Malini Rajagopalan

Summary

Affiliation: University of Texas Health Center
Country: USA

Publications

  1. pmc Mycobacterium tuberculosis ClpX interacts with FtsZ and interferes with FtsZ assembly
    Renata Dziedzic
    The University of Texas Health Science Center, Tyler Biomedical Research, Tyler, Texas, United States of America
    PLoS ONE 5:e11058. 2010
  2. ncbi request reprint Mutations in the GTP-binding and synergy loop domains of Mycobacterium tuberculosis ftsZ compromise its function in vitro and in vivo
    Malini Rajagopalan
    Biomedical Research, The University of Texas Health Center at Tyler, 11937 U S Hwy 271, Tyler, TX 75708 3154, USA
    Biochem Biophys Res Commun 331:1171-7. 2005
  3. ncbi request reprint Genetic evidence that mycobacterial FtsZ and FtsW proteins interact, and colocalize to the division site in Mycobacterium smegmatis
    Malini Rajagopalan
    The University of Texas Health Center at Tyler, Biomedical Research, 11937 US Hwy 271, Tyler, TX 75708, United States
    FEMS Microbiol Lett 250:9-17. 2005
  4. pmc Septal localization of the Mycobacterium tuberculosis MtrB sensor kinase promotes MtrA regulon expression
    Renata Plocinska
    Biomedical Research, The University of Texas Health Science Center, Tyler, Texas 75708 3154, USA
    J Biol Chem 287:23887-99. 2012
  5. ncbi request reprint Interference of Mycobacterium tuberculosis cell division by Rv2719c, a cell wall hydrolase
    Ashwini Chauhan
    Biomedical Research, The University of Texas Health Center at Tyler, 11937 US Hwy 271, Tyler, TX 75708 3154, USA
    Mol Microbiol 62:132-47. 2006
  6. pmc Modulation of Mycobacterium tuberculosis DnaA protein-adenine-nucleotide interactions by acidic phospholipids
    Kohji Yamamoto
    Biomedical Research, The University of Texas Health Center at Tyler, 11937 U S Hwy 271, Tyler, TX 75708 3154, U S A
    Biochem J 363:305-11. 2002
  7. ncbi request reprint Conditional expression of Mycobacterium smegmatis ftsZ, an essential cell division gene
    Jaroslaw Dziadek
    Biomedical Research, The University of Texas Health Center at Tyler, 11937 US Hwy 271, Tyler, TX 75708, USA
    Microbiology 149:1593-603. 2003
  8. pmc Mycobacterium tuberculosis ftsZ expression and minimal promoter activity
    Manjot Kiran
    Biochemistry Department, The University of Texas Health Science Center Tyler, Tyler, TX 75708, USA
    Tuberculosis (Edinb) 89:S60-4. 2009
  9. pmc Synchronous replication initiation in novel Mycobacterium tuberculosis dnaA cold-sensitive mutants
    Naveen Nair
    Biomedical Research, Department of Biochemistry, The University of Texas Health Science Center at Tyler, Tyler, TX 75708 3154, USA
    Mol Microbiol 71:291-304. 2009
  10. pmc ChiZ levels modulate cell division process in mycobacteria
    Indumathi S Vadrevu
    Biomedical Research, The University of Texas Health Science Center, Tyler, TX, USA
    Tuberculosis (Edinb) 91:S128-35. 2011

