Duanqing Pei

Summary

Affiliation: University of Minnesota
Country: USA

Publications

  1. ncbi request reprint A high-level mammalian expression system based on the Madin-Darby canine kidney cell line
    D Pei
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota, 55455, USA
    Protein Expr Purif 13:277-81. 1998
  2. ncbi request reprint Identification and characterization of the fifth membrane-type matrix metalloproteinase MT5-MMP
    D Pei
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 274:8925-32. 1999
  3. ncbi request reprint CA-MMP: a matrix metalloproteinase with a novel cysteine array, but without the classic cysteine switch
    D Pei
    Department of Pharmacology, 3 249 Millard Hall, 435 Delaware St S E, University of Minnesota, Minneapolis, MN, USA
    FEBS Lett 457:262-70. 1999
  4. ncbi request reprint Leukolysin/MMP25/MT6-MMP: a novel matrix metalloproteinase specifically expressed in the leukocyte lineage
    D Pei
    Department of Pharmacology, University of Minnesota, Minneapolis 55455, USA
    Cell Res 9:291-303. 1999
  5. ncbi request reprint Cysteine array matrix metalloproteinase (CA-MMP)/MMP-23 is a type II transmembrane matrix metalloproteinase regulated by a single cleavage for both secretion and activation
    D Pei
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 275:33988-97. 2000
  6. ncbi request reprint The hemopexin domain of membrane-type matrix metalloproteinase-1 (MT1-MMP) Is not required for its activation of proMMP2 on cell surface but is essential for MT1-MMP-mediated invasion in three-dimensional type I collagen
    Ping Wang
    Department of Pharmacology, University of Minnesota School of Medicine, Minneapolis, Minnesota 55455, USA
    J Biol Chem 279:51148-55. 2004
  7. pmc Regulation of MT1-MMP activity by β-catenin in MDCK non-cancer and HT1080 cancer cells
    Ping Liu
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota, USA
    J Cell Physiol 225:810-21. 2010
  8. ncbi request reprint Mint-3 regulates the retrieval of the internalized membrane-type matrix metalloproteinase, MT5-MMP, to the plasma membrane by binding to its carboxyl end motif EWV
    Ping Wang
    Department of Pharmacology, University of Minnesota School of Medicine, Minneapolis, MN 55455, USA
    J Biol Chem 279:20461-70. 2004
  9. doi request reprint Increased aggressiveness of human prostate PC-3 tumor cells expressing cell surface localized membrane type-1 matrix metalloproteinase (MT1-MMP)
    Xing Wang
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota, USA
    J Androl 30:259-74. 2009
  10. ncbi request reprint Complete restoration of cell surface activity of transmembrane-truncated MT1-MMP by a glycosylphosphatidylinositol anchor. Implications for MT1-MMP-mediated prommp2 activation and collagenolysis in three-dimensions
    Jing Nie
    Department of Pharmacology, University of Minnesota School of Medicine, Minneapolis, Minnesota 55455, USA
    J Biol Chem 282:6438-43. 2007

