Ayyalusamy Ramamoorthy

Summary

Affiliation: University of Michigan
Country: USA

Publications

  1. pmc Probing the transmembrane structure and topology of microsomal cytochrome-p450 by solid-state NMR on temperature-resistant bicelles
    Kazutoshi Yamamoto
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 1055, USA
    Sci Rep 3:2556. 2013
  2. pmc Bacterial curli protein promotes the conversion of PAP248-286 into the amyloid SEVI: cross-seeding of dissimilar amyloid sequences
    Kevin Hartman
    Department of Chemistry, University of Michigan, USA Department of Biophysics, University of Michigan, USA
    Peerj 1:e5. 2013
  3. doi request reprint 2D 1H/1H RFDR and NOESY NMR experiments on a membrane-bound antimicrobial peptide under magic angle spinning
    Ayyalusamy Ramamoorthy
    Biophysics and Department of Chemistry, The University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Phys Chem B 117:6693-700. 2013
  4. doi request reprint Zinc stabilization of prefibrillar oligomers of human islet amyloid polypeptide
    Jeffrey R Brender
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 1055, USA
    Chem Commun (Camb) 49:3339-41. 2013
  5. pmc Solid-state NMR investigation of the membrane-disrupting mechanism of antimicrobial peptides MSI-78 and MSI-594 derived from magainin 2 and melittin
    Ayyalusamy Ramamoorthy
    Biophysics Research Division and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    Biophys J 91:206-16. 2006
  6. ncbi request reprint Nitrogen-14 solid-state NMR spectroscopy of aligned phospholipid bilayers to probe peptide-lipid interaction and oligomerization of membrane associated peptides
    Ayyalusamy Ramamoorthy
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Am Chem Soc 130:11023-9. 2008
  7. pmc Deletion of all cysteines in tachyplesin I abolishes hemolytic activity and retains antimicrobial activity and lipopolysaccharide selective binding
    Ayyalusamy Ramamoorthy
    Department of Chemistry and Biophysics Research Division, The University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    Biochemistry 45:6529-40. 2006
  8. pmc Structure, topology, and tilt of cell-signaling peptides containing nuclear localization sequences in membrane bilayers determined by solid-state NMR and molecular dynamics simulation studies
    Ayyalusamy Ramamoorthy
    Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    Biochemistry 46:965-75. 2007
  9. pmc Cholesterol reduces pardaxin's dynamics-a barrel-stave mechanism of membrane disruption investigated by solid-state NMR
    Ayyalusamy Ramamoorthy
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 1055, USA
    Biochim Biophys Acta 1798:223-7. 2010
  10. pmc Beyond NMR spectra of antimicrobial peptides: dynamical images at atomic resolution and functional insights
    Ayyalusamy Ramamoorthy
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    Solid State Nucl Magn Reson 35:201-7. 2009

Research Grants

  1. Structure and function of antimicrobial peptides
    Ayyalusamy Ramamoorthy; Fiscal Year: 2004
  2. Structural Studies on Membrane-Associate Cytochrome B5 and P450 NMR
    Ayyalusamy Ramamoorthy; Fiscal Year: 2011
  3. Structural Studies on Membrane-Associate Cytochrome B5 and P450 NMR
    Ayyalusamy Ramamoorthy; Fiscal Year: 2010
  4. Structural Studies on Membrane-Associate Cytochrome B5 and P450 NMR
    Ayyalusamy Ramamoorthy; Fiscal Year: 2009
  5. Structure and function of antimicrobial peptides
    Ayyalusamy Ramamoorthy; Fiscal Year: 2007
  6. Purchase of a 500 MHz Solid State NMR Spectrometer
    Ayyalusamy Ramamoorthy; Fiscal Year: 2007
  7. Structure and function of antimicrobial peptides
    Ayyalusamy Ramamoorthy; Fiscal Year: 2006
  8. Structure and function of antimicrobial peptides
    Ayyalusamy Ramamoorthy; Fiscal Year: 2005
  9. Rapid and efficient production of functional Cytochrome P450 enzymatic complex fo
    Ayyalusamy Ramamoorthy; Fiscal Year: 2010

Collaborators

Detail Information

Publications105 found, 100 shown here

  1. pmc Probing the transmembrane structure and topology of microsomal cytochrome-p450 by solid-state NMR on temperature-resistant bicelles
    Kazutoshi Yamamoto
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 1055, USA
    Sci Rep 3:2556. 2013
    ..Our results reveal that the N-terminal region of rabbit cytochrome-P4502B4, that is usually cleaved off to obtain crystal structures, is helical and has a transmembrane orientation with ~17° tilt from the lipid bilayer normal...
  2. pmc Bacterial curli protein promotes the conversion of PAP248-286 into the amyloid SEVI: cross-seeding of dissimilar amyloid sequences
    Kevin Hartman
    Department of Chemistry, University of Michigan, USA Department of Biophysics, University of Michigan, USA
    Peerj 1:e5. 2013
    ..The ability of curli fibers to interact with proteins of dissimilar sequences suggests cross-seeding may be a more general phenomenon than previously supposed...
  3. doi request reprint 2D 1H/1H RFDR and NOESY NMR experiments on a membrane-bound antimicrobial peptide under magic angle spinning
    Ayyalusamy Ramamoorthy
    Biophysics and Department of Chemistry, The University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Phys Chem B 117:6693-700. 2013
    ..This study provides a means to measure residual (1)H-(1)H dipolar couplings for the investigation of structure, dynamics, and aggregation of peptides using a suitable model membrane like micelles or bicelles...
