STEPHEN POLYAK

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. ncbi request reprint Inhibition of T-cell inflammatory cytokines, hepatocyte NF-kappaB signaling, and HCV infection by standardized Silymarin
    Stephen J Polyak
    Department of Laboratory Medicine, University of Washington, Seattle, Washington 98104 2499, USA
    Gastroenterology 132:1925-36. 2007
  2. ncbi request reprint Arbidol: a broad-spectrum antiviral compound that blocks viral fusion
    Y S Boriskin
    Institute of Virology, Moscow, Russia
    Curr Med Chem 15:997-1005. 2008
  3. pmc Arbidol: a broad-spectrum antiviral that inhibits acute and chronic HCV infection
    Yury S Boriskin
    Departments of Laboratory Medicine, Microbiology and Pathobiology, University of Washington, Seattle, USA
    Virol J 3:56. 2006
  4. pmc Effect of ethanol on innate antiviral pathways and HCV replication in human liver cells
    Courtney R Plumlee
    Department of Laboratory Medicine, University of Washington, Seattle, USA
    Virol J 2:89. 2005
  5. pmc Comparison of amplification enzymes for Hepatitis C Virus quasispecies analysis
    Stephen J Polyak
    Virology Division, Department of Laboratory Medicine, University of Washington, Seattle, WA, USA
    Virol J 2:41. 2005
  6. ncbi request reprint Hepatitis C virus--cell interactions and their role in pathogenesis
    Stephen J Polyak
    Department of Laboratory Medicine, University of Washington, Box 359690, 325 9th Avenue, Seattle, WA 98104 2499, USA
    Clin Liver Dis 7:67-88. 2003
  7. pmc Identification of hepatoprotective flavonolignans from silymarin
    Stephen J Polyak
    Department of Laboratory Medicine, Microbiology and Global Health, University of Washington, Seattle, WA 98104, USA
    Proc Natl Acad Sci U S A 107:5995-9. 2010
  8. pmc Elevated levels of interleukin-8 in serum are associated with hepatitis C virus infection and resistance to interferon therapy
    S J Polyak
    Department of Laboratory Medicine, Virology Division, University of Washington, 325 9th Ave, Seattle, WA 98104 2499, USA
    J Virol 75:6209-11. 2001
  9. pmc Hepatitis C virus nonstructural 5A protein induces interleukin-8, leading to partial inhibition of the interferon-induced antiviral response
    S J Polyak
    Department of Laboratory Medicine, Virology Division, University of Washington, 325 9th Ave, Seattle, WA 98104 2499, USA
    J Virol 75:6095-106. 2001
  10. ncbi request reprint The protein kinase-interacting domain in the hepatitis C virus envelope glycoprotein-2 gene is highly conserved in genotype 1-infected patients treated with interferon
    S J Polyak
    Virology Division, University of Washington, Seattle, WA 98104 2499, USA
    J Infect Dis 182:397-404. 2000

Research Grants

  1. Hepatitis C Virus Proteins and Ethanol
    STEPHEN POLYAK; Fiscal Year: 2004
  2. Hepatitis C Virus Induced IL-8 & Inhibition of Interferon
    STEPHEN POLYAK; Fiscal Year: 2007

