Research Topics
Genomes and Genes | John PelusoSummaryAffiliation: University of Connecticut Health Center Country: USA Publications
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Publications
Plasminogen activator inhibitor 1 RNA-binding protein interacts with progesterone receptor membrane component 1 to regulate progesterone's ability to maintain the viability of spontaneously immortalized granulosa cells and rat granulosa cellsJohn J Peluso
Department of Cell Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
Biol Reprod 88:20. 2013..Surprisingly, PGRMC1-PAIRBP1 interaction is not required for P4 binding or the cellular localization of PGRMC1 but rather appears to couple PGRMC1 to downstream components of the P4-PGRMC1 signal transduction pathway...
Evidence for a genomic mechanism of action for progesterone receptor membrane component-1John J Peluso
Department of Cell Biology, University of CT Health Center, Farmington, CT 06030, United States
Steroids 77:1007-12. 2012....- Progesterone regulation of progesterone receptor membrane component 1 (PGRMC1) sumoylation and transcriptional activity in spontaneously immortalized granulosa cellsJohn J Peluso
Department of Cell Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
Endocrinology 153:3929-39. 2012.... 
Progesterone membrane receptor component 1 expression in the immature rat ovary and its role in mediating progesterone's antiapoptotic actionJ J Peluso
Department of Cell Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
Endocrinology 147:3133-40. 2006..At the plasma membrane, these two proteins interact to form a complex that is required for P4 to transduce its antiapoptotic action...- N-cadherin mediated cell contact inhibits germinal vesicle breakdown in mouse oocytes maintained in vitroJ J Peluso
Cell Biology and Obstetrics and Gynecology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
Reproduction 131:429-37. 2006..The involvement of FGF receptors is supported by the observations that FGF and a N-cadherin peptide known to activate FGF receptors inhibit GVBD... - Progesterone maintains basal intracellular adenosine triphosphate levels and viability of spontaneously immortalized granulosa cells by promoting an interaction between 14-3-3sigma and ATP synthase beta/precursor through a protein kinase G-dependent mechaJohn J Peluso
Department of Physiology, University of Connecticut Health Center, Farmington, Connecticut 06030 1230, USA
Endocrinology 148:2037-44. 2007.... - Non-genomic actions of progesterone in the normal and neoplastic mammalian ovaryJohn J Peluso
Department of Cell Biology, University of Connecticut School of Medicine, Farmington, Connecticut 06030, USA
Semin Reprod Med 25:198-207. 2007.... - Progesterone receptor membrane component-1 (PGRMC1) is the mediator of progesterone's antiapoptotic action in spontaneously immortalized granulosa cells as revealed by PGRMC1 small interfering ribonucleic acid treatment and functional analysis of PGRMC1 mJohn J Peluso
Department of Cell Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
Endocrinology 149:534-43. 2008..Additional studies are required to more precisely delineate the role of each PGRMC1 domain in transducing P4's antiapoptotic action... - Regulation of ovarian cancer cell viability and sensitivity to cisplatin by progesterone receptor membrane component-1John J Peluso
Department of Cell Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
J Clin Endocrinol Metab 93:1592-9. 2008..Progesterone (P4) influences ovarian cancer cells by an unknown mechanism... - Progesterone activates a progesterone receptor membrane component 1-dependent mechanism that promotes human granulosa/luteal cell survival but not progesterone secretionJohn J Peluso
Department of Cell Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
J Clin Endocrinol Metab 94:2644-9. 2009..Progesterone (P4) promotes its own secretion and the survival of human granulosa/luteal (GL) cells... - Progesterone receptor membrane component-1 regulates the development and Cisplatin sensitivity of human ovarian tumors in athymic nude miceJohn J Peluso
Department of Cell Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
Endocrinology 150:4846-54. 2009..Taken together, these findings indicate that PGRMC1 plays an essential role in the development and CDDP sensitivity of human ovarian tumors... - Progesterone inhibits apoptosis in part by PGRMC1-regulated gene expressionJ J Peluso
Department of Cell Biology, University of Connecticut Health Center, Farmington, CT 06030, USA
Mol Cell Endocrinol 320:153-61. 2010..Taken together, the present studies suggest a genomic component to PGRMC1's anti-apoptotic mechanism of action, which requires the presence of the PGRMC1 dimer... - Progesterone signaling mediated through progesterone receptor membrane component-1 in ovarian cells with special emphasis on ovarian cancerJohn J Peluso
Department of Cell Biology, University of CT Health Center, Farmington, CT 06030, USA
Steroids 76:903-9. 2011..How PGRMC1 regulates cell viability and in turn tumor growth is not known but part of the mechanism likely involves the regulation of genes that promote cell survival and inhibit apoptosis... - Multiplicity of progesterone's actions and receptors in the mammalian ovaryJohn J Peluso
