Madhavi J Rane

Summary

Affiliation: University of Louisville
Country: USA

Publications

  1. ncbi request reprint Hsp27 regulates Akt activation and polymorphonuclear leukocyte apoptosis by scaffolding MK2 to Akt signal complex
    Rui Wu
    Department of Medicine, University of Louisville, Louisville, KY 40202, USA
    J Biol Chem 282:21598-608. 2007
  2. ncbi request reprint p38 Kinase-dependent MAPKAPK-2 activation functions as 3-phosphoinositide-dependent kinase-2 for Akt in human neutrophils
    M J Rane
    Department of Medicine, University of Louisville Health Sciences Center and the Veterans Affairs Medical Center, Louisville, Kentucky 40202, USA
    J Biol Chem 276:3517-23. 2001
  3. ncbi request reprint Gamma-amino butyric acid type B receptors stimulate neutrophil chemotaxis during ischemia-reperfusion
    Madhavi J Rane
    Department of Medicine, University of Louisville, Louisville, KY 40202, USA
    J Immunol 174:7242-9. 2005
  4. ncbi request reprint MAPK-activated protein kinase-2 participates in p38 MAPK-dependent and ERK-dependent functions in human neutrophils
    Patricia Y Coxon
    Department of Medicine, University of Louisville Health Sciences Center, Louisville, KY 40202, USA
    Cell Signal 15:993-1001. 2003
  5. pmc Proteomic identification of 14-3-3zeta as a mitogen-activated protein kinase-activated protein kinase 2 substrate: role in dimer formation and ligand binding
    David W Powell
    Departments of Biochemistry and Molecular Biology, University of Louisville, Louisville, KY 40202, USA
    Mol Cell Biol 23:5376-87. 2003
  6. ncbi request reprint Heat shock protein 27 controls apoptosis by regulating Akt activation
    Madhavi J Rane
    Department of Medicine, University of Louisville, Louisville, Kentucky 40202 1764, USA
    J Biol Chem 278:27828-35. 2003
  7. ncbi request reprint Identification of the p16-Arc subunit of the Arp 2/3 complex as a substrate of MAPK-activated protein kinase 2 by proteomic analysis
    Saurabh Singh
    Department of Biochemistry and Molecular Biology, Medicine, and Pharmacology, University of Louisville, KY 40202, USA
    J Biol Chem 278:36410-7. 2003
  8. ncbi request reprint Heat shock protein 27 regulates neutrophil chemotaxis and exocytosis through two independent mechanisms
    Neelakshi R Jog
    Department of Microbiology and Immunology, University of Louisville School of Medicine, Louisville, KY 40202, USA
    J Immunol 178:2421-8. 2007
  9. pmc Ouabain stimulates protein kinase B (Akt) phosphorylation in opossum kidney proximal tubule cells through an ERK-dependent pathway
    Syed J Khundmiri
    Kidney Disease Program, Univ of Louisville, 570 S Preston St, South POD 102, Louisville, KY 40202, USA
    Am J Physiol Cell Physiol 293:C1171-80. 2007
  10. ncbi request reprint Akt phosphorylates p47phox and mediates respiratory burst activity in human neutrophils
    Qingdan Chen
    Department of Medicine, University of Louisville, Louisville, KY 40202, USA
    J Immunol 170:5302-8. 2003

