Alexander Pushkin

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint Two C-terminal variants of NBC4, a new member of the sodium bicarbonate cotransporter family: cloning, characterization, and localization
    A Pushkin
    Division of Nephrology, UCLA School of Medicine, Los Angeles, California 90095 1689, USA
    IUBMB Life 50:13-9. 2000
  2. ncbi request reprint Oligomeric structure of bAE3 protein
    A V Pushkin
    Division of Nephrology, Center for Health Sciences, UCLA School of Medicine, Los Angeles, California 90095 1689, USA
    IUBMB Life 50:397-401. 2000
  3. pmc Molecular mechanism of kNBC1-carbonic anhydrase II interaction in proximal tubule cells
    Alexander Pushkin
    Division of Nephrology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
    J Physiol 559:55-65. 2004
  4. ncbi request reprint Genomic organization of the DCTN1-SLC4A5 locus encoding both NBC4 and p150(Glued)
    A Pushkin
    Division of Nephrology, UCLA School of Medicine, Los Angeles, CA 90095, USA
    Cytogenet Cell Genet 95:163-8. 2001
  5. ncbi request reprint Structural characterization, tissue distribution, and functional expression of murine aminoacylase III
    Alexander Pushkin
    Division of Neurology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095 1689, USA
    Am J Physiol Cell Physiol 286:C848-56. 2004
  6. pmc NBCe1-A Transmembrane Segment 1 Lines the Ion Translocation Pathway
    Quansheng Zhu
    Division of Nephrology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095 1689, USA
    J Biol Chem 284:8918-29. 2009
  7. pmc Topological location and structural importance of the NBCe1-A residues mutated in proximal renal tubular acidosis
    Quansheng Zhu
    Division of Nephrology, David Geffen School of Medicine, UCLA, Los Angeles, California 90095, USA
    J Biol Chem 285:13416-26. 2010
  8. pmc Oligomeric structure and minimal functional unit of the electrogenic sodium bicarbonate cotransporter NBCe1-A
    Liyo Kao
    Division of Nephrology, David Geffen School Medicine, UCLA, Los Angeles, California 90095 1689, USA
    J Biol Chem 283:26782-94. 2008
  9. pmc Structural and functional characterization of the C-terminal transmembrane region of NBCe1-A
    Quansheng Zhu
    Division of Nephrology, David Geffen School of Medicine, UCLA, Los Angeles, California 90095 1689, USA
    J Biol Chem 285:37178-87. 2010
  10. pmc Critical amino acid residues involved in the electrogenic sodium-bicarbonate cotransporter kNBC1-mediated transport
    Natalia Abuladze
    Division of Nephrology, David Geffen School of Medicine at UCLA, 10833 Le Conte Avenue, Room 7 155 Factor Building, Los Angeles, CA 90095, USA
    J Physiol 565:717-30. 2005

Research Grants

  1. Biology of Mercapturic Acid Mediated Nephrotoxicity
    Alexander Pushkin; Fiscal Year: 2007
  2. Biology of Mercapturic Acid Mediated Nephrotoxicity
    Alexander Pushkin; Fiscal Year: 2009

Collaborators

  • Ira Kurtz
  • Eitan Gross
  • Wolfgang Dekant
  • V Tsuprun
  • Talya Wolak
  • Anatol Bragin
  • Sherri M Jones
  • Piotr Ruchala
  • Janos Peti-Peterdi
  • Ivan Lopez
  • Shulei Zhao
  • G Sachs
  • K F Faull
  • Steven Nusinowitz
  • Natalia Abuladze
  • Debra Newman
  • Kirill Tsirulnikov
  • Quansheng Zhu
  • Weixin Liu
  • Rustam Azimov
  • Liyo Kao
  • Pakan Sassani
  • Sergey Ryazantsev
  • Sergei Tatishchev
  • Galyna Bondar
  • Jennifer M Hsieh
  • Myong Chul Koag
  • Nathaniel Magilnick
  • Karoline Scholz
  • Dean Bok
  • Ramanath Dukkipati
  • Pejvak Sassani
  • Meeyoung Park
  • Duilio Cascio
  • Martin Phillips
  • Ivo Atanasov
  • Ritu Vahi
  • Matthew J Schibler
  • Robert Damoiseaux
  • Christopher M Ryan
  • Huma Hasnain
  • Connie Chang
  • Nuraly K Avliyakulov
  • Jeff Abramson
  • Michael R Sawaya
  • Nicholas Orozco
  • Charles Montgomery
  • Isaac Van Sligtenhorst
  • Wenhu Huang
  • Kenneth Platt
  • Akira Ishiyama
  • David Weeks
  • Carl Friddle
  • Mary Jean Sparks
  • Robert Geske
  • Michael Woodruff
  • Gary Galbraith
  • Hector BeltrandelRio
  • Gerardo Carpenito
  • Min Goo Lee
  • Myeong Sok Lee
  • Joo Young Choi
  • Joo Young Kim
  • Philip J Thomas
  • Shigeru B H Ko
  • Alla Gomer
  • Shmuel Muallem
  • Gaia Muallem