Department of Cell Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
Biol Reprod 75:2-8. 2006.... - Expression and function of PAIRBP1 within gonadotropin-primed immature rat ovaries: PAIRBP1 regulation of granulosa and luteal cell viabilityJohn J Peluso
Department of Cell Biology, University of Conneticut Health Center, Farmington, Connecticut 06030, USA
Biol Reprod 73:261-70. 2005..The exact mechanism of PAIRBP1's action remains to be elucidated, but it may involve an interaction with PGRMC1... - Rapid actions of progesterone on granulosa cellsJohn J Peluso
Department of Physiology and Obstetrics and Gynecology, University of CT Health Center, 263 Farmington Ave, Farmington, CT 06030, USA
Steroids 69:579-83. 2004..A nearly identical unnamed protein was found in a cDNA library of mouse lung (Accession number: AK004678). Whether RDA288 functions as a membrane receptor for P4 remains to be determined... - Progesterone maintains large rat granulosa cell viability indirectly by stimulating small granulosa cells to synthesize basic fibroblast growth factorJ J Peluso
Department of Obstetrics and Gynecology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
Biol Reprod 60:290-6. 1999..Basic FGF then activates its receptors within large GCs, and this initiates a signal transduction pathway that maintains large GC viability... - N-cadherin-mediated cell contact regulates ovarian surface epithelial cell survivalJ J Peluso
Departments of Physiology and Obstetrics and Gynecology, University of Connecticut Health Center, Farmington 06030, USA
Biol Signals Recept 9:115-21. 2000..This hypothesis requires further testing and validation... - Effect of disrupting cell contact on the nuclear accumulation of beta-catenin and subsequent apoptosis of rat ovarian surface epithelial cells in vitroJ J Peluso
Department of Physiology, University of Connecticut Health Center, Farmington, CT 06030, USA
Endocrine 12:295-302. 2000..If calcium-dependent cell contacts are broken, beta-catenin accumulates within the nucleus, where it promotes transcription and ultimately the apoptotic death of ROSE-179 cells... - E-cadherin-mediated cell contact prevents apoptosis of spontaneously immortalized granulosa cells by regulating Akt kinase activityJ J Peluso
Departments of Physiology and Obstetrics and Gynecology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
Biol Reprod 64:1183-90. 2001.... - Characterization of a putative membrane receptor for progesterone in rat granulosa cellsJ J Peluso
Departments of Physiology and Obstetrics and Gynecology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
Biol Reprod 65:94-101. 2001..These studies taken together support the concept that the 60-kDa P(4) binding protein functions as a low-affinity, high-capacity membrane receptor for P(4)... - Basic fibroblast growth factor maintains calcium homeostasis and granulosa cell viability by stimulating calcium efflux via a PKC delta-dependent pathwayJ J Peluso
Departments of Physiology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
Endocrinology 142:4203-11. 2001.... - Progesterone mediates its anti-mitogenic and anti-apoptotic actions in rat granulosa cells through a progesterone-binding protein with gamma aminobutyric acidA receptor-like featuresJ J Peluso
Department of Obstetrics and Gynecology, University of Connecticut Health Center, Farmington 06030, USA
Biol Reprod 58:1131-7. 1998..Collectively, these studies suggest that P4 mediates its anti-mitotic and anti-apoptotic effects through this 60-kDa P4BP, which has GABA(A) receptor-like properties and is localized within the surface membrane of GCs... - Involvement of an unnamed protein, RDA288, in the mechanism through which progesterone mediates its antiapoptotic action in spontaneously immortalized granulosa cellsJ J Peluso
Department of Cell Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
Endocrinology 145:3014-22. 2004..Finally, pretreatment with the RDA288 antibody blocked P4's antiapoptotic actions. Taken together, these data suggest that RDA288 plays a significant role in mediating P4's antiapoptotic action in granulosa cells... - Membrane-initiated events account for progesterone's ability to regulate intracellular free calcium levels and inhibit rat granulosa cell mitosisJohn J Peluso
Department of Physiology, University of Connecticut Health Center, Farmington, CT 06030, USA
Biol Reprod 67:379-85. 2002..This antibody was also used in immunocytochemical studies to detect a protein that was associated with the plasma membrane of SIGCs. It is proposed that this 60-kDa protein mediates P(4)'s membrane-initiated actions... - Expression pattern and role of a 60-kilodalton progesterone binding protein in regulating granulosa cell apoptosis: involvement of the mitogen-activated protein kinase cascadeJ J Peluso
Department of Physiology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
Biol Reprod 68:122-8. 2003.... - Progesterone as a regulator of granulosa cell viabilityJ J Peluso
