Parmjeet Randhawa

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. pmc Identification of species-specific and cross-reactive epitopes in human polyomavirus capsids using monoclonal antibodies
    Parmjeet Randhawa
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Gen Virol 90:634-9. 2009
  2. pmc Anti-BK virus activity of nucleoside analogs
    Parmjeet Randhawa
    Division of Transplantation Pathology, Department of Pathology, University of Pittsburgh, Room Medical Center, Pittsburgh, PA 15213, USA
    Antimicrob Agents Chemother 52:1519-21. 2008
  3. pmc Impact of genomic sequence variability on quantitative PCR assays for diagnosis of polyomavirus BK infection
    P Randhawa
    Division of Transplant Pathology, University of Pittsburgh, Department of Pathology, E737 UPMC Montefiore Hospital, 3459 Fifth Ave, Pittsburgh, PA 15213, USA
    J Clin Microbiol 49:4072-6. 2011
  4. pmc BK Virus replication in vitro: limited effect of drugs interfering with viral uptake and intracellular transport
    Parmjeet Randhawa
    Division of Transplantation Pathology, Department of Pathology, University of Pittsburgh, UPMC Montefiore Hospital, Pittsburgh, PA 15213, USA
    Antimicrob Agents Chemother 51:4492-4. 2007
  5. doi request reprint The significance of renal C4d staining in patients with BK viruria, viremia, and nephropathy
    Ibrahim Batal
    Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    Mod Pathol 22:1468-76. 2009
  6. doi request reprint Clinical significance of the distribution of C4d deposits in different anatomic compartments of the allograft kidney
    Ibrahim Batal
    Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    Mod Pathol 21:1490-8. 2008
  7. doi request reprint Measurements of global cell-mediated immunity in renal transplant recipients with BK virus reactivation
    Ibrahim Batal
    Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    Am J Clin Pathol 129:587-91. 2008
  8. doi request reprint Clinicopathologic analysis of patients with BK viruria and rejection-like graft dysfunction
    Ibrahim Batal
    Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    Hum Pathol 40:1312-9. 2009
  9. doi request reprint Antihuman leukocyte antigen–specific antibody strength determined by complement-dependent or solid-phase assays can predict positive donor-specific crossmatches
    Ibrahim Batal
    Departments of Pathology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
    Arch Pathol Lab Med 134:1534-40. 2010
  10. doi request reprint Neutrophilic tubulitis as a marker for urinary tract infection in renal allograft biopsies with C4d deposition
    Gaurav Gupta
    Department of Internal Medicine, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    Transplantation 87:1013-8. 2009

Detail Information

Publications42

  1. pmc Identification of species-specific and cross-reactive epitopes in human polyomavirus capsids using monoclonal antibodies
    Parmjeet Randhawa
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Gen Virol 90:634-9. 2009
    ..7 and JC-7.9) and two of 13 SV40 antibodies (VP1-H2 and VP1-I2) recognized linear buried epitopes common to all three viruses and (iii) SV40 antibody VP1-E5 recognized a linear surface epitope on JCV pseudocapsids...
  2. pmc Anti-BK virus activity of nucleoside analogs
    Parmjeet Randhawa
    Division of Transplantation Pathology, Department of Pathology, University of Pittsburgh, Room Medical Center, Pittsburgh, PA 15213, USA
    Antimicrob Agents Chemother 52:1519-21. 2008
    ....
  3. pmc Impact of genomic sequence variability on quantitative PCR assays for diagnosis of polyomavirus BK infection
    P Randhawa
    Division of Transplant Pathology, University of Pittsburgh, Department of Pathology, E737 UPMC Montefiore Hospital, 3459 Fifth Ave, Pittsburgh, PA 15213, USA
    J Clin Microbiol 49:4072-6. 2011
    ..Physicians need to be cognizant of these effects when interpreting the results of quantitative PCR testing in transplant recipients, particularly if there is a discrepancy between the clinical impression and the measured viral load...
  4. pmc BK Virus replication in vitro: limited effect of drugs interfering with viral uptake and intracellular transport
    Parmjeet Randhawa
    Division of Transplantation Pathology, Department of Pathology, University of Pittsburgh, UPMC Montefiore Hospital, Pittsburgh, PA 15213, USA
    Antimicrob Agents Chemother 51:4492-4. 2007
    ..In vitro studies demonstrated slight antiviral activity for chloroquine and nystatin. A sialic acid derivative, BTB11968, was identified as a lead compound for further development...
