Paolo P Provenzano

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. doi request reprint Multiphoton microscopy and fluorescence lifetime imaging microscopy (FLIM) to monitor metastasis and the tumor microenvironment
    Paolo P Provenzano
    Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA
    Clin Exp Metastasis 26:357-70. 2009
  2. pmc Systemic administration of IGF-I enhances healing in collagenous extracellular matrices: evaluation of loaded and unloaded ligaments
    Paolo P Provenzano
    Dept of Biomedical Engineering, University of Wisconsin, Madison, WI, USA
    BMC Physiol 7:2. 2007
  3. pmc Collagen density promotes mammary tumor initiation and progression
    Paolo P Provenzano
    Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA
    BMC Med 6:11. 2008
  4. ncbi request reprint Collagen fibril morphology and organization: implications for force transmission in ligament and tendon
    Paolo P Provenzano
    Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USA
    Matrix Biol 25:71-84. 2006
  5. ncbi request reprint Intrinsic fibroblast-mediated remodeling of damaged collagenous matrices in vivo
    Paolo P Provenzano
    Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA
    Matrix Biol 23:543-55. 2005
  6. pmc Contact guidance mediated three-dimensional cell migration is regulated by Rho/ROCK-dependent matrix reorganization
    Paolo P Provenzano
    Department of Pharmacology, Laboratory of Molecular Biology, University of Wisconsin Paul P Carbone Comprehensive Cancer Center, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biophys J 95:5374-84. 2008
  7. doi request reprint Nonlinear optical microscopy and computational analysis of intrinsic signatures in breast cancer
    Curtis T Rueden
    Laboratory for Optical and Computational Instrumentation, University of Wisconsin, Madison, WI 53706 USA
    Conf Proc IEEE Eng Med Biol Soc 2009:4077-80. 2009
  8. doi request reprint Nonlinear optical imaging and spectral-lifetime computational analysis of endogenous and exogenous fluorophores in breast cancer
    Paolo P Provenzano
    University of Wisconsin, Departments of Pharmacology and Biomedical Engineering and Laboratory for Optical and Computational Instrumentation and Paul P Carbone Comprehensive Cancer Center, Madison, Wisconsin 53706, USA
    J Biomed Opt 13:031220. 2008
  9. ncbi request reprint Adjuvant neuropeptides can improve neuropathic ligament healing in a rat model
    Kelley W Grorud
    Orthopedic Research Laboratories, Department of Orthopedics and Rehabilitation, University of Wisconsin, 600 Highland Avenue, Madison, WI 53792, USA
    J Orthop Res 25:703-12. 2007
  10. pmc Mammary epithelial-specific disruption of focal adhesion kinase retards tumor formation and metastasis in a transgenic mouse model of human breast cancer
    Paolo P Provenzano
    Department of Pharmacology, Laboratory of Molecular Biology, Madison, WI 53706, USA ppproven wisc edu
    Am J Pathol 173:1551-65. 2008