  4. doi request reprint Zinc stabilization of prefibrillar oligomers of human islet amyloid polypeptide
    Jeffrey R Brender
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 1055, USA
    Chem Commun (Camb) 49:3339-41. 2013
    ..Zinc present in secretory granules has been shown to affect this aggregation. A combination of EXAFS, NMR, and AFM experiments shows that the influence of zinc is most likely due to the stabilization of prefibrillar aggregates of hIAPP...
  5. pmc Solid-state NMR investigation of the membrane-disrupting mechanism of antimicrobial peptides MSI-78 and MSI-594 derived from magainin 2 and melittin
    Ayyalusamy Ramamoorthy
    Biophysics Research Division and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    Biophys J 91:206-16. 2006
    ..These results are discussed in light of lipid-peptide interactions leading to the disruption of membrane via either a carpet or a toroidal-type mechanism...
  6. ncbi request reprint Nitrogen-14 solid-state NMR spectroscopy of aligned phospholipid bilayers to probe peptide-lipid interaction and oligomerization of membrane associated peptides
    Ayyalusamy Ramamoorthy
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Am Chem Soc 130:11023-9. 2008
    ..It is inferred that the membrane orientation and oligomerization of the membrane-associated peptides can be measured using (14)N solid-state NMR spectroscopy...
  7. pmc Deletion of all cysteines in tachyplesin I abolishes hemolytic activity and retains antimicrobial activity and lipopolysaccharide selective binding
    Ayyalusamy Ramamoorthy
    Department of Chemistry and Biophysics Research Division, The University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    Biochemistry 45:6529-40. 2006
    ..These properties help to explain that cysteine residues may not contribute to antimicrobial activity and that the loss of hemolytic activity is due to lack of hydrophobicity and amphipathicity...
  8. pmc Structure, topology, and tilt of cell-signaling peptides containing nuclear localization sequences in membrane bilayers determined by solid-state NMR and molecular dynamics simulation studies
    Ayyalusamy Ramamoorthy
    Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    Biochemistry 46:965-75. 2007
    ..These results and simulations on the hydrophobic fragment of SA or SKP suggest that the tilt of helices increases with a decrease in bilayer thickness without changing the phase, order, and structure of the lipid bilayers...
  9. pmc Cholesterol reduces pardaxin's dynamics-a barrel-stave mechanism of membrane disruption investigated by solid-state NMR
    Ayyalusamy Ramamoorthy
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 1055, USA
    Biochim Biophys Acta 1798:223-7. 2010
    ..These results explain the role of cholesterol in the selectivity of the broad-spectrum of antimicrobial activities of pardaxin...
  10. pmc Beyond NMR spectra of antimicrobial peptides: dynamical images at atomic resolution and functional insights
    Ayyalusamy Ramamoorthy
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    Solid State Nucl Magn Reson 35:201-7. 2009
    ....
  11. ncbi request reprint Cell selectivity correlates with membrane-specific interactions: a case study on the antimicrobial peptide G15 derived from granulysin
    Ayyalusamy Ramamoorthy
    Department of Chemistry and Biophysics Research Division, University of Michigan, 930 N University Avenue, Ann Arbor, MI 48109 1055, USA
    Biochim Biophys Acta 1758:154-63. 2006
    ..These results explain the susceptibility of E. coli and the resistance of human Jurkat cells to G15, and may have implications in designing membrane-selective therapeutic agents...
  12. pmc Antimicrobial and membrane disrupting activities of a peptide derived from the human cathelicidin antimicrobial peptide LL37
    Sathiah Thennarasu
    Department of Biophysics, University of Michigan, Ann Arbor, Michigan, USA
    Biophys J 98:248-57. 2010
    ..These results explain the susceptibility of bacteria and the resistance of erythrocytes to LL7-27, and may have implications for the design of membrane-selective therapeutic agents...
  13. pmc A model of the membrane-bound cytochrome b5-cytochrome P450 complex from NMR and mutagenesis data
    Shivani Ahuja
    Department of Chemistry and Biophysics, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Biol Chem 288:22080-95. 2013
    ..We have also shown that the addition of a substrate to cytP450 likely strengthens the cytb5-cytP450 interaction. This study paves the way to obtaining valuable structural, functional, and dynamic information on membrane-bound complexes. ..
  14. pmc Insights into antiamyloidogenic properties of the green tea extract (-)-epigallocatechin-3-gallate toward metal-associated amyloid-β species
    Suk Joon Hyung
    Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA
    Proc Natl Acad Sci U S A 110:3743-8. 2013
    ..Thus, we demonstrate the distinct antiamyloidogenic reactivity of EGCG toward metal-Aβ species with a structure-based mechanism...
  15. pmc Role of zinc in human islet amyloid polypeptide aggregation
    Jeffrey R Brender
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Am Chem Soc 132:8973-83. 2010
    ....
  16. pmc Structures of rat and human islet amyloid polypeptide IAPP(1-19) in micelles by NMR spectroscopy
    Ravi Prakash Reddy Nanga
    Department of Biophysics and Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    Biochemistry 47:12689-97. 2008
    ....
