Research Topics
Genomes and Genes | Camillo PeracchiaSummaryAffiliation: University of Rochester Country: USA Publications
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Publications
Chemical gating of gap junction channelsC Peracchia
Department of Pharmacology and Physiology, University of Rochester, School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, New York 14642 8711, USA
Methods 20:188-95. 2000..In addition, various channel forms, including homotypic, heterotypic, and heteromeric channel combinations, have been evaluated for chemical gating sensitivity...- Unusual slow gating of gap junction channels in oocytes expressing connexin32 or its COOH-terminus truncated mutantCamillo Peracchia
Department of Pharmacology and Physiology, University of Rochester School of Medicine, 601 Elmwood Avenue, Rochester, NY 14642 8711, USA
J Membr Biol 215:161-8. 2007..Based on previous evidence for direct calmodulin (CaM) involvement in chemical/slow gating, this may also be CaM-mediated... - Inversion of both gating polarity and CO2 sensitivity of voltage gating with D3N mutation of Cx50Camillo Peracchia
Department of Pharmacology and Physiology, University of Rochester School of Medicine, 601 Elmwood Ave, Rochester, NY 14642 8711, USA
Am J Physiol Cell Physiol 288:C1381-9. 2005..This hypothesis is confirmed here by evidence that the D3N mutation inverts the gating polarity as well as the effect of CO(2) on V(j) gating sensitivity and speed... - CO(2) sensitivity of voltage gating and gating polarity of gapjunction channels--connexin40 and its COOH-terminus-truncated mutantC Peracchia
Department of Pharmacology and Physiology, University of Rochester School of Medicine, 601 Elmwood Ave, Rochester, NY 14642 8711, USA
J Membr Biol 200:105-13. 2004.... - Chemical gating of gap junction channels; roles of calcium, pH and calmodulinCamillo Peracchia
Department of Pharmacology and Physiology, University of Rochester, School of Medicine and Dentistry, Rochester, NY 14642 8711, USA
Biochim Biophys Acta 1662:61-80. 2004..At least three molecular models of channel gating have been proposed, but all of them are mostly based on circumstantial evidence... - The voltage gates of connexin channels are sensitive to CO(2)Camillo Peracchia
Department of Pharmacology and Physiology, University of Rochester, Rochester, NY 14642, USA
Cell Commun Adhes 10:233-7. 2003..In contrast, sensitivity and speed of V(j) gating of Cx40 and Cx26 channels decreased, rather than increased, with CO(2) application... - Is the voltage gate of connexins CO2-sensitive? Cx45 channels and inhibition of calmodulin expressionC Peracchia
University of Rochester, School of Medicine and Dentistry, Rochester, NY 14642 8711, USA
J Membr Biol 195:53-62. 2003..The data indicate that both the speed and sensitivity of V(j)-dependent inactivation of the junctional current of Cx45 channels are affected by CO2 application, and that CaM plays a key role in channel gating... - Interplay between cystic fibrosis transmembrane regulator and gap junction channels made of connexins 45, 40, 32 and 50 expressed in oocytesBasilio A Kotsias
Department of Pharmacology and Physiology, University of Rochester School of Medicine, 601 Elmwood Avenue, Rochester, NY 14642 8711, USA
J Membr Biol 214:1-8. 2006..The data suggest a cross-talk between CFTR and a variety of gap junction channels. Cytoskeletal scaffolding proteins and/or other intermediate cytoplasmic proteins are likely to play a role in CFTR-Cx interaction... - Interactions of connexins with other membrane channels and transportersMarc Chanson
Department of Pediatrics, Geneva University Hospitals, Geneva, Switzerland
Prog Biophys Mol Biol 94:233-44. 2007..Although the precise molecular nature of these interactions has yet to be defined, their consequences may be critical for normal tissue homeostasis... - Calmodulin association with connexin32-derived peptides suggests trans-domain interaction in chemical gating of gap junction channelsRyan Dodd
Division of Basic Medical Sciences, St George s, University of London, London, SW17 0RE United Kingdom
J Biol Chem 283:26911-20. 2008..Our data, showing separate functions of the N- and C-lobes of calmodulin in the interactions with connexin32, suggest trans-domain or trans-subunit bridging by calmodulin as a possible mechanism of gap junction gating...
Research Grants
- STRUCTURE/FUNCTION IN LOW RESISTANCE JUNCTIONSCamillo Peracchia; Fiscal Year: 2000..Finally, the molecular basis of channel insulation and connexin interaction will be probed through appropriate deletions and mutations of external Cx domains. ..
- STRUCTURE & FUNCTION IN LOW RESISTANCE JUNCTIONSCamillo Peracchia; Fiscal Year: 1980..Various methodologies including thin sectioning, membrane fractionation, SDS PAGE, intracellular recordings etc. will be applied. ..
- STRUCTURE-FUNCTION IN LOW RESISTANCE JUNCTIONSCamillo Peracchia; Fiscal Year: 1992..The extent of gap junction phosphorylation in coupled/uncoupled state will be determined in normal and RSV-transformed cells. The nature of interparticle bridges will be determined in split junctions...
- STRUCTURE-FUNCTION IN LOW-RESISTANCE JUNCTIONSCamillo Peracchia; Fiscal Year: 1993..The hypothesis suggesting a mostly hydrophobic connexin domain as part of the external "channel seal" will be tested by expressing appropriate connexins mutants...
- Structure Function in Low Resistance JunctionsCamillo Peracchia; Fiscal Year: 2005..The relevance of a potential CaM binding site identified in connexins (similar to that of CaM kinase II) will be tested with deletion and mutation of crucial Cx32 residues. ..