Alexander L Rakhmilevich

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. ncbi request reprint Treatment of experimental breast cancer using interleukin-12 gene therapy combined with anti-vascular endothelial growth factor receptor-2 antibody
    Alexander L Rakhmilevich
    K4 413 Clinical Science Center, Department of Human Oncology, University of Wisconsin Madison, 600 Highland Avenue, Madison, WI 53792, USA
    Mol Cancer Ther 3:969-76. 2004
  2. pmc Tumor-associated myeloid cells can be activated in vitro and in vivo to mediate antitumor effects
    Alexander L Rakhmilevich
    Department of Human Oncology, University of Wisconsin, 4136 WIMR, 1111 Highland Avenue, Madison, WI 53705, USA
    Cancer Immunol Immunother 61:1683-97. 2012
  3. pmc T-cell-independent antitumor effects of CD40 ligation
    Alexander L Rakhmilevich
    Department of Human Oncology and Paul P Carbone Comprehensive Cancer Center, University of Wisconsin Madison, Madison, WI 53705, USA
    Int Rev Immunol 31:267-78. 2012
  4. doi request reprint CD40 ligation in vivo can induce T cell independent antitumor effects even against immunogenic tumors
    Alexander L Rakhmilevich
    The Department of Human Oncology, University of Wisconsin, K4 413 Clinical Science Center, 600 Highland Avenue, Madison, WI 53792, USA
    Cancer Immunol Immunother 57:1151-60. 2008
  5. ncbi request reprint In vivo CD40 ligation can induce T-cell-independent antitumor effects that involve macrophages
    Hillary D Lum
    Department of Human Oncology, University of Wisconsin, Madison, WI 53792, USA
    J Leukoc Biol 79:1181-92. 2006
  6. pmc Macrophages are essential for antitumour effects against weakly immunogenic murine tumours induced by class B CpG-oligodeoxynucleotides
    Ilia N Buhtoiarov
    Department of Human Oncology, The University of Wisconsin School of Medicine and Public Health, Madison, WI 53792 4672, USA
    Immunology 120:412-23. 2007
  7. pmc Tumoristatic effects of anti-CD40 mAb-activated macrophages involve nitric oxide and tumour necrosis factor-alpha
    Hillary D Lum
    Department of Human Oncology, University of Wisconsin, Madison, WI 53792, USA
    Immunology 118:261-70. 2006
  8. pmc Intratumoral hu14.18-IL-2 (IC) induces local and systemic antitumor effects that involve both activated T and NK cells as well as enhanced IC retention
    Richard K Yang
    Department of Human Oncology, University of Wisconsin, Madison, WI 53705, USA
    J Immunol 189:2656-64. 2012
  9. pmc Anti-tumor and immunomodulatory activity of resveratrol in vitro and its potential for combining with cancer immunotherapy
    Brenda L Soto
    Department of Human Oncology, University of Wisconsin, Madison, WI 53706, USA
    Int Immunopharmacol 11:1877-86. 2011
  10. ncbi request reprint Enhanced activity of hu14.18-IL2 immunocytokine against murine NXS2 neuroblastoma when combined with interleukin 2 therapy
    Zane C Neal
    Department of Human Oncology, University of Wisconsin Madison, Madison, Wisconsin 53792, USA
    Clin Cancer Res 10:4839-47. 2004

Collaborators

Detail Information

Publications26

  1. ncbi request reprint Treatment of experimental breast cancer using interleukin-12 gene therapy combined with anti-vascular endothelial growth factor receptor-2 antibody
    Alexander L Rakhmilevich
    K4 413 Clinical Science Center, Department of Human Oncology, University of Wisconsin Madison, 600 Highland Avenue, Madison, WI 53792, USA
    Mol Cancer Ther 3:969-76. 2004
    ..Our results show a therapeutic benefit of combining IL-12 gene therapy and anti-VEGFR-2 mAb for cancer treatment...
