RANDALL PRATHER

Summary

Affiliation: University of Missouri
Country: USA

Publications

  1. ncbi request reprint Cloned transgenic heart-healthy pork?
    Randall S Prather
    Division of Animal Sciences, University of Missouri Columbia, 920 East Campus Drive, E125 Animal Science Research centre, Columbia, MO 65211, USA
    Transgenic Res 15:405-7. 2006
  2. pmc Dickkopf-related protein 1 inhibits the WNT signaling pathway and improves pig oocyte maturation
    Lee D Spate
    Division of Animal Science, Animal Science Research Center, Columbia, Missouri, United States of America
    PLoS ONE 9:e95114. 2014
  3. pmc High-throughput cryopreservation of in vivo-derived swine embryos
    Lee D Spate
    Division of Animal Sciences, University of Missouri, Columbia, Missouri, United States of America
    PLoS ONE 8:e65545. 2013
  4. pmc Xenopus egg extract treatment reduced global DNA methylation of donor cells and enhanced somatic cell nuclear transfer embryo development in pigs
    Xiaoyu Yang
    Division of Animal Sciences, University of Missouri, Columbia, Missouri Key Laboratory of Stem Cell and Regenerative Medicine, Center of Cell Developmental Biology, College of Preclinical Medicine, Fujian Medical University, Fuzhou, P R China
    Biores Open Access 1:79-87. 2012
  5. pmc An intact sialoadhesin (Sn/SIGLEC1/CD169) is not required for attachment/internalization of the porcine reproductive and respiratory syndrome virus
    Randall S Prather
    Division of Animal Science, College of Food Agriculture and Natural Resources, University of Missouri, Columbia, Missouri, USA
    J Virol 87:9538-46. 2013
  6. pmc Completion of the swine genome will simplify the production of swine as a large animal biomedical model
    Eric M Walters
    National Swine Resource and Research Center, University of Missouri, Columbia, MO 65211, USA
    BMC Med Genomics 5:55. 2012
  7. pmc Creating genetically modified pigs by using nuclear transfer
    Liangxue Lai
    Department of Animal Science, University of Missouri Columbia, Columbia, MO 65211, USA
    Reprod Biol Endocrinol 1:82. 2003
  8. pmc Targeted genetic modification: xenotransplantation and beyond
    Randall S Prather
    Division of Animal Science, University of Missouri Columbia, Columbia, Missouri 65211 5300, USA
    Cloning Stem Cells 9:17-20. 2007
  9. ncbi request reprint Changes in the structure of nuclei after transfer to oocytes
    R S Prather
    University of Missouri Columbia, Columbia, MO 65211, USA
    Cloning 2:117-22. 2000
  10. ncbi request reprint Cell cycle analysis of cultured porcine mammary cells
    R S Prather
    Department of Animal Sciences, University of Missouri Columbia, Columbia, Missouri 65211 5300, USA
    Cloning 1:17-24. 1999

Detail Information

Publications90

  1. ncbi request reprint Cloned transgenic heart-healthy pork?
    Randall S Prather
    Division of Animal Sciences, University of Missouri Columbia, 920 East Campus Drive, E125 Animal Science Research centre, Columbia, MO 65211, USA
    Transgenic Res 15:405-7. 2006
    ..The descendants of these hfat-1 transgenic swine will be very useful as models of the human condition, and if they are permitted to enter the food chain, they may improve human health...
  2. pmc Dickkopf-related protein 1 inhibits the WNT signaling pathway and improves pig oocyte maturation
    Lee D Spate
    Division of Animal Science, Animal Science Research Center, Columbia, Missouri, United States of America
    PLoS ONE 9:e95114. 2014
    ..It is concluded that transcriptional profiling is a powerful method for detecting differences between in vitro and in vivo matured oocytes, and that the WNT signaling pathway is important for proper oocyte maturation. ..
  3. pmc High-throughput cryopreservation of in vivo-derived swine embryos
    Lee D Spate
    Division of Animal Sciences, University of Missouri, Columbia, Missouri, United States of America
    PLoS ONE 8:e65545. 2013
    ..While requiring a different base culture medium than in vitro-produced embryos, in vivo-derived embryos also survive cryopreservation without damage to their zona pellucida...
  4. pmc Xenopus egg extract treatment reduced global DNA methylation of donor cells and enhanced somatic cell nuclear transfer embryo development in pigs
    Xiaoyu Yang
    Division of Animal Sciences, University of Missouri, Columbia, Missouri Key Laboratory of Stem Cell and Regenerative Medicine, Center of Cell Developmental Biology, College of Preclinical Medicine, Fujian Medical University, Fuzhou, P R China
    Biores Open Access 1:79-87. 2012
    ..Moreover, the combined treatments of donor cells with egg extract before nuclear transfer and of cloned embryos with scriptaid could improve cloned embryo development additively...
  5. pmc An intact sialoadhesin (Sn/SIGLEC1/CD169) is not required for attachment/internalization of the porcine reproductive and respiratory syndrome virus
    Randall S Prather
    Division of Animal Science, College of Food Agriculture and Natural Resources, University of Missouri, Columbia, Missouri, USA
    J Virol 87:9538-46. 2013
    ..The results demonstrate that the expression of the SIGLEC1 gene is not required for infection of pigs with PRRSV and that the absence of SIGLEC1 does not contribute to the pathogenesis of acute disease. ..
