the C3aR antagonist SB 290157 could be developed as a drug to mobilize HSPCs for transplantation...
  29. Maj M, Schneider G, Ratajczak J, Suszynska M, Kucia M, Ratajczak M. The cell cycle- and insulin-signaling-inhibiting miRNA expression pattern of very small embryonic-like stem cells contributes to their quiescent state. Exp Biol Med (Maywood). 2015;240:1107-11 pubmed publisher
    ..These observations are important for understanding the biology of these cells and for developing efficient ex vivo expansion strategies for VSELs isolated from adult tissues. ..
  30. Heo J, Lim J, Lee S, Jeong J, Kang H, Kim Y, et al. Sirt1 Regulates DNA Methylation and Differentiation Potential of Embryonic Stem Cells by Antagonizing Dnmt3l. Cell Rep. 2017;18:1930-1945 pubmed publisher
    ..This study sheds light on mechanisms that restrain DNA methylation of developmentally vital genes operating in ESCs. ..
  31. Adamiak M, Suszynska M, Abdel Latif A, Abdelbaset Ismail A, Ratajczak J, Ratajczak M. The Involvment of Hematopoietic-Specific PLC -β2 in Homing and Engraftment of Hematopoietic Stem/Progenitor Cells. Stem Cell Rev. 2016;12:613-620 pubmed
    ..This decrease in migration of HSPCs can be explained by impaired calcium release in PLC-β2-KO mice and a high baseline level of heme oxygenase 1 (HO-1), an enzyme that negatively regulates cell migration. ..