LORRAINE A PILLUS

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc MYSTs mark chromatin for chromosomal functions
    Lorraine Pillus
    University of California, San Diego, Division of Biological Sciences, Molecular Biology and Moores Cancer Center, 9500 Gilman Drive, La Jolla, CA 92093 0347, United States
    Curr Opin Cell Biol 20:326-33. 2008
  2. pmc The SAGA subunit Ada2 functions in transcriptional silencing
    Sandra Jacobson
    Division of Biological Sciences, University of California at San Diego, 9500 Gilman Dr, 0347, San Diego, CA 92093, USA
    Mol Cell Biol 29:6033-45. 2009
  3. pmc The glucanosyltransferase Gas1 functions in transcriptional silencing
    Melissa R Koch
    Section of Molecular Biology, Division of Biological Sciences, UCSD Moores Cancer Center, University of California at San Diego, La Jolla, CA 92093 0347, USA
    Proc Natl Acad Sci U S A 106:11224-9. 2009
  4. pmc Distinct roles for the essential MYST family HAT Esa1p in transcriptional silencing
    Astrid S Clarke
    Division of Biological Sciences, UCSD Cancer Center and Center for Molecular Genetics, University of California San Diego, La Jolla, CA 92093, USA
    Mol Biol Cell 17:1744-57. 2006
  5. pmc Chromatin-modifiying enzymes are essential when the Saccharomyces cerevisiae morphogenesis checkpoint is constitutively activated
    Myriam Ruault
    Division of Biological Sciences, UCSD Moores Cancer Center, University of California, San Diego, California 92093 0347, USA
    Genetics 174:1135-49. 2006
  6. pmc Nuclear export modulates the cytoplasmic Sir2 homologue Hst2
    Jeanne M Wilson
    Division of Biological Sciences, Section of Molecular Biology, University of California, San Diego, 9500 Gilman Drive, 0347, La Jolla, California 92093, USA
    EMBO Rep 7:1247-51. 2006
  7. pmc Conserved locus-specific silencing functions of Schizosaccharomyces pombe sir2+
    Lisa L Freeman-Cook
    Division of Biological Sciences, Section of Molecular Biology and UCSD Center for Cancer Research, University of California, San Diego, 92093 0347, USA
    Genetics 169:1243-60. 2005
  8. pmc Molecular requirements for gene expression mediated by targeted histone acetyltransferases
    Sandra Jacobson
    Division of Biological Sciences, Section of Molecular Biology and Center for Molecular Genetics, UCSD Cancer Center, University of California, San Diego, La Jolla, CA 92093, USA
    Mol Cell Biol 24:6029-39. 2004
  9. ncbi request reprint Functional analyses of chromatin modifications in yeast
    Sandra J Jacobson
    Department of Biology, University of California, San Diego, La Jolla, California 92093 0347, USA
    Methods Enzymol 377:3-55. 2004
  10. pmc Slx5 promotes transcriptional silencing and is required for robust growth in the absence of Sir2
    Russell P Darst
    Department of Molecular Biology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0347, USA
    Mol Cell Biol 28:1361-72. 2008

Research Grants

Collaborators

  • Sandra N Garcia
  • Shelley L Berger
  • SUSAN FORSBURG
  • JAMES KADONAGA
  • Trey Ideker
  • R Marmorstein
  • Eileen J Kennedy
  • Sandra Jacobson
  • Melissa R Koch
  • Erin M Scott
  • Christie S Chang
  • H Craig Mak
  • Russell P Darst
  • Gourisankar Ghosh
  • Astrid S Clarke
  • Myriam Ruault
  • Jeanne M Wilson
  • Lisa L Freeman-Cook
  • Wan Sheng Lo
  • Sandra J Jacobson
  • Xuejun Huang Parsons
  • Collin Zimmerman
  • Eva Samal
  • Viet Q Le
  • John Marlett
  • David Yang
  • Eric R Gamache
  • Eliana B G√≥mez
  • Erik J Spedale
  • Karl W Henry
  • Patricia Laurenson
  • Patricia M Laurenson

