Miguel Ramalho-Santos

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc High-efficiency stem cell fusion-mediated assay reveals Sall4 as an enhancer of reprogramming
    Connie C Wong
    Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 3:e1955. 2008
  2. doi request reprint On the origin of the term "stem cell"
    Miguel Ramalho-Santos
    Institute for Regeneration Medicine, University of California, San Francisco, San Francisco, CA 94143, USA
    Cell Stem Cell 1:35-8. 2007
  3. pmc iPS cells: insights into basic biology
    Miguel Ramalho-Santos
    Department of Ob Gyn, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA 94143 0525, USA
    Cell 138:616-8. 2009
  4. pmc Transcriptional analysis of pluripotency reveals the Hippo pathway as a barrier to reprogramming
    Han Qin
    Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, and Diabetes Center, South San Francisco, CA, USA
    Hum Mol Genet 21:2054-67. 2012
  5. pmc Parallel gateways to pluripotency: open chromatin in stem cells and development
    Fong Ming Koh
    Departments of Ob Gyn and Pathology, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences and Diabetes Center, University of California, San Francisco, San Francisco, CA 94143 0525, USA
    Curr Opin Genet Dev 20:492-9. 2010
  6. pmc Bivalent chromatin marks developmental regulatory genes in the mouse embryonic germline in vivo
    Michael Sachs
    Biomedical Sciences Graduate Program, 35 Medical Center Way, University of California, San Francisco, CA 94143, USA
    Cell Rep 3:1777-84. 2013
  7. pmc Open chromatin in pluripotency and reprogramming
    Alexandre Gaspar-Maia
    Departments of Ob Gyn and Pathology, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences and Diabetes Center, University of California, San Francisco, 513 Parnassus Ave, San Francisco, California 94143 0525, USA
    Nat Rev Mol Cell Biol 12:36-47. 2011
  8. pmc Systematic identification of cis-regulatory sequences active in mouse and human embryonic stem cells
    Marica Grskovic
    Institute for Regeneration Medicine, University of California San Francisco, San Francisco, California, United States of America
    PLoS Genet 3:e145. 2007
  9. ncbi request reprint Somatic cells regulate maternal mRNA translation and developmental competence of mouse oocytes
    Jing Chen
    1 Center for Reproductive Sciences, University of California, San Francisco, California 94143, USA 2 Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, California 94143, USA 3 Department of Obstetrics and Gynecology and Reproductive Sciences, University of California, San Francisco, California 94143, USA
    Nat Cell Biol 15:1415-23. 2013
  10. pmc Polycomb-like 3 promotes polycomb repressive complex 2 binding to CpG islands and embryonic stem cell self-renewal
    Julie Hunkapiller
    Department of Biochemistry and Biophysics, Cardiovascular Research Institute, University of California San Francisco, San Francisco, California, USA
    PLoS Genet 8:e1002576. 2012

