Research Topics
Genomes and Genes | Madhavi J RaneSummaryAffiliation: University of Louisville Country: USA Publications
Research Grants
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Publications
Hsp27 regulates Akt activation and polymorphonuclear leukocyte apoptosis by scaffolding MK2 to Akt signal complexRui Wu
Department of Medicine, University of Louisville, Louisville, KY 40202, USA
J Biol Chem 282:21598-608. 2007..These studies collectively define a novel role of Hsp27 in regulating Akt activation and cellular apoptosis by mediating interaction between Akt and its upstream activator MK2...- p38 Kinase-dependent MAPKAPK-2 activation functions as 3-phosphoinositide-dependent kinase-2 for Akt in human neutrophilsM J Rane
Department of Medicine, University of Louisville Health Sciences Center and the Veterans Affairs Medical Center, Louisville, Kentucky 40202, USA
J Biol Chem 276:3517-23. 2001..We conclude that Akt exists in a signaling complex containing p38 kinase, MK2, and Hsp27 and that p38-dependent MK2 activation functions as PDK2 in human neutrophils... - Gamma-amino butyric acid type B receptors stimulate neutrophil chemotaxis during ischemia-reperfusionMadhavi J Rane
Department of Medicine, University of Louisville, Louisville, KY 40202, USA
J Immunol 174:7242-9. 2005..The GABA(B)R potentially plays a significant role in the inflammatory response and neutrophil-dependent ischemia-reperfusion injury such as stroke... 
Proteomic identification of 14-3-3zeta as a mitogen-activated protein kinase-activated protein kinase 2 substrate: role in dimer formation and ligand bindingDavid W Powell
Departments of Biochemistry and Molecular Biology, University of Louisville, Louisville, KY 40202, USA
Mol Cell Biol 23:5376-87. 2003..These data suggest that MAPKAPK2-mediated phosphorylation regulates 14-3-3zeta functions, and this MAPKAPK2 activity may represent a novel pathway mediating p38 MAPK-dependent inflammation...- MAPK-activated protein kinase-2 participates in p38 MAPK-dependent and ERK-dependent functions in human neutrophilsPatricia Y Coxon
Department of Medicine, University of Louisville Health Sciences Center, Louisville, KY 40202, USA
Cell Signal 15:993-1001. 2003..ERK phosphorylated MK2 in vitro and activated MK2 in f-methionyl-leucyl-phenylalanine (FMLP)-stimulated neutrophils. These data suggest that MK2 mediates both ERK- and p38 MAPK-dependent neutrophil responses... - Heat shock protein 27 controls apoptosis by regulating Akt activationMadhavi J Rane
Department of Medicine, University of Louisville, Louisville, Kentucky 40202 1764, USA
J Biol Chem 278:27828-35. 2003..Our results define a new mechanism by which Hsp27 regulates apoptosis, through control of Akt activity... - Identification of the p16-Arc subunit of the Arp 2/3 complex as a substrate of MAPK-activated protein kinase 2 by proteomic analysisSaurabh Singh
Department of Biochemistry and Molecular Biology, Medicine, and Pharmacology, University of Louisville, KY 40202, USA
J Biol Chem 278:36410-7. 2003..The ability of MAPKAPK2 to phosphorylate one isoform of p16-Arc suggests a possible mechanism by which the p38 MAPK cascade regulates remodeling of the actin cytoskeleton... - Heat shock protein 27 regulates neutrophil chemotaxis and exocytosis through two independent mechanismsNeelakshi R Jog
Department of Microbiology and Immunology, University of Louisville School of Medicine, Louisville, KY 40202, USA
J Immunol 178:2421-8. 2007..Phosphorylation of Hsp27 regulates chemotaxis, but not exocytosis, independent of regulation of actin reorganization... - Ouabain stimulates protein kinase B (Akt) phosphorylation in opossum kidney proximal tubule cells through an ERK-dependent pathwaySyed J Khundmiri
Kidney Disease Program, Univ of Louisville, 570 S Preston St, South POD 102, Louisville, KY 40202, USA
