A Periasamy

Summary

Affiliation: University of Virginia
Country: USA

Publications

  1. ncbi request reprint An evaluation of two-photon excitation versus confocal and digital deconvolution fluorescence microscopy imaging in Xenopus morphogenesis
    A Periasamy
    W M Keck Center for Cellular Imaging, Gilmer Hall, University of Virginia, Charlottesville, Virginia 22903, USA
    Microsc Res Tech 47:172-81. 1999
  2. ncbi request reprint Fluorescence resonance energy transfer microscopy: a mini review
    A Periasamy
    University of Virginia, W M Keck Center for Cellular Imaging, Department of Biology, Gilmer Hall, Charlottesville, Virginia 22904, USA
    J Biomed Opt 6:287-91. 2001
  3. ncbi request reprint Intensity range based quantitative FRET data analysis to localize protein molecules in live cell nuclei
    Ye Chen
    W M Keck Center for Cellular Imaging, University of Virginia, Gilmer Hall, Charlottesville, Virginia 22904, USA
    J Fluoresc 16:95-104. 2006
  4. ncbi request reprint Fluorescence resonance energy transfer microscopy of localized protein interactions in the living cell nucleus
    R N Day
    Department of Medicine, NSF Center for Biological Timing, Charlottesville, Virginia 22908, USA
    Methods 25:4-18. 2001
  5. pmc Characterization of an improved donor fluorescent protein for Forster resonance energy transfer microscopy
    Richard N Day
    University of Virginia Health System, Departments of Medicine and Cell Biology, P O Box 800578, Charlottesville, Virginia 22908 0578, USA
    J Biomed Opt 13:031203. 2008
  6. ncbi request reprint Localization of protein-protein interactions in live cells using confocal and spectral imaging FRET microscopy
    Ye Chen
    Department of Biology, W M Keck Center for Cellular Imaging, Gilmer Hall, University of Virginia, Charlottesville, VA 22904, USA
    Indian J Exp Biol 45:48-57. 2007
  7. ncbi request reprint Nitric-oxide-induced depolarization of neuronal mitochondria: implications for neuronal cell death
    Nina J Solenski
    Department of Neurology, University of Virginia Health Sciences System, Charlottesville, VA 22908, USA
    Mol Cell Neurosci 24:1151-69. 2003
  8. pmc Imaging the localized protein interactions between Pit-1 and the CCAAT/enhancer binding protein alpha in the living pituitary cell nucleus
    Richard N Day
    Department of Medicine, University of Virginia Health Sciences Center, Charlottesville, Virginia, 22908, USA
    Mol Endocrinol 17:333-45. 2003
  9. pmc Characterization of an orange acceptor fluorescent protein for sensitized spectral fluorescence resonance energy transfer microscopy using a white-light laser
    Yuansheng Sun
    University of Virginia, Department of Biology, W M Keck Center for Cellular Imaging, McCormick Road, Charlottesville, Virginia 22904, USA
    J Biomed Opt 14:054009. 2009
  10. pmc Three-color spectral FRET microscopy localizes three interacting proteins in living cells
    Yuansheng Sun
    W M Keck Center for Cellular Imaging, Departments of Biology and Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA
    Biophys J 99:1274-83. 2010

