Mark N Prichard

Summary

Affiliation: University of Alabama at Birmingham
Country: USA

Publications

  1. pmc Inhibition of herpesvirus replication by 5-substituted 4'-thiopyrimidine nucleosides
    Mark N Prichard
    Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL 35233, USA
    Antimicrob Agents Chemother 53:5251-8. 2009
  2. ncbi request reprint Synthesis and antiviral activity of 7-deazaneplanocin A against orthopoxviruses (vaccinia and cowpox virus)
    Balakumar Arumugham
    University of Georgia College of Pharmacy, Athens, GA 30602, USA
    Bioorg Med Chem Lett 16:285-7. 2006
  3. pmc Orthopoxvirus targets for the development of new antiviral agents
    Mark N Prichard
    Department of Pediatrics, The University of Alabama at Birmingham, Birmingham, AL 35233 1711, United States
    Antiviral Res 94:111-25. 2012
  4. pmc Isolation and characterization of cidofovir resistant vaccinia viruses
    Marie N Becker
    University of Florida, Gainesville, FL, USA
    Virol J 5:58. 2008
  5. pmc Function of human cytomegalovirus UL97 kinase in viral infection and its inhibition by maribavir
    Mark N Prichard
    Department of Pediatrics, University of Alabama School of Medicine, Birmingham, Alabama 35233, USA
    Rev Med Virol 19:215-29. 2009
  6. pmc Selective phosphorylation of antiviral drugs by vaccinia virus thymidine kinase
    Mark N Prichard
    Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL 35233, USA
    Antimicrob Agents Chemother 51:1795-803. 2007
  7. pmc A rapid DNA hybridization assay for the evaluation of antiviral compounds against Epstein-Barr virus
    Mark N Prichard
    Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL 35233, USA
    J Virol Methods 144:86-90. 2007
  8. pmc The search for new therapies for human cytomegalovirus infections
    Mark N Prichard
    Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL 35233 1711, USA
    Virus Res 157:212-21. 2011
  9. pmc Vaccinia virus lacking the deoxyuridine triphosphatase gene (F2L) replicates well in vitro and in vivo, but is hypersensitive to the antiviral drug (N)-methanocarbathymidine
    Mark N Prichard
    Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL 35233, USA
    Virol J 5:39. 2008
  10. pmc Human cytomegalovirus UL97 kinase activity is required for the hyperphosphorylation of retinoblastoma protein and inhibits the formation of nuclear aggresomes
    Mark N Prichard
    Department of Pediatrics, University of Alabama School of Medicine, Birmingham, Alabama 35233, USA
    J Virol 82:5054-67. 2008

