HOWARD PETTY

Summary

Affiliation: University of Michigan
Country: USA

Publications

  1. pmc Toll-like receptor 4 (TLR4) of retinal pigment epithelial cells participates in transmembrane signaling in response to photoreceptor outer segments
    Andrei L Kindzelskii
    Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, Ann Arbor, MI 48105, USA
    J Gen Physiol 124:139-49. 2004
  2. ncbi request reprint Fluorescence microscopy: established and emerging methods, experimental strategies, and applications in immunology
    Howard R Petty
    Department of Ophthalmology and Visual Sciences, University of Michigan Medical School, Ann Arbor, Michigan 48105, USA
    Microsc Res Tech 70:687-709. 2007
  3. ncbi request reprint Spatiotemporal chemical dynamics in living cells: from information trafficking to cell physiology
    Howard R Petty
    Department of Ophthalmology, The University of Michigan Medical School, 1000 Wall Street, Ann Arbor, MI 48105, USA
    Biosystems 83:217-24. 2006
  4. ncbi request reprint Oxidant release is dramatically increased by elevated glucose concentrations in neutrophils from pregnant women
    Howard R Petty
    Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, Ann Arbor, MI, USA
    J Matern Fetal Neonatal Med 18:397-404. 2005
  5. ncbi request reprint Dynamic chemical instabilities in living cells may provide a novel route in drug development
    Howard R Petty
    Department of Ophthalmology, The University of Michigan Medical School, 1000 Wall Street, Ann Arbor, MI 48105, USA
    Chembiochem 5:1359-64. 2004
  6. pmc A facile method for immunofluorescence microscopy of highly autofluorescent human retinal sections using nanoparticles with large Stokes shifts
    Howard R Petty
    Department of Ophthalmology and Visual Sciences, University of Michigan Medical School, Ann Arbor, MI, United States
    J Neurosci Methods 191:222-6. 2010
  7. ncbi request reprint Trophoblast contact deactivates human neutrophils
    Howard R Petty
    Department of Ophthalmology and Visual Sciences, University of Michigan Medical School, Ann Arbor, MI 48105, USA
    J Immunol 176:3205-14. 2006
  8. ncbi request reprint TLR4 mediates human retinal pigment epithelial endotoxin binding and cytokine expression
    Susan G Elner
    Department of Ophthalmology, University of Michigan, W K Kellogg Eye Center, Ann Arbor 48105, USA
    Invest Ophthalmol Vis Sci 46:4627-33. 2005
  9. ncbi request reprint 6-phosphogluconate dehydrogenase and glucose-6-phosphate dehydrogenase form a supramolecular complex in human neutrophils that undergoes retrograde trafficking during pregnancy
    Andrei L Kindzelskii
    Department of Ophthalmology and Visual Sciences, University of Michigan Medical School, Ann Arbor, MI 48105, USA
    J Immunol 172:6373-81. 2004
  10. pmc TLR4 mediates human retinal pigment epithelial endotoxin binding and cytokine expression
    Susan G Elner
    Department of Ophthalmology, University of Michigan, Kellogg Eye Center, Ann Arbor, Michigan, USA
    Trans Am Ophthalmol Soc 103:126-35; discussion 135-7. 2005

Collaborators

Detail Information

Publications60

  1. pmc Toll-like receptor 4 (TLR4) of retinal pigment epithelial cells participates in transmembrane signaling in response to photoreceptor outer segments
    Andrei L Kindzelskii
    Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, Ann Arbor, MI 48105, USA
    J Gen Physiol 124:139-49. 2004
    ....
  2. ncbi request reprint Fluorescence microscopy: established and emerging methods, experimental strategies, and applications in immunology
    Howard R Petty
    Department of Ophthalmology and Visual Sciences, University of Michigan Medical School, Ann Arbor, Michigan 48105, USA
    Microsc Res Tech 70:687-709. 2007
    ..This review provides a broad integrative discussion of fluorescence microscopy with selected applications in immunology...