Am J Physiol Cell Physiol 293:C1171-80. 2007..These findings suggest that ouabain-stimulated ERK phosphorylation is required for Akt phosphorylation on Ser(473), cell proliferation, and stimulation of Na(+)/K(+)-ATPase-mediated (86)Rb uptake in OK cells... - Akt phosphorylates p47phox and mediates respiratory burst activity in human neutrophilsQingdan Chen
Department of Medicine, University of Louisville, Louisville, KY 40202, USA
J Immunol 170:5302-8. 2003..Inhibition of Akt activity also inhibited fMLP-stimulated neutrophil chemotaxis. We propose that Akt mediates PI-3K-dependent p47(phox) phosphorylation, which contributes to respiratory burst activity in human neutrophils... - Akt-mediated valosin-containing protein 97 phosphorylation regulates its association with ubiquitinated proteinsJon B Klein
Core Proteomics Laboratory, University of Louisville, Kentucky 40202, USA
J Biol Chem 280:31870-81. 2005..We concluded that Akt phosphorylates VCP in vitro and in vivo, and VCP phosphorylation releases it from ubiquitinated substrate protein(s) possibly allowing ubiquitinated protein(s) to be degraded by the proteosome... 
Granule exocytosis contributes to priming and activation of the human neutrophil respiratory burstSilvia M Uriarte
Department of Medicine, University of Louisville, Louisville, KY 40202, USA
J Immunol 187:391-400. 2011....- Interplay between Akt and p38 MAPK pathways in the regulation of renal tubular cell apoptosis associated with diabetic nephropathyMadhavi J Rane
Department of Medicine, University of Louisville, Louisville, Kentucky 40202, USA
Am J Physiol Renal Physiol 298:F49-61. 2010..Thus PTEN or Hsp25 could serve as potential therapeutic targets to modulate Akt activation and control p38 MAPK-mediated diabetic complications... - The actin cytoskeleton regulates exocytosis of all neutrophil granule subsetsNeelakshi R Jog
Department of Microbiology and Immunology, University of Louisville School of Medicine, Louisville, Kentucky 40202, USA
Am J Physiol Cell Physiol 292:C1690-700. 2007..Differential association of actin with the four granule subsets was not associated with graded exocytosis... - Insulin-response element-binding protein 1: a novel Akt substrate involved in transcriptional action of insulinBetty C Villafuerte
Division of Endocrinology and Metabolism, Department of Medicine, University of Louisville, Louisville, KY 40202, USA
J Biol Chem 279:36650-9. 2004..Identification of a novel target of Akt that appears to mediate signals specific for insulin action should provide further insight into the mechanism of insulin action at the genomic level... - Myeloid-related protein-14 is a p38 MAPK substrate in human neutrophilsGeorge Lominadze
Department of Biochemistry and Molecular Biology, University of Louisville School of Medicine, KY 40202, USA
J Immunol 174:7257-67. 2005..Direct phosphorylation of the MRP-14/MRP-8 complex by p38 MAPK increased actin binding in vitro by 2-fold. These results indicate that MRP-14 is a potential mediator of p38 MAPK-dependent functional responses in human neutrophils... - Regulation of insulin-response element binding protein-1 in obesity and diabetes: potential role in impaired insulin-induced gene transcriptionJaspreet Chahal
Department of Medicine, Division of Endocrinology and Metabolism, University of Louisville School of Medicine, 580 South Preston Street, Delia Baxter Building, Room 119E, Louisville, Kentucky 40202, USA
Endocrinology 149:4829-36. 2008..Our study supports the notion that IRE-BP1 may be relevant to the action of insulin in vivo and may play a role in the development of insulin resistance and diabetes... - A proteomic screen identified stress-induced chaperone proteins as targets of Akt phosphorylation in mesangial cellsMichelle T Barati
Department of Medicine, University of Louisville, Louisville, Kentucky 40202, USA
