Research Topics
Genomes and Genes | Alexander PushkinSummaryAffiliation: University of California Country: USA Publications
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Publications
Two C-terminal variants of NBC4, a new member of the sodium bicarbonate cotransporter family: cloning, characterization, and localizationA Pushkin
Division of Nephrology, UCLA School of Medicine, Los Angeles, California 90095 1689, USA
IUBMB Life 50:13-9. 2000..In comparison with other members of the bicarbonate transport superfamily, NBC4a and NBC4b are most similar structurally to the electrogenic sodium bicarbonate cotransporters (NBC1)...
Molecular mechanism of kNBC1-carbonic anhydrase II interaction in proximal tubule cellsAlexander Pushkin
Division of Nephrology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
J Physiol 559:55-65. 2004..These data are the first direct evidence that a complex of an electrogenic sodium bicarbonate cotransporter with CAII functions as a transport metabolon...- Structural characterization, tissue distribution, and functional expression of murine aminoacylase IIIAlexander Pushkin
Division of Neurology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095 1689, USA
Am J Physiol Cell Physiol 286:C848-56. 2004..The predominant cytoplasmic localization of aminoacylase III may explain the greater sensitivity of the proximal straight tubule to the nephrotoxicity of mercapturic acids... - Genomic organization of the DCTN1-SLC4A5 locus encoding both NBC4 and p150(Glued)A Pushkin
Division of Nephrology, UCLA School of Medicine, Los Angeles, CA 90095, USA
Cytogenet Cell Genet 95:163-8. 2001..This information should allow the study of potential genomic alterations of DCTN1-SLC4A5 in patients with diseases mapping to this genomic region... - Oligomeric structure of bAE3 proteinA V Pushkin
Division of Nephrology, Center for Health Sciences, UCLA School of Medicine, Los Angeles, California 90095 1689, USA
IUBMB Life 50:397-401. 2000..We conclude that bAE3 in Triton X-100 solution is predominantly a mixture of dimers and tetramers with a smaller amount of monomers... - NBCe1-A Transmembrane Segment 1 Lines the Ion Translocation PathwayQuansheng Zhu
Division of Nephrology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095 1689, USA
J Biol Chem 284:8918-29. 2009..Our findings are suggestive of a delta(-) helical dipole at the C-terminal end of TM1 involving Thr-442 that plays a critical role in the function of the cotransporter... - Topological location and structural importance of the NBCe1-A residues mutated in proximal renal tubular acidosisQuansheng Zhu
Division of Nephrology, David Geffen School of Medicine, UCLA, Los Angeles, California 90095, USA
J Biol Chem 285:13416-26. 2010..Our findings demonstrate that the pRTA residues in NBCe1-A are buried in the protein complex/lipid bilayer where they perform important structural roles... - Oligomeric structure and minimal functional unit of the electrogenic sodium bicarbonate cotransporter NBCe1-ALiyo Kao
Division of Nephrology, David Geffen School Medicine, UCLA, Los Angeles, California 90095 1689, USA
J Biol Chem 283:26782-94. 2008..Our results demonstrate that wt-NBCe1-A is predominantly a homodimer, dependent on S-S bond formation that is composed of functionally active monomers... - Structural and functional characterization of the C-terminal transmembrane region of NBCe1-AQuansheng Zhu
Division of Nephrology, David Geffen School of Medicine, UCLA, Los Angeles, California 90095 1689, USA
J Biol Chem 285:37178-87. 2010..Our findings suggest that the C-terminal transmembrane region of NBCe1-A is tightly folded with unique structural and functional features that differ from AE1... - Critical amino acid residues involved in the electrogenic sodium-bicarbonate cotransporter kNBC1-mediated transportNatalia Abuladze
Division of Nephrology, David Geffen School of Medicine at UCLA, 10833 Le Conte Avenue, Room 7 155 Factor Building, Los Angeles, CA 90095, USA
J Physiol 565:717-30. 2005..A model summarizing key amino acid residues involved in kNBC1-mediated ion transport is presented... - G418-mediated ribosomal read-through of a nonsense mutation causing autosomal recessive proximal renal tubular acidosisRustam Azimov
Division of Nephrology, David Geffen School of Medicine at UCLA, University of California Los Angeles, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA
Am J Physiol Renal Physiol 295:F633-41. 2008..Our results provide the first demonstration of a mutation in NBCe1-A that has been treated in a targeted and specific manner... - Transport of N-acetyl-S-(1,2-dichlorovinyl)-L-cysteine, a metabolite of trichloroethylene, by mouse multidrug resistance associated protein 2 (Mrp2)Kirill Tsirulnikov
Department of Medicine Nephrology, David Geffen School of Medicine, University of California at Los Angeles, CA 90095, USA
Toxicol Appl Pharmacol 244:218-25. 2010..The results suggest that Mrp2 may be involved in the renal secretion of Ac-DCVC... - Specificity of aminoacylase III-mediated deacetylation of mercapturic acidsDebra Newman
Division of Nephrology, David Geffen School of Medicine at UCLA, 10833 Le Conte Avenue, 7 155 Factor Building, Los Angeles, CA 90095 1689, USA
Drug Metab Dispos 35:43-50. 2007..Our results indicate that in proximal tubule cells, AAIII plays an important role in deacetylating several halogenated mercapturic acids, and this process may be involved in their cyto- and nephrotoxicity... - The COOH termini of NBC3 and the 56-kDa H+-ATPase subunit are PDZ motifs involved in their interactionAlexander Pushkin