  3. ncbi request reprint Spatiotemporal chemical dynamics in living cells: from information trafficking to cell physiology
    Howard R Petty
    Department of Ophthalmology, The University of Michigan Medical School, 1000 Wall Street, Ann Arbor, MI 48105, USA
    Biosystems 83:217-24. 2006
    ..In this article I will review some of these basic findings, discuss these events in the context of information trafficking, and illustrate the potential implications of this work in medicine...
  4. ncbi request reprint Oxidant release is dramatically increased by elevated glucose concentrations in neutrophils from pregnant women
    Howard R Petty
    Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, Ann Arbor, MI, USA
    J Matern Fetal Neonatal Med 18:397-404. 2005
    ..To evaluate the mechanism of oxidative stress at glucose levels accompanying diabetic pregnancy. Specifically, we hypothesize that elevated glucose overwhelms hexose monophosphate shunt (HMS) down-regulation observed during pregnancy...
  5. ncbi request reprint Dynamic chemical instabilities in living cells may provide a novel route in drug development
    Howard R Petty
    Department of Ophthalmology, The University of Michigan Medical School, 1000 Wall Street, Ann Arbor, MI 48105, USA
    Chembiochem 5:1359-64. 2004
  6. pmc A facile method for immunofluorescence microscopy of highly autofluorescent human retinal sections using nanoparticles with large Stokes shifts
    Howard R Petty
    Department of Ophthalmology and Visual Sciences, University of Michigan Medical School, Ann Arbor, MI, United States
    J Neurosci Methods 191:222-6. 2010
    ..Of particular note is the ability to detect antigens within the brightly autofluorescent RPE cell layer...
  7. ncbi request reprint Trophoblast contact deactivates human neutrophils
    Howard R Petty
    Department of Ophthalmology and Visual Sciences, University of Michigan Medical School, Ann Arbor, MI 48105, USA
    J Immunol 176:3205-14. 2006
    ..This restriction in HMS activity deactivates neutrophils, thereby limiting oxidative DNA damage within trophoblasts...
  8. ncbi request reprint TLR4 mediates human retinal pigment epithelial endotoxin binding and cytokine expression
    Susan G Elner
    Department of Ophthalmology, University of Michigan, W K Kellogg Eye Center, Ann Arbor 48105, USA
    Invest Ophthalmol Vis Sci 46:4627-33. 2005
    ..This study was performed to demonstrate the classical role of TLR4 in HRPE endotoxin (lipopolysaccharide; LPS) binding leading to HRPE proinflammatory cytokine secretion...
  9. ncbi request reprint 6-phosphogluconate dehydrogenase and glucose-6-phosphate dehydrogenase form a supramolecular complex in human neutrophils that undergoes retrograde trafficking during pregnancy
    Andrei L Kindzelskii
    Department of Ophthalmology and Visual Sciences, University of Michigan Medical School, Ann Arbor, MI 48105, USA
    J Immunol 172:6373-81. 2004
    ....
  10. pmc TLR4 mediates human retinal pigment epithelial endotoxin binding and cytokine expression
    Susan G Elner
    Department of Ophthalmology, University of Michigan, Kellogg Eye Center, Ann Arbor, Michigan, USA
    Trans Am Ophthalmol Soc 103:126-35; discussion 135-7. 2005
    ..This study was performed to demonstrate the classic role of TLR4 in HRPE endotoxin (lipopolysaccharide [LPS]) binding leading to HRPE proinflammatory cytokine secretion...
  11. pmc Observation of calcium microdomains at the uropod of living morphologically polarized human neutrophils using flash lamp-based fluorescence microscopy
    Andrea J Clark
    Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, Ann Arbor, Michigan 48105, USA
    Cytometry A 73:673-8. 2008
    ..Calcium signaling at the uropod is likely associated with cytoskeletal remodeling during cell motility...
  12. ncbi request reprint Cutting edge: optical microspectrophotometry supports the existence of gel phase lipid rafts at the lamellipodium of neutrophils: apparent role in calcium signaling
    Andrei L Kindzelskii
    Department of Ophthalmology and Visual Sciences, University of Michigan Medical School, Ann Arbor, MI 48105, USA
    J Immunol 172:4681-5. 2004
    ..This is accompanied by disruption of Ca(2+) waves normally initiated at lamellipodia. Thus, m beta CD-sensitive lipid-ordered domains are present at and participate in signaling from the lamellipodia of living neutrophils...
