Matthew Raab

Summary

Affiliation: University of Pennsylvania
Country: USA

Publications

  1. ncbi request reprint Polymersomes
    Dennis E Discher
    Biophysical and Polymers Engineering Lab, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Annu Rev Biomed Eng 8:323-41. 2006
  2. pmc Matrix elasticity in vitro controls muscle stem cell fate in vivo
    Matthew Raab
    University of Pennsylvania, 129 Towne Bldg, Philadelphia, PA, USA 19104
    Stem Cell Res Ther 1:38. 2010
  3. ncbi request reprint Polymer vesicles
    Dennis E Discher
    School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA 19104 6393, USA
    Science 297:967-73. 2002
  4. pmc Covalent chemistry on distended proteins
    Dennis E Discher
    Molecular and Cell Biophysics Laboratory and Nano Bio Interface Center, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 103:7533-4. 2006
  5. ncbi request reprint Tissue cells feel and respond to the stiffness of their substrate
    Dennis E Discher
    School of Engineering and Applied Science and Cell and Molecular Biology Graduate Group, University of Pennsylvania, Philadelphia, PA 19104 6315, USA
    Science 310:1139-43. 2005
  6. pmc Biomechanics: cell research and applications for the next decade
    Dennis Discher
    University of Pennsylvania, Philadelphia, PA, USA
    Ann Biomed Eng 37:847-59. 2009
  7. pmc Growth factors, matrices, and forces combine and control stem cells
    Dennis E Discher
    Biophysical Engineering and Nanobiopolymers Laboratory, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA 19104, USA
    Science 324:1673-7. 2009
  8. doi request reprint The Foldome in cellular force transduction
    Dennis E Discher
    University of Pennsylvania, USA
    Conf Proc IEEE Eng Med Biol Soc 2009:3341-2. 2009
  9. pmc Self inhibition of phagocytosis: the affinity of 'marker of self' CD47 for SIRPalpha dictates potency of inhibition but only at low expression levels
    Richard K Tsai
    Biophysical Engineering Lab, University of Pennsylvania, Philadelphia, PA 19104, USA
    Blood Cells Mol Dis 45:67-74. 2010
  10. doi request reprint Hyaluronic acid matrices show matrix stiffness in 2D and 3D dictates cytoskeletal order and myosin-II phosphorylation within stem cells
    Florian Rehfeldt
    Biophysical Eng g Lab, University of Pennsylvania, Philadelphia, PA 19104, USA
    Integr Biol (Camb) 4:422-30. 2012

Collaborators

Detail Information

Publications77

  1. ncbi request reprint Polymersomes
    Dennis E Discher
    Biophysical and Polymers Engineering Lab, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Annu Rev Biomed Eng 8:323-41. 2006
    ..Comparisons of polymersomes with viral capsids are shown to encompass and inspire many aspects of current designs...
  2. pmc Matrix elasticity in vitro controls muscle stem cell fate in vivo
    Matthew Raab
    University of Pennsylvania, 129 Towne Bldg, Philadelphia, PA, USA 19104
    Stem Cell Res Ther 1:38. 2010
    ..One recent study even shows that muscle stem cells expand much better in vitro on muscle-mimetic gels and that such cells prove optimal for engraftment in muscle...
  3. ncbi request reprint Polymer vesicles
    Dennis E Discher
    School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA 19104 6393, USA
    Science 297:967-73. 2002
    ....
  4. pmc Covalent chemistry on distended proteins
    Dennis E Discher
    Molecular and Cell Biophysics Laboratory and Nano Bio Interface Center, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 103:7533-4. 2006
  5. ncbi request reprint Tissue cells feel and respond to the stiffness of their substrate
    Dennis E Discher
    School of Engineering and Applied Science and Cell and Molecular Biology Graduate Group, University of Pennsylvania, Philadelphia, PA 19104 6315, USA
    Science 310:1139-43. 2005
    ..The feedback of local matrix stiffness on cell state likely has important implications for development, differentiation, disease, and regeneration...
