Research Topics
| Malini RajagopalanSummaryAffiliation: University of Texas Health Center Country: USA Publications
Research Grants
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Detail Information
Publications
Genetic evidence that mycobacterial FtsZ and FtsW proteins interact, and colocalize to the division site in Mycobacterium smegmatisMalini Rajagopalan
The University of Texas Health Center at Tyler, Biomedical Research, 11937 US Hwy 271, Tyler, TX 75708, United States
FEMS Microbiol Lett 250:9-17. 2005..Our results suggest that mycobacterial FtsZ can localize to the septum independent of FtsW, and that interactions of FtsW with FtsZ are critical for the formation of productive FtsZ-rings and the cell division process in mycobacteria...- Mutations in the GTP-binding and synergy loop domains of Mycobacterium tuberculosis ftsZ compromise its function in vitro and in vivoMalini Rajagopalan
Biomedical Research, The University of Texas Health Center at Tyler, 11937 U S Hwy 271, Tyler, TX 75708 3154, USA
Biochem Biophys Res Commun 331:1171-7. 2005..Together, our results indicate that optimal GTPase and polymerization activities of FtsZ are required to sustain cell division in mycobacteria and that the same conserved mutations in different bacterial species have distinct phenotypes... - Modulation of Mycobacterium tuberculosis DnaA protein-adenine-nucleotide interactions by acidic phospholipidsKohji Yamamoto
Biomedical Research, The University of Texas Health Center at Tyler, 11937 U.S. Hwy @ 271, Tyler, TX 75708-3154, U.S.A
Biochem J 363:305-11. 2002..tuberculosis oriC involves intimate interactions between DnaA, adenine nucleotides and membrane phospholipids, and the latter helps to ensure that only the ATP form of the DnaA protein interacts continuously with oriC... - Septal localization of the Mycobacterium tuberculosis MtrB sensor kinase promotes MtrA regulon expressionRenata Plocinska
Biomedical Research, The University of Texas Health Science Center, Tyler, Texas 75708 3154, USA
J Biol Chem 287:23887-99. 2012..We propose that MtrB septal association is a necessary feature of MtrB activation that promotes MtrA phosphorylation and MtrA regulon expression... - Interference of Mycobacterium tuberculosis cell division by Rv2719c, a cell wall hydrolaseAshwini Chauhan
Biomedical Research, The University of Texas Health Center at Tyler, 11937 US Hwy 271, Tyler, TX 75708-3154, USA
Mol Microbiol 62:132-47. 2006.... - Conditional expression of Mycobacterium smegmatis ftsZ, an essential cell division geneJaroslaw Dziadek
Biomedical Research, The University of Texas Health Center at Tyler, 11937 US Hwy 271, Tyler, TX 75708, USA
Microbiology 149:1593-603. 2003..It is concluded that optimal levels of M. smegmatis FtsZ are required to sustain cell division and that the cell division initiation mechanisms are similar in mycobacteria... - ChiZ levels modulate cell division process in mycobacteriaIndumathi S Vadrevu
Biomedical Research, The University of Texas Health Science Center, Tyler, TX, USA
Tuberculosis (Edinb) 91:S128-35. 2011..Our data suggest that optimal levels and activity of the cell wall hydrolase ChiZ are required for regulated cell division in mycobacteria... - Mycobacterium tuberculosis origin of replication and the promoter for immunodominant secreted antigen 85B are the targets of MtrA, the essential response regulatorMalini Rajagopalan
Biomedical Research, University of Texas Health Science Center, Tyler, Texas 75708 3154, USA
J Biol Chem 285:15816-27. 2010..e. oriC and fbpB, reflects its main role as a coordinator between the proliferative and pathogenic functions of Mtb... - Mycobacterium tuberculosis ftsZ expression and minimal promoter activityManjot Kiran
Biochemistry Department, The University of Texas Health Science Center Tyler, Tyler, TX 75708, USA
Tuberculosis (Edinb) 89:S60-4. 2009..We propose that ftsZ expression from all promoters is required for optimal intracellular FtsZ levels and that the activities of P4 and possibly other promoters are down-regulated during growth-arrest conditions... - Replacement of Mycobacterium smegmatis dnaA gene by Mycobacterium tuberculosis homolog results in temperature sensitivityMurty Madiraju
