J M Rae

Summary

Affiliation: University of Michigan
Country: USA

Publications

  1. ncbi request reprint Endoxifen, a secondary metabolite of tamoxifen, and 4-OH-tamoxifen induce similar changes in global gene expression patterns in MCF-7 breast cancer cells
    Young Chai Lim
    Division of Clinical Pharmacology, Indiana University School of Medicine, 1001 West 10th Street, WD Myers Bldg, W7123, Indianapolis, IN 46202, USA
    J Pharmacol Exp Ther 318:503-12. 2006
  2. pmc Concordance between CYP2D6 genotypes obtained from tumor-derived and germline DNA
    James M Rae
    Breast Oncology Program, University of Michigan Comprehensive Cancer Center, University of Michigan, Ann Arbor, MI 48109 5942, USA
    J Natl Cancer Inst 105:1332-4. 2013
  3. pmc CYP2D6 and UGT2B7 genotype and risk of recurrence in tamoxifen-treated breast cancer patients
    James M Rae
    Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI, USA
    J Natl Cancer Inst 104:452-60. 2012
  4. pmc When will tumor gene expression profiling be incorporated into clinical breast cancer decision making?
    Chad J Creighton
    Bioinformatics Program, University of Michigan Medical Center, Ann Arbor, Michigan, USA
    Breast Cancer Res 8:302. 2006
  5. pmc CYP2D6 genotype and tamoxifen response
    James M Rae
    Breast Cancer Res 7:E6. 2005
  6. pmc What does an orphan G-protein-coupled receptor have to do with estrogen?
    James M Rae
    Division of Hematology Oncology, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, Michigan, USA
    Breast Cancer Res 7:243-4. 2005
  7. pmc The role of single nucleotide polymorphisms in breast cancer metastasis
    James M Rae
    Division of Hematology Oncology, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109, USA
    Breast Cancer Res 10:301. 2008
  8. ncbi request reprint GREB1 is a novel androgen-regulated gene required for prostate cancer growth
    James M Rae
    Division of Hematology Oncology, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, Michigan 48109, USA
    Prostate 66:886-94. 2006
  9. pmc Cytochrome P450 2D6 activity predicts discontinuation of tamoxifen therapy in breast cancer patients
    J M Rae
    Breast Oncology Program, Department of Internal Medicine, University of Michigan Comprehensive Cancer Center, Ann Arbor, MI 48109 0612, USA
    Pharmacogenomics J 9:258-64. 2009
  10. ncbi request reprint EGFR and EGFRvIII expression in primary breast cancer and cell lines
    James M Rae
    Department of Internal Medicine, Division of Hematology Oncology, University of Michigan Medical Center, Ann Arbor, MI 48109 0612, USA
    Breast Cancer Res Treat 87:87-95. 2004

Detail Information

Publications45

  1. ncbi request reprint Endoxifen, a secondary metabolite of tamoxifen, and 4-OH-tamoxifen induce similar changes in global gene expression patterns in MCF-7 breast cancer cells
    Young Chai Lim
    Division of Clinical Pharmacology, Indiana University School of Medicine, 1001 West 10th Street, WD Myers Bldg, W7123, Indianapolis, IN 46202, USA
    J Pharmacol Exp Ther 318:503-12. 2006
    ..We conclude that endoxifen and 4-OH-Tam have similar effects on global gene expression patterns in MCF-7 cells and that the majority of the affected genes are estrogen-regulated genes...
  2. pmc Concordance between CYP2D6 genotypes obtained from tumor-derived and germline DNA
    James M Rae
    Breast Oncology Program, University of Michigan Comprehensive Cancer Center, University of Michigan, Ann Arbor, MI 48109 5942, USA
    J Natl Cancer Inst 105:1332-4. 2013
    ..We conclude that CYP2D6 genotypes obtained from FFPETs accurately represent the patient's CYP2D6 metabolic phenotype. ..
  3. pmc CYP2D6 and UGT2B7 genotype and risk of recurrence in tamoxifen-treated breast cancer patients
    James M Rae
    Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI, USA
    J Natl Cancer Inst 104:452-60. 2012
    ..Previous studies have suggested that metabolic conversion of tamoxifen to endoxifen by cytochrome P450 2D6 (CYP2D6) is required for patient benefit from tamoxifen therapy...
  4. pmc When will tumor gene expression profiling be incorporated into clinical breast cancer decision making?