  6. pmc Biomechanics: cell research and applications for the next decade
    Dennis Discher
    University of Pennsylvania, Philadelphia, PA, USA
    Ann Biomed Eng 37:847-59. 2009
    ..Such insights will no doubt inform basic biology and rational engineering of effective new approaches to clinical therapy...
  7. pmc Growth factors, matrices, and forces combine and control stem cells
    Dennis E Discher
    Biophysical Engineering and Nanobiopolymers Laboratory, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA 19104, USA
    Science 324:1673-7. 2009
    ..Multifaceted technologies are increasingly required to produce and interrogate cells ex vivo, to build predictive models, and, ultimately, to enhance stem cell integration in vivo for therapeutic benefit...
  8. doi request reprint The Foldome in cellular force transduction
    Dennis E Discher
    University of Pennsylvania, USA
    Conf Proc IEEE Eng Med Biol Soc 2009:3341-2. 2009
    ....
  9. pmc Self inhibition of phagocytosis: the affinity of 'marker of self' CD47 for SIRPalpha dictates potency of inhibition but only at low expression levels
    Richard K Tsai
    Biophysical Engineering Lab, University of Pennsylvania, Philadelphia, PA 19104, USA
    Blood Cells Mol Dis 45:67-74. 2010
    ..Quantitative analyses here prompt more complete characterizations of both CD47 levels and SIRPalpha polymorphisms when attempting to study in vivo effects of these key proteins in innate immunity...
  10. doi request reprint Hyaluronic acid matrices show matrix stiffness in 2D and 3D dictates cytoskeletal order and myosin-II phosphorylation within stem cells
    Florian Rehfeldt
    Biophysical Eng g Lab, University of Pennsylvania, Philadelphia, PA 19104, USA
    Integr Biol (Camb) 4:422-30. 2012
    ..The results can be understood in part from a theory for stress fiber polarization that couples to matrix elasticity as well as cell shape and accurately predicts cytoskeletal order in 2D and 3D, regardless of polymer system...
  11. pmc Indentation and adhesive probing of a cell membrane with AFM: theoretical model and experiments
    Shamik Sen
    Biophysical Engineering Lab, University of Pennsylvania, Philadelphia, PA, USA
    Biophys J 89:3203-13. 2005
    ..Membrane mechanical properties thus clearly influence AFM probing of cells, including single molecule adhesion experiments...
  12. pmc Crawling from soft to stiff matrix polarizes the cytoskeleton and phosphoregulates myosin-II heavy chain
    Matthew Raab
    Molecular and Cell Biophysics Laboratory, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Cell Biol 199:669-83. 2012
    ..Polarization is thus shown to be a highly regulated compass for mechanosensitive migration...
  13. pmc Forced unfolding of proteins within cells
    Colin P Johnson
    Biophysical Engineering Lab, University of Pennsylvania, Philadelphia, PA 19104, USA
    Science 317:663-6. 2007
    ....
  14. pmc Inhibition of "self" engulfment through deactivation of myosin-II at the phagocytic synapse between human cells
    Richard K Tsai
    Biophysical Engineering Laboratory, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Cell Biol 180:989-1003. 2008
    ..A point mutation turns off this motor's contribution to phagocytosis, suggesting that self-recognition inhibits contractile engulfment...
  15. ncbi request reprint Cell responses to the mechanochemical microenvironment--implications for regenerative medicine and drug delivery
    Florian Rehfeldt
    Department of Physics and Astronomy, University of Pennsylvania, Philadelphia PA 19104, USA
    Adv Drug Deliv Rev 59:1329-39. 2007
    ..The differential effects not only lend insight into mechano-sensing of the substrate by cells, but also have important implications for regeneration and molecular therapies...
  16. pmc Cooperativity in forced unfolding of tandem spectrin repeats
    Richard Law
    Biophysical Engineering Lab, Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia 19104, USA
    Biophys J 84:533-44. 2003
    ..Cooperativity in forced unfolding of spectrin is also clearly demonstrated by a common force scale for the unfolding of both single and tandem repeats...
