ROBERT DONALD PERRY

Summary

Affiliation: University of Kentucky
Country: USA

Publications

  1. pmc Reduced synthesis of the Ybt siderophore or production of aberrant Ybt-like molecules activates transcription of yersiniabactin genes in Yersinia pestis
    M Clarke Miller
    Department of Chemistry, University of Kentucky, Lexington, KY 40506 0055, USA
    Microbiology 156:2226-38. 2010
  2. pmc Manganese transporters Yfe and MntH are Fur-regulated and important for the virulence of Yersinia pestis
    Robert D Perry
    Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY 40536 0298, USA
    Microbiology 158:804-15. 2012
  3. pmc Yersiniabactin iron uptake: mechanisms and role in Yersinia pestis pathogenesis
    Robert D Perry
    Department of Microbiology, Immunology, and Molecular Genetics, MS415 Medical Center, University of Kentucky, Lexington, KY 40536 0298, USA
    Microbes Infect 13:808-17. 2011
  4. ncbi request reprint Roles of the Yfe and Feo transporters of Yersinia pestis in iron uptake and intracellular growth
    Robert D Perry
    Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, 800 Rose St, MS415 Med Ctr, Lexington, KY 40536 0298, USA
    Biometals 20:699-703. 2007
  5. ncbi request reprint HmsP, a putative phosphodiesterase, and HmsT, a putative diguanylate cyclase, control Hms-dependent biofilm formation in Yersinia pestis
    Olga Kirillina
    Department of Microbiology, Immunology, and Molecular Genetics, MS415 Medical Center, University of Kentucky, Lexington, KY 40536 0298, USA
    Mol Microbiol 54:75-88. 2004
  6. pmc Polyamines are required for the expression of key Hms proteins important for Yersinia pestis biofilm formation
    Brian W Wortham
    Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY 40536, USA
    Environ Microbiol 12:2034-47. 2010
  7. doi request reprint Yersinia ironomics: comparison of iron transporters among Yersinia pestis biotypes and its nearest neighbor, Yersinia pseudotuberculosis
    Stanislav Forman
    Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY, USA
    Biometals 23:275-94. 2010
  8. ncbi request reprint Regulation of the Yersinia pestis Yfe and Ybt iron transport systems
    Robert D Perry
    Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY, USA
    Adv Exp Med Biol 529:275-83. 2003
  9. pmc Yersiniabactin production requires the thioesterase domain of HMWP2 and YbtD, a putative phosphopantetheinylate transferase
    Alexander G Bobrov
    Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington 40536 0084, USA
    Infect Immun 70:4204-14. 2002
  10. ncbi request reprint Identification of critical amino acid residues in the plague biofilm Hms proteins
    Stanislav Forman
    Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY 40536 0084, USA
    Microbiology 152:3399-410. 2006

Research Grants

  1. Yersiniabactin and plague pathogenesis
    ROBERT DONALD PERRY; Fiscal Year: 2011
  2. Characterization of the Hms phenotype of Yersinia pestis
    Robert Perry; Fiscal Year: 2003
  3. Characterization of the Hms phenotype of Yersinia pestis
    Robert Perry; Fiscal Year: 2007
  4. IRON TRANSPORT AND REGULATION IN YERSINIA PESTIS
    Robert Perry; Fiscal Year: 2007
  5. Yersiniabactin and plague pathogenesis
    Robert Perry; Fiscal Year: 2009
  6. IRON TRANSPORT AND REGULATION IN YERSINIA PESTIS
    Robert Perry; Fiscal Year: 2001
  7. HEMIN STORAGE AND UPTAKE IN YERSINIA PESTIS
    Robert Perry; Fiscal Year: 2001
  8. HEMIN STORAGE AND UPTAKE IN YERSINIA PESTIS
    Robert Perry; Fiscal Year: 1993
  9. Plague biofilm formation--regulation and function
    ROBERT DONALD PERRY; Fiscal Year: 2010
  10. Yersiniabactin and plague pathogenesis
    ROBERT DONALD PERRY; Fiscal Year: 2010

