KENNETH PETERSON

Summary

Affiliation: University of Kansas Medical Center
Country: USA

Publications

  1. pmc Mi2β is required for γ-globin gene silencing: temporal assembly of a GATA-1-FOG-1-Mi2 repressor complex in β-YAC transgenic mice
    Flavia C Costa
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, Kansas, USA
    PLoS Genet 8:e1003155. 2012
  2. pmc Induction of Fetal Hemoglobin In Vivo Mediated by a Synthetic γ-Globin Zinc Finger Activator
    Flavia C Costa
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
    Anemia 2012:507894. 2012
  3. pmc LCR 5' hypersensitive site specificity for globin gene activation within the active chromatin hub
    Kenneth R Peterson
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Nucleic Acids Res 40:11256-69. 2012
  4. pmc Rapid isolation of yeast genomic DNA: Bust n' Grab
    Susanna Harju
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    BMC Biotechnol 4:8. 2004
  5. ncbi request reprint Preparation of intact yeast artificial chromosome DNA for transgenesis of mice
    Kenneth R Peterson
    Department of Biochemistry and Molecular Biology, Mail Stop 3030, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, Kansas 66160, USA
    Nat Protoc 2:3009-15. 2007
  6. ncbi request reprint LCR-dependent gene expression in beta-globin YAC transgenics: detailed structural studies validate functional analysis even in the presence of fragmented YACs
    K R Peterson
    Division of Medical Genetics, Department of Medicine and Department of Genetics, University of Washington, Seattle, WA 98195, USA
    Hum Mol Genet 7:2079-88. 1998
  7. ncbi request reprint Transgenic Cre expression mice for generation of erythroid-specific gene alterations
    Kenneth R Peterson
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, Kansas 66160 7421, USA
    Genesis 39:1-9. 2004
  8. ncbi request reprint Transgenic mice carrying yeast artificial chromosomes
    Kenneth R Peterson
    Department of Biochemistry and Molecular Biology, 3901 Rainbow Blvd, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Expert Rev Mol Med 5:1-25. 2003
  9. ncbi request reprint Hemoglobin switching: new insights
    Kenneth R Peterson
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    Curr Opin Hematol 10:123-9. 2003
  10. ncbi request reprint Activation of the beta-like globin genes in transgenic mice is dependent on the presence of the beta-locus control region
    Patrick A Navas
    Division of Medical Genetics, University of Washington, Seattle, WA 98195, USA
    Hum Mol Genet 11:893-903. 2002

Research Grants

  1. Transactivation of Fetal Hemoglobin
    Kenneth R Peterson; Fiscal Year: 2010
  2. Transactivation of Fetal Hemoglobin
    Kenneth R Peterson; Fiscal Year: 2010
  3. Transactivation of Fetal Hemoglobin
    KENNETH PETERSON; Fiscal Year: 2009
  4. Regulation of Globin Gene Switching in Human ES Cells
    KENNETH PETERSON; Fiscal Year: 2003
  5. Locus-linked Regulatory Motifs of Globin Gene Switching
    KENNETH PETERSON; Fiscal Year: 2003
  6. Locus-linked Regulator Motifs of Globin Gene Switching
    KENNETH PETERSON; Fiscal Year: 2001
  7. LCR AND HUMAN BETA GLOBIN GENE REGULATION
    KENNETH PETERSON; Fiscal Year: 1999
  8. ROLE OF THE LCR IN HUMAN BETA-GLOBIN GENE REGULATION
    KENNETH PETERSON; Fiscal Year: 2001
  9. Transactivation of Fetal Hemoglobin
    KENNETH PETERSON; Fiscal Year: 2009
  10. Molecular control of fetal G-globin gene expression
    KENNETH PETERSON; Fiscal Year: 2004