Research Grants

Collaborators

Detail Information

Publications28

  1. pmc Mycobacterium tuberculosis ClpX interacts with FtsZ and interferes with FtsZ assembly
    Renata Dziedzic
    The University of Texas Health Science Center, Tyler Biomedical Research, Tyler, Texas, United States of America
    PLoS ONE 5:e11058. 2010
    ..Taken together, our results suggest that M. tuberculosis ClpX interacts stoichiometrically with FtsZ protomers, independent of its nucleotide-bound state and negatively regulates FtsZ activities, hence cell division...
  2. ncbi request reprint Mutations in the GTP-binding and synergy loop domains of Mycobacterium tuberculosis ftsZ compromise its function in vitro and in vivo
    Malini Rajagopalan
    Biomedical Research, The University of Texas Health Center at Tyler, 11937 U S Hwy 271, Tyler, TX 75708 3154, USA
    Biochem Biophys Res Commun 331:1171-7. 2005
    ..Together, our results indicate that optimal GTPase and polymerization activities of FtsZ are required to sustain cell division in mycobacteria and that the same conserved mutations in different bacterial species have distinct phenotypes...
  3. ncbi request reprint Genetic evidence that mycobacterial FtsZ and FtsW proteins interact, and colocalize to the division site in Mycobacterium smegmatis
    Malini Rajagopalan
    The University of Texas Health Center at Tyler, Biomedical Research, 11937 US Hwy 271, Tyler, TX 75708, United States
    FEMS Microbiol Lett 250:9-17. 2005
    ..Our results suggest that mycobacterial FtsZ can localize to the septum independent of FtsW, and that interactions of FtsW with FtsZ are critical for the formation of productive FtsZ-rings and the cell division process in mycobacteria...
  4. pmc Septal localization of the Mycobacterium tuberculosis MtrB sensor kinase promotes MtrA regulon expression
    Renata Plocinska
    Biomedical Research, The University of Texas Health Science Center, Tyler, Texas 75708 3154, USA
    J Biol Chem 287:23887-99. 2012
    ..We propose that MtrB septal association is a necessary feature of MtrB activation that promotes MtrA phosphorylation and MtrA regulon expression...
  5. ncbi request reprint Interference of Mycobacterium tuberculosis cell division by Rv2719c, a cell wall hydrolase
    Ashwini Chauhan
    Biomedical Research, The University of Texas Health Center at Tyler, 11937 US Hwy 271, Tyler, TX 75708 3154, USA
    Mol Microbiol 62:132-47. 2006
    ....
  6. pmc Modulation of Mycobacterium tuberculosis DnaA protein-adenine-nucleotide interactions by acidic phospholipids
    Kohji Yamamoto
    Biomedical Research, The University of Texas Health Center at Tyler, 11937 U S Hwy 271, Tyler, TX 75708 3154, U S A
    Biochem J 363:305-11. 2002
    ..tuberculosis oriC involves intimate interactions between DnaA, adenine nucleotides and membrane phospholipids, and the latter helps to ensure that only the ATP form of the DnaA protein interacts continuously with oriC...
  7. ncbi request reprint Conditional expression of Mycobacterium smegmatis ftsZ, an essential cell division gene
    Jaroslaw Dziadek
    Biomedical Research, The University of Texas Health Center at Tyler, 11937 US Hwy 271, Tyler, TX 75708, USA
    Microbiology 149:1593-603. 2003
    ..It is concluded that optimal levels of M. smegmatis FtsZ are required to sustain cell division and that the cell division initiation mechanisms are similar in mycobacteria...
  8. pmc Mycobacterium tuberculosis ftsZ expression and minimal promoter activity
    Manjot Kiran
    Biochemistry Department, The University of Texas Health Science Center Tyler, Tyler, TX 75708, USA
    Tuberculosis (Edinb) 89:S60-4. 2009
    ..We propose that ftsZ expression from all promoters is required for optimal intracellular FtsZ levels and that the activities of P4 and possibly other promoters are down-regulated during growth-arrest conditions...
  9. pmc Synchronous replication initiation in novel Mycobacterium tuberculosis dnaA cold-sensitive mutants
    Naveen Nair
    Biomedical Research, Department of Biochemistry, The University of Texas Health Science Center at Tyler, Tyler, TX 75708 3154, USA
    Mol Microbiol 71:291-304. 2009
    ..tuberculosis requires that the dnaA promoter remains active during the replication period and that the DnaA protein is able to interact with ATP...
  10. pmc ChiZ levels modulate cell division process in mycobacteria
    Indumathi S Vadrevu
    Biomedical Research, The University of Texas Health Science Center, Tyler, TX, USA
    Tuberculosis (Edinb) 91:S128-35. 2011
    ..Our data suggest that optimal levels and activity of the cell wall hydrolase ChiZ are required for regulated cell division in mycobacteria...
