Research Topics
| STEPHEN POLYAKSummaryAffiliation: University of Washington Country: USA Publications
Research Grants
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Detail Information
Publications
Inhibition of T-cell inflammatory cytokines, hepatocyte NF-kappaB signaling, and HCV infection by standardized SilymarinStephen J Polyak
Department of Laboratory Medicine, University of Washington, Seattle, Washington 98104 2499, USA
Gastroenterology 132:1925-36. 2007..Milk thistle's clinical benefits for chronic hepatitis C are unsettled due to variability in standardization of the herbal product...
Arbidol: a broad-spectrum antiviral that inhibits acute and chronic HCV infectionYury S Boriskin
Departments of Laboratory Medicine, Microbiology and Pathobiology, University of Washington, Seattle, USA
Virol J 3:56. 2006..ARB's affinity for membranes may inhibit several aspects of the HCV lifecycle that are membrane-dependent...- Effect of ethanol on innate antiviral pathways and HCV replication in human liver cellsCourtney R Plumlee
Department of Laboratory Medicine, University of Washington, Seattle, USA
Virol J 2:89. 2005..To begin to address these issues, we characterized the interaction of acute ethanol on Jak-Stat and MAPK pathways in Huh7 cells, HCV replicon cells lines, and primary human hepatocytes... - Comparison of amplification enzymes for Hepatitis C Virus quasispecies analysisStephen J Polyak
Virology Division, Department of Laboratory Medicine, University of Washington, Seattle, WA, USA
Virol J 2:41. 2005..QS diversity and complexity were quantified using heteroduplex mobility assays (HMA)... - Hepatitis C virus--cell interactions and their role in pathogenesisStephen J Polyak
Department of Laboratory Medicine, University of Washington, Box 359690, 325 9th Avenue, Seattle, WA 98104 2499, USA
Clin Liver Dis 7:67-88. 2003.... - Characterization of the effects of hepatitis C virus nonstructural 5A protein expression in human cell lines and on interferon-sensitive virus replicationS J Polyak
Department of Laboratory Medicine, University of Washington, Seattle, WA, USA
Hepatology 29:1262-71. 1999..A scenario is discussed in which the NS5A protein may employ multiple strategies contributing to IFN resistance during HCV infection... - The protein kinase-interacting domain in the hepatitis C virus envelope glycoprotein-2 gene is highly conserved in genotype 1-infected patients treated with interferonS J Polyak
Virology Division, University of Washington, Seattle, WA 98104 2499, USA
J Infect Dis 182:397-404. 2000..The PePHD is highly conserved in HCV-1-infected IFN responders and nonresponders and does not appear to evolve in response to IFN therapy... 
Hepatitis C virus nonstructural 5A protein induces interleukin-8, leading to partial inhibition of the interferon-induced antiviral responseS J Polyak
Department of Laboratory Medicine, Virology Division, University of Washington, 325 9th Ave, Seattle, WA 98104 2499, USA
J Virol 75:6095-106. 2001..During HCV infection, viral proteins may induce chemokines that contribute to HCV antiviral resistance and pathogenesis...- Elevated levels of interleukin-8 in serum are associated with hepatitis C virus infection and resistance to interferon therapyS J Polyak
Department of Laboratory Medicine, Virology Division, University of Washington, 325 9th Ave, Seattle, WA 98104 2499, USA
J Virol 75:6209-11. 2001..This study suggests that HCV-induced changes in levels of chemokine and cytokine expression may be involved in HCV antiviral resistance, persistence, and pathogenesis... - Identification of hepatoprotective flavonolignans from silymarinStephen J Polyak
Department of Laboratory Medicine, Microbiology and Global Health, University of Washington, Seattle, WA 98104, USA
Proc Natl Acad Sci U S A 107:5995-9. 2010..The data suggest that silymarin- and silymarin-derived compounds may influence HCV disease course in some patients. Studies where standardized silymarin is dosed to identify specific clinical endpoints are urgently needed... - Relationships between hepatitis C virus replication and CXCL-8 production in vitroBon Chang A Koo
