P Primakoff

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint The ADAM gene family: surface proteins with adhesion and protease activity
    P Primakoff
    Department of Cell Biology and Anatomy, School of Medicine, University of California, Davis, CA 95616, USA
    Trends Genet 16:83-7. 2000
  2. ncbi request reprint Oocyte CD9 is enriched on the microvillar membrane and required for normal microvillar shape and distribution
    Kathryn E Runge
    Section of Molecular and Cellular Biology, University of California Davis, Davis, CA 95616, USA
    Dev Biol 304:317-25. 2007
  3. ncbi request reprint Penetration, adhesion, and fusion in mammalian sperm-egg interaction
    Paul Primakoff
    Department of Cell Biology, School of Medicine, University of California Davis, Davis, CA 95616, USA
    Science 296:2183-5. 2002
  4. ncbi request reprint Reversible contraceptive effect of PH-20 immunization in male guinea pigs
    P Primakoff
    Department of Physiology, University of Connecticut Health Center, Farmington 06030, USA
    Biol Reprod 56:1142-6. 1997
  5. ncbi request reprint Cell-cell membrane fusion during mammalian fertilization
    Paul Primakoff
    Department of Cell Biology and Human Anatomy, School of Medicine, University of California Davis, Davis, CA 95616, USA
    FEBS Lett 581:2174-80. 2007
  6. pmc Identification of a binding site in the disintegrin domain of fertilin required for sperm-egg fusion
    D G Myles
    Department of Physiology, University of Connecticut Health Center, Farmington 06030
    Proc Natl Acad Sci U S A 91:4195-8. 1994
  7. ncbi request reprint Fertilization defects in sperm from mice lacking fertilin beta
    C Cho
    Section of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA
    Science 281:1857-9. 1998
  8. ncbi request reprint Identification of four novel ADAMs with potential roles in spermatogenesis and fertilization
    G Z Zhu
    Department of Cell Biology and Human Anatomy, School of Medicine, University of California Davis, Davis, CA 95616, USA
    Gene 234:227-37. 1999
  9. ncbi request reprint Initial evaluation of fertilin as an immunocontraceptive antigen and molecular cloning of the cynomolgus monkey fertilin beta subunit
    C S Ramarao
    Department of Physiology, University of Connecticut Health Center, Farmington 06030 3505, USA
    Mol Reprod Dev 43:70-5. 1996
  10. pmc Molecular cloning of the human and monkey sperm surface protein PH-20
    Y Lin
    Department of Physiology, University of Connecticut Health Center, Farmington 06030
    Proc Natl Acad Sci U S A 90:10071-5. 1993

