Raymond Pickles

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. doi request reprint Human airway epithelial cell cultures for modeling respiratory syncytial virus infection
    Raymond J Pickles
    Department of Microbiology and Immunology, UNC School of Medicine, 7021 Thurston Bowles, Campus Box 7248, Chapel Hill, NC, 27599 7248, USA
    Curr Top Microbiol Immunol 372:371-87. 2013
  2. pmc Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense
    Mehmet Kesimer
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    FASEB J 23:1858-68. 2009
  3. pmc Tracheobronchial air-liquid interface cell culture: a model for innate mucosal defense of the upper airways?
    Mehmet Kesimer
    Dept of Biochemistry and Biophysics, Univ of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Am J Physiol Lung Cell Mol Physiol 296:L92-L100. 2009
  4. pmc SARS-CoV replication and pathogenesis in an in vitro model of the human conducting airway epithelium
    Amy C Sims
    Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States
    Virus Res 133:33-44. 2008
  5. pmc Respiratory syncytial virus infection of human airway epithelial cells is polarized, specific to ciliated cells, and without obvious cytopathology
    Liqun Zhang
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, North Carolina 27599 7248, USA
    J Virol 76:5654-66. 2002
  6. pmc Severe acute respiratory syndrome coronavirus infection of human ciliated airway epithelia: role of ciliated cells in viral spread in the conducting airways of the lungs
    Amy C Sims
    Department of Epidemiology, University of North Carolina at Chapel Hill, 2107 McGavran Greenberg Hall, CB 7435, Chapel Hill, NC 27599 7435, USA
    J Virol 79:15511-24. 2005
  7. ncbi request reprint Physical and biological barriers to viral vector-mediated delivery of genes to the airway epithelium
    Raymond J Pickles
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7248, USA
    Proc Am Thorac Soc 1:302-8. 2004
  8. pmc Normal and cystic fibrosis airway surface liquid homeostasis. The effects of phasic shear stress and viral infections
    Robert Tarran
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7248, USA
    J Biol Chem 280:35751-9. 2005
  9. ncbi request reprint The effect of respiratory synctial virus on chemokine release by differentiated airway epithelium
    Thomas E Mellow
    Department of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7220, USA
    Exp Lung Res 30:43-57. 2004
  10. pmc Glycocalyx restricts adenoviral vector access to apical receptors expressed on respiratory epithelium in vitro and in vivo: role for tethered mucins as barriers to lumenal infection
    Jaclyn R Stonebraker
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, 7021 Thurston Bowles, Chapel Hill, NC 27759 7248, USA
    J Virol 78:13755-68. 2004

