Research Topics
| Paolo P ProvenzanoSummaryAffiliation: University of Wisconsin Country: USA Publications
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Detail Information
Publications
Multiphoton microscopy and fluorescence lifetime imaging microscopy (FLIM) to monitor metastasis and the tumor microenvironmentPaolo P Provenzano
Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA
Clin Exp Metastasis 26:357-70. 2009..Herein, we review recent developments of multiphoton FLIM and SLIM to study 2D and 3D cell migration, invasion into the tumor microenvironment, and metastasis...- Systemic administration of IGF-I enhances healing in collagenous extracellular matrices: evaluation of loaded and unloaded ligamentsPaolo P Provenzano
Dept of Biomedical Engineering, University of Wisconsin, Madison, WI, USA
BMC Physiol 7:2. 2007..After 3 weeks, mechanical properties, cell and matrix morphology, and biochemical composition were examined in control and healing ligaments... - Collagen density promotes mammary tumor initiation and progressionPaolo P Provenzano
Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA
BMC Med 6:11. 2008..Importantly, regions of high breast density are associated with increased stromal collagen. Thus, the influence of the extracellular matrix on breast carcinoma development and the underlying molecular mechanisms are not understood... - Collagen fibril morphology and organization: implications for force transmission in ligament and tendonPaolo P Provenzano
Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USA
Matrix Biol 25:71-84. 2006..Based upon this information and published data, we conclude that force within these tissues is directly transferred through collagen fibrils and not through an interfibrillar coupling, such as a proteoglycan bridge... - Intrinsic fibroblast-mediated remodeling of damaged collagenous matrices in vivoPaolo P Provenzano
Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA
Matrix Biol 23:543-55. 2005..As such, this system provides unique insight into acute tissue damage and offers a potentially powerful model to examine fibroblast behavior... - Contact guidance mediated three-dimensional cell migration is regulated by Rho/ROCK-dependent matrix reorganizationPaolo P Provenzano
Department of Pharmacology, Laboratory of Molecular Biology, University of Wisconsin Paul P Carbone Comprehensive Cancer Center, University of Wisconsin, Madison, Wisconsin 53706, USA
Biophys J 95:5374-84. 2008.... - Nonlinear optical microscopy and computational analysis of intrinsic signatures in breast cancerCurtis T Rueden
Laboratory for Optical and Computational Instrumentation, University of Wisconsin, Madison, WI 53706 USA
Conf Proc IEEE Eng Med Biol Soc 2009:4077-80. 2009..In particular we present the latest development in our SLIM Plotter application, an open source visualization program for interactive visualization and inspection of combined spectral lifetime (SLIM) data... - Nonlinear optical imaging and spectral-lifetime computational analysis of endogenous and exogenous fluorophores in breast cancerPaolo P Provenzano
University of Wisconsin, Departments of Pharmacology and Biomedical Engineering and Laboratory for Optical and Computational Instrumentation and Paul P Carbone Comprehensive Cancer Center, Madison, Wisconsin 53706, USA
J Biomed Opt 13:031220. 2008..Using SLIM, we present a method for imaging and differentiating signals from multiple fluorophores that can have overlapping spectra via SLIM Plotter-a computational tool for visualizing and analyzing large spectral-lifetime data sets... - Adjuvant neuropeptides can improve neuropathic ligament healing in a rat modelKelley W Grorud
Orthopedic Research Laboratories, Department of Orthopedics and Rehabilitation, University of Wisconsin, 600 Highland Avenue, Madison, WI 53792, USA
J Orthop Res 25:703-12. 2007..In addition, neuropeptide Y stimulated MCL healing in this model. These findings suggest a new paradigm to improve neuropathic soft connective tissue healing... - Mammary epithelial-specific disruption of focal adhesion kinase retards tumor formation and metastasis in a transgenic mouse model of human breast cancerPaolo P Provenzano
Department of Pharmacology, Laboratory of Molecular Biology, Madison, WI 53706, USA ppproven wisc edu
Am J Pathol 173:1551-65. 2008..Therefore, FAK acts as a primary node in the activated signaling network in transformed motile cells and is a prime candidate for novel therapeutic interventions to treat aggressive human breast cancers... - Application of a probabilistic microstructural model to determine reference length and toe-to-linear region transition in fibrous connective tissueChristof Hurschler
Department of Orthopedics and Rehabilitation, University of Wisconsin, Madison, WI 53792-7375, USA
J Biomech Eng 125:415-22. 2003.... - Collagen reorganization at the tumor-stromal interface facilitates local invasionPaolo P Provenzano
Department of Pharmacology, University of Wisconsin, Madison, WI, USA
BMC Med 4:38. 2006.... - Nonlinear optical imaging of cellular processes in breast cancerPaolo P Provenzano
Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA
Microsc Microanal 14:532-48. 2008..Furthermore, the ability to record and monitor the intrinsic fluorescence and SHG signals provides a unique tool for researchers to understand key events in cancer progression in its natural context... - Fluorescence lifetime imaging of endogenous fluorophores in histopathology sections reveals differences between normal and tumor epithelium in carcinoma in situ of the breastMatthew W Conklin
Department of Pharmacology, University of Wisconsin, 1525 Linden Dr, Madison, WI 53706, USA
Cell Biochem Biophys 53:145-57. 2009..Most forms of cancer have altered metabolism and redox ratios; here we present a method that has potential for early detection of these changes, which are preserved in fixed tissue samples such as classic histopathology slides... - Shining new light on 3D cell motility and the metastatic processPaolo P Provenzano
Laboratory for Optical and Computational Instrumentation, and Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA
Trends Cell Biol 19:638-48. 2009.... - Aligned collagen is a prognostic signature for survival in human breast carcinomaMatthew W Conklin
Department of Pharmacology, and the Laboratory for Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
Am J Pathol 178:1221-32. 2011.... - Nonlinear viscoelasticity in rabbit medial collateral ligamentRittu V Hingorani
Orthopedic Research Laboratories, Department of Orthopedics and Rehabilitation, University of Wisconsin, Madison, Wisconsin 53792, USA
Ann Biomed Eng 32:306-12. 2004..As such, these nonlinearities should be considered when quantifying ligament viscoelastic behavior with a rabbit model... - Required test duration for group comparisons in ligament viscoelasticity: a statistical approachEugene Manley
Department of Orthopedics and Rehabilitation, University of Wisconsin, Madison, WI 53792, USA
Biorheology 40:441-50. 2003..Hence, a better way to compare viscoelastic behavior between study groups is to test more specimens for shorter durations. This reduces experimental time per sample and therefore increases efficiency... - Scanning electron microscopic characterization of healing and normal rat ligament microstructure under slack and loaded conditionsChristof Hurschler
Department of Orthopedics and Rehabilitation, University of Wisconsin--Madison, Madison, Wisconsin 53792-3228, USA
Connect Tissue Res 44:59-68. 2003..The data suggest that straightening of crimped fibers is a functionally relevant phenomenon, not only in normal but also in healing ligaments... - The role of focal adhesion kinase in tumor initiation and progressionPaolo P Provenzano
Laboratory of Molecular Biology and Departments of Pharmacology and Biomedical Engineering, University of Wisconsin, Madison, WI, USA
Cell Adh Migr 3:347-50. 2009..Hence, FAK, as well as the FAK-regulating/regulated signaling network, are viable candidates for cancer metastasis therapies... - Subfailure damage in ligament: a structural and cellular evaluationPaolo P Provenzano
Department of Biomedical Engineering and Department of Orthopedic Surgery, School of Veterinary Medicine, University of Wisconsin, Madison 53706, USA
J Appl Physiol 92:362-71. 2002..Cellular damage was induced at ligament strains significantly below the structural damage threshold. This cellular damage is likely to be part of the natural healing process in mildly sprained ligaments... - Collagen in connective tissue: from tendon to boneRay Vanderby
Orthopedic Research Laboratories, Departments of Orthopedic Surgery and Biomedical Engineering, 600 Highland Avenue, 53792, Madison, WI, USA
J Biomech 36:1523-7. 2003 - Blockade of the sympathetic nervous system degrades ligament in a rat MCL modelKelley W Dwyer
Department of Orthopedics and Rehabilitation, University of Wisconsin, Madison, Wisconsin 53792, USA
J Appl Physiol 96:711-8. 2004..Histology also identified increases in TRAP and cathepsin K, but not MMP-13, in guanethidine-treated tissues. Results support our hypothesis that blockade of the sympathetic nervous system substantially degrades ligament... - Investigating integrin regulation and signaling events in three-dimensional systemsPatricia J Keely
Department of Pharmacology, University of Wisconsin Medical School, University of Wisconsin Madison, Madison, Wisconsin, USA
Methods Enzymol 426:27-45. 2007..The purpose of this chapter is to provide a few overall strategies for working within 3D culture systems, focusing on biochemical and molecular imaging approaches... - A boundary layer model for wall shear stress in arterial stenosisPaolo P Provenzano
Department of Biomedical Engineering, University of Wisconsin Madison, WI, USA
Biorheology 39:743-54. 2002..The boundary layer model proposed can be easily implemented by clinical researchers to provide in vivo estimates of wall shear stress through arterial stenoses... - Hindlimb unloading alters ligament healingPaolo P Provenzano
U Houston, TX
J Appl Physiol 94:314-24. 2003..In addition, this study provides compelling morphological evidence explaining the altered structure-function relationship in load-deprived healing connective tissue... - Healing of subfailure ligament injury: comparison between immature and mature ligaments in a rat modelPaolo P Provenzano
Department of Orthopaedics and Rehabilitation, University of Wisconsin-Madison, 53792-3228, USA
J Orthop Res 20:975-83. 2002..Hence, this study, with a new injury model, showed differences in ligament healing associated with maturity and quantified the clinically observed persistance of ligament laxity...