  6. pmc Completion of the swine genome will simplify the production of swine as a large animal biomedical model
    Eric M Walters
    National Swine Resource and Research Center, University of Missouri, Columbia, MO 65211, USA
    BMC Med Genomics 5:55. 2012
    ..Anatomic and physiological similarities to the human make swine an excellent large animal model for human health and disease...
  7. pmc Creating genetically modified pigs by using nuclear transfer
    Liangxue Lai
    Department of Animal Science, University of Missouri Columbia, Columbia, MO 65211, USA
    Reprod Biol Endocrinol 1:82. 2003
    ....
  8. pmc Targeted genetic modification: xenotransplantation and beyond
    Randall S Prather
    Division of Animal Science, University of Missouri Columbia, Columbia, Missouri 65211 5300, USA
    Cloning Stem Cells 9:17-20. 2007
  9. ncbi request reprint Changes in the structure of nuclei after transfer to oocytes
    R S Prather
    University of Missouri Columbia, Columbia, MO 65211, USA
    Cloning 2:117-22. 2000
    ..This paper will focus on the changes to nuclear structure that result from transfer to the cytoplasm of an oocyte, as well as some of the changes in the patterns of RNA synthesis that have been described...
  10. ncbi request reprint Cell cycle analysis of cultured porcine mammary cells
    R S Prather
    Department of Animal Sciences, University of Missouri Columbia, Columbia, Missouri 65211 5300, USA
    Cloning 1:17-24. 1999
    ..It is concluded that many cell cycle synchronization techniques are effective in porcine mammary cell lines, but none of the techniques are 100% effective. Such results should help elucidate the mechanisms involved in nuclear transfer...
  11. ncbi request reprint Nuclear remodeling and reprogramming in transgenic pig production
    Randall S Prather
    Department of Animal Science, University of Missouri at Columbia, Columbia, MO 65211 5300, USA
    Exp Biol Med (Maywood) 229:1120-6. 2004
    ..A better understanding of chromatin remodeling and subsequent developmental gene expression will provide clues as to how procedures can be modified to generate fertile animals more efficiently...
  12. pmc Transcriptional, post-transcriptional and epigenetic control of porcine oocyte maturation and embryogenesis
    R S Prather
    Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USA
    Soc Reprod Fertil Suppl 66:165-76. 2009
    ....
  13. ncbi request reprint Genetically modified pigs for medicine and agriculture
    Randall S Prather
    Division of Animal Science, University of Missouri, Columbia, MO 6521, USA
    Biotechnol Genet Eng Rev 25:245-65. 2008
    ..Additional types of genetic modifications will likely provide animals with characteristics that will benefit humans in currently unimagined ways...
  14. ncbi request reprint Cloning. Pigs is pigs
    R S Prather
    Department of Animal Sciences, University of Missouri Columbia, Columbia, MO 65211, USA
    Science 289:1886-7. 2000
    ..The pig is also a valuable domestic animal to have cloned because, being physiologically close to humans, its organs can be used in xenotransplantation...
  15. ncbi request reprint Basic mechanisms of fertilization and parthenogenesis in pigs
    R S Prather
    Department of Animal Sciences, University of Missouri Columbia, Columbia, MO 65211, USA
    Reprod Suppl 58:105-12. 2001
    ..This review will focus on what is known about fertilization and methods to mimic this process, with an emphasis on pigs...
  16. ncbi request reprint Nuclear remodeling and nuclear reprogramming for making transgenic pigs by nuclear transfer
    Randall S Prather
    Division of Animal Science, Food for the 21st Century, College of Food, Agriculture and Natural Resources, University of Missouri Columbia, 920 East Campus Drive, E125 ASRC, Columbia, Missouri 65211 5300, USA
    Adv Exp Med Biol 591:1-13. 2007
    ..However, even with the current low efficiencies this technique has proved very valuable for the production of animals that might be useful for tissue or organ transplantation to humans...
  17. ncbi request reprint High developmental competence of pig oocytes after meiotic inhibition with a specific M-phase promoting factor kinase inhibitor, butyrolactone I
    Guang ming Wu
    Department of Animal Sciences, University of Missouri Columbia, Columbia, MO 65211, USA
    Biol Reprod 67:170-7. 2002
    ..Butyrolactone I is a potent inhibitor of nuclear maturation of porcine oocytes, and the inhibition is fully reversible...
  18. ncbi request reprint Regulation of mitogen-activated protein kinase phosphorylation, microtubule organization, chromatin behavior, and cell cycle progression by protein phosphatases during pig oocyte maturation and fertilization in vitro
    Qing Yuan Sun
    Department of Veterinary Pathobiology, University of Missouri Columbia, Columbia, Missouri 65211, USA
    Biol Reprod 66:580-8. 2002
    ....
  19. pmc Aberrant DNA methylation in porcine in vitro-, parthenogenetic-, and somatic cell nuclear transfer-produced blastocysts
    Aaron J Bonk
    Division of Animal Science, University of Missouri, Columbia, Missouri 65211, USA
    Mol Reprod Dev 75:250-64. 2008
    ..Instead, the appropriate methylation of a relatively small number of genomic regions in the early embryo may enable early development to occur...