  17. ncbi request reprint Self-assembly and properties of diblock copolymers by coarse-grain molecular dynamics
    Goundla Srinivas
    Center for Molecular Modeling, Department of Chemistry, University of Pennsylvania, Philadelphia 19104 6323, USA
    Nat Mater 3:638-44. 2004
    ..Calculated trends with MW as well as hydrophilic/hydrophobic ratio thus agree with experiment, demonstrating that CG-MD simulations provide a rational design tool for diblock copolymer assemblies...
  18. ncbi request reprint Key roles for chain flexibility in block copolymer membranes that contain pores or make tubes
    Goundla Srinivas
    Center for Molecular Modeling, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    Nano Lett 5:2343-9. 2005
    ..A-B-A triblocks that exploit "hairpin" and "straight" conformations also show assembly into novel nanotubules and further highlight the key roles for chain flexibility in biomimetic block copolymer assemblies...
  19. ncbi request reprint Electric field manipulation of charged copolymer worm micelles
    Kandaswamy Vijayan
    Graduate Group in Physics, Chemical and Biomolecular Engineering, and The Laboratory for Research on the Structure of Matter, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Phys Chem B 110:3831-4. 2006
    ..A brief discussion on using this technique to estimate the rheological properties of wormlike micelles is presented...
  20. pmc Polymersome carriers: from self-assembly to siRNA and protein therapeutics
    David A Christian
    Biophysical Engineering and NanoBio Polymers Lab, University of Pennsylvania, Philadelphia, PA 19104, USA
    Eur J Pharm Biopharm 71:463-74. 2009
    ..Here we summarize the assembly and development of controlled release polymersomes to encapsulate therapeutics ranging from small molecule anti-cancer drugs to siRNA and therapeutic proteins...
  21. ncbi request reprint A hemoglobin fragment found in cervicovaginal fluid from women in labor potentiates the action of agents that promote contraction of smooth muscle cells
    Amy G Brown
    Center for Research on Reproduction and Women s Health, University of Pennsylvania Medical School, Biomedical Research Building II III, 421 Curie Blvd, Philadelphia, PA 19104, USA
    Peptides 27:1794-800. 2006
    ..This peptide may promote vasoconstriction and augment normal labor through enhancing the action of uterotonins...
  22. ncbi request reprint Domain unfolding in neurofilament sidearms: effects of phosphorylation and ATP
    Helim Aranda-Espinoza
    Institute for Medicine and Engineering, 1080 Vagelos Research Laboratory, 3340 Smith Walk, University of Pennsylvania, Philadelphia, PA 19104, USA
    FEBS Lett 531:397-401. 2002
    ..However, the native phosphorylated SA requires three-fold higher force to unfold by AFM than dephosphorylated SA, suggesting a less pliant as well as larger structure when phosphorylated...
  23. ncbi request reprint Dissipative particle dynamics simulations of polymersomes
    Vanessa Ortiz
    Max Planck Institute of Colloids and Interfaces Golm, D 14424 Potsdam, Germany
    J Phys Chem B 109:17708-14. 2005
    ..The results should provide guidance for theoretical derivations of scaling laws and also illustrate how spherical polymer vesicles might be studied in simulation...
  24. pmc Stem cells, microenvironment mechanics, and growth factor activation
    Rebeca M Tenney
    Cell and Molecular Biology Graduate Group, University of Pennsylvania, Philadelphia, PA 19104, USA
    Curr Opin Cell Biol 21:630-5. 2009
    ..We discuss these processes and pathways in the contexts of matrix adhesion and fluid shearing as they might relate to stem cell differentiation and other mechanisms in development, disease, and repair...
  25. pmc Pathway shifts and thermal softening in temperature-coupled forced unfolding of spectrin domains
    Richard Law
    Biophysical Engineering Lab, Institute for Medicine and Engineering, and School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6315, USA
    Biophys J 85:3286-93. 2003
    ..In sum, structural changes with temperature correlate with both single-molecule unfolding forces and shifts in unfolding pathways...
  26. ncbi request reprint Polymer vesicles in vivo: correlations with PEG molecular weight
    Peter J Photos
    Department of Chemical and Biomolecular Engineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA 19104 6393, USA
    J Control Release 90:323-34. 2003
    ..The stealthiness introduced to liposomes through PEGylation is thus extended here with completely synthetic polymersomes...