  11. pmc Mycobacterium tuberculosis origin of replication and the promoter for immunodominant secreted antigen 85B are the targets of MtrA, the essential response regulator
    Malini Rajagopalan
    Biomedical Research, University of Texas Health Science Center, Tyler, Texas 75708 3154, USA
    J Biol Chem 285:15816-27. 2010
    ..e. oriC and fbpB, reflects its main role as a coordinator between the proliferative and pathogenic functions of Mtb...
  12. ncbi request reprint The intrinsic ATPase activity of Mycobacterium tuberculosis DnaA promotes rapid oligomerization of DnaA on oriC
    Murty V V S Madiraju
    Biomedical Research, The University of Texas Health Center at Tyler, 75708 3154, USA
    Mol Microbiol 59:1876-90. 2006
    ..We propose that ATPase activity enables the DnaA protomers on oriC to rapidly form oligomeric complexes competent for replication initiation...
  13. pmc Replacement of Mycobacterium smegmatis dnaA gene by Mycobacterium tuberculosis homolog results in temperature sensitivity
    Murty Madiraju
    The University of Texas Health Science Center, 11937 US Hwy 271, Tyler, TX 75703, United States
    Tuberculosis (Edinb) 91:S136-41. 2011
    ..Our results suggest that Mtb DnaA functions as a partially active protein in M. smegmatis, hence is not as proficient as M. smegmatis counterpart in optimally driving the M. smegmatis oriC replication machinery...
  14. ncbi request reprint Physiological consequences associated with overproduction of Mycobacterium tuberculosis FtsZ in mycobacterial hosts
    Jaroslaw Dziadek
    Biomedical Research, The University of Texas Health Center at Tyler, 11937 US Hwy 271, Tyler, TX 75708 3154, USA
    Microbiology 148:961-71. 2002
    ..Together these results suggest that the intracellular concentration of FtsZ protein is critical for productive septum formation in mycobacteria...
  15. pmc The two-domain LysX protein of Mycobacterium tuberculosis is required for production of lysinylated phosphatidylglycerol and resistance to cationic antimicrobial peptides
    Erin Maloney
    Department of Biochemistry, The University of Texas Health Center at Tyler, Tyler, TX, USA
    PLoS Pathog 5:e1000534. 2009
    ..Together, our results suggest that LysX-mediated production of L-PG is necessary for the maintenance of optimal membrane integrity and for survival of the pathogen upon infection...
  16. ncbi request reprint Modulation of Mycobacterium tuberculosis proliferation by MtrA, an essential two-component response regulator
    Marek Fol
    Biomedical Research, The University of Texas Health Center at Tyler, 11937 U S Hwy 271, Tyler, TX 75708 3154, USA
    Mol Microbiol 60:643-57. 2006
    ..We propose that proliferation of M. tuberculosis in vivo depends, in part, on the optimal ratio of phosphorylated to non-phosphorylated MtrA response regulator...
  17. pmc Mycobacterium tuberculosis ftsH expression in response to stress and viability
    Manjot Kiran
    Biochemistry Department, The University of Texas Health Science Center at Tyler, 11937 US Hwy 271, Tyler, TX 75708, USA
    Tuberculosis (Edinb) 89:S70-3. 2009
    ..Together, our results suggest that Mtb FtsH is a stress-response protein that promotes the pathogen's ability to deal with ROI stress and is possibly involved in the regulation of FtsZ levels...
  18. ncbi request reprint Conditional expression of Mycobacterium smegmatis dnaA, an essential DNA replication gene
    Rebecca Greendyke
    Biomedical Research, 11937 US The University of Texas Health Center at Tyler, Tyler, TX 75708 3154, USA
    Microbiology 148:3887-900. 2002
    ..It is concluded that DNA replication and cell-division processes in M. smegmatis are linked, and it is proposed that DnaA has a role in both of these processes...
  19. pmc Overproduction and localization of Mycobacterium tuberculosis ParA and ParB proteins
    Erin Maloney
    Biochemistry Department, The University of Texas Health Science Center at Tyler, Tyler, TX 75708, USA
    Tuberculosis (Edinb) 89:S65-9. 2009
    ..Collectively our results suggest that the M. tuberculosis Par proteins play important roles in cell-cycle progression...
  20. pmc Mycobacterium tuberculosis cells growing in macrophages are filamentous and deficient in FtsZ rings
    Ashwini Chauhan
    Biomedical Research, The University of Texas Health Center at Tyler, Tyler, TX 75708 3154, USA
    J Bacteriol 188:1856-65. 2006
    ..Our results suggest that the intraphagosomal milieu alters the expression of M. tuberculosis genes affecting Z-ring formation and thereby cell division...
  21. pmc Localization of acidic phospholipid cardiolipin and DnaA in mycobacteria
    Erin Maloney