  13. ncbi request reprint Stargardt-like macular dystrophy protein ELOVL4 exerts a dominant negative effect by recruiting wild-type protein into aggresomes
    Vidyullatha Vasireddy
    Department of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI, USA
    Mol Vis 11:665-76. 2005
    ..To understand the disease mechanism underlying STGD3 we studied the intracellular trafficking of the wild-type and a 5 bp deletion mutant of ELOVL4...
  14. ncbi request reprint Myeloperoxidase accumulates at the neutrophil surface and enhances cell metabolism and oxidant release during pregnancy
    Andrei L Kindzelskii
    Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, Ann Arbor, 48105, USA
    Eur J Immunol 36:1619-28. 2006
    ..MPO inhibition returned ROM and RNI formation to non-pregnant levels. Hence, MPO trafficking influences metabolic activity and oxidant production in pregnancy...
  15. ncbi request reprint Lipid raft compartmentalization of urokinase receptor signaling in human neutrophils
    Robert G Sitrin
    Pulmonary and Critical Medicine Division, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan 48109 0642, USA
    Am J Respir Cell Mol Biol 30:233-41. 2004
    ..We conclude that uPAR can engage distinct signaling pathways involving different partner proteins that are functionally and physically segregated from one another in both lipid raft and non-raft domains of the plasma membrane...
  16. ncbi request reprint Ceramide kinase promotes Ca2+ signaling near IgG-opsonized targets and enhances phagolysosomal fusion in COS-1 cells
    Vania Hinkovska-Galcheva
    Department of Pediatrics, Division of Hematology Oncology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    J Lipid Res 49:531-42. 2008
    ....
  17. ncbi request reprint Selective localization of recognition complexes for leukotriene B4 and formyl-Met-Leu-Phe within lipid raft microdomains of human polymorphonuclear neutrophils
    Robert G Sitrin
    Pulmonary and Critical Care Medicine Division, Department of Internal Medicine, University of Michigan, 1150 West Medical Drive, Ann Arbor, MI 48109, USA
    J Immunol 177:8177-84. 2006
    ..These findings highlight the complexity of chemotaxin signaling pathways and offer one mechanism by which neutrophils may spatially organize chemotaxin signaling within the plasma membrane...
  18. pmc An obligate role for membrane-associated neutral sphingomyelinase activity in orienting chemotactic migration of human neutrophils
    Robert G Sitrin
    Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan, Ann Arbor, 48109 5642, USA
    Am J Respir Cell Mol Biol 44:205-12. 2011
    ..We conclude that N-SMase at the cytofacial plasma membrane is an essential element for the proper orientation of PMNs in FMLP gradients, at least in part by polarizing the distribution of Rac 1/2 and RhoA GTPases...
  19. pmc Migrating human neutrophils exhibit dynamic spatiotemporal variation in membrane lipid organization
    Robert G Sitrin
    Pulmonary and Critical Care Medicine Division, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA
    Am J Respir Cell Mol Biol 43:498-506. 2010
    ..These findings solidify the existence of rapidly reorganizing LRMs in situ and support a role for LRMs in chemotaxin responsiveness...
  20. ncbi request reprint RPE CD14 immunohistochemical, genetic, and functional expression
    Victor M Elner
    Department of Ophthalmology, University of Michigan, W K Kellogg Eye Center, Ann Arbor, MI 48105, USA
    Exp Eye Res 76:321-31. 2003
    ..This study demonstrates that HRPE cells express functional CD14 in vitro and in situ along at the outer blood-retina barrier...
  21. pmc A cell permeant peptide containing the cytoplasmic tail sequence of Fc receptor type IIA reduces calcium signaling and phagolysosome formation in neutrophils
    Andrea J Clark
    Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, Ann Arbor, 48105, USA
    Cell Immunol 261:153-8. 2010
    ..These studies suggest that FcgammaRIIA's tail can act as a peptide decoy thereby blunting FcgammaRIIA-mediated processes, which, in turn, suggests a possible route in managing inflammatory tissue damage...