Research Grants

  1. DEVELOPMENT OF A LIVE ATTENUATED HSV-2 VACCINE
    Mark Prichard; Fiscal Year: 2002

Detail Information

Publications38

  1. pmc Inhibition of herpesvirus replication by 5-substituted 4'-thiopyrimidine nucleosides
    Mark N Prichard
    Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL 35233, USA
    Antimicrob Agents Chemother 53:5251-8. 2009
    ....
  2. ncbi request reprint Synthesis and antiviral activity of 7-deazaneplanocin A against orthopoxviruses (vaccinia and cowpox virus)
    Balakumar Arumugham
    University of Georgia College of Pharmacy, Athens, GA 30602, USA
    Bioorg Med Chem Lett 16:285-7. 2006
    ..The synthesized 7-deazaneplanocin A (2) exhibited potent antiviral activity against cowpox and vaccinia viruses without cytotoxicity in HFF cells...
  3. pmc Orthopoxvirus targets for the development of new antiviral agents
    Mark N Prichard
    Department of Pediatrics, The University of Alabama at Birmingham, Birmingham, AL 35233 1711, United States
    Antiviral Res 94:111-25. 2012
    ..Progress that has been achieved in recent years should yield new drugs for the treatment of these infections and might also reveal new approaches for antiviral drug development with other viruses...
  4. pmc Isolation and characterization of cidofovir resistant vaccinia viruses
    Marie N Becker
    University of Florida, Gainesville, FL, USA
    Virol J 5:58. 2008
    ..One mode of action for CDV has been demonstrated to be the inhibition of the viral DNA polymerase...
  5. pmc Function of human cytomegalovirus UL97 kinase in viral infection and its inhibition by maribavir
    Mark N Prichard
    Department of Pediatrics, University of Alabama School of Medicine, Birmingham, Alabama 35233, USA
    Rev Med Virol 19:215-29. 2009
    ..The critical functions performed by the UL97 kinase illustrate its importance in viral replication and confirm that the kinase is a target for the development of antiviral therapies...
  6. pmc Selective phosphorylation of antiviral drugs by vaccinia virus thymidine kinase
    Mark N Prichard
    Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL 35233, USA
    Antimicrob Agents Chemother 51:1795-803. 2007
    ..This unexpected result suggests that selective phosphorylation by the viral kinase may be a promising new approach in the discovery of highly selective inhibitors of orthopoxvirus replication...
  7. pmc A rapid DNA hybridization assay for the evaluation of antiviral compounds against Epstein-Barr virus
    Mark N Prichard
    Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL 35233, USA
    J Virol Methods 144:86-90. 2007
    ..Subsequent experience with this assay has confirmed that it offers improved efficiency and robustness compared to other assays used routinely to evaluate candidate compounds for antiviral activity against EBV...
  8. pmc The search for new therapies for human cytomegalovirus infections
    Mark N Prichard
    Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL 35233 1711, USA
    Virus Res 157:212-21. 2011
    ..Their antiviral activity both in vitro and in vivo, spectrum of antiviral activity, and mechanism of action will be reviewed to provide an update on the progress of potential new therapies for CMV infections...
  9. pmc Vaccinia virus lacking the deoxyuridine triphosphatase gene (F2L) replicates well in vitro and in vivo, but is hypersensitive to the antiviral drug (N)-methanocarbathymidine
    Mark N Prichard
    Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL 35233, USA
    Virol J 5:39. 2008
    ..Although the crystal structure for this enzyme has been determined, its potential as a target for antiviral therapy is unclear...
  10. pmc Human cytomegalovirus UL97 kinase activity is required for the hyperphosphorylation of retinoblastoma protein and inhibits the formation of nuclear aggresomes
    Mark N Prichard
    Department of Pediatrics, University of Alabama School of Medicine, Birmingham, Alabama 35233, USA
    J Virol 82:5054-67. 2008
    ..These data also suggest a potential link between RB function and the formation of aggresomes...
  11. pmc Inhibition of herpesvirus replication by hexadecyloxypropyl esters of purine- and pyrimidine-based phosphonomethoxyethyl nucleoside phosphonates
    Mark N Prichard
    Department of Pediatrics, University of Alabama School of Medicine, 128 Children s Harbor Building, 1600 6th Ave South, Birmingham, AL 35233, USA
    Antimicrob Agents Chemother 52:4326-30. 2008
    ..The results presented here indicate that HDP-esterified acyclic nucleoside phosphonates with antiviral activity against HIV also inhibit the replication of some herpesviruses and can extend the spectrum of activity for these compounds...
  12. pmc Benzimidazole analogs inhibit human herpesvirus 6
    Mark N Prichard
    University of Alabama School of Medicine, 1600 6th Avenue South, 128 Children s Harbor Building, Birmingham, AL 35233, USA
    Antimicrob Agents Chemother 55:2442-5. 2011
    ..Additional analogs of these compounds were evaluated against both variants of HHV-6, and two L-analogs of BDCRB had good antiviral activity against HHV-6A, as well as more modest inhibition of HHV-6B replication...
  13. pmc Human cytomegalovirus UL27 is not required for viral replication in human tissue implanted in SCID mice
    Mark N Prichard
    Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL, USA
    Virol J 3:18. 2006
    ..These data are consistent with minimal defects observed in cell culture, but are not consistent with an important role for UL27 in vivo. We conclude that UL27 is not required for viral replication in vivo...
