Research Topics
| Mark N PrichardSummaryAffiliation: University of Alabama at Birmingham Country: USA Publications
Research Grants
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Detail Information
Publications
Synthesis and antiviral activity of 7-deazaneplanocin A against orthopoxviruses (vaccinia and cowpox virus)Balakumar Arumugham
University of Georgia College of Pharmacy, Athens, GA 30602, USA
Bioorg Med Chem Lett 16:285-7. 2006..The synthesized 7-deazaneplanocin A (2) exhibited potent antiviral activity against cowpox and vaccinia viruses without cytotoxicity in HFF cells...
Inhibition of herpesvirus replication by 5-substituted 4'-thiopyrimidine nucleosidesMark N Prichard
Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL 35233, USA
Antimicrob Agents Chemother 53:5251-8. 2009....
Orthopoxvirus targets for the development of new antiviral agentsMark N Prichard
Department of Pediatrics, The University of Alabama at Birmingham, Birmingham, AL 35233 1711, United States
Antiviral Res 94:111-25. 2012..Progress that has been achieved in recent years should yield new drugs for the treatment of these infections and might also reveal new approaches for antiviral drug development with other viruses...- Isolation and characterization of cidofovir resistant vaccinia virusesMarie N Becker
University of Florida, Gainesville, FL, USA
Virol J 5:58. 2008..One mode of action for CDV has been demonstrated to be the inhibition of the viral DNA polymerase... - Selective phosphorylation of antiviral drugs by vaccinia virus thymidine kinaseMark N Prichard
Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL 35233, USA
Antimicrob Agents Chemother 51:1795-803. 2007..This unexpected result suggests that selective phosphorylation by the viral kinase may be a promising new approach in the discovery of highly selective inhibitors of orthopoxvirus replication... - A rapid DNA hybridization assay for the evaluation of antiviral compounds against Epstein-Barr virusMark N Prichard
Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL 35233, USA
J Virol Methods 144:86-90. 2007..Subsequent experience with this assay has confirmed that it offers improved efficiency and robustness compared to other assays used routinely to evaluate candidate compounds for antiviral activity against EBV... - The search for new therapies for human cytomegalovirus infectionsMark N Prichard
Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL 35233 1711, USA
Virus Res 157:212-21. 2011..Their antiviral activity both in vitro and in vivo, spectrum of antiviral activity, and mechanism of action will be reviewed to provide an update on the progress of potential new therapies for CMV infections... - Vaccinia virus lacking the deoxyuridine triphosphatase gene (F2L) replicates well in vitro and in vivo, but is hypersensitive to the antiviral drug (N)-methanocarbathymidineMark N Prichard
Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL 35233, USA
Virol J 5:39. 2008..Although the crystal structure for this enzyme has been determined, its potential as a target for antiviral therapy is unclear... - Human cytomegalovirus UL97 kinase activity is required for the hyperphosphorylation of retinoblastoma protein and inhibits the formation of nuclear aggresomesMark N Prichard
Department of Pediatrics, University of Alabama School of Medicine, Birmingham, Alabama 35233, USA
J Virol 82:5054-67. 2008..These data also suggest a potential link between RB function and the formation of aggresomes... - Function of human cytomegalovirus UL97 kinase in viral infection and its inhibition by maribavirMark N Prichard
Department of Pediatrics, University of Alabama School of Medicine, Birmingham, Alabama 35233, USA
Rev Med Virol 19:215-29. 2009..The critical functions performed by the UL97 kinase illustrate its importance in viral replication and confirm that the kinase is a target for the development of antiviral therapies... - Inhibition of herpesvirus replication by hexadecyloxypropyl esters of purine- and pyrimidine-based phosphonomethoxyethyl nucleoside phosphonatesMark N Prichard
Department of Pediatrics, University of Alabama School of Medicine, 128 Children s Harbor Building, 1600 6th Ave South, Birmingham, AL 35233, USA
Antimicrob Agents Chemother 52:4326-30. 2008..The results presented here indicate that HDP-esterified acyclic nucleoside phosphonates with antiviral activity against HIV also inhibit the replication of some herpesviruses and can extend the spectrum of activity for these compounds... - Benzimidazole analogs inhibit human herpesvirus 6Mark N Prichard
University of Alabama School of Medicine, 1600 6th Avenue South, 128 Children s Harbor Building, Birmingham, AL 35233, USA
Antimicrob Agents Chemother 55:2442-5. 2011..Additional analogs of these compounds were evaluated against both variants of HHV-6, and two L-analogs of BDCRB had good antiviral activity against HHV-6A, as well as more modest inhibition of HHV-6B replication... - CMX001 potentiates the efficacy of acyclovir in herpes simplex virus infectionsMark N Prichard