Collaborators

Detail Information

Publications27

  1. doi request reprint Multiphoton microscopy and fluorescence lifetime imaging microscopy (FLIM) to monitor metastasis and the tumor microenvironment
    Paolo P Provenzano
    Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA
    Clin Exp Metastasis 26:357-70. 2009
    ..Herein, we review recent developments of multiphoton FLIM and SLIM to study 2D and 3D cell migration, invasion into the tumor microenvironment, and metastasis...
  2. pmc Systemic administration of IGF-I enhances healing in collagenous extracellular matrices: evaluation of loaded and unloaded ligaments
    Paolo P Provenzano
    Dept of Biomedical Engineering, University of Wisconsin, Madison, WI, USA
    BMC Physiol 7:2. 2007
    ..After 3 weeks, mechanical properties, cell and matrix morphology, and biochemical composition were examined in control and healing ligaments...
  3. pmc Collagen density promotes mammary tumor initiation and progression
    Paolo P Provenzano
    Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA
    BMC Med 6:11. 2008
    ..Importantly, regions of high breast density are associated with increased stromal collagen. Thus, the influence of the extracellular matrix on breast carcinoma development and the underlying molecular mechanisms are not understood...
  4. ncbi request reprint Collagen fibril morphology and organization: implications for force transmission in ligament and tendon
    Paolo P Provenzano
    Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USA
    Matrix Biol 25:71-84. 2006
    ..Based upon this information and published data, we conclude that force within these tissues is directly transferred through collagen fibrils and not through an interfibrillar coupling, such as a proteoglycan bridge...
  5. ncbi request reprint Intrinsic fibroblast-mediated remodeling of damaged collagenous matrices in vivo
    Paolo P Provenzano
    Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA
    Matrix Biol 23:543-55. 2005
    ..As such, this system provides unique insight into acute tissue damage and offers a potentially powerful model to examine fibroblast behavior...
  6. pmc Contact guidance mediated three-dimensional cell migration is regulated by Rho/ROCK-dependent matrix reorganization
    Paolo P Provenzano
    Department of Pharmacology, Laboratory of Molecular Biology, University of Wisconsin Paul P Carbone Comprehensive Cancer Center, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biophys J 95:5374-84. 2008
    ....
  7. doi request reprint Nonlinear optical microscopy and computational analysis of intrinsic signatures in breast cancer
    Curtis T Rueden
    Laboratory for Optical and Computational Instrumentation, University of Wisconsin, Madison, WI 53706 USA
    Conf Proc IEEE Eng Med Biol Soc 2009:4077-80. 2009
    ..In particular we present the latest development in our SLIM Plotter application, an open source visualization program for interactive visualization and inspection of combined spectral lifetime (SLIM) data...
  8. doi request reprint Nonlinear optical imaging and spectral-lifetime computational analysis of endogenous and exogenous fluorophores in breast cancer
    Paolo P Provenzano
    University of Wisconsin, Departments of Pharmacology and Biomedical Engineering and Laboratory for Optical and Computational Instrumentation and Paul P Carbone Comprehensive Cancer Center, Madison, Wisconsin 53706, USA
    J Biomed Opt 13:031220. 2008
    ..Using SLIM, we present a method for imaging and differentiating signals from multiple fluorophores that can have overlapping spectra via SLIM Plotter-a computational tool for visualizing and analyzing large spectral-lifetime data sets...
  9. ncbi request reprint Adjuvant neuropeptides can improve neuropathic ligament healing in a rat model
    Kelley W Grorud
    Orthopedic Research Laboratories, Department of Orthopedics and Rehabilitation, University of Wisconsin, 600 Highland Avenue, Madison, WI 53792, USA
    J Orthop Res 25:703-12. 2007
    ..In addition, neuropeptide Y stimulated MCL healing in this model. These findings suggest a new paradigm to improve neuropathic soft connective tissue healing...
  10. pmc Mammary epithelial-specific disruption of focal adhesion kinase retards tumor formation and metastasis in a transgenic mouse model of human breast cancer
    Paolo P Provenzano
    Department of Pharmacology, Laboratory of Molecular Biology, Madison, WI 53706, USA ppproven wisc edu
    Am J Pathol 173:1551-65. 2008
    ..Therefore, FAK acts as a primary node in the activated signaling network in transformed motile cells and is a prime candidate for novel therapeutic interventions to treat aggressive human breast cancers...
  11. ncbi request reprint Application of a probabilistic microstructural model to determine reference length and toe-to-linear region transition in fibrous connective tissue
    Christof Hurschler
    Department of Orthopedics and Rehabilitation, University of Wisconsin, Madison, WI 53792 7375, USA
    J Biomech Eng 125:415-22. 2003
    ....
  12. pmc Collagen reorganization at the tumor-stromal interface facilitates local invasion
    Paolo P Provenzano
    Department of Pharmacology, University of Wisconsin, Madison, WI, USA
    BMC Med 4:38. 2006
    ....
  13. doi request reprint Nonlinear optical imaging of cellular processes in breast cancer
    Paolo P Provenzano
    Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA
    Microsc Microanal 14:532-48. 2008
    ..Furthermore, the ability to record and monitor the intrinsic fluorescence and SHG signals provides a unique tool for researchers to understand key events in cancer progression in its natural context...
  14. doi request reprint Fluorescence lifetime imaging of endogenous fluorophores in histopathology sections reveals differences between normal and tumor epithelium in carcinoma in situ of the breast