  14. pmc Activity and mechanism of action of N-methanocarbathymidine against herpesvirus and orthopoxvirus infections
    Mark N Prichard
    Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL 35233, USA
    Antimicrob Agents Chemother 50:1336-41. 2006
    ..These results indicate that (N)-MCT is active in vitro and in vivo, and its mechanism of action suggests that the molecule may be an effective therapeutic for orthopoxvirus and herpesvirus infections, thus warranting further development...
  15. ncbi request reprint Distinct thymidine kinases encoded by cowpox virus and herpes simplex virus contribute significantly to the differential antiviral activity of nucleoside analogs
    Mark N Prichard
    University of Alabama School of Medicine, Department of Pediatrics, Birmingham, 35233, USA
    Antiviral Res 71:1-6. 2006
    ..Additional screening against orthopoxviruses will be required to identify nucleoside analogs that are efficiently activated by their type II TK...
  16. pmc Human cytomegalovirus UL97 Kinase is required for the normal intranuclear distribution of pp65 and virion morphogenesis
    Mark N Prichard
    Department of Pediatrics, University of Alabama School of Medicine, Birmingham, Alabama 35233, USA
    J Virol 79:15494-502. 2005
    ..We propose that the kinase plays an important role in the acquisition of tegument during virion morphogenesis in the nucleus and that this activity represents an important step in the production of mature virus particles...
  17. pmc CMX001 potentiates the efficacy of acyclovir in herpes simplex virus infections
    Mark N Prichard
    University of Alabama at Birmingham, Department of Pediatrics, 128 Children s Harbor Building, 1600 6th Avenue South, Birmingham, AL 35233 1711, USA
    Antimicrob Agents Chemother 55:4728-34. 2011
    ..These results suggest that CMX001 may be effective in the treatment of ACV-resistant HSV infections and as an adjunct therapy in individuals with suboptimal responses to ACV...
  18. pmc Human cytomegalovirus uracil DNA glycosylase associates with ppUL44 and accelerates the accumulation of viral DNA
    Mark N Prichard
    Department of Pediatrics, University of Alabama, Birmingham, AL, USA
    Virol J 2:55. 2005
    ..The gene product, pUL114, also appears to be important in late phase DNA synthesis presumably by introducing single stranded breaks...
  19. pmc Comparative activities of lipid esters of cidofovir and cyclic cidofovir against replication of herpesviruses in vitro
    Stephanie L Williams-Aziz
    University of Alabama School of Medicine, 1600 6th Ave South, 128 Children s Harbor Bldg, Birmingham, AL 35233, USA
    Antimicrob Agents Chemother 49:3724-33. 2005
    ....
  20. pmc Activities of certain 5-substituted 4'-thiopyrimidine nucleosides against orthopoxvirus infections
    Earl R Kern
    Department of Pediatrics, University of Alabama School of Medicine, Birmingham, 35233, USA
    Antimicrob Agents Chemother 53:572-9. 2009
    ....
  21. pmc Synergistic efficacy of the combination of ST-246 with CMX001 against orthopoxviruses
    Debra C Quenelle
    Department of Pediatrics, The University of Alabama at Birmingham, Birmingham, AL 35233 1711, USA
    Antimicrob Agents Chemother 51:4118-24. 2007
    ....
  22. pmc Cyclopropavir inhibits the normal function of the human cytomegalovirus UL97 kinase
    Scott H James
    University of Alabama at Birmingham, Department of Pediatrics, 128 Children s Harbor Building, 1600 6th Avenue South, Birmingham, AL 35233 1711, USA
    Antimicrob Agents Chemother 55:4682-91. 2011
    ..We conclude that the mechanism of action of CPV against CMV is complex and involves both the inhibition of DNA synthesis and the inhibition of the normal activity of the UL97 kinase...
  23. pmc Conserved retinoblastoma protein-binding motif in human cytomegalovirus UL97 kinase minimally impacts viral replication but affects susceptibility to maribavir
    Rachel B Gill
    Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL, USA
    Virol J 6:9. 2009
    ..Mutation of the amino terminal motif, which is involved in the inactivation of Rb, also renders the virus hypersensitive to the drug and suggests that the motif may play a role in its mechanism of action...
  24. pmc Virucidal activity of polysaccharide extracts from four algal species against herpes simplex virus
    Emma A Harden
    Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL 35233 USA
    Antiviral Res 83:282-9. 2009
    ..We conclude that these extracts are nontoxic and effective virucidal agents that warrant further investigation to examine their potential role in the prevention of HSV infections of humans...
  25. pmc Identification of protein-protein interaction inhibitors targeting vaccinia virus processivity factor for development of antiviral agents
    Norbert Schormann
    Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Antimicrob Agents Chemother 55:5054-62. 2011
    ..These results suggest that novel antiviral agents that target the protein-protein interactions between D4 and A20 can be developed for the treatment of infections with poxviruses, including smallpox...
  26. pmc In vitro activity and mechanism of action of methylenecyclopropane analogs of nucleosides against herpesvirus replication
    Earl R Kern
    The University of Alabama at Birmingham, Department of Pediatrics, 128 Children s Harbor Building, 1600 6th Ave South, Birmingham, AL 35233, USA
    Antimicrob Agents Chemother 49:1039-45. 2005
    ..These results indicate that at least one of these compounds may have potential for use in treating CMV and other herpesvirus infections in humans...