Collaborators

Detail Information

Publications37

  1. ncbi request reprint A high-level mammalian expression system based on the Madin-Darby canine kidney cell line
    D Pei
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota, 55455, USA
    Protein Expr Purif 13:277-81. 1998
    ..This system may be employed for the production of human proteins of special interests, such as those for structural determination or therapeutical development...
  2. ncbi request reprint Identification and characterization of the fifth membrane-type matrix metalloproteinase MT5-MMP
    D Pei
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 274:8925-32. 1999
    ..Taken together, these properties serve to distinguish MT5-MMP as a versatile MT-MMP playing an important role in extracellular matrix remodeling events in the brain and during embryonic development...
  3. ncbi request reprint CA-MMP: a matrix metalloproteinase with a novel cysteine array, but without the classic cysteine switch
    D Pei
    Department of Pharmacology, 3 249 Millard Hall, 435 Delaware St S E, University of Minnesota, Minneapolis, MN, USA
    FEBS Lett 457:262-70. 1999
    ..Taken together, it is concluded that CA-MMP is an MMP with distinct structure, biochemical properties and evolutionary history that may define a new subclass of the MMP superfamily...
  4. ncbi request reprint Leukolysin/MMP25/MT6-MMP: a novel matrix metalloproteinase specifically expressed in the leukocyte lineage
    D Pei
    Department of Pharmacology, University of Minnesota, Minneapolis 55455, USA
    Cell Res 9:291-303. 1999
    ..Taken together, leukolysin may be part of the proteolytic arsenal deployed by leukocytes during inflammatory responses. Molecular cloning of a novel MMP: MMP:25..
  5. ncbi request reprint Cysteine array matrix metalloproteinase (CA-MMP)/MMP-23 is a type II transmembrane matrix metalloproteinase regulated by a single cleavage for both secretion and activation
    D Pei
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 275:33988-97. 2000
    ..Thus, CA-MMP is a type II transmembrane MMP that can be regulated by a single proteolytic cleavage for both activation and secretion, establishing a novel paradigm for protein trafficking and processing within the secretory pathway...
  6. ncbi request reprint The hemopexin domain of membrane-type matrix metalloproteinase-1 (MT1-MMP) Is not required for its activation of proMMP2 on cell surface but is essential for MT1-MMP-mediated invasion in three-dimensional type I collagen
    Ping Wang
    Department of Pharmacology, University of Minnesota School of Medicine, Minneapolis, Minnesota 55455, USA
    J Biol Chem 279:51148-55. 2004
    ....
  7. pmc Regulation of MT1-MMP activity by β-catenin in MDCK non-cancer and HT1080 cancer cells
    Ping Liu
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota, USA
    J Cell Physiol 225:810-21. 2010
    ..These differences were most probably due to different subcellular locations and different involved pathways of β-catenin in these cells...
  8. ncbi request reprint Mint-3 regulates the retrieval of the internalized membrane-type matrix metalloproteinase, MT5-MMP, to the plasma membrane by binding to its carboxyl end motif EWV
    Ping Wang
    Department of Pharmacology, University of Minnesota School of Medicine, Minneapolis, MN 55455, USA
    J Biol Chem 279:20461-70. 2004
    ..Furthermore, Mint-3 significantly increased the level of MT5-MMP on the cell surface without affecting its synthesis and internalization. Therefore, Mints may be the adaptor proteins that regulate the trafficking of MT-MMPs...
  9. doi request reprint Increased aggressiveness of human prostate PC-3 tumor cells expressing cell surface localized membrane type-1 matrix metalloproteinase (MT1-MMP)
    Xing Wang
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota, USA
    J Androl 30:259-74. 2009
    ....
  10. ncbi request reprint Complete restoration of cell surface activity of transmembrane-truncated MT1-MMP by a glycosylphosphatidylinositol anchor. Implications for MT1-MMP-mediated prommp2 activation and collagenolysis in three-dimensions
    Jing Nie
    Department of Pharmacology, University of Minnesota School of Medicine, Minneapolis, Minnesota 55455, USA
    J Biol Chem 282:6438-43. 2007
    ..Our results suggest that both membrane-tethering and proteolytic activity encoded by MT1-MMP are required for its ability to promote cell growth and invasion in a three-dimensional collagen matrix...
  11. ncbi request reprint Limited processing of pro-matrix metalloprotease-2 (gelatinase A) overexpressed by transfection in PC-3 human prostate tumor cells: association with restricted cell surface localization of membrane-type matrix metalloproteinase-1
    Michael J Wilson
    Minneapolis VA Medical Center, and Department of Laboratory Medicine and Pathology, and Minnesoata Cancer Center, University of Minnesota, Minneapolis, USA
    J Androl 25:274-85. 2004
    ..The molecular basis for the low level of processing of pro-MMP-2 by PC-3 cells may be due to an overabundance of TIMP-2 and/or a low level of cell surface active MT1-MMP...
  12. ncbi request reprint Membrane type-1 matrix metalloproteinase promotes human melanoma invasion and growth
    Joji Iida
    Department of Laboratory Medicine and Pathology, and University of Minnesota Cancer Center, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Invest Dermatol 122:167-76. 2004
    ..The results suggest a more general role for elevated MT1-MMP in promoting both the selective invasion and increased growth of malignant melanoma in vivo...
