G J Pielak

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. pmc An upper limit for macromolecular crowding effects
    Andrew C Miklos
    Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    BMC Biophys 4:13. 2011
  2. pmc Protein nuclear magnetic resonance under physiological conditions
    Gary J Pielak
    Department of Chemistry, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Biochemistry 48:226-34. 2009
  3. pmc Woes of proline: a cautionary kinetic tale
    Gary J Pielak
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Protein Sci 15:393-4. 2006
  4. pmc A model of intracellular organization
    Gary J Pielak
    Department of Chemistry, Program in Molecular Biology and Biotechnology, and the Lineberger Cancer Research Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 102:5901-2. 2005
  5. ncbi request reprint Interactions between yeast iso-1-cytochrome c and its peroxidase
    G J Pielak
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3290, USA
    Biochemistry 40:422-8. 2001
  6. ncbi request reprint Effects of crowding by mono-, di-, and tetrasaccharides on cytochrome c-cytochrome c peroxidase binding: comparing experiment to theory
    A S Morar
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3290, USA
    Biochemistry 40:281-5. 2001
  7. ncbi request reprint Testing hypotheses about determinants of protein structure with high-precision, high-throughput stability measurements and statistical modeling
    Fang Yi
    Departments of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Biochemistry 42:7594-603. 2003
  8. ncbi request reprint Characterization of horse cytochrome c expressed in Escherichia coli
    C N Patel
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Protein Expr Purif 22:220-4. 2001
  9. ncbi request reprint High-precision, high-throughput stability determinations facilitated by robotics and a semiautomated titrating fluorometer
    Marshall Hall Edgell
    Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Biochemistry 42:7587-93. 2003
  10. pmc Second virial coefficients as a measure of protein--osmolyte interactions
    G T Weatherly
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Protein Sci 10:12-6. 2001

Collaborators

  • Conggang Li
  • X Wang
  • N L Thompson
  • JULIETTE LECOMTE
  • Julie E Bryant
  • D J Winzor
  • Mohanish Deshmukh
  • FRANCIS S contact MILLETT
  • Lisa M Charlton
  • Andrew C Miklos
  • Gregory B Young
  • Kristin M Slade
  • Brian C McNulty
  • Chetan N Patel
  • Naima G Sharaf
  • A S Morar
  • Ashutosh Tripathy
  • Yaqiang Wang
  • Alina Olteanu
  • Fang Yi
  • Marshall Hall Edgell
  • Christopher O Barnes
  • Matthew M Dedmon
  • Rebecca A S Ruf
  • Jillian Orans
  • Shannon J McCall
  • Aleister J Saunders
  • Joseph D Batchelor
  • Dorothy A Sims
  • Gregory Engstrom
  • Artemiza S Morar
  • James R Beasley
  • C N Patel
  • G T Weatherly
  • P R Davis-Searles
  • L Andrew Lyon
  • Courtney D Sorrell
  • Michael Chua
  • Bridgett L Steele
  • Rachael Baker
  • Imola G Zigoneanu
  • Evan A Lutz
  • Rashid Nassar
  • Page A W Anderson
  • Kenneth A Alexander
  • Paul M Henderson
  • Nadia N Malouf
  • R John Solaro
  • Annette E Oakeley
  • Scott Kennedy
  • Sany Rajagukguk
  • Patrick R Potts
  • Bernard Trumpower
  • Chang An Yu
  • Bill Durham
  • Ray Rajagukguk
  • Kunhong Xiao
  • Linda Yu
  • Camille M Minder
  • Torsten Merbitz-Zahradnik
  • Michael W Linhoff
  • Schroeder M Noble
  • Bryan R Fine
  • Gresham T Weatherly
  • Lixin Chen
  • Donald F Doyle
  • David S Cohen
  • M C Lind
  • A Olteanu
  • A J Saunders
  • D A Erie
  • G B Young