Detail Information

Publications28

  1. ncbi request reprint Two C-terminal variants of NBC4, a new member of the sodium bicarbonate cotransporter family: cloning, characterization, and localization
    A Pushkin
    Division of Nephrology, UCLA School of Medicine, Los Angeles, California 90095 1689, USA
    IUBMB Life 50:13-9. 2000
    ..In comparison with other members of the bicarbonate transport superfamily, NBC4a and NBC4b are most similar structurally to the electrogenic sodium bicarbonate cotransporters (NBC1)...
  2. ncbi request reprint Oligomeric structure of bAE3 protein
    A V Pushkin
    Division of Nephrology, Center for Health Sciences, UCLA School of Medicine, Los Angeles, California 90095 1689, USA
    IUBMB Life 50:397-401. 2000
    ..We conclude that bAE3 in Triton X-100 solution is predominantly a mixture of dimers and tetramers with a smaller amount of monomers...
  3. pmc Molecular mechanism of kNBC1-carbonic anhydrase II interaction in proximal tubule cells
    Alexander Pushkin
    Division of Nephrology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
    J Physiol 559:55-65. 2004
    ..These data are the first direct evidence that a complex of an electrogenic sodium bicarbonate cotransporter with CAII functions as a transport metabolon...
  4. ncbi request reprint Genomic organization of the DCTN1-SLC4A5 locus encoding both NBC4 and p150(Glued)
    A Pushkin
    Division of Nephrology, UCLA School of Medicine, Los Angeles, CA 90095, USA
    Cytogenet Cell Genet 95:163-8. 2001
    ..This information should allow the study of potential genomic alterations of DCTN1-SLC4A5 in patients with diseases mapping to this genomic region...
  5. ncbi request reprint Structural characterization, tissue distribution, and functional expression of murine aminoacylase III
    Alexander Pushkin
    Division of Neurology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095 1689, USA
    Am J Physiol Cell Physiol 286:C848-56. 2004
    ..The predominant cytoplasmic localization of aminoacylase III may explain the greater sensitivity of the proximal straight tubule to the nephrotoxicity of mercapturic acids...
  6. pmc NBCe1-A Transmembrane Segment 1 Lines the Ion Translocation Pathway
    Quansheng Zhu
    Division of Nephrology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095 1689, USA
    J Biol Chem 284:8918-29. 2009
    ..Our findings are suggestive of a delta(-) helical dipole at the C-terminal end of TM1 involving Thr-442 that plays a critical role in the function of the cotransporter...
  7. pmc Topological location and structural importance of the NBCe1-A residues mutated in proximal renal tubular acidosis
    Quansheng Zhu
    Division of Nephrology, David Geffen School of Medicine, UCLA, Los Angeles, California 90095, USA
    J Biol Chem 285:13416-26. 2010
    ..Our findings demonstrate that the pRTA residues in NBCe1-A are buried in the protein complex/lipid bilayer where they perform important structural roles...
  8. pmc Oligomeric structure and minimal functional unit of the electrogenic sodium bicarbonate cotransporter NBCe1-A
    Liyo Kao
    Division of Nephrology, David Geffen School Medicine, UCLA, Los Angeles, California 90095 1689, USA
    J Biol Chem 283:26782-94. 2008
    ..Our results demonstrate that wt-NBCe1-A is predominantly a homodimer, dependent on S-S bond formation that is composed of functionally active monomers...
  9. pmc Structural and functional characterization of the C-terminal transmembrane region of NBCe1-A
    Quansheng Zhu
    Division of Nephrology, David Geffen School of Medicine, UCLA, Los Angeles, California 90095 1689, USA
    J Biol Chem 285:37178-87. 2010
    ..Our findings suggest that the C-terminal transmembrane region of NBCe1-A is tightly folded with unique structural and functional features that differ from AE1...
  10. pmc Critical amino acid residues involved in the electrogenic sodium-bicarbonate cotransporter kNBC1-mediated transport
    Natalia Abuladze