Research Grants

Collaborators

Detail Information

Publications35

  1. ncbi request reprint The ADAM gene family: surface proteins with adhesion and protease activity
    P Primakoff
    Department of Cell Biology and Anatomy, School of Medicine, University of California, Davis, CA 95616, USA
    Trends Genet 16:83-7. 2000
    ..We discuss the ADAM gene products with known functions that act in a highly diverse set of biological processes, including fertilization, neurogenesis, myogenesis, embryonic TGF-alpha release and the inflammatory response...
  2. ncbi request reprint Oocyte CD9 is enriched on the microvillar membrane and required for normal microvillar shape and distribution
    Kathryn E Runge
    Section of Molecular and Cellular Biology, University of California Davis, Davis, CA 95616, USA
    Dev Biol 304:317-25. 2007
    ..They may also have a dynamic interaction with the sperm that serves to capture the sperm cell and bring it into close contact with the oocyte plasma membrane...
  3. ncbi request reprint Penetration, adhesion, and fusion in mammalian sperm-egg interaction
    Paul Primakoff
    Department of Cell Biology, School of Medicine, University of California Davis, Davis, CA 95616, USA
    Science 296:2183-5. 2002
    ..Signaling in the sperm is induced by sperm adhesion to the zona pellucida, and signaling in the egg by gamete fusion. The gamete molecules and molecular interactions with essential roles in these events are gradually being discovered...
  4. ncbi request reprint Reversible contraceptive effect of PH-20 immunization in male guinea pigs
    P Primakoff
    Department of Physiology, University of Connecticut Health Center, Farmington 06030, USA
    Biol Reprod 56:1142-6. 1997
    ..0-4.8 x 10(3), and the precise residual titer did not correlate with fertility/infertility. The results show that immunization of males with PH-20, even at low doses, results in a reproducible, completely effective contraceptive action...
  5. ncbi request reprint Cell-cell membrane fusion during mammalian fertilization
    Paul Primakoff
    Department of Cell Biology and Human Anatomy, School of Medicine, University of California Davis, Davis, CA 95616, USA
    FEBS Lett 581:2174-80. 2007
    ..So far, no mammalian proteins have been found that also function in sperm-egg fusion of non-mammalian species or in other types of cell-cell fusion...
  6. pmc Identification of a binding site in the disintegrin domain of fertilin required for sperm-egg fusion
    D G Myles
    Department of Physiology, University of Connecticut Health Center, Farmington 06030
    Proc Natl Acad Sci U S A 91:4195-8. 1994
    ..These results show that the disintegrin domain of fertilin beta binds to the egg plasma membrane and that this binding is required for membrane fusion...
  7. ncbi request reprint Fertilization defects in sperm from mice lacking fertilin beta
    C Cho
    Section of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA
    Science 281:1857-9. 1998
    ..Fertilin could also have a direct role in sperm-zona binding or oviduct migration; alternatively, the effects on these functions could result from the absence of fertilin activity during spermatogenesis...
  8. ncbi request reprint Identification of four novel ADAMs with potential roles in spermatogenesis and fertilization
    G Z Zhu
    Department of Cell Biology and Human Anatomy, School of Medicine, University of California Davis, Davis, CA 95616, USA
    Gene 234:227-37. 1999
    ..These results suggest that each ADAM is transcribed in spermatogenic cells in a regulated pattern at a specific developmental stage...
  9. ncbi request reprint Initial evaluation of fertilin as an immunocontraceptive antigen and molecular cloning of the cynomolgus monkey fertilin beta subunit
    C S Ramarao
    Department of Physiology, University of Connecticut Health Center, Farmington 06030 3505, USA
    Mol Reprod Dev 43:70-5. 1996
    ..As a step toward achieving this goal, we communicate the cDNA and deduced amino acid sequence of the monkey fertilin beta subunit...
  10. pmc Molecular cloning of the human and monkey sperm surface protein PH-20
    Y Lin
    Department of Physiology, University of Connecticut Health Center, Farmington 06030
    Proc Natl Acad Sci U S A 90:10071-5. 1993
    ..4-kb message. Northern blots of tissues other than testis are negative for PH-20, indicating that human PH-20 is testis-specific...
  11. ncbi request reprint Chromosomal assignment of four testis-expressed mouse genes from a new family of transmembrane proteins (ADAMs) involved in cell-cell adhesion and fusion
    C Cho
    Department of Physiology, University of Connecticut Health Center, Farmington, Connecticut, 06030, USA
    Genomics 34:413-7. 1996
    ....
  12. ncbi request reprint Analysis of loss of adhesive function in sperm lacking cyritestin or fertilin beta
    H Nishimura
    Section of Molecular and Cellular Biology, University of California, Davis, California 95616, USA
    Dev Biol 233:204-13. 2001
    ..Because the altered sperm protein expression must be responsible for the fertilization defects, our data suggest new models for the molecular basis of the affected steps in fertilization...
