Sabine Pellett

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. pmc Progress in cell based assays for botulinum neurotoxin detection
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin, Madison, WI 53706, USA
    Curr Top Microbiol Immunol 364:257-85. 2013
  2. doi request reprint Learning from the past: historical aspects of bacterial toxins as pharmaceuticals
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin, Madison, Madison, WI 53706, USA
    Curr Opin Microbiol 15:292-9. 2012
  3. ncbi request reprint Mak16p is required for the maturation of 25S and 5.8S rRNAs in the yeast Saccharomyces cerevisiae
    Sabine Pellett
    Department of Comparative Biosciences and Molecular and Environmental Toxicology Center, University of Wisconsin at Madison, 53706, USA
    Yeast 23:495-506. 2006
  4. pmc Model for studying Clostridium botulinum neurotoxin using differentiated motor neuron-like NG108-15 cells
    Regina C M Whitemarsh
    Department of Bacteriology, University of Wisconsin, Madison, Madison, WI 53706, USA
    Biochem Biophys Res Commun 427:426-30. 2012
  5. pmc Sensitive and quantitative detection of botulinum neurotoxin in neurons derived from mouse embryonic stem cells
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI 53706, USA
    Biochem Biophys Res Commun 404:388-92. 2011
  6. pmc Botulinum neurotoxin subtype A2 enters neuronal cells faster than subtype A1
    Christina L Pier
    Department of Bacteriology, University of Wisconsin, Madison, Madison, WI 53706, USA
    FEBS Lett 585:199-206. 2011
  7. doi request reprint Comparison of the primary rat spinal cord cell (RSC) assay and the mouse bioassay for botulinum neurotoxin type A potency determination
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin, Madison, Madison, WI 53706, USA
    J Pharmacol Toxicol Methods 61:304-10. 2010
  8. pmc Neuronal targeting, internalization, and biological activity of a recombinant atoxic derivative of botulinum neurotoxin A
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Dr, Madison, WI 53706, United States
    Biochem Biophys Res Commun 405:673-7. 2011
  9. pmc Novel application of human neurons derived from induced pluripotent stem cells for highly sensitive botulinum neurotoxin detection
    Regina C M Whitemarsh
    Department of Bacteriology, University of Wisconsin, Madison, Madison, Wisconsin 53706, USA
    Toxicol Sci 126:426-35. 2012
  10. pmc A neuronal cell-based botulinum neurotoxin assay for highly sensitive and specific detection of neutralizing serum antibodies
    Sabine Pellett
    Food Research Institute, Department of Bacteriology, University of Wisconsin, Madison, WI 53706, United States
    FEBS Lett 581:4803-8. 2007

Collaborators

Detail Information

Publications10

  1. pmc Progress in cell based assays for botulinum neurotoxin detection
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin, Madison, WI 53706, USA
    Curr Top Microbiol Immunol 364:257-85. 2013
    ..Such quantitative and reliable BoNT potency determination is a crucial step in basic research, in the development of pharmaceutical BoNTs, and in the quantitative detection of neutralizing antibodies...
  2. doi request reprint Learning from the past: historical aspects of bacterial toxins as pharmaceuticals
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin, Madison, Madison, WI 53706, USA
    Curr Opin Microbiol 15:292-9. 2012
    ....
  3. ncbi request reprint Mak16p is required for the maturation of 25S and 5.8S rRNAs in the yeast Saccharomyces cerevisiae
    Sabine Pellett
    Department of Comparative Biosciences and Molecular and Environmental Toxicology Center, University of Wisconsin at Madison, 53706, USA
    Yeast 23:495-506. 2006
    ..Association of Mak16p with the 66S preribosomal complex does not appear to be sufficient for its function, because the mutant Mak16-1p protein was detected in sucrose density gradient fractions corresponding to the 66S pre-RNP complex...
  4. pmc Model for studying Clostridium botulinum neurotoxin using differentiated motor neuron-like NG108-15 cells
    Regina C M Whitemarsh
    Department of Bacteriology, University of Wisconsin, Madison, Madison, WI 53706, USA
    Biochem Biophys Res Commun 427:426-30. 2012
    ..Co-culture of the neurons with C2C12 myotubes also significantly increased BoNT/A sensitivity of NG108-15 cells (EC(50) = 26 U) in the absence of differentiation factors...
  5. pmc Sensitive and quantitative detection of botulinum neurotoxin in neurons derived from mouse embryonic stem cells
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI 53706, USA
    Biochem Biophys Res Commun 404:388-92. 2011
    ..The data presented indicate that this assay can reliably detect BoNT/A with a similar sensitivity as the MBA...
  6. pmc Botulinum neurotoxin subtype A2 enters neuronal cells faster than subtype A1
    Christina L Pier
    Department of Bacteriology, University of Wisconsin, Madison, Madison, WI 53706, USA
    FEBS Lett 585:199-206. 2011
    ..These results have important implications for the development of new BoNT based therapeutics and BoNT countermeasures...
  7. doi request reprint Comparison of the primary rat spinal cord cell (RSC) assay and the mouse bioassay for botulinum neurotoxin type A potency determination
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin, Madison, Madison, WI 53706, USA
    J Pharmacol Toxicol Methods 61:304-10. 2010
    ..A cell-based assay using primary rat spinal cord cells (RSC assay) has been previously reported to sensitively detect purified botulinum neurotoxin type A, and requires all biological properties of the toxin for detection...
  8. pmc Neuronal targeting, internalization, and biological activity of a recombinant atoxic derivative of botulinum neurotoxin A
    Sabine Pellett
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Dr, Madison, WI 53706, United States
    Biochem Biophys Res Commun 405:673-7. 2011
    ..Toxicity of BoNT/A ad was found to be reduced approximately 100,000-fold relative to wt BoNT/A...
  9. pmc Novel application of human neurons derived from induced pluripotent stem cells for highly sensitive botulinum neurotoxin detection
    Regina C M Whitemarsh
    Department of Bacteriology, University of Wisconsin, Madison, Madison, Wisconsin 53706, USA
    Toxicol Sci 126:426-35. 2012
    ..In summary, these data suggest that neurons derived from hiPSCs provide an ideal and highly sensitive platform for BoNT potency determination, neutralizing antibody detection and for mechanistic studies...
  10. pmc A neuronal cell-based botulinum neurotoxin assay for highly sensitive and specific detection of neutralizing serum antibodies
    Sabine Pellett
    Food Research Institute, Department of Bacteriology, University of Wisconsin, Madison, WI 53706, United States
    FEBS Lett 581:4803-8. 2007
    ..This report presents a highly sensitive and specific neuronal cell-based assay that provides sensitive and specific detection of neutralizing antibodies to BoNT/A...