University of Alabama at Birmingham, Department of Pediatrics, 128 Children s Harbor Building, 1600 6th Avenue South, Birmingham, AL 35233 1711, USA
Antimicrob Agents Chemother 55:4728-34. 2011..These results suggest that CMX001 may be effective in the treatment of ACV-resistant HSV infections and as an adjunct therapy in individuals with suboptimal responses to ACV... - Human cytomegalovirus UL27 is not required for viral replication in human tissue implanted in SCID miceMark N Prichard
Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL, USA
Virol J 3:18. 2006..These data are consistent with minimal defects observed in cell culture, but are not consistent with an important role for UL27 in vivo. We conclude that UL27 is not required for viral replication in vivo... - Activity and mechanism of action of N-methanocarbathymidine against herpesvirus and orthopoxvirus infectionsMark N Prichard
Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL 35233, USA
Antimicrob Agents Chemother 50:1336-41. 2006..These results indicate that (N)-MCT is active in vitro and in vivo, and its mechanism of action suggests that the molecule may be an effective therapeutic for orthopoxvirus and herpesvirus infections, thus warranting further development... - Distinct thymidine kinases encoded by cowpox virus and herpes simplex virus contribute significantly to the differential antiviral activity of nucleoside analogsMark N Prichard
University of Alabama School of Medicine, Department of Pediatrics, Birmingham, 35233, USA
Antiviral Res 71:1-6. 2006..Additional screening against orthopoxviruses will be required to identify nucleoside analogs that are efficiently activated by their type II TK... - Human cytomegalovirus UL97 Kinase is required for the normal intranuclear distribution of pp65 and virion morphogenesisMark N Prichard
Department of Pediatrics, University of Alabama School of Medicine, Birmingham, Alabama 35233, USA
J Virol 79:15494-502. 2005..We propose that the kinase plays an important role in the acquisition of tegument during virion morphogenesis in the nucleus and that this activity represents an important step in the production of mature virus particles... - Human cytomegalovirus uracil DNA glycosylase associates with ppUL44 and accelerates the accumulation of viral DNAMark N Prichard
Department of Pediatrics, University of Alabama, Birmingham, AL, USA
Virol J 2:55. 2005..The gene product, pUL114, also appears to be important in late phase DNA synthesis presumably by introducing single stranded breaks... - Comparative activities of lipid esters of cidofovir and cyclic cidofovir against replication of herpesviruses in vitroStephanie L Williams-Aziz
University of Alabama School of Medicine, 1600 6th Ave. South, 128 Children's Harbor Bldg, Birmingham, AL 35233, USA
Antimicrob Agents Chemother 49:3724-33. 2005.... - Activities of certain 5-substituted 4'-thiopyrimidine nucleosides against orthopoxvirus infectionsEarl R Kern
Department of Pediatrics, University of Alabama School of Medicine, Birmingham, 35233, USA
Antimicrob Agents Chemother 53:572-9. 2009.... - Synergistic efficacy of the combination of ST-246 with CMX001 against orthopoxvirusesDebra C Quenelle
Department of Pediatrics, The University of Alabama at Birmingham, Birmingham, AL 35233 1711, USA
Antimicrob Agents Chemother 51:4118-24. 2007.... - Cyclopropavir inhibits the normal function of the human cytomegalovirus UL97 kinaseScott H James
University of Alabama at Birmingham, Department of Pediatrics, 128 Children s Harbor Building, 1600 6th Avenue South, Birmingham, AL 35233 1711, USA
Antimicrob Agents Chemother 55:4682-91. 2011..We conclude that the mechanism of action of CPV against CMV is complex and involves both the inhibition of DNA synthesis and the inhibition of the normal activity of the UL97 kinase... - Conserved retinoblastoma protein-binding motif in human cytomegalovirus UL97 kinase minimally impacts viral replication but affects susceptibility to maribavirRachel B Gill
Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL, USA
Virol J 6:9. 2009..Mutation of the amino terminal motif, which is involved in the inactivation of Rb, also renders the virus hypersensitive to the drug and suggests that the motif may play a role in its mechanism of action... - Virucidal activity of polysaccharide extracts from four algal species against herpes simplex virusEmma A Harden
Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL 35233 USA
Antiviral Res 83:282-9. 2009..We conclude that these extracts are nontoxic and effective virucidal agents that warrant further investigation to examine their potential role in the prevention of HSV infections of humans... - Identification of protein-protein interaction inhibitors targeting vaccinia virus processivity factor for development of antiviral agentsNorbert Schormann
Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