  2. pmc Tumor-associated myeloid cells can be activated in vitro and in vivo to mediate antitumor effects
    Alexander L Rakhmilevich
    Department of Human Oncology, University of Wisconsin, 4136 WIMR, 1111 Highland Avenue, Madison, WI 53705, USA
    Cancer Immunol Immunother 61:1683-97. 2012
    ..Inhibition of NO did not abrogate the antitumor effects of stimulated myeloid cells. Taken together, the results indicate that in tumor-bearing hosts, tumor-associated myeloid cells can be activated to mediate antitumor effects...
  3. pmc T-cell-independent antitumor effects of CD40 ligation
    Alexander L Rakhmilevich
    Department of Human Oncology and Paul P Carbone Comprehensive Cancer Center, University of Wisconsin Madison, Madison, WI 53705, USA
    Int Rev Immunol 31:267-78. 2012
    ..In this review the authors focus on the ability of CD40 ligation to activate antitumor effector mechanisms of the cells of innate immunity such as macrophages and NK cells...
  4. doi request reprint CD40 ligation in vivo can induce T cell independent antitumor effects even against immunogenic tumors
    Alexander L Rakhmilevich
    The Department of Human Oncology, University of Wisconsin, K4 413 Clinical Science Center, 600 Highland Avenue, Madison, WI 53792, USA
    Cancer Immunol Immunother 57:1151-60. 2008
    ..Taken together, our results indicate that antitumor effects induced by alphaCD40, even against immunogenic tumors, can be observed in the absence of T cells and may involve macrophages...
  5. ncbi request reprint In vivo CD40 ligation can induce T-cell-independent antitumor effects that involve macrophages
    Hillary D Lum
    Department of Human Oncology, University of Wisconsin, Madison, WI 53792, USA
    J Leukoc Biol 79:1181-92. 2006
    ..Taken together, our results show that anti-CD40 mAb immunotherapy of mice can inhibit tumor growth in the absence of T cells, NK cells, and PMN through the involvement of activated Mphi...
  6. pmc Macrophages are essential for antitumour effects against weakly immunogenic murine tumours induced by class B CpG-oligodeoxynucleotides
    Ilia N Buhtoiarov
    Department of Human Oncology, The University of Wisconsin School of Medicine and Public Health, Madison, WI 53792 4672, USA
    Immunology 120:412-23. 2007
    ..Taken together, our results indicate that the growth control of weakly immunogenic tumours during CpG-immunotherapy is mediated predominantly by Mphi, rather than T cells or NK cells...
  7. pmc Tumoristatic effects of anti-CD40 mAb-activated macrophages involve nitric oxide and tumour necrosis factor-alpha
    Hillary D Lum
    Department of Human Oncology, University of Wisconsin, Madison, WI 53792, USA
    Immunology 118:261-70. 2006
    ..Taken together, these results suggest that NO and TNF-alpha are involved in, but not solely responsible for, the antitumour effects of macrophages after activation by CD40 ligation...
  8. pmc Intratumoral hu14.18-IL-2 (IC) induces local and systemic antitumor effects that involve both activated T and NK cells as well as enhanced IC retention
    Richard K Yang
    Department of Human Oncology, University of Wisconsin, Madison, WI 53705, USA
    J Immunol 189:2656-64. 2012
    ..v.-IC. Overall, IT-IC resulted in increased numbers of activated T and NK cells within tumors, better IC retention in the tumor, enhanced inhibition of tumor growth, and improved survival compared with i.v.-IC...
  9. pmc Anti-tumor and immunomodulatory activity of resveratrol in vitro and its potential for combining with cancer immunotherapy
    Brenda L Soto
    Department of Human Oncology, University of Wisconsin, Madison, WI 53706, USA
    Int Immunopharmacol 11:1877-86. 2011
    ..Results showed that this RV regimen inhibited the growth of NXS2 tumors in vivo but did not appear to interfere with blood cell count, splenocyte or macrophage function. Thus, RV may be a candidate for combining with immunotherapy...