    Biomedical Research, The University of Texas Health Science Center, 11937 US Hwy 271, Tyler, TX 75708 3154, United States
    Tuberculosis (Edinb) 91:S150-5. 2011
    ..Finally, we show that the localization pattern of the DnaA-green fluorescent fusion protein is similar to CL. Our results suggest that DnaA colocalizes with CL during cell cycle progression...
  22. pmc Mycobacterium tuberculosis mtrA merodiploid strains with point mutations in the signal-receiving domain of MtrA exhibit growth defects in nutrient broth
    Maha Al Zayer
    Biomedical Research, The University of Texas Health Science Center at Tyler, Tyler, TX 75708, USA
    Plasmid 65:210-8. 2011
    ..Our results suggest that MtrA(D13A) behaves like a constitutively active response regulator and that further characterization of mtrA merodiploid strains will provide valuable clues to the MtrAB system...
  23. pmc Mutations in the CCGTTCACA DnaA box of Mycobacterium tuberculosis oriC that abolish replication of oriC plasmids are tolerated on the chromosome
    Jaroslaw Dziadek
    Department of Biochemistry, The University of Texas Health Center at Tyler, 75708 3154, USA
    J Bacteriol 184:3848-55. 2002
    ..tuberculosis strains have evolved mechanisms to tolerate mutations in the oriC region and that functional requirements for M. tuberculosis oriC replication are different for chromosomes and plasmids...
  24. ncbi request reprint Phospholipids promote dissociation of ADP from the Mycobacterium avium DnaA protein
    Kohji Yamamoto
    Department of Biochemistry, The University of Texas Health Center at Tyler, Tyler, TX 75703 3154, USA
    J Biochem 131:219-24. 2002
    ..avium origin of replication. We suggest that the initiation of DNA replication in M. avium involves an interplay among DnaA, adenine nucleotides and phospholipids...
  25. doi request reprint Mycobacterium tuberculosis MtrAY102C is a gain-of-function mutant that potentially acts as a constitutively active protein
    Akash T Satsangi
    Biomedical Research, The University of Texas Health Science Center Tyler, 11937 U S Hwy 271, Tyler, TX 75708 3154, USA
    Tuberculosis (Edinb) 93:S28-32. 2013
    ..Together, these results support a hypothesis that the gain-of-function phenotype of MtrAY102C is in part due to its ability to function as a constitutively active protein in the absence of phosphorylation. ..
  26. pmc Characterization of CrgA, a new partner of the Mycobacterium tuberculosis peptidoglycan polymerization complexes
    P Plocinski
    The University of Texas Health Science Center, Tyler, TX 75708, USA
    J Bacteriol 193:3246-56. 2011
    ..Overall, these data indicate that CrgA is a novel member of the cell division complex in mycobacteria and possibly facilitates septum formation...
  27. pmc Facilitation of dissociation reaction of nucleotides bound to Mycobacterium tuberculosis DnaA
    Kohji Yamamoto
    Graduate School of Bioresource and Bioenvironmental Science, Kyushu University, Fukuoka 812 8581, Japan
    J Biochem 143:759-64. 2008
    ..We suggest that the outcome of intra-cellular DnaA(TB)-nucleotide interactions, hence DnaA(TB) activity, is influenced by phospholipids...
  28. pmc Assembly dynamics of Mycobacterium tuberculosis FtsZ
    Yaodong Chen
    Department of Cell Biology, Duke University, Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 282:27736-43. 2007
    ..7 was 42 s for MtbFtsZ compared with 5.5 s for EcFtsZ. Photobleaching studies in vivo showed a range of turnover half-times with an average of 25 s for MtbFtsZ as compared with 9 s for EcFtsZ...

Research Grants10

  1. MtrA and Mycobacterium tuberculosis proliferation
    Malini Rajagopalan; Fiscal Year: 2007
    ..These experiments will establish whether oriC replication is a target of MtrA system and will improve our understanding of regulation of Mtb multiplication in vivo. ..
  2. Mycobacterium tuberculosis cell division and proliferation
    Malini Rajagopalan; Fiscal Year: 2010
    ..It is hoped that these experiments will define how the cell division process in Mtb is regulated and will advance our understanding of proliferation of Mtb in vivo. ..
  3. Mycobacterium tuberculosis cell division and proliferation
    Malini Rajagopalan; Fiscal Year: 2009
    ..It is hoped that these experiments will define how the cell division process in Mtb is regulated and will advance our understanding of proliferation of Mtb in vivo. ..
  4. Mycobacterium tuberculosis cell division and proliferation
    Malini Rajagopalan; Fiscal Year: 2007
    ..It is hoped that these experiments will define how the cell division process in Mtb is regulated and will advance our understanding of proliferation of Mtb in vivo. ..