Division of Nephrology, David Geffen School of Medicine, University of California, Los Angeles 90095 1689, USA
Am J Physiol Cell Physiol 284:C667-73. 2003..NHERF-1 is involved in the interaction of NBC3 and the vacuolar proton pump... - Mouse aminoacylase 3: a metalloenzyme activated by cobalt and nickelKirill Tsirulnikov
Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, CA 90095, USA
Biochim Biophys Acta 1794:1049-57. 2009..The results indicate that AA3 is a metalloenzyme significantly activated by Co(2+) and Ni(2+)... - Structural characterization of dimeric murine aminoacylase IIISergey Ryazantsev
Department of Biological Chemistry, David Geffen School of Medicine, University of California at Los Angeles, Room 33 080 CHS, 10833 Le Conte Avenue, CA 90095 1689, USA
FEBS Lett 581:1898-902. 2007..A 16A resolution model of the AAIII dimer was created. It has an unusual, cage-like, structure. A realistic AAIII tetramer model was built from two dimers... - Identification of membrane topography of the electrogenic sodium bicarbonate cotransporter pNBC1 by in vitro transcription/translationSergei Tatishchev
Division of Nephrology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
Biochemistry 42:755-65. 2003..This is the first characterization of the membrane topography of a sodium bicarbonate cotransporter... - Structures of aminoacylase 3 in complex with acetylated substratesJennifer M Hsieh
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA
Proc Natl Acad Sci U S A 107:17962-7. 2010..These structures, in conjunction with biochemical data, indicated that AA3 mediates substrate specificity through van der Waals interactions providing a dynamic interaction interface, which facilitates a diverse range of substrates... - Expression and localization of rat NBC4c in liver and renal uroepitheliumNatalia Abuladze
Division of Nephrology, University of California Los Angeles, 10833 Le Conte Avenue, Rm 7 155 Factor Bldg, Los Angeles, CA 90095 1689, USA
Am J Physiol Cell Physiol 287:C781-9. 2004.... - Inhibition of aminoacylase 3 protects rat brain cortex neuronal cells from the toxicity of 4-hydroxy-2-nonenal mercapturate and 4-hydroxy-2-nonenalKirill Tsirulnikov
Department of Medicine, University of California at Los Angeles, CA 90095 1689, USA
Toxicol Appl Pharmacol 263:303-14. 2012..The results suggest that the AA3 mediated deacetylation of 4HNE-mercapturate may be involved in the neurotoxicity of 4HNE... - Functional characterization of NBC4: a new electrogenic sodium-bicarbonate cotransporterPejvak Sassani
Division of Nephrology, Center for Health Sciences, UCLA School of Medicine, Los Angeles, California 90095 1698, USA
Am J Physiol Cell Physiol 282:C408-16. 2002.... - Slc4a11 gene disruption in mice: cellular targets of sensorineuronal abnormalitiesIvan A Lopez
Division of Head and Neck, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
J Biol Chem 284:26882-96. 2009.... - SLC4 base (HCO3 -, CO3 2-) transporters: classification, function, structure, genetic diseases, and knockout modelsAlexander Pushkin
Division of Nephrology, David Geffen School of Medicine at UCLA, University of California-Los Angeles, 10833 Le Conte Avenue, Rm. 7-155 Factor Bldg, Los Angeles, CA 90095, USA
Am J Physiol Renal Physiol 290:F580-99. 2006..This review focuses on recent advances in our understanding of the molecular organization and functional properties of SLC4 transporters and their role in disease... - Aminoacylase 3 binds to and cleaves the N-terminus of the hepatitis C virus core proteinKirill Tsirulnikov
Division of Nephrology, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, CA 90095, USA Electronic address
FEBS Lett 586:3799-804. 2012..STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: AA3cleavesHCVCP by protease assay (View interaction). AA3cleavesAA3 by protease assay (View interaction). AA3binds to HCVCP by surface plasmon resonance (View Interaction:1,2,3,4,5)... - Blindness and auditory impairment caused by loss of the sodium bicarbonate cotransporter NBC3Dean Bok
Department of Neurobiology, Jules Stein Eye Institute, Los Angeles, California, 90095, USA
Nat Genet 34:313-9. 2003..Our results indicate that in certain sensory organs, in which the requirement to transduce specific environmental signals with speed, sensitivity and reliability is paramount, the choice of the H+ disposal mechanism used is limited... - Regulation of the sodium bicarbonate cotransporter kNBC1 function: role of Asp(986), Asp(988) and kNBC1-carbonic anhydrase II bindingEitan Gross
Department of Urology, Case Western Reserve University, Cleveland, OH, USA
J Physiol 544:679-85. 2002..Although CAII does not affect the transport stoichiometry, it may play an important role in enhancing the flux through the transporter when kNBC1-Ser(982) is unphosphorylated... - The cystic fibrosis transmembrane conductance regulator interacts with and regulates the activity of the HCO3- salvage transporter human Na+-HCO3- cotransport isoform 3Meeyoung Park
Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
J Biol Chem 277:50503-9. 2002....
Research Grants
- Biology of Mercapturic Acid Mediated NephrotoxicityAlexander Pushkin; Fiscal Year: 2007..Successful completion of this project will enhance our understanding of the mechanism of mercapturic acid-mediated nephrotoxicity. ..
- Biology of Mercapturic Acid Mediated NephrotoxicityAlexander Pushkin; Fiscal Year: 2009..Successful completion of this project will enhance our understanding of the mechanism of mercapturic acid-mediated nephrotoxicity. ..