  20. ncbi request reprint The Nuclear Mitotic Apparatus (NuMA) protein is contributed by the donor cell nucleus in cloned porcine embryos
    Zhonghua Liu
    Department of Animal Science, University of Missouri Columbia, Columbia, MO 65211, USA
    Front Biosci 11:1945-57. 2006
    ..These results show that the donor cell nucleus contains NuMA that is contributed to the reconstructed embryo and possibly activated by mechanisms in the oocyte's cytoplast...
  21. pmc Mitochondrial distribution and microtubule organization in fertilized and cloned porcine embryos: implications for developmental potential
    Mika Katayama
    Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri Columbia, MO, USA
    Dev Biol 299:206-20. 2006
    ....
  22. ncbi request reprint Mosaic gene expression in nuclear transfer-derived embryos and the production of cloned transgenic pigs from ear-derived fibroblasts
    Kwang Wook Park
    Department of Animal Sciences, University of Missouri Columbia, 920 East Campus Drive, Columbia, MO 65211, USA
    Biol Reprod 66:1001-5. 2002
    ..In addition, transgene expression in a genetically manipulated embryo may not be an accurate indicator of expression in the resulting offspring...
  23. ncbi request reprint The role of cytoplasmic polyadenylation element sequence on mRNA abundance during porcine embryogenesis and parthenogenetic development
    Kyle B Dobbs
    University of Missouri, Columbia, MO 65211, USA
    Mol Reprod Dev 77:699-709. 2010
    ..Thus, CPEs may not be the only factors that regulate message stability, and parthenogenetic activation does not result in changes in transcript abundance that mimic in vitro fertilization...
  24. doi request reprint Method of oocyte activation affects cloning efficiency in pigs
    Kristin M Whitworth
    Division of Animal Sciences, University of Missouri, Columbia, Missouri 65211, USA
    Mol Reprod Dev 76:490-500. 2009
    ..The beneficial effect of NT2 protocol may be due to the stimulation of proteasomes that infiltrate donor cell nucleus shortly after nuclear transfer...
  25. pmc Optimization of square-wave electroporation for transfection of porcine fetal fibroblasts
    Jason W Ross
    Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USA
    Transgenic Res 19:611-20. 2010
    ..The utilization of these conditions produced numerous transgenic fibroblast colonies following G418 selection that when used for somatic cell nuclear transfer resulted in the production of live offspring...
  26. pmc Correlation of developmental differences of nuclear transfer embryos cells to the methylation profiles of nuclear transfer donor cells in Swine
    Aaron J Bonk
    Division of Animal Science, University of Missouri Columbia, Columbia, Missouri, USA
    Epigenetics 2:179-86. 2007
    ..Our findings show there is an inverse correlation to the similarity of the methylation profiles of the donor cells and the in vivo-produced embryos, and to the blastocyst rates following SCNT...
  27. ncbi request reprint Remodeling of centrosomes in intraspecies and interspecies nuclear transfer porcine embryos
    Zhisheng Zhong
    Dept of Veterinary Pathobiology, University of Missouri Columbia, 65211, USA
    Cell Cycle 6:1510-20. 2007
    ....
  28. ncbi request reprint Birth of piglets by in vitro fertilization of zona-free porcine oocytes
    Guang ming Wu
    Department of Animal Sciences, 159 Animal Science Research Center, College of Agriculture, University of Missouri Columbia, 920 East Campus Drive, Columbia, MO 65211, USA
    Theriogenology 62:1544-56. 2004
    ....
  29. doi request reprint Effect of epigenetic regulation during swine embryogenesis and on cloning by nuclear transfer
    Jianguo Zhao
    National Swine Resource and Research Center, University of Missouri, Columbia, MO 65211, USA
    Cell Tissue Res 341:13-21. 2010
    ..The discussion ends with a description of attempts at assisted remodeling of the donor somatic cell nucleus and the SCNT embryo...
  30. ncbi request reprint Proceedings of the Conference on Swine in Biomedical Research
    John K Critser
    Department of Veterinary Pathobiology, University of Missouri, N132 RADIL, 4011 Discovery Drive, Columbia, MO 65201, USA
    ILAR J 50:89-94. 2009
    ..In addition, a keynote speaker discussed the bioengineering of organ printing. This article presents a synopsis of the proceedings of this conference; abstracts of the presentations are available online (www.nsrrc.missouri.edu)...
  31. pmc Generation of cloned transgenic pigs rich in omega-3 fatty acids
    Liangxue Lai
    Division of Animal Science, University of Missouri Columbia, Columbia, Missouri 65211, USA
    Nat Biotechnol 24:435-6. 2006
    ..The hfat-1 transgenic pigs produce high levels of n-3 fatty acids from n-6 analogs, and their tissues have a significantly reduced ratio of n-6/n-3 fatty acids (P < 0.001)...
  32. ncbi request reprint Nuclear remodeling after SCNT: a contractor's nightmare
    Peter Sutovsky
    Department of Animal Science, University of Missouri Columbia, S141 ASRC, 920 East Campus Drive, Columbia, MO 65211, USA
    Trends Biotechnol 22:205-8. 2004
  33. ncbi request reprint Transgenic pig expressing the enhanced green fluorescent protein produced by nuclear transfer using colchicine-treated fibroblasts as donor cells
    Liangxue Lai
    Department of Animal Sciences, University of Missouri Columbia, Columbia, Missouri 65211, USA
    Mol Reprod Dev 62:300-6. 2002
    ..Furthermore, the tissues expressing EGFP from descendents of this pig may be very useful in future studies using pigs that require genetically marked cells...