  17. pmc Delineating metabolic signatures of head and neck squamous cell carcinoma: phospholipase A2, a potential therapeutic target
    Pratima Tripathi
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, MI, United States
    Int J Biochem Cell Biol 44:1852-61. 2012
    ..Moreover, cPLA(2) may serve as a potential therapeutic target for anti-cancer therapy of HNSCC...
  18. pmc Helical conformation of the SEVI precursor peptide PAP248-286, a dramatic enhancer of HIV infectivity, promotes lipid aggregation and fusion
    Jeffrey R Brender
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan, USA
    Biophys J 97:2474-83. 2009
    ..This suggests that nonspecific binding of monomeric or small oligomeric forms of SEVI in a helical conformation to lipid membranes may be an additional mechanism by which SEVI enhances the infectivity of the HIV virus...
  19. pmc Proton-evolved local-field solid-state NMR studies of cytochrome b5 embedded in bicelles, revealing both structural and dynamical information
    Ronald Soong
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Am Chem Soc 132:5779-88. 2010
    ..Our results suggest that the PELF-based experimental approaches will have a profound impact on solid-state NMR spectroscopy of membrane proteins and other membrane-associated molecules in magnetically aligned bicelles...
  20. pmc The amyloidogenic SEVI precursor, PAP248-286, is highly unfolded in solution despite an underlying helical tendency
    Jeffrey R Brender
    Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 1055, USA
    Biochim Biophys Acta 1808:1161-9. 2011
    ..This is another noticeable difference between PAP(248-286) and other amyloid peptides, which generally show penetration into at least the headgroup region of the bilayer, and may explain some of the unusual properties of SEVI...
  21. pmc High-resolution structural insights into bone: a solid-state NMR relaxation study utilizing paramagnetic doping
    Kamal H Mroué
    Biophysics, The University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Phys Chem B 116:11656-61. 2012
    ....
  22. pmc Lipid composition-dependent membrane fragmentation and pore-forming mechanisms of membrane disruption by pexiganan (MSI-78)
    Dong Kuk Lee
    Departments of Biophysics and Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, United States
    Biochemistry 52:3254-63. 2013
    ....
  23. pmc Development of bifunctional stilbene derivatives for targeting and modulating metal-amyloid-β species
    Joseph J Braymer
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA
    Inorg Chem 50:10724-34. 2011
    ....
  24. pmc High-resolution 2D NMR spectroscopy of bicelles to measure the membrane interaction of ligands
    Sergey V Dvinskikh
    Biophysics Research Division and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Am Chem Soc 129:794-802. 2007
    ..As a first application, these solid-state NMR approaches were utilized to probe the membrane interaction of an antidepressant molecule, desipramine, and its location in the membrane...
  25. pmc Determining the effects of lipophilic drugs on membrane structure by solid-state NMR spectroscopy: the case of the antioxidant curcumin
    Jeffrey Barry
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Am Chem Soc 131:4490-8. 2009
    ..The experiments outlined here show promise for understanding the action of other drugs such as capsaicin in which drug-induced alterations of membrane structure have strong pharmacological effects...
  26. pmc Cytochrome-P450-cytochrome-b5 interaction in a membrane environment changes 15N chemical shift anisotropy tensors
    Manoj Kumar Pandey
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, United States
    J Phys Chem B 117:13851-60. 2013
    ..This may be attributed to the paramagnetic effect from Fe(III) of the heme in cytb5, which is similar to our previously reported values for the free cytb5. ..
  27. pmc Membrane fragmentation by an amyloidogenic fragment of human Islet Amyloid Polypeptide detected by solid-state NMR spectroscopy of membrane nanotubes
    Jeffrey R Brender
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 1055, USA
    Biochim Biophys Acta 1768:2026-9. 2007
    ..The disappearance of membrane fragmentation at higher concentrations of hIAPP suggests an alternate kinetic pathway to fibril formation in which membrane fragmentation is inhibited...
  28. pmc Cations as switches of amyloid-mediated membrane disruption mechanisms: calcium and IAPP
    Michele F M Sciacca
    Biophysics, University of Michigan, Ann Arbor, Michigan, USA
    Biophys J 104:173-84. 2013
    ....
  29. pmc Resolution of oligomeric species during the aggregation of Aβ1-40 using (19)F NMR
    Yuta Suzuki
    Department of Chemistry, Materials Science and Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109, United States
    Biochemistry 52:1903-12. 2013
    ..The ability to resolve individual oligomers and track their formation in real-time should prove fruitful in understanding the aggregation of amyloidogenic proteins and in isolating potentially toxic nonamyloid oligomers...
  30. pmc Site specific interaction of the polyphenol EGCG with the SEVI amyloid precursor peptide PAP(248-286)
    Nataliya Popovych
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Phys Chem B 116:3650-8. 2012
    ..The results of this study could aid in the development of small molecule inhibitors of SEVI and other amyloid proteins...
  31. pmc Fast NMR data acquisition from bicelles containing a membrane-associated peptide at natural-abundance
    Kazutoshi Yamamoto
    Biophysics and Department of Chemistry, The University of Michigan, Ann Arbor, Michigan 48109 1055, United States
    J Phys Chem B 115:12448-55. 2011
    ..These results demonstrate that this approach will be useful in the structural studies of membrane-associated peptides and proteins without the need for isotopic enrichment for solution NMR studies...