    Division of Nephrology, David Geffen School of Medicine at UCLA, 10833 Le Conte Avenue, Room 7 155 Factor Building, Los Angeles, CA 90095, USA
    J Physiol 565:717-30. 2005
    ..A model summarizing key amino acid residues involved in kNBC1-mediated ion transport is presented...
  11. pmc Transport of N-acetyl-S-(1,2-dichlorovinyl)-L-cysteine, a metabolite of trichloroethylene, by mouse multidrug resistance associated protein 2 (Mrp2)
    Kirill Tsirulnikov
    Department of Medicine Nephrology, David Geffen School of Medicine, University of California at Los Angeles, CA 90095, USA
    Toxicol Appl Pharmacol 244:218-25. 2010
    ..The results suggest that Mrp2 may be involved in the renal secretion of Ac-DCVC...
  12. pmc G418-mediated ribosomal read-through of a nonsense mutation causing autosomal recessive proximal renal tubular acidosis
    Rustam Azimov
    Division of Nephrology, David Geffen School of Medicine at UCLA, University of California Los Angeles, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA
    Am J Physiol Renal Physiol 295:F633-41. 2008
    ..Our results provide the first demonstration of a mutation in NBCe1-A that has been treated in a targeted and specific manner...
  13. ncbi request reprint Specificity of aminoacylase III-mediated deacetylation of mercapturic acids
    Debra Newman
    Division of Nephrology, David Geffen School of Medicine at UCLA, 10833 Le Conte Avenue, 7 155 Factor Building, Los Angeles, CA 90095 1689, USA
    Drug Metab Dispos 35:43-50. 2007
    ..Our results indicate that in proximal tubule cells, AAIII plays an important role in deacetylating several halogenated mercapturic acids, and this process may be involved in their cyto- and nephrotoxicity...
  14. pmc Aminoacylase 3 binds to and cleaves the N-terminus of the hepatitis C virus core protein
    Kirill Tsirulnikov
    Division of Nephrology, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, CA 90095, USA
    FEBS Lett 586:3799-804. 2012
    ..Here we demonstrate that AA3 directly binds to HCVCP (K(d) ~10 μM) that may by implicated in HCV pathogenesis. AA3 also revealed a weak endopeptidase activity towards the N-terminus of HCVCP...
  15. ncbi request reprint The COOH termini of NBC3 and the 56-kDa H+-ATPase subunit are PDZ motifs involved in their interaction
    Alexander Pushkin
    Division of Nephrology, David Geffen School of Medicine, University of California, Los Angeles 90095 1689, USA
    Am J Physiol Cell Physiol 284:C667-73. 2003
    ..NHERF-1 is involved in the interaction of NBC3 and the vacuolar proton pump...
  16. ncbi request reprint Structural characterization of dimeric murine aminoacylase III
    Sergey Ryazantsev
    Department of Biological Chemistry, David Geffen School of Medicine, University of California at Los Angeles, Room 33 080 CHS, 10833 Le Conte Avenue, CA 90095 1689, USA
    FEBS Lett 581:1898-902. 2007
    ..A 16A resolution model of the AAIII dimer was created. It has an unusual, cage-like, structure. A realistic AAIII tetramer model was built from two dimers...
  17. ncbi request reprint Identification of membrane topography of the electrogenic sodium bicarbonate cotransporter pNBC1 by in vitro transcription/translation
    Sergei Tatishchev
    Division of Nephrology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    Biochemistry 42:755-65. 2003
    ..This is the first characterization of the membrane topography of a sodium bicarbonate cotransporter...
  18. pmc Structures of aminoacylase 3 in complex with acetylated substrates
    Jennifer M Hsieh
    Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 107:17962-7. 2010
    ..These structures, in conjunction with biochemical data, indicated that AA3 mediates substrate specificity through van der Waals interactions providing a dynamic interaction interface, which facilitates a diverse range of substrates...
  19. pmc Mouse aminoacylase 3: a metalloenzyme activated by cobalt and nickel
    Kirill Tsirulnikov
    Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, CA 90095, USA
    Biochim Biophys Acta 1794:1049-57. 2009