    Matthew W Conklin
    Department of Pharmacology, University of Wisconsin, 1525 Linden Dr, Madison, WI 53706, USA
    Cell Biochem Biophys 53:145-57. 2009
    ..Most forms of cancer have altered metabolism and redox ratios; here we present a method that has potential for early detection of these changes, which are preserved in fixed tissue samples such as classic histopathology slides...
  15. pmc Shining new light on 3D cell motility and the metastatic process
    Paolo P Provenzano
    Laboratory for Optical and Computational Instrumentation, and Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA
    Trends Cell Biol 19:638-48. 2009
    ....
  16. pmc Aligned collagen is a prognostic signature for survival in human breast carcinoma
    Matthew W Conklin
    Department of Pharmacology, and the Laboratory for Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Am J Pathol 178:1221-32. 2011
    ....
  17. ncbi request reprint Nonlinear viscoelasticity in rabbit medial collateral ligament
    Rittu V Hingorani
    Orthopedic Research Laboratories, Department of Orthopedics and Rehabilitation, University of Wisconsin, Madison, Wisconsin 53792, USA
    Ann Biomed Eng 32:306-12. 2004
    ..As such, these nonlinearities should be considered when quantifying ligament viscoelastic behavior with a rabbit model...
  18. ncbi request reprint Required test duration for group comparisons in ligament viscoelasticity: a statistical approach
    Eugene Manley
    Department of Orthopedics and Rehabilitation, University of Wisconsin, Madison, WI 53792, USA
    Biorheology 40:441-50. 2003
    ..Hence, a better way to compare viscoelastic behavior between study groups is to test more specimens for shorter durations. This reduces experimental time per sample and therefore increases efficiency...
  19. ncbi request reprint Scanning electron microscopic characterization of healing and normal rat ligament microstructure under slack and loaded conditions
    Christof Hurschler
    Department of Orthopedics and Rehabilitation, University of Wisconsin Madison, Madison, Wisconsin 53792 3228, USA
    Connect Tissue Res 44:59-68. 2003
    ..The data suggest that straightening of crimped fibers is a functionally relevant phenomenon, not only in normal but also in healing ligaments...
  20. pmc The role of focal adhesion kinase in tumor initiation and progression
    Paolo P Provenzano
    Laboratory of Molecular Biology and Departments of Pharmacology and Biomedical Engineering, University of Wisconsin, Madison, WI, USA
    Cell Adh Migr 3:347-50. 2009
    ..Hence, FAK, as well as the FAK-regulating/regulated signaling network, are viable candidates for cancer metastasis therapies...
  21. ncbi request reprint Subfailure damage in ligament: a structural and cellular evaluation
    Paolo P Provenzano
    Department of Biomedical Engineering and Department of Orthopedic Surgery, School of Veterinary Medicine, University of Wisconsin, Madison 53706, USA
    J Appl Physiol 92:362-71. 2002
    ..Cellular damage was induced at ligament strains significantly below the structural damage threshold. This cellular damage is likely to be part of the natural healing process in mildly sprained ligaments...
  22. ncbi request reprint Collagen in connective tissue: from tendon to bone
    Ray Vanderby
    Orthopedic Research Laboratories, Departments of Orthopedic Surgery and Biomedical Engineering, 600 Highland Avenue, 53792, Madison, WI, USA
    J Biomech 36:1523-7. 2003
  23. ncbi request reprint Blockade of the sympathetic nervous system degrades ligament in a rat MCL model
    Kelley W Dwyer
    Department of Orthopedics and Rehabilitation, University of Wisconsin, Madison, Wisconsin 53792, USA
    J Appl Physiol 96:711-8. 2004
    ..Histology also identified increases in TRAP and cathepsin K, but not MMP-13, in guanethidine-treated tissues. Results support our hypothesis that blockade of the sympathetic nervous system substantially degrades ligament...
  24. ncbi request reprint Investigating integrin regulation and signaling events in three-dimensional systems
    Patricia J Keely
    Department of Pharmacology, University of Wisconsin Medical School, University of Wisconsin Madison, Madison, Wisconsin, USA
    Methods Enzymol 426:27-45. 2007
    ..The purpose of this chapter is to provide a few overall strategies for working within 3D culture systems, focusing on biochemical and molecular imaging approaches...
  25. ncbi request reprint A boundary layer model for wall shear stress in arterial stenosis
    Paolo P Provenzano
    Department of Biomedical Engineering, University of Wisconsin Madison, WI, USA
    Biorheology 39:743-54. 2002
    ..The boundary layer model proposed can be easily implemented by clinical researchers to provide in vivo estimates of wall shear stress through arterial stenoses...
  26. ncbi request reprint Hindlimb unloading alters ligament healing
    Paolo P Provenzano
    Orthopedic Research Laboratories, Department of Orthopedics and Rehabilitation, University of Wisconsin, Madison 53792 3228, USA
    J Appl Physiol 94:314-24. 2003
    ..In addition, this study provides compelling morphological evidence explaining the altered structure-function relationship in load-deprived healing connective tissue...
  27. ncbi request reprint Healing of subfailure ligament injury: comparison between immature and mature ligaments in a rat model
    Paolo P Provenzano
    Department of Orthopaedics and Rehabilitation, University of Wisconsin Madison, 53792 3228, USA
    J Orthop Res 20:975-83. 2002
    ..Hence, this study, with a new injury model, showed differences in ligament healing associated with maturity and quantified the clinically observed persistance of ligament laxity...