Milan Raska

Summary

Affiliation: University of Alabama at Birmingham
Country: USA

Publications

  1. pmc Glycosylation patterns of HIV-1 gp120 depend on the type of expressing cells and affect antibody recognition
    Milan Raska
    Department of Immunology, Palacky University in Olomouc, Olomouc 77100, Czech Republic
    J Biol Chem 285:20860-9. 2010
  2. ncbi request reprint Candidiasis--do we need to fight or to tolerate the Candida fungus?
    M Raska
    Department of Immunology, Medical Faculty, Palacky University, 772 00 Olomouc, Czechia
    Folia Microbiol (Praha) 52:297-312. 2007
  3. pmc Delivery of DNA HIV-1 vaccine to the liver induces high and long-lasting humoral immune responses
    Milan Raska
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294 2170, USA
    Vaccine 26:1541-51. 2008
  4. doi request reprint Systemic and mucosal immunization with Candida albicans hsp90 elicits hsp90-specific humoral response in vaginal mucosa which is further enhanced during experimental vaginal candidiasis
    Milan Raska
    Department of Immunology, Palacky University in Olomouc, Olomouc, Czech Republic
    Med Mycol 46:411-20. 2008
  5. doi request reprint Involvement of envelope-glycoprotein glycans in HIV-1 biology and infection
    Milan Raska
    Department of Immunology, Palacky University in Olomouc, Hnevotinska 3, 772 00 Olomouc, Czech Republic
    Arch Immunol Ther Exp (Warsz) 58:191-208. 2010
  6. doi request reprint Prevention of lyme disease: promising research or sisyphean task?
    Michal Krupka
    Department of Immunology, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Czech Republic
    Arch Immunol Ther Exp (Warsz) 59:261-75. 2011
  7. ncbi request reprint Isolation and purification of recombinant outer surface protein C (rOspC) of Borrelia burgdorferi sensu lato
    Michal Krupka
    Department of Immunology, Palacky University, Olomouc, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 149:261-4. 2005
  8. ncbi request reprint IgA nephropathy: molecular mechanisms of the disease
    Jiri Mestecky
    Department of Microbiology, University of Alabama at Birmingham, AL 35294, USA
    Annu Rev Pathol 8:217-40. 2013
  9. pmc Production of N-acetylgalactosaminyl-transferase 2 (GalNAc-T2) fused with secretory signal Igκ in insect cells
    Milada Horynova
    Department of Immunology, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic
    Protein Expr Purif 81:175-80. 2012
  10. doi request reprint Aberrant glycosylation of IgA1 and anti-glycan antibodies in IgA nephropathy: role of mucosal immune system
    Jan Novak
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Adv Otorhinolaryngol 72:60-3. 2011