Collaborators

Detail Information

Publications32

  1. pmc Reduced synthesis of the Ybt siderophore or production of aberrant Ybt-like molecules activates transcription of yersiniabactin genes in Yersinia pestis
    M Clarke Miller
    Department of Chemistry, University of Kentucky, Lexington, KY 40506 0055, USA
    Microbiology 156:2226-38. 2010
    ..While these compounds are not biologically relevant to normal Ybt regulation, a comparison of the structures of Ybt and other signalling molecules will help in determining the chemical structures recognized as a Ybt signal...
  2. pmc Manganese transporters Yfe and MntH are Fur-regulated and important for the virulence of Yersinia pestis
    Robert D Perry
    Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY 40536 0298, USA
    Microbiology 158:804-15. 2012
    ..This suggests that Mn availability, bacterial Mn requirements or Mn transporters used by Y. pestis are different in the lungs (pneumonic plague) compared with systemic disease...
  3. pmc Yersiniabactin iron uptake: mechanisms and role in Yersinia pestis pathogenesis
    Robert D Perry
    Department of Microbiology, Immunology, and Molecular Genetics, MS415 Medical Center, University of Kentucky, Lexington, KY 40536 0298, USA
    Microbes Infect 13:808-17. 2011
    ..The Ybt system is essential for the ability of Yersinia pestis to cause bubonic plague and important in pneumonic plague as well. However, the ability to cause fatal septicemic plague is independent of Ybt...
  4. ncbi request reprint Roles of the Yfe and Feo transporters of Yersinia pestis in iron uptake and intracellular growth
    Robert D Perry
    Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, 800 Rose St, MS415 Med Ctr, Lexington, KY 40536 0298, USA
    Biometals 20:699-703. 2007
    ..1 cells. These results suggest that the Yfe and Feo systems are somewhat redundant ferrous iron transporters capable of iron acquisition during intracellular growth of the plague bacterium...
  5. ncbi request reprint HmsP, a putative phosphodiesterase, and HmsT, a putative diguanylate cyclase, control Hms-dependent biofilm formation in Yersinia pestis
    Olga Kirillina
    Department of Microbiology, Immunology, and Molecular Genetics, MS415 Medical Center, University of Kentucky, Lexington, KY 40536 0298, USA
    Mol Microbiol 54:75-88. 2004
    ..We propose that HmsT and HmsP together control the amount of biofilm produced in Y. pestis. Degradation of HmsT at 37 degrees C may be a critical factor in controlling the temperature-dependent expression of the Hms biofilm...
  6. pmc Polyamines are required for the expression of key Hms proteins important for Yersinia pestis biofilm formation
    Brian W Wortham
    Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY 40536, USA
    Environ Microbiol 12:2034-47. 2010
    ..Finally, we have shown that polyamines play a role in bubonic plague...
  7. doi request reprint Yersinia ironomics: comparison of iron transporters among Yersinia pestis biotypes and its nearest neighbor, Yersinia pseudotuberculosis
    Stanislav Forman
    Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY, USA
    Biometals 23:275-94. 2010
    ..pestis CO92 (epidemic orientalis biovar). Experimental studies failed to identify a role for hemin uptake systems in the virulence of pneumonic plague and suggest that Y. pestis CO92 does not make a siderophore other than Ybt...
  8. ncbi request reprint Regulation of the Yersinia pestis Yfe and Ybt iron transport systems
    Robert D Perry
    Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY, USA
    Adv Exp Med Biol 529:275-83. 2003
  9. pmc Yersiniabactin production requires the thioesterase domain of HMWP2 and YbtD, a putative phosphopantetheinylate transferase
    Alexander G Bobrov
    Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington 40536 0084, USA
    Infect Immun 70:4204-14. 2002
    ..In contrast, cells containing a single amino acid substitution (S2908A) in the terminal thioesterase domain of HMWP2 failed to exhibit any ybt regulatory defects but did not elaborate extracellular Ybt under iron-deficient conditions...
  10. ncbi request reprint Identification of critical amino acid residues in the plague biofilm Hms proteins
    Stanislav Forman
    Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY 40536 0084, USA
    Microbiology 152:3399-410. 2006
    ..Substitutions within the glycosyltransferase domain of HmsR and the deacetylase domain of HmsF abolished biofilm formation in Y. pestis. Surprisingly, substitution of highly conserved residues within COG1649 did not affect HmsF function...
  11. pmc Phenotypic convergence mediated by GGDEF-domain-containing proteins
    Roger Simm
    Department of Microbiology, Immunology, and Molecular Genetics, MS415 Medical Center, University of Kentucky, Lexington, KY 40536 0298, USA
