Research Topics
| Christopher PoradaSummaryAffiliation: University of Nevada Country: USA Publications
Research Grants
| Collaborators
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Detail Information
Publications
Mesenchymal stem cells as therapeutics and vehicles for gene and drug deliveryChristopher D Porada
Department of Animal Biotechnology, University of Nevada, Reno, 89557 0104, USA
Adv Drug Deliv Rev 62:1156-66. 2010....- Development and characterization of a novel CD34 monoclonal antibody that identifies sheep hematopoietic stem/progenitor cellsChristopher D Porada
Department of Animal Biotechnology, School of Veterinary Medicine, University of Nevada, Reno, NV 89557 0104, USA
Exp Hematol 36:1739-49. 2008..The current studies were undertaken to create a monoclonal antibody to sheep CD34 that would enable isolation and study of sheep HSC/progenitors... 
Male germ-line cells are at risk following direct-injection retroviral-mediated gene transfer in uteroChristopher D Porada
Department of Animal Biotechnology, University of Nevada at Reno, 89557 0104, USA
Mol Ther 12:754-62. 2005..In conclusion, our analysis on over 3 x 10(9) sperm cells suggests that the direct-injection approach employed in these studies may result in the inadvertent transduction of very low numbers of male germ cells...- Plasticity of human stem cells in the fetal sheep model of human stem cell transplantationGraca Almeida-Porada
Department of Animal Biotechnology, University of Nevada, Reno, Nevada 89557 0104, USA
Int J Hematol 79:1-6. 2004..In this review, we describe the advantages of the permissive aspects of the developing preimmune fetus in the early gestational age that led us to develop the sheep as a large-animal model of human stem cell plasticity... - Factors determining the risk of inadvertent retroviral transduction of male germ cells after in utero gene transfer in sheepPaul J Park
Department of Animal Biotechnology, School of Veterinary Medicine, University of Nevada, Reno, NV 89557, USA
Hum Gene Ther 20:201-15. 2009.... - The human-sheep chimeras as a model for human stem cell mobilization and evaluation of hematopoietic grafts' potentialGraca Almeida-Porada
Department of Animal Biotechnology University of Nevada, Reno, NV 89557 0104, USA
Exp Hematol 35:1594-600. 2007..To investigate whether the sheep xenograft model of human hematopoiesis can be used to mimic mobilization of human hematopoietic stem cells in vivo... - The fetal sheep: a unique model system for assessing the full differentiative potential of human stem cellsGraca Almeida Porada
Department of Animal Biotechnology, University of Nevada Reno, Reno 89557 0104, USA
Yonsei Med J 45:7-14. 2004.... - Persistent circulating human insulin in sheep transplanted in utero with human mesenchymal stem cellsAdel Ersek
Department of Animal Biotechnology, University of Nevada Reno, Reno, NV, USA
Exp Hematol 38:311-20. 2010..To determine if mesenchymal stem cells (MSC) derived from human fetal pancreatic tissue (pMSC) would engraft and differentiate in sheep pancreas following transplantation in utero... - Differentiative potential of human metanephric mesenchymal cellsGraca Almeida-Porada
Department of Animal Biotechnology, University of Nevada Reno, Mail Stop 202, Reno, NV 89557 0104, USA
Exp Hematol 30:1454-62. 2002..To evaluate the ability of mesenchymal cells derived from nonhematopoietic organs to form blood and other tissues in vitro and in vivo... - Transduction of long-term-engrafting human hematopoietic stem cells by retroviral vectorsChristopher D Porada
Department of Animal Biotechnology, University of Nevada, Reno, NV 89520, USA
Hum Gene Ther 13:867-79. 2002..The successful in vivo transduction of long-term engrafting human HSCs with the existing generation of murine retroviral vectors has significant implications for developing new approaches to pre- and postnatal gene therapy... 