  10. ncbi request reprint Enhanced activity of hu14.18-IL2 immunocytokine against murine NXS2 neuroblastoma when combined with interleukin 2 therapy
    Zane C Neal
    Department of Human Oncology, University of Wisconsin Madison, Madison, Wisconsin 53792, USA
    Clin Cancer Res 10:4839-47. 2004
    ..Animals showing prolonged tumor eradication of established tumors after the combined hu14.18-IL2 plus IL-2 regimen exhibited a protective T-cell-dependent antitumor memory response against NXS2 rechallenge...
  11. ncbi request reprint CD40 ligation activates murine macrophages via an IFN-gamma-dependent mechanism resulting in tumor cell destruction in vitro
    Ilia N Buhtoiarov
    Department of Human Oncology and UW Comprehensive Cancer Center, University of Wisconsin, Madison, WI 53792, USA
    J Immunol 174:6013-22. 2005
    ..IFN-gamma production by macrophages was dependent on IL-12. Taken together, the results show that murine macrophages are activated directly by anti-CD40 mAb to secrete IFN-gamma and mediate tumor cell destruction...
  12. pmc Intratumoral immunocytokine treatment results in enhanced antitumor effects
    Erik E Johnson
    Department of Surgery, University of Wisconsin, Madison, WI, 53792, USA
    Cancer Immunol Immunother 57:1891-902. 2008
    ..Further testing is required to determine how to potentially incorporate IT administration of IC into an antitumor regimen that optimizes local and systemic anticancer therapy...
  13. ncbi request reprint Synergistic activation of macrophages via CD40 and TLR9 results in T cell independent antitumor effects
    Ilia N Buhtoiarov
    Department of Human Oncology and Comprehensive Cancer Center, University of Wisconsin, Madison, WI 53792, USA
    J Immunol 176:309-18. 2006
    ....
  14. pmc Enhanced T-cell-independent antitumor effect of cyclophosphamide combined with anti-CD40 mAb and CpG in mice
    Erik E Johnson
    Paul P Carbone Comprehensive Cancer Center, Department of Surgery, University of Wisconsin, Madison, WI, USA
    J Immunother 34:76-84. 2011
    ..These results suggest that therapeutic strategies to activate macrophages may have potential for clinical application in cancer patients receiving chemotherapy...
  15. pmc Tumoricidal effects of activated macrophages in a mouse model of chronic lymphocytic leukemia
    Qing Li Wu
    Department of Pathology and Laboratory Medicine, Pediatrics University of Wisconsin School of Medicine and Public Health, Madison, WI 53792, USA
    J Immunol 182:6771-8. 2009
    ..Our results suggest a novel immunotherapeutic strategy for CLL that may be effective even in the face of tumor or chemotherapy-induced T cell immunodeficiency...
  16. pmc Naive mouse macrophages become activated following recognition of L5178Y lymphoma cells via concurrent ligation of CD40, NKG2D, and CD18 molecules
    Ilia N Buhtoiarov
    Department of Human Oncology, University of Wisconsin, Madison, WI 53792, USA
    J Immunol 182:1940-53. 2009
    ..These results suggest that contact with certain tumor cells via CD40, NKG2D, and CD18 molecules on the Mphi may facilitate Mphi-mediated antitumor immune surveillance...
  17. pmc Intratumoral treatment of smaller mouse neuroblastoma tumors with a recombinant protein consisting of IL-2 linked to the hu14.18 antibody increases intratumoral CD8+ T and NK cells and improves survival
    Richard K Yang
    The Department of Human Oncology, University of Wisconsin, Madison, WI 53705, USA
    Cancer Immunol Immunother 62:1303-13. 2013
    ..18-IL2 treatment is necessary for long-term survival. This model demonstrates that tumor size is a strong predictor of hu14.18-IL2-induced lymphocyte infiltration and treatment outcome...