Detail Information

Publications29

  1. pmc Glycosylation patterns of HIV-1 gp120 depend on the type of expressing cells and affect antibody recognition
    Milan Raska
    Department of Immunology, Palacky University in Olomouc, Olomouc 77100, Czech Republic
    J Biol Chem 285:20860-9. 2010
    ..These results indicate that gp120 glycans contribute to antibody reactivity and should be considered in HIV-1 vaccine design...
  2. ncbi request reprint Candidiasis--do we need to fight or to tolerate the Candida fungus?
    M Raska
    Department of Immunology, Medical Faculty, Palacky University, 772 00 Olomouc, Czechia
    Folia Microbiol (Praha) 52:297-312. 2007
    ..This balance is absolutely distinct during each candidiasis form and it is crucial to describe and understand the basic principles before designing new therapeutic and/or preventive approaches...
  3. pmc Delivery of DNA HIV-1 vaccine to the liver induces high and long-lasting humoral immune responses
    Milan Raska
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294 2170, USA
    Vaccine 26:1541-51. 2008
    ..7% of CD8(+) splenocytes after in vitro stimulation with HIV-1 envelope peptides. These results demonstrate that DNA vaccines targeted to tissues with high proteosynthetic activity, such as the liver, results in enhanced immune responses...
  4. doi request reprint Systemic and mucosal immunization with Candida albicans hsp90 elicits hsp90-specific humoral response in vaginal mucosa which is further enhanced during experimental vaginal candidiasis
    Milan Raska
    Department of Immunology, Palacky University in Olomouc, Olomouc, Czech Republic
    Med Mycol 46:411-20. 2008
    ....
  5. doi request reprint Involvement of envelope-glycoprotein glycans in HIV-1 biology and infection
    Milan Raska
    Department of Immunology, Palacky University in Olomouc, Hnevotinska 3, 772 00 Olomouc, Czech Republic
    Arch Immunol Ther Exp (Warsz) 58:191-208. 2010
    ....
  6. doi request reprint Prevention of lyme disease: promising research or sisyphean task?
    Michal Krupka
    Department of Immunology, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Czech Republic
    Arch Immunol Ther Exp (Warsz) 59:261-75. 2011
    ..burgdorferi sensu stricto. Recent data indicate that a broadly effective vaccine will to be composed of a mixture of several antigens or multiple epitopes...
  7. ncbi request reprint Isolation and purification of recombinant outer surface protein C (rOspC) of Borrelia burgdorferi sensu lato
    Michal Krupka
    Department of Immunology, Palacky University, Olomouc, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 149:261-4. 2005
    ..For optimalization of protein purification's yield we used immobilized metal ion affinity chromatography (IMAC) under different conditions. The greatest efficiency was achieved by using of HiTrap Chelating Column under native conditions...
  8. ncbi request reprint IgA nephropathy: molecular mechanisms of the disease
    Jiri Mestecky
    Department of Microbiology, University of Alabama at Birmingham, AL 35294, USA
    Annu Rev Pathol 8:217-40. 2013
    ....
  9. pmc Production of N-acetylgalactosaminyl-transferase 2 (GalNAc-T2) fused with secretory signal Igκ in insect cells
    Milada Horynova
    Department of Immunology, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic
    Protein Expr Purif 81:175-80. 2012
    ..In conclusion, murine immunoglobulin kappa secretory signal was used for production of secreted enzymatically active GalNAc-T2 in insect baculovirus expression system...
  10. doi request reprint Aberrant glycosylation of IgA1 and anti-glycan antibodies in IgA nephropathy: role of mucosal immune system
    Jan Novak
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Adv Otorhinolaryngol 72:60-3. 2011
    ..The aberrant glycosylation of IgA1 may reflect abnormal mucosal immune responses to infections of the upper respiratory tract in genetically predisposed individuals...
  11. ncbi request reprint IgA nephropathy and Henoch-Schoenlein purpura nephritis: aberrant glycosylation of IgA1, formation of IgA1-containing immune complexes, and activation of mesangial cells
    Jan Novak
    University of Alabama at Birmingham, Department of Microbiology, Birmingham, AL 35294, USA
    Contrib Nephrol 157:134-8. 2007
    ..In summary, we identified the neoepitopes on IgA1 responsible for formation of the pathogenic immune complexes. These studies may lead to development of noninvasive diagnostic assays and future disease-specific therapy...
  12. ncbi request reprint DNA vaccines: are they still just a powerful tool for the future?
    Jana Belakova
    Department of Immunology, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic
    Arch Immunol Ther Exp (Warsz) 55:387-98. 2007
    ..Finally, the prospects of DNA vaccination and ongoing clinical trials with various DNA vaccines are discussed...
  13. pmc Antibody-mediated protection and the mucosal immune system of the genital tract: relevance to vaccine design
    Jiri Mestecky
    Department of Microbiology, University of Alabama at Birmingham, AL 35294 2170, USA
    J Reprod Immunol 85:81-5. 2010
    ..The approaches have been explored to a limited extent in humans...
  14. ncbi request reprint Novel modification of growth medium enables efficient E. coli expression and simple purification of an endotoxin-free recombinant murine hsp70 protein
    Katerina Zachova
    Department of Immunology, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Trida Svobody, Olomouc
    J Microbiol Biotechnol 19:727-33. 2009
    ..In summary, a simple modification of growth medium allowed production of recombinant mouse hsp70 in high yield and purity, thus compatible with immunological studies. This protocol may be useful for production of other His...
  15. pmc Aberrant O-glycosylation and anti-glycan antibodies in an autoimmune disease IgA nephropathy and breast adenocarcinoma
    Milada Stuchlová Horynová
    Department of Immunology, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 77515, Olomouc, Czech Republic
    Cell Mol Life Sci 70:829-39. 2013