Virology Division, Department of Laboratory Medicine, 359690, 325 9th Avenue, Seattle, WA 98104 2499, USA
J Virol 80:7885-93. 2006.... - Hepatitis C virus-specific immune responses and quasi-species variability at baseline are associated with nonresponse to antiviral therapy during advanced hepatitis CChihiro Morishima
Department of Laboratory Medicine and Pediatrics, University of Washington, Seattle, USA
J Infect Dis 193:931-40. 2006..Thus, in patients with advanced hepatic fibrosis due to HCV infection, pretreatment HCV-specific immune responses and increased QS variability appear to hinder viral clearance by pegylated IFN- alpha 2a and ribavirin combination therapy... - Silymarin inhibits in vitro T-cell proliferation and cytokine production in hepatitis C virus infectionChihiro Morishima
Division of Virology, Department of Laboratory Medicine, University of Washington School of Medicine, Seattle, Washington 98104, USA
Gastroenterology 138:671-81, 681.e1-2. 2010..The goal of this study was to determine whether silymarin has in vitro effects on immune function that might have implications for its potential effect on hepatitis C virus (HCV)-induced liver disease... - Hepatitis C virus induces oxidative stress, DNA damage and modulates the DNA repair enzyme NEIL1Sampa Pal
Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195 7110 USA
J Gastroenterol Hepatol 25:627-34. 2010..In the current study we examined the induction of reactive oxygen species (ROS) resulting from HCV infection and evaluated its effect on the host DNA damage and repair machinery... - Multiple effects of silymarin on the hepatitis C virus lifecycleJessica Wagoner
Department of Laboratory Medicine, University of Washington, Seattle, WA 98104 2499, USA
Hepatology 51:1912-21. 2010..CONCLUSION: Although inhibition of in vitro NS5B polymerase activity is demonstrable, the mechanisms of silymarin's antiviral action appear to include blocking of virus entry and transmission, possibly by targeting the host cell... - Functional characterization of core genes from patients with acute hepatitis C virus infectionXi Tang
Departments of Laboratory Medicine, University of Washington, Seattle, WA 98104, USA
J Infect Dis 201:912-22. 2010..The hepatitis C virus (HCV) core protein is implicated in diverse aspects of HCV-induced pathogenesis. There is a paucity of information on core in acute hepatitis C infection... - Stability of CXCL-8 and related AU-rich mRNAs in the context of hepatitis C virus replication in vitroJamison Green
Department of Laboratory Medicine, University of Washington, Seattle, 98104-2499, USA
J Infect Dis 193:802-11. 2006..CONCLUSION: The data suggest that increased CXCL-8 gene expression in the context of HCV replication involves posttranscriptional events... - Unique features of hepatitis C virus capsid formation revealed by de novo cell-free assemblyKevin C Klein
Department of Pathobiology, University of Washington, Seattle, Washington 98195, USA
J Virol 78:9257-69. 2004..By allowing reproducible and quantitative assessment of viral and cellular requirements for capsid formation, these cell-free systems make a mechanistic dissection of HCV capsid assembly possible... - Effects of the hepatitis C virus core protein on innate cellular defense pathwaysKristen Miller
Virology Division, Department of Laboratory Medicine, University of Washington, Seattle, WA 98104-2499, USA
J Interferon Cytokine Res 24:391-402. 2004..Core stimulated the human iNOS promoter and induced iNOS protein. The data indicate that HCV core can modulate IRF, Jak-Stat, and iNOS pathways and suggest mechanisms by which core could affect HCV persistence and pathogenesis... - Regulation of CXCL-8 (interleukin-8) induction by double-stranded RNA signaling pathways during hepatitis C virus infectionJessica Wagoner
