Matthew Porteus

Summary

Affiliation: University of Texas Southwestern Medical Center
Country: USA

Publications

  1. pmc Efficient gene targeting mediated by adeno-associated virus and DNA double-strand breaks
    Matthew H Porteus
    California Institute of Technology, Pasadena, CA 91125, USA
    Mol Cell Biol 23:3558-65. 2003
  2. ncbi request reprint Using homologous recombination to manipulate the genome of human somatic cells
    Matthew Porteus
    Departments of Pediatrics and Biochemistry, UT Southwestern Medical Center Dallas, TX 75214, USA
    Biotechnol Genet Eng Rev 24:195-212. 2007
  3. doi request reprint Comparison of zinc finger nucleases for use in gene targeting in mammalian cells
    Shondra M Pruett-Miller
    Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    Mol Ther 16:707-17. 2008
  4. ncbi request reprint Chimeric nucleases stimulate gene targeting in human cells
    Matthew H Porteus
    Department of Biology, California Institute of Technology, Pasadena CA 91125, USA
    Science 300:763. 2003
  5. ncbi request reprint Gene targeting using zinc finger nucleases
    Matthew H Porteus
    Department of Pediatrics, University of Texas Southwestern Medical Center, USA
    Nat Biotechnol 23:967-73. 2005
  6. ncbi request reprint Mammalian gene targeting with designed zinc finger nucleases
    Matthew H Porteus
    Department of Pediatrics, University of Texas Southwestern Medical School, Dallas, 75390 9063, USA
    Mol Ther 13:438-46. 2006
  7. pmc A look to future directions in gene therapy research for monogenic diseases
    Matthew H Porteus
    PLoS Genet 2:e133. 2006
  8. doi request reprint Design and testing of zinc finger nucleases for use in mammalian cells
    Matthew Porteus
    Department of Pediatrics and Biochemistry, UT Southwestern Medical Center, Dallas, TX, USA
    Methods Mol Biol 435:47-61. 2008
  9. pmc Gene correction by homologous recombination with zinc finger nucleases in primary cells from a mouse model of a generic recessive genetic disease
    Jon P Connelly
    Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    Mol Ther 18:1103-10. 2010
  10. doi request reprint Gene therapy for primary immunodeficiencies
    Eric Kildebeck
    Department of Pediatrics, Stanford University School of Medicine, California 94305, USA
    Curr Opin Pediatr 24:731-8. 2012

Collaborators

  • F D Urnov
  • Dana Carroll
  • Patrick Ryan Potts
  • Christina Tenenhaus Dann
  • Matthew D Weitzman
  • Toni Cathomen
  • Hongtao Yu
  • Jeffry D Sander
  • Shondra M Pruett-Miller
  • Jon P Connelly
  • Morgan L Maeder
  • J Keith Joung
  • David A Wright
  • Eric Kildebeck
  • Fengli Fu
  • Stacey Thibodeau-Beganny
  • Magdalena Eichtinger
  • Ronnie J Winfrey
  • Daniel F Voytas
  • Drena Dobbs
  • Sundar Durai
  • Josh Checketts
  • Shondra Pruett-Miller
  • Jenny C Barker
  • David W Reading
  • Shaina N Porter
  • Dennis C Sgroi
  • Anna Osiak
  • Jeffrey A Townsend
  • Reshma M Anthony
  • Erica Unger-Wallace
  • Felix Müller-Lerch
  • Paul B McCray
  • A John Iafrate
  • Carl Göbel
  • Jonathan E Foley
  • Justin P Dassie
  • Tao Jiang
  • Joseph Pearlberg
  • Andrew S Hirsh
  • Mala Mani
  • Joy Wu
  • Srinivasan Chandrasegaran
  • Karthikeyan Kandavelou