Research Grants

  1. Multiphoton Spectral Imaging Microscopy
    Ammasi Periasamy; Fiscal Year: 2005

Collaborators

Detail Information

Publications33

  1. ncbi request reprint An evaluation of two-photon excitation versus confocal and digital deconvolution fluorescence microscopy imaging in Xenopus morphogenesis
    A Periasamy
    W M Keck Center for Cellular Imaging, Gilmer Hall, University of Virginia, Charlottesville, Virginia 22903, USA
    Microsc Res Tech 47:172-81. 1999
    ..Also, we have demonstrated that the quality of the image changes at different depths for various excitation powers...
  2. ncbi request reprint Fluorescence resonance energy transfer microscopy: a mini review
    A Periasamy
    University of Virginia, W M Keck Center for Cellular Imaging, Department of Biology, Gilmer Hall, Charlottesville, Virginia 22904, USA
    J Biomed Opt 6:287-91. 2001
    ..This paper will provide a brief review of the above-mentioned FRET techniques...
  3. ncbi request reprint Intensity range based quantitative FRET data analysis to localize protein molecules in live cell nuclei
    Ye Chen
    W M Keck Center for Cellular Imaging, University of Virginia, Gilmer Hall, Charlottesville, Virginia 22904, USA
    J Fluoresc 16:95-104. 2006
    ....
  4. ncbi request reprint Fluorescence resonance energy transfer microscopy of localized protein interactions in the living cell nucleus
    R N Day
    Department of Medicine, NSF Center for Biological Timing, Charlottesville, Virginia 22908, USA
    Methods 25:4-18. 2001
    ..The use of different-color fluorescent proteins in combination with FRET offers the opportunity to study the complex behavior of key regulatory proteins in their natural environment within the living cell...
  5. pmc Characterization of an improved donor fluorescent protein for Forster resonance energy transfer microscopy
    Richard N Day
    University of Virginia Health System, Departments of Medicine and Cell Biology, P O Box 800578, Charlottesville, Virginia 22908 0578, USA
    J Biomed Opt 13:031203. 2008
    ....
  6. ncbi request reprint Localization of protein-protein interactions in live cells using confocal and spectral imaging FRET microscopy
    Ye Chen
    Department of Biology, W M Keck Center for Cellular Imaging, Gilmer Hall, University of Virginia, Charlottesville, VA 22904, USA
    Indian J Exp Biol 45:48-57. 2007
    ..Also outline are issues involved in protein imaging using light microscopy techniques and the advantages of lifetime imaging of protein-protein interactions...
  7. ncbi request reprint Nitric-oxide-induced depolarization of neuronal mitochondria: implications for neuronal cell death
    Nina J Solenski
    Department of Neurology, University of Virginia Health Sciences System, Charlottesville, VA 22908, USA
    Mol Cell Neurosci 24:1151-69. 2003
    ..These data suggest that NO-induced NMDA receptor activation is closely linked to intramitochondrial NO-peroxynitrite/RNS formation and thereby acts as a major mediator of neuronal cell death...
  8. pmc Imaging the localized protein interactions between Pit-1 and the CCAAT/enhancer binding protein alpha in the living pituitary cell nucleus
    Richard N Day
    Department of Medicine, University of Virginia Health Sciences Center, Charlottesville, Virginia, 22908, USA
    Mol Endocrinol 17:333-45. 2003
    ..The intimate association of Pit-1 and C/EBPalpha at certain sites within the living cell nucleus could foster their combinatorial activities in the regulation of pituitary-specific gene expression...
  9. pmc Characterization of an orange acceptor fluorescent protein for sensitized spectral fluorescence resonance energy transfer microscopy using a white-light laser
    Yuansheng Sun
    University of Virginia, Department of Biology, W M Keck Center for Cellular Imaging, McCormick Road, Charlottesville, Virginia 22904, USA
    J Biomed Opt 14:054009. 2009
    ..The white-light laser generally extends the usage of orange and red/far-red FPs in sensitized FRET microscopy assays by tailoring excitation and emission precisely to the needs of the FRET pair...
  10. pmc Three-color spectral FRET microscopy localizes three interacting proteins in living cells
    Yuansheng Sun
    W M Keck Center for Cellular Imaging, Departments of Biology and Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA
    Biophys J 99:1274-83. 2010
    ..We show how the 3sFRET microscopy method represents a promising live-cell imaging technique to monitor the interactions between three labeled cellular components...