Department of Laboratory Medicine, University of Washington, Virology 359690, 325 9th Avenue, Seattle, WA 98104 2499, USA
J Virol 81:309-18. 2007.... - Subversion of cell signaling pathways by hepatitis C virus nonstructural 5A protein via interaction with Grb2 and P85 phosphatidylinositol 3-kinaseYupeng He
Department of Microbiology, School of Medicine, University of Washington, Seattle, Washington 98195, USA
J Virol 76:9207-17. 2002.... - Hepatitis C virus NS5A colocalizes with the core protein on lipid droplets and interacts with apolipoproteinsStephanie T Shi
Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, California 90033, USA
Virology 292:198-210. 2002.... - Hepatitis C virus-host interactions: the NS5A protein and the interferon/chemokine systemsKhalid S A Khabar
Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Centre, Riyadh, Kingdom of Saudi Arabia
J Interferon Cytokine Res 22:1005-12. 2002..In this review, we focus on the HCV nonstructural 5A (NS5A) protein, a model for diverse virus-host interactions, and highlight the interaction of viruses, including HCV, with the chemokine system... - Antiviral effects of silymarin against hepatitis C: the jury is still outStephen J Polyak
Hepatology 48:345-6. 2008 - Mechanisms of action of interferon and ribavirin in chronic hepatitis C: Summary of a workshopRaymond T Chung
Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
Hepatology 47:306-20. 2008 - Associations among clinical, immunological, and viral quasispecies measurements in advanced chronic hepatitis CAlan Lee Rothman
Center for Infectious Disease and Vaccine Research and Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA
Hepatology 41:617-25. 2005.... - Detection of hepatitis C virus RNA in normal cervical smearsChia Wang
Clin Infect Dis 37:314; author reply 314-5. 2003 - Biochemical mechanism of hepatitis C virus inhibition by the broad-spectrum antiviral arbidolEve Isabelle Pécheur
IFR128 Biosciences Lyon Gerland, Institut de Biologie et Chimie des Proteines, UMR 5086 CNRS Université Claude Bernard Lyon I, 7 passage du Vercors, 69367 Lyon Cedex 07, France e pecheur ibcp fr
Biochemistry 46:6050-9. 2007..Our results suggest that the affinity of ARB for lipid membranes could account for its anti-HCV actions, together with a differential level of interaction with key motifs in HCV glycoproteins of different genotypes... - Hepatitis C infection and alcohol use: A dangerous mix for the liver and antiviral immunityGyongyi Szabo
Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
Alcohol Clin Exp Res 30:709-19. 2006..Ethanol on Innate Antiviral Signaling Pathways, Hepatitis C Replication, and Human Liver Cell Transcription by Stephen Polyak; (3) Ethanol Alters Dendritic Cell Function In Vivo and Impairs the Subsequent Cellular Immune Responses to .. - Expressed gene clusters associated with cellular sensitivity and resistance towards anti-viral and anti-proliferative actions of interferonKhalid S A Khabar
Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Center, Riyadh 11211, Saudi Arabia
J Mol Biol 342:833-46. 2004..Use of rationally designed microarray experiments in IFN biology yielded informative clusters that may provide candidate genes for diagnostic or for evaluation of therapeutic possibilities... - Engulfment of apoptotic cells expressing HCV proteins leads to differential chemokine expression and STAT signaling in human dendritic cellsAnne M Wertheimer
Department of Medicine, Portland VAMC and Oregon Health and Science University, Portland, OR, USA
Hepatology 45:1422-32. 2007..CONCLUSION: These data provide novel mechanisms by which HCV subverts antiviral host immunity...
Research Grants
- Hepatitis C Virus Proteins and EthanolSTEPHEN POLYAK; Fiscal Year: 2004..abstract_text> ..
- Hepatitis C Virus Induced IL-8 & Inhibition of InterferonSTEPHEN POLYAK; Fiscal Year: 2007..Moreover, IL-8 or its receptors may prove to be suitable targets for therapeutic intervention in chronic hepatitis C. ..