  34. ncbi request reprint Multipotent adult progenitor cell lines originating from the peripheral blood of green fluorescent protein transgenic swine
    Elmer M Price
    Dalton Cardiovascular Research Center, University of Missouri Columbia, Columbia, MO 65211, USA
    Stem Cells Dev 15:507-22. 2006
    ..PBD-MAPCs are ideal tools to study the mechanisms of differentiation and may be superior to embryonic stem cells as cellular therapeutics...
  35. ncbi request reprint Enhanced developmental potential of heat-shocked porcine parthenogenetic embryos is related to accelerated mitogen-activated protein kinase dephosphorylation
    S Clay Isom
    Division of Animal Sciences, University of Missouri Columbia, Columbia, MO, USA
    Reprod Fertil Dev 21:892-900. 2009
    ..The faster cleavage divisions observed in HS embryos may be linked physiologically to their enhanced developmental potential in vitro...
  36. ncbi request reprint Cloned transgenic swine via in vitro production and cryopreservation
    Rongfeng Li
    Division of Animal Science, University of Missouri Columbia, Columbia, Missouri 65211, USA
    Biol Reprod 75:226-30. 2006
    ..Application of this technique may permit national and international movement of cloned transgenic swine embryos, storage until a suitable surrogate is available, or the long-term frozen storage of valuable genetics...
  37. pmc Significant improvement in cloning efficiency of an inbred miniature pig by histone deacetylase inhibitor treatment after somatic cell nuclear transfer
    Jianguo Zhao
    National Swine Resource and Research Center, Division of Animal Sciences, University of Missouri, Columbia, Missouri 65211, USA
    Biol Reprod 81:525-30. 2009
    ..05). In conclusion, the extremely low cloning efficiency in the NIH miniature pig may be caused by its inbred genetic background and can be improved by alteration of genomic histone acetylation patterns...
  38. pmc Identification and characterization of the porcine (Sus scrofa) survival motor neuron (SMN1) gene: an animal model for therapeutic studies
    Monique A Lorson
    University of Missouri, Department of Veterinary Pathobiology, Life Sciences Center, Columbia, Missouri 65211 7310, USA
    Dev Dyn 237:2268-78. 2008
    ..These studies provide the first characterization of the porcine SMN1 gene and SMN protein and suggest that a transgenic swine SMA model is feasible...
  39. ncbi request reprint Production of cloned pigs by using somatic cells as donors
    Liangxue Lai
    Department of Animal Sciences, University of Missouri Columbia, Missouri 65211 5300, USA
    Cloning Stem Cells 5:233-41. 2003
  40. ncbi request reprint Expression and proteasomal degradation of the major vault protein (MVP) in mammalian oocytes and zygotes
    Peter Sutovsky
    Department of Animal Science, University of Missouri, Columbia, MO 65203, USA
    Reproduction 129:269-82. 2005
    ..Similar to carcinoma cells, MVP could fulfill a cell-protecting function during early embryonic development...
  41. ncbi request reprint The significance of mitochondria for embryo development in cloned farm animals
    Heide Schatten
    Department of Veterinary Pathobiology, School of Veterinary Medicine, University of Missouri Columbia, 1600 E Rollins Street, Columbia, MO 65211, USA
    Mitochondrion 5:303-21. 2005
    ..We review the role of mitochondria in cloned farm animals with emphasis on nucleo-cytoplasmic interactions and consequences for embryo development...
  42. doi request reprint Heat shock of porcine zygotes immediately after oocyte activation increases viability
    S Clay Isom
    Division of Animal Sciences, University of Missouri Columbia, Columbia, Missouri 65211, USA
    Mol Reprod Dev 76:548-54. 2009
    ..A thorough characterization of this phenomenon may yield findings that can serve to increase the efficiency with which PA, IVF, and SCNT embryos are produced in vitro...
  43. pmc Production of piglets after cryopreservation of embryos using a centrifugation-based method for delipation without micromanipulation
    Rongfeng Li
    Division of Animal Science, University of Missouri Columbia, Columbia, Missouri, USA
    Biol Reprod 80:563-71. 2009
    ..Such high-throughput processing overcomes the lack of high efficiency (i.e., the system can be overloaded with embryos for transfer to surrogates)...
  44. pmc Porcine skin-derived stem cells can serve as donor cells for nuclear transfer
    Yanhong Hao
    University of Missouri, Columbia, 65211, USA
    Cloning Stem Cells 11:101-10. 2009
    ..Utilization of porcine SSC may prove to be an excellent cell type for genetic modification followed by nuclear transfer for the production of transgenic pigs...
  45. ncbi request reprint Heat stress-induced apoptosis in porcine in vitro fertilized and parthenogenetic preimplantation-stage embryos
    S Clay Isom
    Division of Animal Sciences, University of Missouri Columbia, Columbia, Missouri 65211, USA
    Mol Reprod Dev 74:574-81. 2007
    ..Parthenogenetic embryos, though, appear to be surprisingly tolerant of the elevated temperatures. The differences between IVF and PA embryos in their response to heat stress warrants further investigation...