Research Grants

Detail Information

Publications28

  1. ncbi request reprint Hsp27 regulates Akt activation and polymorphonuclear leukocyte apoptosis by scaffolding MK2 to Akt signal complex
    Rui Wu
    Department of Medicine, University of Louisville, Louisville, KY 40202, USA
    J Biol Chem 282:21598-608. 2007
    ..These studies collectively define a novel role of Hsp27 in regulating Akt activation and cellular apoptosis by mediating interaction between Akt and its upstream activator MK2...
  2. ncbi request reprint p38 Kinase-dependent MAPKAPK-2 activation functions as 3-phosphoinositide-dependent kinase-2 for Akt in human neutrophils
    M J Rane
    Department of Medicine, University of Louisville Health Sciences Center and the Veterans Affairs Medical Center, Louisville, Kentucky 40202, USA
    J Biol Chem 276:3517-23. 2001
    ..We conclude that Akt exists in a signaling complex containing p38 kinase, MK2, and Hsp27 and that p38-dependent MK2 activation functions as PDK2 in human neutrophils...
  3. ncbi request reprint Gamma-amino butyric acid type B receptors stimulate neutrophil chemotaxis during ischemia-reperfusion
    Madhavi J Rane
    Department of Medicine, University of Louisville, Louisville, KY 40202, USA
    J Immunol 174:7242-9. 2005
    ..The GABA(B)R potentially plays a significant role in the inflammatory response and neutrophil-dependent ischemia-reperfusion injury such as stroke...
  4. ncbi request reprint MAPK-activated protein kinase-2 participates in p38 MAPK-dependent and ERK-dependent functions in human neutrophils
    Patricia Y Coxon
    Department of Medicine, University of Louisville Health Sciences Center, Louisville, KY 40202, USA
    Cell Signal 15:993-1001. 2003
    ..ERK phosphorylated MK2 in vitro and activated MK2 in f-methionyl-leucyl-phenylalanine (FMLP)-stimulated neutrophils. These data suggest that MK2 mediates both ERK- and p38 MAPK-dependent neutrophil responses...
  5. pmc Proteomic identification of 14-3-3zeta as a mitogen-activated protein kinase-activated protein kinase 2 substrate: role in dimer formation and ligand binding
    David W Powell
    Departments of Biochemistry and Molecular Biology, University of Louisville, Louisville, KY 40202, USA
    Mol Cell Biol 23:5376-87. 2003
    ..These data suggest that MAPKAPK2-mediated phosphorylation regulates 14-3-3zeta functions, and this MAPKAPK2 activity may represent a novel pathway mediating p38 MAPK-dependent inflammation...
  6. ncbi request reprint Heat shock protein 27 controls apoptosis by regulating Akt activation
    Madhavi J Rane
    Department of Medicine, University of Louisville, Louisville, Kentucky 40202 1764, USA
    J Biol Chem 278:27828-35. 2003
    ..Our results define a new mechanism by which Hsp27 regulates apoptosis, through control of Akt activity...
  7. ncbi request reprint Identification of the p16-Arc subunit of the Arp 2/3 complex as a substrate of MAPK-activated protein kinase 2 by proteomic analysis
    Saurabh Singh
    Department of Biochemistry and Molecular Biology, Medicine, and Pharmacology, University of Louisville, KY 40202, USA
    J Biol Chem 278:36410-7. 2003
    ..The ability of MAPKAPK2 to phosphorylate one isoform of p16-Arc suggests a possible mechanism by which the p38 MAPK cascade regulates remodeling of the actin cytoskeleton...
  8. ncbi request reprint Heat shock protein 27 regulates neutrophil chemotaxis and exocytosis through two independent mechanisms
    Neelakshi R Jog
    Department of Microbiology and Immunology, University of Louisville School of Medicine, Louisville, KY 40202, USA
    J Immunol 178:2421-8. 2007
    ..Phosphorylation of Hsp27 regulates chemotaxis, but not exocytosis, independent of regulation of actin reorganization...
  9. pmc Ouabain stimulates protein kinase B (Akt) phosphorylation in opossum kidney proximal tubule cells through an ERK-dependent pathway
    Syed J Khundmiri
    Kidney Disease Program, Univ of Louisville, 570 S Preston St, South POD 102, Louisville, KY 40202, USA
    Am J Physiol Cell Physiol 293:C1171-80. 2007
    ..These findings suggest that ouabain-stimulated ERK phosphorylation is required for Akt phosphorylation on Ser(473), cell proliferation, and stimulation of Na(+)/K(+)-ATPase-mediated (86)Rb uptake in OK cells...
  10. ncbi request reprint Akt phosphorylates p47phox and mediates respiratory burst activity in human neutrophils