The University of Texas Health Science Center, 11937 US Hwy 271, Tyler, TX 75703, United States
Tuberculosis (Edinb) 91:S136-41. 2011..Our results suggest that Mtb DnaA functions as a partially active protein in M. smegmatis, hence is not as proficient as M. smegmatis counterpart in optimally driving the M. smegmatis oriC replication machinery... - Mycobacterium tuberculosis ftsH expression in response to stress and viabilityManjot Kiran
Biochemistry Department, The University of Texas Health Science Center at Tyler, 11937 US Hwy 271, Tyler, TX 75708, USA
Tuberculosis (Edinb) 89:S70-3. 2009..Together, our results suggest that Mtb FtsH is a stress-response protein that promotes the pathogen's ability to deal with ROI stress and is possibly involved in the regulation of FtsZ levels... - The two-domain LysX protein of Mycobacterium tuberculosis is required for production of lysinylated phosphatidylglycerol and resistance to cationic antimicrobial peptidesErin Maloney
Department of Biochemistry, The University of Texas Health Center at Tyler, Tyler, TX, USA
PLoS Pathog 5:e1000534. 2009..Together, our results suggest that LysX-mediated production of L-PG is necessary for the maintenance of optimal membrane integrity and for survival of the pathogen upon infection... - Synchronous replication initiation in novel Mycobacterium tuberculosis dnaA cold-sensitive mutantsNaveen Nair
Biomedical Research, Department of Biochemistry, The University of Texas Health Science Center at Tyler, Tyler, TX 75708 3154, USA
Mol Microbiol 71:291-304. 2009..tuberculosis requires that the dnaA promoter remains active during the replication period and that the DnaA protein is able to interact with ATP... - Physiological consequences associated with overproduction of Mycobacterium tuberculosis FtsZ in mycobacterial hostsJaroslaw Dziadek
Biomedical Research, The University of Texas Health Center at Tyler, 11937 US Hwy @ 271, Tyler, TX 75708-3154, USA
Microbiology 148:961-71. 2002..Together these results suggest that the intracellular concentration of FtsZ protein is critical for productive septum formation in mycobacteria... - Modulation of Mycobacterium tuberculosis proliferation by MtrA, an essential two-component response regulatorMarek Fol
Biomedical Research, The University of Texas Health Center at Tyler, 11937 U S Hwy 271, Tyler, TX 75708 3154, USA
Mol Microbiol 60:643-57. 2006..We propose that proliferation of M. tuberculosis in vivo depends, in part, on the optimal ratio of phosphorylated to non-phosphorylated MtrA response regulator... - Conditional expression of Mycobacterium smegmatis dnaA, an essential DNA replication geneRebecca Greendyke
Biomedical Research, 11937 US The University of Texas Health Center at Tyler, Tyler, TX-75708-3154, USA
Microbiology 148:3887-900. 2002..It is concluded that DNA replication and cell-division processes in M. smegmatis are linked, and it is proposed that DnaA has a role in both of these processes... - Overproduction and localization of Mycobacterium tuberculosis ParA and ParB proteinsErin Maloney
Biochemistry Department, The University of Texas Health Science Center at Tyler, Tyler, TX 75708, USA
Tuberculosis (Edinb) 89:S65-9. 2009..Collectively our results suggest that the M. tuberculosis Par proteins play important roles in cell-cycle progression... - The intrinsic ATPase activity of Mycobacterium tuberculosis DnaA promotes rapid oligomerization of DnaA on oriCMurty V V S Madiraju
Biomedical Research, The University of Texas Health Center at Tyler, 75708 3154, USA
Mol Microbiol 59:1876-90. 2006..We propose that ATPase activity enables the DnaA protomers on oriC to rapidly form oligomeric complexes competent for replication initiation... - Mycobacterium tuberculosis cells growing in macrophages are filamentous and deficient in FtsZ ringsAshwini Chauhan
Biomedical Research, The University of Texas Health Center at Tyler, Tyler, TX 75708-3154, USA
