Christopher Porada

Summary

Affiliation: University of Nevada
Country: USA

Publications

  1. pmc Mesenchymal stem cells as therapeutics and vehicles for gene and drug delivery
    Christopher D Porada
    Department of Animal Biotechnology, University of Nevada, Reno, 89557 0104, USA
    Adv Drug Deliv Rev 62:1156-66. 2010
  2. pmc Development and characterization of a novel CD34 monoclonal antibody that identifies sheep hematopoietic stem/progenitor cells
    Christopher D Porada
    Department of Animal Biotechnology, School of Veterinary Medicine, University of Nevada, Reno, NV 89557 0104, USA
    Exp Hematol 36:1739-49. 2008
  3. ncbi request reprint Male germ-line cells are at risk following direct-injection retroviral-mediated gene transfer in utero
    Christopher D Porada
    Department of Animal Biotechnology, University of Nevada at Reno, 89557 0104, USA
    Mol Ther 12:754-62. 2005
  4. ncbi request reprint Plasticity of human stem cells in the fetal sheep model of human stem cell transplantation
    Graca Almeida-Porada
    Department of Animal Biotechnology, University of Nevada, Reno, Nevada 89557 0104, USA
    Int J Hematol 79:1-6. 2004
  5. pmc Factors determining the risk of inadvertent retroviral transduction of male germ cells after in utero gene transfer in sheep
    Paul J Park
    Department of Animal Biotechnology, School of Veterinary Medicine, University of Nevada, Reno, NV 89557, USA
    Hum Gene Ther 20:201-15. 2009
  6. pmc The human-sheep chimeras as a model for human stem cell mobilization and evaluation of hematopoietic grafts' potential
    Graca Almeida-Porada
    Department of Animal Biotechnology University of Nevada, Reno, NV 89557 0104, USA
    Exp Hematol 35:1594-600. 2007
  7. ncbi request reprint The fetal sheep: a unique model system for assessing the full differentiative potential of human stem cells
    Graca Almeida Porada
    Department of Animal Biotechnology, University of Nevada Reno, Reno 89557 0104, USA
    Yonsei Med J 45:7-14. 2004
  8. pmc Persistent circulating human insulin in sheep transplanted in utero with human mesenchymal stem cells
    Adel Ersek
    Department of Animal Biotechnology, University of Nevada Reno, Reno, NV, USA
    Exp Hematol 38:311-20. 2010
  9. ncbi request reprint Transduction of long-term-engrafting human hematopoietic stem cells by retroviral vectors
    Christopher D Porada
    Department of Animal Biotechnology, University of Nevada, Reno, NV 89520, USA
    Hum Gene Ther 13:867-79. 2002
  10. doi request reprint Differences amid bone marrow and cord blood hematopoietic stem/progenitor cell division kinetics
    Cláudia Lobato da Silva
    Department of Animal Biotechnology, University of Nevada, Reno, Nevada 89557 0104, USA
    J Cell Physiol 220:102-11. 2009