  32. pmc Natural-abundance 43Ca solid-state NMR spectroscopy of bone
    Jiadi Xu
    Biophysics, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Am Chem Soc 132:11504-9. 2010
    ....
  33. pmc Biphasic effects of insulin on islet amyloid polypeptide membrane disruption
    Jeffrey R Brender
    Department of Biophysics, University of Michigan, Ann Arbor, Michigan, USA
    Biophys J 100:685-92. 2011
    ..These results suggest that insulin has a more complicated role in inhibiting IAPP fibrillogenesis, and that other factors, such as the low pH of the secretory granule, may also play a role...
  34. pmc Solid-state NMR reveals the hydrophobic-core location of poly(amidoamine) dendrimers in biomembranes
    Pieter E S Smith
    Biophysics, University of Michigan, Ann Arbor, Michigan, USA
    J Am Chem Soc 132:8087-97. 2010
    ....
  35. pmc Limiting an antimicrobial peptide to the lipid-water interface enhances its bacterial membrane selectivity: a case study of MSI-367
    Sathiah Thennarasu
    Department of Biophysics, University of Michigan, Ann Arbor, Michigan 48109 1055, United States
    Biochemistry 49:10595-605. 2010
    ....
  36. pmc Role of cationic group structure in membrane binding and disruption by amphiphilic copolymers
    Edmund F Palermo
    Macromolecular Science and Engineering Center, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Phys Chem B 115:366-75. 2011
    ..This is likely due to enhanced complexation of primary amines to the phosphate groups in the lipids, through a combination of hydrogen bonding and electrostatic interactions...
  37. doi request reprint Absorbance-based assay for membrane disruption by antimicrobial peptides and synthetic copolymers using pyrroloquinoline quinone-loaded liposomes
    Laura B Zimmerman
    Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA
    Anal Biochem 411:194-9. 2011
    ..The assay is simple and sensitive and will be useful for analyzing the membrane permeation/disruption properties of a host of antimicrobial peptides and synthetic polymers...
  38. pmc Membrane thinning due to antimicrobial peptide binding: an atomic force microscopy study of MSI-78 in lipid bilayers
    Almut Mecke
    University of Michigan, Ann Arbor, Michigan, USA
    Biophys J 89:4043-50. 2005
    ..Furthermore, 2H NMR experiments on DMPC-d54 multilamellar vesicles indicate that the acyl chains of DMPC are highly disordered in the presence of the peptide as is to be expected for the proposed structure of the peptide-lipid assembly...
  39. ncbi request reprint Membrane permeabilization, orientation, and antimicrobial mechanism of subtilosin A
    Sathiah Thennarasu
    Department of Chemistry and Biophysics Research Division, University of Michigan, Ann Arbor, MI 48109 1055, USA
    Chem Phys Lipids 137:38-51. 2005
    ..These results suggest that the lipid perturbation observed in this study may be one of the consequences of subtilosin A binding to lipid bilayers, which results in membrane permeabilization at high peptide concentrations...
  40. pmc Induction of negative curvature as a mechanism of cell toxicity by amyloidogenic peptides: the case of islet amyloid polypeptide
    Pieter E S Smith
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Am Chem Soc 131:4470-8. 2009
    ....
  41. pmc Bicelle-enabled structural studies on a membrane-associated cytochrome B5 by solid-state MAS NMR spectroscopy
    Jiadi Xu
    Biophysics and Department of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, MI 48109 1055, USA
    Angew Chem Int Ed Engl 47:7864-7. 2008
  42. pmc Antimicrobial activity and membrane selective interactions of a synthetic lipopeptide MSI-843
    Sathiah Thennarasu
    Biophysics Research Division and Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 1055, USA
    Biochim Biophys Acta 1711:49-58. 2005
    ..Combination of all these data suggests the formation of a lipid-peptide complex resulting in a transient pore as a plausible mechanism for the membrane permeabilization and antimicrobial activity of the lipopeptide MSI-843...
  43. pmc Sensitivity and resolution enhancement in solid-state NMR spectroscopy of bicelles
    Sergey V Dvinskikh
    Biophysics Research Division and Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109 1055, USA
    J Magn Reson 184:228-35. 2007
    ..The possibility of using (14)N experiments on bicelles is also discussed...
  44. pmc Phosphatidylethanolamine enhances amyloid fiber-dependent membrane fragmentation
    Michele F M Sciacca
    Departments of Biophysics and Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, United States
    Biochemistry 51:7676-84. 2012
    ..These findings provide insights into the mechanism of membrane disruption induced by IAPP, suggesting a possible role of PE and other amyloids involved in other pathologies...
  45. ncbi request reprint Solid-state NMR and molecular dynamics simulations reveal the oligomeric ion-channels of TM2-GABA(A) stabilized by intermolecular hydrogen bonding
    Senthil K Kandasamy
    Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109 1055, USA
    Biochim Biophys Acta 1788:686-95. 2009
    ..The results also suggest that peptide-peptide interactions in the tilted transmembrane orientation overcome the hydrophobic mismatch between the peptide and bilayer thickness...
  46. pmc Use of a copper-chelated lipid speeds up NMR measurements from membrane proteins
    Kazutoshi Yamamoto
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Am Chem Soc 132:6929-31. 2010
    ..Furthermore, solid-state NMR measurements are shown to be feasible even for nanomole concentrations of a membrane-associated peptide...