Detail Information

Publications18

  1. pmc Efficient gene targeting mediated by adeno-associated virus and DNA double-strand breaks
    Matthew H Porteus
    California Institute of Technology, Pasadena, CA 91125, USA
    Mol Cell Biol 23:3558-65. 2003
    ..The frequencies of gene targeting that we achieved with relatively low MOIs suggest that combining rAAV vectors with DSBs is a promising strategy to broaden the application of gene targeting...
  2. ncbi request reprint Using homologous recombination to manipulate the genome of human somatic cells
    Matthew Porteus
    Departments of Pediatrics and Biochemistry, UT Southwestern Medical Center Dallas, TX 75214, USA
    Biotechnol Genet Eng Rev 24:195-212. 2007
  3. doi request reprint Comparison of zinc finger nucleases for use in gene targeting in mammalian cells
    Shondra M Pruett-Miller
    Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    Mol Ther 16:707-17. 2008
    ..We demonstrate that a pair of three-finger B2H ZFNs is as efficient at stimulating gene targeting as ZFNs with more fingers, and induce similar or lower rates of toxicity...
  4. ncbi request reprint Chimeric nucleases stimulate gene targeting in human cells
    Matthew H Porteus
    Department of Biology, California Institute of Technology, Pasadena CA 91125, USA
    Science 300:763. 2003
  5. ncbi request reprint Gene targeting using zinc finger nucleases
    Matthew H Porteus
    Department of Pediatrics, University of Texas Southwestern Medical Center, USA
    Nat Biotechnol 23:967-73. 2005
    ..Future work will be needed to translate these in vitro findings to in vivo applications and to determine whether zinc finger nucleases create undesired genomic instability...
  6. ncbi request reprint Mammalian gene targeting with designed zinc finger nucleases
    Matthew H Porteus
    Department of Pediatrics, University of Texas Southwestern Medical School, Dallas, 75390 9063, USA
    Mol Ther 13:438-46. 2006
    ....
  7. pmc A look to future directions in gene therapy research for monogenic diseases
    Matthew H Porteus
    PLoS Genet 2:e133. 2006
    ....
  8. doi request reprint Design and testing of zinc finger nucleases for use in mammalian cells
    Matthew Porteus
    Department of Pediatrics and Biochemistry, UT Southwestern Medical Center, Dallas, TX, USA
    Methods Mol Biol 435:47-61. 2008
    ..This chapter describes how to identify potential targets for ZFN cutting, to make ZFNs to cut this target site, and how to test whether the newly designed ZFNs are active in a mammalian cell culture-based system...
  9. pmc Gene correction by homologous recombination with zinc finger nucleases in primary cells from a mouse model of a generic recessive genetic disease
    Jon P Connelly
    Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    Mol Ther 18:1103-10. 2010
    ..In summary, potentially therapeutically relevant frequencies of ZFN-mediated gene targeting can be achieved in a variety of primary cells and these cells can then be transplanted back into a recipient...
  10. doi request reprint Gene therapy for primary immunodeficiencies
    Eric Kildebeck
    Department of Pediatrics, Stanford University School of Medicine, California 94305, USA
    Curr Opin Pediatr 24:731-8. 2012
    ..In this review, we discuss recent advances in gene therapy with an emphasis on strategies to improve safety, including the emergence of gene targeting technologies for the treatment of PIDs...
  11. pmc Human SMC5/6 complex promotes sister chromatid homologous recombination by recruiting the SMC1/3 cohesin complex to double-strand breaks
    Patrick Ryan Potts
    Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, TX 75390 9041, USA
    EMBO J 25:3377-88. 2006
    ..Our results establish a mechanism by which the hSMC5/6 complex promotes DNA repair and suggest a novel strategy to improve the efficiency of gene targeting in mammalian somatic cells...
  12. pmc Attenuation of zinc finger nuclease toxicity by small-molecule regulation of protein levels
    Shondra M Pruett-Miller
    Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America
    PLoS Genet 5:e1000376. 2009
    ..We show that by regulating protein levels, we can maintain high rates of ZFN-mediated gene targeting while reducing ZFN toxicity...
  13. doi request reprint Homologous recombination-based gene therapy for the primary immunodeficiencies
    Matthew Porteus
    Department of Pediatrics, Divisions of Cancer Biology, Hematology Oncology, Human Gene Therapy, Stanford University, Stanford, California, USA
    Ann N Y Acad Sci 1246:131-40. 2011
    ..In this review, I discuss the development of nuclease-stimulated, homologous recombination-based approaches as a novel gene therapy strategy for the primary immunodeficiencies...
  14. doi request reprint Spermatogonial stem cell self-renewal requires OCT4, a factor downregulated during retinoic acid-induced differentiation
    Christina Tenenhaus Dann
    Departments of Pediatrics, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, USA
    Stem Cells 26:2928-37. 2008
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  15. ncbi request reprint Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly
    David A Wright
    Department of Genetics, Development and Cell Biology, Iowa State University, 1035A Roy J Carver Co Lab, Ames, Iowa 50011, USA
    Nat Protoc 1:1637-52. 2006
    ..We also describe a bacterial cell-based reporter assay for rapidly screening the DNA-binding activities of assembled multi-finger arrays. This protocol can be completed in approximately 24-26 d...
  16. pmc Zinc finger nucleases: custom-designed molecular scissors for genome engineering of plant and mammalian cells
    Sundar Durai
    Department of Environmental Health Sciences, The Johns Hopkins University Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, MD 21205 2179, USA
    Nucleic Acids Res 33:5978-90. 2005
    ....
  17. pmc Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification
    Morgan L Maeder
    Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Mol Cell 31:294-301. 2008
    ....
  18. ncbi request reprint Highly efficient endogenous human gene correction using designed zinc-finger nucleases
    Fyodor D Urnov
    Sangamo BioSciences, Inc, Pt Richmond Tech Center 501, Canal Blvd, Suite A100 Richmond, California 94804, USA
    Nature 435:646-51. 2005
    ..We observe comparably high frequencies in human T cells, raising the possibility of strategies based on zinc-finger nucleases for the treatment of disease...

Research Grants6

  1. The Genetics of Gene Targeting in Vertebrate Cells
    Matthew Porteus; Fiscal Year: 2006
    ..It is hoped that such studies will lead to advances in the field of gene therapy and in the treatment of children with monogenic diseases, such as sickle cell disease. ..
  2. Zinc Finger Nucleases to Stimulate Gene Targeting in Hematopoietic Stem Cells
    Matthew Porteus; Fiscal Year: 2007
    ..The goal of these studies is both to understand the biology of gene targeting in these cells and to use that understanding to develop targeting as a therapeutic tool to treat monogenic diseases such as sickle cell disease. ..
  3. Development of gene targeting in C. elegans and D. rerio using zinc finger
    Matthew Porteus; Fiscal Year: 2007
    ..We expect that, if successful, this novel approach would be a practical, flexible, and powerful technique that would find wide application, significantly increasing the power of these systems to illuminate human cancer biology. ..
  4. Using Zinc Finger Nucleases to Stimulate Gene Targeting in HSC
    Matthew Porteus; Fiscal Year: 2009
    ..abstract_text> ..