  46. pmc Concentration and composition of free amino acids and osmolalities of porcine oviductal and uterine fluid and their effects on development of porcine IVF embryos
    Rongfeng Li
    Division of Animal Science, University of Missouri Columbia, Columbia, Missouri 65211, USA
    Mol Reprod Dev 74:1228-35. 2007
    ..When the embryos were cultured in PZM3-SAA at 330 mOsM for the first 72 hr and then transferred to 250 mOsM (33.3 +/- 3.4%), the blastocyst rate was higher than original PZM3 (21.2 +/- 2.2%) (288 mOsM)...
  47. doi request reprint Analysis of heterogeneous mitochondria distribution in somatic cell nuclear transfer porcine embryos
    Zhisheng Zhong
    Department of Veterinary Pathobiology, University of Missouri Columbia, Columbia, MO 65211, USA
    Microsc Microanal 14:418-32. 2008
    ..2% of embryos, which may negatively influence development; and (2) complex mitochondrial morphologies are observed in IVF and SCNT embryos, which may influence mitochondrial translocation and affect development...
  48. pmc Histone deacetylase inhibitors improve in vitro and in vivo developmental competence of somatic cell nuclear transfer porcine embryos
    Jianguo Zhao
    National Swine Resource and Research Center, University of Missouri, Columbia, Missouri 65211, USA
    Cell Reprogram 12:75-83. 2010
    ..7% for Landrace EFCs. Moreover, treatment of SCNT embryos with Scriptaid improved the histone acetylation on Histone H4 at lysine 8 (AcH4K8) in a pattern similar to that of the in vitro fertilized (IVF) embryos...
  49. ncbi request reprint Ubiquitin C-terminal hydrolase-activity is involved in sperm acrosomal function and anti-polyspermy defense during porcine fertilization
    Young Joo Yi
    Department of Anatomy, University of Missouri Columbia, Missouri 65211, USA
    Biol Reprod 77:780-93. 2007
    ..We conclude that sperm-acrosomal UCHs are involved in sperm-ZP interactions and antipolyspermy defense. Modulation of UCH activity could facilitate the management of polyspermy during IVF and provide insights into male infertility...
  50. ncbi request reprint Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning
    Liangxue Lai
    Department of Animal Science, University of Missouri, Columbia, MO 65211, USA
    Science 295:1089-92. 2002
    ..These pigs were produced by nuclear transfer technology; clonal fetal fibroblast cell lines were used as nuclear donors for embryos reconstructed with enucleated pig oocytes...
  51. ncbi request reprint A method for producing cloned pigs by using somatic cells as donors
    Liangxue Lai
    Animal Sciences Unit, Animal Sciences Research Center, University of Missouri Columbia, USA
    Methods Mol Biol 254:149-64. 2004
  52. ncbi request reprint Apoptosis and in vitro development of preimplantation porcine embryos derived in vitro or by nuclear transfer
    Yanhong Hao
    Department of Animal Sciences, University of Missouri Columbia, Columbia, Missouri 65211, USA
    Biol Reprod 69:501-7. 2003
    ..The developmental rates of NT embryos might be improved by identifying specific apoptotic pathways and then intervening in these pathways to improve development...
  53. ncbi request reprint Production of a transgenic piglet by a sperm injection technique in which no chemical or physical treatments were used for oocytes or sperm
    Hwan Yul Yong
    Division of Animal Science, University of Missouri Columbia, Columbia, Missouri 65211, USA
    Mol Reprod Dev 73:595-9. 2006
    ..These results show the production of the first viable transgenic piglet by in vitro maturation and our new sperm injection method...
  54. ncbi request reprint Effect of elevated Ca(2+) concentration in fusion/activation medium on the fusion and development of porcine fetal fibroblast nuclear transfer embryos
    Hee Tae Cheong
    Department of Animal Sciences, University of Missouri Columbia, Columbia, Missouri 65211, USA
    Mol Reprod Dev 61:488-92. 2002
    ..These results demonstrate that increasing the Ca(2+) concentration in the fusion/activation medium can enhance the fusion and blastocyst formation rates of fetal fibroblast NT porcine embryos without an additional activation treatment...
  55. ncbi request reprint In vitro development of preimplantation porcine nuclear transfer embryos cultured in different media and gas atmospheres
    Gi Sun Im
    Department of Animal Sciences, University of Missouri Columbia, Columbia, MO 65211, USA
    Theriogenology 61:1125-35. 2004
    ..Furthermore, regardless of atmosphere, PZM-3 supported a higher development rate of porcine nuclear transfer embryos than NCSU-23...
  56. ncbi request reprint Apoptosis in parthenogenetic preimplantation porcine embryos
    Yanhong Hao
    Department of Animal Sciences, University of Missouri Columbia, Columbia, Missouri 65211, USA
    Biol Reprod 70:1644-9. 2004
    ..5%) PA blastocysts. The ploidy may contribute to a high level of apoptosis. These results may help to explain the mechanism of parthenogenetic developmental failure and may lead to methods that will improve parthenogenetic development...
  57. ncbi request reprint Cleavage stage porcine embryos may have differing developmental requirements for karyopherins alpha2 and alpha3
    Ryan A Cabot
    Department of Animal Sciences, University of Missouri, 162 Animal Science Research Center, Columbia, Missouri 65211, USA
    Mol Reprod Dev 64:292-301. 2003
    ..This is the first report indicating that mammalian embryos may have differential developmental requirements for specific nuclear trafficking pathways...