  22. ncbi request reprint Apparent role of dynein in glucose-6-phosphate dehydrogenase trafficking in neutrophils from pregnant women
    Ji Biao Huang
    Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, Ann Arbor, MI 48105, USA
    Metabolism 55:279-81. 2006
    ..On this basis, we conclude that microtubule motor proteins participate in hexose monophosphate shunt enzyme transport within leukocytes...
  23. pmc Calicum microdomains form within neutrophils at the neutrophil-tumor cell synapse: role in antibody-dependent target cell apoptosis
    Andrea J Clark
    Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, 1000 Wall Street, Ann Arbor, MI 48105, USA
    Cancer Immunol Immunother 59:149-59. 2010
    ..As Ca(2+) signals within neutrophils were necessary for specific tumor cell apoptosis, a central role of microdomains in leukocyte-mediated tumor cell destruction is indicated...
  24. ncbi request reprint Transaldolase is part of a supramolecular complex containing glucose-6-phosphate dehydrogenase in human neutrophils that undergoes retrograde trafficking during pregnancy
    Ji Biao Huang
    Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, Ann Arbor, MI 48105, USA
    Metabolism 54:1027-33. 2005
    ....
  25. ncbi request reprint Ion channel clustering enhances weak electric field detection by neutrophils: apparent roles of SKF96365-sensitive cation channels and myeloperoxidase trafficking in cellular responses
    Andrei L Kindzelskii
    Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, 1000 Wall Street, Ann Arbor, MI 48105, USA
    Eur Biophys J 35:1-26. 2005
    ..In addition to providing new mechanistic insights concerning electric field interactions with cells, our work suggests novel methods to remotely manipulate physiological pathways...
  26. pmc Human RPE cell apoptosis induced by activated monocytes is mediated by caspase-3 activation
    Susan G Elner
    Department of Ophthalmology, University of Michigan, Ann Arbor, USA
    Trans Am Ophthalmol Soc 101:77-89; discussion 89-91. 2003
    ..To determine the effects of activated monocytes on the induction of human retinal pigment epithelial (HRPE) cell reactive oxygen metabolite (ROM) production and apoptosis...
  27. ncbi request reprint Identification of channels promoting calcium spikes and waves in HT1080 tumor cells: their apparent roles in cell motility and invasion
    Ji Biao Huang
    Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, Ann Arbor, Michigan 48105, USA
    Cancer Res 64:2482-9. 2004
    ..In addition to providing novel information regarding cancer cell motility, we suggest that it may be possible to design drugs that inhibit a key Ca(2+) wave, thereby enhancing the efficacy of emerging therapeutic protocols...
  28. ncbi request reprint Pericellular proteolysis by leukocytes and tumor cells on substrates: focal activation and the role of urokinase-type plasminogen activator
    Andrei L Kindzelskii
    Department of Ophthalmology and Visual Sciences and Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48105, USA
    Histochem Cell Biol 121:299-310. 2004
    ..demonstrated its usefulness in real-time studies of cell migration, and (4). showed that the uPA/uPAR system is an important contributor to focal pericellular proteolysis...
  29. ncbi request reprint Activated monocytes induce human retinal pigment epithelial cell apoptosis through caspase-3 activation
    Ayako Yoshida
    Department of Ophthalmology, University of Michigan, Ann Arbor, Michigan 48105, USA
    Lab Invest 83:1117-29. 2003
    ..These mechanisms may compromise HRPE cell function and survival in a variety of retinal diseases...
  30. pmc Leukocyte pyruvate kinase expression is reduced in normal human pregnancy but not in pre-eclampsia
    Yi Xu
    Department of Obstetrics and Gynecology, Wayne State University, Detroit, MI, USA
    Am J Reprod Immunol 64:137-51. 2010
    ..To examine the immunoregulatory role of glycolysis in pregnancy, we evaluated the properties of pyruvate kinase in leukocytes from non-pregnant women and those with normal pregnancy and pre-eclampsia...