  47. ncbi request reprint Structures of the dimeric and monomeric variants of magainin antimicrobial peptides (MSI-78 and MSI-594) in micelles and bilayers, determined by NMR spectroscopy
    Fernando Porcelli
    Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Biochemistry 45:5793-9. 2006
    ..Since magainin activity is modulated by oligomerization within the membrane bilayers, this study represents a step forward in understanding the role of self-association in determining magainin function...
  48. pmc Structure and orientation of pardaxin determined by NMR experiments in model membranes
    Fernando Porcelli
    Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 279:45815-23. 2004
    ..These new data help explain how the non-covalent interactions of Pa4 with lipid membranes induce a stable secondary structure and provide an atomic view of the membrane insertion process of Pa4...
  49. pmc A single mutation in the nonamyloidogenic region of islet amyloid polypeptide greatly reduces toxicity
    Jeffrey R Brender
    Department of Chemistry and Biophysics, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    Biochemistry 47:12680-8. 2008
    ..Our results indicate that the modulation of the peptide orientation in the membrane by His18 plays a key role in the toxicity of nonamyloidogenic forms of hIAPP...
  50. ncbi request reprint Using fluorous amino acids to probe the effects of changing hydrophobicity on the physical and biological properties of the beta-hairpin antimicrobial peptide protegrin-1
    Lindsey M Gottler
    Department of Chemistry, Dental School, University of Michigan, Ann Arbor, Michigan 48109, USA
    Biochemistry 47:9243-50. 2008
    ....
  51. pmc Dynamic interaction between membrane-bound full-length cytochrome P450 and cytochrome b5 observed by solid-state NMR spectroscopy
    Kazutoshi Yamamoto
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 1055
    Sci Rep 3:2538. 2013
    ....
  52. pmc Membrane disordering is not sufficient for membrane permeabilization by islet amyloid polypeptide: studies of IAPP(20-29) fragments
    Jeffrey R Brender
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 1055, USA
    Phys Chem Chem Phys 15:8908-15. 2013
    ....
  53. doi request reprint Three-dimensional structure and orientation of rat islet amyloid polypeptide protein in a membrane environment by solution NMR spectroscopy
    Ravi Prakash Reddy Nanga
    Department of Chemistry and Biophysics, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Am Chem Soc 131:8252-61. 2009
    ....
  54. pmc Two-step mechanism of membrane disruption by Aβ through membrane fragmentation and pore formation
    Michele F M Sciacca
    Biophysics, University of Michigan, Ann Arbor, Michigan, USA
    Biophys J 103:702-10. 2012
    ..These results provide insights into the toxicity of Aβ and may aid in the design of specific compounds to alleviate the neurodegeneration of Alzheimer's disease...
  55. pmc Alternative pathways of human islet amyloid polypeptide aggregation distinguished by (19)f nuclear magnetic resonance-detected kinetics of monomer consumption
    Yuta Suzuki
    Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA
    Biochemistry 51:8154-62. 2012
    ..By comparing the rates of monomer consumption and fiber formation, we are able to show that EGCG stabilizes nonfibrillar large aggregates during fibrillogenesis...
  56. pmc Does cholesterol suppress the antimicrobial peptide induced disruption of lipid raft containing membranes?
    Austin J McHenry
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    Biochim Biophys Acta 1818:3019-24. 2012
    ..Our results show that the peptide selectivity is not only dependent on the lipid phase but also on the presence of phase separation in heterogeneous lipid systems...
  57. pmc A two-site mechanism for the inhibition of IAPP amyloidogenesis by zinc
    Samer Salamekh
    Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 1055, USA
    J Mol Biol 410:294-306. 2011
    ..Combined, these results suggest that zinc promotes the formation of oligomers while creating an energetic barrier for the formation of amyloid fibers...
  58. pmc Cross-correlations between low-γ nuclei in solids via a common dipolar bath
    Aanatoly K Khitrin
    Biophysics and Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109 1055, USA
    J Magn Reson 212:95-101. 2011
    ..In addition to the thermodynamic analysis of mixing pulse sequences, two different new mixing sequences utilizing adiabatic pulses are also experimentally demonstrated...
  59. pmc Probing the spontaneous membrane insertion of a tail-anchored membrane protein by sum frequency generation spectroscopy
    Khoi Tan Nguyen
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Am Chem Soc 132:15112-5. 2010
    ..The approach reported in this study could be used as a potential rapid screening tool in measuring the topology of membrane proteins as well as interactions of biomolecules with lipid bilayers in situ...
  60. pmc INEPT-based separated-local-field NMR spectroscopy: a unique approach to elucidate side-chain dynamics of membrane-associated proteins
    Jiadi Xu
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Am Chem Soc 132:9944-7. 2010
    ..Therefore, using an appropriate model, it is possible to determine side-chain dynamics and provide additional information on the topology and function of a membrane protein in its native environment...
  61. pmc NMR structure in a membrane environment reveals putative amyloidogenic regions of the SEVI precursor peptide PAP(248-286)
    Ravi P R Nanga
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Am Chem Soc 131:17972-9. 2009
    ..The structure presented here can be used for the rational design of mutagenesis studies on SEVI amyloid formation and viral infection enhancement...
  62. pmc MetaboID: a graphical user interface package for assignment of 1H NMR spectra of bodyfluids and tissues
    Neil MacKinnon
    Biophysics, University of Michigan, Ann Arbor, MI 48109 1055, USA
    J Magn Reson 226:93-9. 2013
    ..Such a toolbox is expected to be attractive to both experienced and new metabolomic researchers as well as general complex mixture analysts...