Collaborators

Detail Information

Publications23

  1. pmc Mi2β is required for γ-globin gene silencing: temporal assembly of a GATA-1-FOG-1-Mi2 repressor complex in β-YAC transgenic mice
    Flavia C Costa
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, Kansas, USA
    PLoS Genet 8:e1003155. 2012
    ....
  2. pmc Induction of Fetal Hemoglobin In Vivo Mediated by a Synthetic γ-Globin Zinc Finger Activator
    Flavia C Costa
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
    Anemia 2012:507894. 2012
    ..Our results demonstrate that ATFs function in an animal model to increase gene expression. Thus, this class of reagent may be an effective gene therapy for treatment of some inherited diseases...
  3. pmc LCR 5' hypersensitive site specificity for globin gene activation within the active chromatin hub
    Kenneth R Peterson
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Nucleic Acids Res 40:11256-69. 2012
    ..These data support a site specificity model of LCR HS-globin gene interaction...
  4. pmc Rapid isolation of yeast genomic DNA: Bust n' Grab
    Susanna Harju
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    BMC Biotechnol 4:8. 2004
    ..We sought to develop an easier and faster protocol than the existing methods for obtaining yeast genomic DNA from liquid cultures or colonies on plates...
  5. ncbi request reprint Preparation of intact yeast artificial chromosome DNA for transgenesis of mice
    Kenneth R Peterson
    Department of Biochemistry and Molecular Biology, Mail Stop 3030, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, Kansas 66160, USA
    Nat Protoc 2:3009-15. 2007
    ..Special precautions are also taken during the microinjection protocol. Transgenesis efficiency is approximately 15%; most animals carry 1-5 copies of the desired locus. This method takes 6 d for completion...
  6. ncbi request reprint LCR-dependent gene expression in beta-globin YAC transgenics: detailed structural studies validate functional analysis even in the presence of fragmented YACs
    K R Peterson
    Division of Medical Genetics, Department of Medicine and Department of Genetics, University of Washington, Seattle, WA 98195, USA
    Hum Mol Genet 7:2079-88. 1998
    ..However, detailed structural evidence for integrity and continuity of locus sequences is required for correct interpretation of functional data...
  7. ncbi request reprint Transgenic Cre expression mice for generation of erythroid-specific gene alterations
    Kenneth R Peterson
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, Kansas 66160 7421, USA
    Genesis 39:1-9. 2004
    ..We demonstrate that beta(S) expression was not detected in the blood of bigenics, but the gene was present in nonerythroid cells. Thus, excision of the loxP-flanked beta(S) gene was restricted to erythroid cell lineages...
  8. ncbi request reprint Transgenic mice carrying yeast artificial chromosomes
    Kenneth R Peterson
    Department of Biochemistry and Molecular Biology, 3901 Rainbow Blvd, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Expert Rev Mol Med 5:1-25. 2003
    ..Application of this technology to develop human artificial chromosomes (HACs) might provide the next generation of gene therapy vectors that will overcome most of the problems and barriers associated with current vector systems...
  9. ncbi request reprint Hemoglobin switching: new insights
    Kenneth R Peterson
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    Curr Opin Hematol 10:123-9. 2003
    ..The studies reviewed here contribute new insights on the interplay between -acting elements, transcription factors, and chromatin modifiers that underlie globin gene switching during development...
  10. ncbi request reprint Activation of the beta-like globin genes in transgenic mice is dependent on the presence of the beta-locus control region
    Patrick A Navas
    Division of Medical Genetics, University of Washington, Seattle, WA 98195, USA
    Hum Mol Genet 11:893-903. 2002
    ....
  11. pmc Synergistic and additive properties of the beta-globin locus control region (LCR) revealed by 5'HS3 deletion mutations: implication for LCR chromatin architecture
    Xiangdong Fang
    Medical Genetics, University of Washington, Seattle, 98195, USA
    Mol Cell Biol 25:7033-41. 2005
    ..Based on these results we elaborate on a model of LCR chromatin architecture which accommodates the distinct phenotypes of the 5'HS3 and HS3 core deletions...
  12. pmc Silencing of Agamma-globin gene expression during adult definitive erythropoiesis mediated by GATA-1-FOG-1-Mi2 complex binding at the -566 GATA site
    Susanna Harju-Baker
    Department of Biochemistry and Molecular Biology, MS 3030, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA
    Mol Cell Biol 28:3101-13. 2008
    ....
  13. ncbi request reprint Chromatin structure and control of beta-like globin gene switching
    Susanna Harju
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City 66160, USA
    Exp Biol Med (Maywood) 227:683-700. 2002
    ..In this review we attempt to unify the current knowledge regarding the interplay of cis-acting elements, transcription factors, and chromatin modifiers into a comprehensive overview of globin gene switching...
  14. pmc Investigations of a human embryonic globin gene silencing element using YAC transgenic mice
    Patrick A Navas
    Division of Medical Genetics, University of Washington, Seattle, 98195, USA
    Exp Biol Med (Maywood) 231:328-34. 2006
    ....
  15. pmc Locus control regions
    Qiliang Li
    Division of Medical Genetics, Department of Genome Sciences, University of Washington, 1705 NE Pacific Street, Seattle, WA 98195, USA
    Blood 100:3077-86. 2002
    ....
  16. pmc Deletion of the human beta-globin LCR 5'HS4 or 5'HS1 differentially affects beta-like globin gene expression in beta-YAC transgenic mice
    Halyna Fedosyuk
    Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Blood Cells Mol Dis 39:44-55. 2007
    ....
  17. ncbi request reprint Establishment of cell lines that exhibit correct ontogenic stage-specific gene expression profiles from tissues of yeast artificial chromosome transgenic mice using chemically induced growth signals
    C Anthony Blau
    Division of Hematology, University of Washington, Seattle, USA
    Methods Mol Biol 349:163-73. 2006
    ..This approach extends the usefulness of YAC transgenic mice for the generation of cell lines amenable to more detailed studies regarding gene regulation...
  18. pmc Genome architecture of the human beta-globin locus affects developmental regulation of gene expression
    Susanna Harju
    Department of Biochemistry and Molecular Biology, MS 3030, University of Kansas Medical Center, Kansas City, 66160 7421, USA
    Mol Cell Biol 25:8765-78. 2005
    ..These results indicate that distance from the LCR, an inherent property of spatial gene order, is a major determinant of temporal gene expression during development...
  19. pmc A FTZ-F1-containing yeast artificial chromosome recapitulates expression of steroidogenic factor 1 in vivo
    Tatiana Karpova
    Department of Molecular and Integrative Physiology, The University of Kansas Medical Center, 3901 Rainbow Boulevard Kansas City, Kansas 66160, USA
    Mol Endocrinol 19:2549-63. 2005
    ..Thus, the study defines a region of Ftz-F1 that contains the requisite set of regulatory elements to direct SF-1 cell-specific expression and all temporal and quantitative changes need for its biological activity...
  20. pmc Mutation of a transcriptional motif of a distant regulatory element reduces the expression of embryonic and fetal globin genes
    Patrick A Navas
    Division of Medical Genetics, University of Washington, Seattle, 98195, USA
    Hum Mol Genet 12:2941-8. 2003
    ..Our results provide evidence that mutations of single transcriptional motifs of distant regulatory elements can have profound effects on gene expression...
  21. ncbi request reprint {gamma}-Globin gene expression in chemical inducer of dimerization (CID)-dependent multipotential cells established from human {beta}-globin locus yeast artificial chromosome ({beta}-YAC) transgenic mice
    C Anthony Blau
    Division of Hematology, Department of Medicine, University of Washington Medical Center, Seattle, Washington 98195, USA
    J Biol Chem 280:36642-7. 2005
    ..These cells may be useful for identifying transcription factors that reactivate gamma-globin synthesis or screening gamma-globin inducers for the treatment of sickle cell disease or beta-thalassemia...
  22. ncbi request reprint Identification of novel candidate genes for globin regulation in erythroid cells containing large deletions of the human beta-globin gene cluster
    Tiago Gomes De Andrade
    Institute of Medical and Biological Sciences, Federal University of Alagoas, Maceio, AL, Brazil
    Blood Cells Mol Dis 37:82-90. 2006
    ..Interestingly, alpha-globin mRNA levels were decreased, similar to beta-globin in all reticulocyte samples analyzed...
  23. pmc Correction of a splice-site mutation in the beta-globin gene stimulated by triplex-forming peptide nucleic acids
    Joanna Y Chin
    Departments of Therapeutic Radiology and Genetics, Yale University School of Medicine, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 105:13514-9. 2008
    ..This work suggests that PNAs can be effective tools to induce heritable, site-specific modification of disease-related genes in human cells...