    J Bacteriol 187:6816-23. 2005
    ..pestis cells carrying adrA under the control of an arabinose-inducible promoter produced substantial biofilms in the presence of arabinose. Finally, we demonstrate that HmsT is involved in the synthesis of cyclic di-GMP...
  12. ncbi request reprint Yersinia pestis lacZ expresses a beta-galactosidase with low enzymatic activity
    Alexander G Bobrov
    Department of Microbiology, Immunology, and Molecular Genetics, MS415 Medical Center, University of Kentucky, Lexington, 40536 0298, USA
    FEMS Microbiol Lett 255:43-51. 2006
    ..The Y. pestis lacZ promoter was not regulated by isopropylthiogalactoside or glucose. Finally, uptake of lactose by Y. pestis may be impaired...
  13. pmc Systematic analysis of cyclic di-GMP signalling enzymes and their role in biofilm formation and virulence in Yersinia pestis
    Alexander G Bobrov
    Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, KY, USA
    Mol Microbiol 79:533-51. 2011
    ..pestis from the ancestral Yersinia pseudotuberculosis was a significant reduction in the complexity of its c-di-GMP signalling network likely resulting from the different disease cycles of these human pathogens...
  14. pmc Yersinia pestis TonB: role in iron, heme, and hemoprotein utilization
    Robert D Perry
    Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, Kentucky 40536 8994, USA
    Infect Immun 71:4159-62. 2003
    ..Here we demonstrate that hemin uptake and iron utilization from Ybt are TonB dependent. However, the Yfe iron and manganese transport system does not require TonB...
  15. pmc Analysis of HmsH and its role in plague biofilm formation
    Arwa Abu Khweek
    Department of Microbiology, University of Kentucky, 800 Rose St, Lexington, KY, USA
    Microbiology 156:1424-38. 2010
    ..Finally, using a polar hmsH : : mini-kan mutant, we demonstrated that biofilm development is not important for the pathogenesis of bubonic or pneumonic plague in mice...
  16. pmc Temperature regulation of the hemin storage (Hms+) phenotype of Yersinia pestis is posttranscriptional
    Robert D Perry
    Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, Kentucky 40536, USA
    J Bacteriol 186:1638-47. 2004
    ..However, HmsT at 37 degrees C is sensitive to degradation by Lon and/or ClpPX. Thus, the stability of HmsH, HmsR, and HmsT proteins likely plays a role in temperature regulation of the Hms+ phenotype of Y. pestis...
  17. doi request reprint Insights into Yersinia pestis biofilm development: topology and co-interaction of Hms inner membrane proteins involved in exopolysaccharide production
    Alexander G Bobrov
    Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, Kentucky, USA
    Environ Microbiol 10:1419-32. 2008
    ..Biochemical analyses confirmed some of these protein-protein interactions. Our results indicate that synthesis and regulation of the Y. pestis biofilm EPS occurs in the cytoplasm by a proposed Hms enzymatic complex...
  18. ncbi request reprint Regulation of biofilm formation in Yersinia pestis
    Alexander G Bobrov
    Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, USA
    Adv Exp Med Biol 603:201-10. 2007
    ..Finally, protein-protein interactions and the cytoplasmic location of the enzymatic domains of HmsT and HmsP are evaluated...
  19. ncbi request reprint Functional quorum sensing systems affect biofilm formation and protein expression in Yersinia pestis
    Alexander G Bobrov
    Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, USA
    Adv Exp Med Biol 603:178-91. 2007
    ..Two-dimensional gel electrophoresis revealed altered levels of protein expression in a Y. pestis triple QS mutant at 26 degrees C and 37 degrees C...
  20. pmc Hierarchy of iron uptake systems: Yfu and Yiu are functional in Yersinia pestis
    Olga Kirillina
    Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY 40656 0298, USA
    Infect Immun 74:6171-8. 2006
    ..pestis and indicate that there is a hierarchy of iron transporters, with Ybt being most effective and Yiu being the least effective of those systems which have been characterized...
  21. ncbi request reprint The phosphodiesterase activity of the HmsP EAL domain is required for negative regulation of biofilm formation in Yersinia pestis
    Alexander G Bobrov
    Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, KY 40536 0298, USA
    FEMS Microbiol Lett 247:123-30. 2005
    ..While the proposed function of EAL-domain proteins is to linearize c-di-GMP, this is a direct demonstration of the required phosphodiesterase activity of a purified EAL-domain protein...
  22. ncbi request reprint Analysis of the aerobactin and ferric hydroxamate uptake systems of Yersinia pestis
    Stanislav Forman
    Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, KY 40536 0298, USA