  27. doi request reprint Cell differentiation through tissue elasticity-coupled, myosin-driven remodeling
    Allison L Zajac
    Biophysical Engineering Lab, and Cell and Molecular Biology Graduate Group, University of Pennsylvania, Philadelphia, PA, USA
    Curr Opin Cell Biol 20:609-15. 2008
    ..We then highlight what is known of molecular remodeling in cells under stress on short time scales. Key roles for forces generated by ubiquitous and essential myosin-II motors in feedback remodeling are emphasized throughout...
  28. ncbi request reprint Effect of polymer amphiphilicity on loading of a therapeutic enzyme into protective filamentous and spherical polymer nanocarriers
    ERIC A SIMONE
    Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Biomacromolecules 8:3914-21. 2007
    ..PNC morphology also greatly influenced its degradation, offering a new means of controlled delivery...
  29. pmc Matrix elasticity, cytoskeletal tension, and TGF-beta: the insoluble and soluble meet
    Rebecca G Wells
    Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Sci Signal 1:pe13. 2008
    ..More broadly, the work suggests that matrix stiffness could regulate the equilibrium between storage and release of a host of matrix-bound growth factors...
  30. ncbi request reprint Matrix elasticity directs stem cell lineage specification
    Adam J Engler
    Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, 19104, USA
    Cell 126:677-89. 2006
    ..The results have significant implications for understanding physical effects of the in vivo microenvironment and also for therapeutic uses of stem cells...
  31. pmc Conformational changes and signaling in cell and matrix physics
    Andre E X Brown
    Department of Physics and Astronomy, University of Pennsylvania, Philadelphia, PA 19104, USA
    Curr Biol 19:R781-9. 2009
    ....
  32. ncbi request reprint Microtissue elasticity: measurements by atomic force microscopy and its influence on cell differentiation
    Adam J Engler
    Biophysical Engineering and Polymers Laboratory, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Methods Cell Biol 83:521-45. 2007
    ..The approach is briefly illustrated with lineage commitment of stem cells due to matrix elasticity...
  33. pmc Filamentous polymer nanocarriers of tunable stiffness that encapsulate the therapeutic enzyme catalase
    ERIC A SIMONE
    Department of Bioengineering, Institute for Translational Medicine and Therapeutics, and Institute for Environmental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Biomacromolecules 10:1324-30. 2009
    ..Thus, we report here for the first time f-PNC that encapsulate an active enzyme with polymer MW-tunable flexibility, offering several potential therapeutic applications...
  34. pmc Spotted vesicles, striped micelles and Janus assemblies induced by ligand binding
    David A Christian
    Laboratory for Research on the Structure of Matter, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Nat Mater 8:843-9. 2009
    ....
  35. pmc Preparation of collagen-coated gels that maximize in vitro myogenesis of stem cells by matching the lateral elasticity of in vivo muscle
    Tathagata Chaudhuri
    Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, PA, USA
    Methods Mol Biol 621:185-202. 2010
    ....
  36. ncbi request reprint Protein 4.2 is critical to CD47-membrane skeleton attachment in human red cells
    Kris Noel Dahl
    Department of Chemical and Biomolecular Engineering and School of Engineering and Applied Science, Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA 19104, USA
    Blood 103:1131-6. 2004
    ..2 strongly influences CD47 levels as well as the extent of membrane skeleton attachment in the RBC, whereas protein 4.2 affects membrane skeletal attachment of RhAG, Rh, and band 3 to a lesser extent...
  37. ncbi request reprint Adhesion-contractile balance in myocyte differentiation
    Maureen A Griffin
    Biophysical Engineering Laboratory, 112 Towne Building, and Pennsylvania Muscle Institute, University of Pennsylvania, D 700 Richards Building, 3700 Hamilton Walk, Philadelphia, PA 19104, USA
    J Cell Sci 117:5855-63. 2004
    ..Myotubes in culture are thus clearly prestressed by myosin II, and this contractility couples to substrate compliance and ultimately influences actomyosin striation...
  38. pmc Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments
    Adam J Engler
    School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Cell Biol 166:877-87. 2004
    ..These findings have major implications for in vivo introduction of stem cells into diseased or damaged striated muscle of altered mechanical composition...
