Samuel Poloyac

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. ncbi request reprint Therapeutic hypothermia-induced pharmacokinetic alterations on CYP2E1 chlorzoxazone-mediated metabolism in a cardiac arrest rat model
    Michael A Tortorici
    University of Pittsburgh School of Pharmacy, Department of Pharmaceutical Sciences, and Department of Paediatrics, the Children t Hospital of Pittsburgh, PA, USA
    Crit Care Med 34:785-91. 2006
  2. pmc Cardiac arrest and therapeutic hypothermia decrease isoform-specific cytochrome P450 drug metabolism
    Jiangquan Zhou
    Department of Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, Pittsburgh, PA 15261, USA
    Drug Metab Dispos 39:2209-18. 2011
  3. pmc Core competencies for research training in the clinical pharmaceutical sciences
    Samuel M Poloyac
    University of Pittsburgh, School of Pharmacy, Pittsburgh, PA 15261, USA
    Am J Pharm Educ 75:27. 2011
  4. ncbi request reprint The effect of isoniazid on CYP2E1- and CYP4A-mediated hydroxylation of arachidonic acid in the rat liver and kidney
    Samuel M Poloyac
    Department of Pharmaceutical Sciences, Schoolof Pharmacy, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Drug Metab Dispos 32:727-33. 2004
  5. ncbi request reprint Identification and quantification of the hydroxyeicosatetraenoic acids, 20-HETE and 12-HETE, in the cerebrospinal fluid after subarachnoid hemorrhage
    Samuel M Poloyac
    Department of Pharmaceutical Sciences, 808A Salk Hall, School of Pharmacy, University of Pittsburgh, Pittsburgh, PA 15261, USA
    J Neurosci Methods 144:257-63. 2005
  6. ncbi request reprint Protective effect of the 20-HETE inhibitor HET0016 on brain damage after temporary focal ischemia
    Samuel M Poloyac
    Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Cereb Blood Flow Metab 26:1551-61. 2006
  7. doi request reprint Lipid emulsion combined with epinephrine and vasopressin does not improve survival in a swine model of bupivacaine-induced cardiac arrest
    Shawn D Hicks
    Department of Anesthesiology, University of Ottawa, Ottawa, Ontario, Canada
    Anesthesiology 111:138-46. 2009
  8. pmc Coordinated regulation of dimethylarginine dimethylaminohydrolase-1 and cationic amino acid transporter-1 by farnesoid X receptor in mouse liver and kidney and its implication in the control of blood levels of asymmetric dimethylarginine
    Jiang Li
    Center for Pharmacogenetics, Department of Pharmaceutical Sciences, University of Pittsburgh, PA 15261, USA
    J Pharmacol Exp Ther 331:234-43. 2009
  9. ncbi request reprint Identification of omega hydroxy fatty acids in biological samples as their pentafluoropropyl derivatives by gas chromatography/mass spectrometry with positive and negative ion detection
    Michael Zemaitis
    University of Pittsburgh School of Pharmacy, Department of Pharmaceutical Sciences, 904 Salk Hall, Pittsburgh, PA 15261, USA
    Rapid Commun Mass Spectrom 16:1411-5. 2002
  10. pmc Rapid, simultaneous quantitation of mono and dioxygenated metabolites of arachidonic acid in human CSF and rat brain
    Tricia M Miller
    Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, PA 15261, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 877:3991-4000. 2009

Research Grants

  1. The Role of 20-HETE in the Pathogenesis of Stroke
    Samuel Poloyac; Fiscal Year: 2009
  2. Implications of Hypothermia on Hepatic Drug Metabolism
    Samuel Poloyac; Fiscal Year: 2007
  3. API 4000 QTRAP
    Samuel Poloyac; Fiscal Year: 2007
  4. Implications of Hypothermia on Hepatic Drug Metabolism
    Samuel Poloyac; Fiscal Year: 2009