  18. pmc The anti-tumor effect of resveratrol alone or in combination with immunotherapy in a neuroblastoma model
    Brenda L Soto
    Department of Human Oncology, University of Wisconsin, Madison, WI 53792, USA
    Cancer Immunol Immunother 60:731-8. 2011
    ..These results demonstrate that the combination therapy of peritumoral resveratrol plus intravenous immunocytokine provides better anti-tumor effects in this model than either therapy alone...
  19. pmc Anti-tumour synergy of cytotoxic chemotherapy and anti-CD40 plus CpG-ODN immunotherapy through repolarization of tumour-associated macrophages
    Ilia N Buhtoiarov
    Department of Human Oncology, University of Wisconsin, Madison, WI 53705 2275, USA
    Immunology 132:226-39. 2011
    ..Together, the results show that CT and anti-CD40 + CpG-ODN IT synergize in the induction of anti-tumour effects which are associated with the phenotypic repolarization of tumour-associated Mφ...
  20. pmc Radiofrequency ablation combined with KS-IL2 immunocytokine (EMD 273066) results in an enhanced antitumor effect against murine colon adenocarcinoma
    Erik E Johnson
    Departments of Surgery, Human Oncology, and Pediatrics and Paul P Carbone Comprehensive Cancer Center, University of Wisconsin, Madison, WI 53792, USA
    Clin Cancer Res 15:4875-84. 2009
    ..Radiofrequency ablation (RFA) is a common treatment modality for surgically unresectable tumors. However, there is a high rate of both local and systemic recurrence...
  21. pmc Intratumoral delivery of low doses of anti-CD40 mAb combined with monophosphoryl lipid a induces local and systemic antitumor effects in immunocompetent and T cell-deficient mice
    Tyler J Van De Voort
    Department of Human Oncology, University of Wisconsin Madison, Madison 53705 WI, USA
    J Immunother 36:29-40. 2013
    ..These preclinical data suggest that an anti-CD40+MPL combined regimen is appropriate for clinical testing in human patients, including cancer patients who may be immunosuppressed from prior chemotherapy...
  22. pmc Enhancement of the anti-melanoma response of Hu14.18K322A by αCD40 + CpG
    Kory L Alderson
    Department of Human Oncology, University of Wisconsin Madison, Madison, WI, USA
    Cancer Immunol Immunother 62:665-75. 2013
    ....
  23. pmc Differential internalization of hu14.18-IL2 immunocytokine by NK and tumor cell: impact on conjugation, cytotoxicity, and targeting
    Ilia N Buhtoiarov
    UW Carbone Cancer Center, 4159 MACC Fund UW Childhood Cancer Research Wing, WIMR, 1111 Highland Ave, Madison, WI 53705 2275, USA
    J Leukoc Biol 89:625-38. 2011
    ..These studies demonstrate that once IC binds to the tumor, it is present on the tumor surface for a prolonged time, inducing the recruitment of NK cells to the tumor site, followed by tumor cell killing...
  24. ncbi request reprint NXS2 murine neuroblastomas express increased levels of MHC class I antigens upon recurrence following NK-dependent immunotherapy
    Zane C Neal
    University of Wisconsin Comprehensive Cancer Center, University of Wisconsin Madison, 600 Highland Ave, K4 448, Madison, WI 53792, USA
    Cancer Immunol Immunother 53:41-52. 2004
    ....
  25. ncbi request reprint Intratumoral injection of interleukin-12 plasmid DNA, either naked or in complex with cationic lipid, results in similar tumor regression in a murine model
    Fushun Shi
    Department of Surgery, University of Wisconsin Madison, Madison, Wisconsin 53792, USA
    Mol Cancer Ther 1:949-57. 2002
    ..These results indicate that direct intratumoral gene transfer with naked nonviral IL-12 DNA provides an effective and simple method for the treatment of murine tumors, suggesting an approach for clinical application...
  26. doi request reprint Melanoma-induced suppression of tumor antigen-specific T cell expansion is comparable to suppression of global T cell expansion
    Andrew J Russ
    Section of Surgical Oncology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53792 7375, USA
    Cell Immunol 271:104-9. 2011
    ....