  13. ncbi request reprint Direct activation of pro-matrix metalloproteinase-2 by leukolysin/membrane-type 6 matrix metalloproteinase/matrix metalloproteinase 25 at the asn(109)-Tyr bond
    Jing Nie
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Cancer Res 63:6758-62. 2003
    ..Taken these facts together, we conclude that MT6-MMP may participate in tumor invasion and metastasis by directly converting proMMP-2 into active form...
  14. ncbi request reprint Proprotein convertase furin interacts with and cleaves pro-ADAMTS4 (Aggrecanase-1) in the trans-Golgi network
    Ping Wang
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 279:15434-40. 2004
    ..These results indicate that the activation mechanism for ADAMTS4 can be targeted for therapeutical intervention against this enzyme...
  15. ncbi request reprint Activation of membrane-type matrix metalloproteinase 3 zymogen by the proprotein convertase furin in the trans-Golgi network
    Tiebang Kang
    Department of Pharmacology, University of Minnesota, 321 Church Street S E, 6 120 Jackson Hall, Minneapolis, MN 55455, USA
    Cancer Res 62:675-81. 2002
    ..Thus, furin processes MT3-MMP zymogen in the trans-Golgi network, where they colocalize independently of their apparent enzyme-substrate relationship...
  16. ncbi request reprint Rapid inactivation of alpha-1-proteinase inhibitor by neutrophil specific leukolysin/membrane-type matrix metalloproteinase 6
    Jing Nie
    Department of Pharmacology, University of Minnesota, Minneapolis, MN 55455, USA
    Exp Cell Res 296:145-50. 2004
    ..Taken together, these results suggest that neutrophils may mediate tissue destruction by deploying leukolysin to weaken the alpha1-PI protective shield at inflammatory sites...
  17. pmc Fluidic and air-stable supported lipid bilayer and cell-mimicking microarrays
    Yang Deng
    Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Am Chem Soc 130:6267-71. 2008
    ..These demonstrations establish the viability of the fluidic and air-stable SLB platform for generating content microarrays in high throughput studies, e.g., the screening of drugs and nanomedicine targeting cell surface receptors...
  18. ncbi request reprint Distinct roles of catalytic and pexin-like domains in membrane-type matrix metalloproteinase (MMP)-mediated pro-MMP-2 activation and collagenolysis
    Aixiang Jiang
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 278:38765-71. 2003
    ..Natl. Acad. Sci. U. S. A. 98, 13693-13698). Taken together, these results demonstrate that domains in MT-MMPs function differently toward a given substrate and thus should be targeted differentially for future therapeutic development...
  19. ncbi request reprint Immobilization of oriented protein molecules on poly(ethylene glycol)-coated Si(111)
    Taewoon Cha
    Department of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA
    Proteomics 4:1965-76. 2004
    ..This kind of quantitative analysis is essential in tuning surface concentration and chemical environment for optimal sensitivity in probe-target interaction...
  20. ncbi request reprint Co-recycling of MT1-MMP and MT3-MMP through the trans-Golgi network. Identification of DKV582 as a recycling signal
    Xing Wang
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 279:9331-6. 2004
    ..These results suggest that MT-MMPs may coordinate their proteolytic activities through the cellular trafficking machinery...
  21. ncbi request reprint Matrix metalloproteinases target protease-activated receptors on the tumor cell surface
    Duanqing Pei
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Cancer Cell 7:207-8. 2005
    ..This MMP-PAR axis may represent a novel signaling pathway communicating between tumor and stromal cells during tumor progression...
  22. ncbi request reprint Differential inhibition of membrane type 3 (MT3)-matrix metalloproteinase (MMP) and MT1-MMP by tissue inhibitor of metalloproteinase (TIMP)-2 and TIMP-3 rgulates pro-MMP-2 activation
    Huiren Zhao
    Department of Pathology, School of Medicine, Wayne State University, Detroit, Michigan 48201, USA
    J Biol Chem 279:8592-601. 2004
    ..These results demonstrate that TIMP-3 is a major regulator of MT3-MMP activity and further underscores the unique interactions of TIMPs with MT-MMPs in the control of pericellular proteolysis...
  23. ncbi request reprint Expression of matrix metalloproteinase-2 and -9 and their inhibitors, tissue inhibitor of metalloproteinase-1 and -2, in primary cultures of human prostatic stromal and epithelial cells
    Michael J Wilson
    Minneapolis VA Medical Center, Minnesota 55417, USA
    J Cell Physiol 191:208-16. 2002
    ..Our results suggest that the elevated levels of MMP-2 and -9 observed in prostate development and cancer may be due to the elevated TGF-beta associated with these tissues...
  24. ncbi request reprint Oligomerization through hemopexin and cytoplasmic domains regulates the activity and turnover of membrane-type 1 matrix metalloproteinase
    Kaisa Lehti
    Department of Virology, Haartman Institute, Biomedicum Helsinki, University of Helsinki and Helsinki University Hospital, FIN 00014 Helsinki, Finland
    J Biol Chem 277:8440-8. 2002
    ..Copurification of MT1-MMP with these fusion proteins correlated with their cell-surface co-localization. Thus, MT1-MMP oligomerization through the hemopexin, transmembrane, and cytoplasmic domains controls its catalytic activity...
  25. ncbi request reprint Membrane type-matrix metalloproteinases (MT-MMP)
    Stanley Zucker
    VA Medical Center, Northport, New York 11768, USA
    Curr Top Dev Biol 54:1-74. 2003
  26. pmc Differentiation of secreted and membrane-type matrix metalloproteinase activities based on substitutions and interruptions of triple-helical sequences