    ..In breast cancer, elevated levels of antibodies recognizing aberrant MUC1 are associated with better outcome, whereas in IgA nephropathy, the antibodies recognizing aberrant IgA1 are part of the pathogenetic process...
  16. ncbi request reprint Biological aspects of Lyme disease spirochetes: unique bacteria of the Borrelia burgdorferi species group
    Michal Krupka
    Department of Immunology, Faculty of Medicine, Palacky University, Olomouc, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 151:175-86. 2007
    ..Many of the biological features of Borrelia are unique in prokaryotes and very interesting not only from the medical viewpoint but also from the view of molecular biology...
  17. pmc Identification and characterization of CMP-NeuAc:GalNAc-IgA1 alpha2,6-sialyltransferase in IgA1-producing cells
    Milan Raska
    University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Mol Biol 369:69-78. 2007
    ..These data are the first evidence that human cells that lack ST6-GalNAcI can sialylate core 1 GalNAc-Ser/Thr...
  18. ncbi request reprint Preparation and purification of recombinant outer surface protein A (rOspA) of Borrelia burgdorferi sensu stricto and Borrelia afzelii
    Jana Belakova
    Department of Immunology, Palacky University, Olomouc, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 149:257-9. 2005
    ..Our aim was to obtain rOspA protein in a purity and quantity desirable for immunization of experimental animals. rOspA is currently the most developed, molecularly-defined vaccine candidate for the prevention of Lyme borreliosis...
  19. doi request reprint Isolation of recombinant cysteine dioxygenase protein from Trichophyton mentagrophytes
    Alena Kasperova
    Department of Immunology, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Czech Republic
    Mycoses 54:e456-62. 2011
    ..Identification of T. mentagrophytes CDO provides indispensable tools for future studies of dermatophyte pathogenicity and development of new approaches for prevention and therapy...
  20. pmc Enzymatic sialylation of IgA1 o-glycans: implications for studies of IgA nephropathy
    Kazuo Takahashi
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama, United States of America Department of Nephrology, Fujita Health University School of Medicine, Toyoake, Japan
    PLoS ONE 9:e99026. 2014
    ..Thus, enzymatic sialylation offers a useful model to test the role of NeuAc in reactivities of the clustered O-glycans with lectins. ..
  21. doi request reprint Enhancement of immune response towards non-lipidized Borrelia burgdorferi recombinant OspC antigen by binding onto the surface of metallochelating nanoliposomes with entrapped lipophilic derivatives of norAbuMDP
    Michal Krupka
    Department of Immunology, Palacky University in Olomouc, Czech Republic
    J Control Release 160:374-81. 2012
    ..Both adjuvants exerted a high adjuvant effect comparable or better than MDP and proved themselves as nonpyrogenic...
  22. ncbi request reprint Development of the primer set for the detection of the hsp60 gene in Trichophyton mentagrophytes cDNA
    P Kopecek
    Department of Biology, Medical Faculty, Palacky University, Olomouc, Czech Republic
    Folia Microbiol (Praha) 44:401-5. 1999
    ..cerevisiae. Similar fragments detected in both PCR's imply the possible future use of the developed primer set for the detection of the hsp60 gene in other fungal species...
  23. ncbi request reprint Isolation and characterization of an immunogenic fragment of heat shock protein 60 from Trichophyton mentagrophytes
    M Raska
    Department of Immunology, Faculty of Medicine, Palacky University, Olomouc, Czech Republic
    Mycoses 47:482-90. 2004
    ..High immunogenicity of r-hsp60-TM664 was proven after subcutaneous immunization of mice. Immunized mouse sera recognized r-hsp60-TM664 on Western blots as well as hsp60 from mouse liver lysate and lysate of Candida albicans...
  24. ncbi request reprint Comparison of protective effect of protein and DNA vaccines hsp90 in murine model of systemic candidiasis
    M Raska
    Department of Immunology, Faculty of Medicine, Palacky University, Olomouc, Czechia
    Folia Microbiol (Praha) 50:77-82. 2005
    ..d. protein and DNA vaccination approaches. We stressed the importance of specific humoral immunity in the mouse model of systemic candidiasis...
  25. pmc Humoral immune responses to HIV in the mucosal secretions and sera of HIV-infected women
    Jiri Mestecky
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL, USA Institute of Immunology and Microbiology, Faculty of Medicine, Charles University, Prague, Czech Republic
    Am J Reprod Immunol 71:600-7. 2014
    ....
  26. doi request reprint The possible role of dermatophyte cysteine dioxygenase in keratin degradation
    Alena Kasperova
    Department of Immunolgy, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Olomouc, Czech Republic
    Med Mycol 51:449-54. 2013
    ..Because sulphite cleaves disulphide bridges as a first unavoidable step in keratinolysis, it is hypothesized that in dermatophytes, CDO is a virulence factor crucial for keratin degradation...
  27. ncbi request reprint [Strategic dominance of the mucosal immune system in the defence and tolerance]
    Jiri Mestecky
    University of Alabama at Birmingham, Department of Microbiology, USA
    Cas Lek Cesk 150:480-8. 2011
    ..Further it aims to elucidate conditions critical for elicitation of mucosal tolerance...
  28. ncbi request reprint Detection of antigens in mycelial and in arthroconidial phases of Trichophyton mentagrophytes
    P Kopecek
    Department of Biology, Medical Faculty, Palacky University, Olomouc, Czech Republic
    Folia Microbiol (Praha) 43:702-6. 1998
    ..5, 18.5 kDa, in arthroconidia 108, 92, 88, 66, 56, 41, 39, 19.5 kDa. The results suggest the participation of some heat shock associated proteins of T. mentagrophytes in host immune response against mycotic infection...
  29. ncbi request reprint Heat shock proteins in autoimmune diseases
    Milan Raska
    Department of Immunology, Palacky University in Olomouc, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 149:243-9. 2005
    ....