  11. pmc Monitoring dynamic protein interactions with photoquenching FRET
    Ignacio A Demarco
    Department of Medicine, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA
    Nat Methods 3:519-24. 2006
    ....
  12. pmc Characterization of spectral FRET imaging microscopy for monitoring nuclear protein interactions
    Ye Chen
    W M Keck Center for Cellular Imaging, Departments of Biology and Biomedical Engineering, Gilmer Hall, University of Virginia, Charlottesville, VA 22904, USA
    J Microsc 228:139-52. 2007
    ..The method is then used to detect the homo-dimerization of a transcription factor in the nucleus of living cells, and then to measure the interactions of that protein with a second transcription factor...
  13. pmc Additional correction for energy transfer efficiency calculation in filter-based Forster resonance energy transfer microscopy for more accurate results
    Yuansheng Sun
    University of Virginia, W M Keck Center for Cellular Imaging, Department of Biology and Department of Biomedical Engineering, Charlottesville, Virginia 22904, USA
    J Biomed Opt 15:020513. 2010
    ....
  14. pmc Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations
    Rajesh Babu Sekar
    W M Keck Center for Cellular Imaging, Department of Biology, Gilmer Hall 064, University of Virginia, Charlottesville, VA 22904, USA
    J Cell Biol 160:629-33. 2003
    ....
  15. ncbi request reprint Truncated estrogen receptor product-1 suppresses estrogen receptor transactivation by dimerization with estrogen receptors alpha and beta
    E M Resnick
    Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22903, USA
    J Biol Chem 275:7158-66. 2000
    ..Therefore, TERP-1 suppression of ER transcription occurs primarily by formation of inactive heterodimers and secondarily by competition for coactivators...
  16. ncbi request reprint Potassium ion induced changes of crystal structure and fluorescence of a crown ether
    Qiu Xian Ren
    Department of Chemistry, University of Virginia, Charlottesville, VA 22904 4319, USA
    J Fluoresc 17:249-55. 2007
    ..In CEAK, disruption of the pi-pi stacking structure forces a large separation between the anthracene rings, which yields an anthracene monomer emission. Luminescence lifetime data support the assignments...
  17. ncbi request reprint Nanosecond fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy to localize the protein interactions in a single living cell
    M Elangovan
    W M Keck Center for Cellular Imaging, Department of Biology, Gilmer Hall, University of Virginia, Charlottesville, VA 22904, USA
    J Microsc 205:3-14. 2002
    ..The one- and two-component analysis of the donor molecule lifetime in the presence of acceptor demonstrates the distance distribution between interacting proteins...
  18. ncbi request reprint Angiotensin II type 2 receptor-bradykinin B2 receptor functional heterodimerization
    Peter M Abadir
    Division of Endocrinology and Metabolism, Department of Medicine, University of Virginia, Charlottesville, VA, USA
    Hypertension 48:316-22. 2006
    ..Controlling the expression of AT2R-B2R, consequently influencing their biologically active dimerization, presents a potential therapeutic target for the treatment of hypertension and other cardiovascular and renal disorders...
  19. pmc Confocal FRET microscopy to measure clustering of ligand-receptor complexes in endocytic membranes
    Horst Wallrabe
    Department of Biology, University of Virginia, Charlottesville, Virginia 22904, USA
    Biophys J 85:559-71. 2003
    ..Here, we present a new sensitive FRET-based method to quantify the co-localization and distribution of ligand-receptor complexes in apical endocytic membranes of polarized cells...
  20. ncbi request reprint Imaging protein molecules using FRET and FLIM microscopy
    Horst Wallrabe
    Keck Center for Cellular Imaging, Department of Biology, University of Virginia, Gilmer Hall, Charlottesville, Virginia 22904, USA
    Curr Opin Biotechnol 16:19-27. 2005
    ..g. clustering), in the study of conformational changes, in the analysis of binding sequences, and in applications such as high-throughput screening...
  21. ncbi request reprint Fluorescence lifetime imaging (FLIM) of green fluorescent fusion proteins in living cells
    Ammasi Periasamy
    Department of Biology, W M Keck Center for Cellular Imaging, University of Virginia, Charlottesville, VA, USA
    Methods Mol Biol 183:89-100. 2002
  22. ncbi request reprint Characterization of two-photon excitation fluorescence lifetime imaging microscopy for protein localization