    Dmitriy Minond
    Department of Chemistry and Biochemistry, Florida Atlantic University, 777 Glades Road, Boca Raton, Florida 33431 0991, USA
    Biochemistry 46:3724-33. 2007
    ..Such differences may be significant for understanding MMP mechanisms of action and aid in the development of selective MMP inhibitors...
  27. ncbi request reprint A dominant-negative form of mouse SOX2 induces trophectoderm differentiation and progressive polyploidy in mouse embryonic stem cells
    Jun Li
    Institute of Pharmacology, Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institutes of Biomedicine, School of Medicine, Tsinghua University, Beijing 100084, China
    J Biol Chem 282:19481-92. 2007
    ..These results suggest that SOX2 maintains stem cell pluripotency by shuttling between the nucleus and cytoplasm in cooperation with OCT4 to prevent trophectoderm differentiation and polyploid formation in ES cells...
  28. ncbi request reprint Stem cell pluripotency and transcription factor Oct4
    Guang Jin Pan
    Department of Pharmacology, School of Sciences, Tsinghua University, Beijing 100084, China
    Cell Res 12:321-9. 2002
    ..In this review, we summarize the structure and function of Oct4 and address issues related to Oct4 function in maintaining totipotency or pluripotency of embryonic stem cells...
  29. ncbi request reprint Nuclear localization of the phosphatidylserine receptor protein via multiple nuclear localization signals
    Ping Cui
    Institute of Pharmacology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua Institutes of Biomedical Sciences, Tsinghua University, Beijing, China
    Exp Cell Res 293:154-63. 2004
    ..Finally, multiple nuclear localization signals were identified in PSR sequence, each capable of targeting GFP to the nuclei. Together, these results suggest that PSR may serve a dual role both on the cell surface and in the nuclei...
  30. ncbi request reprint The roles of substrate thermal stability and P2 and P1' subsite identity on matrix metalloproteinase triple-helical peptidase activity and collagen specificity
    Dmitriy Minond
    Department of Chemistry and Biochemistry, Florida Atlantic University, 777 Glades Road, Boca Raton, FL 33431 0991, USA
    J Biol Chem 281:38302-13. 2006
    ..Further exploration of MMP active sites and exosites, in combination with substrate conformation, may prove valuable for additional dissection of collagenolysis and yield information useful in the design of more selective MMP inhibitors...
  31. ncbi request reprint Sorting nexin 10 induces giant vacuoles in mammalian cells
    Baoming Qin
    Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China
    J Biol Chem 281:36891-6. 2006
    ..Brefeldin A, a chemical known to block the endoplasmic reticulum to Golgi transport, inhibited the vacuolization process. Together, these results suggest that SNX10 activity may be involved in the regulation of endosome homeostasis...
  32. ncbi request reprint Regulation of the pluripotency marker Rex-1 by Nanog and Sox2
    Wenjing Shi
    Institute of Pharmacology, Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institutes of Biomedicine, School of Medicine, Tsinghua University, Beijing 100084, China
    J Biol Chem 281:23319-25. 2006
    ..Taking these findings together, we conclude that Rex-1 is a direct target of Nanog, which is augmented by Sox2 and Oct-3/4...
  33. pmc Design of wide-spectrum inhibitors targeting coronavirus main proteases
    HaiTao Yang
    Tsinghua IBP Joint Research Group for Structural Biology, Tsinghua University, Beijing, China
    PLoS Biol 3:e324. 2005
    ..Further modification could rapidly lead to the discovery of a single agent with clinical potential against existing and possible future emerging CoV-related diseases...
  34. ncbi request reprint Genomic instability in laminopathy-based premature aging
    Baohua Liu
    Department of Biochemistry, University of Hong Kong, 21 Sassoon Road, Hong Kong
    Nat Med 11:780-5. 2005
    ..Our results indicate that unprocessed prelamin A and truncated lamin A act dominant negatively to perturb DNA damage response and repair, resulting in genomic instability which might contribute to laminopathy-based premature aging...
  35. ncbi request reprint Total synthesis of cyclic tetrapeptide FR235222, a potent immunosuppressant that inhibits mammalian histone deacetylases
    Weiqing Xie
    State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 354 Fenglin Lu, Shanghai 200032, China
    Org Lett 7:2775-7. 2005
    ....
  36. ncbi request reprint Identification of a nuclear localization signal in OCT4 and generation of a dominant negative mutant by its ablation
    Guangjin Pan
    Institute of Pharmacology and State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China
    J Biol Chem 279:37013-20. 2004
    ..These data suggest that this dominant negative form of OCT4 may be a useful tool for modulating the activity of OCT4 in pluripotent cells such as embryonic stem cells to achieve the desired cell types for therapeutic applications...
  37. ncbi request reprint Intracellular activation of human adamalysin 19/disintegrin and metalloproteinase 19 by furin occurs via one of the two consecutive recognition sites
    Tiebang Kang
    Department of Chemistry and Biochemistry and Institute of Molecular Biophysics, Florida State University, Tallahassee 32306 4390, USA
    J Biol Chem 277:25583-91. 2002
    ..This report is the first thorough investigation of the intracellular activation of adamalysin 19, demonstrating that furin activated pro-hADAM19 in the secretory pathway via one of the two consecutive furin recognition sites...