  39. ncbi request reprint Targeted worm micelles
    Paul Dalhaimer
    Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia 19104 6391, USA
    Biomacromolecules 5:1714-9. 2004
    ..The results thus demonstrate the feasibility of targeted delivery by polymeric worm micelles...
  40. pmc Species- and cell type-specific interactions between CD47 and human SIRPalpha
    Shyamsundar Subramanian
    School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA, USA
    Blood 107:2548-56. 2006
    ..The results thus demonstrate that SIRPalpha-CD47 interactions, which reportedly define self, exhibit cell type specificity and limited cross-species reactivity...
  41. pmc Patterning, prestress, and peeling dynamics of myocytes
    Maureen A Griffin
    Department of Chemical and Biomolecular Engineering, Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Biophys J 86:1209-22. 2004
    ....
  42. pmc Embryonic cardiomyocytes beat best on a matrix with heart-like elasticity: scar-like rigidity inhibits beating
    Adam J Engler
    Molecular and Cell Biophysics Lab, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Cell Sci 121:3794-802. 2008
    ..The results described here also highlight the need for greater attention to fibrosis and mechanical microenvironments in cell therapy and development...
  43. pmc Substrate compliance versus ligand density in cell on gel responses
    Adam Engler
    School of Engineering and Applied Science, Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Biophys J 86:617-28. 2004
    ..Based on such results, we hypothesize a central structural role for the cytoskeleton in driving the membrane outward during spreading whereas adhesion reinforces the spreading...
  44. pmc Matrix elasticity, cytoskeletal forces and physics of the nucleus: how deeply do cells 'feel' outside and in?
    Amnon Buxboim
    Biophysical Engineering Lab, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Cell Sci 123:297-308. 2010
    ..Ultimately, cells feel the difference between stiff or soft and thick or thin surroundings, regardless of whether or not they are of royal descent...
  45. pmc Shape effects of filaments versus spherical particles in flow and drug delivery
    Yan Geng
    NanoBioPolymers and Molecular and Cell Biophysics Lab, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Nat Nanotechnol 2:249-55. 2007
    ..Although these findings show that long-circulating vehicles need not be nanospheres, they also lend insight into possible shape effects of natural filamentous viruses...
  46. ncbi request reprint Efficient nuclear delivery and nuclear body localization of antisense oligo-nucleotides using degradable polymersomes
    Younghoon Kim
    Chem and Biomolecular Eng Dept, Pennsylvania Univ, Philadelphia, PA 19104, USA
    Conf Proc IEEE Eng Med Biol Soc 1:4350-3. 2006
    ..In sum, these neutral, degradable carriers of AON show promise in vivo...
  47. pmc Flexible filaments for in vivo imaging and delivery: persistent circulation of filomicelles opens the dosage window for sustained tumor shrinkage
    David A Christian
    Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Mol Pharm 6:1343-52. 2009
    ..These results thus begin to address mechanisms for how nonspherical carriers deliver both imaging agents and anticancer therapeutics to solid tumors...
  48. pmc Cross-correlated TIRF/AFM reveals asymmetric distribution of force-generating heads along self-assembled, "synthetic" myosin filaments
    Andre E X Brown
    Department of Physics and Astronomy, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Biophys J 96:1952-60. 2009
    ..Such strongly asymmetric bipoles are likely to produce an unbalanced contractile force in cells and in actin-myosin gels and thereby contribute to motility as well as cytoskeletal tension...
  49. pmc Adhesively-tensed cell membranes: lysis kinetics and atomic force microscopy probing
    Alina Hategan
    Biophysical Engineering Lab and Institute for Medicine and Engineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6315, USA
    Biophys J 85:2746-59. 2003
    ..In sum we show how adhesive tensioning of the red cell can be used to gain novel insights into native membrane dynamics and structure...
  50. ncbi request reprint Molecular extensibility of mini-dystrophins and a dystrophin rod construct
    Nishant Bhasin
    Pennsylvania Muscle Institute and Graduate Groups in Physics and Cell and Molecular Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Mol Biol 352:795-806. 2005
    ..The results thus reveal new modes of dystrophin flexibility that may prove central to functions of both dystrophin and mini-dystrophins...