J Proteome Res 5:1636-46. 2006..The data suggest that Akt-mediated phosphorylation of stress-induced chaperones represents a mechanism for regulation of chaperone function during mesangial cell responses to physiologic and pathologic stimuli... - Parathyroid hormone regulation of NA+,K+-ATPase requires the PDZ 1 domain of sodium hydrogen exchanger regulatory factor-1 in opossum kidney cellsSyed Jalal Khundmiri
Department of Medicine, University of Louisville, Kidney Disease Program, Louisville, KY 40202, USA
J Am Soc Nephrol 16:2598-607. 2005..These results demonstrate that NHERF-1 is necessary for PTH-mediated inhibition of Na+,K+-ATPase activity and that the inhibition is mediated through the PDZ1, not PDZ2, domain... - Identification of 14-3-3zeta as a protein kinase B/Akt substrateDavid W Powell
Department of Biochemistry and Molecular Biology, University of Louisville, Louisville, Kentucky 40202 1718, USA
J Biol Chem 277:21639-42. 2002..These data indicate that PKB/Akt phosphorylates Ser-58 on 14-3-3zeta both in vitro and in intact cells. The functional relevance of this phosphorylation remains to be determined... - Application of proteomics to neutrophil biologyGregory C Luerman
Department of Biochemistry and Molecular Biology, University of Louisville, Louisville, KY 40202, USA
J Proteomics 73:552-61. 2010.... - Role of oxidative stress in geldanamycin-induced cytotoxicity and disruption of Hsp90 signaling complexChristina B Clark
Department of Pharmacology and Toxicology, University of Louisville, Louisville, KY 40292, USA
Free Radic Biol Med 47:1440-9. 2009..These differences between MEN- and GA-induced cytotoxicity may allow more specific targeting of cancer cells... - Ouabain induces cell proliferation through calcium-dependent phosphorylation of Akt (protein kinase B) in opossum kidney proximal tubule cellsSyed J Khundmiri
Department of Medicine, University of Louisville, Louisville, Kentucky 40202, USA
Am J Physiol Cell Physiol 291:C1247-57. 2006..Taken together, the results point to a role for CCE and Akt phosphorylation, in response to low concentrations of ouabain, that increase the rate of cell proliferation without inhibiting Na(+)-K(+)-ATPase-mediated ion transport... - Regulation of neutrophil apoptosis by modulation of PKB/Akt activationMadhavi J Rane
Department of Medicine, University of Louisville, Louisville, Kentucky, Louisville, Kentucky, USA
Front Biosci 14:2400-12. 2009..Various substrates of PKB/Akt as well as positive and negative regulators of PKB/Akt activation are discussed which in turn inhibit or enhance cellular survival... - Parathyroid hormone regulation of type II sodium-phosphate cotransporters is dependent on an A kinase anchoring proteinSyed Jalal Khundmiri
Kidney Disease Program, Department of Medicine, University of Louisville, Kentucky 40202, USA
J Biol Chem 278:10134-41. 2003..We conclude that parathyroid hormone inhibits proximal renal tubule sodium-phosphate cotransport through a signaling complex dependent upon an AKAP... - Prevention of diabetic nephropathy by sulforaphane: possible role of Nrf2 upregulation and activationWenpeng Cui
Department of Nephrology, The Second Hospital Jilin University, Changchun 130041, China
Oxid Med Cell Longev 2012:821936. 2012..However, the SFN preventive effect on diabetes-induced renal pathogeneses is not sustained, suggesting the requirement of continual use of SFN for its sustained effect...
Research Grants
- Role of Hsp27 in regulation of Neutrophil ApoptosisMadhavi Rane; Fiscal Year: 2006..These studies will lead us to new targets for disrupting neutrophil-mediated tissue damage in inflammatory diseases. ..
- Modulation of Neutrophil Apoptosis by Akt-Hsp27 SignalosomeMadhavi Rane; Fiscal Year: 2009..These studies will lead us to new targets for disrupting neutrophil-mediated tissue damage in inflammatory diseases. ..