Research Grants

  1. IN UTERO GENE THERAPY
    Christopher Porada; Fiscal Year: 2006

Collaborators

Detail Information

Publications25

  1. pmc Mesenchymal stem cells as therapeutics and vehicles for gene and drug delivery
    Christopher D Porada
    Department of Animal Biotechnology, University of Nevada, Reno, 89557 0104, USA
    Adv Drug Deliv Rev 62:1156-66. 2010
    ....
  2. pmc Development and characterization of a novel CD34 monoclonal antibody that identifies sheep hematopoietic stem/progenitor cells
    Christopher D Porada
    Department of Animal Biotechnology, School of Veterinary Medicine, University of Nevada, Reno, NV 89557 0104, USA
    Exp Hematol 36:1739-49. 2008
    ..The current studies were undertaken to create a monoclonal antibody to sheep CD34 that would enable isolation and study of sheep HSC/progenitors...
  3. ncbi request reprint Male germ-line cells are at risk following direct-injection retroviral-mediated gene transfer in utero
    Christopher D Porada
    Department of Animal Biotechnology, University of Nevada at Reno, 89557 0104, USA
    Mol Ther 12:754-62. 2005
    ..In conclusion, our analysis on over 3 x 10(9) sperm cells suggests that the direct-injection approach employed in these studies may result in the inadvertent transduction of very low numbers of male germ cells...
  4. ncbi request reprint Plasticity of human stem cells in the fetal sheep model of human stem cell transplantation
    Graca Almeida-Porada
    Department of Animal Biotechnology, University of Nevada, Reno, Nevada 89557 0104, USA
    Int J Hematol 79:1-6. 2004
    ..In this review, we describe the advantages of the permissive aspects of the developing preimmune fetus in the early gestational age that led us to develop the sheep as a large-animal model of human stem cell plasticity...
  5. pmc Factors determining the risk of inadvertent retroviral transduction of male germ cells after in utero gene transfer in sheep
    Paul J Park
    Department of Animal Biotechnology, School of Veterinary Medicine, University of Nevada, Reno, NV 89557, USA
    Hum Gene Ther 20:201-15. 2009
    ....
  6. pmc The human-sheep chimeras as a model for human stem cell mobilization and evaluation of hematopoietic grafts' potential
    Graca Almeida-Porada
    Department of Animal Biotechnology University of Nevada, Reno, NV 89557 0104, USA
    Exp Hematol 35:1594-600. 2007
    ..To investigate whether the sheep xenograft model of human hematopoiesis can be used to mimic mobilization of human hematopoietic stem cells in vivo...
  7. ncbi request reprint The fetal sheep: a unique model system for assessing the full differentiative potential of human stem cells
    Graca Almeida Porada
    Department of Animal Biotechnology, University of Nevada Reno, Reno 89557 0104, USA
    Yonsei Med J 45:7-14. 2004
    ....
  8. pmc Persistent circulating human insulin in sheep transplanted in utero with human mesenchymal stem cells
    Adel Ersek
    Department of Animal Biotechnology, University of Nevada Reno, Reno, NV, USA
    Exp Hematol 38:311-20. 2010
    ..To determine if mesenchymal stem cells (MSC) derived from human fetal pancreatic tissue (pMSC) would engraft and differentiate in sheep pancreas following transplantation in utero...
  9. ncbi request reprint Transduction of long-term-engrafting human hematopoietic stem cells by retroviral vectors
    Christopher D Porada
    Department of Animal Biotechnology, University of Nevada, Reno, NV 89520, USA
    Hum Gene Ther 13:867-79. 2002
    ..The successful in vivo transduction of long-term engrafting human HSCs with the existing generation of murine retroviral vectors has significant implications for developing new approaches to pre- and postnatal gene therapy...
  10. doi request reprint Differences amid bone marrow and cord blood hematopoietic stem/progenitor cell division kinetics
    Cláudia Lobato da Silva
    Department of Animal Biotechnology, University of Nevada, Reno, Nevada 89557 0104, USA
    J Cell Physiol 220:102-11. 2009
    ....
  11. ncbi request reprint Differentiative potential of human metanephric mesenchymal cells
    Graca Almeida-Porada
    Department of Animal Biotechnology, University of Nevada Reno, Mail Stop 202, Reno, NV 89557 0104, USA
    Exp Hematol 30:1454-62. 2002
    ..To evaluate the ability of mesenchymal cells derived from nonhematopoietic organs to form blood and other tissues in vitro and in vivo...
  12. ncbi request reprint Efficient generation of human hepatocytes by the intrahepatic delivery of clonal human mesenchymal stem cells in fetal sheep
    Jason Chamberlain
    Department of Animal Biotechnology, University of Nevada at Reno, Reno, NV 89557 0104, USA
    Hepatology 46:1935-45. 2007
    ..Furthermore, hepatocytes were generated from MSCs without the need to first migrate/lodge to the bone marrow and give rise to hematopoietic cells...
  13. doi request reprint Generation of tissue-specific cells from MSC does not require fusion or donor-to-host mitochondrial/membrane transfer
    Evan J Colletti
    Department of Animal Biotechnology, University of Nevada at Reno, Reno, NV 89557, USA
    Stem Cell Res 2:125-38. 2009
    ....