Detail Information

Publications17

  1. pmc MYSTs mark chromatin for chromosomal functions
    Lorraine Pillus
    University of California, San Diego, Division of Biological Sciences, Molecular Biology and Moores Cancer Center, 9500 Gilman Drive, La Jolla, CA 92093 0347, United States
    Curr Opin Cell Biol 20:326-33. 2008
    ..Z, and remodeling complexes to demarcate silent and active chromosomal domains, facilitate transcription, and enable repair of DNA damage...
  2. pmc The SAGA subunit Ada2 functions in transcriptional silencing
    Sandra Jacobson
    Division of Biological Sciences, University of California at San Diego, 9500 Gilman Dr, 0347, San Diego, CA 92093, USA
    Mol Cell Biol 29:6033-45. 2009
    ..Thus, Ada2, likely in the context of SAGA, is positioned at chromosomal termini to participate in both transcriptional repression and activation in response to nutrient signaling...
  3. pmc The glucanosyltransferase Gas1 functions in transcriptional silencing
    Melissa R Koch
    Section of Molecular Biology, Division of Biological Sciences, UCSD Moores Cancer Center, University of California at San Diego, La Jolla, CA 92093 0347, USA
    Proc Natl Acad Sci U S A 106:11224-9. 2009
    ....
  4. pmc Distinct roles for the essential MYST family HAT Esa1p in transcriptional silencing
    Astrid S Clarke
    Division of Biological Sciences, UCSD Cancer Center and Center for Molecular Genetics, University of California San Diego, La Jolla, CA 92093, USA
    Mol Biol Cell 17:1744-57. 2006
    ....
  5. pmc Chromatin-modifiying enzymes are essential when the Saccharomyces cerevisiae morphogenesis checkpoint is constitutively activated
    Myriam Ruault
    Division of Biological Sciences, UCSD Moores Cancer Center, University of California, San Diego, California 92093 0347, USA
    Genetics 174:1135-49. 2006
    ..A catalytically dead Hsl7p retained wild-type interactions, implying that modification of histone H3 or H4 N termini by Gcn5p, Esa1p, Rpd3p, and Set1p, but not by Hsl7p, was needed to bypass the morphogenesis checkpoint...
  6. pmc Nuclear export modulates the cytoplasmic Sir2 homologue Hst2
    Jeanne M Wilson
    Division of Biological Sciences, Section of Molecular Biology, University of California, San Diego, 9500 Gilman Drive, 0347, La Jolla, California 92093, USA
    EMBO Rep 7:1247-51. 2006
    ..Our identification of putative nuclear export sequences in numerous vertebrate SIRT2 proteins shows that active nuclear export can be a conserved mechanism for regulating Sir2 homologues...
  7. pmc Conserved locus-specific silencing functions of Schizosaccharomyces pombe sir2+
    Lisa L Freeman-Cook
    Division of Biological Sciences, Section of Molecular Biology and UCSD Center for Cancer Research, University of California, San Diego, 92093 0347, USA
    Genetics 169:1243-60. 2005
    ..These results also suggest that, despite differences in most of the other molecules required, the two distantly related yeast species share a mechanism for targeting Sir2p homologs to silent chromatin...
  8. pmc Molecular requirements for gene expression mediated by targeted histone acetyltransferases
    Sandra Jacobson
    Division of Biological Sciences, Section of Molecular Biology and Center for Molecular Genetics, UCSD Cancer Center, University of California, San Diego, La Jolla, CA 92093, USA
    Mol Cell Biol 24:6029-39. 2004
    ..The telomeric location of the reporter gene used in these studies also provides insight into the molecular requirements for heterochromatin boundary formation and for overcoming transcriptional silencing...
  9. ncbi request reprint Functional analyses of chromatin modifications in yeast
    Sandra J Jacobson
    Department of Biology, University of California, San Diego, La Jolla, California 92093 0347, USA
    Methods Enzymol 377:3-55. 2004
  10. pmc Slx5 promotes transcriptional silencing and is required for robust growth in the absence of Sir2
    Russell P Darst
    Department of Molecular Biology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0347, USA
    Mol Cell Biol 28:1361-72. 2008
    ..These results indicate that Sir2 and Slx5 jointly contribute to transcriptional silencing and robust cellular growth...
  11. pmc Dynamic reprogramming of transcription factors to and from the subtelomere
    H Craig Mak
    Division of Biological Sciences, University of California San Diego, La Jolla, California 92093, USA
    Genome Res 19:1014-25. 2009
    ..Taken together, these results underscore the importance of genome structure in understanding the regulatory dynamics of transcriptional networks...
  12. pmc Homocitrate synthase connects amino acid metabolism to chromatin functions through Esa1 and DNA damage
    Erin M Scott
    Division of Biological Sciences, Molecular Biology, University of California at San Diego, La Jolla, California 92093, USA
    Genes Dev 24:1903-13. 2010
    ..Thus, Lys20 appears to have evolved as a bifunctional protein that connects cellular metabolism with chromatin functions...
  13. pmc Histone H3 phosphorylation can promote TBP recruitment through distinct promoter-specific mechanisms
    Wan Sheng Lo
    Division of Biological Sciences and UCSD Cancer Center, University of California, San Diego, CA, USA
    EMBO J 24:997-1008. 2005
    ..Our results emphasize that histone modifications share general functions between promoters, but also acquire distinct roles tailored for promoter-specific requirements...
  14. ncbi request reprint Identification of functionally distinct regions that mediate biological activity of the protein kinase a homolog Tpk2
    Eileen J Kennedy
    Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093 0375, USA
    J Biol Chem 283:1084-93. 2008
    ..Because many of these map outside the conserved core, this approach should be broadly useful in identifying new, more kinase-specific therapeutic targets...
  15. pmc Collaboration between the essential Esa1 acetyltransferase and the Rpd3 deacetylase is mediated by H4K12 histone acetylation in Saccharomyces cerevisiae
    Christie S Chang
    Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego UCSD Moores Cancer Center, La Jolla, California 92093 0347, USA
    Genetics 183:149-60. 2009
    ....
  16. pmc Histone deacetylation by Sir2 generates a transcriptionally repressed nucleoprotein complex
    Xuejun Huang Parsons
    Section of Molecular Biology, 0347, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0347, USA
    Proc Natl Acad Sci U S A 100:1609-14. 2003
    ..These findings suggest that deacetylation by Sir2 causes a conformational change or rearrangement of histones into a transcriptionally repressive chromatin structure...
  17. pmc Pho5p and newly identified nucleotide pyrophosphatases/ phosphodiesterases regulate extracellular nucleotide phosphate metabolism in Saccharomyces cerevisiae
    Eileen J Kennedy
    Department of Chemistry and Biochemistry, and UCSD Cancer Center, University of California, San Diego, La Jolla, CA 92093 0347, USA
    Eukaryot Cell 4:1892-901. 2005
    ..This study demonstrates that extracellular nucleotide phosphate metabolism appears to be controlled by at least two independent regulatory mechanisms, uniting phosphate starvation with extracellular nucleotide regulation...