  63. pmc Determination of 15N chemical shift anisotropy from a membrane-bound protein by NMR spectroscopy
    Manoj Kumar Pandey
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Phys Chem B 116:7181-9. 2012
    ..We believe that the CSA parameters reported in this study will be useful in determining the structure, dynamics, and orientation of proteins, including membrane proteins, using NMR spectroscopy...
  64. pmc Design of small molecules that target metal-A{beta} species and regulate metal-induced A{beta} aggregation and neurotoxicity
    Jung Suk Choi
    Department of Chemistry, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA
    Proc Natl Acad Sci U S A 107:21990-5. 2010
    ....
  65. doi request reprint Using fluorous amino acids to modulate the biological activity of an antimicrobial peptide
    Lindsey M Gottler
    Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA
    Chembiochem 9:370-3. 2008
  66. doi request reprint Association of highly compact type II diabetes related islet amyloid polypeptide intermediate species at physiological temperature revealed by diffusion NMR spectroscopy
    Ronald Soong
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Am Chem Soc 131:7079-85. 2009
    ....
  67. ncbi request reprint Investigation of the interaction of myelin basic protein with phospholipid bilayers using solid-state NMR spectroscopy
    Caroline D Pointer-Keenan
    Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 1055, USA
    Chem Phys Lipids 132:47-54. 2004
    ..These results demonstrate that the MBP-induced fragmentation of POPC bilayers is due to the freeze-thaw cycles used in the preparation of multilamellar vesicles and not due to intrinsic protein-lipid interactions...
  68. pmc A proton spin diffusion based solid-state NMR approach for structural studies on aligned samples
    Jiadi Xu
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, United States
    J Phys Chem B 115:4863-71. 2011
    ..We expect this technique to also be valuable in the structural studies of functional molecules like columnar liquid crystals and other biomaterials...
  69. pmc Variable reference alignment: an improved peak alignment protocol for NMR spectral data with large intersample variation
    Neil MacKinnon
    Biophysics, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    Anal Chem 84:5372-9. 2012
    ....
  70. pmc Misfolded proteins in Alzheimer's disease and type II diabetes
    Alaina S Detoma
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA
    Chem Soc Rev 41:608-21. 2012
    ..Small molecules that target and modulate the metal-Aβ interaction and neurotoxicity are included to illustrate one of the current approaches for uncovering the complexities of protein misfolding at the molecular level...
  71. pmc A high-resolution solid-state NMR approach for the structural studies of bicelles
    Sergey Dvinskikh
    Biophysics Research Division and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Am Chem Soc 128:6326-7. 2006
    ....
  72. doi request reprint Amyloid fiber formation and membrane disruption are separate processes localized in two distinct regions of IAPP, the type-2-diabetes-related peptide
    Jeffrey R Brender
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Am Chem Soc 130:6424-9. 2008
    ..These results indicate that membrane disruption can occur independently from amyloid formation in IAPP, and the sequences responsible for amyloid formation and membrane disruption are located in different regions of the peptide...
  73. pmc Solid-state NMR reveals structural and dynamical properties of a membrane-anchored electron-carrier protein, cytochrome b5
    Ulrich H N Dürr
    Biophysics Research Division and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Am Chem Soc 129:6670-1. 2007
    ..Two-dimensional 1H-15N HIMSELF spectra showed PISA-wheel patterns reporting on the structure and dynamics of the membrane anchor of the protein...
  74. pmc Solid-state NMR spectroscopy provides atomic-level insights into the dehydration of cartilage
    Jiadi Xu
    Biophysics, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Phys Chem B 115:9948-54. 2011
    ..Equally interestingly, our results demonstrate that the dehydration effects are reversible, and the molecular structure and mobility are restored upon rehydration...
  75. pmc A partially folded structure of amyloid-beta(1-40) in an aqueous environment
    Subramanian Vivekanandan
    Department of Biophysics, University of Michigan, Ann Arbor, MI 48109 1055, USA
    Biochem Biophys Res Commun 411:312-6. 2011
    ..Helical intermediates have been predicted to be crucial on-pathway intermediates in amyloid fibrillogenesis, and the structure presented here presents a new target for investigation of early events in Aβ(1-40) fibrillogenesis...
  76. pmc Structure and membrane orientation of IAPP in its natively amidated form at physiological pH in a membrane environment
    Ravi Prakash Reddy Nanga
    Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 1055, USA
    Biochim Biophys Acta 1808:2337-42. 2011
    ..The greater helical content of human IAPP in the amidated versus free acid form is likely to play a role in its aggregation and membrane disruptive activity...
  77. pmc Structure, membrane orientation, mechanism, and function of pexiganan--a highly potent antimicrobial peptide designed from magainin
    Lindsey M Gottler
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 1055, USA
    Biochim Biophys Acta 1788:1680-6. 2009
    ..This article covers functional, biophysical, biochemical and structural studies on pexiganan...
  78. pmc Membrane orientation of MSI-78 measured by sum frequency generation vibrational spectroscopy
    Pei Yang
    Biophysics and Department of Chemistry, 930 North University Avenue, University of Michigan, Ann Arbor, Michigan 48109, USA
    Langmuir 27:7760-7. 2011
    ..The deduced lipid flip-flop from SFG signals observed from lipids also supports MSI-78-induced toroidal-type pore formation...