  14. pmc Bone marrow stem cells and liver regeneration
    Graca Almeida-Porada
    Department of Animal Biotechnology, University of Nevada, Reno, Reno, NV 89557 0104, USA
    Exp Hematol 38:574-80. 2010
    ..Here, we will summarize the ability of each of these stem cell populations to give rise to functional hepatic elements that could mediate repair in patients with liver damage/disease...
  15. pmc In vivo generation of beta-cell-like cells from CD34(+) cells differentiated from human embryonic stem cells
    A Daisy Goodrich
    Department of Animal Biotechnology, University of Nevada, Reno, Reno, NV 89557, USA
    Exp Hematol 38:516-525.e4. 2010
    ..Based on this observation, we explored the capacity of CD34(+) cells derived in culture from the differentiation of human embryonic stem cells (hESC), for their in vivo pancreatic endocrine capacity...
  16. pmc Early fetal gene delivery utilizes both central and peripheral mechanisms of tolerance induction
    Evan Colletti
    Department of Animal Biotechnology, University of Nevada, Reno, NV 89557 0104, USA
    Exp Hematol 36:816-22. 2008
    ..In the present studies, we conduct detailed analysis of the thymus of recipients of in utero gene transfer (IUGT) to delineate the mechanism of the observed immune tolerance and assess the impact of recipient age on this process...
  17. ncbi request reprint The sheep model of in utero gene therapy
    Christopher D Porada
    Department of Animal Biotechnology, University of Nevada, Reno, Nev 89557 0104, USA
    Fetal Diagn Ther 19:23-30. 2004
    ....
  18. ncbi request reprint Human mesenchymal stem cells form Purkinje fibers in fetal sheep heart
    Judith A Airey
    Department of Pharmacology, MS 318, University of Nevada, Reno, Reno, NV 89523, USA
    Circulation 109:1401-7. 2004
    ..We have investigated the usefulness of a model of cardiac development in a large mammal, sheep, for studies of engraftment of human stem cells in the heart...
  19. ncbi request reprint Formation of human hepatocytes by human hematopoietic stem cells in sheep
    Graca Almeida-Porada
    Department of Animal Biotechnology, University of Nevada Reno, Mail Stop 202, Reno, NV 89557 0104, USA
    Blood 104:2582-90. 2004
    ....
  20. ncbi request reprint Gestational age of recipient determines pattern and level of transgene expression following in utero retroviral gene transfer
    Christopher D Porada
    Department of Animal Biotechnology, University of Nevada at Reno, Reno, NV 89557, USA
    Mol Ther 11:284-93. 2005
    ..Our results suggest that with further vector optimization this approach may be useful for treating diseases that involve the lung and liver early in development...
  21. ncbi request reprint Adult mesenchymal stem cells: a pluripotent population with multiple applications
    Christopher D Porada
    Department of Animal Biotechnology, University of Nevada, Reno 89557 0104, USA
    Curr Stem Cell Res Ther 1:365-9. 2006
    ..In this review we summarize the latest research in the use of mesenchymal stem cells in transplantation for generalized diseases, local implantation for local tissue defects, and as a vehicle for genes in gene therapy protocols...
  22. pmc Generation of functional natural killer and dendritic cells in a human stromal-based serum-free culture system designed for cord blood expansion
    Ana M Frias
    Department of Animal Biotechnology, University of Nevada, Reno 89557 0104, USA
    Exp Hematol 36:61-8. 2008
    ..In this study, we investigated the ability of the CD7(+)CD34(-) population to differentiate into natural killer and dendritic cells (DCs)...
  23. ncbi request reprint Docosahexaenoic acid induces dose dependent cell death in an early undifferentiated subtype of acute myeloid leukemia cell line
    Takashi Yamagami
    Department of Animal Biotechnology, University of Nevada, Reno, NV 89557 0104, USA
    Cancer Biol Ther 8:331-7. 2009
    ..Since we also show that DHA does not have a detrimental effect on normal hematopoiesis our results suggest that DHA could potentially serve as an well-tolerated adjuvant in the treatment of AML patients...
  24. ncbi request reprint Retroviral transduction of IL2RG into CD34(+) cells from X-linked severe combined immunodeficiency patients permits human T- and B-cell development in sheep chimeras
    Emily J Tsai
    National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 100:72-9. 2002
    ..These results suggest that gene transfer to autologous peripheral CD34(+) cells using MFGS-gc retrovirus may benefit XSCID patients with persistent T- and B-cell deficits despite prior BMT...
  25. pmc Improved transduction of human sheep repopulating cells by retrovirus vectors pseudotyped with feline leukemia virus type C or RD114 envelopes
    M Lee Lucas
    Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 106:51-8. 2005
    ....

Research Grants5

  1. IN UTERO GENE THERAPY
    Christopher Porada; Fiscal Year: 2006
    ..These studies in this unique fetal model may lay the groundwork for devising clinically relevant in itero GT approaches for treating a variety of genetic diseases in human fetuses early in gestation, prior to disease onset. ..