  31. ncbi request reprint Signal processing times in neutrophil activation: dependence on ligand concentration and the relative phase of metabolic oscillations
    Eric Albrecht
    Department of Ophthalmology and Visual Science, The University of Michigan School of Medicine, 1000 Wall Street, Ann Arbor, MI 48105, USA
    Biophys Chem 106:211-9. 2003
    ..Our experiments show that the kinetics of transmembrane signal processing, in contrast to a simple transmembrane chemical reaction, can depend upon both ligand dose and its temporal relationship with intracellular metabolic oscillations...
  32. pmc Pro-inflammatory cytokines increase reactive oxygen species through mitochondria and NADPH oxidase in cultured RPE cells
    Dongli Yang
    Department of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI 48105 0714, USA
    Exp Eye Res 85:462-72. 2007
    ..05). Collectively, these results demonstrate that TNF-alpha, IL-1 beta and IFN-gamma increase mitochondrial- and NADPH oxidase-generated ROS in human RPE cells...
  33. pmc An enzymatic fluorimetric assay for glucose-6-phosphate: application in an in vitro Warburg-like effect
    Aiping Zhu
    Department of Ophthalmology and Visual Sciences, University of Michigan Medical School, 1000 Wall Street, Ann Arbor, MI 48105, USA
    Anal Biochem 388:97-101. 2009
    ..After 2h of incubation at 37 degrees C, G6P levels rose by 20%, thereby illustrating an in vitro Warburg-like effect on cell metabolism...
  34. ncbi request reprint Differential intracellular distributions of inositol trisphosphate and ryanodine receptors within and among hematopoietic cells
    Andrea J Clark
    Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, 1000 Wall Street, Ann Arbor, MI 48105, USA
    J Histochem Cytochem 53:913-6. 2005
    ..We suggest that the observed differences influence intracellular Ca(2+) signaling...
  35. ncbi request reprint Human RPE cell lysis of extracellular matrix: functional urokinase plasminogen activator receptor (uPAR), collagenase and elastase
    Susan G Elner
    Department of Ophthalmology, University of Michigan, Kellogg Eye Center, Ann Arbor, MI 48105, USA
    Exp Eye Res 76:585-95. 2003
    ..Our results suggest that multiple proteolytic systems are present in association with HRPE and that the uPAR/uPA system may be particularly important...
  36. ncbi request reprint Fluorescence spectroscopic detection of mitochondrial flavoprotein redox oscillations and transient reduction of the NADPH oxidase-associated flavoprotein in leukocytes
    Andrei Kindzelskii
    Department of Ophthalmology and Visual Science, The University of Michigan School of Medicine, 1000 Wall Street, Ann Arbor, MI 48105, USA
    Eur Biophys J 33:291-9. 2004
    ..This finding is consistent with prior studies indicating that propagating substrate (NADPH) waves periodically promote electron transport across the NADPH oxidase...
  37. pmc Association of superoxide anions with retinal pigment epithelial cell apoptosis induced by mononuclear phagocytes
    Dongli Yang
    Department of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, Michigan 48105 0714, USA
    Invest Ophthalmol Vis Sci 50:4998-5005. 2009
    ..The purpose of this study was to determine the role of superoxide anions (O(2)(-)) in mononuclear phagocyte-induced RPE apoptosis...
  38. pmc Rapid, noninvasive detection of diabetes-induced retinal metabolic stress
    Matthew G Field
    Department of Ophthalmology and Visual Sciences, University of Michigan, 1000 Wall St, Ann Arbor, MI 48105, USA
    Arch Ophthalmol 126:934-8. 2008
    ..To test whether subjects with diabetes mellitus (DM) have enhanced retinal flavoprotein autofluorescence compared with age-matched control subjects using a rapid, noninvasive clinical imaging method...
  39. pmc 2,5-Deoxyfructosazine, a D-glucosamine derivative, inhibits T-cell interleukin-2 production better than D-glucosamine
    Aiping Zhu
    Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, Ann Arbor, MI 48105, United States
    Carbohydr Res 342:2745-9. 2007
    ..Hence, fructosazines constitute a novel class of immunomodulators...