  79. pmc An innovative procedure using a sublimable solid to align lipid bilayers for solid-state NMR studies
    Kevin J Hallock
    Department of Chemistry, The University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    Biophys J 82:2499-503. 2002
    ..The increase in bilayer alignment implies that this procedure will improve the sensitivity of solid-state NMR experiments, in particular those techniques that detect low-sensitivity nuclei, such as 15N and 13C...
  80. ncbi request reprint The spectrum of antimicrobial activity of the bacteriocin subtilosin A
    Charles E Shelburne
    Department of Biologic and Materials Sciences, The University of Michigan School of Dentistry, 1210 Eisenhower Place, Ann Arbor, MI 48108, USA
    J Antimicrob Chemother 59:297-300. 2007
    ..Subtilosin A is a bacteriocin produced by Bacillus subtilis that has activity against Listeria monocytogenes, which might indicate antimicrobial activity unusual for bacteriocins...
  81. doi request reprint Fluorine--a new element in the design of membrane-active peptides
    E Neil G Marsh
    Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 1055, USA
    Mol Biosyst 5:1143-7. 2009
    ..In this review article, we present the use of fluorinated amino acids in the design of antimicrobial peptides and other membrane-active peptides...
  82. ncbi request reprint Effects of antidepressants on the conformation of phospholipid headgroups studied by solid-state NMR
    Jose S Santos
    Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    Magn Reson Chem 42:105-14. 2004
    ..The use of (14)N as a probe was instrumental in arriving at the presented conclusions...
  83. pmc NMR characterization of monomeric and oligomeric conformations of human calcitonin and its interaction with EGCG
    Rui Huang
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 1055, USA
    J Mol Biol 416:108-20. 2012
    ..The NMR experiments also indicate that the interaction between aromatic rings of EGCG and the aromatic side chains of the peptide may play an important role in inhibiting fibril formation of hCT...
  84. ncbi request reprint The cytochromes P450 and b5 and their reductases--promising targets for structural studies by advanced solid-state NMR spectroscopy
    Ulrich H N Dürr
    Biophysics and Department of Chemistry, University of Michigan, 930 N University Avenue, Ann Arbor, MI 48109 1055, USA
    Biochim Biophys Acta 1768:3235-59. 2007
    ..We aim to give a detailed account of modern techniques as applicable to cyt P450 and cyt b5, to show what has already been possible and what seems to be viable in the very near future...
  85. ncbi request reprint Heteronuclear isotropic mixing separated local field NMR spectroscopy
    Sergey V Dvinskikh
    Biophysics Research Division, University of Michigan, Ann Arbor, MI 48109 1055, USA
    J Chem Phys 125:34507. 2006
    ....
  86. pmc Comprehensive analysis of lipid dynamics variation with lipid composition and hydration of bicelles using nuclear magnetic resonance (NMR) spectroscopy
    Kazutoshi Yamamoto
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    Langmuir 25:7010-8. 2009
    ....
  87. ncbi request reprint Solid-state NMR spectroscopy of aligned lipid bilayers at low temperatures
    Dong Kuk Lee
    Department of Chemistry, Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Am Chem Soc 126:2318-9. 2004
    ..The increased lifetime of the alignment and the nature of the bilayer sample would enhance the application of solid-state NMR techniques to study membrane proteins...
  88. pmc Polymorphs and hydrates of acyclovir
    Katie M Lutker
    Department of Chemistry, Biophysics, and the Macromolecular Science and Engineering Program, The University of Michigan, 930 North University, Ann Arbor, Michigan 48109 1055, USA
    J Pharm Sci 100:949-63. 2011
    ..The high-temperature modification is achieved by heating anhydrous form I above 180 °C. The crystal structures of anhydrous form I and a novel hydrate are reported for the first time...
  89. ncbi request reprint The human beta-defensin-3, an antibacterial peptide with multiple biological functions
    Vishnu Dhople
    Biophysics Research Division and Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109 1055, USA
    Biochim Biophys Acta 1758:1499-512. 2006
    ..The future scope for studies on HbetaD-3 and design of short potent antimicrobial peptides, based on the native HbetaD-3 molecule, that do not interfere in the immunomodulatory function is also outlined...
  90. ncbi request reprint A study of a Calpha,beta-didehydroalanine homo-oligopeptide series in the solid-state by 13C cross-polarization magic angle spinning NMR
    Katherine A Henzler Wildman
    Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 1055, USA
    J Pept Sci 10:336-41. 2004
    ..The compounds examined are members of a terminally protected, homo-oligopeptide series (from monomer through hexamer) based on Calpha,beta-didehydroalanine...
  91. doi request reprint High-resolution characterization of liquid-crystalline [60]fullerenes using solid-state nuclear magnetic resonance spectroscopy
    Sergey V Dvinskikh
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 1055, USA
    J Phys Chem B 112:12347-53. 2008
    ..Our NMR results suggest that the fullerene-ferrocene dyads form highly dynamic liquid-crystalline phases in which molecules rotate fast around the symmetry axis on the characteristic NMR time scale of approximately 10 (-4) s...