  13. ncbi request reprint ADAM, a widely distributed and developmentally regulated gene family encoding membrane proteins with a disintegrin and metalloprotease domain
    T G Wolfsberg
    Department of Pharmacology, University of California, San Francisco 94143, USA
    Dev Biol 169:378-83. 1995
    ..Thus, the ADAMs are a large, widely expressed, and developmentally regulated family of proteins with multiple potential functions in cell-cell and cell-matrix interactions...
  14. ncbi request reprint Genomic organization of the mouse fertilin beta gene that encodes an ADAM family protein active in sperm-egg fusion
    C Cho
    Section of Molecular and Cellular Biology, University of California, Davis 95616, USA
    Dev Genet 20:320-8. 1997
    ..1995): Cytogenet Cell Genet 68:39-44] showed 12 conserved exon-intron boundaries. In addition, we analyzed the fertilin alpha gene, demonstrating that more than one gene is present in the mouse genome...
  15. pmc Normal fertilization occurs with eggs lacking the integrin alpha6beta1 and is CD9-dependent
    B J Miller
    Department of Molecular and Cellular Biology, University of California Davis, Davis, California 95616, USA
    J Cell Biol 149:1289-96. 2000
    ....
  16. pmc A role for the disintegrin domain of cyritestin, a sperm surface protein belonging to the ADAM family, in mouse sperm-egg plasma membrane adhesion and fusion
    R Yuan
    Molecular and Cellular Biology, University of California, Davis 95616, USA
    J Cell Biol 137:105-12. 1997
    ..Collectively, these data indicate that a second ADAM family member, cyritestin, functions with fertilin beta in sperm-egg plasma membrane adhesion leading to fusion...
  17. ncbi request reprint Testase 1 (ADAM 24) a plasma membrane-anchored sperm protease implicated in sperm function during epididymal maturation or fertilization
    G Z Zhu
    Dept of Cell Biology and Human Anatomy, School of Medicine, University of California Davis, Davis, CA 95616, USA
    J Cell Sci 114:1787-94. 2001
    ..Because events of the acrosome reaction include PKC activation, we speculate that testase 1 protease function could be important in sperm penetration of the zona pellucida after sperm PKC is activated during the acrosome reaction...
  18. ncbi request reprint Analysis of mouse fertilin in wild-type and fertilin beta(-/-) sperm: evidence for C-terminal modification, alpha/beta dimerization, and lack of essential role of fertilin alpha in sperm-egg fusion
    C Cho
    Section of Molecular and Cellular Biology, University of California at Davis, Davis, California, 95616, USA
    Dev Biol 222:289-95. 2000
    ..The results suggest instead that other gamete surface molecules act to promote membrane fusion and that fertilin's role in gamete fusion is in sperm-egg plasma membrane adhesion...
  19. pmc A hyaluronidase activity of the sperm plasma membrane protein PH-20 enables sperm to penetrate the cumulus cell layer surrounding the egg
    Y Lin
    Department of Physiology, University of Connecticut Health Center, Farmington 06030
    J Cell Biol 125:1157-63. 1994
    ..These results, indicating that PH-20 enables acrosome intact sperm to penetrate the cumulus barrier, reveal a mechanism for cumulus penetration, and thus provide the missing element in the current model...
  20. ncbi request reprint Why did the sperm cross the cumulus? To get to the oocyte. Functions of the sperm surface proteins PH-20 and fertilin in arriving at, and fusing with, the egg
    D G Myles
    Department of Cell Biology and Human Anatomy, University of California Davis, 95616, USA
    Biol Reprod 56:320-7. 1997
    ..In addition to fertilin, other ADAMs are also present on the sperm plasma membrane and may participate with fertilin in sperm-egg fusion...
  21. ncbi request reprint Mouse egg integrin alpha 6 beta 1 functions as a sperm receptor
    E A Almeida
    Department of Cell Biology, University of Virginia, Charlotteville 22908, USA
    Cell 81:1095-104. 1995
    ..Our results indicate a novel role for the integrin alpha 6 beta 1 as a cell-cell adhesion receptor that mediates sperm-egg binding...
  22. ncbi request reprint Sperm-egg fusion: events at the plasma membrane
    Kathryn K Stein
    Section of Molecular and Cellular Biology, School of Medicine, University of California Davis, Davis, CA 95616, USA
    J Cell Sci 117:6269-74. 2004
    ..These discoveries provide a strong starting point for the identification of additional proteins that have roles in sperm-egg fusion...
  23. pmc Direct binding of the ligand PSG17 to CD9 requires a CD9 site essential for sperm-egg fusion
    Diego A Ellerman
    Department of Cell Biology and Human Anatomy, School of Medicine, University of California Davis, Davis, California 95616, USA
    Mol Biol Cell 14:5098-103. 2003
    ..These findings suggest that egg CD9 may function in gamete fusion by binding to a sperm IgSF/CEA subfamily member and such proteins have previously been identified on sperm...
  24. ncbi request reprint Infertility in female mice with an oocyte-specific knockout of GPI-anchored proteins
    Jennifer A Alfieri
    Section of Molecular and Cell Biology, University of California Davis, One Shields Avenue, Davis, CA 95616, USA