Research Grants20

  1. Functional Analysis of the SIR2/HST Deacetylases
    Lorraine Pillus; Fiscal Year: 2005
    ..These aims will be accomplished through a combination of molecular genetic, cell biological and biochemical approaches that will be extended with emerging microarray technologies. ..
  2. Analysis of MYST Family Acetyltransferase Functions
    Lorraine Pillus; Fiscal Year: 2007
    ..Proteomic approaches will identify non-histone substrates, the significance of which will be evaluated through combined biochemical and genetic approaches. ..
  3. SAS GENE FUNCTIONS AND CHROMATIN AND SILENCING
    Lorraine Pillus; Fiscal Year: 2001
    ..Results from these diverse experimental approaches should establish mechanisms of SAS gene function and suggest how disruption of this function leads to alterations in genomic silencing and activation. ..
  4. FUNCTIONAL ANALYSIS OF THE SIR2 SILENCING PROTEIN
    Lorraine Pillus; Fiscal Year: 2001
    ..Progress made understanding these centrally important regulatory mechanisms may ultimately lead to improved diagnostics and refined therapeutic and genetic interventions for human diseases resulting from loss of gene regulation. ..
  5. Chromatin Regulation by Deacetylation and SUMO-Targeted Ubiquitin Ligation
    Lorraine Pillus; Fiscal Year: 2010
    ..Understanding their cellular roles, substrates and regulation will ultimately provide insight into basic processes that define human development and disease, and may be particularly relevant to mechanisms of healthy aging and cancer. ..