KEVIN PFISTER

Summary

Affiliation: University of Virginia
Country: USA

Publications

  1. ncbi request reprint Identification and molecular characterization of the p24 dynactin light chain
    K K Pfister
    Department of Cell Biology, University of Virginia Health Science Center, Charlottesville 22908 0439, USA
    Cell Motil Cytoskeleton 41:154-67. 1998
  2. pmc Cytoplasmic dynein nomenclature
    K Kevin Pfister
    Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    J Cell Biol 171:411-3. 2005
  3. pmc Genetic analysis of the cytoplasmic dynein subunit families
    K Kevin Pfister
    Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville, Virginia, USA
    PLoS Genet 2:e1. 2006
  4. ncbi request reprint The roadblock light chain binds a novel region of the cytoplasmic Dynein intermediate chain
    Stephen J Susalka
    Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    J Biol Chem 277:32939-46. 2002
  5. ncbi request reprint The DYNLT3 light chain directly links cytoplasmic dynein to a spindle checkpoint protein, Bub3
    Kevin W H Lo
    Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville, Virginia 22908 0732, USA
    J Biol Chem 282:11205-12. 2007
  6. ncbi request reprint Methods to study the interactions of the dynein light chains and intermediate chains
    Kevin W H Lo
    Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA, USA
    Methods Mol Biol 392:85-95. 2007
  7. pmc A neuron-specific cytoplasmic dynein isoform preferentially transports TrkB signaling endosomes
    Junghoon Ha
    Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville, VA 22908, USA
    J Cell Biol 181:1027-39. 2008
  8. ncbi request reprint Intermediate chain subunit as a probe for cytoplasmic dynein function: biochemical analyses and live cell imaging in PC12 cells
    Kenneth R Myers
    Cell Biology Department, School of Medicine, University of Virginia, Charlottesville, Virginia, USA
    J Neurosci Res 85:2640-7. 2007
  9. ncbi request reprint Identification of a novel region of the cytoplasmic Dynein intermediate chain important for dimerization in the absence of the light chains
    Kevin W H Lo
    Department of Cell Biology, University of Virginia, Charlottesville, Virginia 22908, USA
    J Biol Chem 281:9552-9. 2006
  10. ncbi request reprint Interaction of the DYNLT (TCTEX1/RP3) light chains and the intermediate chains reveals novel intersubunit regulation during assembly of the dynein complex
    Kevin W H Lo
    Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville, Virginia 22908, USA
    J Biol Chem 282:36871-8. 2007

Research Grants

  1. The Role of Cytoplasmic Dynein in Axonal Transport
    KEVIN K PFISTER; Fiscal Year: 2010
  2. The Role of Cytoplasmic Dynein in Axonal Transport
    KEVIN PFISTER; Fiscal Year: 2009
  3. The Role of Cytoplasmic Dynein in Axonal Transport
    KEVIN PFISTER; Fiscal Year: 2006
  4. ORGANELLE MOVEMENT IN FAST AXONAL TRANSPORT
    KEVIN PFISTER; Fiscal Year: 2001
  5. The Role of Cytoplasmic Dynein in Axonal Transport
    KEVIN PFISTER; Fiscal Year: 2009