    Chad J Creighton
    Bioinformatics Program, University of Michigan Medical Center, Ann Arbor, Michigan, USA
    Breast Cancer Res 8:302. 2006
    ..Recent gene expression profiling studies using cell line models to identify downstream transcriptional targets of oncogenic signaling pathways may help achieve this goal...
  5. pmc CYP2D6 genotype and tamoxifen response
    James M Rae
    Breast Cancer Res 7:E6. 2005
  6. pmc What does an orphan G-protein-coupled receptor have to do with estrogen?
    James M Rae
    Division of Hematology Oncology, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, Michigan, USA
    Breast Cancer Res 7:243-4. 2005
    ..It has long been postulated that the rapid effects of estrogen are due to a membrane-bound ER, and two recent reports suggest that it is in fact a G-protein-coupled receptor named 'GPR30'...
  7. pmc The role of single nucleotide polymorphisms in breast cancer metastasis
    James M Rae
    Division of Hematology Oncology, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109, USA
    Breast Cancer Res 10:301. 2008
    ..Although some provocative studies suggest potential candidates for metastasis regulating genes, the conclusive identification of a specific inherited genetic variant that alters metastatic potential awaits further studies...
  8. ncbi request reprint GREB1 is a novel androgen-regulated gene required for prostate cancer growth
    James M Rae
    Division of Hematology Oncology, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, Michigan 48109, USA
    Prostate 66:886-94. 2006
    ..GREB1 is expressed in the prostate and its putative promoter contains potential androgen receptor (AR) response elements...
  9. pmc Cytochrome P450 2D6 activity predicts discontinuation of tamoxifen therapy in breast cancer patients
    J M Rae
    Breast Oncology Program, Department of Internal Medicine, University of Michigan Comprehensive Cancer Center, Ann Arbor, MI 48109 0612, USA
    Pharmacogenomics J 9:258-64. 2009
    ..Therefore, patients who may be most likely to benefit from tamoxifen may paradoxically be most likely to discontinue treatment prematurely...
  10. ncbi request reprint EGFR and EGFRvIII expression in primary breast cancer and cell lines
    James M Rae
    Department of Internal Medicine, Division of Hematology Oncology, University of Michigan Medical Center, Ann Arbor, MI 48109 0612, USA
    Breast Cancer Res Treat 87:87-95. 2004
    ..In contrast, EGFRwt was expressed at varying levels in the majority of samples tested. We conclude that the expression of EGFRvIII is extremely rare in breast cancer and therefore it does not contribute to the malignant phenotype...
  11. ncbi request reprint GREB 1 is a critical regulator of hormone dependent breast cancer growth
    James M Rae
    Department of Internal Medicine, Division of Hematology and Oncology, University of Michigan Medical Center, 1150 W Medical Center Drive, Ann Arbor, MI 48109 0612, USA
    Breast Cancer Res Treat 92:141-9. 2005
    ....
  12. ncbi request reprint Evaluation of novel epidermal growth factor receptor tyrosine kinase inhibitors
    James M Rae
    Department of Oncology, Georgetown University Medical Center, Washington, DC, USA
    Breast Cancer Res Treat 83:99-107. 2004
    ..5 microM or less. However, higher doses (EC50's >or= 2 microM) were needed to block the growth of human tumor cell lines potentially implicating a second site of action for these compounds...
  13. ncbi request reprint MDA-MB-435 cells are derived from M14 melanoma cells--a loss for breast cancer, but a boon for melanoma research
    James M Rae
    Division of Hematology Oncology, Department of Internal Medicine, University of Michigan Medical Center, 1150 West Medical Center Drive, Med Sci I, Room 5323, Ann Arbor, MI 48109 0612, USA
    Breast Cancer Res Treat 104:13-9. 2007
    ..We could not determine, however, whether the melanoma-like properties of the MDA-MB-435 cell line are the result of misclassification or due to transdifferention to a melanoma-like phenotype...
  14. ncbi request reprint Genotyping for polymorphic drug metabolizing enzymes from paraffin-embedded and immunohistochemically stained tumor samples
    James M Rae
    Division of Hematology Oncology, Department of Internal Medicine, University of Michigan Medical Center, 5323 Med Sci 1, 1150 W Medical Center Drive, Ann Arbor, MI 48109, USA
    Pharmacogenetics 13:501-7. 2003
    ..We set out to establish genotyping methods for relevant genes from archival tumor samples and determine if fixation, processing or somatic changes in the tumor might affect our ability to identify germ-line polymorphisms...