Differences amid bone marrow and cord blood hematopoietic stem/progenitor cell division kineticsCláudia Lobato da Silva
Department of Animal Biotechnology, University of Nevada, Reno, Nevada 89557 0104, USA
J Cell Physiol 220:102-11. 2009....- Efficient generation of human hepatocytes by the intrahepatic delivery of clonal human mesenchymal stem cells in fetal sheepJason Chamberlain
Department of Animal Biotechnology, University of Nevada at Reno, Reno, NV 89557 0104, USA
Hepatology 46:1935-45. 2007.... - Generation of tissue-specific cells from MSC does not require fusion or donor-to-host mitochondrial/membrane transferEvan J Colletti
Department of Animal Biotechnology, University of Nevada at Reno, Reno, NV 89557, USA
Stem Cell Res 2:125-38. 2009.... - Bone marrow stem cells and liver regenerationGraca Almeida-Porada
Department of Animal Biotechnology, University of Nevada, Reno, Reno, NV 89557 0104, USA
Exp Hematol 38:574-80. 2010..Here, we will summarize the ability of each of these stem cell populations to give rise to functional hepatic elements that could mediate repair in patients with liver damage/disease... - In vivo generation of beta-cell-like cells from CD34(+) cells differentiated from human embryonic stem cellsA Daisy Goodrich
Department of Animal Biotechnology, University of Nevada, Reno, Reno, NV 89557, USA
Exp Hematol 38:516-525.e4. 2010..Based on this observation, we explored the capacity of CD34(+) cells derived in culture from the differentiation of human embryonic stem cells (hESC), for their in vivo pancreatic endocrine capacity... - Early fetal gene delivery utilizes both central and peripheral mechanisms of tolerance inductionEvan Colletti
Department of Animal Biotechnology, University of Nevada, Reno, NV 89557 0104, USA
Exp Hematol 36:816-22. 2008..In the present studies, we conduct detailed analysis of the thymus of recipients of in utero gene transfer (IUGT) to delineate the mechanism of the observed immune tolerance and assess the impact of recipient age on this process... - The sheep model of in utero gene therapyChristopher D Porada
Department of Animal Biotechnology, University of Nevada, Reno, Nev 89557 0104, USA
Fetal Diagn Ther 19:23-30. 2004.... - Human mesenchymal stem cells form Purkinje fibers in fetal sheep heartJudith A Airey
Department of Pharmacology, MS 318, University of Nevada, Reno, Reno, NV 89523, USA
Circulation 109:1401-7. 2004..We have investigated the usefulness of a model of cardiac development in a large mammal, sheep, for studies of engraftment of human stem cells in the heart... - Formation of human hepatocytes by human hematopoietic stem cells in sheepGraca Almeida-Porada
Department of Animal Biotechnology, University of Nevada Reno, Mail Stop 202, Reno, NV 89557 0104, USA
Blood 104:2582-90. 2004.... - Gestational age of recipient determines pattern and level of transgene expression following in utero retroviral gene transferChristopher D Porada
Department of Animal Biotechnology, University of Nevada at Reno, Reno, NV 89557, USA
Mol Ther 11:284-93. 2005..Our results suggest that with further vector optimization this approach may be useful for treating diseases that involve the lung and liver early in development... - Adult mesenchymal stem cells: a pluripotent population with multiple applicationsChristopher D Porada
Department of Animal Biotechnology, University of Nevada, Reno 89557-0104, USA
Curr Stem Cell Res Ther 1:365-9. 2006..In this review we summarize the latest research in the use of mesenchymal stem cells in transplantation for generalized diseases, local implantation for local tissue defects, and as a vehicle for genes in gene therapy protocols... - Generation of functional natural killer and dendritic cells in a human stromal-based serum-free culture system designed for cord blood expansionAna M Frias
Department of Animal Biotechnology, University of Nevada, Reno 89557 0104, USA
Exp Hematol 36:61-8. 2008..In this study, we investigated the ability of the CD7(+)CD34(-) population to differentiate into natural killer and dendritic cells (DCs)... - Docosahexaenoic acid induces dose dependent cell death in an early undifferentiated subtype of acute myeloid leukemia cell lineTakashi Yamagami
Department of Animal Biotechnology, University of Nevada, Reno, NV 89557 0104, USA
Cancer Biol Ther 8:331-7. 2009..Since we also show that DHA does not have a detrimental effect on normal hematopoiesis our results suggest that DHA could potentially serve as an well-tolerated adjuvant in the treatment of AML patients... - Retroviral transduction of IL2RG into CD34(+) cells from X-linked severe combined immunodeficiency patients permits human T- and B-cell development in sheep chimerasEmily J Tsai
National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
Blood 100:72-9. 2002..These results suggest that gene transfer to autologous peripheral CD34(+) cells using MFGS-gc retrovirus may benefit XSCID patients with persistent T- and B-cell deficits despite prior BMT... - Improved transduction of human sheep repopulating cells by retrovirus vectors pseudotyped with feline leukemia virus type C or RD114 envelopesM Lee Lucas
Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
Blood 106:51-8. 2005....
Research Grants
- IN UTERO GENE THERAPYChristopher Porada; Fiscal Year: 2006..These studies in this unique fetal model may lay the groundwork for devising clinically relevant in itero GT approaches for treating a variety of genetic diseases in human fetuses early in gestation, prior to disease onset. ..