  5. doi request reprint The significance of renal C4d staining in patients with BK viruria, viremia, and nephropathy
    Ibrahim Batal
    Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    Mod Pathol 22:1468-76. 2009
    ..A subset of biopsies with BK nephropathy shows tubular basement membrane C4d staining, which correlates with marked viral cytopathic effect...
  6. doi request reprint Clinical significance of the distribution of C4d deposits in different anatomic compartments of the allograft kidney
    Ibrahim Batal
    Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    Mod Pathol 21:1490-8. 2008
    ..Finally, C4d deposits preferentially in lesions of chronic transplant glomerulopathy and arteriolar hyalinosis...
  7. doi request reprint Measurements of global cell-mediated immunity in renal transplant recipients with BK virus reactivation
    Ibrahim Batal
    Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    Am J Clin Pathol 129:587-91. 2008
    ..Within the viruria group, lower ICF values were associated with higher urinary viral load (P= .037). These results show that a decreased ICF test result correlates with active viral replication in kidney transplant recipients...
  8. doi request reprint Clinicopathologic analysis of patients with BK viruria and rejection-like graft dysfunction
    Ibrahim Batal
    Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    Hum Pathol 40:1312-9. 2009
    ..08) and with significant development of viremia after antirejection treatment (5/9 [56%] versus 0/24 [0%] in patients with low VL, P < .001)...
  9. doi request reprint Antihuman leukocyte antigen–specific antibody strength determined by complement-dependent or solid-phase assays can predict positive donor-specific crossmatches
    Ibrahim Batal
    Departments of Pathology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA
    Arch Pathol Lab Med 134:1534-40. 2010
    ..The association of circulating donor-specific antibody (DSA) strength with crossmatch results is of potential interest to predict allograft outcome...
  10. doi request reprint Neutrophilic tubulitis as a marker for urinary tract infection in renal allograft biopsies with C4d deposition
    Gaurav Gupta
    Department of Internal Medicine, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    Transplantation 87:1013-8. 2009
    ..This study evaluated the incidence of UTI in biopsies with concurrent neutrophilic tubulitis and PTC C4d staining...
  11. pmc Putative episodes of T-cell-mediated rejection in patients with sustained BK viruria but no viremia
    Kosuke Masutani
    Division of Transplant Pathology, Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA
    Transplantation 94:43-9. 2012
    ..Polyomavirus BK (BKV) infection characterized by viruria alone is considered to be of little clinical significance, but this issue has not been systematically studied...
  12. pmc Correlates of quantitative measurement of BK polyomavirus (BKV) DNA with clinical course of BKV infection in renal transplant patients
    Parmjeet Randhawa
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    J Clin Microbiol 42:1176-80. 2004
    ..In conclusion, serial measurement of viral loads by quantitative PCR is a useful tool in monitoring the course of BK virus infection. The results should be interpreted in conjunction with the clinical picture and biopsy findings...
  13. ncbi request reprint Quantitation of DNA of polyomaviruses BK and JC in human kidneys
    Parmjeet Randhawa
    Division of Transplantation Pathology, Department of Pathology, University of Pittsburgh, PA 15213, USA
    J Infect Dis 192:504-9. 2005
    ..Because virus in urine can be derived from the kidneys, ureter, or urinary bladder, we evaluated whether measurement of intrarenal concentrations of viral DNA might serve as a more reliable monitoring tool...
  14. ncbi request reprint A comparative study of BK and JC virus infections in organ transplant recipients
    Parmjeet Randhawa
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    J Med Virol 77:238-43. 2005
    ..BKV viremia was seen in kidney but not liver recipients. The mechanisms underlying these notable phenomena remain to be investigated...
  15. doi request reprint Immunohistochemical markers of tissue injury in biopsies with transplant glomerulitis
    Ibrahim Batal
    Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    Hum Pathol 43:69-80. 2012
    ..Mesangial- or interstitial-von Willebrand factor identified cases with more severe microcirculation injury...
  16. pmc Ether lipid ester derivatives of cidofovir inhibit polyomavirus BK replication in vitro
    Parmjeet Randhawa
    Division of Transplantation Pathology, Department of Pathology, University of Pittsburgh Medical Center Montefiore Hospital, 3459 Fifth Ave, Pittsburgh, PA 15213, USA
    Antimicrob Agents Chemother 50:1564-6. 2006
    ..Esterification of cidofovir with hexadecyloxypropyl, octadecyloxyethyl, and oleyloxyethyl groups results in up to a 3-log lowering of the 50% effective concentration and an increased selectivity index...