  58. ncbi request reprint Progress in producing knockout models for xenotransplantation by nuclear transfer
    Liangxue Lai
    Department of Animal Sciences, University of Missouri Columbia, Columbia, MO 65211, USA
    Ann Med 34:501-6. 2002
    ..Since swine are widely used as models of human disease, the ability to make specific genetic modifications to swine as described here is predicted to have a large impact not only on production agriculture but also on biomedicine...
  59. ncbi request reprint Effects of culture medium, serum type, and various concentrations of follicle-stimulating hormone on porcine preantral follicular development and antrum formation in vitro
    Jiude Mao
    Department of Animal Sciences, University of Missouri Columbia, 65211, USA
    Biol Reprod 67:1197-203. 2002
    ..These results suggest that a longer term culture of preantral follicles (>4 days) may be needed to produce oocytes capable of undergoing meiosis in vitro...
  60. ncbi request reprint CRM1-mediated nuclear export is present during porcine embryogenesis, but is not required for early cleavage
    Ryan A Cabot
    Department of Animal Sciences, University of Missouri, 162 Animal Science Research Center, Columbia, Missouri 65211, USA
    Biol Reprod 67:814-9. 2002
    ..This demonstrates that although CRM1 is present in a functional form throughout mammalian embryo development, its function is not required for early cleavage...
  61. ncbi request reprint Acid peptidase activity released from in vitro produced porcine embryos: a candidate marker to predict developmental competence
    Bhanu Prakash V L Telugu
    Division of Animal Sciences, University of Missouri, Columbia, Missouri 65211, USA
    Mol Reprod Dev 76:417-28. 2009
    ..Therefore, the APA of embryos might serve as an additional marker for evaluation of embryos...
  62. ncbi request reprint Developmental expression of 2489 gene clusters during pig embryogenesis: an expressed sequence tag project
    Kristin Whitworth
    Department of Animal Science, University of Missouri Columbia, Columbia, MO 65211, USA
    Biol Reprod 71:1230-43. 2004
    ..Because many of these ESTs appear to not be in the public databases, their addition will be useful for transcriptional profiling experiments conducted on early pig embryos...
  63. ncbi request reprint Osteopontin improves in vitro development of porcine embryos and decreases apoptosis
    Yanhong Hao
    Division of Animal Sciences, University of Missouri Columbia, Columbia, Missouri, USA
    Mol Reprod Dev 75:291-8. 2008
    ..9 +/- 0.04(a) vs. 13.1 +/- 0.02(b)) and the percent fragmentation (45.2 +/- 0.07(a) vs. 58.8 +/- 0.03(b)). We conclude that SPP1 can improve development in vitro possibly by reducing the rate of apoptosis...
  64. ncbi request reprint Osteopontin reduces polyspermy during in vitro fertilization of porcine oocytes
    Yanhong Hao
    Division of Animal Sciences, University of Missouri Columbia, Missouri 65211, USA
    Biol Reprod 75:726-33. 2006
    ..The current study represents the first report to demonstrate that SPP1 plays an important role in the regulation of pig polyspermic fertilization; it decreases polyspermy and increases fertilization efficiency during IVF...
  65. pmc Development and calcium level changes in pre-implantation porcine nuclear transfer embryos activated with 6-DMAP after fusion
    Gi Sun Im
    Division of Animal Sciences, University of Missouri Columbia, Columbia, Missouri 65211, USA
    Mol Reprod Dev 74:1158-64. 2007
    ..Activation of NT embryos with 6-DMAP after fusion in the presence of 1.0 mM CaCl(2) could support better developmental rate to the blastocyst stage...
  66. ncbi request reprint Phenotyping of transgenic cloned piglets
    D Bart Carter
    Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri 65211, USA
    Cloning Stem Cells 4:131-45. 2002
    ..During the course of this study, five of the 10 piglets were euthanized or died, indicating there is a high mortality rate among cloned piglets, but the remaining five cloned piglets are free from detectable defects...
  67. ncbi request reprint Fragmentation and development of preimplantation porcine embryos derived by parthenogenetic activation and nuclear transfer
    Gi Sun Im
    Department of Animal Sciences, University of Missouri Columbia, Columbia, Missouri 65211, USA
    Mol Reprod Dev 71:159-65. 2005
    ....
  68. ncbi request reprint Cyclin B1 levels in the porcine 4-cell stage embryo
    Aaron J Bonk
    Department of Animal Sciences, University of Missouri Columbia, 65211, USA
    Zygote 10:79-84. 2002
    ..In addition, cyclin B1 protein levels remained constant in the absence of embryonic genome activation at the 4-cell stage...
  69. ncbi request reprint Developmental competence of porcine parthenogenetic embryos relative to embryonic chromosomal abnormalities
    Yan Hong Hao
    Division of Animal Sciences, University of Missouri Columbia, Columbia, Missouri 65211, USA
    Mol Reprod Dev 73:77-82. 2006
    ..We suggest that a careful consideration should be given when using the blastocysts with smaller size, and establishing the optimum culture condition for PA embryos development in vitro...
  70. doi request reprint Transcriptional profiling of day 12 porcine embryonic disc and trophectoderm samples using ultra-deep sequencing technologies
    S Clay Isom
    Division of Animal Science, University of Missouri, Columbia, Missouri, USA
    Mol Reprod Dev 77:812-9. 2010
    ..We also demonstrate the utility of pre-genome genomics resources-such as EST repositories-in the analysis and application of next-generation sequencing data in the absence of an appropriately annotated reference genome...