Collaborators

Detail Information

Publications17

  1. ncbi request reprint Therapeutic hypothermia-induced pharmacokinetic alterations on CYP2E1 chlorzoxazone-mediated metabolism in a cardiac arrest rat model
    Michael A Tortorici
    University of Pittsburgh School of Pharmacy, Department of Pharmaceutical Sciences, and Department of Paediatrics, the Children t Hospital of Pittsburgh, PA, USA
    Crit Care Med 34:785-91. 2006
    ..The objective was to determine the effect of hypothermia after cardiac arrest on the in vivo kinetics of a cytochrome P450 (CYP2E1) probe drug, chlorzoxazone, and to investigate the mechanism of these alterations...
  2. pmc Cardiac arrest and therapeutic hypothermia decrease isoform-specific cytochrome P450 drug metabolism
    Jiangquan Zhou
    Department of Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, Pittsburgh, PA 15261, USA
    Drug Metab Dispos 39:2209-18. 2011
    ..05, p < 0.05, and p < 0.01, respectively). Our data indicate that hypothermia, CA, and their interaction alter cytochrome P450-isoform specific activities in an isoform-specific manner...
  3. pmc Core competencies for research training in the clinical pharmaceutical sciences
    Samuel M Poloyac
    University of Pittsburgh, School of Pharmacy, Pittsburgh, PA 15261, USA
    Am J Pharm Educ 75:27. 2011
    ..To identify and apply core competencies for training students enrolled in the clinical pharmaceutical scientist PhD training program at the University of Pittsburgh School of Pharmacy...
  4. ncbi request reprint The effect of isoniazid on CYP2E1- and CYP4A-mediated hydroxylation of arachidonic acid in the rat liver and kidney
    Samuel M Poloyac
    Department of Pharmaceutical Sciences, Schoolof Pharmacy, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Drug Metab Dispos 32:727-33. 2004
    ..It was also demonstrated that tissue concentrations parallel in vitro inhibited formation rates for 20-HETE, but not the induced 19-HETE formation in the liver...
  5. ncbi request reprint Identification and quantification of the hydroxyeicosatetraenoic acids, 20-HETE and 12-HETE, in the cerebrospinal fluid after subarachnoid hemorrhage
    Samuel M Poloyac
    Department of Pharmaceutical Sciences, 808A Salk Hall, School of Pharmacy, University of Pittsburgh, Pittsburgh, PA 15261, USA
    J Neurosci Methods 144:257-63. 2005
    ..The presence of 20-HETE in human CSF has not been demonstrated. Therefore, it was the purpose of this study to determine if HETE metabolites are present in human CSF after SAH...
  6. ncbi request reprint Protective effect of the 20-HETE inhibitor HET0016 on brain damage after temporary focal ischemia
    Samuel M Poloyac
    Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Cereb Blood Flow Metab 26:1551-61. 2006
    ..05). Brain cortical microsomal formation rate of 20-HETE was also reduced at 24 h in the ipsilateral hemisphere after MCAO. These data support a significant role for 20-HETE in the pathogenesis of ischemic stroke...
  7. doi request reprint Lipid emulsion combined with epinephrine and vasopressin does not improve survival in a swine model of bupivacaine-induced cardiac arrest
    Shawn D Hicks
    Department of Anesthesiology, University of Ottawa, Ottawa, Ontario, Canada
    Anesthesiology 111:138-46. 2009
    ..This study sought to evaluate the efficacy of lipid emulsion in reversing bupivacaine-induced cardiovascular collapse when added to a resuscitation protocol that included the use of epinephrine and vasopressin...
  8. pmc Coordinated regulation of dimethylarginine dimethylaminohydrolase-1 and cationic amino acid transporter-1 by farnesoid X receptor in mouse liver and kidney and its implication in the control of blood levels of asymmetric dimethylarginine
    Jiang Li
    Center for Pharmacogenetics, Department of Pharmaceutical Sciences, University of Pittsburgh, PA 15261, USA
    J Pharmacol Exp Ther 331:234-43. 2009
    ..These data suggest that FXR may play an important role in regulating blood levels of ADMA via coordinated regulation of DDAH-1 and CAT-1 in liver and kidney...
  9. ncbi request reprint Identification of omega hydroxy fatty acids in biological samples as their pentafluoropropyl derivatives by gas chromatography/mass spectrometry with positive and negative ion detection
    Michael Zemaitis
    University of Pittsburgh School of Pharmacy, Department of Pharmaceutical Sciences, 904 Salk Hall, Pittsburgh, PA 15261, USA
    Rapid Commun Mass Spectrom 16:1411-5. 2002
    ..The reaction appears to be specific, since derivatives of subterminal fatty acids (secondary alcohols) could not be detected...
  10. pmc Rapid, simultaneous quantitation of mono and dioxygenated metabolites of arachidonic acid in human CSF and rat brain