    Ye Chen
    W M Keck Center for Cellular Imaging, University of Virginia, Charlottesville, Virginia 22904, USA
    Microsc Res Tech 63:72-80. 2004
    ..We describe the development and characterization of the 2P-FRET-FLIM imaging system with the Bio-Rad Radiance2100 confocal/multiphoton microscopy system...
  23. ncbi request reprint One- and two-photon fluorescence resonance energy transfer microscopy to establish a clustered distribution of receptor-ligand complexes in endocytic membranes
    Horst Wallrabe
    University of Virginia, Department of Biology, Gilmer Hall, 057, Charlottesville, Virginia 22904, USA
    J Biomed Opt 8:339-46. 2003
    ..One- and two-photon FRET microscopy assays show that polymeric IgA-receptor-ligand complexes are organized in clusters within apical endocytic membranes of polarized Madin-Darby canine kidney cells...
  24. ncbi request reprint Dynamic imaging using fluorescence resonance energy transfer
    Masilamani Elangovan
    University of Virginia, Charlottesville 22904, USA
    Biotechniques 32:1260-2, 1264-5. 2002
  25. doi request reprint Chapter 22: Quantitation of protein-protein interactions: confocal FRET microscopy
    Ammasi Periasamy
    University of Virginia, W M Keck Center for Cellular Imaging, Department of Biology, Charlottesville, Virginia 22904, USA
    Methods Cell Biol 89:569-98. 2008
    ..The assays described are applicable to many other biological applications...
  26. ncbi request reprint Illuminating protein interactions in tissue using confocal and two-photon excitation fluorescent resonance energy transfer microscopy
    James D Mills
    University of Virginia Health Sciences Center, Department of Neurosurgery, Charlottesville, Virginia 22908, USA
    J Biomed Opt 8:347-56. 2003
    ..Further, we report on a method to reliably detect protein interactions within aldehyde fixed tissue sections through conventional immunohistochemical approaches...
  27. ncbi request reprint Issues in confocal microscopy for quantitative FRET analysis
    Horst Wallrabe
    Keck Center for Cellular Imaging, Department of Biology, Gilmer Hall, University of Virginia, Charlottesville, Virginia 22903, USA
    Microsc Res Tech 69:196-206. 2006
    ..Our results indicating a clustered organization of TFR-Tfn complexes fit the well-known homodimeric structure of TFR. These quantitative approaches can be adapted for other biological applications of FRET...
  28. ncbi request reprint Characterization of one- and two-photon excitation fluorescence resonance energy transfer microscopy
    Masilamani Elangovan
    W M Keck Center for Cellular Imaging, Gilmer Hall, 22904, Charlottesville, VA, USA
    Methods 29:58-73. 2003
    ..For these proteins, the results revealed that the extent of correction affects the conventionally calculated energy transfer efficiency (E) and the distance (r) between donor and acceptor molecules by 38 and 9%, respectively...
  29. doi request reprint Visible fluorescent proteins
    Ammasi Periasamy
    J Biomed Opt 13:031201. 2008
  30. ncbi request reprint Protein localization in living cells and tissues using FRET and FLIM
    Ye Chen
    W M Keck Center for Cellular Imaging University of Virginia Charlottesville, VA 22904, USA
    Differentiation 71:528-41. 2003
    ..In this paper, we describe various FRET microscopy techniques and its application to protein-protein interactions...
  31. ncbi request reprint Multiphoton microscopy
    Ammasi Periasamy
    J Biomed Opt 8:327-8. 2003
  32. ncbi request reprint Heterooligomerization between vasotocin and corticotropin-releasing hormone (CRH) receptors augments CRH-stimulated 3',5'-cyclic adenosine monophosphate production
    Marina V Mikhailova
    Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, 4301 West Markham Street, Slot 750, Little Rock, Arkansas 72205, USA
    Mol Endocrinol 21:2178-88. 2007
    ....
  33. pmc Rab4 and Rab11 coordinately regulate the recycling of angiotensin II type I receptor as demonstrated by fluorescence resonance energy transfer microscopy
    Hewang Li
    Georgetown University Medical Center, Department of Pediatrics, Washington, DC 20057, USA
    J Biomed Opt 13:031206. 2008
    ..Co-immunoprecipitation data confirmed these dynamic associations, which were disrupted by silencing of either the Rab4 or Rab11 gene. Based on these observations, we propose a Rab4 and Rab11 coordinated model for AT1R recycling...

Research Grants1

  1. Multiphoton Spectral Imaging Microscopy
    Ammasi Periasamy; Fiscal Year: 2005
    ..abstract_text> ..