Research Grants20

  1. Transactivation of Fetal Hemoglobin
    Kenneth R Peterson; Fiscal Year: 2010
    ....
  2. Transactivation of Fetal Hemoglobin
    Kenneth R Peterson; Fiscal Year: 2010
    ....
  3. Transactivation of Fetal Hemoglobin
    KENNETH PETERSON; Fiscal Year: 2009
    ....
  4. Regulation of Globin Gene Switching in Human ES Cells
    KENNETH PETERSON; Fiscal Year: 2003
    ..Specific Aim 4 will analyze the expression of the murine B-like globin genes in human cells to better understand the evolutionary relationships and conservation of gene regulation and expression patterns between these two species. ..
  5. Locus-linked Regulatory Motifs of Globin Gene Switching
    KENNETH PETERSON; Fiscal Year: 2003
    ....
  6. Locus-linked Regulator Motifs of Globin Gene Switching
    KENNETH PETERSON; Fiscal Year: 2001
    ....
  7. LCR AND HUMAN BETA GLOBIN GENE REGULATION
    KENNETH PETERSON; Fiscal Year: 1999
    ..Specific Aim 3 will test the role of the LCR sequences in LCR complex formation and globin gene regulation using transgenic mice containing mutant beta-globin loci by applying FRET. ..
  8. ROLE OF THE LCR IN HUMAN BETA-GLOBIN GENE REGULATION
    KENNETH PETERSON; Fiscal Year: 2001
    ..Specific Aim 3 will test the role of the LCR sequences in LCR complex formation and globin gene regulation using transgenic mice containing mutant beta-globin loci by applying FRET. ..
  9. Transactivation of Fetal Hemoglobin
    KENNETH PETERSON; Fiscal Year: 2009
    ....
  10. Molecular control of fetal G-globin gene expression
    KENNETH PETERSON; Fiscal Year: 2004
    ....
  11. ROLE OF THE LCR IN HUMAN BETA-GLOBIN GENE REGULATION
    KENNETH PETERSON; Fiscal Year: 2000
    ..Specific Aim 3 will test the role of the LCR sequences in LCR complex formation and globin gene regulation using transgenic mice containing mutant beta-globin loci by applying FRET. ..
  12. Molecular control of fetal G-globin gene expression
    KENNETH PETERSON; Fiscal Year: 2001
    ....
  13. Molecular control of fetal G-globin gene expression
    KENNETH PETERSON; Fiscal Year: 2003
    ....
  14. Transactivation of Fetal Hemoglobin
    KENNETH PETERSON; Fiscal Year: 2007
    ..This proposal will generate unique tools and new strategies to understand mechanisms of ?-globin gene regulation for treatment of SCD. ..
  15. Locus-linked Regulatory Motifs of Globin Gene Switching
    KENNETH PETERSON; Fiscal Year: 2004
    ....
  16. Locus-linked Regulatory Motifs of Globin Gene Switching
    KENNETH PETERSON; Fiscal Year: 2002
    ....
  17. Molecular control of fetal G-globin gene expression
    KENNETH PETERSON; Fiscal Year: 2002
    ....