Department of Physiology, University of Connecticut Health Center, Farmington, CT 06030, USA
J Steroid Biochem Mol Biol 85:167-73. 2003..These studies support the concept that the 60kDa P4 binding protein functions as membrane receptor for P4 which activates a PKG-dependent mechanism to regulate granulosa cell survival... - Progesterone regulation of human granulosa/luteal cell viability by an RU486-independent mechanismLawrence Engmann
Department of Obstetrics and Gynecology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
J Clin Endocrinol Metab 91:4962-8. 2006.... - In vitro viability and secretory capacity of human luteinized granulosa cells after gonadotropin-releasing hormone agonist trigger of oocyte maturationLawrence Engmann
Center for Advanced Reproductive Services, University of Connecticut Health Center, Dowling South Building, 263 Farmington Avenue, Farmington, CT 06030 6224, USA
Fertil Steril 96:198-202. 2011.... - Murine cytomegalovirus influences Foxj1 expression, ciliogenesis, and mucus plugging in mice with allergic airway diseaseCarol A Wu
Department of Immunology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 1319, USA
Am J Pathol 172:714-24. 2008..Together, these findings suggest that MCMV infection of the airway epithelium enhances goblet cell metaplasia and diminishes efficient mucociliary clearance in mice with AAD, resulting in increased mucus plugging... - Basic fibroblast growth factor (bFGF) regulation of the plasma membrane calcium ATPase (PMCA) as part of an anti-apoptotic mechanism of actionJohn J Peluso
Department of Physiology, University of Connecticut Health Center, Farmington, CT 06030, USA
Biochem Pharmacol 66:1363-9. 2003..The ability of bFGF to stimulate calcium efflux involved the plasma membrane calcium ATPase (PMCA). Interestingly, bFGF-activated PKCdelta appeared to regulate PMCA activity in part by promoting its membrane localization... - Bronchial epithelial cells produce IL-5: implications for local immune responses in the airwaysCarol A Wu
Department of Immunology, University of Connecticut Health Center, Farmington, CT 06030 1319, USA
Cell Immunol 264:32-41. 2010..Thus, IL-5 production by BEC can impact the microenvironment of the lung, modifying pathologic and protective immune responses in the airways... - Reciprocal regulation by estradiol 17-beta of ezrin and cadherin-catenin complexes in pituitary GH3 cellsPerry M Smith
Department of Physiology, University of Connecticut Health Center, Farmington 06030, USA
Endocrine 17:219-28. 2002..These findings indicate that E2 enriches the cell membrane with ezrin-membrane protein complexes by both increasing ezrin expression and by enlarging the relative area of nonadhesive membrane to which ezrin is targeted... - Progesterone receptor membrane component 1--many tasks for a versatile proteinRalf M Losel
Clinical Pharmacology Mannheim, University of Heidelberg, Mannheim, Germany
Steroids 73:929-34. 2008..Here, we summarize a few sometimes neglected pieces of evidence from the literature along with unpublished findings on the properties and functions of PGRMC1... - Expression of progesterone receptor membrane component 1 and its partner serpine 1 mRNA binding protein in uterine and placental tissues of the mouse and humanLing Zhang
Vincent Center for Reproductive Biology, Harvard Medical School, Boston, MA 02114, USA
Mol Cell Endocrinol 287:81-9. 2008..These studies provide a platform from which to build a clearer understanding of P(4) actions in the female reproductive tract and placental tissues that are mediated by non-classical mechanisms...
Research Grants
- Regulation of Oocyte Viability by Granulosa Cell ContactJohn Peluso; Fiscal Year: 2004..abstract_text> ..
- Protein Kinase G Regulation of Granulosa Cell ViabilityJohn Peluso; Fiscal Year: 2006..We now propose to test this hypothesis using biochemical, genetic, imaging and cell biological techniques. ..
- Progesterone regulation of human luteal cell viabilityJohn Peluso; Fiscal Year: 2006..Such selective manipulation of the P4's actions could have far reaching effects on the treatment for infertility, contraception and certain types of cancers. ..
- PAIRBP & PGRMC1 act as a membrane receptor complex to mediate P4's ovarian actionJohn Peluso; Fiscal Year: 2007..These new pharmacological agents could have utility in the development contraceptives and in treating infertility and some forms of cancer. ..
- PAIRBP & PGRMC1 act as a membrane receptor complex to mediate P4's ovarian actionJohn Peluso; Fiscal Year: 2009..These new pharmacological agents could have utility in the development contraceptives and in treating infertility and some forms of cancer. ..
- PAIRBP & PGRMC1 act as a membrane receptor complex to mediate P4's ovarian actionJohn J Peluso; Fiscal Year: 2010..These new pharmacological agents could have utility in the development contraceptives and in treating infertility and some forms of cancer. ..
- PAIRBP & PGRMC1 act as a membrane receptor complex to mediate P4's ovarian actionJohn J Peluso; Fiscal Year: 2010..These new pharmacological agents could have utility in the development contraceptives and in treating infertility and some forms of cancer. ..