Research Grants

Collaborators

Detail Information

Publications17

  1. doi request reprint Human airway epithelial cell cultures for modeling respiratory syncytial virus infection
    Raymond J Pickles
    Department of Microbiology and Immunology, UNC School of Medicine, 7021 Thurston Bowles, Campus Box 7248, Chapel Hill, NC, 27599 7248, USA
    Curr Top Microbiol Immunol 372:371-87. 2013
    ....
  2. pmc Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense
    Mehmet Kesimer
    Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    FASEB J 23:1858-68. 2009
    ..Taken together, these findings suggest that airway epithelial cells release exosome-like vesicles and that these structures may be involved in diverse physiological processes in airway biology, including innate mucosal defense...
  3. pmc Tracheobronchial air-liquid interface cell culture: a model for innate mucosal defense of the upper airways?
    Mehmet Kesimer
    Dept of Biochemistry and Biophysics, Univ of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Am J Physiol Lung Cell Mol Physiol 296:L92-L100. 2009
    ..This shows that the primary cell culture system is an important model for study of aspects of innate defense of the upper airways related specifically to mucus consisting solely of airway cell products...
  4. pmc SARS-CoV replication and pathogenesis in an in vitro model of the human conducting airway epithelium
    Amy C Sims
    Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States
    Virus Res 133:33-44. 2008
    ..Efficient SARS-CoV infection of ciliated cell-types in HAE provides a useful in vitro model of human lung origin to study characteristics of SARS-CoV replication and pathogenesis...
  5. pmc Respiratory syncytial virus infection of human airway epithelial cells is polarized, specific to ciliated cells, and without obvious cytopathology
    Liqun Zhang
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, North Carolina 27599 7248, USA
    J Virol 76:5654-66. 2002
    ..Since rgRSV appears to breach the lumenal barriers encountered by other gene transfer vectors in the airway, this virus may be a good candidate for the development of a gene transfer vector for CF lung disease...
  6. pmc Severe acute respiratory syndrome coronavirus infection of human ciliated airway epithelia: role of ciliated cells in viral spread in the conducting airways of the lungs
    Amy C Sims
    Department of Epidemiology, University of North Carolina at Chapel Hill, 2107 McGavran Greenberg Hall, CB 7435, Chapel Hill, NC 27599 7435, USA
    J Virol 79:15511-24. 2005
    ..Efficient SARS-CoV infection of ciliated cells in HAE provides a useful in vitro model of human lung origin to study characteristics of SARS-CoV replication and pathogenesis...
  7. ncbi request reprint Physical and biological barriers to viral vector-mediated delivery of genes to the airway epithelium
    Raymond J Pickles
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7248, USA
    Proc Am Thorac Soc 1:302-8. 2004
    ..Alternative viral vectors that have evolved to circumvent these barriers will require further development if gene transfer is ever to be considered a therapy for CF lung disease...
  8. pmc Normal and cystic fibrosis airway surface liquid homeostasis. The effects of phasic shear stress and viral infections
    Robert Tarran
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7248, USA
    J Biol Chem 280:35751-9. 2005
    ....
  9. ncbi request reprint The effect of respiratory synctial virus on chemokine release by differentiated airway epithelium
    Thomas E Mellow
    Department of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7220, USA
    Exp Lung Res 30:43-57. 2004
    ..In epithelial layers, virus-containing cells are the predominant source of the increased chemokine release. The authors speculate that similar processes in vivo influence recruitment of leukocytes to sites of RSV infection...
  10. pmc Glycocalyx restricts adenoviral vector access to apical receptors expressed on respiratory epithelium in vitro and in vivo: role for tethered mucins as barriers to lumenal infection
    Jaclyn R Stonebraker
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, 7021 Thurston Bowles, Chapel Hill, NC 27759 7248, USA
    J Virol 78:13755-68. 2004
    ....
  11. pmc Human parainfluenza virus type 1 C proteins are nonessential proteins that inhibit the host interferon and apoptotic responses and are required for efficient replication in nonhuman primates
    Emmalene J Bartlett
    Laboratory of Infectious Diseases, Respiratory Viruses Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, U S Department of Health and Human Services, Bethesda, Maryland 20892 8007, USA
    J Virol 82:8965-77. 2008
    ..Thus, the C proteins of HPIV1 are nonessential but have anti-IFN and antiapoptosis activities required for virulence in primates...
  12. pmc RSV604, a novel inhibitor of respiratory syncytial virus replication
    Joanna Chapman
    Arrow Therapeutics Ltd, Britannia House, 7 Trinity Street, London SE1 1DB, United Kingdom
    Antimicrob Agents Chemother 51:3346-53. 2007
    ..RSV604, which is currently in phase II clinical trials, represents the first in a new class of RSV inhibitors and may have significant potential for the effective treatment of RSV disease...
  13. ncbi request reprint SARS CoV replication and pathogenesis in human airway epithelial cultures
    Amy C Sims
    University of North Carolina at Chapel Hill, 27599, USA
    Adv Exp Med Biol 581:535-8. 2006
  14. pmc Infection of ciliated cells by human parainfluenza virus type 3 in an in vitro model of human airway epithelium
    Liqun Zhang
    CF Pulmonary Research and Treatment Center, 7021 Thurston Bowles, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7248, USA
    J Virol 79:1113-24. 2005
    ..HAE derived from cystic fibrosis patients was not more susceptible to rgPIV3 infection but did exhibit limited spread of virus due to impaired movement of lumenal secretions due to compromised function of the cilia...
  15. pmc Francisella tularensis replicates within alveolar type II epithelial cells in vitro and in vivo following inhalation
    Joshua D Hall
    University of North Carolina, Chapel Hill, NC 27599 7290, USA
    Infect Immun 75:1034-9. 2007
    ..F. tularensis LVS invaded and replicated within alveolar epithelial cell lines. Following intranasal inoculation of C57BL/6 mice, Francisella localized to the alveolus and replicated within alveolar type II epithelial cells...
  16. ncbi request reprint Cytokine amplification by respiratory syncytial virus infection in human nasal epithelial cells
    Subinoy Das
    Department of Otolaryngology Head and Neck Surgery, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Laryngoscope 115:764-8. 2005
    ....
  17. pmc Infection of human airway epithelium by human and avian strains of influenza a virus
    Catherine I Thompson
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27759 7248, USA
    J Virol 80:8060-8. 2006
    ....

Research Grants7

  1. Viral-Bacterial Interactions in the Airway Epithelium
    Raymond Pickles; Fiscal Year: 2006
    ....
  2. CFTR Delivery to Ciliated Airway Cells by PIV Vectors
    Raymond Pickles; Fiscal Year: 2009
    ....