    Tricia M Miller
    Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, PA 15261, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 877:3991-4000. 2009
    ....
  11. pmc Endothelial nitric oxide synthase tagging single nucleotide polymorphisms and recovery from aneurysmal subarachnoid hemorrhage
    Sheila Alexander
    School of Nursing, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    Biol Res Nurs 11:42-52. 2009
    ..001) and MRS (p<.001). These data indicate that the eNOS gene plays a role in the response to SAH, which may be explained by an influence on CV...
  12. pmc Intravenous formulation of N-hydroxy-N'-(4-n-butyl-2-methylphenyl)formamidine (HET0016) for inhibition of rat brain 20-hydroxyeicosatetraenoic acid formation
    Ying Mu
    Department of Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, Pittsburgh, Pennsylvania 15261, USA
    Drug Metab Dispos 36:2324-30. 2008
    ..These results will enable future studies to determine biopharmaceutics of HET0016 for inhibition of 20-HETE after cerebral ischemia...
  13. ncbi request reprint Determination of 20-hydroxyeicosatetraenoic acid in microsomal incubates using high-performance liquid chromatography-mass spectrometry (HPLC-MS)
    Christopher A Bolcato
    Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, PA 15261, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 794:363-72. 2003
    ..5+/-39.8 pmol/min per mg; kidney K(m) and V(max): 47.6+/-8.5 microM and 1933+/-151 pM/min per mg). This paper demonstrates HPLC-MS as an efficient method for quantitating 20-HETE enzyme kinetics in microsomes from rat tissues...
  14. ncbi request reprint Effects of hypothermia on drug disposition, metabolism, and response: A focus of hypothermia-mediated alterations on the cytochrome P450 enzyme system
    Michael A Tortorici
    University of Pittsburgh School of Pharmacy, Department of Pharmaceutical Sciences, Pittsburgh, PA, USA
    Crit Care Med 35:2196-204. 2007
    ..This review examines the effects of therapeutic hypothermia on the disposition, metabolism, and response of drugs commonly used in the intensive care unit, with a focus on the cytochrome P450 enzyme system...
  15. pmc Moderate hypothermia prevents cardiac arrest-mediated suppression of drug metabolism and induction of interleukin-6 in rats
    Michael A Tortorici
    Department of Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, Pittsburgh, PA, USA
    Crit Care Med 37:263-9. 2009
    ..The objective of this study was to examine the effect of CA and hypothermia on the functional regulation of two major drug metabolizing cytochrome P450 (CYP) isoforms...
  16. pmc Cerebrospinal fluid 20-HETE is associated with delayed cerebral ischemia and poor outcomes after aneurysmal subarachnoid hemorrhage
    Elizabeth A Crago
    School of Pharmacy, University of Pittsburgh, 807 Salk Hall, Pittsburgh, PA 15261, USA
    Stroke 42:1872-7. 2011
    ..The purpose of this study was to determine whether cerebrospinal fluid 20-HETE levels were associated with DCI and long-term neuropsychological outcomes in aSAH patients...
  17. pmc Neuroglobin genetic polymorphisms and their relationship to functional outcomes after traumatic brain injury
    Pei Ying Chuang
    Acute Tertiary Care Department, Health Promotion and Development, School of Nursing, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Neurotrauma 27:999-1006. 2010
    ..Data suggest that the haplotype block represented by rs3783988 in NGB appears to influence recovery after severe TBI. Represented within this haplotype block of NGB is the region that codes for the oxygen-binding portion of NGB...

Research Grants10

  1. The Role of 20-HETE in the Pathogenesis of Stroke
    Samuel Poloyac; Fiscal Year: 2009
    ..Furthermore, these studies will provide important information about the role of monohydroxylated arachidonic acid metabolites as a contributing pathway in the pathogenesis of stroke. ..
  2. Implications of Hypothermia on Hepatic Drug Metabolism
    Samuel Poloyac; Fiscal Year: 2007
    ..Once completed, this research will provide critical information to design future clinical studies to optimize the benefits of this breakthrough therapy. ..
  3. API 4000 QTRAP
    Samuel Poloyac; Fiscal Year: 2007
    ..The acquisition of this hybrid triple quadrupole/linear ion trap mass spectrometer would provide essential instrumentation for this research and thereby remove impediments to research progress. ..
  4. Implications of Hypothermia on Hepatic Drug Metabolism
    Samuel Poloyac; Fiscal Year: 2009
    ..Once completed, this research will provide critical information to design future clinical studies to optimize the benefits of this breakthrough therapy. ..