  51. ncbi request reprint Phylogenetic divergence of CD47 interactions with human signal regulatory protein alpha reveals locus of species specificity. Implications for the binding site
    Shyamsundar Subramanian
    Biophysical Engineering Laboratory, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 282:1805-18. 2007
    ..Direct tests of cell-cell adhesion between human monocytes and CD47-displaying CHO cells affirm the species specificity as well as the importance of the newly identified binding locus in cell-cell interactions...
  52. ncbi request reprint Visualizing worm micelle dynamics and phase transitions of a charged diblock copolymer in water
    Yan Geng
    Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6391, USA
    J Phys Chem B 109:3772-9. 2005
    ..Both the transition kinetics and phase diagrams indicate divalent cation is about 5-10-fold more effective than monovalent in stabilizing all nonspherical morphologies...
  53. pmc Forced unfolding of coiled-coils in fibrinogen by single-molecule AFM
    Andre E X Brown
    Department of Physics and Astronomy, Nano Bio Interface Center, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Biophys J 92:L39-41. 2007
    ..The distinct behavior of fibrinogen seems to be due to its topologically complex coiled-coils and an interaction between fibrinogen's alphaC-domains and its central region...
  54. ncbi request reprint Shrinkage of a rapidly growing tumor by drug-loaded polymersomes: pH-triggered release through copolymer degradation
    Fariyal Ahmed
    Chemical and Biomolecular Engineering, Center for Molecular Modeling, Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Mol Pharm 3:340-50. 2006
    ..Neutral polymersomes and related macrosurfactant assemblies can thus create novel pathways within cells for controlled release and delivery...
  55. ncbi request reprint Biodegradable polymersomes loaded with both paclitaxel and doxorubicin permeate and shrink tumors, inducing apoptosis in proportion to accumulated drug
    Fariyal Ahmed
    Departments of Chemical Biomolecular Engineering and Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Control Release 116:150-8. 2006
    ..The polymersomes cause two-fold higher cell death in tumors than free drug and show quantitatively similar increases in maximum tolerated dose and drug accumulation within the tumors-suggesting promise for multi-drug delivery...
  56. ncbi request reprint Mesenchymal stem cell injection after myocardial infarction improves myocardial compliance
    Mark F Berry
    Division of Cardiothoracic Surgery, Department of Physiology, University of Pennsylvania School of Medicine, A700 Richards Bldg, 3700 Hamilton Walk, Philadelphia, PA 19104 6085, USA
    Am J Physiol Heart Circ Physiol 290:H2196-203. 2006
    ..Improving scarred heart muscle compliance could be a functional benefit of cellular cardiomyoplasty...
  57. ncbi request reprint Self-porating polymersomes of PEG-PLA and PEG-PCL: hydrolysis-triggered controlled release vesicles
    Fariyal Ahmed
    Department of Chemical Biomolecular Engineering, University of Pennsylvania, 112 Towne Building, Philadelphia, PA 19104, USA
    J Control Release 96:37-53. 2004
    ..Kinetics of this evolving detergent mechanism overlay the phase behavior of amphiphiles with transitions from membranes to micelles allowing controlled release...
  58. ncbi request reprint Micelles of different morphologies--advantages of worm-like filomicelles of PEO-PCL in paclitaxel delivery
    Shenshen Cai
    Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Pharm Res 24:2099-109. 2007
    ..5 kDa]) in order to compare loading and delivery of hydrophobic drugs...
  59. pmc Topographical pattern dynamics in passive adhesion of cell membranes
    Alina Hategan
    Biophysical Engineering Laboratory, Department of Chemical and Biomolecular Engineering and Graduate Group in Physics, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6315, USA
    Biophys J 87:3547-60. 2004
    ....
  60. ncbi request reprint Influence of lateral association on forced unfolding of antiparallel spectrin heterodimers
    Richard Law
    Department of Chemical and Biomolecular Engineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6315, USA
    J Biol Chem 279:16410-6. 2004
    ..Weak lateral interactions do not inhibit unfolding, but strong lateral interactions facilitate simultaneous unfolding analogous to serial repeat coupling within spectrin family proteins...
