Youri Pavlov

Summary

Affiliation: University of Nebraska Medical Center
Country: USA

Publications

  1. Rogozin I, Roche Lima A, Lada A, Belinky F, Sidorenko I, Glazko G, et al. Nucleotide Weight Matrices Reveal Ubiquitous Mutational Footprints of AID/APOBEC Deaminases in Human Cancer Genomes. Cancers (Basel). 2019;11: pubmed publisher
    ..Overall, the weight matrix approach reveals that somatic mutations are significantly associated with at least one AID/APOBEC mutable motif in all studied cancers. ..
  2. Stepchenkova E, Shiriaeva A, Pavlov Y. Deletion of the DEF1 gene does not confer UV-immutability but frequently leads to self-diploidization in yeast Saccharomyces cerevisiae. DNA Repair (Amst). 2018;70:49-54 pubmed publisher
    ..We conclude that Def1 does not play a critical role in damage-induced mutagenesis. ..
  3. Baranovskiy A, Siebler H, Pavlov Y, Tahirov T. Iron-Sulfur Clusters in DNA Polymerases and Primases of Eukaryotes. Methods Enzymol. 2018;599:1-20 pubmed publisher
    ..In DNA polymerases δ and ζ, they can potentially play regulatory role by sensing hurdles during DNA replication and assisting with DNA polymerase switches by oscillation between oxidized-reduced states. ..
  4. Babudri N, Achilli A, Martinelli C, Moore E, Lancioni H, Pavlov Y. The role of DNA polymerase alpha in the control of mutagenesis in Saccharomyces cerevisiae cells starved for nutrients. Ekol Genet. 2011;9:53-61 pubmed
    ..Here we provide evidence that Pol ? is involved in the control of SAM in yeast cells starved for adenine by participation in sporadic replication and/or DNA repair under these conditions. ..
  5. Mayorov V, Rogozin I, Adkison L, Frahm C, Kunkel T, Pavlov Y. Expression of human AID in yeast induces mutations in context similar to the context of somatic hypermutation at G-C pairs in immunoglobulin genes. BMC Immunol. 2005;6:10 pubmed
    ..This indicates that the intrinsic substrate specificity of AID itself is a primary determinant of mutational hotspots at G-C base pairs during SHM. ..
  6. Stepchenkova E, Kozmin S, Alenin V, Pavlov Y. Genome-wide screening for genes whose deletions confer sensitivity to mutagenic purine base analogs in yeast. BMC Genet. 2005;6:31 pubmed
    ..Three of them also protect from AHA. ..
  7. Pavlov Y, Maki S, Maki H, Kunkel T. Evidence for interplay among yeast replicative DNA polymerases alpha, delta and epsilon from studies of exonuclease and polymerase active site mutations. BMC Biol. 2004;2:11 pubmed
    ..This suggests that other polymerases can substitute for certain functions of polymerase epsilon. ..
  8. Siebler H, Lada A, Baranovskiy A, Tahirov T, Pavlov Y. A novel variant of DNA polymerase ?, Rev3?C, highlights differential regulation of Pol32 as a subunit of polymerase ? versus ? in Saccharomyces cerevisiae. DNA Repair (Amst). 2014;24:138-149 pubmed publisher
    ..The evidence for differential regulation of Pol32 in pol ? and pol ? emphasizes the complexity of polymerase switches. ..
  9. Zhang Y, Baranovskiy A, Tahirov T, Pavlov Y. The C-terminal domain of the DNA polymerase catalytic subunit regulates the primase and polymerase activities of the human DNA polymerase ?-primase complex. J Biol Chem. 2014;289:22021-34 pubmed publisher
    ..We have found that pol? activity strongly depends on the sequence of the template and that homopyrimidine runs create a strong barrier for DNA synthesis by pol?. ..

More Information

Publications11

  1. Zhang Y, Baranovskiy A, Tahirov E, Tahirov T, Pavlov Y. Divalent ions attenuate DNA synthesis by human DNA polymerase ? by changing the structure of the template/primer or by perturbing the polymerase reaction. DNA Repair (Amst). 2016;43:24-33 pubmed publisher
    ..It is possible that Zn(2+) perturbs metal-mediated transactions in pol active site, for example affecting the step of pyrophosphate removal at the end of each pol cycle necessary for continuation of polymerization. ..
  2. Stepchenkova E, Tarakhovskaya E, Siebler H, Pavlov Y. Defect of Fe-S cluster binding by DNA polymerase ? in yeast suppresses UV-induced mutagenesis, but enhances DNA polymerase ? - dependent spontaneous mutagenesis. DNA Repair (Amst). 2017;49:60-69 pubmed publisher
    ..These results reveal differential control of the two major types of pol ?-dependent mutagenesis by the Fe-S cluster present in replicative pol ?. ..