Thomas T Perkins
Affiliation: University of Colorado
- Sequence-dependent pausing of single lambda exonuclease moleculesThomas T Perkins
Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
Science 301:1914-8. 2003..This sequence is found in the left lambda cohesive end, where exonuclease inhibition may contribute to the reduced recombination efficiency at that end...
- Forward and reverse motion of single RecBCD molecules on DNAThomas T Perkins
Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
Biophys J 86:1640-8. 2004..Our observations show that RecBCD-DNA complexes can exist in multiple, functionally distinct states that persist for many catalytic turnovers: such states may help tune enzyme activity for various biological functions...
- TFIIA changes the conformation of the DNA in TBP/TATA complexes and increases their kinetic stabilityAaron R Hieb
Department of Chemistry and Biochemistry, University of Colorado at Boulder, 215 UCB, Boulder, CO 80309 0215, USA
J Mol Biol 372:619-32. 2007..Furthermore, we present a refined model for the effect that TFIIA has on DNA conformation that takes into account potential changes in bend angle as well as twist angle...
- Elasticity of short DNA molecules: theory and experiment for contour lengths of 0.6-7 micromYeonee Seol
JILA, National Institute of Standards and Technology and University of Colorado, USA
Biophys J 93:4360-73. 2007....
- Measuring 0.1-nm motion in 1 ms in an optical microscope with differential back-focal-plane detectionLora Nugent-Glandorf
JILA, National Institute of Standards and Technology, Boulder, Colorado 80309 0440, USA
Opt Lett 29:2611-3. 2004..1 nm in 1 ms and stability of 0.5 nm over 60 s. Repeated steps of 0.4 nm at 1 Hz are resolved with a signal-to-noise ratio of 25...
- Gold nanoparticles: enhanced optical trapping and sensitivity coupled with significant heatingYeonee Seol
JILA, University of Colorado, and National Institute of Standards and Technology, Boulder, CO 80309, USA
Opt Lett 31:2429-31. 2006..Also, such particles, with their increased detection sensitivity, make excellent probes for certain zero-force biophysical assays...
- Stabilization of an optical microscope to 0.1 nm in three dimensionsAshley R Carter
JILA, University of Colorado and National Institute of Standards and Technology, Boulder, CO 80309, USA
Appl Opt 46:421-7. 2007..11, 0.10, and 0.09 nm (rms) and long-term (100 s) stabilities of 0.17, 0.12, and 0.35 nm (rms) in x, y, and z, respectively, as measured by an independent detection laser...