  71. ncbi request reprint Centrosome abnormalities during porcine oocyte aging
    Yi Liang Miao
    Department of Veterinary Pathobiology, University of Missouri, 1600 E Rollins Street, Columbia, MO 65211, USA
    Environ Mol Mutagen 50:666-71. 2009
    ....
  72. ncbi request reprint Large-scale generation and analysis of expressed sequence tags from porcine ovary
    Honglin Jiang
    Department of Animal Science, University of Missouri, Columbia, Missouri 65211, USA
    Biol Reprod 71:1991-2002. 2004
    ..This porcine ovary unigene set will be useful for identifying factors and mechanisms controlling ovarian follicular development in a variety of species...
  73. ncbi request reprint Premature estrogen exposure alters endometrial gene expression to disrupt pregnancy in the pig
    Jason W Ross
    Department of Animal Science, Oklahoma State University, Stillwater, OK 74078, USA
    Endocrinology 148:4761-73. 2007
    ....
  74. ncbi request reprint Highly efficient and reliable chemically assisted enucleation method for handmade cloning in cattle
    Gabor Vajta
    Population Genetics and Embryology, Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, DK 8830 Tjele, Denmark
    Reprod Fertil Dev 17:791-7. 2005
    ..Moreover, expensive equipment (inverted fluorescence microscope) and a potentially harmful step (staining and ultraviolet illumination) can be eliminated from the HMC procedure without compromising the high in vitro efficiency...
  75. ncbi request reprint Capacitative calcium entry mechanism in porcine oocytes
    Zoltan Machaty
    Alexion Pharmaceuticals, Inc, Cheshire, Connecticut 06410, USA
    Biol Reprod 66:667-74. 2002
    ....
  76. ncbi request reprint Cloning pigs as organ donors for humans
    Liangxue Lai
    IEEE Eng Med Biol Mag 23:37-42. 2004
  77. ncbi request reprint Na(+)/Ca(2+) exchanger in porcine oocytes
    Zoltan Machaty
    Alexion Pharmaceuticals, Inc, Cheshire, Connecticut 06410, USA
    Biol Reprod 67:1133-9. 2002
    ..Reverse transcription-polymerase chain reaction revealed that porcine oocytes contain a transcript that shows 98.1% homology to the NACA3 isoform of the porcine Na(+)/Ca(2+) exchanger...
  78. ncbi request reprint PAWP, a sperm-specific WW domain-binding protein, promotes meiotic resumption and pronuclear development during fertilization
    Alexander T H Wu
    Department of Anatomy and Cell Biology, Queen s University, Kingston, Ontario K7L 3N6, Canada
    J Biol Chem 282:12164-75. 2007
    ....
  79. pmc Isolation of progenitor cells from GFP-transgenic pigs and transplantation to the retina of allorecipients
    Henry Klassen
    Department of Ophthalmology, School of Medicine, University of California, Irvine, Orange, California 92868 4380, USA
    Cloning Stem Cells 10:391-402. 2008
    ..These findings demonstrate the feasibility of allogeneic progenitor transplantation in a large mammal and the utility of the pig in ocular regeneration studies...
  80. ncbi request reprint Activation of ribosomal RNA genes in porcine embryos produced in vitro or by somatic cell nuclear transfer
    Bolette Bjerregaard
    Department of Animal and Veterinary Basic Sciences, The Royal Veterinary and Agricultural University, Frederiksberg C, Denmark
    Mol Reprod Dev 74:35-41. 2007
    ..In conclusion, porcine embryos produced in vitro had an asynchronous pattern of rRNA transcription initiation when compared to SCNT and in vivo developed porcine embryos...
  81. ncbi request reprint Regulation of prohibitin expression during follicular development and atresia in the mammalian ovary
    Winston E Thompson
    Department of Obstetrics and Gynecology and Cooperative Reproductive Science Research Center, Morehouse School of Medicine, Atlanta, GA 30310, USA
    Biol Reprod 71:282-90. 2004
    ..It is likely that prohibitin may play an important role in determining the fate of these cells and eventual follicular destiny...
  82. pmc Production of alpha-1,3-galactosyltransferase null pigs by means of nuclear transfer with fibroblasts bearing loss of heterozygosity mutations
    Donna Kolber-Simonds
    Immerge BioTherapeutics, Inc, 300 Technology Square, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 101:7335-40. 2004
    ..In fact, congenital abnormalities observed in the heterozygous NT animals did not reappear in the serially produced null animals...
  83. ncbi request reprint Telomere lengths in cloned transgenic pigs
    Le Jiang
    Department of Animal Science and Center for Regenerative Biology, University of Connecticut, Storrs, Connecticut 06269, USA
    Biol Reprod 70:1589-93. 2004
    ..Two cloned pigs that died shortly after birth had skin telomere lengths equivalent to those of late-stage fetuses...
  84. pmc Production of CFTR-null and CFTR-DeltaF508 heterozygous pigs by adeno-associated virus-mediated gene targeting and somatic cell nuclear transfer
    Christopher S Rogers
    Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, Iowa, USA
    J Clin Invest 118:1571-7. 2008
    ..In addition, because gene-modified mice often fail to replicate human diseases, this approach could be used to generate models of other human genetic diseases in species other than mice...