Collaborators

Detail Information

Publications32

  1. ncbi request reprint Inhibition of T-cell inflammatory cytokines, hepatocyte NF-kappaB signaling, and HCV infection by standardized Silymarin
    Stephen J Polyak
    Department of Laboratory Medicine, University of Washington, Seattle, Washington 98104 2499, USA
    Gastroenterology 132:1925-36. 2007
    ..Milk thistle's clinical benefits for chronic hepatitis C are unsettled due to variability in standardization of the herbal product...
  2. ncbi request reprint Arbidol: a broad-spectrum antiviral compound that blocks viral fusion
    Y S Boriskin
    Institute of Virology, Moscow, Russia
    Curr Med Chem 15:997-1005. 2008
    ....
  3. pmc Arbidol: a broad-spectrum antiviral that inhibits acute and chronic HCV infection
    Yury S Boriskin
    Departments of Laboratory Medicine, Microbiology and Pathobiology, University of Washington, Seattle, USA
    Virol J 3:56. 2006
    ..ARB's affinity for membranes may inhibit several aspects of the HCV lifecycle that are membrane-dependent...
  4. pmc Effect of ethanol on innate antiviral pathways and HCV replication in human liver cells
    Courtney R Plumlee
    Department of Laboratory Medicine, University of Washington, Seattle, USA
    Virol J 2:89. 2005
    ..To begin to address these issues, we characterized the interaction of acute ethanol on Jak-Stat and MAPK pathways in Huh7 cells, HCV replicon cells lines, and primary human hepatocytes...
  5. pmc Comparison of amplification enzymes for Hepatitis C Virus quasispecies analysis
    Stephen J Polyak
    Virology Division, Department of Laboratory Medicine, University of Washington, Seattle, WA, USA
    Virol J 2:41. 2005
    ..QS diversity and complexity were quantified using heteroduplex mobility assays (HMA)...
  6. ncbi request reprint Hepatitis C virus--cell interactions and their role in pathogenesis
    Stephen J Polyak
    Department of Laboratory Medicine, University of Washington, Box 359690, 325 9th Avenue, Seattle, WA 98104 2499, USA
    Clin Liver Dis 7:67-88. 2003
    ....
  7. pmc Identification of hepatoprotective flavonolignans from silymarin
    Stephen J Polyak
    Department of Laboratory Medicine, Microbiology and Global Health, University of Washington, Seattle, WA 98104, USA
    Proc Natl Acad Sci U S A 107:5995-9. 2010
    ..The data suggest that silymarin- and silymarin-derived compounds may influence HCV disease course in some patients. Studies where standardized silymarin is dosed to identify specific clinical endpoints are urgently needed...
  8. pmc Elevated levels of interleukin-8 in serum are associated with hepatitis C virus infection and resistance to interferon therapy
    S J Polyak
    Department of Laboratory Medicine, Virology Division, University of Washington, 325 9th Ave, Seattle, WA 98104 2499, USA
    J Virol 75:6209-11. 2001
    ..This study suggests that HCV-induced changes in levels of chemokine and cytokine expression may be involved in HCV antiviral resistance, persistence, and pathogenesis...
  9. pmc Hepatitis C virus nonstructural 5A protein induces interleukin-8, leading to partial inhibition of the interferon-induced antiviral response
    S J Polyak
    Department of Laboratory Medicine, Virology Division, University of Washington, 325 9th Ave, Seattle, WA 98104 2499, USA
    J Virol 75:6095-106. 2001
    ..During HCV infection, viral proteins may induce chemokines that contribute to HCV antiviral resistance and pathogenesis...
  10. ncbi request reprint The protein kinase-interacting domain in the hepatitis C virus envelope glycoprotein-2 gene is highly conserved in genotype 1-infected patients treated with interferon
    S J Polyak
    Virology Division, University of Washington, Seattle, WA 98104 2499, USA
    J Infect Dis 182:397-404. 2000
    ..The PePHD is highly conserved in HCV-1-infected IFN responders and nonresponders and does not appear to evolve in response to IFN therapy...
  11. ncbi request reprint Characterization of the effects of hepatitis C virus nonstructural 5A protein expression in human cell lines and on interferon-sensitive virus replication
    S J Polyak
    Department of Laboratory Medicine, University of Washington, Seattle, WA, USA
    Hepatology 29:1262-71. 1999