J Bacteriol 188:1856-65. 2006..Our results suggest that the intraphagosomal milieu alters the expression of M. tuberculosis genes affecting Z-ring formation and thereby cell division... - Localization of acidic phospholipid cardiolipin and DnaA in mycobacteriaErin Maloney
Biomedical Research, The University of Texas Health Science Center, 11937 US Hwy 271, Tyler, TX 75708 3154, United States
Tuberculosis (Edinb) 91:S150-5. 2011..Finally, we show that the localization pattern of the DnaA-green fluorescent fusion protein is similar to CL. Our results suggest that DnaA colocalizes with CL during cell cycle progression... - Mycobacterium tuberculosis mtrA merodiploid strains with point mutations in the signal-receiving domain of MtrA exhibit growth defects in nutrient brothMaha Al Zayer
Biomedical Research, The University of Texas Health Science Center at Tyler, Tyler, TX 75708, USA
Plasmid 65:210-8. 2011..Our results suggest that MtrA(D13A) behaves like a constitutively active response regulator and that further characterization of mtrA merodiploid strains will provide valuable clues to the MtrAB system... - Mutations in the CCGTTCACA DnaA box of Mycobacterium tuberculosis oriC that abolish replication of oriC plasmids are tolerated on the chromosomeJaroslaw Dziadek
Department of Biochemistry, The University of Texas Health Center at Tyler, 75708-3154, USA
J Bacteriol 184:3848-55. 2002..tuberculosis strains have evolved mechanisms to tolerate mutations in the oriC region and that functional requirements for M. tuberculosis oriC replication are different for chromosomes and plasmids... - Phospholipids promote dissociation of ADP from the Mycobacterium avium DnaA proteinKohji Yamamoto
Department of Biochemistry, The University of Texas Health Center at Tyler, Tyler, TX 75703-3154, USA
J Biochem (Tokyo) 131:219-24. 2002..avium origin of replication. We suggest that the initiation of DNA replication in M. avium involves an interplay among DnaA, adenine nucleotides and phospholipids... - Characterization of CrgA, a new partner of the Mycobacterium tuberculosis peptidoglycan polymerization complexesP Plocinski
The University of Texas Health Science Center, Tyler, TX 75708, USA
J Bacteriol 193:3246-56. 2011..Overall, these data indicate that CrgA is a novel member of the cell division complex in mycobacteria and possibly facilitates septum formation... - Assembly dynamics of Mycobacterium tuberculosis FtsZYaodong Chen
Department of Cell Biology, Duke University, Medical Center, Durham, North Carolina 27710, USA
J Biol Chem 282:27736-43. 2007..7 was 42 s for MtbFtsZ compared with 5.5 s for EcFtsZ. Photobleaching studies in vivo showed a range of turnover half-times with an average of 25 s for MtbFtsZ as compared with 9 s for EcFtsZ... - Facilitation of dissociation reaction of nucleotides bound to Mycobacterium tuberculosis DnaAKohji Yamamoto
Graduate School of Bioresource and Bioenvironmental Science, Kyushu University, Fukuoka 812 8581, Japan
J Biochem 143:759-64. 2008..We suggest that the outcome of intra-cellular DnaA(TB)-nucleotide interactions, hence DnaA(TB) activity, is influenced by phospholipids...
Research Grants
- MtrA and Mycobacterium tuberculosis proliferationMalini Rajagopalan; Fiscal Year: 2007..These experiments will establish whether oriC replication is a target of MtrA system and will improve our understanding of regulation of Mtb multiplication in vivo. ..
- Mycobacterium tuberculosis cell division and proliferationMalini Rajagopalan; Fiscal Year: 2010..It is hoped that these experiments will define how the cell division process in Mtb is regulated and will advance our understanding of proliferation of Mtb in vivo. ..
- Mycobacterium tuberculosis cell division and proliferationMalini Rajagopalan; Fiscal Year: 2009..It is hoped that these experiments will define how the cell division process in Mtb is regulated and will advance our understanding of proliferation of Mtb in vivo. ..
- Mycobacterium tuberculosis cell division and proliferationMalini Rajagopalan; Fiscal Year: 2007..It is hoped that these experiments will define how the cell division process in Mtb is regulated and will advance our understanding of proliferation of Mtb in vivo. ..