  27. pmc Synthesis and Antiviral Activities of Methylenecyclopropane Analogs with 6-Alkoxy and 6-Alkylthio Substitutions That Exhibit Broad-Spectrum Antiviral Activity against Human Herpesviruses
    Mark N Prichard
    University of Alabama at Birmingham, Birmingham, Alabama, USA
    Antimicrob Agents Chemother 57:3518-27. 2013
    ..They also suggest that they might be useful as broad-spectrum antiherpesvirus agents and may be effective in the treatment of resistant virus infections. ..
  28. doi request reprint Acyclovir-resistant chronic verrucous vaccine strain varicella in a patient with neuroblastoma
    Coleman J Bryan
    Division of Pediatric Hematology Oncology, University of Alabama at Birmingham, Birmingham, AL, USA
    Pediatr Infect Dis J 27:946-8. 2008
    ..After foscarnet therapy, she underwent stem cell transplantation without varicella reactivation. This is only the second reported case of resistant varicella zoster virus caused by Oka strain virus...
  29. pmc Selection and recombinant phenotyping of a novel CMX001 and cidofovir resistance mutation in human cytomegalovirus
    Scott H James
    University of Alabama at Birmingham, Birmingham, Alabama, USA
    Antimicrob Agents Chemother 57:3321-5. 2013
    ....
  30. pmc Human cytomegalovirus UL97 kinase alters the accumulation of CDK1
    Rachel B Gill
    Department of Cell Biology, 1900 University Blvd, Birmingham, AL 35294, USA
    J Gen Virol 93:1743-55. 2012
    ..These results suggest that, in addition to phosphorylating CDK1 targets, the UL97 kinase modifies G₂/M cell-cycle checkpoint regulators, specifically CDK1, to promote virus replication...
  31. pmc Progress in the development of new therapies for herpesvirus infections
    Nathan B Price
    Department of Pediatrics, University of Alabama, School of Medicine, Birmingham, AL 35233 1711, USA
    Curr Opin Virol 1:548-54. 2011
    ..Recent advances with each of these compounds will be reviewed including their virus specificity, mechanism of action, and stage of development. The potential of these new compounds to improve clinical outcome will also be discussed...
  32. ncbi request reprint Orthopoxvirus targets for the development of antiviral therapies
    Mark N Prichard
    University of Alabama School of Medicine, Department of Pediatrics, 1600 6th Avenue South, Birmingham, AL 35233, USA
    Curr Drug Targets Infect Disord 5:17-28. 2005
    ..The rapid progress that has been achieved in recent years should yield new drugs for the treatment of these infections and might also reveal new strategies for antiviral therapy with other viruses...
  33. pmc Effect of an immune enhancer, GPI-0100, on vaccination with live attenuated herpes simplex virus (HSV) type 2 or glycoprotein D on genital HSV-2 infections of guinea pigs
    Debra C Quenelle
    University of Alabama at Birmingham, Department of Pediatrics, Birmingham, AL 35233 1711, USA
    Antiviral Res 80:223-4. 2008
    ..Neither vaccine had any therapeutic effect if administered 24 h after viral infection...
  34. pmc Antiviral Activity of 4'-thioIDU and Thymidine Analogs against Orthopoxviruses
    Mark N Prichard
    Department of Pediatrics, University of Alabama School of Medicine, CHB 128, 1600 6th Ave S, Birmingham AL 35233 1711, USA E mail
    Viruses 2:1968-83. 2010
    ..The latter molecule has proven to have good antiviral activity against the orthopoxviruses both in vitro and in vivo and has the potential to be an effective therapy in humans...
  35. ncbi request reprint Toward orthopoxvirus countermeasures: a novel heteromorphic nucleoside of unusual structure
    Xuesen Fan
    Department of Chemistry and Biochemistry, Northern Arizona University, Flagstaff, 86011, USA
    J Med Chem 49:4052-4. 2006
    ..Compound 2 was efficiently phosphorylated by VV TK, establishing that VV TK is more promiscuous than previously believed...
  36. pmc Antiviral activities of novel 5-phosphono-pent-2-en-1-yl nucleosides and their alkoxyalkyl phosphonoesters
    Hyunah Choo
    Department of Medicine 0676, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0676, USA
    Antimicrob Agents Chemother 51:611-5. 2007
    ..Notably, the hexadecyloxypropyl ester of 9-(5-phosphono-pent-2-en-1-yl)-adenine was active against hepatitis B virus mutants resistant to lamivudine, emtricitabine, and adefovir...
  37. pmc Evaluation of AD472, a live attenuated recombinant herpes simplex virus type 2 vaccine in guinea pigs
    Mark N Prichard
    Medlmmune Vaccines Inc, 297 N Bernardo Avenue, Mountain View, CA 94043, USA
    Vaccine 23:5424-31. 2005
    ..Results presented here support the concept of a rationally engineered live attenuated vaccine for the prevention of the genital disease associated with infection by HSV-2...
  38. pmc Fluorinated methylenecyclopropane analogues of nucleosides. Synthesis and antiviral activity of (Z)- and (E)-9-{[(2-fluoromethyl-2-hydroxymethyl)-cyclopropylidene]methyl}adenine and -guanine
    Chengwei Li
    Developmental Therapeutics Program, Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, MI 48201 1379, USA
    Bioorg Med Chem 16:2148-55. 2008
    ..The E-isomer 16a was a substrate for adenosine deaminase, whereas the Z-isomer 15a was not deaminated...

Research Grants1

  1. DEVELOPMENT OF A LIVE ATTENUATED HSV-2 VACCINE
    Mark Prichard; Fiscal Year: 2002
    ..In addition further in vitro characterization will enable high quality clinical trial material to be produced for IND enabling primate studies and early Phase I human trials. PROPOSED COMMERCIAL APPLICATION: Not Available ..