  61. pmc Calcium-dependent lateral organization in phosphatidylinositol 4,5-bisphosphate (PIP2)- and cholesterol-containing monolayers
    Ilya Levental
    Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Biochemistry 48:8241-8. 2009
    ..This result emphasizes the likely critical role of subphase factors and lipid head-group specificity in the formation and stability of cholesterol-dependent domains in cellular plasma membranes...
  62. pmc Hydrolytic degradation of poly(ethylene oxide)-block-polycaprolactone worm micelles
    Yan Geng
    Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Am Chem Soc 127:12780-1. 2005
    ..Such worm micelles spontaneously shorten to generate spherical micelles, triggered by polycaprolactone hydrolysis, with distinct mechanism and kinetics from that which occurs in bulk material...
  63. pmc Stem cells feel the difference
    Amnon Buxboim
    University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Nat Methods 7:695-7. 2010
    ..Arrays of microposts of different heights generate substrates with different flexibility, on which cells can be grown...
  64. ncbi request reprint The nuclear envelope lamina network has elasticity and a compressibility limit suggestive of a molecular shock absorber
    Kris Noel Dahl
    Department of Chemical and Biomolecular Engineering, 220 South 33rd Street, University of Pennsylvania, Philadelphia, PA 19104 6393, USA
    J Cell Sci 117:4779-86. 2004
    ....
  65. pmc Physical plasticity of the nucleus in stem cell differentiation
    J David Pajerowski
    Molecular and Cell Biophysics Laboratory, 129 Towne Building, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 104:15619-24. 2007
    ..The rheological character of the nucleus is thus set largely by nucleoplasm/chromatin, whereas the extent of deformation is modulated by the lamina...
  66. pmc Polymersome delivery of siRNA and antisense oligonucleotides
    Younghoon Kim
    Department of Chemical and Biomolecular Engineering and Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, USA
    J Control Release 134:132-40. 2009
    ..The lack of cationic groups in antisense polymersomes together with initial tests of efficacy suggests broader utility of these non-viral carriers...
  67. ncbi request reprint Unfolding a linker between helical repeats
    Vanessa Ortiz
    Center for Molecular Modeling, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Mol Biol 349:638-47. 2005
    ..The end result is an unfolded helix that mechanically decouples tandem repeats. Molecularly detailed insights obtained here aid in understanding the mechanical coupling of domain stability in spectrin family proteins...
  68. pmc Power-law rheology of isolated nuclei with deformation mapping of nuclear substructures
    Kris Noel Dahl
    Johns Hopkins University, Baltimore, MD 21202, USA
    Biophys J 89:2855-64. 2005
    ..Such results indicate an essentially infinite spectrum of timescales for structural reorganization, with implications for regulating genome expression kinetics...
  69. ncbi request reprint Self-assembly: towards precision micelles
    Dennis E Discher
    Nature 430:519-20. 2004
  70. ncbi request reprint Grafting short peptides onto polybutadiene-block-poly(ethylene oxide): a platform for self-assembling hybrid amphiphiles
    Yan Geng
    Department of Chemistry, University of Georgia, Athens, GA 30602, USA
    Angew Chem Int Ed Engl 45:7578-81. 2006
  71. ncbi request reprint Materials science: embedded shells decalcified
    Catherine Picart
    Nature 448:879-80. 2007
  72. pmc Interactions of membrane-active peptides with thick, neutral, nonzwitterionic bilayers
    Kandaswamy Vijayan
    Departments of Physics, of Chemical and Biomolecular Engineering, and of Physiology and Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Phys Chem B 109:14356-64. 2005
    ..We find that although alamethicin is too small to span the bilayer, the peptide interacts with, and ruptures, thick polymer membranes...
  73. ncbi request reprint gamma-Sarcoglycan deficiency increases cell contractility, apoptosis and MAPK pathway activation but does not affect adhesion
    Maureen A Griffin
    Pennsylvania Muscle Institute, University of Pennsylvania Medical Center, D 700 Richards Building, 3700 Hamilton Walk, Philadelphia, PA 19104 6083, USA
    J Cell Sci 118:1405-16. 2005
    ..We conclude that gammaSG normally moderates contractile prestress in skeletal muscle, and we propose a role for gammaSG in membrane-based signaling of the effects of prestress and sarcomerogenesis...