Collaborators

Detail Information

Publications18

  1. pmc High-efficiency stem cell fusion-mediated assay reveals Sall4 as an enhancer of reprogramming
    Connie C Wong
    Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 3:e1955. 2008
    ..These results indicate that improvements in reprogramming assays, including fusion assays, may allow the systematic identification and molecular characterization of enhancers of somatic cell reprogramming...
  2. doi request reprint On the origin of the term "stem cell"
    Miguel Ramalho-Santos
    Institute for Regeneration Medicine, University of California, San Francisco, San Francisco, CA 94143, USA
    Cell Stem Cell 1:35-8. 2007
    ..The original meanings of the term stem cell, rather than being historical relics, continue to capture important aspects of the biology of stem cells as we see them today...
  3. pmc iPS cells: insights into basic biology
    Miguel Ramalho-Santos
    Department of Ob Gyn, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA 94143 0525, USA
    Cell 138:616-8. 2009
    ..The discovery that adult somatic cells can be induced to become pluripotent by overexpression of a few key transcription factors provides an exciting new window into the basic biology of pluripotency and differentiation...
  4. pmc Transcriptional analysis of pluripotency reveals the Hippo pathway as a barrier to reprogramming
    Han Qin
    Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, and Diabetes Center, South San Francisco, CA, USA
    Hum Mol Genet 21:2054-67. 2012
    ..These results reveal transcriptional parallels between germ cell transformation and the generation of iPS cells and indicate that the Hippo pathway constitutes a barrier to cellular reprogramming...
  5. pmc Parallel gateways to pluripotency: open chromatin in stem cells and development
    Fong Ming Koh
    Departments of Ob Gyn and Pathology, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences and Diabetes Center, University of California, San Francisco, San Francisco, CA 94143 0525, USA
    Curr Opin Genet Dev 20:492-9. 2010
    ..The combination of in vitro and in vivo approaches is expected to provide a comprehensive understanding of the epigenetic regulation of pluripotency and reprograming...
  6. pmc Bivalent chromatin marks developmental regulatory genes in the mouse embryonic germline in vivo
    Michael Sachs
    Biomedical Sciences Graduate Program, 35 Medical Center Way, University of California, San Francisco, CA 94143, USA
    Cell Rep 3:1777-84. 2013
    ..Our results demonstrate the existence of bivalent domains in the germline and raise the possibility that the somatic program is continuously maintained as bivalent, potentially imparting transgenerational epigenetic inheritance. ..
  7. pmc Open chromatin in pluripotency and reprogramming
    Alexandre Gaspar-Maia
    Departments of Ob Gyn and Pathology, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences and Diabetes Center, University of California, San Francisco, 513 Parnassus Ave, San Francisco, California 94143 0525, USA
    Nat Rev Mol Cell Biol 12:36-47. 2011
    ..These same principles may apply during reacquisition of an open chromatin state upon reprogramming to pluripotency, and during de-differentiation in cancer...
  8. pmc Systematic identification of cis-regulatory sequences active in mouse and human embryonic stem cells
    Marica Grskovic
    Institute for Regeneration Medicine, University of California San Francisco, San Francisco, California, United States of America
    PLoS Genet 3:e145. 2007
    ..Our results suggest that this systematic approach can be broadly applied to understanding transcriptional networks in mammalian species...
  9. ncbi request reprint Somatic cells regulate maternal mRNA translation and developmental competence of mouse oocytes
    Jing Chen
    1 Center for Reproductive Sciences, University of California, San Francisco, California 94143, USA 2 Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, California 94143, USA 3 Department of Obstetrics and Gynecology and Reproductive Sciences, University of California, San Francisco, California 94143, USA
    Nat Cell Biol 15:1415-23. 2013
    ..These somatic cell signals that affect translation require activation of the PI(3)K-AKT-mTOR pathway. Thus, mRNA translation depends on somatic cell cues that are essential to reprogramme the oocyte for embryo development. ..
  10. pmc Polycomb-like 3 promotes polycomb repressive complex 2 binding to CpG islands and embryonic stem cell self-renewal
    Julie Hunkapiller
    Department of Biochemistry and Biophysics, Cardiovascular Research Institute, University of California San Francisco, San Francisco, California, USA
    PLoS Genet 8:e1002576. 2012
    ..Thus, Pcl3 is a component of PRC2 critical for ESC self-renewal, histone methylation, and recruitment of PRC2 to a subset of its genomic sites...
  11. pmc Vitamin C induces Tet-dependent DNA demethylation and a blastocyst-like state in ES cells
    Kathryn Blaschke
    Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Department of Obstetrics and Gynecology and Center for Reproductive Sciences, University of California San Francisco, San Francisco, California 94143, USA
    Nature 500:222-6. 2013
    ..Collectively, the results of this study establish vitamin C as a direct regulator of Tet activity and DNA methylation fidelity in ES cells...
  12. pmc Smad2 is essential for maintenance of the human and mouse primed pluripotent stem cell state
    Masayo Sakaki-Yumoto
    Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Program in Cell Biology, University of California, San Francisco, California 94143, USA
    J Biol Chem 288:18546-60. 2013
    ..These findings reveal that Smad2 is a unique integrator of transcription and signaling events and is essential for the maintenance of the mouse and human primed pluripotent stem cell state. ..
  13. pmc Chd1 regulates open chromatin and pluripotency of embryonic stem cells
    Alexandre Gaspar-Maia
    Department of Ob Gyn and Pathology, Center for Reproductive Sciences and Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, California 94143 0525, USA
    Nature 460:863-8. 2009
    ..Our results indicate that Chd1 is essential for open chromatin and pluripotency of embryonic stem cells, and for somatic cell reprogramming to the pluripotent state...
  14. ncbi request reprint Stem cells as probabilistic self-producing entities
    Miguel Ramalho-Santos
    Program in Developmental and Stem Cell Biology, University of California, San Francisco, San Francisco CA 94143 0540, USA
    Bioessays 26:1013-6. 2004
    ..I discuss how this framework generates testable hypotheses regarding stem-cell functions...
  15. pmc Histone variant H3.3 maintains a decondensed chromatin state essential for mouse preimplantation development
    Chih Jen Lin
    Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Department of Obstetrics and Gynecology and Center for Reproductive Sciences, University of California San Francisco, 35 Medical Center Way, San Francisco, CA 94143, USA
    Development 140:3624-34. 2013
    ..These results reveal that H3.3 mediates a balance between open and condensed chromatin that is crucial for the fidelity of chromosome segregation during early mouse development. ..
  16. doi request reprint Incomplete DNA methylation underlies a transcriptional memory of somatic cells in human iPS cells
    Yuki Ohi
    Department of Ob Gyn and Center for Reproductive Sciences, University of California San Francisco, 513 Parnassus Avenue, San Francisco, California 94143, USA
    Nat Cell Biol 13:541-9. 2011
    ..Knockdown of the incompletely reprogrammed gene C9orf64 (chromosome 9 open reading frame 64) reduces the efficiency of human iPS cell generation, indicating that somatic memory genes may be functionally relevant during reprogramming...
  17. pmc CompMoby: comparative MobyDick for detection of cis-regulatory motifs
    Christina Chaivorapol
    Department of Biochemistry and Biophysics, California Institute for Quantitative Biomedical Research, Graduate Program in Biological and Medical Informatics, University of California, San Francisco, CA 94143 2540, USA
    BMC Bioinformatics 9:455. 2008
    ..Therefore, a computational algorithm should account for both conserved and nonconserved cis-regulatory elements in metazoans...
  18. ncbi request reprint "Stemness": transcriptional profiling of embryonic and adult stem cells
    Miguel Ramalho-Santos
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute HHMI, Harvard University, Cambridge, MA 02138, USA
    Science 298:597-600. 2002
    ..Embryonic and neural stem cells have many similarities at the transcriptional level. These results provide a foundation for a more detailed understanding of stem cell biology...