Sabine Pellett

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. doi request reprint Activity of botulinum neurotoxin type D (strain 1873) in human neurons
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI, USA Electronic address
    Toxicon 101:63-9. 2015
  2. pmc Persistence of botulinum neurotoxin a subtypes 1-5 in primary rat spinal cord cells
    Regina Clare Meyer Whitemarsh
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 9:e90252. 2014
  3. pmc Progress in cell based assays for botulinum neurotoxin detection
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin, Madison, WI 53706, USA
    Curr Top Microbiol Immunol 364:257-85. 2013
  4. ncbi request reprint Mak16p is required for the maturation of 25S and 5.8S rRNAs in the yeast Saccharomyces cerevisiae
    Sabine Pellett
    Department of Comparative Biosciences and Molecular and Environmental Toxicology Center, University of Wisconsin at Madison, 53706, USA
    Yeast 23:495-506. 2006
  5. doi request reprint Learning from the past: historical aspects of bacterial toxins as pharmaceuticals
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin, Madison, Madison, WI 53706, USA
    Curr Opin Microbiol 15:292-9. 2012
  6. pmc Characterization of botulinum neurotoxin A subtypes 1 through 5 by investigation of activities in mice, in neuronal cell cultures, and in vitro
    Regina C M Whitemarsh
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin, USA
    Infect Immun 81:3894-902. 2013
  7. pmc A neuronal cell-based botulinum neurotoxin assay for highly sensitive and specific detection of neutralizing serum antibodies
    Sabine Pellett
    Food Research Institute, Department of Bacteriology, University of Wisconsin, Madison, WI 53706, United States
    FEBS Lett 581:4803-8. 2007
  8. pmc Model for studying Clostridium botulinum neurotoxin using differentiated motor neuron-like NG108-15 cells
    Regina C M Whitemarsh
    Department of Bacteriology, University of Wisconsin, Madison, Madison, WI 53706, USA
    Biochem Biophys Res Commun 427:426-30. 2012
  9. pmc Botulinum neurotoxin subtype A2 enters neuronal cells faster than subtype A1
    Christina L Pier
    Department of Bacteriology, University of Wisconsin, Madison, Madison, WI 53706, USA
    FEBS Lett 585:199-206. 2011
  10. pmc Sensitive and quantitative detection of botulinum neurotoxin in neurons derived from mouse embryonic stem cells
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI 53706, USA
    Biochem Biophys Res Commun 404:388-92. 2011