Detail Information

Publications38

  1. pmc An upper limit for macromolecular crowding effects
    Andrew C Miklos
    Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    BMC Biophys 4:13. 2011
    ..abstract:..
  2. pmc Protein nuclear magnetic resonance under physiological conditions
    Gary J Pielak
    Department of Chemistry, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Biochemistry 48:226-34. 2009
    ....
  3. pmc Woes of proline: a cautionary kinetic tale
    Gary J Pielak
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Protein Sci 15:393-4. 2006
  4. pmc A model of intracellular organization
    Gary J Pielak
    Department of Chemistry, Program in Molecular Biology and Biotechnology, and the Lineberger Cancer Research Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 102:5901-2. 2005
  5. ncbi request reprint Interactions between yeast iso-1-cytochrome c and its peroxidase
    G J Pielak
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3290, USA
    Biochemistry 40:422-8. 2001
    ..Comparing these energies to the crystal structure of the complex leads to the conclusion that many of the substitutions induce a rearrangement of the complex...
  6. ncbi request reprint Effects of crowding by mono-, di-, and tetrasaccharides on cytochrome c-cytochrome c peroxidase binding: comparing experiment to theory
    A S Morar
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3290, USA
    Biochemistry 40:281-5. 2001
    ..None of the analyses are completely successful by themselves, and the results suggest that a complete analysis must account for both excluded-volume and chemical interactions...
  7. ncbi request reprint Testing hypotheses about determinants of protein structure with high-precision, high-throughput stability measurements and statistical modeling
    Fang Yi
    Departments of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Biochemistry 42:7594-603. 2003
    ..We see no correlation between the residuals derived from the full model and m(mut) - m(wt), and hence it is unlikely our m(mut) values reflect mutant-to-mutant differences in the denatured state...
  8. ncbi request reprint Characterization of horse cytochrome c expressed in Escherichia coli
    C N Patel
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Protein Expr Purif 22:220-4. 2001
    ..The expression system will help advance our understanding of the roles of cytochrome c in electron transfer, oxidative stress, and apoptosis by allowing the production of protein variants...
  9. ncbi request reprint High-precision, high-throughput stability determinations facilitated by robotics and a semiautomated titrating fluorometer
    Marshall Hall Edgell
    Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Biochemistry 42:7587-93. 2003
    ....
  10. pmc Second virial coefficients as a measure of protein--osmolyte interactions
    G T Weatherly
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Protein Sci 10:12-6. 2001
    ..We have interpreted the second virial coefficient as a measure of both excluded volume and protein--osmolyte binding. We conclude that simple models are not sufficient to understand the interactions between osmolytes and proteins...
  11. ncbi request reprint Interpreting the effects of small uncharged solutes on protein-folding equilibria
    P R Davis-Searles
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Annu Rev Biophys Biomol Struct 30:271-306. 2001
    ..This phenomenon clearly has potential ramifications in the cell, where the crowded environment could well induce the same effects...
  12. ncbi request reprint Crowding by trisaccharides and the 2:1 cytochrome c-cytochrome c peroxidase complex
    Artemiza S Morar
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3290, USA
    Biochemistry 41:547-51. 2002
    ..Mutagenesis studies confirm that the second site includes Asp 148 on the peroxidase. Binding of both cytochrome c molecules is exothermic. The data are interpreted by assuming either the presence or absence of intersite interactions...
  13. pmc Protein (19)F NMR in Escherichia coli
    Conggang Li
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Am Chem Soc 132:321-7. 2010
    ..We also show that site-specific structural and dynamic information about both globular and disordered proteins can be obtained inside cells by using (19)F NMR...
  14. pmc Residue-level interrogation of macromolecular crowding effects on protein stability
    Lisa M Charlton
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Am Chem Soc 130:6826-30. 2008
    ..Our data reinforce the assertion that macromolecular crowding stabilizes the protein by destabilizing its unfolded states...
  15. ncbi request reprint Stability and apoptotic activity of recombinant human cytochrome c
    Alina Olteanu
    Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599, USA
    Biochem Biophys Res Commun 312:733-40. 2003
    ..We use data from this assay along with data from the literature to define the apaf-1 binding site on human cytochrome c...
  16. pmc Solvent-induced collapse of alpha-synuclein and acid-denatured cytochrome c
    A S Morar
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Protein Sci 10:2195-9. 2001
    ..Taken together, these data show that alpha-synuclein, a natively unfolded protein, is collapsed even in dilute solution, but lacks structure...
  17. pmc Alpha-Synuclein conformation affects its tyrosine-dependent oxidative aggregation
    Rebecca A S Ruf
    Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Biochemistry 47:13604-9. 2008
    ..We also show that preformed oxidative aggregates are not incorporated into noncovalent fibrils. These data provide insight into how dityrosine may be formed in Lewy bodies seen in Parkinson's disease...
  18. ncbi request reprint Searching for quantitative entropy-enthalpy compensation among protein variants
    James R Beasley
    Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599 3290, USA
    Proteins 49:398-402. 2002
    ..This study reinforces the idea that DeltaH-versus-DeltaS plots should not be used to provide evidence for SH compensation...
  19. pmc A bioreactor for in-cell protein NMR
    Naima G Sharaf
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Magn Reson 202:140-6. 2010
    ..We have utilized the bioreactor to study the expression of the natively disordered protein alpha-synuclein, inside Escherichia coli cells...
  20. pmc Translational and rotational diffusion of a small globular protein under crowded conditions
    Conggang Li
    Department of Chemistry, Department of Biochemistry and Biophysics, and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Phys Chem B 113:13390-2. 2009
    ..We discuss our results in terms of other studies on the effects of macromolecules on globular protein diffusion...