  15. ncbi request reprint Triethylenethiophosphoramide is a specific inhibitor of cytochrome P450 2B6: implications for cyclophosphamide metabolism
    James M Rae
    Division of Clinical Pharmacology, Department of Medicine, Georgetown University Medical Center, Washington, DC, USA
    Drug Metab Dispos 30:525-30. 2002
    ..Furthermore, thioTEPA may prove to be a valuable new tool for the study of this important drug-metabolizing enzyme...
  16. doi request reprint Estrogen receptor genotypes, menopausal status, and the effects of tamoxifen on lipid levels: revised and updated results
    D F Hayes
    Department of Internal Medicine and Breast Oncology Program, Comprehensive Cancer Center, University of Michigan Health and Hospitals System, Ann Arbor, Michigan, USA
    Clin Pharmacol Ther 88:626-9. 2010
    ....
  17. pmc Lack of association between oestrogen receptor polymorphisms and change in bone mineral density with tamoxifen therapy
    N L Henry
    Breast Oncology Program, University of Michigan Comprehensive Cancer Center, Ann Arbor, MI 48109, USA
    Br J Cancer 102:294-300. 2010
    ..We hypothesised that inherited variants in candidate genes involved in oestrogen signalling and tamoxifen metabolism might be associated with tamoxifen effects in bone...
  18. pmc Aromatase inhibitor-induced modulation of breast density: clinical and genetic effects
    N L Henry
    Breast Oncology Program, University of Michigan Comprehensive Cancer Center, 1500 East Medical Center Drive, Med Inn Building C450, Ann Arbor, MI 48109 5843, USA
    Br J Cancer 109:2331-9. 2013
    ..We performed a prospective clinical trial to evaluate the impact of inherited variants in genes involved in oestrogen metabolism and signalling on change in mammographic percent density (MPD) with aromatase inhibitor (AI) therapy...
  19. doi request reprint Correlation of GREB1 mRNA with protein expression in breast cancer: validation of a novel GREB1 monoclonal antibody
    H J Hnatyszyn
    Department of Medicine, Miller School of Medicine, University of Miami, Miami, FL, USA
    Breast Cancer Res Treat 122:371-80. 2010
    ..The novel monoclonal GREB1ab is specific for GREB1 protein. This antibody will serve as a tool for investigations focused on the expression, distribution, and function of GREB1 in normal breast and breast cancer tissues...
  20. pmc Efavirenz directly modulates the oestrogen receptor and induces breast cancer cell growth
    M J Sikora
    Department of Pharmacology, University of Michigan Medical Center, Ann Arbor, MI, USA
    HIV Med 11:603-7. 2010
    ..Efavirenz-based HIV therapy is associated with breast hypertrophy and gynaecomastia. Here, we tested the hypothesis that efavirenz induces gynaecomastia through direct binding and modulation of the oestrogen receptor (ER)...
  21. ncbi request reprint Common origins of MDA-MB-435 cells from various sources with those shown to have melanoma properties
    James M Rae
    Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA
    Clin Exp Metastasis 21:543-52. 2004
    ..Collectively, our results show that the MDA-MB-435 cells used widely have identical origins to those that exhibit a melanoma-like gene expression signature, but exhibit a small degree of genotypic and phenotypic drift...
  22. ncbi request reprint Pharmacogenetics of tamoxifen biotransformation is associated with clinical outcomes of efficacy and hot flashes
    Matthew P Goetz
    Department of Oncology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    J Clin Oncol 23:9312-8. 2005
    ..Polymorphisms in tamoxifen metabolizing genes affect the plasma concentration of tamoxifen metabolites, but their effect on clinical outcome is unknown...
  23. ncbi request reprint CYP2D6 genotype, antidepressant use, and tamoxifen metabolism during adjuvant breast cancer treatment
    Yan Jin
    Division of Clinical Pharmacology, Department of Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    J Natl Cancer Inst 97:30-9. 2005
    ....
  24. ncbi request reprint Active tamoxifen metabolite plasma concentrations after coadministration of tamoxifen and the selective serotonin reuptake inhibitor paroxetine
    Vered Stearns
    The Breast Cancer Program, Department of Medicine, Lombardi Cancer Center, Georgetown University Medical Center, Washington, DC, USA
    J Natl Cancer Inst 95:1758-64. 2003
    ..In a prospective clinical trial, we tested the effects of coadministration of tamoxifen and the SSRI paroxetine, an inhibitor of CYP2D6, on tamoxifen metabolism...