    Microbiology 153:2332-41. 2007
    ..Mutations in the fhu operon, but not in iutA, affected the ability of KIM6 to use ferrichrome. This demonstrates that Y. pestis uses both ferrichrome and aerobactin, but has lost the ability to synthesize aerobactin...
  23. pmc The yersiniabactin transport system is critical for the pathogenesis of bubonic and pneumonic plague
    Jacqueline D Fetherston
    Department of Microbiology, Immunology, and Molecular Genetics, MS415 Medical Center, University of Kentucky, Lexington, KY 40536 0298, USA
    Infect Immun 78:2045-52. 2010
    ..Finally, a Delta pgm mutant had a greater loss of virulence than the Ybt biosynthetic mutant, indicating that the 102-kb pgm locus encodes a virulence factor, in addition to Ybt, that plays a role in the pathogenesis of pneumonic plague...
  24. pmc yadBC of Yersinia pestis, a new virulence determinant for bubonic plague
    Stanislav Forman
    Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY 40536 0298, USA
    Infect Immun 76:578-87. 2008
    ..pestis adherence, invasion, and virulence. We found that loss of yadBC caused a modest loss of invasiveness for epithelioid cells and a large decrease in virulence for bubonic plague but not for pneumonic plague in mice...
  25. doi request reprint Isolation and confirmation of Yersinia pestis mutants exempt from select agent regulations
    Robert D Perry
    Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, Kentucky, USA
    Curr Protoc Microbiol . 2008
    ..Strains lacking either the chromosomal pgm locus or the Lcr plasmid can be safely studied under BSL-2 conditions and are exempt from Select Agent regulations in the U.S...
  26. ncbi request reprint Crystal structure of ferric-yersiniabactin, a virulence factor of Yersinia pestis
    M Clarke Miller
    Department of Chemistry, University of Kentucky Lexington, Chemistry Physics Building, Room 104, Lexington, Kentucky 40506 0055, USA
    J Inorg Biochem 100:1495-500. 2006
    ..To our knowledge this is the first report of the ferric crystal structure of 5-member heterocycle siderophore...
  27. pmc Polyamines are essential for the formation of plague biofilm
    Chandra N Patel
    Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY 40536 0082, USA
    J Bacteriol 188:2355-63. 2006
    ..Chemical complementation of the double mutant and recovery of the biofilm defect were only observed with the polyamine putrescine...
  28. pmc Replication of Yersinia pestis in interferon gamma-activated macrophages requires ripA, a gene encoded in the pigmentation locus
    Céline Pujol
    Department of Molecular Genetics and Microbiology and Center for Infectious Diseases, State University of New York, Stony Brook, NY 11794 5222, USA
    Proc Natl Acad Sci U S A 102:12909-14. 2005
    ..These data demonstrate that intracellular Y. pestis can evade killing by macrophages that are exposed to IFN-gamma and identify a potential virulence gene encoded in the pgm locus that is required for this activity...
  29. ncbi request reprint Oral vaccination with different antigens from Yersinia pestis KIM delivered by live attenuated Salmonella typhimurium elicits a protective immune response against plague
    Christine G Branger
    Center for Infectious Diseases and Vaccinology, The Biodesign Institute, Arizona State University, Tempe, USA
    Adv Exp Med Biol 603:387-99. 2007
    ..This finding should facilitate the design and development of a new generation of vaccines against Y. pestis...
  30. doi request reprint Characterizing the dynamic nature of the Yersinia pestis periplasmic proteome in response to nutrient exhaustion and temperature change
    Rembert Pieper
    J Craig Venter Institute, Rockville, MD 20850, USA
    Proteomics 8:1442-58. 2008
    ..pestis life cycle were strongly altered in abundance. This included a putative nitrate/sulfonate/bicarbonate-specific SBP (Y1004), encoded by the virulence-associated plasmid pMT1 and increased in abundance at 37 degrees C...
  31. ncbi request reprint The Treponema pallidum tro operon encodes a multiple metal transporter, a zinc-dependent transcriptional repressor, and a semi-autonomously expressed phosphoglycerate mutase
    Karsten R O Hazlett
    Center for Microbial Pathogenesis, University of Connecticut Health Center, Farmington, Connecticut 06030 3710, USA
    J Biol Chem 278:20687-94. 2003
    ..Our data also indicate that Gpm expression and, therefore, glycolysis would not be abrogated when T. pallidum encounters high Zn2+ levels...
  32. pmc Genome sequence of Yersinia pestis KIM
    Wen Deng
    Laboratory of Genetics, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Bacteriol 184:4601-11. 2002
    ..In KIM-specific islands, new genes encode candidate pathogenicity proteins, including iron transport systems, putative adhesins, toxins, and fimbriae...