Collaborators

Detail Information

Publications19

  1. doi request reprint Activity of botulinum neurotoxin type D (strain 1873) in human neurons
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI, USA Electronic address
    Toxicon 101:63-9. 2015
    ..Finally, examination of BoNT/D activity in various rodent and human cell models resulted in dramatic differences in sensitivity, indicating a unique cell entry mechanism of BoNT/D. ..
  2. pmc Persistence of botulinum neurotoxin a subtypes 1-5 in primary rat spinal cord cells
    Regina Clare Meyer Whitemarsh
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 9:e90252. 2014
    ..An intermittent dosing with BoNT/E showed intracellular activity of the shorter acting BoNT/E for 2-3 weeks, followed by reoccurrence and persistence of BoNT/A-induced SNAP-25 cleavage products. ..
  3. pmc Progress in cell based assays for botulinum neurotoxin detection
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin, Madison, WI 53706, USA
    Curr Top Microbiol Immunol 364:257-85. 2013
    ..Such quantitative and reliable BoNT potency determination is a crucial step in basic research, in the development of pharmaceutical BoNTs, and in the quantitative detection of neutralizing antibodies...
  4. ncbi request reprint Mak16p is required for the maturation of 25S and 5.8S rRNAs in the yeast Saccharomyces cerevisiae
    Sabine Pellett
    Department of Comparative Biosciences and Molecular and Environmental Toxicology Center, University of Wisconsin at Madison, 53706, USA
    Yeast 23:495-506. 2006
    ..Association of Mak16p with the 66S preribosomal complex does not appear to be sufficient for its function, because the mutant Mak16-1p protein was detected in sucrose density gradient fractions corresponding to the 66S pre-RNP complex...
  5. doi request reprint Learning from the past: historical aspects of bacterial toxins as pharmaceuticals
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin, Madison, Madison, WI 53706, USA
    Curr Opin Microbiol 15:292-9. 2012
    ....
  6. pmc Characterization of botulinum neurotoxin A subtypes 1 through 5 by investigation of activities in mice, in neuronal cell cultures, and in vitro
    Regina C M Whitemarsh
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin, USA
    Infect Immun 81:3894-902. 2013
    ..These results indicate that BoNT/A1 to -5 have distinct characteristics, which are of importance for a greater understanding of botulism and for pharmaceutical applications. ..
  7. pmc A neuronal cell-based botulinum neurotoxin assay for highly sensitive and specific detection of neutralizing serum antibodies
    Sabine Pellett
    Food Research Institute, Department of Bacteriology, University of Wisconsin, Madison, WI 53706, United States
    FEBS Lett 581:4803-8. 2007
    ..This report presents a highly sensitive and specific neuronal cell-based assay that provides sensitive and specific detection of neutralizing antibodies to BoNT/A...
  8. pmc Model for studying Clostridium botulinum neurotoxin using differentiated motor neuron-like NG108-15 cells
    Regina C M Whitemarsh
    Department of Bacteriology, University of Wisconsin, Madison, Madison, WI 53706, USA
    Biochem Biophys Res Commun 427:426-30. 2012
    ..Co-culture of the neurons with C2C12 myotubes also significantly increased BoNT/A sensitivity of NG108-15 cells (EC(50) = 26 U) in the absence of differentiation factors...
  9. pmc Botulinum neurotoxin subtype A2 enters neuronal cells faster than subtype A1
    Christina L Pier
    Department of Bacteriology, University of Wisconsin, Madison, Madison, WI 53706, USA
    FEBS Lett 585:199-206. 2011
    ..These results have important implications for the development of new BoNT based therapeutics and BoNT countermeasures...
  10. pmc Sensitive and quantitative detection of botulinum neurotoxin in neurons derived from mouse embryonic stem cells
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI 53706, USA
    Biochem Biophys Res Commun 404:388-92. 2011
    ..The data presented indicate that this assay can reliably detect BoNT/A with a similar sensitivity as the MBA...
  11. doi request reprint Human Induced Pluripotent Stem Cell Derived Neuronal Cells Cultured on Chemically-Defined Hydrogels for Sensitive In Vitro Detection of Botulinum Neurotoxin
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin at Madison, Madison, Wisconsin, United States of America
    Sci Rep 5:14566. 2015
    ..Therefore, chemically-defined synthetic hydrogels offer benefits over standard platforms when optimizing culture conditions for cell-based screening and achieve sensitivities comparable to an approved animal testing protocol. ..
  12. doi request reprint In vivo onset and duration of action varies for botulinum neurotoxin A subtypes 1-5
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI, USA Electronic address
    Toxicon 107:37-42. 2015
    ....
  13. doi request reprint Holotoxin Activity of Botulinum Neurotoxin Subtype A4 Originating from a Nontoxigenic Clostridium botulinum Expression System
    Marite Bradshaw
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin, USA
    Appl Environ Microbiol 80:7415-22. 2014
    ..In summary, a recombinant BoNT from a dual-toxin-producing strain was expressed and purified in an endogenous clostridial expression system, allowing analysis of this toxin. ..
  14. pmc Analysis of gene expression in induced pluripotent stem cell-derived human neurons exposed to botulinum neurotoxin A subtype 1 and a type A atoxic derivative
    Jacob M Scherf
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 9:e111238. 2014
    ..These data indicate a similar response of neurons to BoNT/A1 and BoNT/A ad exposure. The most highly regulated genes in cells exposed to either BoNT/A1 or BoNT/A ad are involved in neurite outgrowth and calcium channel sensitization. ..
  15. doi request reprint Comparison of the primary rat spinal cord cell (RSC) assay and the mouse bioassay for botulinum neurotoxin type A potency determination
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin, Madison, Madison, WI 53706, USA
    J Pharmacol Toxicol Methods 61:304-10. 2010
    ..A cell-based assay using primary rat spinal cord cells (RSC assay) has been previously reported to sensitively detect purified botulinum neurotoxin type A, and requires all biological properties of the toxin for detection...
  16. pmc Neuronal targeting, internalization, and biological activity of a recombinant atoxic derivative of botulinum neurotoxin A
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Dr, Madison, WI 53706, United States
    Biochem Biophys Res Commun 405:673-7. 2011
    ..Toxicity of BoNT/A ad was found to be reduced approximately 100,000-fold relative to wt BoNT/A...
  17. doi request reprint Botulinum Neurotoxins Can Enter Cultured Neurons Independent of Synaptic Vesicle Recycling
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin, 53706, United States of America
    PLoS ONE 10:e0133737. 2015
    ..Overall, our data indicate that previous intoxication of neuronal cells with BoNT does not inhibit further uptake of BoNTs. ..
  18. pmc Novel application of human neurons derived from induced pluripotent stem cells for highly sensitive botulinum neurotoxin detection
    Regina C M Whitemarsh
    Department of Bacteriology, University of Wisconsin, Madison, Madison, Wisconsin 53706, USA
    Toxicol Sci 126:426-35. 2012
    ..In summary, these data suggest that neurons derived from hiPSCs provide an ideal and highly sensitive platform for BoNT potency determination, neutralizing antibody detection and for mechanistic studies...
  19. pmc Plasmid encoded neurotoxin genes in Clostridium botulinum serotype A subtypes
    Kristin M Marshall
    Department of Bacteriology, University of Wisconsin, 1925 Willow Drive, Madison, WI 53706, USA
    Biochem Biophys Res Commun 361:49-54. 2007
    ..The finding of BoNT type A and B genes on extrachromosomal elements has important implications for the evolution of neurotoxigenicity in clostridia including the origin, expression, and lateral transfer of botulinum neurotoxin genes...