  74. ncbi request reprint Nuclear mechanics and methods
    Jan Lammerding
    Department of Medicine, Cardiovascular Division, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Methods Cell Biol 83:269-94. 2007
    ..Various methods to measure nuclear mechanics, isolated or in situ, are outlined here in some detail...
  75. ncbi request reprint Developmental biology: holding it together in the eye
    Paul A Janmey
    Nature 431:635-6. 2004
  76. ncbi request reprint Microscopic methods for measuring the elasticity of gel substrates for cell culture: microspheres, microindenters, and atomic force microscopy
    Margo T Frey
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Methods Cell Biol 83:47-65. 2007
    ..Here we describe three methods that have been applied successfully to probe the flexibility of soft culture substrates...
  77. ncbi request reprint Elasticity of native and cross-linked polyelectrolyte multilayer films
    Ludovic Richert
    Institut National de la Sante et de la Recherche Medicale, Unite 595, Universite Louis Pasteur, 11 rue Humann, 67085 Strasbourg Cedex, France
    Biomacromolecules 5:1908-16. 2004
    ..Spreading of smooth muscle cells on these substrates with pre-attached collagen proved to be highly dependent on film rigidity with stiffer films giving greater cell spreading...

Research Grants20

  1. AFM(T) + FLUORESCENCE MEASUREMENTS ON SINGLE MOLECULES
    Dennis Discher; Fiscal Year: 2007
    ....
  2. Carrier Shape Matters: Filomicelles, Long-circulation, and the EPR effect
    Dennis Discher; Fiscal Year: 2007
    ..We propose a focused development of therapeutic ligands, including targeted apoptosis-inducers, as we ultimately seek a wider range of control and targeting (but passive first!) of copolymer assemblies for in vivo studies. ..
  3. Carrier Shape Matters: Filomicelles, Long-circulation, and the EPR effect
    Dennis Discher; Fiscal Year: 2009
    ..We propose a focused development of therapeutic ligands, including targeted apoptosis-inducers, as we ultimately seek a wider range of control and targeting (but passive first!) of copolymer assemblies for in vivo studies. ..
  4. Cytoskeletal Pliability within Cells under Stress: RBCs and Marrow-derived SCs
    Dennis Discher; Fiscal Year: 2009
    ..New therapeutic strategies that could benefit more broadly include exon skipping strategies that are presently being applied to the spectrin family protein dystrophin [Goyenvalle Science 2006]. ..
  5. SPECTRIN EXTENSIBILITY and CD47 CONNECTIONS & FUNCTION
    Dennis Discher; Fiscal Year: 2007
    ..g., hereditary spherocytosis) and senescence, as well as fleshing out human-mouse comparisons on what is already emerging as a structurally divergent system. ..
  6. Myocytes sense substrate stiffness - New materials
    Dennis Discher; Fiscal Year: 2006
    ..PEM coating of stents will also be examined for inhibiting the proliferative response of smooth muscle cells implicated in restenosis. ..
  7. RED CELL SPECTRIN AND ACTIN MICROSTRUCTURAL RESPONSES
    Dennis Discher; Fiscal Year: 2002
    ..abstract_text> ..
  8. CD47-Display on Nanomaterials
    Dennis Discher; Fiscal Year: 2004
    ..abstract_text> ..
  9. Worm-like Micelles for Targeted Delivery and Imaging
    Dennis Discher; Fiscal Year: 2005
    ..Worm micelles may find a place in novel therapies. Regardless, worm micelles will teach us about biotransport, biocompatibility, and multi-valent targeting of long cylindrical objects both in the circulation and into cells. ..
  10. Cytoskeletal Pliability within Cells under Stress: RBCs and Marrow-derived SCs
    Dennis E Discher; Fiscal Year: 2010
    ..New therapeutic strategies that could benefit more broadly include exon skipping strategies that are presently being applied to the spectrin family protein dystrophin [Goyenvalle Science 2006]. ..