  17. doi request reprint BK virus-associated urinary bladder carcinoma in transplant recipients: report of 2 cases, review of the literature, and proposed pathogenetic model
    Borislav A Alexiev
    Department of Pathology, University of Maryland Medical Center, Baltimore, MD 21201, USA
    Hum Pathol 44:908-17. 2013
    ..We propose a model of changes ranging from viral reactivation to dysplasia to invasive carcinoma. Clinical vigilance is warranted for early diagnosis of BK polyomavirus-related urothelial malignancies in transplant recipients...
  18. pmc VP-1 quasispecies in human infection with polyomavirus BK
    Chunqing Luo
    Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    J Med Virol 84:152-61. 2012
    ..Quasispecies occur even in healthy subjects with viruria, but evolutionary selection pressure directed at the viral capsid protein (VP-1) is seen only in patients with viremia or nephropathy...
  19. pmc Phylogenetic analysis of polyomavirus BK sequences
    Preety M Sharma
    Division of Transplant Pathology, E737 UPMC Montefiore Hospital, 3459 Fifth Ave, Pittsburgh, PA 15213, USA
    J Virol 80:8869-79. 2006
    ..However, within the kidney transplant population, BKV strains derived from patients with asymptomatic viruria did not show complete separation from strains associated with allograft nephropathy...
  20. doi request reprint Monitoring of human liver and kidney allograft tolerance: a tissue/histopathology perspective
    Anthony J Demetris
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, PA 15213, USA
    Transpl Int 22:120-41. 2009
    ....
  21. ncbi request reprint Effect of leflunomide and cidofovir on replication of BK virus in an in vitro culture system
    Noush Afarin Farasati
    Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    Transplantation 79:116-8. 2005
    ..Cidofovir and Leflunomide are used empirically in the treatment of BK virus nephropathy. The aim of this study is to quantify the antiviral activity of these drugs...
  22. doi request reprint Emerging role of donor-specific anti-human leukocyte antigen antibody determination for clinical management after solid organ transplantation
    Adriana Zeevi
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Hum Immunol 70:645-50. 2009
    ..These cases exemplify the rationale for all patients to be monitored for DSA post-transplantation, with the frequency adjusted based on the individual risk for AMR...
  23. ncbi request reprint Monitoring for polyomavirus BK And JC in urine: comparison of quantitative polymerase chain reaction with urine cytology
    Parmjeet Randhawa
    Division of Transplant Pathology, Department of Pathology, University of Pittsburgh, PA 15213, USA
    Transplantation 79:984-6. 2005
    ..Compared with PCR, urine cytology is a less sensitive technique, which requires morphologically intact cells, and cannot distinguish BKV from JC virus...
  24. pmc Genotyping schemes for polyomavirus BK, using gene-specific phylogenetic trees and single nucleotide polymorphism analysis
    Chunqing Luo
    Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Virol 83:2285-97. 2009
    ..Interstrain genetic recombination between distant loci in the VP1 and LTA areas is not a common event...
  25. pmc Long-term effects of alemtuzumab on regulatory and memory T-cell subsets in kidney transplantation
    Camila Macedo
    Human Immunology Program, Thomas E Starzl Transplantation Institute and Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA
    Transplantation 93:813-21. 2012
    ..It is not fully understood which memory and regulatory T-cell subsets are most affected and how they relate to clinical outcomes...
  26. ncbi request reprint HLA mismatching increases the risk of BK virus nephropathy in renal transplant recipients
    Yehia Awadalla
    Departments of Pathology and Biostatistics, University of Pittsburgh, Pittsburgh
    Am J Transplant 4:1691-6. 2004
    ..We propose that HLA mismatching promotes the development of BKV nephropathy through rejection-related inflammatory processes and heavy immunosuppression which cause virus reactivation and injury of the tubular epithelium...
  27. pmc Validation of BKV large T-antigen ATP-binding site as a target for drug discovery
    Gang Zeng
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA, USA
    Antiviral Res 81:184-7. 2009
    ..Pilot screening of 300 compounds from the Tim Tec ActiTarg K library identified a compound, STO18584, with selectivity index of 19.2...
  28. pmc Cold heparinized lactated Ringers with procaine (HeLP) preservation fluid in 266 living donor kidney transplantations
    Henkie P Tan
    the Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    Transplantation 83:1134-6. 2007
    ..This study documents the effectiveness of cold HeLP as a flushing and short-term preservation fluid for living donor kidney transplantation with excellent results and significant cost benefit because of its low cost...
  29. pmc Kidney transplantation under minimal immunosuppression after pretransplant lymphoid depletion with Thymoglobulin or Campath
    Ron Shapiro
    Thomas E Starzl Transplantation Institute, The University of Pittsburgh Medical Center, PA 15213, USA
    J Am Coll Surg 200:505-15; quiz A59-61. 2005
    ..We have suggested that transplantation outcomes can be improved by modifying the timing and dosage of immunosuppression to facilitate natural mechanisms of alloengraftment and acquired tolerance...