    Qingdan Chen
    Department of Medicine, University of Louisville, Louisville, KY 40202, USA
    J Immunol 170:5302-8. 2003
    ..Inhibition of Akt activity also inhibited fMLP-stimulated neutrophil chemotaxis. We propose that Akt mediates PI-3K-dependent p47(phox) phosphorylation, which contributes to respiratory burst activity in human neutrophils...
  11. ncbi request reprint Akt-mediated valosin-containing protein 97 phosphorylation regulates its association with ubiquitinated proteins
    Jon B Klein
    Core Proteomics Laboratory, University of Louisville, Kentucky 40202, USA
    J Biol Chem 280:31870-81. 2005
    ..We concluded that Akt phosphorylates VCP in vitro and in vivo, and VCP phosphorylation releases it from ubiquitinated substrate protein(s) possibly allowing ubiquitinated protein(s) to be degraded by the proteosome...
  12. pmc Granule exocytosis contributes to priming and activation of the human neutrophil respiratory burst
    Silvia M Uriarte
    Department of Medicine, University of Louisville, Louisville, KY 40202, USA
    J Immunol 187:391-400. 2011
    ....
  13. pmc Interplay between Akt and p38 MAPK pathways in the regulation of renal tubular cell apoptosis associated with diabetic nephropathy
    Madhavi J Rane
    Department of Medicine, University of Louisville, Louisville, Kentucky 40202, USA
    Am J Physiol Renal Physiol 298:F49-61. 2010
    ..Thus PTEN or Hsp25 could serve as potential therapeutic targets to modulate Akt activation and control p38 MAPK-mediated diabetic complications...
  14. pmc Inhibition of neutrophil exocytosis ameliorates acute lung injury in rats
    Silvia M Uriarte
    Department of Medicine, University of Louisville, Louisville, KY, USA
    Shock 39:286-92. 2013
    ..Our results indicate that administration of a TAT-fusion protein that inhibits neutrophil exocytosis reduces in vivo ALI. Targeting neutrophil exocytosis is a potential therapeutic strategy to ameliorate ALI...
  15. ncbi request reprint The actin cytoskeleton regulates exocytosis of all neutrophil granule subsets
    Neelakshi R Jog
    Department of Microbiology and Immunology, University of Louisville School of Medicine, Louisville, Kentucky 40202, USA
    Am J Physiol Cell Physiol 292:C1690-700. 2007
    ..Differential association of actin with the four granule subsets was not associated with graded exocytosis...
  16. ncbi request reprint Insulin-response element-binding protein 1: a novel Akt substrate involved in transcriptional action of insulin
    Betty C Villafuerte
    Division of Endocrinology and Metabolism, Department of Medicine, University of Louisville, Louisville, KY 40202, USA
    J Biol Chem 279:36650-9. 2004
    ..Identification of a novel target of Akt that appears to mediate signals specific for insulin action should provide further insight into the mechanism of insulin action at the genomic level...
  17. ncbi request reprint Myeloid-related protein-14 is a p38 MAPK substrate in human neutrophils
    George Lominadze
    Department of Biochemistry and Molecular Biology, University of Louisville School of Medicine, KY 40202, USA
    J Immunol 174:7257-67. 2005
    ..Direct phosphorylation of the MRP-14/MRP-8 complex by p38 MAPK increased actin binding in vitro by 2-fold. These results indicate that MRP-14 is a potential mediator of p38 MAPK-dependent functional responses in human neutrophils...
  18. pmc Regulation of insulin-response element binding protein-1 in obesity and diabetes: potential role in impaired insulin-induced gene transcription
    Jaspreet Chahal
    Department of Medicine, Division of Endocrinology and Metabolism, University of Louisville School of Medicine, 580 South Preston Street, Delia Baxter Building, Room 119E, Louisville, Kentucky 40202, USA
    Endocrinology 149:4829-36. 2008
    ..Our study supports the notion that IRE-BP1 may be relevant to the action of insulin in vivo and may play a role in the development of insulin resistance and diabetes...
  19. pmc A proteomic screen identified stress-induced chaperone proteins as targets of Akt phosphorylation in mesangial cells
    Michelle T Barati
    Department of Medicine, University of Louisville, Louisville, Kentucky 40202, USA
    J Proteome Res 5:1636-46. 2006