  92. pmc Synthetic and natural polycationic polymer nanoparticles interact selectively with fluid-phase domains of DMPC lipid bilayers
    Almut Mecke
    Department of Physics, The University of Michigan, Ann Arbor, Michigan 48109, USA
    Langmuir 21:8588-90. 2005
    ..We find that regions of the bilayer that are in the gel phase are unaffected by the presence of polymers, whereas the liquid phase is disrupted...
  93. ncbi request reprint NMR structural studies on membrane proteins
    Ayyalusamy Ramamoorthy
    University of Michigan, Ann Arbor, MI 48109 1055, USA
    Biochim Biophys Acta 1768:2947-8. 2007
  94. ncbi request reprint LL-37, the only human member of the cathelicidin family of antimicrobial peptides
    Ulrich H N Dürr
    Biophysics Research Division and Department of Chemistry, 930 N University Avenue, University of Michigan, Ann Arbor, MI 48109 1055, USA
    Biochim Biophys Acta 1758:1408-25. 2006
    ..The multitude of immuno-functional roles is only outlined. We believe that this review will aid the future work on the biophysical, biochemical and immunological investigations of this highly intriguing molecule...
  95. ncbi request reprint A 2D solid-state NMR experiment to resolve overlapping aromatic resonances of thiophene-based nematogens
    Tanneru Narasimhaswamy
    Biophysics Research Division and Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 1055, USA
    J Am Chem Soc 127:6958-9. 2005
    ..We believe that the 2D solid-state NMR can be extended to other types of liquid crystalline materials such as the banana-based mesogens for determining the orientational order and bent angle...
  96. doi request reprint Two-dimensional homonuclear chemical shift correlation established by the cross-relaxation driven spin diffusion in solids
    Jiadi Xu
    Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    J Chem Phys 128:052308. 2008
    ..The speedy spin diffusion process rendered by the CRDSD approach will be useful to assign resonances from a uniformly (15)N or (13)C labeled proteins and peptides, particularly in aligned samples...
  97. ncbi request reprint Characterization of metal centers in bioinorganic complexes using ab initio calculations of 113Cd chemical shifts
    Srikanth S Kidambi
    Biophysics Research Division, Department of Chemistry, and Macromolecular Science and Engineering, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    Inorg Chem 42:2200-2. 2003
    ..Our results infer that the density functional theory using the Sadlej basis set on the cadmium atom is a suitable method for estimating cadmium shielding values to a reasonable accuracy...
  98. pmc Time-resolved dehydration-induced structural changes in an intact bovine cortical bone revealed by solid-state NMR spectroscopy
    Peizhi Zhu
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Am Chem Soc 131:17064-5. 2009
    ..These results provide insights into the role of water molecules in the bone structure and shed light on the relationship between the structure and mechanics of bone...
  99. pmc Small molecule modulators of copper-induced Abeta aggregation
    Sarmad S Hindo
    Department of Chemistry and the Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Am Chem Soc 131:16663-5. 2009
    ..These studies suggest a new class of multifunctional molecules for the development of chemical tools to unravel metal-associated events in AD and potential therapeutic agents for metal-ion chelation therapy...

Research Grants10

  1. Structure and function of antimicrobial peptides
    Ayyalusamy Ramamoorthy; Fiscal Year: 2004
    ..These peptides have therapeutic potential as antibiotics and have particular importance for cystic fibrosis patients. ..
  2. Structural Studies on Membrane-Associate Cytochrome B5 and P450 NMR
    Ayyalusamy Ramamoorthy; Fiscal Year: 2011
    ..These studies will also enable us to understand the role of cytochrome-b5 in the biosynthesis of testosterone and numerous unsaturated lipids, which are necessary for maintaining the integrity of cellular membranes. ..
  3. Structural Studies on Membrane-Associate Cytochrome B5 and P450 NMR
    Ayyalusamy Ramamoorthy; Fiscal Year: 2010
    ..These studies will also enable us to understand the role of cytochrome-b5 in the biosynthesis of testosterone and numerous unsaturated lipids, which are necessary for maintaining the integrity of cellular membranes. ..
  4. Structural Studies on Membrane-Associate Cytochrome B5 and P450 NMR
    Ayyalusamy Ramamoorthy; Fiscal Year: 2009
    ..These studies will also enable us to understand the role of cytochrome-b5 in the biosynthesis of testosterone and numerous unsaturated lipids, which are necessary for maintaining the integrity of cellular membranes. ..
  5. Structure and function of antimicrobial peptides
    Ayyalusamy Ramamoorthy; Fiscal Year: 2007
    ..These peptides have therapeutic potential as antibiotics and have particular importance for cystic fibrosis patients. ..
  6. Purchase of a 500 MHz Solid State NMR Spectrometer
    Ayyalusamy Ramamoorthy; Fiscal Year: 2007
    ..All the major users of the instrument are supported by NIH R01 grants. ..
  7. Structure and function of antimicrobial peptides
    Ayyalusamy Ramamoorthy; Fiscal Year: 2006
    ..These peptides have therapeutic potential as antibiotics and have particular importance for cystic fibrosis patients. ..
  8. Structure and function of antimicrobial peptides
    Ayyalusamy Ramamoorthy; Fiscal Year: 2005
    ..These peptides have therapeutic potential as antibiotics and have particular importance for cystic fibrosis patients. ..
  9. Rapid and efficient production of functional Cytochrome P450 enzymatic complex fo
    Ayyalusamy Ramamoorthy; Fiscal Year: 2010
    ....