Antimicrob Agents Chemother 55:5054-62. 2011..These results suggest that novel antiviral agents that target the protein-protein interactions between D4 and A20 can be developed for the treatment of infections with poxviruses, including smallpox... - In vitro activity and mechanism of action of methylenecyclopropane analogs of nucleosides against herpesvirus replicationEarl R Kern
The University of Alabama at Birmingham, Department of Pediatrics, 128 Children s Harbor Building, 1600 6th Ave South, Birmingham, AL 35233, USA
Antimicrob Agents Chemother 49:1039-45. 2005..These results indicate that at least one of these compounds may have potential for use in treating CMV and other herpesvirus infections in humans... - Acyclovir-resistant chronic verrucous vaccine strain varicella in a patient with neuroblastomaColeman J Bryan
Division of Pediatric Hematology Oncology, University of Alabama at Birmingham, Birmingham, AL, USA
Pediatr Infect Dis J 27:946-8. 2008..After foscarnet therapy, she underwent stem cell transplantation without varicella reactivation. This is only the second reported case of resistant varicella zoster virus caused by Oka strain virus... - Human cytomegalovirus UL97 kinase alters the accumulation of CDK1Rachel B Gill
Department of Cell Biology, 1900 University Blvd, Birmingham, AL 35294, USA
J Gen Virol 93:1743-55. 2012..These results suggest that, in addition to phosphorylating CDK1 targets, the UL97 kinase modifies G₂/M cell-cycle checkpoint regulators, specifically CDK1, to promote virus replication... - Progress in the development of new therapies for herpesvirus infectionsNathan B Price
Department of Pediatrics, University of Alabama, School of Medicine, Birmingham, AL 35233 1711, USA
Curr Opin Virol 1:548-54. 2011..Recent advances with each of these compounds will be reviewed including their virus specificity, mechanism of action, and stage of development. The potential of these new compounds to improve clinical outcome will also be discussed... - Effect of an immune enhancer, GPI-0100, on vaccination with live attenuated herpes simplex virus (HSV) type 2 or glycoprotein D on genital HSV-2 infections of guinea pigsDebra C Quenelle
University of Alabama at Birmingham, Department of Pediatrics, Birmingham, AL 35233 1711, USA
Antiviral Res 80:223-4. 2008..Neither vaccine had any therapeutic effect if administered 24 h after viral infection... - Orthopoxvirus targets for the development of antiviral therapiesMark N Prichard
University of Alabama School of Medicine, Department of Pediatrics, 1600 6th Avenue South, Birmingham, AL 35233, USA
Curr Drug Targets Infect Disord 5:17-28. 2005..The rapid progress that has been achieved in recent years should yield new drugs for the treatment of these infections and might also reveal new strategies for antiviral therapy with other viruses... - Antiviral Activity of 4'-thioIDU and Thymidine Analogs against OrthopoxvirusesMark N Prichard
Department of Pediatrics, University of Alabama School of Medicine, CHB 128, 1600 6th Ave S, Birmingham AL 35233 1711, USA E mail
Viruses 2:1968-83. 2010..The latter molecule has proven to have good antiviral activity against the orthopoxviruses both in vitro and in vivo and has the potential to be an effective therapy in humans... - Antiviral activities of novel 5-phosphono-pent-2-en-1-yl nucleosides and their alkoxyalkyl phosphonoestersHyunah Choo
Department of Medicine 0676, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0676, USA
Antimicrob Agents Chemother 51:611-5. 2007..Notably, the hexadecyloxypropyl ester of 9-(5-phosphono-pent-2-en-1-yl)-adenine was active against hepatitis B virus mutants resistant to lamivudine, emtricitabine, and adefovir... - Toward orthopoxvirus countermeasures: a novel heteromorphic nucleoside of unusual structureXuesen Fan
Department of Chemistry and Biochemistry, Northern Arizona University, Flagstaff, 86011, USA
J Med Chem 49:4052-4. 2006..Compound 2 was efficiently phosphorylated by VV TK, establishing that VV TK is more promiscuous than previously believed... - Evaluation of AD472, a live attenuated recombinant herpes simplex virus type 2 vaccine in guinea pigsMark N Prichard
Medlmmune Vaccines Inc, 297 N Bernardo Avenue, Mountain View, CA 94043, USA
Vaccine 23:5424-31. 2005..Results presented here support the concept of a rationally engineered live attenuated vaccine for the prevention of the genital disease associated with infection by HSV-2... - Fluorinated methylenecyclopropane analogues of nucleosides. Synthesis and antiviral activity of (Z)- and (E)-9-{[(2-fluoromethyl-2-hydroxymethyl)-cyclopropylidene]methyl}adenine and -guanineChengwei Li
Developmental Therapeutics Program, Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, MI 48201 1379, USA
Bioorg Med Chem 16:2148-55. 2008..The E-isomer 16a was a substrate for adenosine deaminase, whereas the Z-isomer 15a was not deaminated...
Research Grants
- DEVELOPMENT OF A LIVE ATTENUATED HSV-2 VACCINEMark Prichard; Fiscal Year: 2002..In addition further in vitro characterization will enable high quality clinical trial material to be produced for IND enabling primate studies and early Phase I human trials. PROPOSED COMMERCIAL APPLICATION: Not Available ..