    ..A scenario is discussed in which the NS5A protein may employ multiple strategies contributing to IFN resistance during HCV infection...
  12. pmc Silymarin inhibits in vitro T-cell proliferation and cytokine production in hepatitis C virus infection
    Chihiro Morishima
    Division of Virology, Department of Laboratory Medicine, University of Washington School of Medicine, Seattle, Washington 98104, USA
    Gastroenterology 138:671-81, 681.e1-2. 2010
    ..The goal of this study was to determine whether silymarin has in vitro effects on immune function that might have implications for its potential effect on hepatitis C virus (HCV)-induced liver disease...
  13. pmc Hepatitis C virus induces oxidative stress, DNA damage and modulates the DNA repair enzyme NEIL1
    Sampa Pal
    Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195 7110 USA
    J Gastroenterol Hepatol 25:627-34. 2010
    ..In the current study we examined the induction of reactive oxygen species (ROS) resulting from HCV infection and evaluated its effect on the host DNA damage and repair machinery...
  14. pmc Relationships between hepatitis C virus replication and CXCL-8 production in vitro
    Bon Chang A Koo
    Virology Division, Department of Laboratory Medicine, 359690, 325 9th Avenue, Seattle, WA 98104 2499, USA
    J Virol 80:7885-93. 2006
    ....
  15. ncbi request reprint Hepatitis C virus-specific immune responses and quasi-species variability at baseline are associated with nonresponse to antiviral therapy during advanced hepatitis C
    Chihiro Morishima
    Department of Laboratory Medicine and Pediatrics, University of Washington, Seattle, USA
    J Infect Dis 193:931-40. 2006
    ..Thus, in patients with advanced hepatic fibrosis due to HCV infection, pretreatment HCV-specific immune responses and increased QS variability appear to hinder viral clearance by pegylated IFN- alpha 2a and ribavirin combination therapy...
  16. pmc Multiple effects of silymarin on the hepatitis C virus lifecycle
    Jessica Wagoner
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98104 2499, USA
    Hepatology 51:1912-21. 2010
    ..Silymarin inhibited microsomal triglyceride transfer protein activity, apolipoprotein B secretion, and infectious virion production into culture supernatants. Silymarin also blocked cell-to-cell spread of virus...
  17. pmc Functional characterization of core genes from patients with acute hepatitis C virus infection
    Xi Tang
    Departments of Laboratory Medicine, University of Washington, Seattle, WA 98104, USA
    J Infect Dis 201:912-22. 2010
    ..The hepatitis C virus (HCV) core protein is implicated in diverse aspects of HCV-induced pathogenesis. There is a paucity of information on core in acute hepatitis C infection...
  18. ncbi request reprint Stability of CXCL-8 and related AU-rich mRNAs in the context of hepatitis C virus replication in vitro
    Jamison Green
    Department of Laboratory Medicine, University of Washington, Seattle, 98104 2499, USA
    J Infect Dis 193:802-11. 2006
    ..CXCL-8 mRNA stability was, therefore, investigated in the context of HCV replication in 4 replicon cell lines, Huh7 and Huh7.5 cells, and primary human fetal hepatocytes...
  19. pmc Subversion of cell signaling pathways by hepatitis C virus nonstructural 5A protein via interaction with Grb2 and P85 phosphatidylinositol 3-kinase
    Yupeng He
    Department of Microbiology, School of Medicine, University of Washington, Seattle, Washington 98195, USA
    J Virol 76:9207-17. 2002
    ....
  20. pmc Unique features of hepatitis C virus capsid formation revealed by de novo cell-free assembly
    Kevin C Klein
    Department of Pathobiology, University of Washington, Seattle, Washington 98195, USA
    J Virol 78:9257-69. 2004
    ..By allowing reproducible and quantitative assessment of viral and cellular requirements for capsid formation, these cell-free systems make a mechanistic dissection of HCV capsid assembly possible...
  21. pmc Regulation of CXCL-8 (interleukin-8) induction by double-stranded RNA signaling pathways during hepatitis C virus infection
    Jessica Wagoner
    Department of Laboratory Medicine, University of Washington, Virology 359690, 325 9th Avenue, Seattle, WA 98104 2499, USA
    J Virol 81:309-18. 2007
    ....