Collaborators

Detail Information

Publications14

  1. ncbi request reprint Identification and molecular characterization of the p24 dynactin light chain
    K K Pfister
    Department of Cell Biology, University of Virginia Health Science Center, Charlottesville 22908 0439, USA
    Cell Motil Cytoskeleton 41:154-67. 1998
    ....
  2. pmc Cytoplasmic dynein nomenclature
    K Kevin Pfister
    Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    J Cell Biol 171:411-3. 2005
    ..This nomenclature recognizes the two distinct cytoplasmic dynein complexes and has the flexibility to accommodate the discovery of new subunits and isoforms...
  3. pmc Genetic analysis of the cytoplasmic dynein subunit families
    K Kevin Pfister
    Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville, Virginia, USA
    PLoS Genet 2:e1. 2006
    ....
  4. ncbi request reprint The roadblock light chain binds a novel region of the cytoplasmic Dynein intermediate chain
    Stephen J Susalka
    Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    J Biol Chem 277:32939-46. 2002
    ..In addition, this roadblock-binding domain is significantly downstream from both the Tctex1- and LC8-binding sites, supporting the hypothesis that multiple light chain family members can bind to the same intermediate chain...
  5. ncbi request reprint The DYNLT3 light chain directly links cytoplasmic dynein to a spindle checkpoint protein, Bub3
    Kevin W H Lo
    Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville, Virginia 22908 0732, USA
    J Biol Chem 282:11205-12. 2007
    ....
  6. ncbi request reprint Methods to study the interactions of the dynein light chains and intermediate chains
    Kevin W H Lo
    Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA, USA
    Methods Mol Biol 392:85-95. 2007
    ..By using several complementary binding methods, we have investigated the protein-protein interaction in the cargo-binding domain of the cytoplasmic dynein...
  7. pmc A neuron-specific cytoplasmic dynein isoform preferentially transports TrkB signaling endosomes
    Junghoon Ha
    Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville, VA 22908, USA
    J Cell Biol 181:1027-39. 2008
    ..These data demonstrate that the IC isoforms define dynein populations that are selectively recruited to transport distinct cargoes...
  8. ncbi request reprint Intermediate chain subunit as a probe for cytoplasmic dynein function: biochemical analyses and live cell imaging in PC12 cells
    Kenneth R Myers
    Cell Biology Department, School of Medicine, University of Virginia, Charlottesville, Virginia, USA
    J Neurosci Res 85:2640-7. 2007
    ..Live cell imaging and comparative immunocytochemical analyses show that dynein is enriched in the actin rich region of growth cones...
  9. ncbi request reprint Identification of a novel region of the cytoplasmic Dynein intermediate chain important for dimerization in the absence of the light chains
    Kevin W H Lo
    Department of Cell Biology, University of Virginia, Charlottesville, Virginia 22908, USA
    J Biol Chem 281:9552-9. 2006
    ..Analytical gel filtration and covalent cross-linking of purified recombinant polypeptides further demonstrated that the intermediate chains can dimerize in vitro in the absence of the light chains...
  10. ncbi request reprint Interaction of the DYNLT (TCTEX1/RP3) light chains and the intermediate chains reveals novel intersubunit regulation during assembly of the dynein complex
    Kevin W H Lo
    Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville, Virginia 22908, USA
    J Biol Chem 282:36871-8. 2007
    ..Thus, whereas all combinations of DYNLT light chain dimers can be made, not all of the possible combinations of the isoforms are utilized during the assembly of the dynein complex...
  11. pmc Cytoplasmic dynein mediates adenovirus binding to microtubules
    Samir A Kelkar
    Weill Medical College of Cornell University, Department of Genetic Medicine, 515 E 71st St, S 1000, New York, NY 10021, USA
    J Virol 78:10122-32. 2004
    ..Partially uncoated capsids isolated from cells 40 min after infection also exhibited microtubule binding. In summary, the primary mode of Ad attachment to microtubules occurs though cytoplasmic dynein-mediated binding...
  12. ncbi request reprint The Roadblock light chains are ubiquitous components of cytoplasmic dynein that form homo- and heterodimers
    Karina Nikulina
    Department of Biochemistry, University of Connecticut Health Center, Farmington 06030 3305, USA
    Cell Motil Cytoskeleton 57:233-45. 2004
    ..Immunofluorescence analysis reveals that Robl is present in punctate organelles in rat neuroblastoma cells. In testis, Robl is found in Leydig cells, spermatocytes, and sperm flagella...
  13. ncbi request reprint Dynein cargo gets its groove back
    K Kevin Pfister
    Structure 13:172-3. 2005
    ..Recent work from the King lab (Wu et al., 2005) on the structure of the Tctex1 dynein light chain provides new insights into the mechanism of cytoplasmic dynein cargo binding and the functional significance of light chain isoform diversity...
  14. ncbi request reprint Viral strategies for intracellular trafficking: motors and microtubules
    Philip L Leopold
    Department of Genetic Medicine, Weill Medical College of Cornell University, New York, NY, USA
    Traffic 7:516-23. 2006
    ....

Research Grants18

  1. The Role of Cytoplasmic Dynein in Axonal Transport
    KEVIN K PFISTER; Fiscal Year: 2010
    ..Mutations in one motor, dynein, result in motor neurodegeneration. This project investigates how this motor attaches to cargo. ..
  2. The Role of Cytoplasmic Dynein in Axonal Transport
    KEVIN PFISTER; Fiscal Year: 2009
    ..Mutations in one motor, dynein, result in motor neurodegeneration. This project investigates how this motor attaches to cargo. ..
  3. The Role of Cytoplasmic Dynein in Axonal Transport
    KEVIN PFISTER; Fiscal Year: 2006
    ..These studies will contribute valuable information about the mechanism of axonal transport, a neuronal process important for axon growth and regeneration. ..
  4. ORGANELLE MOVEMENT IN FAST AXONAL TRANSPORT
    KEVIN PFISTER; Fiscal Year: 2001
    ..The phosphorylation state of dynein will be manipulated in vitro and the role of phosphorylation in regulating the properties of dynein will be determined. ..
  5. The Role of Cytoplasmic Dynein in Axonal Transport
    KEVIN PFISTER; Fiscal Year: 2009
    ..Mutations in one motor, dynein, result in motor neurodegeneration. This project investigates how this motor attaches to cargo. ..