Affiliation: University of California
Hill A, Niles B, Cuyegkeng A, Powers T. Redesigning TOR Kinase to Explore the Structural Basis for TORC1 and TORC2 Assembly. Biomolecules. 2018;8: pubmed publisher
..Our findings are in excellent agreement with recent ultrastructural studies of TORC1 and TORC2, where the MAS domain is involved in quaternary interactions important for complex formation and/or stability. ..
Stauffer B, Powers T. Target of rapamycin signaling mediates vacuolar fission caused by endoplasmic reticulum stress in Saccharomyces cerevisiae. Mol Biol Cell. 2015;26:4618-30 pubmed publisher
..Together these results reveal a new role for TORC1 in the regulation of vacuolar behavior. ..
Stauffer B, Powers T. Target of rapamycin signaling mediates vacuolar fragmentation. Curr Genet. 2017;63:35-42 pubmed publisher
..Together these advances both extend our understanding of the regulation of vacuolar fragmentation in yeast as well as underscore the role of analogous events in mammalian cells. ..
Niles B, Joslin A, Fresques T, Powers T. TOR complex 2-Ypk1 signaling maintains sphingolipid homeostasis by sensing and regulating ROS accumulation. Cell Rep. 2014;6:541-52 pubmed publisher
..Thus, ROS sensing and signaling by TORC2/Ypk1 play a central physiological role in sphingolipid biosynthesis and in the maintenance of cell growth and viability. ..
Vlahakis A, Graef M, Nunnari J, Powers T. TOR complex 2-Ypk1 signaling is an essential positive regulator of the general amino acid control response and autophagy. Proc Natl Acad Sci U S A. 2014;111:10586-91 pubmed publisher
..Our work reveals that the TORC2 pathway regulates autophagy in an opposing manner to TORC1 to provide a tunable response to cellular metabolic status. ..
Niles B, Powers T. TOR complex 2-Ypk1 signaling regulates actin polarization via reactive oxygen species. Mol Biol Cell. 2014;25:3962-72 pubmed publisher
Fresques T, Niles B, Aronova S, Mogri H, Rakhshandehroo T, Powers T. Regulation of ceramide synthase by casein kinase 2-dependent phosphorylation in Saccharomyces cerevisiae. J Biol Chem. 2015;290:1395-403 pubmed publisher
Vlahakis A, Lopez Muniozguren N, Powers T. Calcium channel regulator Mid1 links TORC2-mediated changes in mitochondrial respiration to autophagy. J Cell Biol. 2016;215:779-788 pubmed
..These findings describe a novel pathway involving TORC2, mitochondrial oxidative stress, and calcium homeostasis for autophagy regulation. ..