  25. pmc High-efficiency genotype analysis from formalin-fixed, paraffin-embedded tumor tissues
    M J Sikora
    Department of Pharmacology, University of Michigan Medical Center, Ann Arbor, MI 48109, USA
    Pharmacogenomics J 11:348-58. 2011
    ..The use of tumor cores is of particular importance as the harvesting of tumor cores has minimal impact on the utility of the donor blocks for other purposes...
  26. pmc Genes regulated by estrogen in breast tumor cells in vitro are similarly regulated in vivo in tumor xenografts and human breast tumors
    Chad J Creighton
    Bioinformatics Program, University of Michigan Medical Center, Ann Arbor, MI 48109, USA
    Genome Biol 7:R28. 2006
    ....
  27. pmc An expression signature of estrogen-regulated genes predicts disease-free survival in tamoxifen-treated patients better than progesterone receptor status
    Marc E Lippman
    Division of Hematology Oncology, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109, USA
    Trans Am Clin Climatol Assoc 119:77-90; discussion 90-2. 2008
    ..This combined biological and informatic analysis is potentially applicable to many other cancer therapeutics...
  28. pmc The androgen metabolite 5alpha-androstane-3beta,17beta-diol (3betaAdiol) induces breast cancer growth via estrogen receptor: implications for aromatase inhibitor resistance
    Matthew J Sikora
    Department of Pharmacology, University of Michigan Medical Center, Ann Arbor, MI 48109, USA
    Breast Cancer Res Treat 115:289-96. 2009
    ..The generation of estrogen-like steroids represents a potential mechanism of resistance to aromatase inhibitors...
  29. pmc Association between CYP2D6 genotype and tamoxifen-induced hot flashes in a prospective cohort
    N Lynn Henry
    Breast Oncology Program, University of Michigan Comprehensive Cancer Center, Ann Arbor, MI, USA
    Breast Cancer Res Treat 117:571-5. 2009
    ..062). CYP2D6 activity may be a modest predictive factor for tamoxifen-induced hot flashes. The presence or absence of hot flashes should not be used to determine tamoxifen's efficacy...
  30. ncbi request reprint Metabolism of N,N',N"-triethylenethiophosphoramide by CYP2B1 and CYP2B6 results in the inactivation of both isoforms by two distinct mechanisms
    Erin Harleton
    Department of Pharmacology, Medical Science Research Building III, 1150 West Medical Center Dr, Ann Arbor, MI 48109 0632, USA
    J Pharmacol Exp Ther 310:1011-9. 2004
    ..The data indicate that tTEPA metabolism by these two 2B isoforms results in inactivation of the P450s by two distinct mechanisms...
  31. ncbi request reprint The impact of cytochrome P450 2D6 metabolism in women receiving adjuvant tamoxifen
    Matthew P Goetz
    Department of Oncology, Mayo Clinic College of Medicine, 200 First Street Southwest, Rochester, MN, 55905, USA
    Breast Cancer Res Treat 101:113-21. 2007
    ....
  32. pmc Monitoring serial changes in circulating human breast cancer cells in murine xenograft models
    Jean Pierre Eliane
    Breast Oncology Program, Comprehensive Cancer Center, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109 0942, USA
    Cancer Res 68:5529-32. 2008
    ..These results establish a new method for studying CTC in mouse models of epithelial cancer, providing the foundation for studies of molecular regulation of CTC in cancer and CTC as biomarker for therapeutic efficacy...
  33. pmc Expression levels and activation of a PXR variant are directly related to drug resistance in osteosarcoma cell lines
    Edith J Mensah-Osman
    Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA
    Cancer 109:957-65. 2007
    ....
  34. ncbi request reprint Combination vinorelbine and capecitabine for metastatic breast cancer using a non-body surface area dosing scheme
    Anne F Schott
    Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, USA
    Cancer Chemother Pharmacol 58:129-35. 2006
    ..A flat dosing scheme of both drugs was used to facilitate development of the oral regimen, and because neither drug's clearance is associated with body surface area (BSA), pharmacokinetic and pharmacogenetic endpoints were explored...
  35. pmc Reliable gene expression measurements from fine needle aspirates of pancreatic tumors: effect of amplicon length and quality assessment
    Michelle A Anderson
    Department of Internal Medicine, Division of Gastroenterology, University of Michigan Health System, Ann Arbor, Michigan 48109 0362, USA
    J Mol Diagn 12:566-75. 2010
    ..Fine needle aspirates (FNAs) from pancreatic masses were studied to define potential causes of RNA degradation and develop methods for accurately measuring gene expression...