  30. doi request reprint Renal biopsy findings predicting outcome in scleroderma renal crisis
    Ibrahim Batal
    Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    Hum Pathol 40:332-40. 2009
    ..Vascular thrombosis, severe glomerular ischemic collapse, and peritubular capillary C4d deposits in scleroderma renal crisis kidney biopsies correlated with increased risk of failure to recover renal function...
  31. ncbi request reprint A schema for histologic grading of small intestine allograft acute rejection
    Tong Wu
    Department of Pathology, Thomas E Starzl Transplantation Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Transplantation 75:1241-8. 2003
    ..The primary goal of this study was to develop a histologic grading system for the diagnosis of small bowel allograft acute rejection...
  32. pmc Tolerogenic immunosuppression for organ transplantation
    Thomas E Starzl
    Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    Lancet 361:1502-10. 2003
    ..We aimed to apply these principles in recipients of renal and extrarenal organ transplants...
  33. pmc HLA-A2, HLA-B44 and HLA-DR15 are associated with lower risk of BK viremia
    Kosuke Masutani
    Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA, USA
    Nephrol Dial Transplant 28:3119-26. 2013
    ..Human leucocyte antigens (HLAs) modulate immunity to polyomavirus BK (BKV). Identification of HLAs that alter the course of infection will facilitate risk stratification, and customization of pre-emptive intervention strategies...
  34. ncbi request reprint Serum analysis after transplant nephrectomy reveals restricted antibody specificity patterns against structurally defined HLA class I mismatches
    Oyedele A Adeyi
    Division of Transplantation Pathology and CLSI Tissue Typing Laboratory, Department of Pathology, Thomas E Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA, 15261 USA
    Transpl Immunol 14:53-62. 2005
    ..It provides also opportunities for determining the relative immunogenicity of mismatched triplets...
  35. ncbi request reprint Helicobacter pylori may play a contributory role in the pathogenesis of primary sclerosing cholangitis
    Alyssa M Krasinskas
    Department of Pathology, Division of Anatomic Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Dig Dis Sci 52:2265-70. 2007
    ....
  36. ncbi request reprint Viral regulatory region sequence variations in kidney tissue obtained from patients with BK virus nephropathy
    Parmjeet Randhawa
    Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Kidney Int 64:743-7. 2003
    ..We hypothesized that DNA sequence variations in this region play a role in the pathogenesis of BK virus allograft nephropathy (BKVAN)...
  37. ncbi request reprint Pathogenesis and management of polyomavirus infection in transplant recipients
    Eun Jeong Kwak
    Department of Medicine, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA
    Clin Infect Dis 35:1081-7. 2002
    ..Clinical syndromes related to polyomavirus infection are summarized in the present review, and strategies for the management of patients who receive transplants are discussed...
  38. ncbi request reprint Conceptual problems in the diagnosis and therapy of acute rejection in patients with polyomavirus nephropathy
    Parmjeet Randhawa
    Am J Transplant 4:840. 2004
  39. ncbi request reprint Liver biopsy interpretation for causes of late liver allograft dysfunction
    Anthony J Demetris
    Hepatology 44:489-501. 2006
    ..A discussion of differential diagnosis is also presented...
  40. ncbi request reprint Polyomavirus-associated nephropathy in renal transplantation: interdisciplinary analyses and recommendations
    Hans H Hirsch
    Transplantation Virology, Institute for Medical Microbiology and Div Infectious Diseases, Universitatsspital Basel, Switzerland
    Transplantation 79:1277-86. 2005
    ..25-0.33 mg/kg intravenously biweekly) without probenicid should be considered for refractory cases. Retransplantation after renal allograft loss to PVAN remains a treatment option for patients clearing polyomavirus replication...
  41. ncbi request reprint Antibody-mediated rejection criteria - an addition to the Banff 97 classification of renal allograft rejection
    Lorraine C Racusen
    The Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Am J Transplant 3:708-14. 2003
    ..This classification represents a working formulation, to be revisited as additional data accumulate in this important area of renal transplantation...
  42. ncbi request reprint The pathobiology of polyomavirus infection in man
    Parmjeet Randhawa
    University of Pittsburgh Medical Center, Pennsylvania, USA
    Adv Exp Med Biol 577:148-59. 2006
    ..Finally, we present biochemical mechanisms that could potentially explain several key elements of tissue pathology characteristic of BKV mediated damage to human kidney...