  22. ncbi request reprint Effects of the hepatitis C virus core protein on innate cellular defense pathways
    Kristen Miller
    Virology Division, Department of Laboratory Medicine, University of Washington, Seattle, WA 98104 2499, USA
    J Interferon Cytokine Res 24:391-402. 2004
    ..Core stimulated the human iNOS promoter and induced iNOS protein. The data indicate that HCV core can modulate IRF, Jak-Stat, and iNOS pathways and suggest mechanisms by which core could affect HCV persistence and pathogenesis...
  23. ncbi request reprint Hepatitis C virus-host interactions: the NS5A protein and the interferon/chemokine systems
    Khalid S A Khabar
    Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Centre, Riyadh, Kingdom of Saudi Arabia
    J Interferon Cytokine Res 22:1005-12. 2002
    ..In this review, we focus on the HCV nonstructural 5A (NS5A) protein, a model for diverse virus-host interactions, and highlight the interaction of viruses, including HCV, with the chemokine system...
  24. doi request reprint Antiviral effects of silymarin against hepatitis C: the jury is still out
    Stephen J Polyak
    Hepatology 48:345-6. 2008
  25. ncbi request reprint Associations among clinical, immunological, and viral quasispecies measurements in advanced chronic hepatitis C
    Alan Lee Rothman
    Center for Infectious Disease and Vaccine Research and Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA
    Hepatology 41:617-25. 2005
    ....
  26. pmc Mechanisms of action of interferon and ribavirin in chronic hepatitis C: Summary of a workshop
    Raymond T Chung
    Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
    Hepatology 47:306-20. 2008
  27. ncbi request reprint Hepatitis C virus NS5A colocalizes with the core protein on lipid droplets and interacts with apolipoproteins
    Stephanie T Shi
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, California 90033, USA
    Virology 292:198-210. 2002
    ....
  28. pmc Biochemical mechanism of hepatitis C virus inhibition by the broad-spectrum antiviral arbidol
    Eve Isabelle Pécheur
    IFR128 Biosciences Lyon Gerland, Institut de Biologie et Chimie des Proteines, UMR 5086 CNRS Université Claude Bernard Lyon I, 7 passage du Vercors, 69367 Lyon Cedex 07, France e pecheur ibcp fr
    Biochemistry 46:6050-9. 2007
    ..Our results suggest that the affinity of ARB for lipid membranes could account for its anti-HCV actions, together with a differential level of interaction with key motifs in HCV glycoproteins of different genotypes...
  29. ncbi request reprint Detection of hepatitis C virus RNA in normal cervical smears
    Chia Wang
    Clin Infect Dis 37:314; author reply 314-5. 2003
  30. ncbi request reprint Expressed gene clusters associated with cellular sensitivity and resistance towards anti-viral and anti-proliferative actions of interferon
    Khalid S A Khabar
    Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Center, Riyadh 11211, Saudi Arabia
    J Mol Biol 342:833-46. 2004
    ..Use of rationally designed microarray experiments in IFN biology yielded informative clusters that may provide candidate genes for diagnostic or for evaluation of therapeutic possibilities...
  31. ncbi request reprint Hepatitis C infection and alcohol use: A dangerous mix for the liver and antiviral immunity
    Gyongyi Szabo
    Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Alcohol Clin Exp Res 30:709-19. 2006
    ..Ethanol on Innate Antiviral Signaling Pathways, Hepatitis C Replication, and Human Liver Cell Transcription by Stephen Polyak; (3) Ethanol Alters Dendritic Cell Function In Vivo and Impairs the Subsequent Cellular Immune Responses to ..
  32. ncbi request reprint Engulfment of apoptotic cells expressing HCV proteins leads to differential chemokine expression and STAT signaling in human dendritic cells
    Anne M Wertheimer
    Department of Medicine, Portland VAMC and Oregon Health and Science University, Portland, OR, USA
    Hepatology 45:1422-32. 2007
    ....

Research Grants6

  1. Hepatitis C Virus Proteins and Ethanol
    STEPHEN POLYAK; Fiscal Year: 2004
    ..abstract_text> ..
  2. Hepatitis C Virus Induced IL-8 & Inhibition of Interferon
    STEPHEN POLYAK; Fiscal Year: 2007
    ..Moreover, IL-8 or its receptors may prove to be suitable targets for therapeutic intervention in chronic hepatitis C. ..