  36. ncbi request reprint Rifampin is a selective, pleiotropic inducer of drug metabolism genes in human hepatocytes: studies with cDNA and oligonucleotide expression arrays
    J M Rae
    Department of Pharmacology, Georgetown University Medical Center, Washington, DC, USA
    J Pharmacol Exp Ther 299:849-57. 2001
    ..Clinicians and researchers who use and study rifampin and other drugs that induce drug metabolism should be alert to the possibility of multiple effects...
  37. pmc Cytochrome P450 2D6 and homeobox 13/interleukin-17B receptor: combining inherited and tumor gene markers for prediction of tamoxifen resistance
    Matthew P Goetz
    Department of Oncology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    Clin Cancer Res 14:5864-8. 2008
    ..We sought to determine the combined effect of inherited (CYP2D6) and somatic (HOXB13/IL17BR) gene variation in tamoxifen-treated breast cancer...
  38. ncbi request reprint Activation of mitogen-activated protein kinase in estrogen receptor alpha-positive breast cancer cells in vitro induces an in vivo molecular phenotype of estrogen receptor alpha-negative human breast tumors
    Chad J Creighton
    Bioinformatics Program, University of Michigan Medical Center, 1150 West Medical Center Drive, Ann Arbor, MI 48109, USA
    Cancer Res 66:3903-11. 2006
    ..These MAPK+ cell lines are excellent models for investigating the underlying mechanisms behind the ERalpha- phenotype...
  39. ncbi request reprint Cytochrome P450 3A pharmacogenetics: the road that needs traveled
    D A Flockhart
    Division of Clinical Pharmacology, Indiana University School of Medicine, Indianapolis 46202, USA
    Pharmacogenomics J 3:3-5. 2003
  40. pmc The endocannabinoid anandamide is a substrate for the human polymorphic cytochrome P450 2D6
    Natasha T Snider
    Department of Pharmacology, University of Michigan, Ann Arbor, MI 48109 5632, USA
    J Pharmacol Exp Ther 327:538-45. 2008
    ..This study also offers support to the hypothesis that neuropsychiatric phenotype differences among individuals with genetic variations in CYP2D6 could be ascribable to interactions of this enzyme with endogenous substrates...
  41. ncbi request reprint (-)-Gossypol enhances response to radiation therapy and results in tumor regression of human prostate cancer
    Liang Xu
    Department of Internal Medicine, Division of Hematology and Oncology, University of Michigan, 5301B Medical Science Research Building III, 1500 West Medical Center Drive, Ann Arbor, MI 48109 0640, USA
    Mol Cancer Ther 4:197-205. 2005
    ..Gossypol may improve the outcome of current prostate cancer radiotherapy and represents a promising novel anticancer regime for molecular targeted therapy of hormone-refractory prostate cancer with Bcl-2/Bcl-xL overexpression...
  42. ncbi request reprint LCC15-MB cells are MDA-MB-435: a review of misidentified breast and prostate cell lines
    Erik W Thompson
    Invasion and Metastasis Unit, University of Melbourne, Department of Surgery, St Vincent s Hospital, 29 Regent St, Fitzroy, 3065, Australia
    Clin Exp Metastasis 21:535-41. 2004
    ..We also review the known misclassification of breast and prostate cancer cell lines to date and have initiated a register maintained at http://www.svi.edu.au/cell_lines_registry.doc...
  43. pmc A transcriptional fingerprint of estrogen in human breast cancer predicts patient survival
    Jianjun Yu
    Bioinformatigram, The University of of Michigan Medical Center, Ann Arbor, MI 48109 USA
    Neoplasia 10:79-88. 2008
    ..Taken together, these data demonstrate the power of using cell culture systems to screen for robust gene signatures of clinical relevance...
  44. ncbi request reprint Sequence diversity and functional characterization of the 5'-regulatory region of human CYP2C19
    Million Arefayene
    Department of Medicine Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    Pharmacogenetics 13:199-206. 2003
    ..These data make possible future studies to elucidate the molecular mechanisms by which CYP2C19 can be induced in clinical settings and the consequences of genetic variability in its promoter...
  45. ncbi request reprint Association of genetic variation in tamoxifen-metabolizing enzymes with overall survival and recurrence of disease in breast cancer patients
    Susan A Nowell
    Department of Epidemiology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
    Breast Cancer Res Treat 91:249-58. 2005
    ..These studies indicate that genetic variation in Phase II conjugating enzymes can influence the efficacy of tamoxifen therapy for breast cancer...