  40. ncbi request reprint Ultrasensitive detection of DNA damage by the combination of the comet and TUNEL assays
    Andrei L Kindzelskii
    Department of Biological Sciences, Wayne State University, Detroit, MI, USA
    Methods Mol Biol 203:195-201. 2002
  41. ncbi request reprint Molecular proximity of complement receptor type 3 (CR3) and the glycosylphosphatidylinositol-linked protein GPI-80 on neutrophils: effects of cell adherence, exogenous saccharides, and lipid raft disrupting agents
    Ji Biao Huang
    Department of Ophthalmology and Visual Science, University of Michigan Medical School, 1000 Wall Street, Ann Arbor, MI 48105, USA
    Mol Immunol 40:1249-56. 2004
    ..Thus, lipid rafts may be important for the physical and functional cooperation of CR3 and GPI-80...
  42. ncbi request reprint Molecular proximity of seprase and the urokinase-type plasminogen activator receptor on malignant melanoma cell membranes: dependence on beta1 integrins and the cytoskeleton
    Vira V Artym
    Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA
    Carcinogenesis 23:1593-601. 2002
    ..Based on these findings, we speculate that formation of heterogeneous lytic domains in the invading membranes of LOX cells increases the efficiency of directed pericellular proteolysis...
  43. ncbi request reprint Cutting edge: fever-associated temperatures enhance neutrophil responses to lipopolysaccharide: a potential mechanism involving cell metabolism
    Allen J Rosenspire
    Department of Biological Sciences, Wayne State University, Detroit, MI 48202
    J Immunol 169:5396-400. 2002
    ....
  44. ncbi request reprint Function of the lectin domain of Mac-1/complement receptor type 3 (CD11b/CD18) in regulating neutrophil adhesion
    Yu Xia
    Chemoattractant Group of the James Graham Brown Cancer Center, Department of Pathology, University of Louisville, KY 40202, USA
    J Immunol 169:6417-26. 2002
    ..These data indicate that the same lectin domain within CD11b regulates both the cytotoxic and adhesion functions of Mac-1/CR3...
  45. pmc Apparent role of traveling metabolic waves in oxidant release by living neutrophils
    Andrei L Kindzelskii
    Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA
    Proc Natl Acad Sci U S A 99:9207-12. 2002
    ..Propagating NAD(P)H waves allow neutrophils to specifically deliver substrate to the lamellipodium at high concentrations, thus facilitating the local and periodic release of ROMs in the direction of cell movement and/or a target...
  46. ncbi request reprint Hexokinase translocation during neutrophil activation, chemotaxis, and phagocytosis: disruption by cytochalasin D, dexamethasone, and indomethacin
    Ji Biao Huang
    Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA
    Cell Immunol 218:95-106. 2002
    ..Parallel regulation of reactive oxygen metabolite (ROM) production was shown. We speculate that hexokinase translocation participates in neutrophil activation...
  47. ncbi request reprint Ceramide 1-phosphate, a mediator of phagocytosis
    Vania Hinkovska-Galcheva
    Department of Pediatrics, Division of Hematology Oncology, Virginia Commonwealth University School of Medicine, Richmond, Virginia 23298, USA
    J Biol Chem 280:26612-21. 2005
    ..The change in the structural order parameter of the lipid rafts probably contributes to phagocytosis by promoting phagosome formation...
  48. pmc Pregnancy alters glucose-6-phosphate dehydrogenase trafficking, cell metabolism, and oxidant release of maternal neutrophils
    Andrei L Kindzelskii
    Department of Biological Sciences, Wayne State University, Detroit, Michigan 48202, USA
    J Clin Invest 110:1801-11. 2002
    ..G-6-PDase trafficking appears to be one mechanism regulating ROM production by maternal neutrophils...
  49. pmc Real-time control of neutrophil metabolism by very weak ultra-low frequency pulsed magnetic fields
    Allen J Rosenspire
    Department of Biological Sciences, Wayne State University, Detroit, Michigan 48202, USA
    Biophys J 88:3334-47. 2005
    ..We estimate that the threshold which induced electric fields must attain to influence cell metabolism is of the order of 10(-4) V/m...