    J Cell Sci 116:2149-55. 2003
    ..Other egg GPI-anchored proteins acting in the fusion process can now be investigated, with the goal of understanding the mechanism of their function in sperm-egg fusion...
  25. doi request reprint Loss of surface EWI-2 on CD9 null oocytes
    Zhi Yong He
    Department of Molecular and Cellular Biology, College of Biological Sciences, University of California Davis, Davis, California 95616, USA
    Mol Reprod Dev 76:629-36. 2009
    ..Overall, the results indicate that deletion of CD9, which is known to have multiple associations, may have pleiotropic effects on function that will require further dissection...
  26. pmc ADAM, a novel family of membrane proteins containing A Disintegrin And Metalloprotease domain: multipotential functions in cell-cell and cell-matrix interactions
    T G Wolfsberg
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143, USA
    J Cell Biol 131:275-8. 1995
  27. pmc cDNA cloning reveals the molecular structure of a sperm surface protein, PH-20, involved in sperm-egg adhesion and the wide distribution of its gene among mammals
    W F Lathrop
    Department of Physiology, University of Connecticut Health Center, Farmington 06032
    J Cell Biol 111:2939-49. 1990
    ..Species specificity may result from limited changes in these molecules, either in their binding domains or in other regions that affect the ability of the binding domains to interact...
  28. ncbi request reprint None of the integrins known to be present on the mouse egg or to be ADAM receptors are essential for sperm-egg binding and fusion
    Zhi Yong He
    Section of Molecular and Cellular Biology, University of California Davis, Davis, CA 95616, USA
    Dev Biol 254:226-37. 2003
    ....
  29. ncbi request reprint Residues SFQ (173-175) in the large extracellular loop of CD9 are required for gamete fusion
    Guo Zhang Zhu
    Department of Cell Biology and Human Anatomy, School of Medicine, University of California Davis, Davis, CA 95616, USA
    Development 129:1995-2002. 2002
    ..These results suggest that SFQ in the CD9 large extracellular loop may be an active site which associates with and regulates the egg fusion machinery...
  30. ncbi request reprint A role for sperm surface protein disulfide isomerase activity in gamete fusion: evidence for the participation of ERp57
    Diego A Ellerman
    Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Davis, Davis, California 95616, USA
    Dev Cell 10:831-7. 2006
    ..The results support the idea that thiol-disulfide exchange is a mechanism that may act in gamete fusion to produce conformational changes in fusion-active proteins...
  31. ncbi request reprint Identification of an ADAM2-ADAM3 complex on the surface of mouse testicular germ cells and cauda epididymal sperm
    Hitoshi Nishimura
    Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Davis, California 95618, USA
    J Biol Chem 282:17900-7. 2007
    ..The presence of the ADAM2-ADAM3 complex in sperm also suggests a potential role of ADAM2 with ADAM3 in sperm binding to the egg zona pellucida...
  32. doi request reprint Testase 1 (ADAM 24) a sperm surface metalloprotease is required for normal fertility in mice
    Guo Zhang Zhu
    Department of Molecular and Cellular Biology, College of Biological Sciences, University of California Davis, Davis, California 95616, USA
    Mol Reprod Dev 76:1106-14. 2009
    ..These findings suggest that testase 1 is a sperm plasma membrane component which contributes to the prevention of polyspermy at the level of the oocyte plasma membrane...
  33. ncbi request reprint Proteomic analysis of sperm regions that mediate sperm-egg interactions
    Kathryn K Stein
    Section of Molecular and Cellular Biology, University of California, Davis, 95616, USA
    Proteomics 6:3533-43. 2006
    ..We have shown that at least two of these novel proteins are localized to the sperm head. In summary, we have identified over 100 proteins that are expressed on mature sperm at the site of sperm-oocyte interactions...
  34. ncbi request reprint Defects in secretory pathway trafficking during sperm development in Adam2 knockout mice
    Kathryn K Stein
    Section of Molecular and Cellular Biology, University of California, Davis, 95616, USA
    Biol Reprod 73:1032-8. 2005
    ..Thus, it appears that cyritestin loss in Adam2-knockout mice may result, at least in part, from a disruption in protein trafficking...
  35. pmc Izumo is part of a multiprotein family whose members form large complexes on mammalian sperm
    Diego A Ellerman
    Department of Cellular and Molecular Biology, University of California, Davis, California 95616, USA
    Mol Reprod Dev 76:1188-99. 2009
    ..Our results raise the possibility that Izumo 1 might be involved in organizing or stabilizing a multiprotein complex essential for the function of the membrane fusion machinery...

Research Grants1

  1. CENTER FOR DEVELOPMENT OF ANTI-SPERM CONTRACEPTIVES
    Paul Primakoff; Fiscal Year: 2006
    ..Thus, we have proposed an expansion of our target list in spermatogenesis, increasing our scope and chances of developing new products for birth control. ..