  85. pmc Expression of X-linked genes in deceased neonates and surviving cloned female piglets
    Le Jiang
    Department of Animal Science and Center for Regenerative Biology, University of Connecticut, Storrs, Connecticut, USA
    Mol Reprod Dev 75:265-73. 2008
    ..These results suggest a correlation between the viability of the clones and the normality of their gene expression and provide a possible explanation for the death of a large portion of cloned animals around birth...
  86. pmc The porcine lung as a potential model for cystic fibrosis
    Christopher S Rogers
    Department of Internal Medicine, Roy J Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
    Am J Physiol Lung Cell Mol Physiol 295:L240-63. 2008
    ..Thus pigs with a targeted CFTR gene might provide a good model for CF. Here, we review aspects of porcine airways and lung that are relevant to CF...
  87. ncbi request reprint Luteinization of porcine preovulatory follicles leads to systematic changes in follicular gene expression
    Cansu Agca
    Department of Animal Science, University of Missouri, Columbia, Missouri 65211, USA
    Reproduction 132:133-45. 2006
    ..Cells within preovulatory luteinized follicles had a gene-expression profile of nonproliferative and migratory cells with angiogenic properties. Approximately, 40% of the discovered genes had unknown function...
  88. ncbi request reprint Evaluation of the acute phase response in cloned pigs following a lipopolysaccharide challenge
    Jeffery A Carroll
    Livestock Issues Research Unit, USDA, ARS, Lubbock, TX 79403, USA
    Domest Anim Endocrinol 29:564-72. 2005
    ....
  89. ncbi request reprint Expression levels of growth-regulating imprinted genes in cloned piglets
    Le Jiang
    Department of Animal Science and Center for Regenerative Biology, University of Connecticut, Storrs, Connecticut, USA
    Cloning Stem Cells 9:97-106. 2007
    ..Interestingly, larger variances of gene expression were found in identical clones at 1 month old when compared to the control animals, indicating the variability of the nuclear reprogramming process...
  90. ncbi request reprint Development and apoptosis of pre-implantation porcine nuclear transfer embryos activated with different combination of chemicals
    Gi Sun Im
    Division of Animal Sciences, University of Missouri Columbia, 65211, USA
    Mol Reprod Dev 73:1094-101. 2006
    ..However, the chemical activation also could increase the frequency of apoptosis and induced apoptosis earlier in porcine NT embryos...

Research Grants13

  1. Reproduction of Transgenic Pigs by Nuclear Transplantation
    RANDALL PRATHER; Fiscal Year: 2009
    ..The third aim is to determine the methylation profile and transcriptional profile of two different types of stem cells as compared to fetal-derived fibroblast-like cells. ..
  2. PRODUCTION OF TRANSGENIC PIGS BY NUCLEAR TRANSPLANTATION
    RANDALL PRATHER; Fiscal Year: 2006
    ..Here, the investigators will evaluate a few specific parameters of what makes nuclear transfer successful or unsuccessful. ..
  3. PRODUCTION OF TRANSGENIC PIGS BY NUCLEAR TRANSPLANTATION
    RANDALL PRATHER; Fiscal Year: 2005
    ..Here, the investigators will evaluate a few specific parameters of what makes nuclear transfer successful or unsuccessful. ..
  4. PRODUCTION OF TRANSGENIC PIGS BY NUCLEAR TRANSPLANTATION
    RANDALL PRATHER; Fiscal Year: 2004
    ..Here, the investigators will evaluate a few specific parameters of what makes nuclear transfer successful or unsuccessful. ..
  5. PRODUCTION OF TRANSGENIC PIGS BY NUCLEAR TRANSPLANTATION
    RANDALL PRATHER; Fiscal Year: 2003
    ..Here, the investigators will evaluate a few specific parameters of what makes nuclear transfer successful or unsuccessful. ..
  6. PRODUCTION OF TRANSGENICS BY NUCLEAR TRANSPLANTATION
    RANDALL PRATHER; Fiscal Year: 1999
    ..Development of this technology in the pig will facilitate genetic knock-outs and knock-ins. Development of the nuclear transfer technology may have wide utility. ..
  7. Reproduction of Transgenic Pigs by Nuclear Transplantation
    Randall S Prather; Fiscal Year: 2010
    ..The third aim is to determine the methylation profile and transcriptional profile of two different types of stem cells as compared to fetal-derived fibroblast-like cells. ..
  8. PRODUCTION OF TRANSGENIC PIGS BY NUCLEAR TRANSPLANTATION
    RANDALL PRATHER; Fiscal Year: 2002
    ..Here, the investigators will evaluate a few specific parameters of what makes nuclear transfer successful or unsuccessful. ..
  9. PRODUCTION OF TRANSGENICS BY NUCLEAR TRANSPLANTATION
    RANDALL PRATHER; Fiscal Year: 2001
    ..Development of this technology in the pig will facilitate genetic knock-outs and knock-ins. Development of the nuclear transfer technology may have wide utility. ..
  10. PRODUCTION OF TRANSGENICS BY NUCLEAR TRANSPLANTATION
    RANDALL PRATHER; Fiscal Year: 2000
    ..Development of this technology in the pig will facilitate genetic knock-outs and knock-ins. Development of the nuclear transfer technology may have wide utility. ..
  11. Reproduction of Transgenic Pigs by Nuclear Transplantation
    RANDALL PRATHER; Fiscal Year: 2007
    ..The third aim is to determine the methylation profile and transcriptional profile of two different types of stem cells as compared to fetal-derived fibroblast-like cells. ..