  50. pmc Quantification of O-GlcNAc protein modification in neutrophils by flow cytometry
    Sally A Madsen-Bouterse
    Perinatology Research Branch, NICHD NIH DHHS, Bethesda, Maryland 20892, USA
    Cytometry A 73:667-72. 2008
    ..Neutrophil protein modifications by O-GlcNAc are rapidly detected using flow cytometry and provide information similar to that observed using fluorescence microscopy...
  51. ncbi request reprint Intracellular calcium waves accompany neutrophil polarization, formylmethionylleucylphenylalanine stimulation, and phagocytosis: a high speed microscopy study
    Andrei L Kindzelskii
    Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA
    J Immunol 170:64-72. 2003
    ....
  52. pmc Evidence for chronic, peripheral activation of neutrophils in polyarticular juvenile rheumatoid arthritis
    James N Jarvis
    Department of Pediatrics, University of Oklahoma College of Medicine, 940 Stanton L, Young Blvd, Oklahoma City, OK 73104, USA
    Arthritis Res Ther 8:R154. 2006
    ..Our data are consistent with the hypothesis that neutrophils play an essential role in the pathogenesis of polyarticular JRA...
  53. ncbi request reprint Detection of cytokine signal transduction "cross-talk" in leukocyte activation
    Howard R Petty
    Department of Biological Sciences, Wayne State University, Detroit, Michigan, USA
    Methods Mol Biol 249:219-28. 2004
  54. ncbi request reprint Interactions of integrins with their partner proteins in leukocyte membranes
    Howard R Petty
    Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA
    Immunol Res 25:75-95. 2002
    ..In this article we will highlight certain features of this growing field of research, especially with regard to their relevance in immunology and inflammation...
  55. pmc Elevated glucose concentrations promote receptor-independent activation of adherent human neutrophils: an experimental and computational approach
    Ursula Kummer
    Bioinformatics and Computational Biochemistry, EML Research, Heidelberg, Germany
    Biophys J 92:2597-607. 2007
    ....
  56. pmc Signal sequence within Fc gamma RIIA controls calcium wave propagation patterns: apparent role in phagolysosome fusion
    Randall G Worth
    Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 100:4533-8. 2003
    ....
  57. ncbi request reprint Human, but not bovine, photoreceptor outer segments prime human retinal pigment epithelial cells for metabolic activation and massive oxidant release in response to lipopolysaccharide and interferon-gamma
    Andrei L Kindzelskii
    Exp Eye Res 79:431-5. 2004
    ..However, no ROM priming of human RPE cells is observed for bovine POS. ROM production appears to be linked with underlying metabolic oscillations involving the hexose monophosphate shunt...
  58. pmc A model of the oscillatory metabolism of activated neutrophils
    Lars F Olsen
    European Media Laboratory, Schloss Wolfsbrunnenweg 33, D 69118 Heidelberg, Germany
    Biophys J 84:69-81. 2003
    ..Successful computer modeling of complex chemical dynamics within cells and their chemical perturbation will enhance our ability to identify new antiinflammatory compounds...
  59. doi request reprint Overview of the physical state of proteins within cells
    Howard R Petty
    Wayne State University, Detroit, Michigan, USA
    Curr Protoc Protein Sci . 2003
    ....
  60. ncbi request reprint IFN-gamma primes RAW264 macrophages and human monocytes for enhanced oxidant production in response to CpG DNA via metabolic signaling: roles of TLR9 and myeloperoxidase trafficking
    Yoshiyuki Adachi
    Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan
    J Immunol 176:5033-40. 2006
    ..We suggest that amplitude and frequency modulation of cellular metabolic oscillations contribute to intracellular signaling synergy...

Research Grants26

  1. Mechanisms Regulating Neutrophil Activation in Pregnancy
    HOWARD PETTY; Fiscal Year: 2002
    ..abstract_text> ..
  2. ELF ELECTROMAGNETIC FIELDS AND CANCER
    HOWARD PETTY; Fiscal Year: 2002
    ..Moreover, the ability of electric fields to remotely influence cell shape and metabolism may be of broad importance in human health. ..
  3. Mechanisms Regulating Neutrophil Activation in Pregnancy
    HOWARD PETTY; Fiscal Year: 2004
    ..abstract_text> ..
