Anthony R Poteete

Summary

Affiliation: University of Massachusetts Medical School
Country: USA

Publications

  1. ncbi request reprint PCR-mediated gene replacement in Escherichia coli
    K C Murphy
    Department of Molecular Genetics and Microbiology University of Massachusetts Medical School, Worcester, MA 01655, USA
    Gene 246:321-30. 2000
  2. pmc Recombination phenotypes of Escherichia coli greA mutants
    Anthony R Poteete
    Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, MA, USA
    BMC Mol Biol 12:12. 2011
  3. pmc Expansion of a chromosomal repeat in Escherichia coli: roles of replication, repair, and recombination functions
    Anthony R Poteete
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA, USA
    BMC Mol Biol 10:14. 2009
  4. doi request reprint Involvement of DNA replication in phage lambda Red-mediated homologous recombination
    Anthony R Poteete
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA, USA
    Mol Microbiol 68:66-74. 2008
  5. ncbi request reprint Gentamicin and other cassettes for chromosomal gene replacement in Escherichia coli
    Anthony R Poteete
    University of Massachusetts Medical School, Department of Molecular Genetics and Microbiology, Worcester, MA 01655, USA
    Biotechniques 41:261-2, 264. 2006
  6. pmc Chromosomal duplications and cointegrates generated by the bacteriophage lamdba Red system in Escherichia coli K-12
    Anthony R Poteete
    Dept, of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    BMC Mol Biol 5:22. 2004
  7. pmc Modulation of DNA repair and recombination by the bacteriophage lambda Orf function in Escherichia coli K-12
    Anthony R Poteete
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue, Worcester, MA 01655, USA
    J Bacteriol 186:2699-707. 2004
  8. pmc Recombination-promoting activity of the bacteriophage lambda Rap protein in Escherichia coli K-12
    Anthony R Poteete
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Bacteriol 184:4626-9. 2002
  9. pmc Phage lambda red-mediated adaptive mutation
    Anthony R Poteete
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Bacteriol 184:3753-5. 2002
  10. ncbi request reprint What makes the bacteriophage lambda Red system useful for genetic engineering: molecular mechanism and biological function
    A R Poteete
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    FEMS Microbiol Lett 201:9-14. 2001

Collaborators

Detail Information

Publications15

  1. ncbi request reprint PCR-mediated gene replacement in Escherichia coli
    K C Murphy
    Department of Molecular Genetics and Microbiology University of Massachusetts Medical School, Worcester, MA 01655, USA
    Gene 246:321-30. 2000
    ..coli. Three of these replacements were performed without prior cloning of the genes...
  2. pmc Recombination phenotypes of Escherichia coli greA mutants
    Anthony R Poteete
    Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, MA, USA
    BMC Mol Biol 12:12. 2011
    ..Replication fork collapse is known to generate dsDNA breaks, which can be recombinogenic. It follows that GreA malfunction could have consequences affecting homologous recombination...
  3. pmc Expansion of a chromosomal repeat in Escherichia coli: roles of replication, repair, and recombination functions
    Anthony R Poteete
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA, USA
    BMC Mol Biol 10:14. 2009
    ..To explore the mechanism of expansion, a 7 kbp duplication in the chromosome containing a leaky mutant version of the lac operon was constructed, and its expansion into an amplified array was studied...
  4. doi request reprint Involvement of DNA replication in phage lambda Red-mediated homologous recombination
    Anthony R Poteete
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA, USA
    Mol Microbiol 68:66-74. 2008
    ..A third mechanism, replisome invasion, involving replication directly in the recombination mechanism, is invoked as an alternative...
  5. ncbi request reprint Gentamicin and other cassettes for chromosomal gene replacement in Escherichia coli
    Anthony R Poteete
    University of Massachusetts Medical School, Department of Molecular Genetics and Microbiology, Worcester, MA 01655, USA
    Biotechniques 41:261-2, 264. 2006
  6. pmc Chromosomal duplications and cointegrates generated by the bacteriophage lamdba Red system in Escherichia coli K-12
    Anthony R Poteete
    Dept, of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    BMC Mol Biol 5:22. 2004
    ..This study characterizes the genetic requirements and outcomes of a variety of additional Red-promoted homologous recombination events producing Lac+ recombinants...
  7. pmc Modulation of DNA repair and recombination by the bacteriophage lambda Orf function in Escherichia coli K-12
    Anthony R Poteete
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue, Worcester, MA 01655, USA
    J Bacteriol 186:2699-707. 2004
    ..In a fully wild-type background with respect to known recombination and repair functions, orf partially suppressed the UV sensitivity of ruvAB and ruvC mutants...
  8. pmc Recombination-promoting activity of the bacteriophage lambda Rap protein in Escherichia coli K-12
    Anthony R Poteete
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Bacteriol 184:4626-9. 2002
    ..The results of these experiments indicated that the lambda rap gene could functionally substitute for the E. coli ruvC gene in Red-mediated recombination...
  9. pmc Phage lambda red-mediated adaptive mutation
    Anthony R Poteete
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Bacteriol 184:3753-5. 2002
    ..Like RecBCD-dependent adaptive mutation, Red-mediated adaptive mutation is dependent upon recA and ruvABC functions...
  10. ncbi request reprint What makes the bacteriophage lambda Red system useful for genetic engineering: molecular mechanism and biological function
    A R Poteete
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    FEMS Microbiol Lett 201:9-14. 2001
    ..The role of high-frequency double-strand break repair/recombination in the life cycle of the lambdoid phages is discussed...
  11. pmc Genetic requirements of phage lambda red-mediated gene replacement in Escherichia coli K-12
    A R Poteete
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Bacteriol 182:2336-40. 2000
    ..Expression of a gene(s) from the nin region of phage lambda partially complemented both the viability and recombination defects of the recF, recO, and recR mutants and the recombination defect of ruvC but not of ruvAB or recQ mutants...
  12. pmc Roles of RuvC and RecG in phage lambda red-mediated recombination
    A R Poteete
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Bacteriol 181:5402-8. 1999
    ..However, in all cases, the frequency of Red-mediated recombination was higher in recG than in recG+. These observations favor models in which RecG tends to push invading 3'-ended strands back out of recombination intermediates...
  13. pmc Alteration of T4 lysozyme structure by second-site reversion of deleterious mutations
    A R Poteete
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical Center, Worcester 01655, USA
    Protein Sci 6:2418-25. 1997
    ..Some variants of proteins bearing primary substitutions at the positions of Asp 20 and Ala 98 are inferred to have significantly altered structures...
  14. pmc Amplification of lac cannot account for adaptive mutation to Lac+ in Escherichia coli
    Jeffrey D Stumpf
    Department of Biology, Indiana University, 1001 East Third Street, Bloomington, IN 47405, USA
    J Bacteriol 189:2291-9. 2007
    ..Our results support the hypothesis that most Lac+ mutants that appear during lactose selection are true revertants that arise in a single step from Lac- cells, not from a population of growing or amplifying precursor cells...