Research Topics
Species | A R PoteeteSummaryAffiliation: University of Massachusetts Medical Center Country: USA Publications
| Collaborators |
Detail Information
Publications
Alteration of T4 lysozyme structure by second-site reversion of deleterious mutationsA R Poteete
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical Center, Worcester 01655, USA
Protein Sci 6:2418-25. 1997..Some variants of proteins bearing primary substitutions at the positions of Asp 20 and Ala 98 are inferred to have significantly altered structures...
Roles of RuvC and RecG in phage lambda red-mediated recombinationA R Poteete
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Bacteriol 181:5402-8. 1999..However, in all cases, the frequency of Red-mediated recombination was higher in recG than in recG+. These observations favor models in which RecG tends to push invading 3'-ended strands back out of recombination intermediates...
Genetic requirements of phage lambda red-mediated gene replacement in Escherichia coli K-12A R Poteete
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
J Bacteriol 182:2336-40. 2000..Expression of a gene(s) from the nin region of phage lambda partially complemented both the viability and recombination defects of the recF, recO, and recR mutants and the recombination defect of ruvC but not of ruvAB or recQ mutants...
PCR-mediated gene replacement in Escherichia coliK C Murphy
Department of Molecular Genetics and Microbiology University of Massachusetts Medical School, Worcester, MA 01655, USA
Gene 246:321-30. 2000..coli. Three of these replacements were performed without prior cloning of the genes...
What makes the bacteriophage lambda Red system useful for genetic engineering: molecular mechanism and biological functionA R Poteete
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
FEMS Microbiol Lett 201:9-14. 2001..The role of high-frequency double-strand break repair/recombination in the life cycle of the lambdoid phages is discussed...
Superinfection exclusion (sieB) genes of bacteriophages P22 and lambdaK Ranade
Department of Molecular Genetics and Microbiology, University of Massachusetts, Worcester 01655
J Bacteriol 175:4712-8. 1993..The sieB promoter thus identified was inactivated by a 2-base substitution in its -10 hexamer. The sieB gene of coliphage lambda was also identified. The promoter for lambda sieB was identified by homology to that of P22 sieB...