Research Grants32

  1. Yersiniabactin and plague pathogenesis
    ROBERT DONALD PERRY; Fiscal Year: 2011
    ..Understanding how the bacterium makes this siderophore and how its production is regulated may eventually lead to the development of either a vaccine or new drugs against plague and other bacterial diseases. ..
  2. Characterization of the Hms phenotype of Yersinia pestis
    Robert Perry; Fiscal Year: 2003
    ....
  3. Characterization of the Hms phenotype of Yersinia pestis
    Robert Perry; Fiscal Year: 2007
    ..abstract_text> ..
  4. IRON TRANSPORT AND REGULATION IN YERSINIA PESTIS
    Robert Perry; Fiscal Year: 2007
    ..Our studies will also provide insights into the role of Ybt and Yfe in the pathogenesis of plague and other disease-causing organisms and into the general importance of iron acquisition in bacterial disease processes. ..
  5. Yersiniabactin and plague pathogenesis
    Robert Perry; Fiscal Year: 2009
    ..Understanding how the bacterium makes this siderophore and how its production is regulated may eventually lead to the development of either a vaccine or new drugs against plague and other bacterial diseases. ..
  6. IRON TRANSPORT AND REGULATION IN YERSINIA PESTIS
    Robert Perry; Fiscal Year: 2001
    ....
  7. HEMIN STORAGE AND UPTAKE IN YERSINIA PESTIS
    Robert Perry; Fiscal Year: 2001
    ..The Hmu mechanism of hemin- compound utilization will be investigated and its role in the infectious process in mammals and fleas determined. ..
  8. HEMIN STORAGE AND UPTAKE IN YERSINIA PESTIS
    Robert Perry; Fiscal Year: 1993
    ..The Hmu mechanism of hemin- compound utilization will be investigated and its role in the infectious process in mammals and fleas determined...
  9. Plague biofilm formation--regulation and function
    ROBERT DONALD PERRY; Fiscal Year: 2010
    ..Understanding how the plague bacterium makes biofilm may eventually lead to ways to prevent biofilm formation in other bacterial pathogens. ..
  10. Yersiniabactin and plague pathogenesis
    ROBERT DONALD PERRY; Fiscal Year: 2010
    ..Understanding how the bacterium makes this siderophore and how its production is regulated may eventually lead to the development of either a vaccine or new drugs against plague and other bacterial diseases. ..
  11. Plague biofilm formation--regulation and function
    Robert Perry; Fiscal Year: 2009
    ..Understanding how the plague bacterium makes biofilm may eventually lead to ways to prevent biofilm formation in other bacterial pathogens. ..
  12. Characterization of the Hms phenotype of Yersinia pestis
    Robert Perry; Fiscal Year: 2006
    ..abstract_text> ..