  21. ncbi request reprint Cytosol has a small effect on protein backbone dynamics
    Julie E Bryant
    Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Biochemistry 45:10085-91. 2006
    ..Increases in the time scale of both the picosecond and millisecond motions are observed, but the increases are less than approximately 30%...
  22. pmc Quantifying green fluorescent protein diffusion in Escherichia coli by using continuous photobleaching with evanescent illumination
    Kristin M Slade
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 3290, USA
    J Phys Chem B 113:4837-45. 2009
    ..We present the theoretical basis for the technique and demonstrate its applicability by measuring the diffusion coefficient, 6.3 +/- 1.1 microm(2)/s, of green fluorescent protein in Escherichia coli cells...
  23. doi request reprint Effects of recombinant protein expression on green fluorescent protein diffusion in Escherichia coli
    Kristin M Slade
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3290, USA
    Biochemistry 48:5083-9. 2009
    ..We conclude that expression of these soluble proteins has little to no effect on the diffusion of GFP. These results have implications for the utility of in-cell nuclear magnetic resonance spectroscopy...
  24. pmc Temperature-induced reversible conformational change in the first 100 residues of alpha-synuclein
    Brian C McNulty
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Protein Sci 15:602-8. 2006
    ..The properties of globular proteins change little with solution conditions until they denature cooperatively, but the properties of natively disordered proteins can vary dramatically with solution conditions...
  25. ncbi request reprint Macromolecular crowding in the Escherichia coli periplasm maintains alpha-synuclein disorder
    Brian C McNulty
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Mol Biol 355:893-7. 2006
    ....
  26. ncbi request reprint Protein dynamics in living cells
    Julie E Bryant
    Departments of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Biochemistry 44:9275-9. 2005
    ..Therefore, it appears that dilute solution steady-state {1H}-15N nOe measurements provide biologically relevant information about pico- to nanosecond backbone motion in proteins...
  27. ncbi request reprint Impact of protein denaturants and stabilizers on water structure
    Joseph D Batchelor
    Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Am Chem Soc 126:1958-61. 2004
    ..Our results indicate that efforts to explain solute effects should focus on other hypotheses, including those based on preferential interaction and excluded volume...
  28. pmc Effects of proteins on protein diffusion
    Yaqiang Wang
    Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Am Chem Soc 132:9392-7. 2010
    ..In summary, weak but nonspecific, noncovalent chemical interactions between proteins appear to fundamentally impact protein diffusion in cells...
  29. pmc 19F NMR studies of alpha-synuclein conformation and fibrillation
    Conggang Li
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Biochemistry 48:8578-84. 2009
    ..We conclude that 19F NMR spectroscopy is useful for obtaining residue-level, quantitative information about the structure, binding, and aggregation of alpha-synuclein...
  30. pmc Volume exclusion and soft interaction effects on protein stability under crowded conditions
    Andrew C Miklos
    Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Biochemistry 49:6984-91. 2010
    ..We conclude that the role of native-state binding and other soft interactions needs to be seriously considered when applying both theory and experiment to studies of macromolecular crowding...
  31. pmc FlgM gains structure in living cells
    Matthew M Dedmon
    Departments of Chemistry, and Biochemistry and Biophysics, and Lineberger Cancer Research Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 99:12681-4. 2002
    ....
  32. pmc Using NMR to distinguish viscosity effects from nonspecific protein binding under crowded conditions
    Conggang Li
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Am Chem Soc 131:1368-9. 2009
    ..We describe a simple and reliable NMR method to distinguish viscosity effects from binding and aggregation under crowded conditions...
  33. pmc Differential dynamical effects of macromolecular crowding on an intrinsically disordered protein and a globular protein: implications for in-cell NMR spectroscopy
    Conggang Li
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Am Chem Soc 130:6310-1. 2008
    ..From in vitro studies we conclude that this difference in detectability is the result of the differential dynamical response of disordered and ordered proteins to the changes of motion caused by the increased viscosity in cells...
  34. pmc Peeking into living eukaryotic cells with high-resolution NMR
    Lisa M Charlton
    Department of Chemistry and Biochemistry, Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 103:11817-8. 2006
  35. pmc Effects of molecular crowding by saccharides on alpha-chymotrypsin dimerization
    Chetan N Patel
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Protein Sci 11:997-1003. 2002
    ....
  36. ncbi request reprint Development and cardiac contractility: cardiac troponin T isoforms and cytosolic calcium in rabbit
    Shannon J McCall
    Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA
    Pediatr Res 60:276-81. 2006
    ..2 +/- 1.6 pA/pF). The higher calcium sensitivity of Tn-Ca binding and of force development conferred by rcTnT(1) suggest that higher neonatal cTnT(1) expression may partially compensate for the lower systolic [Ca(2+)](i)...
  37. ncbi request reprint Design of a ruthenium-labeled cytochrome c derivative to study electron transfer with the cytochrome bc1 complex
    Gregory Engstrom
    Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas 72701, USA
    Biochemistry 42:2816-24. 2003
    ..The interaction of yeast Ru-39-Cc with yeast cytochrome bc1 is stronger than that of horse Ru-39-Cc with bovine cytochrome bc1, suggesting that nonpolar interactions are stronger in the yeast system...
  38. ncbi request reprint Reconsideration of sedimentation equilibrium distributions reflecting the effects of small inert cosolutes on the dimerization of alpha-chymotrypsin
    Donald J Winzor
    Department of Biochemistry, University of Queensland, Brisbane, Queensland 4072, Australia
    Biophys Chem 130:89-92. 2007
    ..Support is thereby provided for the earlier contention that the effect of sucrose, as well as of glucose and raffinose, on dimerization may be rationalized quantitatively in terms of molecular crowding by an inert cosolute...