    ..The results indicate that AA3 is a metalloenzyme significantly activated by Co(2+) and Ni(2+)...
  20. ncbi request reprint Expression and localization of rat NBC4c in liver and renal uroepithelium
    Natalia Abuladze
    Division of Nephrology, University of California Los Angeles, 10833 Le Conte Avenue, Rm 7 155 Factor Bldg, Los Angeles, CA 90095 1689, USA
    Am J Physiol Cell Physiol 287:C781-9. 2004
    ....
  21. doi request reprint Inhibition of aminoacylase 3 protects rat brain cortex neuronal cells from the toxicity of 4-hydroxy-2-nonenal mercapturate and 4-hydroxy-2-nonenal
    Kirill Tsirulnikov
    Department of Medicine, University of California at Los Angeles, CA 90095 1689, USA
    Toxicol Appl Pharmacol 263:303-14. 2012
    ..The results suggest that the AA3 mediated deacetylation of 4HNE-mercapturate may be involved in the neurotoxicity of 4HNE...
  22. pmc Slc4a11 gene disruption in mice: cellular targets of sensorineuronal abnormalities
    Ivan A Lopez
    Division of Head and Neck, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    J Biol Chem 284:26882-96. 2009
    ....
  23. ncbi request reprint Functional characterization of NBC4: a new electrogenic sodium-bicarbonate cotransporter
    Pejvak Sassani
    Division of Nephrology, Center for Health Sciences, UCLA School of Medicine, Los Angeles, California 90095 1698, USA
    Am J Physiol Cell Physiol 282:C408-16. 2002
    ....
  24. ncbi request reprint SLC4 base (HCO3 -, CO3 2-) transporters: classification, function, structure, genetic diseases, and knockout models
    Alexander Pushkin
    Division of Nephrology, David Geffen School of Medicine at UCLA, University of California Los Angeles, 10833 Le Conte Avenue, Rm 7 155 Factor Bldg, Los Angeles, CA 90095, USA
    Am J Physiol Renal Physiol 290:F580-99. 2006
    ..This review focuses on recent advances in our understanding of the molecular organization and functional properties of SLC4 transporters and their role in disease...
  25. doi request reprint Measurement of aminoacylases
    Alexander Pushkin
    UCLA David Geffen School of Medicine, Los Angeles, California, USA
    Curr Protoc Toxicol . 2008
    ..The unit also provides methods for isolation of the aminoacylase III from tissues and bacterial or mammalian cells and subsequent purification and analysis...
  26. ncbi request reprint Blindness and auditory impairment caused by loss of the sodium bicarbonate cotransporter NBC3
    Dean Bok
    Department of Neurobiology, Jules Stein Eye Institute, Los Angeles, California, 90095, USA
    Nat Genet 34:313-9. 2003
    ..Our results indicate that in certain sensory organs, in which the requirement to transduce specific environmental signals with speed, sensitivity and reliability is paramount, the choice of the H+ disposal mechanism used is limited...
  27. pmc Regulation of the sodium bicarbonate cotransporter kNBC1 function: role of Asp(986), Asp(988) and kNBC1-carbonic anhydrase II binding
    Eitan Gross
    Department of Urology, Case Western Reserve University, Cleveland, OH, USA
    J Physiol 544:679-85. 2002
    ..Although CAII does not affect the transport stoichiometry, it may play an important role in enhancing the flux through the transporter when kNBC1-Ser(982) is unphosphorylated...
  28. ncbi request reprint The cystic fibrosis transmembrane conductance regulator interacts with and regulates the activity of the HCO3- salvage transporter human Na+-HCO3- cotransport isoform 3
    Meeyoung Park
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 277:50503-9. 2002
    ....

Research Grants5

  1. Biology of Mercapturic Acid Mediated Nephrotoxicity
    Alexander Pushkin; Fiscal Year: 2007
    ..Successful completion of this project will enhance our understanding of the mechanism of mercapturic acid-mediated nephrotoxicity. ..
  2. Biology of Mercapturic Acid Mediated Nephrotoxicity
    Alexander Pushkin; Fiscal Year: 2009
    ..Successful completion of this project will enhance our understanding of the mechanism of mercapturic acid-mediated nephrotoxicity. ..