    ..The data suggest that Akt-mediated phosphorylation of stress-induced chaperones represents a mechanism for regulation of chaperone function during mesangial cell responses to physiologic and pathologic stimuli...
  20. ncbi request reprint Identification of 14-3-3zeta as a protein kinase B/Akt substrate
    David W Powell
    Department of Biochemistry and Molecular Biology, University of Louisville, Louisville, Kentucky 40202 1718, USA
    J Biol Chem 277:21639-42. 2002
    ..These data indicate that PKB/Akt phosphorylates Ser-58 on 14-3-3zeta both in vitro and in intact cells. The functional relevance of this phosphorylation remains to be determined...
  21. ncbi request reprint Parathyroid hormone regulation of NA+,K+-ATPase requires the PDZ 1 domain of sodium hydrogen exchanger regulatory factor-1 in opossum kidney cells
    Syed Jalal Khundmiri
    Department of Medicine, University of Louisville, Kidney Disease Program, Louisville, KY 40202, USA
    J Am Soc Nephrol 16:2598-607. 2005
    ..These results demonstrate that NHERF-1 is necessary for PTH-mediated inhibition of Na+,K+-ATPase activity and that the inhibition is mediated through the PDZ1, not PDZ2, domain...
  22. pmc Application of proteomics to neutrophil biology
    Gregory C Luerman
    Department of Biochemistry and Molecular Biology, University of Louisville, Louisville, KY 40202, USA
    J Proteomics 73:552-61. 2010
    ....
  23. pmc Role of oxidative stress in geldanamycin-induced cytotoxicity and disruption of Hsp90 signaling complex
    Christina B Clark
    Department of Pharmacology and Toxicology, University of Louisville, Louisville, KY 40292, USA
    Free Radic Biol Med 47:1440-9. 2009
    ..These differences between MEN- and GA-induced cytotoxicity may allow more specific targeting of cancer cells...
  24. ncbi request reprint Ouabain induces cell proliferation through calcium-dependent phosphorylation of Akt (protein kinase B) in opossum kidney proximal tubule cells
    Syed J Khundmiri
    Department of Medicine, University of Louisville, Louisville, Kentucky 40202, USA
    Am J Physiol Cell Physiol 291:C1247-57. 2006
    ..Taken together, the results point to a role for CCE and Akt phosphorylation, in response to low concentrations of ouabain, that increase the rate of cell proliferation without inhibiting Na(+)-K(+)-ATPase-mediated ion transport...
  25. ncbi request reprint Regulation of neutrophil apoptosis by modulation of PKB/Akt activation
    Madhavi J Rane
    Department of Medicine, University of Louisville, Louisville, Kentucky, Louisville, Kentucky, USA
    Front Biosci (Landmark Ed) 14:2400-12. 2009
    ..Various substrates of PKB/Akt as well as positive and negative regulators of PKB/Akt activation are discussed which in turn inhibit or enhance cellular survival...
  26. ncbi request reprint Parathyroid hormone regulation of type II sodium-phosphate cotransporters is dependent on an A kinase anchoring protein
    Syed Jalal Khundmiri
    Kidney Disease Program, Department of Medicine, University of Louisville, Kentucky 40202, USA
    J Biol Chem 278:10134-41. 2003
    ..We conclude that parathyroid hormone inhibits proximal renal tubule sodium-phosphate cotransport through a signaling complex dependent upon an AKAP...
  27. ncbi request reprint Contrasting Inflammatory Responses in Severe and Non-severe Community-acquired Pneumonia
    Rafael Fernandez-Botran
    Department of Pathology and Laboratory Medicine, University of Louisville School of Medicine, Louisville, KY, USA
    Inflammation 37:1158-66. 2014
    ....
  28. pmc Prevention of diabetic nephropathy by sulforaphane: possible role of Nrf2 upregulation and activation
    Wenpeng Cui
    Department of Nephrology, The Second Hospital Jilin University, Changchun 130041, China
    Oxid Med Cell Longev 2012:821936. 2012
    ..However, the SFN preventive effect on diabetes-induced renal pathogeneses is not sustained, suggesting the requirement of continual use of SFN for its sustained effect...

Research Grants4

  1. Role of Hsp27 in regulation of Neutrophil Apoptosis
    Madhavi Rane; Fiscal Year: 2006
    ..These studies will lead us to new targets for disrupting neutrophil-mediated tissue damage in inflammatory diseases. ..
  2. Modulation of Neutrophil Apoptosis by Akt-Hsp27 Signalosome
    Madhavi Rane; Fiscal Year: 2009
    ..These studies will lead us to new targets for disrupting neutrophil-mediated tissue damage in inflammatory diseases. ..