  4. WATCHING TARGET CELL OXIDATION AND CYTOLYSIS
    HOWARD PETTY; Fiscal Year: 1993
    ..The insights gained from this research program may be useful in developing rational drug therapies to control inflammatory diseases...
  5. Signaling Dynamics of Leukocyte-Tumor Cell Interactions
    HOWARD PETTY; Fiscal Year: 2005
    ..These new insights are likely to lead to advances in immunotherapy of cancer. ..
  6. WATCHING TARGET CELL OXIDATION AND CYTOLYSIS
    HOWARD PETTY; Fiscal Year: 1990
    ..The oxidation of tumor targets during antibody-independent cytotoxicity will also be studied...
  7. WATCHING TARGET CELL OXIDATION AND CYTOLYSIS
    HOWARD PETTY; Fiscal Year: 1992
    ..The insights gained from this research program may be useful in developing rational drug therapies to control inflammatory diseases...
  8. Mechanisms Regulating Neutrophil Activation in Pregnancy
    HOWARD PETTY; Fiscal Year: 2003
    ..abstract_text> ..
  9. WATCHING TARGET CELL OXIDATION AND CYTOLYSIS
    HOWARD PETTY; Fiscal Year: 1999
    ..Our studies will determine the nature and mechanisms of inter-receptor interactions, which may lead to the development of new anti-inflammatory agents. ..
  10. Signaling Dynamics of Leukocyte-Tumor Cell Interactions
    HOWARD PETTY; Fiscal Year: 2006
    ..These new insights are likely to lead to advances in immunotherapy of cancer. ..
  11. Mechanisms Regulating Neutrophil Activation in Pregnancy
    HOWARD PETTY; Fiscal Year: 2005
    ..abstract_text> ..
  12. Signaling Dynamics of Leukocyte-Tumor Cell Interactions
    HOWARD PETTY; Fiscal Year: 2007
    ..These new insights are likely to lead to advances in immunotherapy of cancer. ..
  13. ELF ELECTROMAGNETIC FIELDS AND CANCER
    HOWARD PETTY; Fiscal Year: 2003
    ..Moreover, the ability of electric fields to remotely influence cell shape and metabolism may be of broad importance in human health. ..
  14. Mechanisms Regulating Neutrophil Activation in Pregnancy
    HOWARD PETTY; Fiscal Year: 2002
    ..abstract_text> ..
  15. ELF ELECTROMAGNETIC FIELDS AND CANCER
    HOWARD PETTY; Fiscal Year: 2004
    ..Moreover, the ability of electric fields to remotely influence cell shape and metabolism may be of broad importance in human health. ..
  16. WATCHING TARGET CELL OXIDATION AND CYTOLYSIS
    HOWARD PETTY; Fiscal Year: 2001
    ..Our studies will determine the nature and mechanisms of inter-receptor interactions, which may lead to the development of new anti-inflammatory agents. ..
  17. ELF ELECTROMAGNETIC FIELDS AND CANCER
    HOWARD PETTY; Fiscal Year: 2000
    ..A mechanistic series of events linking ELF fields with adherence/locomotion/invasive properties of cancer cells, metabolism and DNA damage will be identified; this may provide a physicochemical basis for ELF health effects. ..
  18. WATCHING TARGET CELL OXIDATION AND CYTOLYSIS
    HOWARD PETTY; Fiscal Year: 2000
    ..Our studies will determine the nature and mechanisms of inter-receptor interactions, which may lead to the development of new anti-inflammatory agents. ..
  19. WATCHING TARGET CELL OXIDATION AND CYTOLYSIS
    HOWARD PETTY; Fiscal Year: 1991
    ..The oxidation of tumor targets during antibody-independent cytotoxicity will also be studied...
  20. ELF ELECTROMAGNETIC FIELDS AND CANCER
    HOWARD PETTY; Fiscal Year: 1999
    ..A mechanistic series of events linking ELF fields with adherence/locomotion/invasive properties of cancer cells, metabolism and DNA damage will be identified; this may provide a physicochemical basis for ELF health effects. ..